AlfalfaGEDB | MsG0380017155.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017155.01

KEGG: K13126
GO: GO:0003723
PFAM: PF00658

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017155.01.T01	AFK34295.1	100	80	0	0	1	80	28	107	1.33E-48	159

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017155.01.T01	Q9FXA2	75.641	78	19	0	1	78	592	669	8.90E-31	115

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017155.01.T01	G7J6Y1	100.000	80	0	0	1	80	543	622	2.79e-44	160

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000096.01	MsG0380017155.01	0.857529	1.534112e-62	3.797974e-59
MsG0180000719.01	MsG0380017155.01	-0.802949	4.400407e-49	2.183720e-46
MsG0180001588.01	MsG0380017155.01	0.811617	6.399629e-51	3.980006e-48
MsG0180003258.01	MsG0380017155.01	0.801503	8.730812e-49	4.176427e-46
MsG0180004064.01	MsG0380017155.01	0.817764	2.784013e-52	2.045728e-49
MsG0180004609.01	MsG0380017155.01	0.816369	5.731160e-52	4.053420e-49
MsG0180004625.01	MsG0380017155.01	0.811099	8.292549e-51	5.087466e-48
MsG0180005384.01	MsG0380017155.01	0.844252	8.483357e-59	1.361491e-55
MsG0180005767.01	MsG0380017155.01	-0.810307	1.230250e-50	7.389881e-48
MsG0280006301.01	MsG0380017155.01	0.823789	1.146153e-53	9.968178e-51
MsG0380015857.01	MsG0380017155.01	0.843466	1.377377e-58	2.156914e-55
MsG0380016089.01	MsG0380017155.01	-0.801211	1.002053e-48	4.758188e-46
MsG0380016528.01	MsG0380017155.01	-0.828480	8.784579e-55	8.742264e-52
MsG0380016760.01	MsG0380017155.01	0.838269	3.178493e-57	4.234962e-54
MsG0380017155.01	MsG0380017156.01	0.826746	2.291120e-54	2.167552e-51
MsG0380017155.01	MsG0480020911.01	0.804555	2.042211e-49	1.056125e-46
MsG0380017155.01	MsG0480022006.01	0.840404	8.874322e-58	1.262815e-54
MsG0380017155.01	MsG0480022789.01	0.802236	6.173170e-49	3.008404e-46
MsG0380017155.01	MsG0480023380.01	0.807078	6.026182e-50	3.325031e-47
MsG0380017155.01	MsG0480023774.01	0.825244	5.209934e-54	4.722759e-51
MsG0380017155.01	MsG0580029703.01	0.811020	8.627446e-51	5.282252e-48
MsG0380017155.01	MsG0780038383.01	0.807859	4.115116e-50	2.317186e-47
MsG0380017155.01	MsG0780041522.01	0.821064	4.924699e-53	3.965449e-50
MsG0380017155.01	MsG0780041790.01	0.806930	6.474690e-50	3.559021e-47
MsG0380017155.01	MsG0880045836.01	0.811169	8.008044e-51	4.921868e-48
MsG0380017155.01	MsG0880047090.01	0.807801	4.233496e-50	2.380361e-47
MsG0380017155.01	MsG0880047310.01	0.829968	3.824295e-55	3.974897e-52
MsG0280006717.01	MsG0380017155.01	0.801953	7.059664e-49	3.415577e-46
MsG0280007055.01	MsG0380017155.01	0.804053	2.598439e-49	1.326618e-46
MsG0280007084.01	MsG0380017155.01	0.809235	2.091614e-50	1.221465e-47
MsG0280010519.01	MsG0380017155.01	0.824915	6.229956e-54	5.595005e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017155.01.T01	MTR_3g102040	100.000	80	0	0	1	80	543	622	7.09e-48	160
MsG0380017155.01.T01	MTR_1g019150	81.818	77	14	0	1	77	571	647	4.94e-37	130
MsG0380017155.01.T01	MTR_4g085540	77.941	68	15	0	1	68	575	642	9.12e-28	104
MsG0380017155.01.T01	MTR_1g097440	61.538	78	23	3	1	75	572	645	5.27e-20	82.4
MsG0380017155.01.T01	MTR_0989s0020	59.701	67	26	1	1	66	15	81	1.78e-19	75.1
MsG0380017155.01.T01	MTR_0913s0010	61.667	60	22	1	1	59	1	60	2.21e-15	64.3
MsG0380017155.01.T01	MTR_7g102730	64.286	56	20	0	1	56	551	606	1.88e-14	66.6
MsG0380017155.01.T01	MTR_6g040190	61.667	60	22	1	1	59	553	612	4.34e-14	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017155.01.T01	AT1G49760	75.641	78	19	0	1	78	592	669	9.08e-32	115
MsG0380017155.01.T01	AT1G49760	75.641	78	19	0	1	78	592	669	9.08e-32	115
MsG0380017155.01.T01	AT2G23350	66.279	86	21	1	1	78	577	662	3.35e-28	105
MsG0380017155.01.T01	AT4G34110	73.333	75	17	1	1	75	558	629	2.35e-27	103
MsG0380017155.01.T01	AT1G71770	65.753	73	25	0	1	73	607	679	1.90e-22	89.7
MsG0380017155.01.T01	AT1G71770	65.753	73	25	0	1	73	607	679	1.90e-22	89.7
MsG0380017155.01.T01	AT1G22760	63.158	76	23	1	1	76	590	660	3.10e-21	86.3
MsG0380017155.01.T01	AT3G19350	47.541	61	32	0	1	61	34	94	5.35e-13	59.3
MsG0380017155.01.T01	AT2G36660	57.143	56	24	0	1	56	528	583	1.22e-11	58.9

