AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42097


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42097.t1 MTR_7g072120 84.021 194 30 1 2 194 17 210 3.09e-125 352
MS.gene42097.t1 MTR_7g072270 80.829 193 37 0 1 193 15 207 1.55e-119 337
MS.gene42097.t1 MTR_7g072170 81.538 195 35 1 1 194 16 210 1.80e-119 338
MS.gene42097.t1 MTR_7g072140 80.000 195 38 1 1 194 16 210 2.17e-117 332
MS.gene42097.t1 MTR_7g072250 68.812 202 49 2 1 192 40 237 1.32e-97 283
MS.gene42097.t1 MTR_7g072270 80.132 151 30 0 1 151 15 165 1.39e-91 265
MS.gene42097.t1 MTR_7g071710 94.915 118 6 0 78 195 1 118 2.03e-81 237
MS.gene42097.t1 MTR_4g029430 59.259 189 72 2 6 193 13 197 2.83e-80 238
MS.gene42097.t1 MTR_1g014180 50.538 186 82 5 8 188 5 185 9.05e-58 180
MS.gene42097.t1 MTR_1g014150 45.652 184 92 4 6 187 11 188 4.21e-46 150
MS.gene42097.t1 MTR_1g014160 39.362 188 89 6 18 187 59 239 5.54e-29 108
MS.gene42097.t1 MTR_2g103840 30.539 167 108 5 31 193 42 204 4.74e-23 92.4
MS.gene42097.t1 MTR_5g074550 30.556 180 110 6 18 187 17 191 8.83e-21 85.5
MS.gene42097.t1 MTR_4g010180 27.545 167 115 5 31 194 31 194 9.19e-21 85.9
MS.gene42097.t1 MTR_2g103850 29.167 168 111 5 31 194 43 206 6.55e-20 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42097.t1 AT5G01750 57.868 197 78 2 3 194 18 214 6.13e-90 263
MS.gene42097.t1 AT3G11740 58.730 189 76 2 8 194 3 191 1.01e-82 243
MS.gene42097.t1 AT2G14560 50.777 193 93 2 1 192 1 192 1.51e-70 213
MS.gene42097.t1 AT2G14560 50.777 193 93 2 1 192 61 252 2.20e-69 212
MS.gene42097.t1 AT1G33840 50.811 185 90 1 8 191 27 211 1.98e-68 208
MS.gene42097.t1 AT5G01750 56.129 155 63 2 3 152 18 172 2.28e-65 199
MS.gene42097.t1 AT2G14560 49.351 154 76 2 1 153 1 153 9.09e-55 172
MS.gene42097.t1 AT2G14560 49.351 154 76 2 1 153 61 213 1.17e-53 171
MS.gene42097.t1 AT1G33840 47.619 147 76 1 8 153 27 173 2.20e-50 161
MS.gene42097.t1 AT3G16900 42.703 185 86 4 8 191 15 180 4.08e-49 158
MS.gene42097.t1 AT3G56180 35.567 194 118 4 4 193 14 204 6.49e-37 127
MS.gene42097.t1 AT3G56180 35.052 194 117 5 4 193 14 202 1.22e-34 121
MS.gene42097.t1 AT3G10986 33.684 190 119 4 7 189 3 192 1.39e-30 111
MS.gene42097.t1 AT3G15810 32.143 168 104 6 31 194 44 205 2.71e-24 95.5
MS.gene42097.t1 AT1G80120 32.738 168 106 4 31 194 30 194 1.38e-19 82.8
MS.gene42097.t1 AT2G38640 31.953 169 106 5 25 190 30 192 1.57e-16 74.3
MS.gene42097.t1 AT5G20640 28.729 181 112 5 28 193 33 211 7.23e-15 70.5
MS.gene42097.t1 AT2G05910 28.402 169 100 7 31 190 28 184 1.75e-13 66.2
MS.gene42097.t1 AT5G41590 24.432 176 123 4 25 190 44 219 3.77e-11 60.5

Find 51 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGTCACAAATATCTTCTT+TGG 0.245817 7.3:+32513513 None:intergenic
