AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87040.t1 MTR_7g072120 92.166 217 11 2 1 216 1 212 2.18e-145 404
MS.gene87040.t1 MTR_7g072170 84.259 216 28 2 1 215 1 211 5.14e-133 373
MS.gene87040.t1 MTR_7g072140 82.407 216 34 1 1 216 1 212 1.06e-131 369
MS.gene87040.t1 MTR_7g072270 79.167 216 39 2 1 216 1 210 7.19e-127 357
MS.gene87040.t1 MTR_7g072250 69.869 229 49 5 2 215 17 240 2.47e-107 308
MS.gene87040.t1 MTR_7g072270 78.363 171 31 2 1 171 1 165 9.48e-98 281
MS.gene87040.t1 MTR_4g029430 61.458 192 68 3 24 214 12 198 4.76e-83 245
MS.gene87040.t1 MTR_7g071710 83.761 117 19 0 98 214 1 117 1.05e-72 216
MS.gene87040.t1 MTR_1g014180 54.497 189 75 6 25 208 3 185 1.87e-60 187
MS.gene87040.t1 MTR_1g014150 46.486 185 90 5 26 208 12 189 4.21e-47 154
MS.gene87040.t1 MTR_1g014160 37.668 223 112 8 4 208 27 240 3.04e-30 112
MS.gene87040.t1 MTR_5g074550 35.556 180 102 5 37 207 17 191 2.49e-26 100
MS.gene87040.t1 MTR_2g103840 30.303 198 124 6 20 213 17 204 7.45e-25 97.8
MS.gene87040.t1 MTR_4g010180 28.261 184 126 5 31 211 11 191 1.14e-22 91.7
MS.gene87040.t1 MTR_5g074560 31.214 173 109 5 37 207 20 184 1.02e-20 85.9
MS.gene87040.t1 MTR_2g103850 27.317 205 139 6 15 214 7 206 4.74e-20 85.1
MS.gene87040.t1 MTR_0650s0010 28.571 189 120 6 32 208 12 197 2.53e-18 80.1
MS.gene87040.t1 MTR_8g099000 28.723 188 114 7 40 209 45 230 1.44e-14 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87040.t1 AT5G01750 61.033 213 78 2 8 215 3 215 2.38e-101 292
MS.gene87040.t1 AT3G11740 65.285 193 65 2 26 216 2 194 1.94e-92 269
MS.gene87040.t1 AT2G14560 55.556 189 84 0 24 212 4 192 5.88e-78 233
MS.gene87040.t1 AT1G33840 52.381 210 98 2 1 209 1 209 1.49e-77 233
MS.gene87040.t1 AT2G14560 55.556 189 84 0 24 212 64 252 9.99e-77 232
MS.gene87040.t1 AT5G01750 59.412 170 64 2 8 172 3 172 4.00e-76 227
MS.gene87040.t1 AT2G14560 52.500 160 76 0 24 183 4 163 1.66e-62 192
MS.gene87040.t1 AT2G14560 52.500 160 76 0 24 183 64 223 2.58e-61 192
MS.gene87040.t1 AT1G33840 50.000 174 85 2 1 173 1 173 1.07e-60 188
MS.gene87040.t1 AT3G16900 41.935 186 87 4 27 211 15 180 1.39e-48 157
MS.gene87040.t1 AT3G56180 35.602 191 117 3 26 213 17 204 1.74e-38 132
MS.gene87040.t1 AT3G56180 34.574 188 116 3 26 210 17 200 3.42e-36 126
MS.gene87040.t1 AT3G10986 32.292 192 124 3 26 211 3 194 1.61e-31 114
MS.gene87040.t1 AT3G15810 30.508 177 117 3 40 214 33 205 2.11e-26 101
MS.gene87040.t1 AT1G80120 32.961 179 113 4 40 214 19 194 1.41e-20 86.3
