Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041790.01.T01 | XP_024625385.1 | 96.491 | 114 | 3 | 1 | 1 | 114 | 1 | 113 | 2.32E-67 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041790.01.T01 | O23095 | 56.25 | 112 | 48 | 1 | 2 | 113 | 3 | 113 | 7.26E-27 | 98.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041790.01.T01 | G7L532 | 96.491 | 114 | 3 | 1 | 1 | 114 | 1 | 113 | 1.11e-67 | 208 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048040.01 | MsG0780041790.01 | 0.804086 | 2.556773e-49 | 1.306432e-46 |
| MsG0080048922.01 | MsG0780041790.01 | 0.835186 | 1.942605e-56 | 2.357643e-53 |
| MsG0180000096.01 | MsG0780041790.01 | 0.871397 | 7.051600e-67 | 2.840358e-63 |
| MsG0180000118.01 | MsG0780041790.01 | 0.875568 | 2.780719e-68 | 1.304765e-64 |
| MsG0180001169.01 | MsG0780041790.01 | 0.813634 | 2.317269e-51 | 1.521343e-48 |
| MsG0180001829.01 | MsG0780041790.01 | 0.830192 | 3.371772e-55 | 3.527517e-52 |
| MsG0180003585.01 | MsG0780041790.01 | 0.863190 | 2.970377e-64 | 8.927882e-61 |
| MsG0180003897.01 | MsG0780041790.01 | 0.860753 | 1.657666e-63 | 4.580593e-60 |
| MsG0180004625.01 | MsG0780041790.01 | 0.806835 | 6.783537e-50 | 3.719615e-47 |
| MsG0180004829.01 | MsG0780041790.01 | 0.822595 | 2.177414e-53 | 1.830879e-50 |
| MsG0180005384.01 | MsG0780041790.01 | 0.911341 | 6.789732e-83 | 1.447972e-78 |
| MsG0180005573.01 | MsG0780041790.01 | 0.810875 | 9.273581e-51 | 5.655831e-48 |
| MsG0180005789.01 | MsG0780041790.01 | 0.834320 | 3.209307e-56 | 3.795454e-53 |
| MsG0280006301.01 | MsG0780041790.01 | 0.849423 | 3.264651e-60 | 6.185393e-57 |
| MsG0780041461.01 | MsG0780041790.01 | 0.832200 | 1.082632e-55 | 1.201523e-52 |
| MsG0780041522.01 | MsG0780041790.01 | 0.871028 | 9.335527e-67 | 3.709653e-63 |
| MsG0780041790.01 | MsG0880041941.01 | 0.833213 | 6.069301e-56 | 6.942835e-53 |
| MsG0780041790.01 | MsG0880042517.01 | 0.844510 | 7.230418e-59 | 1.169952e-55 |
| MsG0780041790.01 | MsG0880042896.01 | 0.840880 | 6.659722e-58 | 9.617673e-55 |
| MsG0780041790.01 | MsG0880043076.01 | 0.869276 | 3.495743e-66 | 1.304130e-62 |
| MsG0780041790.01 | MsG0880044204.01 | 0.816040 | 6.788940e-52 | 4.758594e-49 |
| MsG0780041790.01 | MsG0880045836.01 | 0.813876 | 2.050458e-51 | 1.355058e-48 |
| MsG0780041790.01 | MsG0880046220.01 | 0.850698 | 1.435405e-60 | 2.835554e-57 |
| MsG0780041790.01 | MsG0880046486.01 | 0.828934 | 6.820005e-55 | 6.876890e-52 |
| MsG0780041790.01 | MsG0880047310.01 | 0.836967 | 6.859396e-57 | 8.783289e-54 |
| MsG0380014507.01 | MsG0780041790.01 | 0.846965 | 1.559308e-59 | 2.729793e-56 |
| MsG0680031368.01 | MsG0780041790.01 | 0.857594 | 1.467118e-62 | 3.640525e-59 |
| MsG0680032459.01 | MsG0780041790.01 | 0.847238 | 1.312292e-59 | 2.317582e-56 |
| MsG0680035611.01 | MsG0780041790.01 | 0.835138 | 1.997375e-56 | 2.420447e-53 |
| MsG0480019165.01 | MsG0780041790.01 | 0.839093 | 1.947118e-57 | 2.660609e-54 |