CTTGCGACGTAGGGTGGTTA+TGG 0.262386 7.3:+32514252 None:intergenic
ATTTGGTCTTCAACTGCAAT+AGG 0.271974 7.3:+32513560 None:intergenic
TGATCGGTGGGAAGCTTATA+GGG 0.301757 7.3:-32513632 MS.gene42097:CDS
ATGATCGGTGGGAAGCTTAT+AGG 0.312103 7.3:-32513633 MS.gene42097:CDS
GGATTTGGCGGTCGTGAAAA+AGG 0.350662 7.3:-32514395 MS.gene42097:CDS
TTGCGACGTAGGGTGGTTAT+GGG 0.360381 7.3:+32514253 None:intergenic
GCACCCACCACCACCATATT+CGG 0.364115 7.3:-32514445 MS.gene42097:CDS
AGCTAGAAACACATCTAATT+TGG 0.366171 7.3:+32513543 None:intergenic
GTGGTGGTGGGTGCCGGCAT+CGG 0.380802 7.3:+32514454 None:intergenic
ACAATATTGAGATCCGAATA+TGG 0.391065 7.3:+32514432 None:intergenic
GTTGCCATTGACATCTTTAA+CGG 0.392620 7.3:+32514348 None:intergenic
TGCTCCGTATCCAGTGGATT+TGG 0.401326 7.3:-32514410 MS.gene42097:CDS
TTTGGTCTTCAACTGCAATA+GGG 0.412568 7.3:+32513561 None:intergenic
ACTTTAAAGTCAAAGGTAGT+TGG 0.429380 7.3:-32513495 MS.gene42097:CDS
CGATCTTGCATTATTTATGC+AGG 0.445806 7.3:-32513466 MS.gene42097:CDS
AATATGGTGGTGGTGGGTGC+CGG 0.451025 7.3:+32514448 None:intergenic
CCTCCGTGATCGCCGTGTCT+TGG 0.452683 7.3:-32514293 MS.gene42097:CDS
TTCACGACCGCCAAATCCAC+TGG 0.473461 7.3:+32514400 None:intergenic
CGTGTCTTGGTTGATGCTGC+TGG 0.478066 7.3:-32514280 MS.gene42097:CDS
ATGAAAGCGTAATCAACATT+GGG 0.498374 7.3:+32513196 None:intergenic
AAATTAGATGTGTTTCTAGC+TGG 0.501483 7.3:-32513541 MS.gene42097:CDS
GCGATCACGGAGGGTTAAGA+AGG 0.515037 7.3:+32514303 None:intergenic
TAAAGTCAAAGGTAGTTGGT+TGG 0.515691 7.3:-32513491 MS.gene42097:CDS
TATGAAAGCGTAATCAACAT+TGG 0.519673 7.3:+32513195 None:intergenic
ATTTGTGACTTTAAAGTCAA+AGG 0.520188 7.3:-32513502 MS.gene42097:CDS
AACGGTGAAGTTGTCTGCCA+TGG 0.529412 7.3:+32514366 None:intergenic
TACTAACAACATTGTTGCAC+AGG 0.530405 7.3:-32513440 MS.gene42097:intron
CAACCAAGACACGGCGATCA+CGG 0.533888 7.3:+32514290 None:intergenic
TGTTGATTACGCTTTCATAG+TGG 0.534696 7.3:-32513191 MS.gene42097:CDS
TGAAAGTACCTTGCGACGTA+GGG 0.537454 7.3:+32514243 None:intergenic
ACCGCCAAATCCACTGGATA+CGG 0.545681 7.3:+32514406 None:intergenic
AGATCCGAATATGGTGGTGG+TGG 0.561587 7.3:+32514441 None:intergenic
TCCGTATCCAGTGGATTTGG+CGG 0.571007 7.3:-32514407 MS.gene42097:CDS
TTGAAAGTACCTTGCGACGT+AGG 0.573670 7.3:+32514242 None:intergenic
AGTGACTATGCATGATCGGT+GGG 0.580863 7.3:-32513644 MS.gene42097:CDS
TTCACCGTTAAAGATGTCAA+TGG 0.593126 7.3:-32514352 MS.gene42097:CDS
CATAACCACCCTACGTCGCA+AGG 0.600007 7.3:-32514251 MS.gene42097:intron
CAAGACACGGCGATCACGGA+GGG 0.603514 7.3:+32514294 None:intergenic
AAGTACCTTGCGACGTAGGG+TGG 0.605892 7.3:+32514246 None:intergenic
TAGTGACTATGCATGATCGG+TGG 0.614019 7.3:-32513645 MS.gene42097:CDS
AACGTCGTGTTCAAAGTGAA+AGG 0.614534 7.3:-32514328 MS.gene42097:CDS
