AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014034.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014034.01.T01 MTR_4g050810 84.892 139 1 1 1 119 46 184 2.19e-74 224
MsG0380014034.01.T01 MTR_7g021700 81.379 145 1 1 1 119 317 461 5.93e-70 221
MsG0380014034.01.T01 MTR_4g006100 84.892 139 1 1 1 119 587 725 8.67e-69 222
MsG0380014034.01.T01 MTR_6g042200 97.778 90 2 0 1 90 587 676 3.43e-51 172
MsG0380014034.01.T01 MTR_4g451555 92.958 71 5 0 15 85 13 83 3.19e-40 130
MsG0380014034.01.T02 MTR_4g050810 75.352 142 1 1 1 108 43 184 4.49e-65 200
MsG0380014034.01.T02 MTR_7g021700 72.297 148 1 1 1 108 314 461 5.75e-61 197
MsG0380014034.01.T02 MTR_4g006100 75.352 142 1 1 1 108 584 725 5.06e-60 197
MsG0380014034.01.T02 MTR_6g042200 98.765 81 1 0 1 81 584 664 2.62e-46 158
MsG0380014034.01.T02 MTR_4g451555 92.188 64 5 0 18 81 13 76 6.39e-35 116
MsG0380014034.01.T03 MTR_4g050810 79.048 105 0 1 1 83 80 184 1.22e-46 152
MsG0380014034.01.T03 MTR_4g006100 79.048 105 0 1 1 83 621 725 9.40e-44 150
MsG0380014034.01.T03 MTR_7g021700 74.775 111 0 1 1 83 351 461 1.35e-43 149
MsG0380014034.01.T03 MTR_4g451555 97.727 44 1 0 1 44 33 76 8.58e-23 84.3
MsG0380014034.01.T03 MTR_6g042200 68.919 74 1 1 1 52 621 694 7.63e-21 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014034.01.T01 ATCG01100 78.667 150 15 2 1 133 211 360 2.27e-72 220
MsG0380014034.01.T04 ATCG01100 96.774 31 1 0 1 31 330 360 2.72e-12 58.2
MsG0380014034.01.T02 ATCG01100 74.510 153 8 1 1 122 208 360 4.37e-69 211
MsG0380014034.01.T03 ATCG01100 76.724 116 8 1 1 97 245 360 5.36e-49 159

