AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42103


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42103.t1 MTR_7g072120 82.381 210 29 4 1 202 1 210 7.89e-128 358
MS.gene42103.t1 MTR_7g072170 80.000 210 34 2 1 202 1 210 1.32e-124 351
MS.gene42103.t1 MTR_7g072270 79.227 207 37 1 1 201 1 207 2.67e-124 350
MS.gene42103.t1 MTR_7g072140 78.571 210 37 2 1 202 1 210 1.36e-122 345
MS.gene42103.t1 MTR_7g072250 67.281 217 50 3 1 200 25 237 1.73e-100 291
MS.gene42103.t1 MTR_7g072270 78.182 165 30 1 1 159 1 165 2.56e-96 277
MS.gene42103.t1 MTR_7g071710 95.763 118 5 0 86 203 1 118 1.15e-81 238
MS.gene42103.t1 MTR_4g029430 56.345 197 81 2 6 201 5 197 1.21e-79 236
MS.gene42103.t1 MTR_1g014180 51.613 186 80 5 16 196 5 185 1.11e-58 182
MS.gene42103.t1 MTR_1g014150 46.196 184 91 4 14 195 11 188 5.14e-47 153
MS.gene42103.t1 MTR_1g014160 39.894 188 88 6 26 195 59 239 4.99e-30 111
MS.gene42103.t1 MTR_2g103840 30.539 167 108 5 39 201 42 204 6.57e-23 92.0
MS.gene42103.t1 MTR_5g074550 30.556 180 110 6 26 195 17 191 4.22e-21 86.7
MS.gene42103.t1 MTR_4g010180 27.273 187 129 6 20 202 11 194 1.21e-20 85.9
MS.gene42103.t1 MTR_2g103850 29.167 168 111 5 39 202 43 206 1.52e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42103.t1 AT5G01750 57.000 200 85 1 4 202 15 214 6.78e-92 268
MS.gene42103.t1 AT3G11740 58.421 190 77 2 15 202 2 191 1.47e-82 243
MS.gene42103.t1 AT2G14560 51.295 193 92 2 9 200 1 192 2.62e-71 215
MS.gene42103.t1 AT2G14560 50.510 196 95 2 6 200 58 252 2.53e-70 215
MS.gene42103.t1 AT1G33840 46.445 211 101 2 1 199 1 211 6.83e-68 207
MS.gene42103.t1 AT5G01750 55.063 158 70 1 4 160 15 172 6.33e-67 203
MS.gene42103.t1 AT2G14560 48.171 164 83 2 9 171 1 163 2.60e-55 174
MS.gene42103.t1 AT2G14560 49.045 157 78 2 6 161 58 213 2.21e-54 173
MS.gene42103.t1 AT3G16900 43.243 185 85 4 16 199 15 180 6.46e-50 160
MS.gene42103.t1 AT1G33840 42.775 173 87 2 1 161 1 173 6.87e-50 160
MS.gene42103.t1 AT3G56180 35.052 194 119 4 12 201 14 204 1.40e-36 127
MS.gene42103.t1 AT3G56180 34.536 194 118 5 12 201 14 202 3.18e-34 120
MS.gene42103.t1 AT3G10986 32.632 190 121 4 15 197 3 192 6.85e-30 109
MS.gene42103.t1 AT3G15810 32.143 168 104 6 39 202 44 205 2.11e-24 95.9
MS.gene42103.t1 AT1G80120 32.738 168 106 4 39 202 30 194 1.38e-19 83.2
MS.gene42103.t1 AT2G38640 32.544 169 105 5 33 198 30 192 4.15e-17 76.3