GATCGGTGGGAAGCTTATAG+GGG 0.623792 7.3:-32513631 MS.gene42097:CDS
AGATAGTGACTATGCATGAT+CGG 0.644746 7.3:-32513648 MS.gene42097:CDS
ATATTGTGCTCCGTATCCAG+TGG 0.650396 7.3:-32514416 MS.gene42097:CDS
TTGAGATCCGAATATGGTGG+TGG 0.650739 7.3:+32514438 None:intergenic
TGCGACGTAGGGTGGTTATG+GGG 0.653394 7.3:+32514254 None:intergenic
CAGCAGCATCAACCAAGACA+CGG 0.658669 7.3:+32514281 None:intergenic
GATCCGAATATGGTGGTGGT+GGG 0.674694 7.3:+32514442 None:intergenic
CCAAGACACGGCGATCACGG+AGG 0.703726 7.3:+32514293 None:intergenic
ATATTGAGATCCGAATATGG+TGG 0.748524 7.3:+32514435 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAAAAATAACTAGT+AGG - chr7.3:32513475-32513494 MS.gene42097:CDS 10.0%
!! TTTGTACCAAAAAAAATATA+AGG - chr7.3:32514236-32514255 MS.gene42097:intron 15.0%
!!! ATTCTATTAAGTTGTATTAT+TGG - chr7.3:32513924-32513943 MS.gene42097:intron 15.0%
!!! GTAAAACCTTATATTTTTTT+TGG + chr7.3:32514245-32514264 None:intergenic 15.0%
!! ATCATTAAACAACACTTTAT+TGG + chr7.3:32513906-32513925 None:intergenic 20.0%
!! TCATTAAACAACACTTTATT+GGG + chr7.3:32513905-32513924 None:intergenic 20.0%
!! TTTGATTGGATTATAACTTA+AGG - chr7.3:32514265-32514284 MS.gene42097:CDS 20.0%
!!! AGTATATACAAGAGTATATA+AGG - chr7.3:32514200-32514219 MS.gene42097:intron 20.0%
!!! ATATAAGGTTTTACTTTGAT+TGG - chr7.3:32514251-32514270 MS.gene42097:intron 20.0%
!!! TATTTTTTTTGGTACAAAAG+AGG + chr7.3:32514234-32514253 None:intergenic 20.0%
! AAAGTCACAAATATCTTCTT+TGG + chr7.3:32514088-32514107 None:intergenic 25.0%
! AATGTGTGAATTTCAACAAT+TGG - chr7.3:32513792-32513811 MS.gene42097:intron 25.0%
! TTGGGATATATGAAGTATAA+TGG + chr7.3:32513887-32513906 None:intergenic 25.0%
!!! ATTTGTGACTTTAAAGTCAA+AGG - chr7.3:32514096-32514115 MS.gene42097:intron 25.0%
!!! CAAAAAATTGTTTTGCTCAA+TGG - chr7.3:32513763-32513782 MS.gene42097:intron 25.0%
ACAATATTGAGATCCGAATA+TGG + chr7.3:32513169-32513188 None:intergenic 30.0%
AGCTAGAAACACATCTAATT+TGG + chr7.3:32514058-32514077 None:intergenic 30.0%
! ATGAAAGCGTAATCAACATT+GGG + chr7.3:32514405-32514424 None:intergenic 30.0%
! TATGAAAGCGTAATCAACAT+TGG + chr7.3:32514406-32514425 None:intergenic 30.0%
!! AAATTAGATGTGTTTCTAGC+TGG - chr7.3:32514057-32514076 MS.gene42097:intron 30.0%
!! ACTTTAAAGTCAAAGGTAGT+TGG - chr7.3:32514103-32514122 MS.gene42097:intron 30.0%
AGATAGTGACTATGCATGAT+CGG - chr7.3:32513950-32513969 MS.gene42097:intron 35.0%
ATATTGAGATCCGAATATGG+TGG + chr7.3:32513166-32513185 None:intergenic 35.0%
CGATCTTGCATTATTTATGC+AGG - chr7.3:32514132-32514151 MS.gene42097:intron 35.0%
GTTGCCATTGACATCTTTAA+CGG + chr7.3:32513253-32513272 None:intergenic 35.0%
TAAAGTCAAAGGTAGTTGGT+TGG - chr7.3:32514107-32514126 MS.gene42097:intron 35.0%