Find 23 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATTAAAAGGTTTAAAT+AGG 0.230364 3:+48705499 None:intergenic
CAAATAATGAAGAAATTAAA+AGG 0.264621 3:+48705489 None:intergenic
ACTAATTCTTCTTCTGCTTC+TGG 0.274188 3:+48705578 None:intergenic
AGTTGATGCGCAATCAAATA+TGG 0.304070 3:-48705695 None:intergenic
AATAGATTACCGAGAGAAAT+AGG 0.305666 3:+48705243 None:intergenic
GAATATTCGGGTATTCGATT+TGG 0.315991 3:-48705539 MsG0380014034.01.T01:CDS
GGGTTATCAAACAGAATATT+CGG 0.333132 3:-48705552 MsG0380014034.01.T01:CDS
TTTGAAATCAATAAAACATA+TGG 0.341365 3:-48705404 MsG0380014034.01.T01:intron
ATGGATCAATTATTAAATCT+TGG 0.351344 3:-48705282 MsG0380014034.01.T01:CDS
ATTTGTAACAGTTCTTTACT+TGG 0.416318 3:-48705471 MsG0380014034.01.T01:CDS
TCTGCTTCTGGTAAATCAAA+AGG 0.441932 3:+48705590 None:intergenic
ATCAATTATTAAATCTTGGA+TGG 0.477077 3:-48705278 MsG0380014034.01.T01:CDS
GGTTATCAAACAGAATATTC+GGG 0.492242 3:-48705551 MsG0380014034.01.T01:CDS
GTCTTTGCCTAGGCTAAGAA+TGG 0.495418 3:-48705301 MsG0380014034.01.T01:CDS
TTTGTAACAGTTCTTTACTT+GGG 0.522545 3:-48705470 MsG0380014034.01.T01:CDS
TCGTTTCTATCATAACAAGA+TGG 0.528862 3:-48705323 MsG0380014034.01.T01:CDS
AATTGATCCATTCTTAGCCT+AGG 0.530223 3:+48705294 None:intergenic
AACTCAGAAACGAATATGTA+CGG 0.547502 3:+48705428 None:intergenic
GCAGAAGAAGAATTAGTAGC+GGG 0.560096 3:-48705572 MsG0380014034.01.T01:CDS
TAACAAGATGGTCTTTGCCT+AGG 0.562717 3:-48705311 MsG0380014034.01.T01:CDS
AATTTGTGGCGTCAACCTAT+CGG 0.570074 3:-48705659 MsG0380014034.01.T02:CDS
GTAACAGTTCTTTACTTGGG+CGG 0.607549 3:-48705467 MsG0380014034.01.T01:CDS
AGCAGAAGAAGAATTAGTAG+CGG 0.641363 3:-48705573 MsG0380014034.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAATTAAAAGGTTTAAAT+AGG + Chr3:48705403-48705422 None:intergenic 15.0%
!! CAAATAATGAAGAAATTAAA+AGG + Chr3:48705413-48705432 None:intergenic 15.0%
!!! TTTGAAATCAATAAAACATA+TGG - Chr3:48705495-48705514 MsG0380014034.01.T01:CDS 15.0%
!! ATCAATTATTAAATCTTGGA+TGG - Chr3:48705621-48705640 MsG0380014034.01.T01:CDS 20.0%
!! ATGGATCAATTATTAAATCT+TGG - Chr3:48705617-48705636 MsG0380014034.01.T01:CDS 20.0%
! ATTTGTAACAGTTCTTTACT+TGG - Chr3:48705428-48705447 MsG0380014034.01.T01:CDS 25.0%
! TTTGTAACAGTTCTTTACTT+GGG - Chr3:48705429-48705448 MsG0380014034.01.T01:CDS 25.0%
!! CAATAAAACATATGGAGTTT+TGG - Chr3:48705503-48705522 MsG0380014034.01.T01:CDS 25.0%
!!! TTTCTTTTACCTATTTCTCT+CGG - Chr3:48705647-48705666 MsG0380014034.01.T01:CDS 25.0%
AACTCAGAAACGAATATGTA+CGG + Chr3:48705474-48705493 None:intergenic 30.0%
AATAGATTACCGAGAGAAAT+AGG + Chr3:48705659-48705678 None:intergenic 30.0%
AGAAAAACAATGAAACCGAT+AGG + Chr3:48705258-48705277 None:intergenic 30.0%
GGGTTATCAAACAGAATATT+CGG - Chr3:48705347-48705366 MsG0380014034.01.T01:intron 30.0%
GGTTATCAAACAGAATATTC+GGG - Chr3:48705348-48705367 MsG0380014034.01.T01:intron 30.0%
TCGTTTCTATCATAACAAGA+TGG - Chr3:48705576-48705595 MsG0380014034.01.T01:CDS 30.0%
! CGCAATCAAATATGGTTTTT+GGG - Chr3:48705212-48705231 MsG0380014034.01.T01:CDS 30.0%
! GCAATCAAATATGGTTTTTG+GGG - Chr3:48705213-48705232 MsG0380014034.01.T01:CDS 30.0%
AATTGATCCATTCTTAGCCT+AGG + Chr3:48705608-48705627 None:intergenic 35.0%
ACTAATTCTTCTTCTGCTTC+TGG + Chr3:48705324-48705343 None:intergenic 35.0%
AGCAGAAGAAGAATTAGTAG+CGG - Chr3:48705326-48705345 MsG0380014034.01.T01:CDS 35.0%
GAATATTCGGGTATTCGATT+TGG - Chr3:48705360-48705379 MsG0380014034.01.T01:intron 35.0%
TCTGCTTCTGGTAAATCAAA+AGG + Chr3:48705312-48705331 None:intergenic 35.0%
! GCGCAATCAAATATGGTTTT+TGG - Chr3:48705211-48705230 MsG0380014034.01.T01:CDS 35.0%
