AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017326.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017326.01.T02 MTR_3g105340 96.330 109 4 0 1 109 10 118 1.16e-76 222
MsG0380017326.01.T02 MTR_1g014930 53.509 114 46 2 3 109 17 130 6.43e-36 120
MsG0380017326.01.T02 MTR_8g021190 55.914 93 39 1 2 92 30 122 7.25e-34 115
MsG0380017326.01.T02 MTR_4g125030 55.102 98 42 1 1 96 21 118 8.04e-32 108
MsG0380017326.01.T02 MTR_4g125010 48.182 110 51 3 1 106 23 130 1.86e-27 99.0
MsG0380017326.01.T02 MTR_5g006430 47.297 74 38 1 1 73 1 74 1.70e-22 84.7
MsG0380017326.01.T02 MTR_8g013990 57.143 63 26 1 2 64 62 123 1.36e-20 82.0
MsG0380017326.01.T02 MTR_3g109860 36.111 108 68 1 2 108 36 143 4.45e-20 79.7
MsG0380017326.01.T02 MTR_2g101140 58.065 62 25 1 2 63 62 122 9.23e-20 80.1
MsG0380017326.01.T02 MTR_1g103550 45.333 75 39 2 1 73 1 75 1.77e-19 77.4
MsG0380017326.01.T01 MTR_3g105340 94.915 118 6 0 1 118 1 118 4.22e-82 236
MsG0380017326.01.T01 MTR_1g014930 53.509 114 46 2 12 118 17 130 4.04e-36 120
MsG0380017326.01.T01 MTR_8g021190 55.208 96 41 1 8 101 27 122 3.28e-34 116
MsG0380017326.01.T01 MTR_4g125030 55.102 98 42 1 10 105 21 118 6.18e-32 109
MsG0380017326.01.T01 MTR_4g125010 46.957 115 55 3 5 115 18 130 5.80e-28 100
MsG0380017326.01.T01 MTR_5g006430 47.297 74 38 1 10 82 1 74 1.55e-22 85.1
MsG0380017326.01.T01 MTR_2g101140 52.778 72 33 1 1 72 52 122 8.20e-21 83.2
MsG0380017326.01.T01 MTR_8g013990 57.143 63 26 1 11 73 62 123 1.19e-20 82.8
MsG0380017326.01.T01 MTR_3g109860 36.111 108 68 1 11 117 36 143 6.49e-20 79.7
MsG0380017326.01.T01 MTR_1g103550 45.946 74 38 2 10 81 1 74 1.36e-19 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017326.01.T02 AT1G72510 57.447 94 38 1 5 96 36 129 2.13e-34 116
MsG0380017326.01.T02 AT1G72510 57.447 94 38 1 5 96 36 129 2.13e-34 116
MsG0380017326.01.T02 AT2G09970 48.980 98 47 2 2 96 33 130 1.69e-27 99.4
MsG0380017326.01.T02 AT5G20670 47.959 98 46 3 3 97 23 118 4.18e-23 87.8
MsG0380017326.01.T02 AT1G54095 46.809 94 46 2 2 92 15 107 1.20e-20 81.3
MsG0380017326.01.T02 AT4G14819 36.697 109 62 3 1 109 1 102 2.50e-19 76.6
MsG0380017326.01.T02 AT3G22540 38.462 91 51 3 1 90 1 87 6.67e-18 73.2
MsG0380017326.01.T02 AT1G79770 45.263 95 44 3 1 91 50 140 1.06e-15 68.9
MsG0380017326.01.T02 AT5G25840 46.479 71 27 2 2 64 42 109 3.60e-13 62.4
MsG0380017326.01.T02 AT2G25780 38.202 89 51 3 6 91 39 126 1.22e-12 60.8
MsG0380017326.01.T01 AT1G72510 57.447 94 38 1 14 105 36 129 1.72e-34 117
MsG0380017326.01.T01 AT1G72510 57.447 94 38 1 14 105 36 129 1.72e-34 117
MsG0380017326.01.T01 AT2G09970 48.980 98 47 2 11 105 33 130 1.56e-27 99.8
MsG0380017326.01.T01 AT5G20670 47.959 98 46 3 12 106 23 118 3.15e-23 88.2
MsG0380017326.01.T01 AT1G54095 45.192 104 52 3 1 101 6 107 1.88e-21 83.6
MsG0380017326.01.T01 AT4G14819 36.697 109 62 3 10 118 1 102 2.71e-19 77.0
MsG0380017326.01.T01 AT3G22540 38.462 91 51 3 10 99 1 87 5.55e-18 73.6
MsG0380017326.01.T01 AT1G79770 43.878 98 47 3 7 100 47 140 8.44e-16 69.7
MsG0380017326.01.T01 AT5G25840 42.391 92 38 3 3 82 34 122 1.07e-13 64.3
MsG0380017326.01.T01 AT2G25780 38.202 89 51 3 15 100 39 126 1.49e-12 60.8