MS.gene42103.t1 AT5G20640 28.729 181 112 5 36 201 33 211 1.87e-15 72.4
MS.gene42103.t1 AT2G30270 28.902 173 108 7 26 195 19 179 4.87e-15 70.5
MS.gene42103.t1 AT2G05910 28.402 169 100 7 39 198 28 184 7.06e-14 67.4
MS.gene42103.t1 AT5G41590 24.432 176 123 4 33 198 44 219 2.95e-11 60.8

Find 51 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACAAACATCTTCTT+TGG 0.257604 7.3:+32619857 None:intergenic
GGATTTGGCGGTCGTGAAAA+AGG 0.290385 7.3:-32620768 MS.gene42103:CDS
TGCTCCCTATCCGGTGGATT+TGG 0.306791 7.3:-32620783 MS.gene42103:CDS
GCACCCACCACCACCATATT+CGG 0.362096 7.3:-32620818 MS.gene42103:CDS
ACAATATTGAGAGCCGAATA+TGG 0.363340 7.3:+32620805 None:intergenic
ATTGCCATTGACATCTTTAA+CGG 0.374139 7.3:+32620721 None:intergenic
TAGGGTGGTGATGGGGTTAC+CGG 0.377318 7.3:+32620634 None:intergenic
GTGGTGGTGGGTGCCGGCAT+CGG 0.380802 7.3:+32620827 None:intergenic
CCTCCGTGACCGCCGTGTCT+TGG 0.425443 7.3:-32620666 MS.gene42103:CDS
TCAATATTGTGCTCCCTATC+CGG 0.428008 7.3:-32620792 MS.gene42103:CDS
CTTGCGACGTAGGGTGGTGA+TGG 0.430403 7.3:+32620625 None:intergenic
GCCGGCATCGGTGGTTGATA+AGG 0.437331 7.3:+32620839 None:intergenic
AATATGGTGGTGGTGGGTGC+CGG 0.438186 7.3:+32620821 None:intergenic
GTGGTGGGTGCCGGCATCGG+TGG 0.452113 7.3:+32620830 None:intergenic
GATAAACAGTGACCGCAAAA+TGG 0.467602 7.3:+32619601 None:intergenic
TTCACGACCGCCAAATCCAC+CGG 0.471320 7.3:+32620773 None:intergenic
TTGCGACGTAGGGTGGTGAT+GGG 0.485246 7.3:+32620626 None:intergenic
ACCGCCAAATCCACCGGATA+GGG 0.489401 7.3:+32620779 None:intergenic
TCCTTATCAACCACCGATGC+CGG 0.493777 7.3:-32620840 MS.gene42103:CDS
CGTGTCTTGGTTGATGCCTC+CGG 0.494853 7.3:-32620653 MS.gene42103:CDS
ATGAAAGCGTAATCAACATT+GGG 0.498374 7.3:+32619579 None:intergenic
TAAAGTCAAAGGTAGTTGGT+TGG 0.499063 7.3:-32619835 MS.gene42103:CDS
TCCCTATCCGGTGGATTTGG+CGG 0.505154 7.3:-32620780 MS.gene42103:CDS
TATGAAAGCGTAATCAACAT+TGG 0.519673 7.3:+32619578 None:intergenic
CAACCAAGACACGGCGGTCA+CGG 0.526860 7.3:+32620663 None:intergenic
AACGGTGAAGTTGTCTGCCA+TGG 0.529412 7.3:+32620739 None:intergenic
TTGTACGTACCTTGCGACGT+AGG 0.529527 7.3:+32620615 None:intergenic
TACTAACAACATTGTTGCAC+AGG 0.530405 7.3:-32619784 MS.gene42103:intron
TGTTGATTACGCTTTCATAG+TGG 0.534696 7.3:-32619574 MS.gene42103:CDS
TGTACGTACCTTGCGACGTA+GGG 0.547525 7.3:+32620616 None:intergenic
