Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87768.t1 | XP_003623535.1 | 86.5 | 170 | 23 | 0 | 1 | 170 | 43 | 212 | 4.50E-74 | 287.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87768.t1 | Q9LZX1 | 58.0 | 169 | 71 | 0 | 1 | 169 | 47 | 215 | 2.0e-54 | 213.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87768.t1 | G7KW17 | 86.5 | 170 | 23 | 0 | 1 | 170 | 43 | 212 | 3.2e-74 | 287.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene42097 | MS.gene87768 | PPI |
| MS.gene009719 | MS.gene87768 | PPI |
| MS.gene87769 | MS.gene87768 | PPI |
| MS.gene87768 | MS.gene87762 | PPI |
| MS.gene99704 | MS.gene87768 | PPI |
| MS.gene26305 | MS.gene87768 | PPI |
| MS.gene87039 | MS.gene87768 | PPI |
| MS.gene42848 | MS.gene87768 | PPI |
| MS.gene87768 | MS.gene87038 | PPI |
| MS.gene42103 | MS.gene87768 | PPI |
| MS.gene87038 | MS.gene87768 | PPI |
| MS.gene42098 | MS.gene87768 | PPI |
| MS.gene87043 | MS.gene87768 | PPI |
| MS.gene87040 | MS.gene87768 | PPI |
| MS.gene87765 | MS.gene87768 | PPI |
| MS.gene051028 | MS.gene87768 | PPI |
| MS.gene87767 | MS.gene87768 | PPI |
| MS.gene87768 | MS.gene42096 | PPI |
| MS.gene42099 | MS.gene87768 | PPI |
| MS.gene42091 | MS.gene87768 | PPI |
| MS.gene91237 | MS.gene87768 | PPI |
| MS.gene42100 | MS.gene87768 | PPI |
| MS.gene87041 | MS.gene87768 | PPI |
| MS.gene99705 | MS.gene87768 | PPI |
| MS.gene009718 | MS.gene87768 | PPI |
| MS.gene87768 | MS.gene91238 | PPI |
| MS.gene87768 | MS.gene25045 | PPI |
| MS.gene42096 | MS.gene87768 | PPI |
| MS.gene87768 | MS.gene87046 | PPI |
| MS.gene87768 | MS.gene81036 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87768.t1 | MTR_7g072120 | 97.647 | 170 | 4 | 0 | 1 | 170 | 43 | 212 | 1.85e-122 | 343 |
| MS.gene87768.t1 | MTR_7g072170 | 87.574 | 169 | 21 | 0 | 1 | 169 | 43 | 211 | 2.76e-108 | 308 |
| MS.gene87768.t1 | MTR_7g072140 | 85.294 | 170 | 25 | 0 | 1 | 170 | 43 | 212 | 4.85e-107 | 305 |
| MS.gene87768.t1 | MTR_7g072270 | 82.353 | 170 | 29 | 1 | 1 | 170 | 42 | 210 | 8.78e-103 | 294 |
| MS.gene87768.t1 | MTR_7g072250 | 75.148 | 169 | 37 | 2 | 1 | 169 | 77 | 240 | 3.47e-88 | 258 |
| MS.gene87768.t1 | MTR_7g072270 | 82.400 | 125 | 21 | 1 | 1 | 125 | 42 | 165 | 4.65e-74 | 219 |
| MS.gene87768.t1 | MTR_4g029430 | 63.095 | 168 | 56 | 3 | 2 | 168 | 36 | 198 | 5.77e-73 | 218 |
| MS.gene87768.t1 | MTR_7g071710 | 83.761 | 117 | 19 | 0 | 52 | 168 | 1 | 117 | 7.92e-73 | 214 |