Find 30 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAACCGTGGGTTTATT+AGG 0.262331 3:+94979589 None:intergenic
CTCCATCGTCGCCGCGGTTT+CGG 0.297193 3:+94979822 None:intergenic
TTTCAAAGCTTCTTCTTTCA+CGG 0.372656 3:+94979684 None:intergenic
TACCTGGGTCGTTGGATTTG+TGG 0.383506 3:-94979721 MsG0380017326.01.T01:CDS
ACCTGGGTCGTTGGATTTGT+GGG 0.391134 3:-94979720 MsG0380017326.01.T01:CDS
GTGCTACTGTTGCGGCTTAA+CGG 0.404880 3:-94979785 MsG0380017326.01.T01:CDS
AAACTCAAAGGTTGTTTCCT+CGG 0.484002 3:+94979523 None:intergenic
CCATGTTCGTGAGAAATACC+TGG 0.494919 3:-94979737 MsG0380017326.01.T01:CDS
GCCGCAACAGTAGCACTTCA+CGG 0.509628 3:+94979792 None:intergenic
GTTGGTAGATCGCATAGATA+AGG 0.511238 3:-94979493 None:intergenic
GTGAGAAATACCTGGGTCGT+TGG 0.543743 3:-94979729 MsG0380017326.01.T01:CDS
GTGTTGATTAAGAGCTTCAT+CGG 0.550067 3:+94979642 None:intergenic
AACCGCGGCGACGATGGAGG+TGG 0.571492 3:-94979818 MsG0380017326.01.T01:CDS
AACCGAAACCGCGGCGACGA+TGG 0.582114 3:-94979824 MsG0380017326.01.T01:CDS
TCAACCGTGGGTTTATTAGG+AGG 0.587396 3:+94979592 None:intergenic
CCAGGTATTTCTCACGAACA+TGG 0.611108 3:+94979737 None:intergenic
TTCGGTCTTTGGATTCTCCG+AGG 0.613420 3:-94979540 MsG0380017326.01.T01:CDS
CGAAACCGCGGCGACGATGG+AGG 0.615356 3:-94979821 MsG0380017326.01.T01:CDS
AGCAAGAATCAAATCAACCG+TGG 0.629275 3:+94979579 None:intergenic
GCCCACAAATCCAACGACCC+AGG 0.634250 3:+94979719 None:intergenic
CCAACCGTTCGAAAACTCAA+AGG 0.637318 3:+94979511 None:intergenic
CATGTTCGTGAGAAATACCT+GGG 0.642264 3:-94979736 MsG0380017326.01.T01:CDS
GCAAGAATCAAATCAACCGT+GGG 0.653690 3:+94979580 None:intergenic
GATTTGTGGGCTATGTGCAG+AGG 0.661005 3:-94979707 MsG0380017326.01.T01:CDS
GCCGTGAAGTGCTACTGTTG+CGG 0.673482 3:-94979793 MsG0380017326.01.T01:CDS
AAGTCCTCCTAATAAACCCA+CGG 0.676099 3:-94979596 MsG0380017326.01.T01:CDS
CTACTGTTGCGGCTTAACGG+AGG 0.677501 3:-94979782 MsG0380017326.01.T01:CDS
TACCACCTCCATCGTCGCCG+CGG 0.680469 3:+94979816 None:intergenic
GATGGCTTTAACCGAAACCG+CGG 0.711918 3:-94979833 MsG0380017326.01.T01:CDS
CGAACATGGTCAATATACGA+CGG 0.745897 3:+94979751 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTTCAAAGCTTCTTCTTTCA+CGG + Chr3:94979646-94979665 None:intergenic 30.0%
! CTGTGAAACATCTTCTTTTT+CGG - Chr3:94979769-94979788 MsG0380017326.01.T01:CDS 30.0%
AAATCAACCGTGGGTTTATT+AGG + Chr3:94979741-94979760 None:intergenic 35.0%
! AAACTCAAAGGTTGTTTCCT+CGG + Chr3:94979807-94979826 None:intergenic 35.0%
!! ACAACCTTTGAGTTTTCGAA+CGG - Chr3:94979812-94979831 MsG0380017326.01.T01:CDS 35.0%
!! GTGTTGATTAAGAGCTTCAT+CGG + Chr3:94979688-94979707 None:intergenic 35.0%
!!! ACATCTTCTTTTTCGGTCTT+TGG - Chr3:94979776-94979795 MsG0380017326.01.T01:CDS 35.0%
AAGTCCTCCTAATAAACCCA+CGG - Chr3:94979731-94979750 MsG0380017326.01.T01:CDS 40.0%
