AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87038


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87038.t1 MTR_7g072120 81.905 210 30 4 1 202 1 210 1.51e-126 355
MS.gene87038.t1 MTR_7g072170 79.524 210 35 2 1 202 1 210 2.91e-123 348
MS.gene87038.t1 MTR_7g072270 78.744 207 38 1 1 201 1 207 5.09e-123 347
MS.gene87038.t1 MTR_7g072140 78.095 210 38 2 1 202 1 210 1.60e-121 343
MS.gene87038.t1 MTR_7g072250 66.820 217 51 3 1 200 25 237 3.12e-99 287
MS.gene87038.t1 MTR_7g072270 77.576 165 31 1 1 159 1 165 4.99e-95 274
MS.gene87038.t1 MTR_7g071710 95.763 118 5 0 86 203 1 118 4.41e-81 237
MS.gene87038.t1 MTR_4g029430 56.345 197 81 2 6 201 5 197 5.85e-79 234
MS.gene87038.t1 MTR_1g014180 51.613 186 80 5 16 196 5 185 4.99e-58 181
MS.gene87038.t1 MTR_1g014150 46.196 184 91 4 14 195 11 188 2.13e-46 151
MS.gene87038.t1 MTR_1g014160 40.426 188 87 6 26 195 59 239 2.42e-30 112
MS.gene87038.t1 MTR_2g103840 31.138 167 107 5 39 201 42 204 2.35e-22 90.9
MS.gene87038.t1 MTR_5g074550 30.556 180 110 6 26 195 17 191 1.79e-20 85.1
MS.gene87038.t1 MTR_4g010180 26.738 187 130 6 20 202 11 194 8.15e-20 83.6
MS.gene87038.t1 MTR_2g103850 29.762 168 110 5 39 202 43 206 5.16e-19 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87038.t1 AT5G01750 57.000 200 85 1 4 202 15 214 3.74e-91 266
MS.gene87038.t1 AT3G11740 58.421 190 77 2 15 202 2 191 5.28e-82 242
MS.gene87038.t1 AT2G14560 52.151 186 88 1 16 200 7 192 1.19e-70 214
MS.gene87038.t1 AT2G14560 50.510 196 95 2 6 200 58 252 1.16e-69 213
MS.gene87038.t1 AT1G33840 46.445 211 101 2 1 199 1 211 4.22e-67 205
MS.gene87038.t1 AT5G01750 55.063 158 70 1 4 160 15 172 2.17e-66 202
MS.gene87038.t1 AT2G14560 48.171 164 83 2 9 171 1 163 1.03e-54 172
MS.gene87038.t1 AT2G14560 49.045 157 78 2 6 161 58 213 1.14e-53 172
MS.gene87038.t1 AT1G33840 42.775 173 87 2 1 161 1 173 5.03e-49 158
MS.gene87038.t1 AT3G16900 42.162 185 87 4 16 199 15 180 7.07e-49 157
MS.gene87038.t1 AT3G56180 35.567 194 118 4 12 201 14 204 3.64e-36 126
MS.gene87038.t1 AT3G56180 35.052 194 117 5 12 201 14 202 7.28e-34 120
MS.gene87038.t1 AT3G10986 32.632 190 121 4 15 197 3 192 3.10e-29 107
MS.gene87038.t1 AT3G15810 30.952 168 106 6 39 202 44 205 1.57e-23 93.6
MS.gene87038.t1 AT1G80120 33.333 168 105 4 39 202 30 194 1.90e-19 82.8
MS.gene87038.t1 AT2G38640 31.953 169 106 5 33 198 30 192 4.06e-17 76.3
MS.gene87038.t1 AT5G20640 28.177 181 113 5 36 201 33 211 4.62e-15 71.2
MS.gene87038.t1 AT2G05910 29.586 169 98 8 39 198 28 184 1.19e-13 67.0
MS.gene87038.t1 AT5G41590 25.000 176 122 4 33 198 44 219 1.39e-11 61.6

Find 51 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACAAACATCTTCTT+TGG 0.257604 7.4:+32420109 None:intergenic