AGAGCCGAATATGGTGGTGG+TGG 0.571753 7.3:+32620814 None:intergenic
AATGTCGTGTTTAAAGTGAA+AGG 0.575214 7.3:-32620701 MS.gene42103:CDS
CGATCTTGTATTATTTACGC+TGG 0.576867 7.3:-32619810 MS.gene42103:CDS
AGTGACTATGCATGATCGGT+GGG 0.580863 7.3:-32619988 MS.gene42103:CDS
GACCGCCAAATCCACCGGAT+AGG 0.585193 7.3:+32620778 None:intergenic
CATCACCACCCTACGTCGCA+AGG 0.590882 7.3:-32620624 MS.gene42103:intron
TTCACCGTTAAAGATGTCAA+TGG 0.593126 7.3:-32620725 MS.gene42103:CDS
CCAAGACACGGCGGTCACGG+AGG 0.598529 7.3:+32620666 None:intergenic
CAAGACACGGCGGTCACGGA+GGG 0.598937 7.3:+32620667 None:intergenic
TAGTGACTATGCATGATCGG+TGG 0.614019 7.3:-32619989 MS.gene42103:CDS
GGTGGTGATGGGGTTACCGG+AGG 0.620164 7.3:+32620637 None:intergenic
ATATTGTGCTCCCTATCCGG+TGG 0.628631 7.3:-32620789 MS.gene42103:CDS
AGATAGTGACTATGCATGAT+CGG 0.644746 7.3:-32619992 MS.gene42103:CDS
TGCGACGTAGGGTGGTGATG+GGG 0.652061 7.3:+32620627 None:intergenic
CGGAGGCATCAACCAAGACA+CGG 0.654823 7.3:+32620654 None:intergenic
TTGAGAGCCGAATATGGTGG+TGG 0.662726 7.3:+32620811 None:intergenic
ACGTACCTTGCGACGTAGGG+TGG 0.669594 7.3:+32620619 None:intergenic
GAGCCGAATATGGTGGTGGT+GGG 0.669874 7.3:+32620815 None:intergenic
GATCGGTGGGAAGCTTACAG+AGG 0.712766 7.3:-32619975 MS.gene42103:CDS
ATATTGAGAGCCGAATATGG+TGG 0.741009 7.3:+32620808 None:intergenic
AGGCATCAACCAAGACACGG+CGG 0.796971 7.3:+32620657 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTTTTTACTTTATTTTT+GGG + chr7.3:32620261-32620280 None:intergenic 10.0%
!!! TATTTTTTAAAATAAAGAGT+AGG - chr7.3:32619873-32619892 MS.gene42103:CDS 10.0%
!!! TTAAGTTTTTACTTTATTTT+TGG + chr7.3:32620262-32620281 None:intergenic 10.0%
!!! TGTATATACAAAAGTATATT+AGG - chr7.3:32620633-32620652 MS.gene42103:CDS 15.0%
!!! AAAAAAATAGTTTTGCTCAA+TGG - chr7.3:32620197-32620216 MS.gene42103:intron 20.0%
!!! ATATTAGGTTTTACTTTGAT+TGG - chr7.3:32620648-32620667 MS.gene42103:CDS 20.0%
! AAAATCACAAACATCTTCTT+TGG + chr7.3:32620524-32620543 None:intergenic 25.0%
! ACACTTTATTGAGTTATATG+AGG + chr7.3:32620332-32620351 None:intergenic 25.0%
! AGTGTGTGAATTTCAATAAT+TGG - chr7.3:32620226-32620245 MS.gene42103:intron 25.0%
! TTGAGTTATATGAGGTATAA+TGG + chr7.3:32620324-32620343 None:intergenic 25.0%
! TTTGATTGGATTATCACTTA+AGG - chr7.3:32620662-32620681 MS.gene42103:CDS 25.0%
!! AATTGATAAAGACCATTTTG+CGG - chr7.3:32620765-32620784 MS.gene42103:CDS 25.0%