AGCAAGAATCAAATCAACCG+TGG + Chr3:94979751-94979770 None:intergenic 40.0%
CATGTTCGTGAGAAATACCT+GGG - Chr3:94979591-94979610 MsG0380017326.01.T01:CDS 40.0%
CGAACATGGTCAATATACGA+CGG + Chr3:94979579-94979598 None:intergenic 40.0%
GCAAGAATCAAATCAACCGT+GGG + Chr3:94979750-94979769 None:intergenic 40.0%
CCAACCGTTCGAAAACTCAA+AGG + Chr3:94979819-94979838 None:intergenic 45.0%
CCAGGTATTTCTCACGAACA+TGG + Chr3:94979593-94979612 None:intergenic 45.0%
CCATGTTCGTGAGAAATACC+TGG - Chr3:94979590-94979609 MsG0380017326.01.T01:CDS 45.0%
TCAACCGTGGGTTTATTAGG+AGG + Chr3:94979738-94979757 None:intergenic 45.0%
!! CCTTTGAGTTTTCGAACGGT+TGG - Chr3:94979816-94979835 MsG0380017326.01.T01:CDS 45.0%
ACCTGGGTCGTTGGATTTGT+GGG - Chr3:94979607-94979626 MsG0380017326.01.T01:CDS 50.0%
GTGAGAAATACCTGGGTCGT+TGG - Chr3:94979598-94979617 MsG0380017326.01.T01:CDS 50.0%
TACCTGGGTCGTTGGATTTG+TGG - Chr3:94979606-94979625 MsG0380017326.01.T01:CDS 50.0%
TTCGGTCTTTGGATTCTCCG+AGG - Chr3:94979787-94979806 MsG0380017326.01.T01:CDS 50.0%
! GATTTGTGGGCTATGTGCAG+AGG - Chr3:94979620-94979639 MsG0380017326.01.T01:CDS 50.0%
! GTGCTACTGTTGCGGCTTAA+CGG - Chr3:94979542-94979561 MsG0380017326.01.T01:CDS 50.0%
CTACTGTTGCGGCTTAACGG+AGG - Chr3:94979545-94979564 MsG0380017326.01.T01:CDS 55.0%
GCCGCAACAGTAGCACTTCA+CGG + Chr3:94979538-94979557 None:intergenic 55.0%
! GCCGTGAAGTGCTACTGTTG+CGG - Chr3:94979534-94979553 MsG0380017326.01.T01:CDS 55.0%
GCCCACAAATCCAACGACCC+AGG + Chr3:94979611-94979630 None:intergenic 60.0%
AACCGAAACCGCGGCGACGA+TGG - Chr3:94979503-94979522 MsG0380017326.01.T01:CDS 65.0%
CTCCATCGTCGCCGCGGTTT+CGG + Chr3:94979508-94979527 None:intergenic 65.0%
TACCACCTCCATCGTCGCCG+CGG + Chr3:94979514-94979533 None:intergenic 65.0%
AACCGCGGCGACGATGGAGG+TGG - Chr3:94979509-94979528 MsG0380017326.01.T01:CDS 70.0%
CGAAACCGCGGCGACGATGG+AGG - Chr3:94979506-94979525 MsG0380017326.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 94979495 94979854 94979495 ID=MsG0380017326.01;Name=MsG0380017326.01
Chr3 mRNA 94979495 94979854 94979495 ID=MsG0380017326.01.T01;Parent=MsG0380017326.01;Name=MsG0380017326.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|119
Chr3 exon 94979495 94979854 94979495 ID=MsG0380017326.01.T01:exon:2150;Parent=MsG0380017326.01.T01
Chr3 CDS 94979495 94979854 94979495 ID=MsG0380017326.01.T01:cds;Parent=MsG0380017326.01.T01
Chr3 mRNA 94979495 94979827 94979495 ID=MsG0380017326.01.T02;Parent=MsG0380017326.01;Name=MsG0380017326.01.T02;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|110
Chr3 exon 94979495 94979827 94979495 ID=MsG0380017326.01.T02:exon:2151;Parent=MsG0380017326.01.T02
Chr3 CDS 94979495 94979827 94979495 ID=MsG0380017326.01.T02:cds;Parent=MsG0380017326.01.T02
Gene Sequence