GGATTTAGCGGTCGTGAAAA+AGG 0.322249 7.4:-32421024 MS.gene87038:CDS
GCACCCACCACCACCATATT+CGG 0.351246 7.4:-32421074 MS.gene87038:CDS
ACAATATTGAGAGCCGAATA+TGG 0.363340 7.4:+32421061 None:intergenic
TAGGGTGGTGATGGGGTTAC+CGG 0.377318 7.4:+32420890 None:intergenic
GTTGCCATTGACATCTTTAA+CGG 0.392620 7.4:+32420977 None:intergenic
AATATGGTGGTGGTGGGTGC+TGG 0.394560 7.4:+32421077 None:intergenic
ACCGCTAAATCCACCGGATA+AGG 0.395830 7.4:+32421035 None:intergenic
AGCTAGAAACACATCTAGTT+TGG 0.401309 7.4:+32420139 None:intergenic
TCAATATTGTGCTCCTTATC+CGG 0.417584 7.4:-32421048 MS.gene87038:CDS
CCTCCGTGACCGCCGTGTCT+TGG 0.425443 7.4:-32420922 MS.gene87038:CDS
GTGGTGGTGGGTGCTGGCAT+CGG 0.428409 7.4:+32421083 None:intergenic
CTTGCGACGTAGGGTGGTGA+TGG 0.443247 7.4:+32420881 None:intergenic
GCTGGCATCGGTGGTTGATA+AGG 0.448493 7.4:+32421095 None:intergenic
TCCTTATCCGGTGGATTTAG+CGG 0.450209 7.4:-32421036 MS.gene87038:CDS
TTGCGACGTAGGGTGGTGAT+GGG 0.485246 7.4:+32420882 None:intergenic
CGTGTCTTGGTTGATGCCTC+CGG 0.494853 7.4:-32420909 MS.gene87038:CDS
GTGGTGGGTGCTGGCATCGG+TGG 0.497728 7.4:+32421086 None:intergenic
ATGAAAGCGTAATCAACATT+GGG 0.498374 7.4:+32419831 None:intergenic
TAAAGTCAAAGGTAGTTGGT+TGG 0.499063 7.4:-32420087 MS.gene87038:CDS
AACAATGTTGTTAGTATCAC+CGG 0.501905 7.4:+32420043 None:intergenic
TATGAAAGCGTAATCAACAT+TGG 0.519673 7.4:+32419830 None:intergenic
AAACTAGATGTGTTTCTAGC+TGG 0.525823 7.4:-32420137 MS.gene87038:CDS
CAACCAAGACACGGCGGTCA+CGG 0.526860 7.4:+32420919 None:intergenic
GATAGACAGTGACTGCGAAA+TGG 0.528270 7.4:+32419853 None:intergenic
AACGGTGAAGTTGTCTGCCA+TGG 0.529412 7.4:+32420995 None:intergenic
TACTAACAACATTGTTGCAC+AGG 0.530405 7.4:-32420036 MS.gene87038:intron
TGTTGATTACGCTTTCATAG+TGG 0.534696 7.4:-32419826 MS.gene87038:CDS
TTCACGACCGCTAAATCCAC+CGG 0.537903 7.4:+32421029 None:intergenic
CGATCTTGTATTGTTCATGC+CGG 0.561038 7.4:-32420062 MS.gene87038:CDS
AACGTCGTGTTTAAAGTGAA+AGG 0.567690 7.4:-32420957 MS.gene87038:CDS
AGAGCCGAATATGGTGGTGG+TGG 0.571753 7.4:+32421070 None:intergenic
TGTACATACCTTGCGACGTA+GGG 0.572581 7.4:+32420872 None:intergenic
AGTGACTATGCATGATCGGT+GGG 0.580863 7.4:-32420240 MS.gene87038:CDS
ATATTGTGCTCCTTATCCGG+TGG 0.590508 7.4:-32421045 MS.gene87038:CDS
CATCACCACCCTACGTCGCA+AGG 0.590882 7.4:-32420880 MS.gene87038:intron
TTCACCGTTAAAGATGTCAA+TGG 0.593126 7.4:-32420981 MS.gene87038:CDS
CCAAGACACGGCGGTCACGG+AGG 0.598529 7.4:+32420922 None:intergenic
CAAGACACGGCGGTCACGGA+GGG 0.598937 7.4:+32420923 None:intergenic
TAGTGACTATGCATGATCGG+TGG 0.614019 7.4:-32420241 MS.gene87038:CDS
GGTGGTGATGGGGTTACCGG+AGG 0.620164 7.4:+32420893 None:intergenic
ACATACCTTGCGACGTAGGG+TGG 0.638701 7.4:+32420875 None:intergenic
TTGTACATACCTTGCGACGT+AGG 0.644546 7.4:+32420871 None:intergenic
