Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42100.t1 | XP_004492957.1 | 66.4 | 211 | 63 | 2 | 1 | 203 | 1 | 211 | 1.60E-70 | 275.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42100.t1 | Q9LZX1 | 48.3 | 207 | 102 | 3 | 2 | 203 | 8 | 214 | 2.5e-51 | 203.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42100.t1 | A0A1S2XRT7 | 66.4 | 211 | 63 | 2 | 1 | 203 | 1 | 211 | 1.2e-70 | 275.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene42100 | MS.gene42096 | PPI |
| MS.gene42100 | MS.gene87038 | PPI |
| MS.gene91237 | MS.gene42100 | PPI |
| MS.gene87041 | MS.gene42100 | PPI |
| MS.gene26305 | MS.gene42100 | PPI |
| MS.gene009719 | MS.gene42100 | PPI |
| MS.gene42100 | MS.gene42099 | PPI |
| MS.gene42100 | MS.gene91238 | PPI |
| MS.gene42103 | MS.gene42100 | PPI |
| MS.gene42098 | MS.gene42100 | PPI |
| MS.gene42100 | MS.gene87040 | PPI |
| MS.gene42100 | MS.gene25045 | PPI |
| MS.gene87769 | MS.gene42100 | PPI |
| MS.gene42100 | MS.gene87046 | PPI |
| MS.gene42100 | MS.gene87768 | PPI |
| MS.gene42100 | MS.gene81036 | PPI |
| MS.gene87043 | MS.gene42100 | PPI |
| MS.gene99704 | MS.gene42100 | PPI |
| MS.gene051028 | MS.gene42100 | PPI |
| MS.gene87767 | MS.gene42100 | PPI |
| MS.gene87039 | MS.gene42100 | PPI |
| MS.gene42100 | MS.gene009718 | PPI |
| MS.gene42848 | MS.gene42100 | PPI |
| MS.gene42099 | MS.gene42100 | PPI |
| MS.gene42091 | MS.gene42100 | PPI |
| MS.gene42100 | MS.gene42097 | PPI |
| MS.gene42100 | MS.gene87762 | PPI |
| MS.gene87040 | MS.gene42100 | PPI |
| MS.gene87765 | MS.gene42100 | PPI |
| MS.gene99705 | MS.gene42100 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42100.t1 | MTR_7g072120 | 81.905 | 210 | 31 | 3 | 1 | 203 | 1 | 210 | 1.17e-127 | 358 |
| MS.gene42100.t1 | MTR_7g072270 | 79.327 | 208 | 36 | 2 | 1 | 202 | 1 | 207 | 6.53e-124 | 349 |
| MS.gene42100.t1 | MTR_7g072170 | 79.621 | 211 | 34 | 3 | 1 | 203 | 1 | 210 | 4.64e-123 | 347 |
| MS.gene42100.t1 | MTR_7g072140 | 78.199 | 211 | 37 | 3 | 1 | 203 | 1 | 210 | 1.04e-120 | 341 |
| MS.gene42100.t1 | MTR_7g072250 | 67.431 | 218 | 49 | 4 | 1 | 201 | 25 | 237 | 2.60e-99 | 288 |
| MS.gene42100.t1 | MTR_7g072270 | 78.313 | 166 | 29 | 2 | 1 | 160 | 1 | 165 | 8.06e-96 | 276 |
| MS.gene42100.t1 | MTR_7g071710 | 96.610 | 118 | 4 | 0 | 87 | 204 | 1 | 118 | 5.41e-82 | 239 |
| MS.gene42100.t1 | MTR_4g029430 | 57.216 | 194 | 78 | 2 | 10 | 202 | 8 | 197 | 2.20e-79 | 236 |