TACTAACAACATTGTTGCAC+AGG - chr7.3:32514158-32514177 MS.gene42097:intron 35.0%
TTCACCGTTAAAGATGTCAA+TGG - chr7.3:32513246-32513265 MS.gene42097:CDS 35.0%
! ATTTGGTCTTCAACTGCAAT+AGG + chr7.3:32514041-32514060 None:intergenic 35.0%
! TGTTGATTACGCTTTCATAG+TGG - chr7.3:32514407-32514426 MS.gene42097:CDS 35.0%
! TTTGGTCTTCAACTGCAATA+GGG + chr7.3:32514040-32514059 None:intergenic 35.0%
AACGTCGTGTTCAAAGTGAA+AGG - chr7.3:32513270-32513289 MS.gene42097:intron 40.0%
CGTGAAAAAGGTAATGACCA+TGG - chr7.3:32513215-32513234 MS.gene42097:CDS 40.0%
ACTAGTAGGACATCCGAGTA+TGG - chr7.3:32513489-32513508 MS.gene42097:CDS 45.0%
AGTGACTATGCATGATCGGT+GGG - chr7.3:32513954-32513973 MS.gene42097:intron 45.0%
ATATTGTGCTCCGTATCCAG+TGG - chr7.3:32513182-32513201 MS.gene42097:CDS 45.0%
ATGATCGGTGGGAAGCTTAT+AGG - chr7.3:32513965-32513984 MS.gene42097:intron 45.0%
CAACTACGTGAGTCCATACT+CGG + chr7.3:32513505-32513524 None:intergenic 45.0%
TAGTGACTATGCATGATCGG+TGG - chr7.3:32513953-32513972 MS.gene42097:intron 45.0%
TGATCGGTGGGAAGCTTATA+GGG - chr7.3:32513966-32513985 MS.gene42097:intron 45.0%
TTGAGATCCGAATATGGTGG+TGG + chr7.3:32513163-32513182 None:intergenic 45.0%
!! TGAAAGTACCTTGCGACGTA+GGG + chr7.3:32513358-32513377 None:intergenic 45.0%
!! TTGAAAGTACCTTGCGACGT+AGG + chr7.3:32513359-32513378 None:intergenic 45.0%
AACGGTGAAGTTGTCTGCCA+TGG + chr7.3:32513235-32513254 None:intergenic 50.0%
ACCGCCAAATCCACTGGATA+CGG + chr7.3:32513195-32513214 None:intergenic 50.0%
CAGCAGCATCAACCAAGACA+CGG + chr7.3:32513320-32513339 None:intergenic 50.0%
GATCGGTGGGAAGCTTATAG+GGG - chr7.3:32513967-32513986 MS.gene42097:intron 50.0%
GGATTTGGCGGTCGTGAAAA+AGG - chr7.3:32513203-32513222 MS.gene42097:CDS 50.0%
TTGCGACGTAGGGTGGTTAT+GGG + chr7.3:32513348-32513367 None:intergenic 50.0%
! TCCGTATCCAGTGGATTTGG+CGG - chr7.3:32513191-32513210 MS.gene42097:CDS 50.0%
! TGCTCCGTATCCAGTGGATT+TGG - chr7.3:32513188-32513207 MS.gene42097:CDS 50.0%
!! AGATCCGAATATGGTGGTGG+TGG + chr7.3:32513160-32513179 None:intergenic 50.0%
!! GATCCGAATATGGTGGTGGT+GGG + chr7.3:32513159-32513178 None:intergenic 50.0%
AAGTACCTTGCGACGTAGGG+TGG + chr7.3:32513355-32513374 None:intergenic 55.0%
CAACCAAGACACGGCGATCA+CGG + chr7.3:32513311-32513330 None:intergenic 55.0%
CATAACCACCCTACGTCGCA+AGG - chr7.3:32513347-32513366 MS.gene42097:intron 55.0%
CTTGCGACGTAGGGTGGTTA+TGG + chr7.3:32513349-32513368 None:intergenic 55.0%
GCACCCACCACCACCATATT+CGG - chr7.3:32513153-32513172 MS.gene42097:CDS 55.0%
GCGATCACGGAGGGTTAAGA+AGG + chr7.3:32513298-32513317 None:intergenic 55.0%
TGCGACGTAGGGTGGTTATG+GGG + chr7.3:32513347-32513366 None:intergenic 55.0%
TTCACGACCGCCAAATCCAC+TGG + chr7.3:32513201-32513220 None:intergenic 55.0%
