AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42099


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42099.t1 MTR_7g072120 94.340 212 11 1 1 211 1 212 2.19e-148 411
MS.gene42099.t1 MTR_7g072170 86.256 211 28 1 1 210 1 211 7.94e-136 380
MS.gene42099.t1 MTR_7g072140 84.434 212 32 1 1 211 1 212 1.84e-132 371
MS.gene42099.t1 MTR_7g072270 81.043 211 39 1 1 211 1 210 1.10e-129 363
MS.gene42099.t1 MTR_7g072250 70.588 221 49 4 1 210 25 240 5.90e-106 305
MS.gene42099.t1 MTR_7g072270 80.723 166 31 1 1 166 1 165 1.03e-100 288
MS.gene42099.t1 MTR_4g029430 61.458 192 68 3 19 209 12 198 5.66e-83 245
MS.gene42099.t1 MTR_7g071710 83.761 117 19 0 93 209 1 117 1.20e-72 216
MS.gene42099.t1 MTR_1g014180 54.497 189 75 6 20 203 3 185 1.85e-60 187
MS.gene42099.t1 MTR_1g014150 46.486 185 90 5 21 203 12 189 3.48e-47 154
MS.gene42099.t1 MTR_1g014160 41.579 190 85 7 32 203 59 240 4.56e-30 111
MS.gene42099.t1 MTR_5g074550 35.556 180 102 5 32 202 17 191 1.71e-26 100
MS.gene42099.t1 MTR_2g103840 30.303 198 124 6 15 208 17 204 5.05e-25 97.8
MS.gene42099.t1 MTR_4g010180 28.261 184 126 5 26 206 11 191 7.24e-23 92.0
MS.gene42099.t1 MTR_5g074560 31.214 173 109 5 32 202 20 184 5.15e-21 86.7
MS.gene42099.t1 MTR_2g103850 27.317 205 139 6 10 209 7 206 3.51e-20 85.1
MS.gene42099.t1 MTR_0650s0010 28.571 189 120 6 27 203 12 197 1.96e-18 80.1
MS.gene42099.t1 MTR_8g099000 28.723 188 114 7 35 204 45 230 1.15e-14 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42099.t1 AT5G01750 61.722 209 79 1 2 210 8 215 2.01e-102 295
MS.gene42099.t1 AT3G11740 65.285 193 65 2 21 211 2 194 1.24e-92 269
MS.gene42099.t1 AT2G14560 55.556 189 84 0 19 207 4 192 7.23e-78 232
MS.gene42099.t1 AT1G33840 53.465 202 93 1 3 204 9 209 1.21e-77 233
MS.gene42099.t1 AT5G01750 60.241 166 65 1 2 167 8 172 3.20e-77 229
MS.gene42099.t1 AT2G14560 55.556 189 84 0 19 207 64 252 9.04e-77 232
MS.gene42099.t1 AT2G14560 52.500 160 76 0 19 178 4 163 1.64e-62 192
MS.gene42099.t1 AT2G14560 52.500 160 76 0 19 178 64 223 2.62e-61 191
MS.gene42099.t1 AT1G33840 51.205 166 80 1 3 168 9 173 9.14e-61 188
MS.gene42099.t1 AT3G16900 41.935 186 87 4 22 206 15 180 7.74e-49 158
MS.gene42099.t1 AT3G56180 35.602 191 117 3 21 208 17 204 1.29e-38 132
MS.gene42099.t1 AT3G56180 35.079 191 116 4 21 208 17 202 2.28e-36 127
MS.gene42099.t1 AT3G10986 32.292 192 124 3 21 206 3 194 1.01e-31 114
MS.gene42099.t1 AT3G15810 30.508 177 117 3 35 209 33 205 2.08e-26 101