MS.gene87040.t1 AT2G30270 30.000 180 112 6 30 207 12 179 8.75e-17 75.5
MS.gene87040.t1 AT2G38640 30.286 175 113 5 38 209 23 191 3.12e-16 74.3
MS.gene87040.t1 AT5G20640 24.873 197 131 5 27 208 12 206 6.17e-15 71.2
MS.gene87040.t1 AT2G05910 26.042 192 121 7 26 208 3 182 8.13e-15 70.5
MS.gene87040.t1 AT5G41590 22.222 216 153 5 9 209 3 218 9.03e-12 62.8

Find 54 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTCTTCATCTGGATTA+AGG 0.216511 7.4:+32402518 None:intergenic
AAAATCACAAACATCTTCTT+TGG 0.257604 7.4:+32402470 None:intergenic
TGATCGGTGGGAAGCTTATA+GGG 0.299737 7.4:-32402589 MS.gene87040:CDS
ATGATCGGTGGGAAGCTTAT+AGG 0.312103 7.4:-32402590 MS.gene87040:CDS
CATCTAATTTGGTCTTCATC+TGG 0.334921 7.4:+32402511 None:intergenic
GGATCTTGCCATCGTGAAAA+AGG 0.339002 7.4:-32403255 MS.gene87040:CDS
AGCTAGAAACACATCTAATT+TGG 0.366171 7.4:+32402500 None:intergenic
ACAGTATTGAGGACCGAATA+TGG 0.368124 7.4:+32403292 None:intergenic
GCAGATGGCTGTGGTTGATA+TGG 0.388244 7.4:+32403344 None:intergenic
CTTGCGACGGAGGGTGGTGA+TGG 0.396422 7.4:+32403109 None:intergenic
GAGGACCGAATATGGCGTTC+GGG 0.411432 7.4:+32403300 None:intergenic
GCAGCCCCGAACGCCATATT+CGG 0.412629 7.4:-32403305 MS.gene87040:CDS
ATCTGCATCTGCACCGATGC+AGG 0.415902 7.4:-32403327 MS.gene87040:CDS
TTCACGATGGCAAGATCCAC+TGG 0.437250 7.4:+32403260 None:intergenic
TTGCGACGGAGGGTGGTGAT+GGG 0.452287 7.4:+32403110 None:intergenic
TGAGGACCGAATATGGCGTT+CGG 0.452612 7.4:+32403299 None:intergenic
CGTGTCTTGCTTGATGCTGC+TGG 0.455360 7.4:-32403137 MS.gene87040:CDS
GCGTTCGGGGCTGCCTGCAT+CGG 0.455509 7.4:+32403314 None:intergenic
GGCGAAGTTTCCATCTGAAA+TGG 0.475827 7.4:+32403226 None:intergenic
CGATCTTGCATTGTTTATGC+TGG 0.480226 7.4:-32402423 MS.gene87040:CDS
AACATTGTCTTCAAAGTGAA+AGG 0.488864 7.4:-32403185 MS.gene87040:CDS
ATGAAAGCGTAGTCAACATT+GGG 0.498681 7.4:+32402192 None:intergenic
AAATTAGATGTGTTTCTAGC+TGG 0.501483 7.4:-32402498 MS.gene87040:CDS
TGATATGGCTGTGGTTGATA+AGG 0.507394 7.4:+32403359 None:intergenic
GATAAACCGTGACTGAGAAA+TGG 0.513280 7.4:+32402214 None:intergenic
CAAGCAAGACACGACGATCA+TGG 0.522499 7.4:+32403147 None:intergenic
TGTTGACTACGCTTTCATAG+TGG 0.527609 7.4:-32402187 MS.gene87040:CDS
ATCTAACCACATTGTTGCAC+AGG 0.530405 7.4:-32402397 MS.gene87040:intron
TATGAAAGCGTAGTCAACAT+TGG 0.542595 7.4:+32402191 None:intergenic
TAAAGTCAGAGGTAGTTGGT+TGG 0.551193 7.4:-32402448 MS.gene87040:CDS
TAAAGACCATTTCTCAGTCA+CGG 0.553557 7.4:-32402220 MS.gene87040:CDS