| MS.gene87768.t1 | MTR_1g014180 | 55.346 | 159 | 64 | 3 | 7 | 162 | 31 | 185 | 1.15e-52 | 166 |
| MS.gene87768.t1 | MTR_1g014150 | 47.771 | 157 | 74 | 4 | 8 | 162 | 39 | 189 | 6.60e-40 | 134 |
| MS.gene87768.t1 | MTR_1g014160 | 41.954 | 174 | 76 | 6 | 7 | 162 | 74 | 240 | 1.31e-27 | 103 |
| MS.gene87768.t1 | MTR_2g103840 | 33.136 | 169 | 105 | 5 | 3 | 167 | 40 | 204 | 6.09e-25 | 96.3 |
| MS.gene87768.t1 | MTR_5g074550 | 35.119 | 168 | 95 | 5 | 3 | 161 | 29 | 191 | 1.68e-24 | 94.7 |
| MS.gene87768.t1 | MTR_4g010180 | 29.518 | 166 | 111 | 5 | 3 | 165 | 29 | 191 | 1.45e-22 | 89.7 |
| MS.gene87768.t1 | MTR_2g103850 | 30.120 | 166 | 108 | 5 | 7 | 168 | 45 | 206 | 2.34e-20 | 84.3 |
| MS.gene87768.t1 | MTR_5g074560 | 31.056 | 161 | 101 | 5 | 3 | 161 | 32 | 184 | 8.19e-20 | 82.0 |
| MS.gene87768.t1 | MTR_0650s0010 | 29.070 | 172 | 107 | 6 | 3 | 162 | 29 | 197 | 3.26e-16 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87768.t1 | AT5G01750 | 64.497 | 169 | 60 | 0 | 1 | 169 | 47 | 215 | 1.26e-86 | 253 |
| MS.gene87768.t1 | AT3G11740 | 65.698 | 172 | 57 | 2 | 1 | 170 | 23 | 194 | 6.27e-81 | 238 |
| MS.gene87768.t1 | AT2G14560 | 54.819 | 166 | 75 | 0 | 1 | 166 | 27 | 192 | 2.19e-66 | 201 |
| MS.gene87768.t1 | AT2G14560 | 55.828 | 163 | 72 | 0 | 1 | 163 | 87 | 249 | 3.44e-65 | 201 |
| MS.gene87768.t1 | AT1G33840 | 53.988 | 163 | 75 | 0 | 1 | 163 | 47 | 209 | 8.30e-65 | 198 |
| MS.gene87768.t1 | AT5G01750 | 63.492 | 126 | 46 | 0 | 1 | 126 | 47 | 172 | 6.80e-62 | 189 |
| MS.gene87768.t1 | AT2G14560 | 51.095 | 137 | 67 | 0 | 1 | 137 | 27 | 163 | 3.56e-51 | 162 |
| MS.gene87768.t1 | AT2G14560 | 51.095 | 137 | 67 | 0 | 1 | 137 | 87 | 223 | 4.67e-50 | 161 |
| MS.gene87768.t1 | AT1G33840 | 50.388 | 129 | 64 | 0 | 1 | 129 | 47 | 175 | 5.72e-48 | 154 |
| MS.gene87768.t1 | AT3G16900 | 45.860 | 157 | 73 | 3 | 10 | 165 | 35 | 180 | 4.59e-47 | 152 |
| MS.gene87768.t1 | AT3G56180 | 35.583 | 163 | 99 | 3 | 8 | 167 | 45 | 204 | 2.68e-30 | 109 |
| MS.gene87768.t1 | AT3G56180 | 34.969 | 163 | 98 | 4 | 8 | 167 | 45 | 202 | 4.47e-28 | 103 |
| MS.gene87768.t1 | AT3G10986 | 34.756 | 164 | 101 | 3 | 8 | 165 | 31 | 194 | 5.57e-27 | 100 |
| MS.gene87768.t1 | AT3G15810 | 31.707 | 164 | 106 | 3 | 7 | 168 | 46 | 205 | 3.62e-26 | 99.4 |
| MS.gene87768.t1 | AT1G80120 | 34.337 | 166 | 102 | 4 | 7 | 168 | 32 | 194 | 7.05e-21 | 85.5 |
