AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87769


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87769.t1 MTR_7g072120 78.095 210 38 4 1 202 1 210 1.32e-118 335
MS.gene87769.t1 MTR_7g072270 75.845 207 44 1 1 201 1 207 4.81e-117 331
MS.gene87769.t1 MTR_7g072170 74.762 210 45 2 1 202 1 210 1.16e-113 323
MS.gene87769.t1 MTR_7g072140 73.810 210 47 2 1 202 1 210 1.85e-112 320
MS.gene87769.t1 MTR_7g072250 68.137 204 51 2 7 200 38 237 1.88e-96 280
MS.gene87769.t1 MTR_7g072270 75.152 165 35 1 1 159 1 165 1.97e-90 262
MS.gene87769.t1 MTR_7g071710 93.162 117 8 0 86 202 1 117 5.28e-78 229
MS.gene87769.t1 MTR_4g029430 56.684 187 76 2 16 201 15 197 6.14e-75 224
MS.gene87769.t1 MTR_1g014180 53.226 186 77 5 16 196 5 185 7.59e-61 188
MS.gene87769.t1 MTR_1g014150 45.361 194 97 5 4 195 2 188 3.30e-47 154
MS.gene87769.t1 MTR_1g014160 40.426 188 87 6 26 195 59 239 1.14e-29 110
MS.gene87769.t1 MTR_2g103840 29.940 167 109 5 39 201 42 204 3.36e-20 85.1
MS.gene87769.t1 MTR_5g074550 30.000 180 111 6 26 195 17 191 7.94e-18 78.2
MS.gene87769.t1 MTR_2g103850 27.273 187 127 6 21 202 24 206 1.05e-15 73.2
MS.gene87769.t1 MTR_4g010180 24.599 187 134 6 20 202 11 194 1.85e-15 72.0
MS.gene87769.t1 MTR_5g074560 26.590 173 116 6 26 195 20 184 1.20e-12 63.9
MS.gene87769.t1 MTR_0650s0010 26.984 189 126 6 21 198 12 199 1.91e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87769.t1 AT5G01750 56.000 200 83 2 8 202 15 214 1.11e-85 252
MS.gene87769.t1 AT3G11740 56.842 190 80 2 15 202 2 191 6.08e-78 232
MS.gene87769.t1 AT2G14560 50.538 186 91 1 16 200 7 192 4.24e-66 202
MS.gene87769.t1 AT2G14560 48.241 199 101 2 3 200 55 252 3.92e-65 202
MS.gene87769.t1 AT5G01750 55.696 158 65 2 8 160 15 172 5.65e-63 193
MS.gene87769.t1 AT1G33840 48.619 181 92 1 20 199 31 211 3.41e-62 193
MS.gene87769.t1 AT2G14560 50.340 147 72 1 16 161 7 153 2.47e-51 164
MS.gene87769.t1 AT2G14560 47.500 160 82 2 3 161 55 213 1.84e-50 163
MS.gene87769.t1 AT1G33840 46.853 143 75 1 20 161 31 173 2.26e-46 151
MS.gene87769.t1 AT3G16900 42.162 185 87 4 16 199 15 180 4.07e-46 150
MS.gene87769.t1 AT3G56180 34.536 194 120 4 12 201 14 204 5.21e-34 120
MS.gene87769.t1 AT3G56180 34.021 194 119 5 12 201 14 202 9.46e-32 114
MS.gene87769.t1 AT3G10986 32.632 190 121 4 15 197 3 192 5.59e-30 109
MS.gene87769.t1 AT3G15810 27.711 166 114 3 39 202 44 205 1.18e-20 86.3
MS.gene87769.t1 AT1G80120 31.361 169 109 4 39 203 30 195 4.96e-17 76.3
MS.gene87769.t1 AT2G38640 29.586 169 110 5 33 198 30 192 7.32e-13 64.7
MS.gene87769.t1 AT2G05910 28.994 169 99 7 39 198 28 184 7.71e-13 64.7