GGCTGTGGTTGATATGGCTG+TGG 0.558387 7.4:+32403350 None:intergenic
ATGGCAAGATCCACTGGATA+CGG 0.564173 7.4:+32403266 None:intergenic
TGCGACGGAGGGTGGTGATG+GGG 0.564704 7.4:+32403111 None:intergenic
CATCACCACCCTCCGTCGCA+AGG 0.565107 7.4:-32403108 MS.gene87040:intron
AAGGTGATGACCATTTCAGA+TGG 0.578907 7.4:-32403236 MS.gene87040:CDS
AGTGACTATGCATGATCGGT+GGG 0.580863 7.4:-32402601 MS.gene87040:CDS
GGTGCAGATGCAGATGGCTG+TGG 0.586855 7.4:+32403335 None:intergenic
GGATACGGAGCACAGTATTG+AGG 0.603779 7.4:+32403281 None:intergenic
TGCATCGGTGCAGATGCAGA+TGG 0.610431 7.4:+32403329 None:intergenic
TTGAACGTACCTTGCGACGG+AGG 0.612314 7.4:+32403099 None:intergenic
TAGTGACTATGCATGATCGG+TGG 0.614019 7.4:-32402602 MS.gene87040:CDS
TGAACGTACCTTGCGACGGA+GGG 0.617417 7.4:+32403100 None:intergenic
ACGTACCTTGCGACGGAGGG+TGG 0.620243 7.4:+32403103 None:intergenic
GATCGGTGGGAAGCTTATAG+GGG 0.629822 7.4:-32402588 MS.gene87040:CDS
AGGACCGAATATGGCGTTCG+GGG 0.642653 7.4:+32403301 None:intergenic
TAATTACCTGTGCAACAATG+TGG 0.644297 7.4:+32402391 None:intergenic
AGATAGTGACTATGCATGAT+CGG 0.644746 7.4:-32402605 MS.gene87040:CDS
ATACTGTGCTCCGTATCCAG+TGG 0.648223 7.4:-32403276 MS.gene87040:CDS
CAAGACACGACGATCATGGA+GGG 0.648557 7.4:+32403151 None:intergenic
GTTTCCGTTGACATCAGTGA+CGG 0.656884 7.4:+32403205 None:intergenic
TTCGCCGTCACTGATGTCAA+CGG 0.668229 7.4:-32403209 MS.gene87040:CDS
AAGTTGAACGTACCTTGCGA+CGG 0.684930 7.4:+32403096 None:intergenic
GCAAGACACGACGATCATGG+AGG 0.713270 7.4:+32403150 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCATTGTTTTATTTTATTT+TGG + chr7.4:32402639-32402658 None:intergenic 10.0%
!! AAAAATAAAAATGATCAATC+CGG + chr7.4:32402484-32402503 None:intergenic 15.0%
!! ACAAAAATACTGAAATTATT+AGG - chr7.4:32402515-32402534 MS.gene87040:CDS 15.0%
!!! AATAATTTCAGTATTTTTGT+AGG + chr7.4:32402515-32402534 None:intergenic 15.0%
!!! TTTTAATTAATTACGTTGAT+TGG - chr7.4:32403173-32403192 MS.gene87040:CDS 15.0%
!! AAAATAAAAATGATCAATCC+GGG + chr7.4:32402483-32402502 None:intergenic 20.0%
! AAAATCACAAACATCTTCTT+TGG + chr7.4:32403041-32403060 None:intergenic 25.0%
!! ATTTAACAGTAATGTTTTCC+CGG - chr7.4:32402462-32402481 MS.gene87040:CDS 25.0%
!! TAAGCAGAAATCAATTTTCA+TGG - chr7.4:32403211-32403230 MS.gene87040:CDS 25.0%
!!! ATCAACATTTTGTTTTGTGA+GGG - chr7.4:32402657-32402676 MS.gene87040:intron 25.0%
AACATTGTCTTCAAAGTGAA+AGG - chr7.4:32402323-32402342 MS.gene87040:intron 30.0%