MS.gene42099.t1 AT1G80120 32.961 179 113 4 35 209 19 194 1.00e-20 86.3
MS.gene42099.t1 AT2G30270 30.000 180 112 6 25 202 12 179 7.21e-17 75.5
MS.gene42099.t1 AT2G38640 30.286 175 113 5 33 204 23 191 2.34e-16 74.3
MS.gene42099.t1 AT5G20640 24.873 197 131 5 22 203 12 206 6.75e-15 70.9
MS.gene42099.t1 AT2G05910 26.042 192 121 7 21 203 3 182 7.57e-15 70.5
MS.gene42099.t1 AT5G41590 22.120 217 154 5 3 204 2 218 2.57e-12 63.9

Find 52 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTCTTCATCTGGATTA+AGG 0.216511 7.3:+32523979 None:intergenic
AAAATCACAAACATCTTCTT+TGG 0.257604 7.3:+32523931 None:intergenic
ATGATAGGTGGGAAGCTTAT+AGG 0.292307 7.3:-32524051 MS.gene42099:CDS
TGATAGGTGGGAAGCTTATA+GGG 0.297733 7.3:-32524050 MS.gene42099:CDS
CATCTAATTTGGTCTTCATC+TGG 0.334921 7.3:+32523972 None:intergenic
GGATCTTGCCATCGTGAAAA+AGG 0.339002 7.3:-32524721 MS.gene42099:CDS
AGCTAGAAACACATCTAATT+TGG 0.366171 7.3:+32523961 None:intergenic
ACAGTATTGAGGACCGAATA+TGG 0.368124 7.3:+32524758 None:intergenic
CTTGCGACGGAGGGTGGTGA+TGG 0.396422 7.3:+32524575 None:intergenic
GAGGACCGAATATGGCGTTC+GGG 0.411432 7.3:+32524766 None:intergenic
GCAGCCCCGAACGCCATATT+CGG 0.412629 7.3:-32524771 MS.gene42099:CDS
ATCTGCATCTGCACCGATGC+AGG 0.415902 7.3:-32524793 MS.gene42099:CDS
TTCACGATGGCAAGATCCAC+TGG 0.437250 7.3:+32524726 None:intergenic
TTGCGACGGAGGGTGGTGAT+GGG 0.452287 7.3:+32524576 None:intergenic
TGAGGACCGAATATGGCGTT+CGG 0.452612 7.3:+32524765 None:intergenic
CGTGTCTTGCTTGATGCTGC+TGG 0.455360 7.3:-32524603 MS.gene42099:CDS
GCGTTCGGGGCTGCCTGCAT+CGG 0.455509 7.3:+32524780 None:intergenic
GGCGAAGTTTCCATCTGAAA+TGG 0.475827 7.3:+32524692 None:intergenic
CGATCTTGCATTGTTTATGC+TGG 0.480226 7.3:-32523884 MS.gene42099:CDS
ATGAAAGCGTAGTCAACATT+GGG 0.498681 7.3:+32523650 None:intergenic
AAATTAGATGTGTTTCTAGC+TGG 0.501483 7.3:-32523959 MS.gene42099:CDS
GCAGATGGCTGTGGTTGATA+AGG 0.510803 7.3:+32524810 None:intergenic
GATAAACCGTGACTGAGAAA+TGG 0.513280 7.3:+32523672 None:intergenic
AACATTGTATTCAAAGTGAA+AGG 0.513412 7.3:-32524651 MS.gene42099:CDS
CAAGCAAGACACGACGATCA+TGG 0.522499 7.3:+32524613 None:intergenic
TGTTGACTACGCTTTCATAG+TGG 0.527609 7.3:-32523645 MS.gene42099:CDS
ATCTAACCACATTGTTGCAC+AGG 0.530405 7.3:-32523858 MS.gene42099:intron
AGATAGTGACTATGCATGAT+AGG 0.539221 7.3:-32524066 MS.gene42099:CDS
TATGAAAGCGTAGTCAACAT+TGG 0.542595 7.3:+32523649 None:intergenic