MS.gene87769.t1 AT5G20640 27.072 181 115 6 36 201 33 211 7.69e-11 59.7

Find 45 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACAAACATCTTCTT+TGG 0.257604 7.1:+29944632 None:intergenic
CTTGCGACGTAGGGTGGTTA+TGG 0.262386 7.1:+29945377 None:intergenic
GGATTTGGCGGTCGTCAAAA+AGG 0.341778 7.1:-29945520 MS.gene87769:CDS
TTGCGACGTAGGGTGGTTAT+GGG 0.360381 7.1:+29945378 None:intergenic
ACAATATTGAGAGCCGAATA+TGG 0.380388 7.1:+29945557 None:intergenic
GTTGTCATTGACATCTTTAA+CGG 0.384141 7.1:+29945473 None:intergenic
TGTTCCGTATCCGGTGGATT+TGG 0.392757 7.1:-29945535 MS.gene87769:CDS
AGCTAGAAACACATCTAGTT+TGG 0.401309 7.1:+29944662 None:intergenic
TCAATATTGTGTTCCGTATC+CGG 0.406094 7.1:-29945544 MS.gene87769:CDS
GGGGTGGGTGCTGGCATCGG+TGG 0.406707 7.1:+29945582 None:intergenic
AAAGTCAAAGGTAGTTGGTT+GGG 0.414389 7.1:-29944609 MS.gene87769:CDS
CAATCTTGTATTGTTTATGC+TGG 0.465716 7.1:-29944585 MS.gene87769:CDS
ACCGCCAAATCCACCGGATA+CGG 0.483412 7.1:+29945531 None:intergenic
TTGACGACCGCCAAATCCAC+CGG 0.483926 7.1:+29945525 None:intergenic
GATAAACAGTGACCGCGAAA+TGG 0.505710 7.1:+29944388 None:intergenic
TAAAGTCAAAGGTAGTTGGT+TGG 0.516280 7.1:-29944610 MS.gene87769:CDS
TCCGTATCCGGTGGATTTGG+CGG 0.516653 7.1:-29945532 MS.gene87769:CDS
CCTCTGTGACCACCGTGTCT+TGG 0.523038 7.1:-29945418 MS.gene87769:CDS
AAACTAGATGTGTTTCTAGC+TGG 0.525823 7.1:-29944660 MS.gene87769:CDS
AACGGTGAAGTTGTCTGCCA+TGG 0.529412 7.1:+29945491 None:intergenic
AATTGATCAAGACCATTTCG+CGG 0.543054 7.1:-29944400 MS.gene87769:CDS
TGAAAGTACCTTGCGACGTA+GGG 0.546082 7.1:+29945368 None:intergenic
CGTGTCTTGGTTGATGCTGT+TGG 0.551048 7.1:-29945405 MS.gene87769:CDS
TGTTGATTACGCTTTCATAG+TGG 0.551491 7.1:-29944361 MS.gene87769:CDS
TATTGAGAGCCGAATATGGT+GGG 0.560893 7.1:+29945561 None:intergenic
TAGTAACAACATTGTTGCAC+AGG 0.564856 7.1:-29944559 MS.gene87769:intron
TTGAAAGTACCTTGCGACGT+AGG 0.577009 7.1:+29945367 None:intergenic
AGTGAGTATGCATGATCGGT+GGG 0.580863 7.1:-29944763 MS.gene87769:CDS
TATGAAAGCGTAATCAACAC+TGG 0.595693 7.1:+29944365 None:intergenic
CATAACCACCCTACGTCGCA+AGG 0.600007 7.1:-29945376 MS.gene87769:intron
AAGTACCTTGCGACGTAGGG+TGG 0.605892 7.1:+29945371 None:intergenic
ATATTGTGTTCCGTATCCGG+TGG 0.613508 7.1:-29945541 MS.gene87769:CDS
TAGTGAGTATGCATGATCGG+TGG 0.614019 7.1:-29944764 MS.gene87769:CDS
ATTGAGAGCCGAATATGGTG+GGG 0.617660 7.1:+29945562 None:intergenic
AACGTCGTGTTCAAAGTGAA+AGG 0.621566 7.1:-29945453 MS.gene87769:CDS
TTGAGAGCCGAATATGGTGG+GGG 0.626700 7.1:+29945563 None:intergenic
AGATAGTGAGTATGCATGAT+CGG 0.635485 7.1:-29944767 MS.gene87769:CDS