AGCTAGAAACACATCTAATT+TGG + chr7.4:32403011-32403030 None:intergenic 30.0%
CTATCTGCTACACAAAATAA+TGG - chr7.4:32402603-32402622 MS.gene87040:CDS 30.0%
GGGAAAACATTACTGTTAAA+TGG + chr7.4:32402463-32402482 None:intergenic 30.0%
TACTGAAATTATTAGGTCGA+AGG - chr7.4:32402522-32402541 MS.gene87040:CDS 30.0%
TATACTCTTGTATACACAAG+AGG + chr7.4:32403148-32403167 None:intergenic 30.0%
! TACTGAATTTTGTCATGTTG+TGG - chr7.4:32402877-32402896 MS.gene87040:intron 30.0%
!! AAATTAGATGTGTTTCTAGC+TGG - chr7.4:32403010-32403029 MS.gene87040:intron 30.0%
!! CACTGTTACGAGTATTTTAA+TGG - chr7.4:32403264-32403283 MS.gene87040:CDS 30.0%
!!! ATTTTAAAGTCAGAGGTAGT+TGG - chr7.4:32403056-32403075 MS.gene87040:intron 30.0%
!!! GATCAACATTTTGTTTTGTG+AGG - chr7.4:32402656-32402675 MS.gene87040:intron 30.0%
!!! GTTTGTGATTTTAAAGTCAG+AGG - chr7.4:32403049-32403068 MS.gene87040:intron 30.0%
ACTATGAGCTCAACAATACA+TGG - chr7.4:32402711-32402730 MS.gene87040:intron 35.0%
AGATAGTGACTATGCATGAT+CGG - chr7.4:32402903-32402922 MS.gene87040:intron 35.0%
GTAACTAATACATGCTAGAG+AGG + chr7.4:32402778-32402797 None:intergenic 35.0%
TAAAGACCATTTCTCAGTCA+CGG - chr7.4:32403288-32403307 MS.gene87040:CDS 35.0%
TAATTACCTGTGCAACAATG+TGG + chr7.4:32403120-32403139 None:intergenic 35.0%
! ATGAAAGCGTAGTCAACATT+GGG + chr7.4:32403319-32403338 None:intergenic 35.0%
! CATCTAATTTGGTCTTCATC+TGG + chr7.4:32403000-32403019 None:intergenic 35.0%
! TATGAAAGCGTAGTCAACAT+TGG + chr7.4:32403320-32403339 None:intergenic 35.0%
! TTTGGTCTTCATCTGGATTA+AGG + chr7.4:32402993-32403012 None:intergenic 35.0%
AAGGTGATGACCATTTCAGA+TGG - chr7.4:32402272-32402291 MS.gene87040:intron 40.0%
ATCTAACCACATTGTTGCAC+AGG - chr7.4:32403111-32403130 MS.gene87040:CDS 40.0%
ATGAGCTCAACAATACATGG+CGG - chr7.4:32402714-32402733 MS.gene87040:intron 40.0%
CGATCTTGCATTGTTTATGC+TGG - chr7.4:32403085-32403104 MS.gene87040:intron 40.0%
GATAAACCGTGACTGAGAAA+TGG + chr7.4:32403297-32403316 None:intergenic 40.0%
TAAAGTCAGAGGTAGTTGGT+TGG - chr7.4:32403060-32403079 MS.gene87040:intron 40.0%
TTACTTCATTCGCTTGTCTC+AGG - chr7.4:32402794-32402813 MS.gene87040:intron 40.0%
! ACAGTATTGAGGACCGAATA+TGG + chr7.4:32402219-32402238 None:intergenic 40.0%
! TGATATGGCTGTGGTTGATA+AGG + chr7.4:32402152-32402171 None:intergenic 40.0%
! TGTTGACTACGCTTTCATAG+TGG - chr7.4:32403321-32403340 MS.gene87040:CDS 40.0%
AAGTTGAACGTACCTTGCGA+CGG + chr7.4:32402415-32402434 None:intergenic 45.0%