AGATAGTGACTATGCATGAT+CGG 0.644746 7.4:-32420244 MS.gene87038:CDS
TGCGACGTAGGGTGGTGATG+GGG 0.652061 7.4:+32420883 None:intergenic
CGGAGGCATCAACCAAGACA+CGG 0.654823 7.4:+32420910 None:intergenic
TTGAGAGCCGAATATGGTGG+TGG 0.662726 7.4:+32421067 None:intergenic
GAGCCGAATATGGTGGTGGT+GGG 0.669874 7.4:+32421071 None:intergenic
GATCGGTGGGAAGCTTACAG+AGG 0.712766 7.4:-32420227 MS.gene87038:CDS
ATATTGAGAGCCGAATATGG+TGG 0.741009 7.4:+32421064 None:intergenic
AGGCATCAACCAAGACACGG+CGG 0.796971 7.4:+32420913 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCAACTAAAAAAAATA+TGG - chr7.4:32420435-32420454 MS.gene87038:intron 10.0%
!!! TTATTTTTTAAATAAAGAGT+AGG - chr7.4:32420126-32420145 MS.gene87038:CDS 10.0%
!! AGTATGTGAATTTCAATAAT+TGG - chr7.4:32420477-32420496 MS.gene87038:intron 20.0%
!! TTGAGATATATGAAGTATAA+TGG + chr7.4:32420580-32420599 None:intergenic 20.0%
!!! ATATAAGGTTTTACTTTGAT+TGG - chr7.4:32420904-32420923 MS.gene87038:CDS 20.0%
!!! TGCTCAATATTTTAATTTTG+TGG - chr7.4:32420076-32420095 MS.gene87038:CDS 20.0%
!!! TGTAAATTCAAGAGTATATA+AGG - chr7.4:32420889-32420908 MS.gene87038:CDS 20.0%
! AAAATCACAAACATCTTCTT+TGG + chr7.4:32420780-32420799 None:intergenic 25.0%
! TTTGATTGGATTATCACTTA+AGG - chr7.4:32420918-32420937 MS.gene87038:CDS 25.0%
!! GATTCATTAAGTTGTACTAT+TGG - chr7.4:32420616-32420635 MS.gene87038:intron 25.0%
!!! AAAAATATGGTTTTGCTCAA+TGG - chr7.4:32420448-32420467 MS.gene87038:intron 25.0%
!!! ATAACTTTTTTTTTTCGTGC+AGG + chr7.4:32420413-32420432 None:intergenic 25.0%
!!! ATTTTAAAGTCAAAGGTAGT+TGG - chr7.4:32420795-32420814 MS.gene87038:intron 25.0%
!!! GTTTGTGATTTTAAAGTCAA+AGG - chr7.4:32420788-32420807 MS.gene87038:intron 25.0%
AACAATGTTGTTAGTATCAC+CGG + chr7.4:32420846-32420865 None:intergenic 30.0%
! ATGAAAGCGTAATCAACATT+GGG + chr7.4:32421058-32421077 None:intergenic 30.0%
! TATGAAAGCGTAATCAACAT+TGG + chr7.4:32421059-32421078 None:intergenic 30.0%
ACAATATTGAGAGCCGAATA+TGG + chr7.4:32419828-32419847 None:intergenic 35.0%
AGATAGTGACTATGCATGAT+CGG - chr7.4:32420642-32420661 MS.gene87038:intron 35.0%
AGCTAGAAACACATCTAGTT+TGG + chr7.4:32420750-32420769 None:intergenic 35.0%
GTTGCCATTGACATCTTTAA+CGG + chr7.4:32419912-32419931 None:intergenic 35.0%
TAAAGTCAAAGGTAGTTGGT+TGG - chr7.4:32420799-32420818 MS.gene87038:intron 35.0%
TACTAACAACATTGTTGCAC+AGG - chr7.4:32420850-32420869 MS.gene87038:intron 35.0%
TCAATATTGTGCTCCTTATC+CGG - chr7.4:32419838-32419857 MS.gene87038:CDS 35.0%
TTCACCGTTAAAGATGTCAA+TGG - chr7.4:32419905-32419924 MS.gene87038:intron 35.0%
! AACGTCGTGTTTAAAGTGAA+AGG - chr7.4:32419929-32419948 MS.gene87038:intron 35.0%
! TGTTGATTACGCTTTCATAG+TGG - chr7.4:32421060-32421079 MS.gene87038:CDS 35.0%