| MS.gene42100.t1 | MTR_1g014180 | 51.613 | 186 | 80 | 5 | 17 | 197 | 5 | 185 | 2.14e-58 | 182 |
| MS.gene42100.t1 | MTR_1g014150 | 45.263 | 190 | 96 | 4 | 9 | 196 | 5 | 188 | 7.87e-48 | 155 |
| MS.gene42100.t1 | MTR_1g014160 | 39.894 | 188 | 88 | 6 | 27 | 196 | 59 | 239 | 7.46e-30 | 110 |
| MS.gene42100.t1 | MTR_2g103840 | 31.138 | 167 | 107 | 5 | 40 | 202 | 42 | 204 | 6.54e-23 | 92.0 |
| MS.gene42100.t1 | MTR_5g074550 | 31.111 | 180 | 109 | 6 | 27 | 196 | 17 | 191 | 1.94e-21 | 87.8 |
| MS.gene42100.t1 | MTR_4g010180 | 27.545 | 167 | 115 | 5 | 40 | 203 | 31 | 194 | 3.19e-20 | 84.7 |
| MS.gene42100.t1 | MTR_2g103850 | 29.762 | 168 | 110 | 5 | 40 | 203 | 43 | 206 | 1.23e-19 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42100.t1 | AT5G01750 | 57.005 | 207 | 84 | 3 | 2 | 203 | 8 | 214 | 8.36e-91 | 265 |
| MS.gene42100.t1 | AT3G11740 | 58.421 | 190 | 77 | 2 | 16 | 203 | 2 | 191 | 1.68e-82 | 243 |
| MS.gene42100.t1 | AT2G14560 | 52.151 | 186 | 88 | 1 | 17 | 201 | 7 | 192 | 3.42e-71 | 215 |
| MS.gene42100.t1 | AT2G14560 | 50.254 | 197 | 95 | 2 | 6 | 201 | 58 | 252 | 4.63e-70 | 214 |
| MS.gene42100.t1 | AT1G33840 | 46.445 | 211 | 102 | 2 | 1 | 200 | 1 | 211 | 6.49e-68 | 207 |
| MS.gene42100.t1 | AT5G01750 | 55.152 | 165 | 69 | 3 | 2 | 161 | 8 | 172 | 7.59e-66 | 200 |
| MS.gene42100.t1 | AT2G14560 | 48.428 | 159 | 81 | 1 | 15 | 172 | 5 | 163 | 3.39e-55 | 174 |
| MS.gene42100.t1 | AT2G14560 | 48.734 | 158 | 78 | 2 | 6 | 162 | 58 | 213 | 4.03e-54 | 173 |
| MS.gene42100.t1 | AT3G16900 | 41.327 | 196 | 95 | 4 | 6 | 200 | 4 | 180 | 1.38e-50 | 162 |
| MS.gene42100.t1 | AT1G33840 | 42.775 | 173 | 88 | 2 | 1 | 162 | 1 | 173 | 6.66e-50 | 160 |
| MS.gene42100.t1 | AT3G56180 | 35.602 | 191 | 116 | 4 | 16 | 202 | 17 | 204 | 1.34e-35 | 124 |
| MS.gene42100.t1 | AT3G56180 | 35.079 | 191 | 115 | 5 | 16 | 202 | 17 | 202 | 2.67e-33 | 118 |
| MS.gene42100.t1 | AT3G10986 | 32.632 | 190 | 121 | 4 | 16 | 198 | 3 | 192 | 1.42e-29 | 108 |
| MS.gene42100.t1 | AT3G15810 | 31.548 | 168 | 105 | 6 | 40 | 203 | 44 | 205 | 5.68e-24 | 95.1 |
| MS.gene42100.t1 | AT1G80120 | 33.333 | 168 | 105 | 4 | 40 | 203 | 30 | 194 | 1.71e-19 | 82.8 |
| MS.gene42100.t1 | AT2G38640 | 30.808 | 198 | 123 | 7 | 6 | 199 | 5 | 192 | 4.07e-17 | 76.3 |
| MS.gene42100.t1 | AT2G30270 | 28.902 | 173 | 108 | 7 | 27 | 196 | 19 | 179 | 2.67e-15 | 71.2 |