TAAAGTCAGAGGTAGTTGGT+TGG 0.551193 7.3:-32523909 MS.gene42099:CDS
TAAAGACCATTTCTCAGTCA+CGG 0.553557 7.3:-32523678 MS.gene42099:CDS
ATGGCAAGATCCACTGGATA+CGG 0.564173 7.3:+32524732 None:intergenic
TGCGACGGAGGGTGGTGATG+GGG 0.564704 7.3:+32524577 None:intergenic
CATCACCACCCTCCGTCGCA+AGG 0.565107 7.3:-32524574 MS.gene42099:intron
TAGTGACTATGCATGATAGG+TGG 0.565567 7.3:-32524063 MS.gene42099:CDS
AGTGACTATGCATGATAGGT+GGG 0.571139 7.3:-32524062 MS.gene42099:CDS
AAGGTGATGACCATTTCAGA+TGG 0.578907 7.3:-32524702 MS.gene42099:CDS
GGTGCAGATGCAGATGGCTG+TGG 0.586855 7.3:+32524801 None:intergenic
GGATACGGAGCACAGTATTG+AGG 0.603779 7.3:+32524747 None:intergenic
TGCATCGGTGCAGATGCAGA+TGG 0.610431 7.3:+32524795 None:intergenic
TTGAACGTACCTTGCGACGG+AGG 0.612314 7.3:+32524565 None:intergenic
TGAACGTACCTTGCGACGGA+GGG 0.617417 7.3:+32524566 None:intergenic
ACGTACCTTGCGACGGAGGG+TGG 0.620243 7.3:+32524569 None:intergenic
GATAGGTGGGAAGCTTATAG+GGG 0.640463 7.3:-32524049 MS.gene42099:CDS
AGGACCGAATATGGCGTTCG+GGG 0.642653 7.3:+32524767 None:intergenic
TAATTACCTGTGCAACAATG+TGG 0.644297 7.3:+32523852 None:intergenic
ATACTGTGCTCCGTATCCAG+TGG 0.648223 7.3:-32524742 MS.gene42099:CDS
CAAGACACGACGATCATGGA+GGG 0.648557 7.3:+32524617 None:intergenic
GTTTCCGTTGACATCAGTGA+CGG 0.656884 7.3:+32524671 None:intergenic
TTCGCCGTCACTGATGTCAA+CGG 0.668229 7.3:-32524675 MS.gene42099:CDS
AAGTTGAACGTACCTTGCGA+CGG 0.684930 7.3:+32524562 None:intergenic
GCAAGACACGACGATCATGG+AGG 0.713270 7.3:+32524616 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCATTGTTTTATTTTATTT+TGG + chr7.3:32524082-32524101 None:intergenic 10.0%
!! ACAAAAATACTGAAATTATT+AGG - chr7.3:32523958-32523977 MS.gene42099:CDS 15.0%
!!! AATAATTTCAGTATTTTTGT+CGG + chr7.3:32523958-32523977 None:intergenic 15.0%
!!! TTTTAATTAATTACGTTGAT+TGG - chr7.3:32524621-32524640 MS.gene42099:CDS 15.0%
!! AAAAATAAAAATGCTCAATC+CGG + chr7.3:32523927-32523946 None:intergenic 20.0%
! AAAATAAAAATGCTCAATCC+GGG + chr7.3:32523926-32523945 None:intergenic 25.0%
! AAAATCACAAACATCTTCTT+TGG + chr7.3:32524489-32524508 None:intergenic 25.0%
! AACATTGTATTCAAAGTGAA+AGG - chr7.3:32523766-32523785 MS.gene42099:intron 25.0%
! AGTACTATATCAGTTGAAAA+CGG + chr7.3:32524309-32524328 None:intergenic 25.0%
!! ATTTAACAGTAATGTTTTCC+CGG - chr7.3:32523905-32523924 MS.gene42099:CDS 25.0%
!! TAAGCACAAATCAATTTTCA+TGG - chr7.3:32524662-32524681 MS.gene42099:CDS 25.0%