CAAGACACGGTGGTCACAGA+GGG 0.636102 7.1:+29945419 None:intergenic
ATGAAAGCGTAATCAACACT+GGG 0.644614 7.1:+29944366 None:intergenic
TGCGACGTAGGGTGGTTATG+GGG 0.653394 7.1:+29945379 None:intergenic
CCAAGACACGGTGGTCACAG+AGG 0.661785 7.1:+29945418 None:intergenic
ATATTGAGAGCCGAATATGG+TGG 0.662337 7.1:+29945560 None:intergenic
CAACAGCATCAACCAAGACA+CGG 0.674118 7.1:+29945406 None:intergenic
GATCGGTGGGAAGCTTACAG+AGG 0.712766 7.1:-29944750 MS.gene87769:CDS
CAGCATCAACCAAGACACGG+TGG 0.792973 7.1:+29945409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTTTCTTTTTTTTC+TGG + chr7.1:29945026-29945045 None:intergenic 10.0%
!!! AAAAAAATTATTTTGCTCAA+TGG - chr7.1:29944958-29944977 MS.gene87769:intron 15.0%
!!! ATATAATGTTTTACATTGAT+TGG - chr7.1:29945411-29945430 MS.gene87769:CDS 15.0%
!!! TTTTATAAAACATAACTAGT+AGG - chr7.1:29944665-29944684 MS.gene87769:CDS 15.0%
!! TTGAGATATATGAAGTATAA+TGG + chr7.1:29945088-29945107 None:intergenic 20.0%
!!! GATTCATTAAGTTTTACTAT+TGG - chr7.1:29945124-29945143 MS.gene87769:intron 20.0%
! AAAATCACAAACATCTTCTT+TGG + chr7.1:29945288-29945307 None:intergenic 25.0%
! ATACTCTTGTATATACTAGA+TGG + chr7.1:29945393-29945412 None:intergenic 25.0%
! ATCATCTACTAAATTATGAG+TGG + chr7.1:29944858-29944877 None:intergenic 25.0%
! ATTGATTGGATTATCACTTA+AGG - chr7.1:29945425-29945444 MS.gene87769:CDS 25.0%
!!! ATTTTAAAGTCAAAGGTAGT+TGG - chr7.1:29945303-29945322 MS.gene87769:intron 25.0%
!!! GTTTGTGATTTTAAAGTCAA+AGG - chr7.1:29945296-29945315 MS.gene87769:intron 25.0%
CAATCTTGTATTGTTTATGC+TGG - chr7.1:29945332-29945351 MS.gene87769:intron 30.0%
GTTGTCATTGACATCTTTAA+CGG + chr7.1:29944447-29944466 None:intergenic 30.0%
AAAGTCAAAGGTAGTTGGTT+GGG - chr7.1:29945308-29945327 MS.gene87769:intron 35.0%
AATTGATCAAGACCATTTCG+CGG - chr7.1:29945517-29945536 MS.gene87769:CDS 35.0%
ACAATATTGAGAGCCGAATA+TGG + chr7.1:29944363-29944382 None:intergenic 35.0%
AGATAGTGAGTATGCATGAT+CGG - chr7.1:29945150-29945169 MS.gene87769:intron 35.0%
AGCTAGAAACACATCTAGTT+TGG + chr7.1:29945258-29945277 None:intergenic 35.0%
TAAAGTCAAAGGTAGTTGGT+TGG - chr7.1:29945307-29945326 MS.gene87769:intron 35.0%
TAGTAACAACATTGTTGCAC+AGG - chr7.1:29945358-29945377 MS.gene87769:intron 35.0%
TCAATATTGTGTTCCGTATC+CGG - chr7.1:29944373-29944392 MS.gene87769:CDS 35.0%
! ATGAAAGCGTAATCAACACT+GGG + chr7.1:29945554-29945573 None:intergenic 35.0%
! TATGAAAGCGTAATCAACAC+TGG + chr7.1:29945555-29945574 None:intergenic 35.0%
! TGTTGATTACGCTTTCATAG+TGG - chr7.1:29945556-29945575 MS.gene87769:CDS 35.0%
!! AAACTAGATGTGTTTCTAGC+TGG - chr7.1:29945257-29945276 MS.gene87769:intron 35.0%