!! AAACTAGATGTGTTTCTAGC+TGG - chr7.4:32420749-32420768 MS.gene87038:intron 35.0%
ATATTGAGAGCCGAATATGG+TGG + chr7.4:32419825-32419844 None:intergenic 40.0%
CGATCTTGTATTGTTCATGC+CGG - chr7.4:32420824-32420843 MS.gene87038:intron 40.0%
CGTGAAAAAGGTAATGACCA+TGG - chr7.4:32419874-32419893 MS.gene87038:CDS 40.0%
AGTGACTATGCATGATCGGT+GGG - chr7.4:32420646-32420665 MS.gene87038:intron 45.0%
ATATTGTGCTCCTTATCCGG+TGG - chr7.4:32419841-32419860 MS.gene87038:CDS 45.0%
GATAGACAGTGACTGCGAAA+TGG + chr7.4:32421036-32421055 None:intergenic 45.0%
GGATTTAGCGGTCGTGAAAA+AGG - chr7.4:32419862-32419881 MS.gene87038:CDS 45.0%
TAGTGACTATGCATGATCGG+TGG - chr7.4:32420645-32420664 MS.gene87038:intron 45.0%
TGTACATACCTTGCGACGTA+GGG + chr7.4:32420017-32420036 None:intergenic 45.0%
TTGTACATACCTTGCGACGT+AGG + chr7.4:32420018-32420037 None:intergenic 45.0%
! TCCTTATCCGGTGGATTTAG+CGG - chr7.4:32419850-32419869 MS.gene87038:CDS 45.0%
!!! TTTTTACTTTTTTTTTTTTT+TGG + chr7.4:32420514-32420533 None:intergenic 5.0%
AACGGTGAAGTTGTCTGCCA+TGG + chr7.4:32419894-32419913 None:intergenic 50.0%
ACCGCTAAATCCACCGGATA+AGG + chr7.4:32419854-32419873 None:intergenic 50.0%
CAACCACGTGAGTCTACTCT+CGG + chr7.4:32420156-32420175 None:intergenic 50.0%
TTCACGACCGCTAAATCCAC+CGG + chr7.4:32419860-32419879 None:intergenic 50.0%
TTGAGAGCCGAATATGGTGG+TGG + chr7.4:32419822-32419841 None:intergenic 50.0%
ACATACCTTGCGACGTAGGG+TGG + chr7.4:32420014-32420033 None:intergenic 55.0%
AGGCATCAACCAAGACACGG+CGG + chr7.4:32419976-32419995 None:intergenic 55.0%
CATCCGAGAGTAGACTCACG+TGG - chr7.4:32420150-32420169 MS.gene87038:CDS 55.0%
GATCGGTGGGAAGCTTACAG+AGG - chr7.4:32420659-32420678 MS.gene87038:intron 55.0%
GCACCCACCACCACCATATT+CGG - chr7.4:32419812-32419831 MS.gene87038:CDS 55.0%
TTGCGACGTAGGGTGGTGAT+GGG + chr7.4:32420007-32420026 None:intergenic 55.0%
! CGTGTCTTGGTTGATGCCTC+CGG - chr7.4:32419977-32419996 MS.gene87038:intron 55.0%
!! AATATGGTGGTGGTGGGTGC+TGG + chr7.4:32419812-32419831 None:intergenic 55.0%
!! AGAGCCGAATATGGTGGTGG+TGG + chr7.4:32419819-32419838 None:intergenic 55.0%
!! CGGAGGCATCAACCAAGACA+CGG + chr7.4:32419979-32419998 None:intergenic 55.0%
!! GAGCCGAATATGGTGGTGGT+GGG + chr7.4:32419818-32419837 None:intergenic 55.0%
!! GCTGGCATCGGTGGTTGATA+AGG + chr7.4:32419794-32419813 None:intergenic 55.0%
!! TAGGGTGGTGATGGGGTTAC+CGG + chr7.4:32419999-32420018 None:intergenic 55.0%
CAACCAAGACACGGCGGTCA+CGG + chr7.4:32419970-32419989 None:intergenic 60.0%
CATCACCACCCTACGTCGCA+AGG - chr7.4:32420006-32420025 MS.gene87038:intron 60.0%
CTTGCGACGTAGGGTGGTGA+TGG + chr7.4:32420008-32420027 None:intergenic 60.0%
! TGCGACGTAGGGTGGTGATG+GGG + chr7.4:32420006-32420025 None:intergenic 60.0%
CAAGACACGGCGGTCACGGA+GGG + chr7.4:32419966-32419985 None:intergenic 65.0%