| MS.gene87768.t1 | AT2G38640 | 31.481 | 162 | 101 | 6 | 5 | 163 | 37 | 191 | 1.01e-15 | 71.6 |
| MS.gene87768.t1 | AT5G20640 | 26.857 | 175 | 111 | 5 | 3 | 162 | 34 | 206 | 8.43e-14 | 67.0 |
| MS.gene87768.t1 | AT2G05910 | 27.811 | 169 | 101 | 7 | 3 | 162 | 26 | 182 | 1.54e-13 | 65.9 |
| MS.gene87768.t1 | AT2G30270 | 27.950 | 161 | 102 | 6 | 3 | 161 | 31 | 179 | 1.69e-13 | 65.5 |
Find 37 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGTCTTCATCTGGATTA+AGG | 0.216511 | 7.1:+29931240 | None:intergenic |
| AAAATCACAAACATCTTCTT+TGG | 0.257604 | 7.1:+29931192 | None:intergenic |
| TGATCGGTGGGAAGCTTATA+GGG | 0.299737 | 7.1:-29931311 | MS.gene87768:CDS |
| ATGATCGGTGGGAAGCTTAT+AGG | 0.312103 | 7.1:-29931312 | MS.gene87768:CDS |
| CATCTAATTTGGTCTTCATC+TGG | 0.334921 | 7.1:+29931233 | None:intergenic |
| AGCTAGAAACACATCTAATT+TGG | 0.366171 | 7.1:+29931222 | None:intergenic |
| CTTGCGACGGAGGGTGGTGA+TGG | 0.396422 | 7.1:+29931868 | None:intergenic |
| TTGCGACGGAGGGTGGTGAT+GGG | 0.452287 | 7.1:+29931869 | None:intergenic |
| CGTGTCTTGCTTGATGCTGC+TGG | 0.455360 | 7.1:-29931896 | MS.gene87768:CDS |
| GGCGAAGTTTCCATCTGAAA+TGG | 0.475827 | 7.1:+29931985 | None:intergenic |
| CGATCTTGCATTGTTTATGC+TGG | 0.480226 | 7.1:-29931145 | MS.gene87768:CDS |
| AACATTGTCTTCAAAGTGAA+AGG | 0.488864 | 7.1:-29931944 | MS.gene87768:CDS |
| ATGAAAGCGTAGTCAACATT+GGG | 0.498681 | 7.1:+29930911 | None:intergenic |
| AAATTAGATGTGTTTCTAGC+TGG | 0.501483 | 7.1:-29931220 | MS.gene87768:CDS |
| GATAAACCGTGACTGAGAAA+TGG | 0.513280 | 7.1:+29930933 | None:intergenic |
| CAAGCAAGACACGACGATCA+TGG | 0.522499 | 7.1:+29931906 | None:intergenic |
| TGTTGACTACGCTTTCATAG+TGG | 0.527609 | 7.1:-29930906 | MS.gene87768:CDS |
| ATCTAACCACATTGTTGCAC+AGG | 0.530405 | 7.1:-29931119 | MS.gene87768:intron |
| TATGAAAGCGTAGTCAACAT+TGG | 0.542595 | 7.1:+29930910 | None:intergenic |
| TAAAGACCATTTCTCAGTCA+CGG | 0.553557 | 7.1:-29930939 | MS.gene87768:CDS |
| TGCGACGGAGGGTGGTGATG+GGG | 0.564704 | 7.1:+29931870 | None:intergenic |
| CATCACCACCCTCCGTCGCA+AGG | 0.565107 | 7.1:-29931867 | MS.gene87768:intron |
| TAAAGTCAGAGGCAGTTGGT+TGG | 0.571221 | 7.1:-29931170 | MS.gene87768:CDS |
| AAGGTGATGACCATTTCAGA+TGG | 0.578907 | 7.1:-29931995 | None:intergenic |
| AGTGACTATGCATGATCGGT+GGG | 0.580863 | 7.1:-29931323 | MS.gene87768:CDS |
| TTGAACGTACCTTGCGACGG+AGG | 0.612314 | 7.1:+29931858 | None:intergenic |