AGTGACTATGCATGATCGGT+GGG - chr7.4:32402907-32402926 MS.gene87040:intron 45.0%
ATGATCGGTGGGAAGCTTAT+AGG - chr7.4:32402918-32402937 MS.gene87040:intron 45.0%
ATGGCAAGATCCACTGGATA+CGG + chr7.4:32402245-32402264 None:intergenic 45.0%
GGATCTTGCCATCGTGAAAA+AGG - chr7.4:32402253-32402272 MS.gene87040:CDS 45.0%
GGCGAAGTTTCCATCTGAAA+TGG + chr7.4:32402285-32402304 None:intergenic 45.0%
GTTTCCGTTGACATCAGTGA+CGG + chr7.4:32402306-32402325 None:intergenic 45.0%
TAGTGACTATGCATGATCGG+TGG - chr7.4:32402906-32402925 MS.gene87040:intron 45.0%
TGATCGGTGGGAAGCTTATA+GGG - chr7.4:32402919-32402938 MS.gene87040:intron 45.0%
!! GGTCATCACCTTTTTCACGA+TGG + chr7.4:32402264-32402283 None:intergenic 45.0%
ATACTGTGCTCCGTATCCAG+TGG - chr7.4:32402232-32402251 MS.gene87040:CDS 50.0%
CAAGACACGACGATCATGGA+GGG + chr7.4:32402360-32402379 None:intergenic 50.0%
CAAGCAAGACACGACGATCA+TGG + chr7.4:32402364-32402383 None:intergenic 50.0%
GATCGGTGGGAAGCTTATAG+GGG - chr7.4:32402920-32402939 MS.gene87040:intron 50.0%
GGATACGGAGCACAGTATTG+AGG + chr7.4:32402230-32402249 None:intergenic 50.0%
TGAGGACCGAATATGGCGTT+CGG + chr7.4:32402212-32402231 None:intergenic 50.0%
TTCACGATGGCAAGATCCAC+TGG + chr7.4:32402251-32402270 None:intergenic 50.0%
TTCGCCGTCACTGATGTCAA+CGG - chr7.4:32402299-32402318 MS.gene87040:intron 50.0%
! GCAGATGGCTGTGGTTGATA+TGG + chr7.4:32402167-32402186 None:intergenic 50.0%
AGGACCGAATATGGCGTTCG+GGG + chr7.4:32402210-32402229 None:intergenic 55.0%
CGTGTCTTGCTTGATGCTGC+TGG - chr7.4:32402371-32402390 MS.gene87040:intron 55.0%
GAGGACCGAATATGGCGTTC+GGG + chr7.4:32402211-32402230 None:intergenic 55.0%
GCAAGACACGACGATCATGG+AGG + chr7.4:32402361-32402380 None:intergenic 55.0%
TGAACGTACCTTGCGACGGA+GGG + chr7.4:32402411-32402430 None:intergenic 55.0%
TTGAACGTACCTTGCGACGG+AGG + chr7.4:32402412-32402431 None:intergenic 55.0%
! ATCTGCATCTGCACCGATGC+AGG - chr7.4:32402181-32402200 MS.gene87040:CDS 55.0%
! GGCTGTGGTTGATATGGCTG+TGG + chr7.4:32402161-32402180 None:intergenic 55.0%
! TGCATCGGTGCAGATGCAGA+TGG + chr7.4:32402182-32402201 None:intergenic 55.0%
GCAGCCCCGAACGCCATATT+CGG - chr7.4:32402203-32402222 MS.gene87040:CDS 60.0%
GGTGCAGATGCAGATGGCTG+TGG + chr7.4:32402176-32402195 None:intergenic 60.0%
TTGCGACGGAGGGTGGTGAT+GGG + chr7.4:32402401-32402420 None:intergenic 60.0%
ACGTACCTTGCGACGGAGGG+TGG + chr7.4:32402408-32402427 None:intergenic 65.0%
CATCACCACCCTCCGTCGCA+AGG - chr7.4:32402400-32402419 MS.gene87040:CDS 65.0%