!! TCAAATATGGTTTTTGGGGA+TGG - Chr3:48705217-48705236 MsG0380014034.01.T01:CDS 35.0%
AATTTGTGGCGTCAACCTAT+CGG - Chr3:48705240-48705259 MsG0380014034.01.T01:CDS 40.0%
GCAGAAGAAGAATTAGTAGC+GGG - Chr3:48705327-48705346 MsG0380014034.01.T01:intron 40.0%
GTAACAGTTCTTTACTTGGG+CGG - Chr3:48705432-48705451 MsG0380014034.01.T01:CDS 40.0%
TAACAAGATGGTCTTTGCCT+AGG - Chr3:48705588-48705607 MsG0380014034.01.T01:CDS 40.0%
!! GTTTTTGGGGATGGAATTTG+TGG - Chr3:48705226-48705245 MsG0380014034.01.T01:CDS 40.0%
GTCTTTGCCTAGGCTAAGAA+TGG - Chr3:48705598-48705617 MsG0380014034.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48705209 48705712 48705209 ID=MsG0380014034.01;Name=MsG0380014034.01
Chr3 mRNA 48705209 48705669 48705209 ID=MsG0380014034.01.T01;Parent=MsG0380014034.01;Name=MsG0380014034.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|133
Chr3 exon 48705209 48705345 48705209 ID=MsG0380014034.01.T01:exon:57101;Parent=MsG0380014034.01.T01
Chr3 exon 48705405 48705669 48705405 ID=MsG0380014034.01.T01:exon:57100;Parent=MsG0380014034.01.T01
Chr3 CDS 48705405 48705669 48705405 ID=MsG0380014034.01.T01:cds;Parent=MsG0380014034.01.T01
Chr3 CDS 48705209 48705345 48705209 ID=MsG0380014034.01.T01:cds;Parent=MsG0380014034.01.T01
Chr3 mRNA 48705209 48705678 48705209 ID=MsG0380014034.01.T02;Parent=MsG0380014034.01;Name=MsG0380014034.01.T02;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|122
Chr3 exon 48705209 48705333 48705209 ID=MsG0380014034.01.T02:exon:57103;Parent=MsG0380014034.01.T02
Chr3 exon 48705435 48705678 48705435 ID=MsG0380014034.01.T02:exon:57102;Parent=MsG0380014034.01.T02
Chr3 CDS 48705435 48705678 48705435 ID=MsG0380014034.01.T02:cds;Parent=MsG0380014034.01.T02
Chr3 CDS 48705209 48705333 48705209 ID=MsG0380014034.01.T02:cds;Parent=MsG0380014034.01.T02
Chr3 mRNA 48705209 48705567 48705209 ID=MsG0380014034.01.T03;Parent=MsG0380014034.01;Name=MsG0380014034.01.T03;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|97
Chr3 exon 48705209 48705369 48705209 ID=MsG0380014034.01.T03:exon:57105;Parent=MsG0380014034.01.T03
Chr3 exon 48705435 48705567 48705435 ID=MsG0380014034.01.T03:exon:57104;Parent=MsG0380014034.01.T03
Chr3 CDS 48705435 48705567 48705435 ID=MsG0380014034.01.T03:cds;Parent=MsG0380014034.01.T03
Chr3 CDS 48705209 48705369 48705209 ID=MsG0380014034.01.T03:cds;Parent=MsG0380014034.01.T03
Chr3 mRNA 48705209 48705304 48705209 ID=MsG0380014034.01.T04;Parent=MsG0380014034.01;Name=MsG0380014034.01.T04;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|31
Chr3 exon 48705209 48705304 48705209 ID=MsG0380014034.01.T04:exon:57106;Parent=MsG0380014034.01.T04
Chr3 CDS 48705209 48705304 48705209 ID=MsG0380014034.01.T04:cds;Parent=MsG0380014034.01.T04
Chr3 mRNA 48705620 48705712 48705620 ID=MsG0380014034.01.T05;Parent=MsG0380014034.01;Name=MsG0380014034.01.T05;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|30
Chr3 exon 48705620 48705712 48705620 ID=MsG0380014034.01.T05:exon:57107;Parent=MsG0380014034.01.T05
Chr3 CDS 48705620 48705712 48705620 ID=MsG0380014034.01.T05:cds;Parent=MsG0380014034.01.T05
Chr3 mRNA 48705620 48705685 48705620 ID=MsG0380014034.01.T06;Parent=MsG0380014034.01;Name=MsG0380014034.01.T06;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|21
Chr3 exon 48705620 48705685 48705620 ID=MsG0380014034.01.T06:exon:57108;Parent=MsG0380014034.01.T06
Chr3 CDS 48705620 48705685 48705620 ID=MsG0380014034.01.T06:cds;Parent=MsG0380014034.01.T06
Gene Sequence