!!! ATTTTAAAGTCAAAGGTAGT+TGG - chr7.3:32620539-32620558 MS.gene42103:intron 25.0%
!!! GTTTGTGATTTTAAAGTCAA+AGG - chr7.3:32620532-32620551 MS.gene42103:intron 25.0%
ATTGCCATTGACATCTTTAA+CGG + chr7.3:32619660-32619679 None:intergenic 30.0%
! AATGTCGTGTTTAAAGTGAA+AGG - chr7.3:32619677-32619696 MS.gene42103:intron 30.0%
! ATGAAAGCGTAATCAACATT+GGG + chr7.3:32620802-32620821 None:intergenic 30.0%
! CAAACTAGATGTTTTTCTAG+CGG - chr7.3:32620492-32620511 MS.gene42103:intron 30.0%
! GATTCATCAAGTTGTACTAT+TGG - chr7.3:32620360-32620379 MS.gene42103:intron 30.0%
! TATGAAAGCGTAATCAACAT+TGG + chr7.3:32620803-32620822 None:intergenic 30.0%
!! AAACTAGATGTTTTTCTAGC+GGG - chr7.3:32620493-32620512 MS.gene42103:intron 30.0%
ACAATATTGAGAGCCGAATA+TGG + chr7.3:32619576-32619595 None:intergenic 35.0%
AGATAGTGACTATGCATGAT+CGG - chr7.3:32620386-32620405 MS.gene42103:intron 35.0%
CGATCTTGTATTATTTACGC+TGG - chr7.3:32620568-32620587 MS.gene42103:intron 35.0%
CGCTAGAAAAACATCTAGTT+TGG + chr7.3:32620494-32620513 None:intergenic 35.0%
TAAAGTCAAAGGTAGTTGGT+TGG - chr7.3:32620543-32620562 MS.gene42103:intron 35.0%
TACTAACAACATTGTTGCAC+AGG - chr7.3:32620594-32620613 MS.gene42103:intron 35.0%
TTCACCGTTAAAGATGTCAA+TGG - chr7.3:32619653-32619672 MS.gene42103:intron 35.0%
! TGTTGATTACGCTTTCATAG+TGG - chr7.3:32620804-32620823 MS.gene42103:CDS 35.0%
ATATTGAGAGCCGAATATGG+TGG + chr7.3:32619573-32619592 None:intergenic 40.0%
CGTGAAAAAGGTAATGACCA+TGG - chr7.3:32619622-32619641 MS.gene42103:CDS 40.0%
GATAAACAGTGACCGCAAAA+TGG + chr7.3:32620780-32620799 None:intergenic 40.0%
TCAATATTGTGCTCCCTATC+CGG - chr7.3:32619586-32619605 MS.gene42103:CDS 40.0%
AAGAGTAGGACATCCAAGTG+TGG - chr7.3:32619887-32619906 MS.gene42103:CDS 45.0%
AGTGACTATGCATGATCGGT+GGG - chr7.3:32620390-32620409 MS.gene42103:intron 45.0%
TAGTGACTATGCATGATCGG+TGG - chr7.3:32620389-32620408 MS.gene42103:intron 45.0%
AACGGTGAAGTTGTCTGCCA+TGG + chr7.3:32619642-32619661 None:intergenic 50.0%
ATATTGTGCTCCCTATCCGG+TGG - chr7.3:32619589-32619608 MS.gene42103:CDS 50.0%
GGATTTGGCGGTCGTGAAAA+AGG - chr7.3:32619610-32619629 MS.gene42103:CDS 50.0%
TCCTTATCAACCACCGATGC+CGG - chr7.3:32619538-32619557 MS.gene42103:CDS 50.0%
TGTACGTACCTTGCGACGTA+GGG + chr7.3:32619765-32619784 None:intergenic 50.0%