CTTGCGACGGAGGGTGGTGA+TGG + chr7.4:32402402-32402421 None:intergenic 65.0%
! TGCGACGGAGGGTGGTGATG+GGG + chr7.4:32402400-32402419 None:intergenic 65.0%
GCGTTCGGGGCTGCCTGCAT+CGG + chr7.4:32402197-32402216 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 32402140 32403390 32402140 ID=MS.gene87040
chr7.4 mRNA 32402140 32403390 32402140 ID=MS.gene87040.t1;Parent=MS.gene87040
chr7.4 exon 32403109 32403390 32403109 ID=MS.gene87040.t1.exon1;Parent=MS.gene87040.t1
chr7.4 CDS 32403109 32403390 32403109 ID=cds.MS.gene87040.t1;Parent=MS.gene87040.t1
chr7.4 exon 32402398 32402625 32402398 ID=MS.gene87040.t1.exon2;Parent=MS.gene87040.t1
chr7.4 CDS 32402398 32402625 32402398 ID=cds.MS.gene87040.t1;Parent=MS.gene87040.t1
chr7.4 exon 32402140 32402280 32402140 ID=MS.gene87040.t1.exon3;Parent=MS.gene87040.t1
chr7.4 CDS 32402140 32402280 32402140 ID=cds.MS.gene87040.t1;Parent=MS.gene87040.t1
Gene Sequence

>MS.gene87040

ATGGCTTATCCTTATCAACCACAGCCATATCAACCACAGCCATCTGCATCTGCACCGATGCAGGCAGCCCCGAACGCCATATTCGGTCCTCAATACTGTGCTCCGTATCCAGTGGATCTTGCCATCGTGAAAAAGGTGATGACCATTTCAGATGGAAACTTCGCCGTCACTGATGTCAACGGAAACATTGTCTTCAAAGTGAAAGGTTCTCTCTTAACCCTCCATGATCGTCGTGTCTTGCTTGATGCTGCTGGTAACCCCATCACCACCCTCCGTCGCAAGGTACGTTCAACTTTATTCTCTCTTTTACGAATTTTCTTCCATTTAACAGTAATGTTTTCCCGGATTGATCATTTTTATTTTTTAAATCAACCTACAAAAATACTGAAATTATTAGGTCGAAGGTGATGATCTGAGTTTGAATCTCGAATCAACTTTTCAATTGTTATTATTGTTATTTCGTCTATCTGCTACACAAAATAATGGTCTCATTGTACCAAAATAAAATAAAACAATGATCAACATTTTGTTTTGTGAGGGAAACAAAGCTTACAACATTTCATTTGATATCACTATGAGCTCAACAATACATGGCGGTACATCAACAGAAATATGAAGAGTAGTTAAGAATGTGACCTCTCTAGCATGTATTAGTTACTTCATTCGCTTGTCTCAGGCTAAACTCGACATGAGAGTTTGTGAAAAATAAATTACAACGCTTGTTTCATCTAATATAGTACTGAATTTTGTCATGTTGTGGTGCAGATAGTGACTATGCATGATCGGTGGGAAGCTTATAGGGGTGAAAGCACAGATTCTAAAGATCTAATATTTACATTGAAGAGATCATCCTTAATCCAGATGAAGACCAAATTAGATGTGTTTCTAGCTGGTAATACCAAAGAAGATGTTTGTGATTTTAAAGTCAGAGGTAGTTGGTTGGAACGATCTTGCATTGTTTATGCTGGTGAATCTAACCACATTGTTGCACAGGTAATTAAAAATCCTCTTGTGTATACAAGAGTATATTTAGTTTTAATTAATTACGTTGATTGGATTATAAGTATATACTAAGCAGAAATCAATTTTCATGGCATTGTTGTTATGCAGATGCACAAAAAGCACACTGTTACGAGTATTTTAATGGATAAAGACCATTTCTCAGTCACGGTTTATCCCAATGTTGACTACGCTTTCATAGTGGCATTAATCGTGATTCTTGATGAGACTAACGAAGATGAAAAAGATTGA

Protein sequence

>MS.gene87040.t1

MAYPYQPQPYQPQPSASAPMQAAPNAIFGPQYCAPYPVDLAIVKKVMTISDGNFAVTDVNGNIVFKVKGSLLTLHDRRVLLDAAGNPITTLRRKIVTMHDRWEAYRGESTDSKDLIFTLKRSSLIQMKTKLDVFLAGNTKEDVCDFKVRGSWLERSCIVYAGESNHIVAQMHKKHTVTSILMDKDHFSVTVYPNVDYAFIVALIVILDETNEDEKD