!! GGTGGTGATGGGGTTACCGG+AGG + chr7.4:32419996-32420015 None:intergenic 65.0%
!! GTGGTGGTGGGTGCTGGCAT+CGG + chr7.4:32419806-32419825 None:intergenic 65.0%
CCAAGACACGGCGGTCACGG+AGG + chr7.4:32419967-32419986 None:intergenic 70.0%
CCTCCGTGACCGCCGTGTCT+TGG - chr7.4:32419964-32419983 MS.gene87038:intron 70.0%
!! GTGGTGGGTGCTGGCATCGG+TGG + chr7.4:32419803-32419822 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 32419782 32421126 32419782 ID=MS.gene87038
chr7.4 mRNA 32419782 32421126 32419782 ID=MS.gene87038.t1;Parent=MS.gene87038
chr7.4 exon 32420881 32421126 32420881 ID=MS.gene87038.t1.exon1;Parent=MS.gene87038.t1
chr7.4 CDS 32420881 32421126 32420881 ID=cds.MS.gene87038.t1;Parent=MS.gene87038.t1
chr7.4 exon 32420037 32420264 32420037 ID=MS.gene87038.t1.exon2;Parent=MS.gene87038.t1
chr7.4 CDS 32420037 32420264 32420037 ID=cds.MS.gene87038.t1;Parent=MS.gene87038.t1
chr7.4 exon 32419782 32419919 32419782 ID=MS.gene87038.t1.exon3;Parent=MS.gene87038.t1
chr7.4 CDS 32419782 32419919 32419782 ID=cds.MS.gene87038.t1;Parent=MS.gene87038.t1
Gene Sequence

>MS.gene87038

ATGGTTTATCCTTATCAACCACCGATGCCAGCACCCACCACCACCATATTCGGCTCTCAATATTGTGCTCCTTATCCGGTGGATTTAGCGGTCGTGAAAAAGGTAATGACCATGGCAGACAACTTCACCGTTAAAGATGTCAATGGCAACGTCGTGTTTAAAGTGAAAGGTTCTCTCTTAACCCTCCGTGACCGCCGTGTCTTGGTTGATGCCTCCGGTAACCCCATCACCACCCTACGTCGCAAGGTATGTACAACTTTATTAAGAAACTTTGCTTTCATATTTTTATTTTAATGCTCAATATTTTAATTTTGTGGTGATGAGTTTTCTACATATATGATATTTTATTTTTTAAATAAAGAGTAGGACATCCGAGAGTAGACTCACGTGGTTGCACGTTGAACTTTATTTTTAGATAAGACGTAACTTTATTACATTTAAATAATATTTAATACGAAAAATGTATGTTGTAAAATTATATATTTTGTAACTTGAAACTCTTTATATTAAAGTTTGTAAGTTAAAAATTATGCAATTATAATTTAGTTGATGATTAATTTTAATAATCTTTTCAAAGAGAAATATGATCTATTTTACTCATACGAGCTCATATATAAGTTATTCACTTCCTGCACGAAAAAAAAAAGTTATTCATTATCAACTAAAAAAAATATGGTTTTGCTCAATGGAGTCTCAGTATGTGAATTTCAATAATTGGATGATTGAAATCCAAAAAAAAAAAAAAGTAAAAACATATATACTAGAGTGCTATTTATTGTTAAAAATTCATATTATCCATTATACTTCATATATCTCAATAAAGTGTTGTTTAATGATTCATTAAGTTGTACTATTGGTGCAGATAGTGACTATGCATGATCGGTGGGAAGCTTACAGAGGAGAAAGCACAAATTCTAAAGATCTTATATTTACATTGAGAAAATCATCGCTATTGCAGTTGAAGACCAAACTAGATGTGTTTCTAGCTGGTAATACCAAAGAAGATGTTTGTGATTTTAAAGTCAAAGGTAGTTGGTTGGAACGATCTTGTATTGTTCATGCCGGTGATACTAACAACATTGTTGCACAGGTAATTAATAAGCATCTTGTAAATTCAAGAGTATATAAGGTTTTACTTTGATTGGATTATCACTTAAGGACATACGCTAATCATAAATCAATTTTTGTGATATTTTTGTTACTACAGATGCACAAAAAGCACACTATTCAGAGTATTTTAATTGATAAAGACCATTTCGCAGTCACTGTCTATCCCAATGTTGATTACGCTTTCATAGTGGCATTAATCGCGATTCTTAACGAGATTAACGAAGATGATGATTGA

Protein sequence

>MS.gene87038.t1

MVYPYQPPMPAPTTTIFGSQYCAPYPVDLAVVKKVMTMADNFTVKDVNGNVVFKVKGSLLTLRDRRVLVDASGNPITTLRRKIVTMHDRWEAYRGESTNSKDLIFTLRKSSLLQLKTKLDVFLAGNTKEDVCDFKVKGSWLERSCIVHAGDTNNIVAQMHKKHTIQSILIDKDHFAVTVYPNVDYAFIVALIAILNEINEDDD