!!! ATCAACATTTTGTTTTGTGA+GGG - chr7.3:32524100-32524119 MS.gene42099:intron 25.0%
ACTATCAGCTCAATAATACA+TGG - chr7.3:32524158-32524177 MS.gene42099:intron 30.0%
AGCTAGAAACACATCTAATT+TGG + chr7.3:32524459-32524478 None:intergenic 30.0%
CTATCTGCTACACAAAATAA+TGG - chr7.3:32524046-32524065 MS.gene42099:CDS 30.0%
GGGAAAACATTACTGTTAAA+TGG + chr7.3:32523906-32523925 None:intergenic 30.0%
TACTGAAATTATTAGGTCGA+AGG - chr7.3:32523965-32523984 MS.gene42099:CDS 30.0%
TATACTCTTGTATACACAAG+AGG + chr7.3:32524596-32524615 None:intergenic 30.0%
! TACTGAATTTTGTCATGTTG+TGG - chr7.3:32524325-32524344 MS.gene42099:intron 30.0%
!! AAATTAGATGTGTTTCTAGC+TGG - chr7.3:32524458-32524477 MS.gene42099:intron 30.0%
!! CACTGTTACGAGTATTTTAA+TGG - chr7.3:32524715-32524734 MS.gene42099:CDS 30.0%
!!! ATTTTAAAGTCAGAGGTAGT+TGG - chr7.3:32524504-32524523 MS.gene42099:intron 30.0%
!!! GATCAACATTTTGTTTTGTG+AGG - chr7.3:32524099-32524118 MS.gene42099:intron 30.0%
!!! GTTTGTGATTTTAAAGTCAG+AGG - chr7.3:32524497-32524516 MS.gene42099:intron 30.0%
AGATAGTGACTATGCATGAT+AGG - chr7.3:32524351-32524370 MS.gene42099:intron 35.0%
ATCAGCTCAATAATACATGG+CGG - chr7.3:32524161-32524180 MS.gene42099:intron 35.0%
GTAACTAATACATGCTAGAG+AGG + chr7.3:32524225-32524244 None:intergenic 35.0%
TAAAGACCATTTCTCAGTCA+CGG - chr7.3:32524739-32524758 MS.gene42099:CDS 35.0%
TAATTACCTGTGCAACAATG+TGG + chr7.3:32524568-32524587 None:intergenic 35.0%
! ATGAAAGCGTAGTCAACATT+GGG + chr7.3:32524770-32524789 None:intergenic 35.0%
! CATCTAATTTGGTCTTCATC+TGG + chr7.3:32524448-32524467 None:intergenic 35.0%
! TATGAAAGCGTAGTCAACAT+TGG + chr7.3:32524771-32524790 None:intergenic 35.0%
! TTTGGTCTTCATCTGGATTA+AGG + chr7.3:32524441-32524460 None:intergenic 35.0%
AAGGTGATGACCATTTCAGA+TGG - chr7.3:32523715-32523734 MS.gene42099:CDS 40.0%
AGTGACTATGCATGATAGGT+GGG - chr7.3:32524355-32524374 MS.gene42099:intron 40.0%
ATCTAACCACATTGTTGCAC+AGG - chr7.3:32524559-32524578 MS.gene42099:intron 40.0%
ATGATAGGTGGGAAGCTTAT+AGG - chr7.3:32524366-32524385 MS.gene42099:intron 40.0%
CGATCTTGCATTGTTTATGC+TGG - chr7.3:32524533-32524552 MS.gene42099:intron 40.0%
GATAAACCGTGACTGAGAAA+TGG + chr7.3:32524748-32524767 None:intergenic 40.0%
TAAAGTCAGAGGTAGTTGGT+TGG - chr7.3:32524508-32524527 MS.gene42099:intron 40.0%
TAGTGACTATGCATGATAGG+TGG - chr7.3:32524354-32524373 MS.gene42099:intron 40.0%
TGATAGGTGGGAAGCTTATA+GGG - chr7.3:32524367-32524386 MS.gene42099:intron 40.0%