| MsG0480019632.01 | MsG0780041790.01 | 0.812929 | 3.310249e-51 | 2.132034e-48 |
| MsG0480020796.01 | MsG0780041790.01 | 0.873125 | 1.873504e-67 | 8.039208e-64 |
| MsG0480020895.01 | MsG0780041790.01 | 0.827623 | 1.412300e-54 | 1.370617e-51 |
| MsG0480020911.01 | MsG0780041790.01 | 0.883141 | 5.765374e-71 | 3.611440e-67 |
| MsG0480021279.01 | MsG0780041790.01 | 0.879523 | 1.162662e-69 | 6.322367e-66 |
| MsG0480021288.01 | MsG0780041790.01 | 0.841092 | 5.860185e-58 | 8.517352e-55 |
| MsG0480021417.01 | MsG0780041790.01 | 0.862598 | 4.523713e-64 | 1.332183e-60 |
| MsG0480022006.01 | MsG0780041790.01 | 0.896725 | 2.800966e-76 | 3.067982e-72 |
| MsG0480022041.01 | MsG0780041790.01 | 0.800511 | 1.392790e-48 | 6.497101e-46 |
| MsG0480022363.01 | MsG0780041790.01 | 0.851762 | 7.185250e-61 | 1.469437e-57 |
| MsG0480022789.01 | MsG0780041790.01 | 0.823288 | 1.501428e-53 | 1.287276e-50 |
| MsG0480022955.01 | MsG0780041790.01 | 0.817396 | 3.369531e-52 | 2.451105e-49 |
| MsG0480023380.01 | MsG0780041790.01 | 0.852888 | 3.434843e-61 | 7.284624e-58 |
| MsG0480023774.01 | MsG0780041790.01 | 0.901334 | 2.975888e-78 | 4.001999e-74 |
| MsG0480023931.01 | MsG0780041790.01 | 0.857726 | 1.341443e-62 | 3.342998e-59 |
| MsG0480023941.01 | MsG0780041790.01 | 0.831854 | 1.318453e-55 | 1.448659e-52 |
| MsG0480023996.01 | MsG0780041790.01 | 0.830560 | 2.741341e-55 | 2.898440e-52 |
| MsG0580024059.01 | MsG0780041790.01 | 0.849555 | 2.998916e-60 | 5.706233e-57 |
| MsG0580024481.01 | MsG0780041790.01 | 0.831496 | 1.615268e-55 | 1.755856e-52 |
| MsG0580024540.01 | MsG0780041790.01 | 0.894071 | 3.480617e-75 | 3.407868e-71 |
| MsG0580024738.01 | MsG0780041790.01 | 0.874219 | 8.010853e-68 | 3.576446e-64 |
| MsG0580024894.01 | MsG0780041790.01 | 0.853303 | 2.611630e-61 | 5.617116e-58 |
| MsG0580025750.01 | MsG0780041790.01 | 0.844308 | 8.192968e-59 | 1.317262e-55 |
| MsG0380015446.01 | MsG0780041790.01 | 0.813267 | 2.791383e-51 | 1.814329e-48 |
| MsG0380015857.01 | MsG0780041790.01 | 0.901152 | 3.578067e-78 | 4.772072e-74 |
| MsG0380016678.01 | MsG0780041790.01 | 0.842279 | 2.848498e-58 | 4.297394e-55 |
| MsG0380016744.01 | MsG0780041790.01 | 0.840853 | 6.772107e-58 | 9.771526e-55 |
| MsG0380016760.01 | MsG0780041790.01 | 0.893272 | 7.332682e-75 | 6.940423e-71 |
| MsG0380017155.01 | MsG0780041790.01 | 0.806930 | 6.474690e-50 | 3.559021e-47 |
| MsG0380017516.01 | MsG0780041790.01 | 0.835919 | 1.267528e-56 | 1.572492e-53 |
| MsG0380018056.01 | MsG0780041790.01 | 0.803869 | 2.837837e-49 | 1.442027e-46 |
| MsG0480018164.01 | MsG0780041790.01 | 0.879825 | 9.079993e-70 | 4.997068e-66 |
| MsG0580026749.01 | MsG0780041790.01 | 0.809607 | 1.740901e-50 | 1.026785e-47 |
| MsG0280006629.01 | MsG0780041790.01 | 0.851445 | 8.837188e-61 | 1.789100e-57 |
| MsG0280006747.01 | MsG0780041790.01 | 0.867875 | 9.908343e-66 | 3.512852e-62 |