TTGAGAGCCGAATATGGTGG+TGG + chr7.3:32619570-32619589 None:intergenic 50.0%
TTGTACGTACCTTGCGACGT+AGG + chr7.3:32619766-32619785 None:intergenic 50.0%
ACCGCCAAATCCACCGGATA+GGG + chr7.3:32619602-32619621 None:intergenic 55.0%
AGGCATCAACCAAGACACGG+CGG + chr7.3:32619724-32619743 None:intergenic 55.0%
CAACCACGTGAGTCCACACT+TGG + chr7.3:32619903-32619922 None:intergenic 55.0%
CATCCAAGTGTGGACTCACG+TGG - chr7.3:32619897-32619916 MS.gene42103:CDS 55.0%
GATCGGTGGGAAGCTTACAG+AGG - chr7.3:32620403-32620422 MS.gene42103:intron 55.0%
GCACCCACCACCACCATATT+CGG - chr7.3:32619560-32619579 MS.gene42103:CDS 55.0%
TTCACGACCGCCAAATCCAC+CGG + chr7.3:32619608-32619627 None:intergenic 55.0%
TTGCGACGTAGGGTGGTGAT+GGG + chr7.3:32619755-32619774 None:intergenic 55.0%
! CGTGTCTTGGTTGATGCCTC+CGG - chr7.3:32619725-32619744 MS.gene42103:intron 55.0%
! TCCCTATCCGGTGGATTTGG+CGG - chr7.3:32619598-32619617 MS.gene42103:CDS 55.0%
! TGCTCCCTATCCGGTGGATT+TGG - chr7.3:32619595-32619614 MS.gene42103:CDS 55.0%
!! AATATGGTGGTGGTGGGTGC+CGG + chr7.3:32619560-32619579 None:intergenic 55.0%
!! AGAGCCGAATATGGTGGTGG+TGG + chr7.3:32619567-32619586 None:intergenic 55.0%
!! CGGAGGCATCAACCAAGACA+CGG + chr7.3:32619727-32619746 None:intergenic 55.0%
!! GAGCCGAATATGGTGGTGGT+GGG + chr7.3:32619566-32619585 None:intergenic 55.0%
!! TAGGGTGGTGATGGGGTTAC+CGG + chr7.3:32619747-32619766 None:intergenic 55.0%
ACGTACCTTGCGACGTAGGG+TGG + chr7.3:32619762-32619781 None:intergenic 60.0%
CAACCAAGACACGGCGGTCA+CGG + chr7.3:32619718-32619737 None:intergenic 60.0%
CATCACCACCCTACGTCGCA+AGG - chr7.3:32619754-32619773 MS.gene42103:intron 60.0%
CTTGCGACGTAGGGTGGTGA+TGG + chr7.3:32619756-32619775 None:intergenic 60.0%
GACCGCCAAATCCACCGGAT+AGG + chr7.3:32619603-32619622 None:intergenic 60.0%
! TGCGACGTAGGGTGGTGATG+GGG + chr7.3:32619754-32619773 None:intergenic 60.0%
!! GCCGGCATCGGTGGTTGATA+AGG + chr7.3:32619542-32619561 None:intergenic 60.0%
CAAGACACGGCGGTCACGGA+GGG + chr7.3:32619714-32619733 None:intergenic 65.0%
!! GGTGGTGATGGGGTTACCGG+AGG + chr7.3:32619744-32619763 None:intergenic 65.0%
CCAAGACACGGCGGTCACGG+AGG + chr7.3:32619715-32619734 None:intergenic 70.0%
CCTCCGTGACCGCCGTGTCT+TGG - chr7.3:32619712-32619731 MS.gene42103:intron 70.0%
!! GTGGTGGTGGGTGCCGGCAT+CGG + chr7.3:32619554-32619573 None:intergenic 70.0%