TTACTTCATTCGCTTGTCTC+AGG - chr7.3:32524241-32524260 MS.gene42099:intron 40.0%
! ACAGTATTGAGGACCGAATA+TGG + chr7.3:32523662-32523681 None:intergenic 40.0%
! TGTTGACTACGCTTTCATAG+TGG - chr7.3:32524772-32524791 MS.gene42099:CDS 40.0%
AAGTTGAACGTACCTTGCGA+CGG + chr7.3:32523858-32523877 None:intergenic 45.0%
ATGGCAAGATCCACTGGATA+CGG + chr7.3:32523688-32523707 None:intergenic 45.0%
GATAGGTGGGAAGCTTATAG+GGG - chr7.3:32524368-32524387 MS.gene42099:intron 45.0%
GGATCTTGCCATCGTGAAAA+AGG - chr7.3:32523696-32523715 MS.gene42099:CDS 45.0%
GGCGAAGTTTCCATCTGAAA+TGG + chr7.3:32523728-32523747 None:intergenic 45.0%
GTTTCCGTTGACATCAGTGA+CGG + chr7.3:32523749-32523768 None:intergenic 45.0%
!! GGTCATCACCTTTTTCACGA+TGG + chr7.3:32523707-32523726 None:intergenic 45.0%
ATACTGTGCTCCGTATCCAG+TGG - chr7.3:32523675-32523694 MS.gene42099:CDS 50.0%
CAAGACACGACGATCATGGA+GGG + chr7.3:32523803-32523822 None:intergenic 50.0%
CAAGCAAGACACGACGATCA+TGG + chr7.3:32523807-32523826 None:intergenic 50.0%
GGATACGGAGCACAGTATTG+AGG + chr7.3:32523673-32523692 None:intergenic 50.0%
TGAGGACCGAATATGGCGTT+CGG + chr7.3:32523655-32523674 None:intergenic 50.0%
TTCACGATGGCAAGATCCAC+TGG + chr7.3:32523694-32523713 None:intergenic 50.0%
TTCGCCGTCACTGATGTCAA+CGG - chr7.3:32523742-32523761 MS.gene42099:intron 50.0%
! GCAGATGGCTGTGGTTGATA+AGG + chr7.3:32523610-32523629 None:intergenic 50.0%
AGGACCGAATATGGCGTTCG+GGG + chr7.3:32523653-32523672 None:intergenic 55.0%
CGTGTCTTGCTTGATGCTGC+TGG - chr7.3:32523814-32523833 MS.gene42099:intron 55.0%
GAGGACCGAATATGGCGTTC+GGG + chr7.3:32523654-32523673 None:intergenic 55.0%
GCAAGACACGACGATCATGG+AGG + chr7.3:32523804-32523823 None:intergenic 55.0%
TGAACGTACCTTGCGACGGA+GGG + chr7.3:32523854-32523873 None:intergenic 55.0%
TTGAACGTACCTTGCGACGG+AGG + chr7.3:32523855-32523874 None:intergenic 55.0%
! ATCTGCATCTGCACCGATGC+AGG - chr7.3:32523624-32523643 MS.gene42099:CDS 55.0%
! TGCATCGGTGCAGATGCAGA+TGG + chr7.3:32523625-32523644 None:intergenic 55.0%
GCAGCCCCGAACGCCATATT+CGG - chr7.3:32523646-32523665 MS.gene42099:CDS 60.0%
GGTGCAGATGCAGATGGCTG+TGG + chr7.3:32523619-32523638 None:intergenic 60.0%
TTGCGACGGAGGGTGGTGAT+GGG + chr7.3:32523844-32523863 None:intergenic 60.0%
ACGTACCTTGCGACGGAGGG+TGG + chr7.3:32523851-32523870 None:intergenic 65.0%
CATCACCACCCTCCGTCGCA+AGG - chr7.3:32523843-32523862 MS.gene42099:intron 65.0%