| TAGTGACTATGCATGATCGG+TGG | 0.614019 | 7.1:-29931324 | MS.gene87768:CDS |
| TGAACGTACCTTGCGACGGA+GGG | 0.617417 | 7.1:+29931859 | None:intergenic |
| ACGTACCTTGCGACGGAGGG+TGG | 0.620243 | 7.1:+29931862 | None:intergenic |
| GATCGGTGGGAAGCTTATAG+GGG | 0.629822 | 7.1:-29931310 | MS.gene87768:CDS |
| TAATTACCTGTGCAACAATG+TGG | 0.644297 | 7.1:+29931113 | None:intergenic |
| AGATAGTGACTATGCATGAT+CGG | 0.644746 | 7.1:-29931327 | MS.gene87768:CDS |
| CAAGACACGACGATCATGGA+GGG | 0.648557 | 7.1:+29931910 | None:intergenic |
| GTTTCCGTTGACATCAGTGA+CGG | 0.656884 | 7.1:+29931964 | None:intergenic |
| TTCGCCGTCACTGATGTCAA+CGG | 0.668229 | 7.1:-29931968 | MS.gene87768:CDS |
| AAGTTGAACGTACCTTGCGA+CGG | 0.682447 | 7.1:+29931855 | None:intergenic |
| GCAAGACACGACGATCATGG+AGG | 0.713270 | 7.1:+29931909 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACTGAAATTATTAGAT+CGG | - | chr7.1:29931103-29931122 | MS.gene87768:intron | 15.0% |
| !!! | TTTTAATTAATTACGTTGAT+TGG | - | chr7.1:29931791-29931810 | MS.gene87768:intron | 15.0% |
| ! | AAAATCACAAACATCTTCTT+TGG | + | chr7.1:29931659-29931678 | None:intergenic | 25.0% |
| ! | CTATCTATCACACAAAATAA+TGG | - | chr7.1:29931161-29931180 | MS.gene87768:CDS | 25.0% |
| !! | GATAAAGCATTACTGTTAAA+TGG | + | chr7.1:29931046-29931065 | None:intergenic | 25.0% |
| !! | TAAGCACAAATCAATTTTCA+TGG | - | chr7.1:29931832-29931851 | MS.gene87768:intron | 25.0% |
| !!! | GTCAACATTTTGTTTTATGA+GGG | - | chr7.1:29931219-29931238 | MS.gene87768:CDS | 25.0% |
| AAATAACGAGATTCAAACTC+AGG | + | chr7.1:29931138-29931157 | None:intergenic | 30.0% | |
| AACATTGTCTTCAAAGTGAA+AGG | - | chr7.1:29930904-29930923 | MS.gene87768:CDS | 30.0% | |
| AGCTAGAAACACATCTAATT+TGG | + | chr7.1:29931629-29931648 | None:intergenic | 30.0% | |
| CTATCACACAAAATAATGGT+CGG | - | chr7.1:29931165-29931184 | MS.gene87768:CDS | 30.0% | |
| GATATTAGCTCGATAATACA+TGG | - | chr7.1:29931267-29931286 | MS.gene87768:CDS | 30.0% | |
| TACTGAAATTATTAGATCGG+AGG | - | chr7.1:29931106-29931125 | MS.gene87768:intron | 30.0% | |
| TATACTCTTGTATACACAAG+AGG | + | chr7.1:29931766-29931785 | None:intergenic | 30.0% | |
| ! | TACTGAATTTTGTCATGTTG+TGG | - | chr7.1:29931495-29931514 | MS.gene87768:intron | 30.0% |
| !! | AAATTAGATGTGTTTCTAGC+TGG | - | chr7.1:29931628-29931647 | MS.gene87768:intron | 30.0% |