!! GTGGTGGGTGCCGGCATCGG+TGG + chr7.3:32619551-32619570 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 32619530 32620870 32619530 ID=MS.gene42103
chr7.3 mRNA 32619530 32620870 32619530 ID=MS.gene42103.t1;Parent=MS.gene42103
chr7.3 exon 32620625 32620870 32620625 ID=MS.gene42103.t1.exon1;Parent=MS.gene42103.t1
chr7.3 CDS 32620625 32620870 32620625 ID=cds.MS.gene42103.t1;Parent=MS.gene42103.t1
chr7.3 exon 32619785 32620012 32619785 ID=MS.gene42103.t1.exon2;Parent=MS.gene42103.t1
chr7.3 CDS 32619785 32620012 32619785 ID=cds.MS.gene42103.t1;Parent=MS.gene42103.t1
chr7.3 exon 32619530 32619667 32619530 ID=MS.gene42103.t1.exon3;Parent=MS.gene42103.t1
chr7.3 CDS 32619530 32619667 32619530 ID=cds.MS.gene42103.t1;Parent=MS.gene42103.t1
Gene Sequence

>MS.gene42103

ATGGCTTATCCTTATCAACCACCGATGCCGGCACCCACCACCACCATATTCGGCTCTCAATATTGTGCTCCCTATCCGGTGGATTTGGCGGTCGTGAAAAAGGTAATGACCATGGCAGACAACTTCACCGTTAAAGATGTCAATGGCAATGTCGTGTTTAAAGTGAAAGGTTCTCTCTTAACCCTCCGTGACCGCCGTGTCTTGGTTGATGCCTCCGGTAACCCCATCACCACCCTACGTCGCAAGGTACGTACAACTTTATTAAGAAACTTTGCTTTCATATTTTTATTTTAATGCTCAATATTTTAATTTTGTGATGATTTTTTCTACATATATGATATTTTATTTTTTAAAATAAAGAGTAGGACATCCAAGTGTGGACTCACGTGGTTGCACGTTGAGCATTATCTTTAGATAAGACGTAATTTTATTACATTTAATAATATTTAATATGAAAAATTGTATGTGTTGTAAAATTATATATTTTGTAACTTGAAACACTTTATATCAAAGTTTGTAAGTTAAAAATTATGCAATTTAAATTTAGCTGATGATTAATTTTAGTAATCTTTTCAAAGAGAAATATGATCTATTTTACTCATCATACGAGCTCATATATAAGTTATTCACTTTCTGCAGAAAAAAAATAGTTATTCACTATCAACTAAAAAAAATAGTTTTGCTCAATGGAGTCTTAGTGTGTGAATTTCAATAATTGGTTGATGAAACCCAAAAATAAAGTAAAAACTTAAATGTTAGAGTAATGTTTATTGTTAAAAAATTCATATTATCCATTATACCTCATATAACTCAATAAAGTGTTGTTTAATGATTCATCAAGTTGTACTATTGGTGCAGATAGTGACTATGCATGATCGGTGGGAAGCTTACAGAGGAGAAAGCACAAATTCGAAAGATCTTATATTTACATTGAGAAAATCATCGCTATTGCAGTTGAAGACCAAACTAGATGTTTTTCTAGCGGGTAATACCAAAGAAGATGTTTGTGATTTTAAAGTCAAAGGTAGTTGGTTGGAACGATCTTGTATTATTTACGCTGGTGATACTAACAACATTGTTGCACAGGTAATTAAAAAGCATCTTGTATATACAAAAGTATATTAGGTTTTACTTTGATTGGATTATCACTTAAGGAAATATACTAATCATAAATCAATTTTTGTGATATTGTTGTTACTGCAGATGCACAAAAAGCACACTATTCAAAGTATTTTAATTGATAAAGACCATTTTGCGGTCACTGTTTATCCCAATGTTGATTACGCTTTCATAGTGGCATTAATCGCGATTCTTAATGAGATTAACGAAGATGATGATTGA

Protein sequence

>MS.gene42103.t1

MAYPYQPPMPAPTTTIFGSQYCAPYPVDLAVVKKVMTMADNFTVKDVNGNVVFKVKGSLLTLRDRRVLVDASGNPITTLRRKIVTMHDRWEAYRGESTNSKDLIFTLRKSSLLQLKTKLDVFLAGNTKEDVCDFKVKGSWLERSCIIYAGDTNNIVAQMHKKHTIQSILIDKDHFAVTVYPNVDYAFIVALIAILNEINEDDD