CTTGCGACGGAGGGTGGTGA+TGG + chr7.3:32523845-32523864 None:intergenic 65.0%
! TGCGACGGAGGGTGGTGATG+GGG + chr7.3:32523843-32523862 None:intergenic 65.0%
GCGTTCGGGGCTGCCTGCAT+CGG + chr7.3:32523640-32523659 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 32523598 32524841 32523598 ID=MS.gene42099
chr7.3 mRNA 32523598 32524841 32523598 ID=MS.gene42099.t1;Parent=MS.gene42099
chr7.3 exon 32524575 32524841 32524575 ID=MS.gene42099.t1.exon1;Parent=MS.gene42099.t1
chr7.3 CDS 32524575 32524841 32524575 ID=cds.MS.gene42099.t1;Parent=MS.gene42099.t1
chr7.3 exon 32523859 32524086 32523859 ID=MS.gene42099.t1.exon2;Parent=MS.gene42099.t1
chr7.3 CDS 32523859 32524086 32523859 ID=cds.MS.gene42099.t1;Parent=MS.gene42099.t1
chr7.3 exon 32523598 32523738 32523598 ID=MS.gene42099.t1.exon3;Parent=MS.gene42099.t1
chr7.3 CDS 32523598 32523738 32523598 ID=cds.MS.gene42099.t1;Parent=MS.gene42099.t1
Gene Sequence

>MS.gene42099

ATGGCTTATCCTTATCAACCACAGCCATCTGCATCTGCACCGATGCAGGCAGCCCCGAACGCCATATTCGGTCCTCAATACTGTGCTCCGTATCCAGTGGATCTTGCCATCGTGAAAAAGGTGATGACCATTTCAGATGGAAACTTCGCCGTCACTGATGTCAACGGAAACATTGTATTCAAAGTGAAAGGTTCTCTTTTAACCCTCCATGATCGTCGTGTCTTGCTTGATGCTGCTGGTAACCCCATCACCACCCTCCGTCGCAAGGTACGTTCAACTTTATTCTCTCTTTTACGAATTTTCTTCCATTTAACAGTAATGTTTTCCCGGATTGAGCATTTTTATTTTTTAAATCAACCGACAAAAATACTGAAATTATTAGGTCGAAGGTGATGATCTGAGTTTGAATCTCGAATCAACTTTTCAATTGTTATTATTGTTATTTCGTCTATCTGCTACACAAAATAATGGTCTCATTGTACCAAAATAAAATAAAACAATGATCAACATTTTGTTTTGTGAGGGAAACAAAGCTTACAACATTTCATTAGATATTTATTACTATCAGCTCAATAATACATGGCGGTACATCAACAGAAATATGAAGAGTAGTTAAGAATGTGACCTCTCTAGCATGTATTAGTTACTTCATTCGCTTGTCTCAGGCTAAACTCGACATGAGAGTTTGTGAAAAATAAATTACAACATCCGTTTTCAACTGATATAGTACTGAATTTTGTCATGTTGTGGTGCAGATAGTGACTATGCATGATAGGTGGGAAGCTTATAGGGGTGAAAGCACAGATTCTAAAGATCTAATATTTACATTGAAGAGATCATCCTTAATCCAGATGAAGACCAAATTAGATGTGTTTCTAGCTGGTAATACCAAAGAAGATGTTTGTGATTTTAAAGTCAGAGGTAGTTGGTTGGAACGATCTTGCATTGTTTATGCTGGTGAATCTAACCACATTGTTGCACAGGTAATTAAAAATCCTCTTGTGTATACAAGAGTATATTTAGTTTTAATTAATTACGTTGATTGGATTATAATAAGTATATACTAAGCACAAATCAATTTTCATGGCATTGTTGTTATGCAGATGCACAAAAAGCACACTGTTACGAGTATTTTAATGGATAAAGACCATTTCTCAGTCACGGTTTATCCCAATGTTGACTACGCTTTCATAGTGGCATTAATCGTGATTCTTGATGAGACTAACGAAGATGAAAAAGATTGA

Protein sequence

>MS.gene42099.t1

MAYPYQPQPSASAPMQAAPNAIFGPQYCAPYPVDLAIVKKVMTISDGNFAVTDVNGNIVFKVKGSLLTLHDRRVLLDAAGNPITTLRRKIVTMHDRWEAYRGESTDSKDLIFTLKRSSLIQMKTKLDVFLAGNTKEDVCDFKVRGSWLERSCIVYAGESNHIVAQMHKKHTVTSILMDKDHFSVTVYPNVDYAFIVALIVILDETNEDEKD