AACGTCGTGTTCAAAGTGAA+AGG - chr7.1:29944464-29944483 MS.gene87769:intron 40.0%
ATATTGAGAGCCGAATATGG+TGG + chr7.1:29944360-29944379 None:intergenic 40.0%
CGTCAAAAAGGTAATGACCA+TGG - chr7.1:29944409-29944428 MS.gene87769:CDS 40.0%
TATTGAGAGCCGAATATGGT+GGG + chr7.1:29944359-29944378 None:intergenic 40.0%
AGTGAGTATGCATGATCGGT+GGG - chr7.1:29945154-29945173 MS.gene87769:intron 45.0%
ATATTGTGTTCCGTATCCGG+TGG - chr7.1:29944376-29944395 MS.gene87769:CDS 45.0%
ATTGAGAGCCGAATATGGTG+GGG + chr7.1:29944358-29944377 None:intergenic 45.0%
CAACAGCATCAACCAAGACA+CGG + chr7.1:29944514-29944533 None:intergenic 45.0%
GATAAACAGTGACCGCGAAA+TGG + chr7.1:29945532-29945551 None:intergenic 45.0%
TAGTGAGTATGCATGATCGG+TGG - chr7.1:29945153-29945172 MS.gene87769:intron 45.0%
!! TGAAAGTACCTTGCGACGTA+GGG + chr7.1:29944552-29944571 None:intergenic 45.0%
!! TTGAAAGTACCTTGCGACGT+AGG + chr7.1:29944553-29944572 None:intergenic 45.0%
AACGGTGAAGTTGTCTGCCA+TGG + chr7.1:29944429-29944448 None:intergenic 50.0%
ACTAGTAGGACATCCGAGTG+TGG - chr7.1:29944679-29944698 MS.gene87769:CDS 50.0%
CAACTACGTGAGTCCACACT+CGG + chr7.1:29944695-29944714 None:intergenic 50.0%
GGATTTGGCGGTCGTCAAAA+AGG - chr7.1:29944397-29944416 MS.gene87769:CDS 50.0%
TTGAGAGCCGAATATGGTGG+GGG + chr7.1:29944357-29944376 None:intergenic 50.0%
TTGCGACGTAGGGTGGTTAT+GGG + chr7.1:29944542-29944561 None:intergenic 50.0%
! CGTGTCTTGGTTGATGCTGT+TGG - chr7.1:29944512-29944531 MS.gene87769:intron 50.0%
! TGTTCCGTATCCGGTGGATT+TGG - chr7.1:29944382-29944401 MS.gene87769:CDS 50.0%
AAGTACCTTGCGACGTAGGG+TGG + chr7.1:29944549-29944568 None:intergenic 55.0%
ACCGCCAAATCCACCGGATA+CGG + chr7.1:29944389-29944408 None:intergenic 55.0%
CAAGACACGGTGGTCACAGA+GGG + chr7.1:29944501-29944520 None:intergenic 55.0%
CAGCATCAACCAAGACACGG+TGG + chr7.1:29944511-29944530 None:intergenic 55.0%
CATAACCACCCTACGTCGCA+AGG - chr7.1:29944541-29944560 MS.gene87769:intron 55.0%
CTTGCGACGTAGGGTGGTTA+TGG + chr7.1:29944543-29944562 None:intergenic 55.0%
GATCGGTGGGAAGCTTACAG+AGG - chr7.1:29945167-29945186 MS.gene87769:intron 55.0%
TGCGACGTAGGGTGGTTATG+GGG + chr7.1:29944541-29944560 None:intergenic 55.0%
TTGACGACCGCCAAATCCAC+CGG + chr7.1:29944395-29944414 None:intergenic 55.0%
! TCCGTATCCGGTGGATTTGG+CGG - chr7.1:29944385-29944404 MS.gene87769:CDS 55.0%
CCAAGACACGGTGGTCACAG+AGG + chr7.1:29944502-29944521 None:intergenic 60.0%
CCTCTGTGACCACCGTGTCT+TGG - chr7.1:29944499-29944518 MS.gene87769:intron 60.0%
GCACCCACCCCCACCATATT+CGG - chr7.1:29944347-29944366 MS.gene87769:CDS 60.0%
! AATATGGTGGGGGTGGGTGC+TGG + chr7.1:29944347-29944366 None:intergenic 60.0%
