AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010137.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010137.01.T01 MTR_7g021710 98.958 96 1 0 1 96 81 176 4.69e-63 192
MsG0280010137.01.T01 MTR_4g006110 98.958 96 1 0 1 96 81 176 4.74e-63 192
MsG0280010137.01.T01 MTR_6g042220 97.917 96 2 0 1 96 81 176 3.21e-62 187
MsG0280010137.01.T01 MTR_5g063410 94.792 96 5 0 1 96 81 176 1.16e-58 181
MsG0280010137.01.T01 MTR_0002s0160 68.750 96 30 0 1 96 188 283 2.77e-42 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010137.01.T01 ATCG01080 78.125 96 21 0 1 96 81 176 1.62e-48 152

Find 10 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATGGTACGGAATTATT+TGG 0.128600 2:+65287440 MsG0280010137.01.T01:CDS
ATTAAAATCTTGGTAATATT+CGG 0.281186 2:-65287338 None:intergenic
TCTTGCTCAATAATCTGATT+TGG 0.326155 2:-65287472 None:intergenic
ATAAGTACTAGTCAACAAAT+TGG 0.328309 2:+65287499 MsG0280010137.01.T01:CDS
ATTCCGTACCATGAAGTATC+TGG 0.340621 2:-65287433 None:intergenic
CTATTATTCCAGATACTTCA+TGG 0.342688 2:+65287425 MsG0280010137.01.T01:CDS
ATTATTGAAATTAGTTCAAA+TGG 0.371871 2:-65287550 None:intergenic
CAGTCCAAAGATTAAAATCT+TGG 0.418502 2:-65287348 None:intergenic
AATCTTTGGACTGTTGGGGA+TGG 0.537956 2:+65287358 MsG0280010137.01.T01:CDS
ATTCCAGATACTTCATGGTA+CGG 0.544710 2:+65287430 MsG0280010137.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTGAAATTAGTTCAAA+TGG - Chr2:65287553-65287572 None:intergenic 15.0%
!!! ATTAAAATCTTGGTAATATT+CGG - Chr2:65287341-65287360 None:intergenic 15.0%
!! TTCAAATGGAAGAAAAAAAT+CGG - Chr2:65287539-65287558 None:intergenic 20.0%
!!! ATTACCAAGATTTTAATCTT+TGG + Chr2:65287344-65287363 MsG0280010137.01.T01:CDS 20.0%
! ATAAGTACTAGTCAACAAAT+TGG + Chr2:65287499-65287518 MsG0280010137.01.T01:CDS 25.0%
!!! ATTCTTTTAGTTGCTTTGAT+AGG + Chr2:65287571-65287590 MsG0280010137.01.T01:CDS 25.0%
!!! ATTTTAATCTTTGGACTGTT+GGG + Chr2:65287353-65287372 MsG0280010137.01.T01:CDS 25.0%
CAGTCCAAAGATTAAAATCT+TGG - Chr2:65287351-65287370 None:intergenic 30.0%
CTATTATTCCAGATACTTCA+TGG + Chr2:65287425-65287444 MsG0280010137.01.T01:CDS 30.0%
TCTTGCTCAATAATCTGATT+TGG - Chr2:65287475-65287494 None:intergenic 30.0%
! TTTTAATCTTTGGACTGTTG+GGG + Chr2:65287354-65287373 MsG0280010137.01.T01:CDS 30.0%
!! GATTTTAATCTTTGGACTGT+TGG + Chr2:65287352-65287371 MsG0280010137.01.T01:CDS 30.0%
ATTCCAGATACTTCATGGTA+CGG + Chr2:65287430-65287449 MsG0280010137.01.T01:CDS 35.0%
CTTCATGGTACGGAATTATT+TGG + Chr2:65287440-65287459 MsG0280010137.01.T01:CDS 35.0%
! ATTCCGTACCATGAAGTATC+TGG - Chr2:65287436-65287455 None:intergenic 40.0%
AATCTTTGGACTGTTGGGGA+TGG + Chr2:65287358-65287377 MsG0280010137.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr2 gene 65287328 65287618 65287328 ID=MsG0280010137.01;Name=MsG0280010137.01
Chr2 mRNA 65287328 65287618 65287328 ID=MsG0280010137.01.T01;Parent=MsG0280010137.01;Name=MsG0280010137.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|96
Chr2 exon 65287328 65287618 65287328 ID=MsG0280010137.01.T01:exon:17743;Parent=MsG0280010137.01.T01
Chr2 CDS 65287328 65287618 65287328 ID=MsG0280010137.01.T01:cds;Parent=MsG0280010137.01.T01
Chr2 mRNA 65287444 65287488 65287444 ID=MsG0280010137.01.T02;Parent=MsG0280010137.01;Name=MsG0280010137.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|14
Chr2 exon 65287444 65287488 65287444 ID=MsG0280010137.01.T02:exon:17744;Parent=MsG0280010137.01.T02
Chr2 CDS 65287444 65287488 65287444 ID=MsG0280010137.01.T02:cds;Parent=MsG0280010137.01.T02
Gene Sequence

>MsG0280010137.01.T02

ATGGTACGGAATTATTTGGACTACAAGACCAAATCAGATTATTGA

>MsG0280010137.01.T01

ATGAATAGTTCCGAATATTACCAAGATTTTAATCTTTGGACTGTTGGGGATGGAATTACTTTGATAGTTTGTACAAGTATTTTTGTTTCACTAATAACTATTATTCCAGATACTTCATGGTACGGAATTATTTGGACTACAAGACCAAATCAGATTATTGAGCAAGATTTGATAAGTACTAGTCAACAAATTGGAATTCATTTATCAACCGATTTTTTTCTTCCATTTGAACTAATTTCAATAATTCTTTTAGTTGCTTTGATAGGTGCAATTGTCGTAGCTCGCCAGTAA

Protein sequence

>MsG0280010137.01.T02

MVRNYLDYKTKSDY*

>MsG0280010137.01.T01

MNSSEYYQDFNLWTVGDGITLIVCTSIFVSLITIIPDTSWYGIIWTTRPNQIIEQDLISTSQQIGIHLSTDFFLPFELISIILLVALIGAIVVARQ*