! CGTGTCTTGGTTGATGCTGC+TGG - chr7.3:32513318-32513337 MS.gene42097:intron 55.0%
!! AATATGGTGGTGGTGGGTGC+CGG + chr7.3:32513153-32513172 None:intergenic 55.0%
CAAGACACGGCGATCACGGA+GGG + chr7.3:32513307-32513326 None:intergenic 60.0%
CCAAGACACGGCGATCACGG+AGG + chr7.3:32513308-32513327 None:intergenic 65.0%
CCTCCGTGATCGCCGTGTCT+TGG - chr7.3:32513305-32513324 MS.gene42097:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 32513147 32514473 32513147 ID=MS.gene42097
chr7.3 mRNA 32513147 32514473 32513147 ID=MS.gene42097.t1;Parent=MS.gene42097
chr7.3 exon 32514252 32514473 32514252 ID=MS.gene42097.t1.exon1;Parent=MS.gene42097.t1
chr7.3 CDS 32514252 32514473 32514252 ID=cds.MS.gene42097.t1;Parent=MS.gene42097.t1
chr7.3 exon 32513441 32513668 32513441 ID=MS.gene42097.t1.exon2;Parent=MS.gene42097.t1
chr7.3 CDS 32513441 32513668 32513441 ID=cds.MS.gene42097.t1;Parent=MS.gene42097.t1
chr7.3 exon 32513147 32513284 32513147 ID=MS.gene42097.t1.exon3;Parent=MS.gene42097.t1
chr7.3 CDS 32513147 32513284 32513147 ID=cds.MS.gene42097.t1;Parent=MS.gene42097.t1
Gene Sequence

>MS.gene42097

ATGCCGGCACCCACCACCACCATATTCGGATCTCAATATTGTGCTCCGTATCCAGTGGATTTGGCGGTCGTGAAAAAGGTAATGACCATGGCAGACAACTTCACCGTTAAAGATGTCAATGGCAACGTCGTGTTCAAAGTGAAAGGTTCCTTCTTAACCCTCCGTGATCGCCGTGTCTTGGTTGATGCTGCTGGTAACCCCATAACCACCCTACGTCGCAAGGTACTTTCAAATTTATTAATAAACTAATTTGCTTTCAGATTTTTATTTTAATGCTCAATATTTTAATTTTGTGATGATTTTTTTTTTGTATATACGATATTTTATGTTTTTTAAAAAATAACTAGTAGGACATCCGAGTATGGACTCACGTAGTTGTGCGTTGCACTTTATTTTTAGATAAGACGTAGCTTTATTACATTTAAATAATAATTAATATGAAAAACTGTATGTGTTATAAAACTATATTTTGTAACTAGAAACTCTCTATATATGAGTTTGTAAGTTAAAAATTATGCACTTATAATTTAGTTGATGAATAATTTAAGAAATCTTTTCAAAGAGAAATCTGATCTATTTTACTCAGAGCTCGTATATAAGTTATTCACAGTAAACTCAAAAAATTGTTTTGCTCAATGGACTCTTAATGTGTGAATTTCAACAATTGGTTGATTGAAACAAAAAAAAAAGTAAAAACATAAATGTTAGAGTAATGTTTATTGTTAAAAATTCATTATCCATTATACTTCATATATCCCAATAAAGTGTTGTTTAATGATTCTATTAAGTTGTATTATTGGTGCAGATAGTGACTATGCATGATCGGTGGGAAGCTTATAGGGGAGAAAGCACAAATTCTAAAGATCTTATATTTACATTGAGAAAATCATCCCTATTGCAGTTGAAGACCAAATTAGATGTGTTTCTAGCTGGTAATACCAAAGAAGATATTTGTGACTTTAAAGTCAAAGGTAGTTGGTTGGAACGATCTTGCATTATTTATGCAGGTGATACTAACAACATTGTTGCACAGGTAATTAAAAATCATATTCTAGTATATACAAGAGTATATAAGGTTTTACTTCCTCTTTTGTACCAAAAAAAATATAAGGTTTTACTTTGATTGGATTATAACTTAAGGACATATACTAATCATAAATCAATTGTTGTGATATTTTTGTTATTGCAGATGCACAAAAAGCACACTATTCAGAGTATTTTAATTGATAAAGATCATTTCGCAGTCACTGTTTATCCCAATGTTGATTACGCTTTCATAGTGGCATTAATTGCGATTCTTAACGAGATTAACGAAGATGATGATTGA

Protein sequence

>MS.gene42097.t1

MPAPTTTIFGSQYCAPYPVDLAVVKKVMTMADNFTVKDVNGNVVFKVKGSFLTLRDRRVLVDAAGNPITTLRRKIVTMHDRWEAYRGESTNSKDLIFTLRKSSLLQLKTKLDVFLAGNTKEDICDFKVKGSWLERSCIIYAGDTNNIVAQMHKKHTIQSILIDKDHFAVTVYPNVDYAFIVALIAILNEINEDDD