| !! | CACTGTTACGAGTATTTTAA+TGG | - | chr7.1:29931885-29931904 | MS.gene87768:CDS | 30.0% |
| !!! | GGTCAACATTTTGTTTTATG+AGG | - | chr7.1:29931218-29931237 | MS.gene87768:CDS | 30.0% |
| !!! | GTTTGTGATTTTAAAGTCAG+AGG | - | chr7.1:29931667-29931686 | MS.gene87768:intron | 30.0% |
| AGATAGTGACTATGCATGAT+CGG | - | chr7.1:29931521-29931540 | MS.gene87768:intron | 35.0% | |
| ATTAGCTCGATAATACATGG+CGG | - | chr7.1:29931270-29931289 | MS.gene87768:CDS | 35.0% | |
| TAAAGACCATTTCTCAGTCA+CGG | - | chr7.1:29931909-29931928 | MS.gene87768:CDS | 35.0% | |
| TAATTACCTGTGCAACAATG+TGG | + | chr7.1:29931738-29931757 | None:intergenic | 35.0% | |
| ! | ATGAAAGCGTAGTCAACATT+GGG | + | chr7.1:29931940-29931959 | None:intergenic | 35.0% |
| ! | CATCTAATTTGGTCTTCATC+TGG | + | chr7.1:29931618-29931637 | None:intergenic | 35.0% |
| ! | TATGAAAGCGTAGTCAACAT+TGG | + | chr7.1:29931941-29931960 | None:intergenic | 35.0% |
| ! | TTTGGTCTTCATCTGGATTA+AGG | + | chr7.1:29931611-29931630 | None:intergenic | 35.0% |
| !!! | ATTTTAAAGTCAGAGGCAGT+TGG | - | chr7.1:29931674-29931693 | MS.gene87768:intron | 35.0% |
| ATCTAACCACATTGTTGCAC+AGG | - | chr7.1:29931729-29931748 | MS.gene87768:intron | 40.0% | |
| CGATCTTGCATTGTTTATGC+TGG | - | chr7.1:29931703-29931722 | MS.gene87768:intron | 40.0% | |
| CTAGTTAAGAATGTGACCTC+TGG | - | chr7.1:29931315-29931334 | MS.gene87768:CDS | 40.0% | |
| GATAAACCGTGACTGAGAAA+TGG | + | chr7.1:29931918-29931937 | None:intergenic | 40.0% | |
| GTAACTCATACATGCTAGAG+AGG | + | chr7.1:29931403-29931422 | None:intergenic | 40.0% | |
| GTATGAGTTACTTCATTCGC+AGG | - | chr7.1:29931412-29931431 | MS.gene87768:intron | 40.0% | |
| TCACATTCTTAACATGCCAG+AGG | + | chr7.1:29931380-29931399 | None:intergenic | 40.0% | |
| ! | TGTTGACTACGCTTTCATAG+TGG | - | chr7.1:29931942-29931961 | MS.gene87768:CDS | 40.0% |
| AAGTTGAACGTACCTTGCGA+CGG | + | chr7.1:29930996-29931015 | None:intergenic | 45.0% | |
| AGAATGTGACCTCTCTAGCA+TGG | - | chr7.1:29931322-29931341 | MS.gene87768:CDS | 45.0% | |
| AGTGACTATGCATGATCGGT+GGG | - | chr7.1:29931525-29931544 | MS.gene87768:intron | 45.0% | |
| ATGATCGGTGGGAAGCTTAT+AGG | - | chr7.1:29931536-29931555 | MS.gene87768:intron | 45.0% | |
| CTTAACATGCCATGCTAGAG+AGG | + | chr7.1:29931334-29931353 | None:intergenic | 45.0% | |
| GGCGAAGTTTCCATCTGAAA+TGG | + | chr7.1:29930866-29930885 | None:intergenic | 45.0% | |
| GTTTCCGTTGACATCAGTGA+CGG | + | chr7.1:29930887-29930906 | None:intergenic | 45.0% | |