| MS.gene42100.t1 | AT5G20640 | 26.442 | 208 | 135 | 6 | 11 | 202 | 6 | 211 | 3.23e-15 | 71.6 |
| MS.gene42100.t1 | AT2G05910 | 29.586 | 169 | 98 | 8 | 40 | 199 | 28 | 184 | 1.34e-13 | 66.6 |
| MS.gene42100.t1 | AT5G41590 | 23.864 | 176 | 124 | 4 | 34 | 199 | 44 | 219 | 6.04e-11 | 60.1 |
Find 49 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCACAAACATCTTCTT+TGG | 0.257604 | 7.3:+32578135 | None:intergenic |
| GGATTTGGCGGTCGTGAAAA+AGG | 0.290385 | 7.3:-32585135 | MS.gene42100:CDS |
| TGCTCCTTATCCGGTGGATT+TGG | 0.297420 | 7.3:-32585150 | MS.gene42100:CDS |
| GTTGCCATTGACATCTTTAA+CGG | 0.359778 | 7.3:+32585088 | None:intergenic |
| GTGGTGGTGGGTGCCGGCAT+CGG | 0.363224 | 7.3:+32585197 | None:intergenic |
| ACAATATTGAGAGCCGAATA+TGG | 0.363340 | 7.3:+32585172 | None:intergenic |
| GCCGAATATGGTGGTGGTGG+TGG | 0.392837 | 7.3:+32585184 | None:intergenic |
| TCAATATTGTGCTCCTTATC+CGG | 0.417584 | 7.3:-32585159 | MS.gene42100:CDS |
| CCTCCGTGACCGCCGTGTCT+TGG | 0.425443 | 7.3:-32585033 | MS.gene42100:CDS |
| GCCGGCATCGGTGGTTGATA+AGG | 0.437331 | 7.3:+32585209 | None:intergenic |
| CTTGCGACGTAGGGTGGTGA+TGG | 0.443247 | 7.3:+32584992 | None:intergenic |
| ACCGCCAAATCCACCGGATA+AGG | 0.446811 | 7.3:+32585146 | None:intergenic |
| GTGGTGGGTGCCGGCATCGG+TGG | 0.452113 | 7.3:+32585200 | None:intergenic |
| CGTGTCTTGGTTGATGCCTC+TGG | 0.452529 | 7.3:-32585020 | MS.gene42100:CDS |
| CGATCTTGTATTGTTTATGC+TGG | 0.461622 | 7.3:-32578088 | MS.gene42100:CDS |
| TTCACGACCGCCAAATCCAC+CGG | 0.471320 | 7.3:+32585140 | None:intergenic |
| TCCTTATCCGGTGGATTTGG+CGG | 0.474806 | 7.3:-32585147 | MS.gene42100:CDS |
| TTGCGACGTAGGGTGGTGAT+GGG | 0.485246 | 7.3:+32584993 | None:intergenic |
| TCCTTATCAACCACCGATGC+CGG | 0.493777 | 7.3:-32585210 | MS.gene42100:CDS |
| ATGAAAGCGTAATCAACATT+GGG | 0.498374 | 7.3:+32577857 | None:intergenic |
| TAAAGTCAAAGGTAGTTGGT+TGG | 0.499063 | 7.3:-32578113 | MS.gene42100:CDS |
| CCGAATATGGTGGTGGTGGT+GGG | 0.515761 | 7.3:+32585185 | None:intergenic |
| AAACTAGATGTGTTTCTAGC+TGG | 0.516765 | 7.3:-32578163 | MS.gene42100:CDS |
| TATGAAAGCGTAATCAACAT+TGG | 0.519673 | 7.3:+32577856 | None:intergenic |
| CAACCAAGACACGGCGGTCA+CGG | 0.526860 | 7.3:+32585030 | None:intergenic |
| AACGTTGTGTTTAAAGTGAG+AGG | 0.527433 | 7.3:-32585068 | MS.gene42100:CDS |
| GATAGACAGTGACTGCGAAA+TGG | 0.528270 | 7.3:+32577879 | None:intergenic |
| AACGGTGAAGTTGTCTGCCA+TGG | 0.529412 | 7.3:+32585106 | None:intergenic |