Find 25 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACTACTTGGTTACTGGATT+TGG	0.244519	3:-92708991	MsG0380017155.01.T01:three_prime_UTR
GTCTGTTTGACTTTGTTTCT+AGG	0.281094	3:-92708969	MsG0380017155.01.T01:three_prime_UTR
GCTGGAGCTGCAAAGGTTAC+TGG	0.301054	3:-92709371	MsG0380017155.01.T01:CDS
TCCAGTAACCAAGTAGTAAA+AGG	0.307038	3:+92708996	None:intergenic
AAAGGTTACTGGGATGCTTT+TGG	0.320904	3:-92709360	MsG0380017155.01.T01:CDS
GACTAAAGTTGCTGAGGCCG+TGG	0.464712	3:-92709285	MsG0380017155.01.T01:CDS
ACTTTAGTCTTGAGAGCCTC+TGG	0.470970	3:+92709299	None:intergenic
AGAGAAGCTAGTTGATCAGT+TGG	0.477475	3:+92709224	None:intergenic
ATCTCGGTAAACTGTCATGC+AGG	0.479765	3:+92709171	None:intergenic
TTCAAAGAACGCATGTATCT+CGG	0.489583	3:+92709155	None:intergenic
CTGGAGCTGCAAAGGTTACT+GGG	0.499264	3:-92709370	MsG0380017155.01.T01:CDS
GAGAAGCTAGTTGATCAGTT+GGG	0.512991	3:+92709225	None:intergenic
TCAATCAGATGCAACACCTC+AGG	0.532271	3:+92709326	None:intergenic
GTAGAGAAGATTGAGCATGC+TGG	0.539286	3:-92709389	MsG0380017155.01.T01:CDS
TGCTCAATCTTCTCTACTAG+AGG	0.548946	3:+92709395	None:intergenic
TGAGCATGCTGGAGCTGCAA+AGG	0.560400	3:-92709378	MsG0380017155.01.T01:CDS
TGAATGGATTCATAGATGCT+TGG	0.574263	3:-92709431	None:intergenic
TCAGATGCAACACCTCAGGC+TGG	0.586095	3:+92709330	None:intergenic
GCAATATGCTAGCTAATCCA+AGG	0.589078	3:+92709040	None:intergenic
TTTGGAGATGGACCAGCCTG+AGG	0.621857	3:-92709342	MsG0380017155.01.T01:CDS
GCTCAATCTTCTCTACTAGA+GGG	0.623607	3:+92709396	None:intergenic
AACATTTCTCAACACATCCA+CGG	0.633247	3:+92709268	None:intergenic
TCTCAAGACTAAAGTTGCTG+AGG	0.646425	3:-92709291	MsG0380017155.01.T01:CDS
TCTCGGTAAACTGTCATGCA+GGG	0.659144	3:+92709172	None:intergenic
GCATCTGATTGAGTCACCAG+AGG	0.683305	3:-92709315	MsG0380017155.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACATTTCTCAACACATCCA+CGG	+	Chr3:92709090-92709109	None:intergenic	35.0%
	TACTACTTGGTTACTGGATT+TGG	-	Chr3:92709364-92709383	MsG0380017155.01.T01:CDS	35.0%