| MsG0280006760.01 | MsG0780041790.01 | 0.809420 | 1.908851e-50 | 1.120252e-47 |
| MsG0280007055.01 | MsG0780041790.01 | 0.899249 | 2.388164e-77 | 2.928515e-73 |
| MsG0280007084.01 | MsG0780041790.01 | 0.821111 | 4.804464e-53 | 3.873360e-50 |
| MsG0280007606.01 | MsG0780041790.01 | 0.861321 | 1.113619e-63 | 3.138838e-60 |
| MsG0280007995.01 | MsG0780041790.01 | 0.851239 | 1.010030e-60 | 2.031274e-57 |
| MsG0280010519.01 | MsG0780041790.01 | 0.835609 | 1.518607e-56 | 1.866510e-53 |
| MsG0780037290.01 | MsG0780041790.01 | 0.802042 | 6.767138e-49 | 3.281564e-46 |
| MsG0780038383.01 | MsG0780041790.01 | 0.869715 | 2.514525e-66 | 9.529839e-63 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041790.01.T01 | MTR_7g118060 | 96.491 | 114 | 3 | 1 | 1 | 114 | 1 | 113 | 2.81e-71 | 208 |
| MsG0780041790.01.T01 | MTR_8g024350 | 56.637 | 113 | 44 | 1 | 1 | 113 | 1 | 108 | 4.43e-32 | 109 |
| MsG0780041790.01.T01 | MTR_3g092600 | 55.752 | 113 | 47 | 1 | 1 | 113 | 1 | 110 | 5.55e-22 | 83.6 |
| MsG0780041790.01.T01 | MTR_2g066750 | 54.867 | 113 | 48 | 1 | 1 | 113 | 1 | 110 | 5.55e-22 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041790.01.T01 | AT5G24510 | 50.435 | 115 | 51 | 2 | 1 | 113 | 1 | 111 | 6.46e-31 | 106 |
| MsG0780041790.01.T01 | AT4G00810 | 56.250 | 112 | 48 | 1 | 2 | 113 | 3 | 113 | 7.40e-28 | 98.6 |
| MsG0780041790.01.T01 | AT4G00810 | 56.250 | 112 | 48 | 1 | 2 | 113 | 3 | 113 | 7.40e-28 | 98.6 |
| MsG0780041790.01.T01 | AT5G47700 | 61.607 | 112 | 42 | 1 | 2 | 113 | 3 | 113 | 5.63e-27 | 96.7 |
| MsG0780041790.01.T01 | AT5G47700 | 61.607 | 112 | 42 | 1 | 2 | 113 | 3 | 113 | 5.63e-27 | 96.7 |
| MsG0780041790.01.T01 | AT1G01100 | 52.679 | 112 | 51 | 1 | 2 | 113 | 3 | 112 | 6.95e-26 | 93.6 |
| MsG0780041790.01.T01 | AT1G01100 | 52.679 | 112 | 51 | 1 | 2 | 113 | 3 | 112 | 6.95e-26 | 93.6 |
| MsG0780041790.01.T01 | AT1G01100 | 52.679 | 112 | 51 | 1 | 2 | 113 | 3 | 112 | 6.95e-26 | 93.6 |
| MsG0780041790.01.T01 | AT1G01100 | 47.321 | 112 | 41 | 1 | 2 | 113 | 3 | 96 | 1.15e-25 | 92.8 |
Find 34 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGAGAGTGACGAGGAAA+TGG | 0.262947 | 7:-95180619 | MsG0780041790.01.T01:CDS |
| CTCTCTTCTTCCTCTGGCTC+TGG | 0.293967 | 7:+95180633 | None:intergenic |
| AGCAGCAGCTGGAGCAGCAG+CGG | 0.297502 | 7:+95180668 | None:intergenic |
| TTCTTCTCAGCAGCAGCAGC+TGG | 0.337075 | 7:+95180657 | None:intergenic |
| ATTCGGCTTGTTCGATGAAT+AGG | 0.347729 | 7:-95180595 | None:intergenic |
| AGCAGCCGCAACAGCTGCTC+CGG | 0.350734 | 7:+95180716 | None:intergenic |
| AGGTTGATGTTGAATCCTTC+TGG | 0.358591 | 7:-95180815 | MsG0780041790.01.T01:CDS |
| CCACCACCTGATGCTGCAAC+TGG | 0.368389 | 7:+95180693 | None:intergenic |
| GCTGCTCCAGTTGCAGCATC+AGG | 0.384530 | 7:-95180699 | MsG0780041790.01.T01:CDS |
| AGCACCACCACCAGCAGCAC+TGG | 0.406072 | 7:+95180740 | None:intergenic |
| TCGTCACTCTCTTCTTCCTC+TGG | 0.421710 | 7:+95180627 | None:intergenic |