| TACTAACAACATTGTTGCAC+AGG | 0.530405 | 7.3:-32578062 | MS.gene42100:intron |
| TGTTGATTACGCTTTCATAG+TGG | 0.534696 | 7.3:-32577852 | MS.gene42100:CDS |
| TGTACATACCTTGCGACGTA+GGG | 0.572581 | 7.3:+32584983 | None:intergenic |
| AGTGACTATGCATGATCGGT+GGG | 0.580863 | 7.3:-32578266 | MS.gene42100:CDS |
| ATATTGTGCTCCTTATCCGG+TGG | 0.590508 | 7.3:-32585156 | MS.gene42100:CDS |
| CATCACCACCCTACGTCGCA+AGG | 0.590882 | 7.3:-32584991 | MS.gene42100:intron |
| TTCACCGTTAAAGATGTCAA+TGG | 0.593126 | 7.3:-32585092 | MS.gene42100:CDS |
| CCAAGACACGGCGGTCACGG+AGG | 0.598529 | 7.3:+32585033 | None:intergenic |
| CAAGACACGGCGGTCACGGA+GGG | 0.598937 | 7.3:+32585034 | None:intergenic |
| TAGTGACTATGCATGATCGG+TGG | 0.614019 | 7.3:-32578267 | MS.gene42100:CDS |
| GGTGGTGATGGGGTTACCAG+AGG | 0.631675 | 7.3:+32585004 | None:intergenic |
| AGAGCCGAATATGGTGGTGG+TGG | 0.638341 | 7.3:+32585181 | None:intergenic |
| ACATACCTTGCGACGTAGGG+TGG | 0.638701 | 7.3:+32584986 | None:intergenic |
| TTGTACATACCTTGCGACGT+AGG | 0.644546 | 7.3:+32584982 | None:intergenic |
| AGATAGTGACTATGCATGAT+CGG | 0.644746 | 7.3:-32578270 | MS.gene42100:CDS |
| TGCGACGTAGGGTGGTGATG+GGG | 0.652061 | 7.3:+32584994 | None:intergenic |
| CAGAGGCATCAACCAAGACA+CGG | 0.658669 | 7.3:+32585021 | None:intergenic |
| TTGAGAGCCGAATATGGTGG+TGG | 0.662726 | 7.3:+32585178 | None:intergenic |
| GATCGGTGGGAAGCTTACAG+AGG | 0.712766 | 7.3:-32578253 | MS.gene42100:CDS |
| ATATTGAGAGCCGAATATGG+TGG | 0.741009 | 7.3:+32585175 | None:intergenic |
| AGGCATCAACCAAGACACGG+CGG | 0.771771 | 7.3:+32585024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 32577808 | 32585240 | 32577808 | ID=MS.gene42100 |
| chr7.3 | mRNA | 32577808 | 32585240 | 32577808 | ID=MS.gene42100.t1;Parent=MS.gene42100 |
| chr7.3 | exon | 32584992 | 32585240 | 32584992 | ID=MS.gene42100.t1.exon1;Parent=MS.gene42100.t1 |
| chr7.3 | CDS | 32584992 | 32585240 | 32584992 | ID=cds.MS.gene42100.t1;Parent=MS.gene42100.t1 |
| chr7.3 | exon | 32578063 | 32578290 | 32578063 | ID=MS.gene42100.t1.exon2;Parent=MS.gene42100.t1 |
| chr7.3 | CDS | 32578063 | 32578290 | 32578063 | ID=cds.MS.gene42100.t1;Parent=MS.gene42100.t1 |
| chr7.3 | exon | 32577808 | 32577945 | 32577808 | ID=MS.gene42100.t1.exon3;Parent=MS.gene42100.t1 |
| chr7.3 | CDS | 32577808 | 32577945 | 32577808 | ID=cds.MS.gene42100.t1;Parent=MS.gene42100.t1 |
| Gene Sequence |
| Protein sequence |