>MsG0380017326.01.T02

ATGGAGGTGGTAGCCGTGAAGTGCTACTGTTGCGGCTTAACGGAGGAGTGCACTCCGTCGTATATTGACCATGTTCGTGAGAAATACCTGGGTCGTTGGATTTGTGGGCTATGTGCAGAGGCCGTGAAAGAAGAAGCTTTGAAATCGAAAAGAGATATTAGCACCGATGAAGCTCTTAATCAACACATGAATTTCAGAACTTTAACATCAAGTCCTCCTAATAAACCCACGGTTGATTTGATTCTTGCTGTGAAACATCTTCTTTTTCGGTCTTTGGATTCTCCGAGGAAACAACCTTTGAGTTTTCGAACGGTTGGTAGATCGCATAGATAA

>MsG0380017326.01.T01

ATGGCTTTAACCGAAACCGCGGCGACGATGGAGGTGGTAGCCGTGAAGTGCTACTGTTGCGGCTTAACGGAGGAGTGCACTCCGTCGTATATTGACCATGTTCGTGAGAAATACCTGGGTCGTTGGATTTGTGGGCTATGTGCAGAGGCCGTGAAAGAAGAAGCTTTGAAATCGAAAAGAGATATTAGCACCGATGAAGCTCTTAATCAACACATGAATTTCAGAACTTTAACATCAAGTCCTCCTAATAAACCCACGGTTGATTTGATTCTTGCTGTGAAACATCTTCTTTTTCGGTCTTTGGATTCTCCGAGGAAACAACCTTTGAGTTTTCGAACGGTTGGTAGATCGCATAGATAA

Protein sequence

>MsG0380017326.01.T02

MEVVAVKCYCCGLTEECTPSYIDHVREKYLGRWICGLCAEAVKEEALKSKRDISTDEALNQHMNFRTLTSSPPNKPTVDLILAVKHLLFRSLDSPRKQPLSFRTVGRSHR*

>MsG0380017326.01.T01

MALTETAATMEVVAVKCYCCGLTEECTPSYIDHVREKYLGRWICGLCAEAVKEEALKSKRDISTDEALNQHMNFRTLTSSPPNKPTVDLILAVKHLLFRSLDSPRKQPLSFRTVGRSHR*