| TAAAGTCAGAGGCAGTTGGT+TGG | - | chr7.1:29931678-29931697 | MS.gene87768:intron | 45.0% | |
| TAGTGACTATGCATGATCGG+TGG | - | chr7.1:29931524-29931543 | MS.gene87768:intron | 45.0% | |
| TGATCGGTGGGAAGCTTATA+GGG | - | chr7.1:29931537-29931556 | MS.gene87768:intron | 45.0% | |
| !! | AAATAAAATAAAACAATTAA+TGG | - | chr7.1:29931197-29931216 | MS.gene87768:CDS | 5.0% |
| CAAGACACGACGATCATGGA+GGG | + | chr7.1:29930941-29930960 | None:intergenic | 50.0% | |
| CAAGCAAGACACGACGATCA+TGG | + | chr7.1:29930945-29930964 | None:intergenic | 50.0% | |
| GATCGGTGGGAAGCTTATAG+GGG | - | chr7.1:29931538-29931557 | MS.gene87768:intron | 50.0% | |
| TTCGCCGTCACTGATGTCAA+CGG | - | chr7.1:29930880-29930899 | MS.gene87768:CDS | 50.0% | |
| CGTGTCTTGCTTGATGCTGC+TGG | - | chr7.1:29930952-29930971 | MS.gene87768:CDS | 55.0% | |
| GCAAGACACGACGATCATGG+AGG | + | chr7.1:29930942-29930961 | None:intergenic | 55.0% | |
| TGAACGTACCTTGCGACGGA+GGG | + | chr7.1:29930992-29931011 | None:intergenic | 55.0% | |
| TTGAACGTACCTTGCGACGG+AGG | + | chr7.1:29930993-29931012 | None:intergenic | 55.0% | |
| TTGCGACGGAGGGTGGTGAT+GGG | + | chr7.1:29930982-29931001 | None:intergenic | 60.0% | |
| ACGTACCTTGCGACGGAGGG+TGG | + | chr7.1:29930989-29931008 | None:intergenic | 65.0% | |
| CATCACCACCCTCCGTCGCA+AGG | - | chr7.1:29930981-29931000 | MS.gene87768:intron | 65.0% | |
| CTTGCGACGGAGGGTGGTGA+TGG | + | chr7.1:29930983-29931002 | None:intergenic | 65.0% | |
| ! | TGCGACGGAGGGTGGTGATG+GGG | + | chr7.1:29930981-29931000 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 29930859 | 29932011 | 29930859 | ID=MS.gene87768 |
| chr7.1 | mRNA | 29930859 | 29932011 | 29930859 | ID=MS.gene87768.t1;Parent=MS.gene87768 |
| chr7.1 | exon | 29931868 | 29932011 | 29931868 | ID=MS.gene87768.t1.exon1;Parent=MS.gene87768.t1 |
| chr7.1 | CDS | 29931868 | 29932011 | 29931868 | ID=cds.MS.gene87768.t1;Parent=MS.gene87768.t1 |
| chr7.1 | exon | 29931120 | 29931347 | 29931120 | ID=MS.gene87768.t1.exon2;Parent=MS.gene87768.t1 |
| chr7.1 | CDS | 29931120 | 29931347 | 29931120 | ID=cds.MS.gene87768.t1;Parent=MS.gene87768.t1 |
| chr7.1 | exon | 29930859 | 29930999 | 29930859 | ID=MS.gene87768.t1.exon3;Parent=MS.gene87768.t1 |
| chr7.1 | CDS | 29930859 | 29930999 | 29930859 | ID=cds.MS.gene87768.t1;Parent=MS.gene87768.t1 |
| Gene Sequence |
| Protein sequence |