	TCCAGTAACCAAGTAGTAAA+AGG	+	Chr3:92709362-92709381	None:intergenic	35.0%
	TTCAAAGAACGCATGTATCT+CGG	+	Chr3:92709203-92709222	None:intergenic	35.0%
!	CTTCGTTTCCTTTTACTACT+TGG	-	Chr3:92709351-92709370	MsG0380017155.01.T01:CDS	35.0%
!	GTCTGTTTGACTTTGTTTCT+AGG	-	Chr3:92709386-92709405	MsG0380017155.01.T01:CDS	35.0%
!	TCCTTTTACTACTTGGTTAC+TGG	-	Chr3:92709358-92709377	MsG0380017155.01.T01:CDS	35.0%
	AGAGAAGCTAGTTGATCAGT+TGG	+	Chr3:92709134-92709153	None:intergenic	40.0%
	GAGAAGCTAGTTGATCAGTT+GGG	+	Chr3:92709133-92709152	None:intergenic	40.0%
	GCAATATGCTAGCTAATCCA+AGG	+	Chr3:92709318-92709337	None:intergenic	40.0%
	GCTCAATCTTCTCTACTAGA+GGG	+	Chr3:92708962-92708981	None:intergenic	40.0%
	TCTCAAGACTAAAGTTGCTG+AGG	-	Chr3:92709064-92709083	MsG0380017155.01.T01:three_prime_UTR	40.0%
	TGCTCAATCTTCTCTACTAG+AGG	+	Chr3:92708963-92708982	None:intergenic	40.0%
!	AAAGGTTACTGGGATGCTTT+TGG	-	Chr3:92708995-92709014	MsG0380017155.01.T01:three_prime_UTR	40.0%
!!	ACGTAACAGTTTTGTGCTTC+TGG	-	Chr3:92709268-92709287	MsG0380017155.01.T01:CDS	40.0%
!!	GTAGGATTAGCGATTTTCCT+TGG	-	Chr3:92709298-92709317	MsG0380017155.01.T01:CDS	40.0%
!!!	GTTTTGTGCTTCTGGCTTTT+TGG	-	Chr3:92709276-92709295	MsG0380017155.01.T01:CDS	40.0%
	ATCTCGGTAAACTGTCATGC+AGG	+	Chr3:92709187-92709206	None:intergenic	45.0%
	GTAGAGAAGATTGAGCATGC+TGG	-	Chr3:92708966-92708985	MsG0380017155.01.T01:three_prime_UTR	45.0%
	TCAATCAGATGCAACACCTC+AGG	+	Chr3:92709032-92709051	None:intergenic	45.0%
	TCTCGGTAAACTGTCATGCA+GGG	+	Chr3:92709186-92709205	None:intergenic	45.0%
!!	ACGTTACAGTTTTGTGCGTC+TGG	-	Chr3:92709222-92709241	MsG0380017155.01.T01:CDS	45.0%
!!	ACTTTAGTCTTGAGAGCCTC+TGG	+	Chr3:92709059-92709078	None:intergenic	45.0%
!!	TACTGGGATGCTTTTGGAGA+TGG	-	Chr3:92709001-92709020	MsG0380017155.01.T01:three_prime_UTR	45.0%
!!!	GTTTTGTGCGTCTGGCTTTT+TGG	-	Chr3:92709230-92709249	MsG0380017155.01.T01:CDS	45.0%
!!!	TGTGCTTCTGGCTTTTTGGT+AGG	-	Chr3:92709280-92709299	MsG0380017155.01.T01:CDS	45.0%