>MsG0380014034.01.T01

CAACCTATCGGTTTCATTGTTTTTCTAATTTCTTCACTAGCAGAATGTGAAAGATTACCTTTTGATTTACCAGAAGCAGAAGAAGAATTAGTAGCGGGTTATCAAACAGAATATTCGGGTATTCGATTTGGTTTATTTTACGTTGCTTCCTATTTAAACCTTTTAATTTCTTCATTATTTGTAACAGTTCTTTACTTGGGCGGTTCAAATATCTCTATTCCGTACATATTCGTTTCTGAGTTTTTTGAAATCAATAAAACATATGTCGTTTCTATCATAACAAGATGGTCTTTGCCTAGGCTAAGAATGGATCAATTATTAAATCTTGGATGGAAATTTCTTTTACCTATTTCTCTCGGTAATCTATTATTAACAACTTCGTCTCAATTGTTTTCACTGTAA

>MsG0380014034.01.T04

ATGGATCAATTATTAAATCTTGGATGGAAATTTCTTTTACCTATTTCTCTCGGTAATCTATTATTAACAACTTCGTCTCAATTGTTTTCACTGTAA

>MsG0380014034.01.T02

TTGTGGCGTCAACCTATCGGTTTCATTGTTTTTCTAATTTCTTCACTAGCAGAATGTGAAAGATTACCTTTTGATTTACCAGAAGCAGAAGAAGAATTAGTAGCGGGTTATCAAACAGAATATTCGGGTATTCGATTTGGTTTATTTTACGTTGCTTCCTATTTAAACCTTTTAATTTCTTCATTATTTGTAACAGTTCTTTACTTGGGCGGTTCAAATATCTCTATTCCGTACATATTCGTTTTAACAAGATGGTCTTTGCCTAGGCTAAGAATGGATCAATTATTAAATCTTGGATGGAAATTTCTTTTACCTATTTCTCTCGGTAATCTATTATTAACAACTTCGTCTCAATTGTTTTCACTGTAA

>MsG0380014034.01.T03

CAAACAGAATATTCGGGTATTCGATTTGGTTTATTTTACGTTGCTTCCTATTTAAACCTTTTAATTTCTTCATTATTTGTAACAGTTCTTTACTTGGGCGGTTCAAATATCTCTATTCCGTACATATTCGTTTTAGCTAAAACTTATTTTTTCTTATTCGTTTCTATCATAACAAGATGGTCTTTGCCTAGGCTAAGAATGGATCAATTATTAAATCTTGGATGGAAATTTCTTTTACCTATTTCTCTCGGTAATCTATTATTAACAACTTCGTCTCAATTGTTTTCACTGTAA

>MsG0380014034.01.T05

ATGCGCAATCAAATATGGTTTTTGGGGATGGAATTTGTGGCGTCAACCTATCGGTTTCATTGTTTTTCTAATTTCTTCACTAGCAGAATGTGA

>MsG0380014034.01.T06

ATGGAATTTGTGGCGTCAACCTATCGGTTTCATTGTTTTTCTAATTTCTTCACTAGCAGAATGTGA

Protein sequence

>MsG0380014034.01.T01

QPIGFIVFLISSLAECERLPFDLPEAEEELVAGYQTEYSGIRFGLFYVASYLNLLISSLFVTVLYLGGSNISIPYIFVSEFFEINKTYVVSIITRWSLPRLRMDQLLNLGWKFLLPISLGNLLLTTSSQLFSL*

>MsG0380014034.01.T04

MDQLLNLGWKFLLPISLGNLLLTTSSQLFSL*

>MsG0380014034.01.T02

LWRQPIGFIVFLISSLAECERLPFDLPEAEEELVAGYQTEYSGIRFGLFYVASYLNLLISSLFVTVLYLGGSNISIPYIFVLTRWSLPRLRMDQLLNLGWKFLLPISLGNLLLTTSSQLFSL*

>MsG0380014034.01.T03

QTEYSGIRFGLFYVASYLNLLISSLFVTVLYLGGSNISIPYIFVLAKTYFFLFVSIITRWSLPRLRMDQLLNLGWKFLLPISLGNLLLTTSSQLFSL*

>MsG0380014034.01.T05

MRNQIWFLGMEFVASTYRFHCFSNFFTSRM*

>MsG0380014034.01.T06

MEFVASTYRFHCFSNFFTSRM*