! AGAGCCGAATATGGTGGGGG+TGG + chr7.1:29944354-29944373 None:intergenic 60.0%
! GAGCCGAATATGGTGGGGGT+GGG + chr7.1:29944353-29944372 None:intergenic 60.0%
!! GTGGGGGTGGGTGCTGGCAT+CGG + chr7.1:29944341-29944360 None:intergenic 70.0%
!! GGGGTGGGTGCTGGCATCGG+TGG + chr7.1:29944338-29944357 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 29944317 29945622 29944317 ID=MS.gene87769
chr7.1 mRNA 29944317 29945622 29944317 ID=MS.gene87769.t1;Parent=MS.gene87769
chr7.1 exon 29945377 29945622 29945377 ID=MS.gene87769.t1.exon1;Parent=MS.gene87769.t1
chr7.1 CDS 29945377 29945622 29945377 ID=cds.MS.gene87769.t1;Parent=MS.gene87769.t1
chr7.1 exon 29944560 29944787 29944560 ID=MS.gene87769.t1.exon2;Parent=MS.gene87769.t1
chr7.1 CDS 29944560 29944787 29944560 ID=cds.MS.gene87769.t1;Parent=MS.gene87769.t1
chr7.1 exon 29944317 29944454 29944317 ID=MS.gene87769.t1.exon3;Parent=MS.gene87769.t1
chr7.1 CDS 29944317 29944454 29944317 ID=cds.MS.gene87769.t1;Parent=MS.gene87769.t1
Gene Sequence

>MS.gene87769

ATGTCTTATTCTTATCAACCACCGATGCCAGCACCCACCCCCACCATATTCGGCTCTCAATATTGTGTTCCGTATCCGGTGGATTTGGCGGTCGTCAAAAAGGTAATGACCATGGCAGACAACTTCACCGTTAAAGATGTCAATGACAACGTCGTGTTCAAAGTGAAAGGTTCTCTCTTAACCCTCTGTGACCACCGTGTCTTGGTTGATGCTGTTGGTAACCCCATAACCACCCTACGTCGCAAGGTACTTTCAACTTTATTAAGAAACTAGTTTTCTTTCAGACTTTTATTTTAATGCTCAATATTTTATTTTTGTGATGAATTTTTTTATATATACGATATTTTATTTTATAAAACATAACTAGTAGGACATCCGAGTGTGGACTCACGTAGTTGTGCGTTGCACTTTATTTTTAGATAAGACGCAGCTTTATTACATTTCAATAATATTTAATATGAAAAATTGTATGTGTTATAAATTTATATATTTTGTAACTTGAAACTCTTTACATAAAAGTTTGTAAGTTAAAAATTATCCACTCATAATTTAGTAGATGATTAATTTTTGAAATCTTTTCAAAGAGAAATATGATATATTTTACTCAGAGCTCATATATAAGTTATTCACTATAAACTTAAAAAAAAATTATTTTGCTCAATGGAGTCTTATTAGTGTGTGAATTTCAATAATTCGTTGATTGAAACCAGAAAAAAAAGAAAAAATATAAATGTTAGAGTAACGTTTATTGTTAAAAAATCATATTATCCATTATACTTCATATATCTCAATAAAGTGTTGTTTAATGATTCATTAAGTTTTACTATTGGTGCAGATAGTGAGTATGCATGATCGGTGGGAAGCTTACAGAGGAGAAAGCACAAATTCGAAAGATCTTATATTTACATTGAGAAAATCATCGCTATTGCAGTTGAAGACCAAACTAGATGTGTTTCTAGCTGGTAATACCAAAGAAGATGTTTGTGATTTTAAAGTCAAAGGTAGTTGGTTGGGACAATCTTGTATTGTTTATGCTGGTGATAGTAACAACATTGTTGCACAGGTAGTAAAAACCATCTAGTATATACAAGAGTATATAATGTTTTACATTGATTGGATTATCACTTAAGGACATATACTAATCATAAATCAATTTTTGTTATTGCAGATGCACAAAAAGCACACTATTAAGAGTATTTTAATTGATCAAGACCATTTCGCGGTCACTGTTTATCCCAGTGTTGATTACGCTTTCATAGTGGCATTAATCGCGATTCTTAACGAGATTAACGAAGATGATGTTTGA

Protein sequence

>MS.gene87769.t1

MSYSYQPPMPAPTPTIFGSQYCVPYPVDLAVVKKVMTMADNFTVKDVNDNVVFKVKGSLLTLCDHRVLVDAVGNPITTLRRKIVSMHDRWEAYRGESTNSKDLIFTLRKSSLLQLKTKLDVFLAGNTKEDVCDFKVKGSWLGQSCIVYAGDSNNIVAQMHKKHTIKSILIDQDHFAVTVYPSVDYAFIVALIAILNEINEDDV