	CTGGAGCTGCAAAGGTTACT+GGG	-	Chr3:92708985-92709004	MsG0380017155.01.T01:three_prime_UTR	50.0%
	GCATCTGATTGAGTCACCAG+AGG	-	Chr3:92709040-92709059	MsG0380017155.01.T01:intron	50.0%
!!!	TGTGCGTCTGGCTTTTTGGT+AGG	-	Chr3:92709234-92709253	MsG0380017155.01.T01:CDS	50.0%
	GACTAAAGTTGCTGAGGCCG+TGG	-	Chr3:92709070-92709089	MsG0380017155.01.T01:three_prime_UTR	55.0%
	GCTGGAGCTGCAAAGGTTAC+TGG	-	Chr3:92708984-92709003	MsG0380017155.01.T01:three_prime_UTR	55.0%
	TCAGATGCAACACCTCAGGC+TGG	+	Chr3:92709028-92709047	None:intergenic	55.0%
	TGAGCATGCTGGAGCTGCAA+AGG	-	Chr3:92708977-92708996	MsG0380017155.01.T01:three_prime_UTR	55.0%
	TTTGGAGATGGACCAGCCTG+AGG	-	Chr3:92709013-92709032	MsG0380017155.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	92708939	92709438	92708939	ID=MsG0380017155.01;Name=MsG0380017155.01
Chr3	mRNA	92708939	92709438	92708939	ID=MsG0380017155.01.T01;Parent=MsG0380017155.01;Name=MsG0380017155.01.T01;_AED=0.42;_eAED=0.42;_QI=0\|1\|1\|1\|0.22\|0.3\|10\|236\|80
Chr3	exon	92708939	92709030	92708939	ID=MsG0380017155.01.T01:exon:15723;Parent=MsG0380017155.01.T01
Chr3	exon	92709052	92709438	92709052	ID=MsG0380017155.01.T01:exon:15722;Parent=MsG0380017155.01.T01
Chr3	CDS	92709196	92709438	92709196	ID=MsG0380017155.01.T01:cds;Parent=MsG0380017155.01.T01
Chr3	three_prime_UTR	92709052	92709195	92709052	ID=MsG0380017155.01.T01:three_prime_utr;Parent=MsG0380017155.01.T01
Chr3	three_prime_UTR	92708939	92709030	92708939	ID=MsG0380017155.01.T01:three_prime_utr;Parent=MsG0380017155.01.T01

Gene Sequence	>MsG0380017155.01.T01 ATGCTTGGTGAAGTTTTATACCCTCTAGTAGAGAAGATTGAGCATGCTGGAGCTGCAAAGGTTACTGGGATGCTTTTGGAGATGGACCAGCCTGAGGTGTTGCATCTGATTGAGTCACCAGAGGCTCTCAAGACTAAAGTTGCTGAGGCCGTGGATGTGTTGAGAAATGTTGCACAGCAGCAATCAAACAGCCCAACTGATCAACTAGCTTCTCTCTCTCTTAACGACAATCTTGAGTCTTAG
Protein sequence	>MsG0380017155.01.T01 MLGEVLYPLVEKIEHAGAAKVTGMLLEMDQPEVLHLIESPEALKTKVAEAVDVLRNVAQQQSNSPTDQLASLSLNDNLES*