| GCTGCTGGTGGTGGTGCTCC+CGG | 0.428131 | 7:-95180735 | MsG0780041790.01.T01:CDS |
| GAAGAGAGTGACGAGGAAAT+GGG | 0.432201 | 7:-95180618 | MsG0780041790.01.T01:CDS |
| CAACAAAGTTGTGATGTTGT+CGG | 0.447259 | 7:+95180848 | None:intergenic |
| GCAGCCGCAACAGCTGCTCC+GGG | 0.457375 | 7:+95180717 | None:intergenic |
| GATTTGATTGCCAGTGCTGC+TGG | 0.480049 | 7:-95180750 | MsG0780041790.01.T01:CDS |
| GTGATGTTGTCGGCAGTTGC+TGG | 0.481555 | 7:+95180858 | None:intergenic |
| ATTGCCAGTGCTGCTGGTGG+TGG | 0.488565 | 7:-95180744 | MsG0780041790.01.T01:CDS |
| CCAGTTGCAGCATCAGGTGG+TGG | 0.493184 | 7:-95180693 | MsG0780041790.01.T01:CDS |
| GGATTCAACATCAACCTTTG+CGG | 0.496398 | 7:+95180821 | None:intergenic |
| CTGGAATGTTGTCATCTTCG+AGG | 0.500813 | 7:+95180877 | None:intergenic |
| AGTGACGAGGAAATGGGATT+CGG | 0.504376 | 7:-95180612 | MsG0780041790.01.T01:CDS |
| GCAAGTTTAGCGAATAAAGC+GGG | 0.558481 | 7:+95180792 | None:intergenic |
| TGTCATCTTCGAGGATGAGA+AGG | 0.559827 | 7:+95180886 | None:intergenic |
| GCTCCAGTTGCAGCATCAGG+TGG | 0.561855 | 7:-95180696 | MsG0780041790.01.T01:CDS |
| TGCTCCCGGAGCAGCTGTTG+CGG | 0.566157 | 7:-95180721 | MsG0780041790.01.T01:CDS |
| AACTTTGTTGAAATCCGCAA+AGG | 0.566474 | 7:-95180835 | MsG0780041790.01.T01:CDS |
| AGCGAATAAAGCGGGCCAGA+AGG | 0.567952 | 7:+95180800 | None:intergenic |
| AGCAAGTTTAGCGAATAAAG+CGG | 0.583424 | 7:+95180791 | None:intergenic |
| GTCATCTTCGAGGATGAGAA+GGG | 0.616441 | 7:+95180887 | None:intergenic |
| TTGATTGCCAGTGCTGCTGG+TGG | 0.634125 | 7:-95180747 | MsG0780041790.01.T01:CDS |
| TGAGAAGAAACCAGAGCCAG+AGG | 0.638704 | 7:-95180643 | MsG0780041790.01.T01:CDS |
| AGAGGAAGAAGAGAGTGACG+AGG | 0.679954 | 7:-95180625 | MsG0780041790.01.T01:CDS |
| TGTAGTGTGAAAATGTCGCT+CGG | 0.684963 | 7:-95180930 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGCTGAGAAGAAAAATATTA+GGG | - | Chr7:95180742-95180761 | MsG0780041790.01.T01:CDS | 25.0% |
| ! | TTGCTGAGAAGAAAAATATT+AGG | - | Chr7:95180741-95180760 | MsG0780041790.01.T01:CDS | 25.0% |
| AACTTTGTTGAAATCCGCAA+AGG | - | Chr7:95180679-95180698 | MsG0780041790.01.T01:CDS | 35.0% | |
| AGCAAGTTTAGCGAATAAAG+CGG | + | Chr7:95180726-95180745 | None:intergenic | 35.0% | |
| CAACAAAGTTGTGATGTTGT+CGG | + | Chr7:95180669-95180688 | None:intergenic | 35.0% | |
| GCAAGTTTAGCGAATAAAGC+GGG | + | Chr7:95180725-95180744 | None:intergenic | 40.0% | |
| GGATTCAACATCAACCTTTG+CGG | + | Chr7:95180696-95180715 | None:intergenic | 40.0% | |
| !! | AGGTTGATGTTGAATCCTTC+TGG | - | Chr7:95180699-95180718 | MsG0780041790.01.T01:CDS | 40.0% |
| AGAAGAGAGTGACGAGGAAA+TGG | - | Chr7:95180895-95180914 | MsG0780041790.01.T01:CDS | 45.0% | |
| AGTGACGAGGAAATGGGATT+CGG | - | Chr7:95180902-95180921 | MsG0780041790.01.T01:CDS | 45.0% | |
| CTGGAATGTTGTCATCTTCG+AGG | + | Chr7:95180640-95180659 | None:intergenic | 45.0% | |
| GAAGAGAGTGACGAGGAAAT+GGG | - | Chr7:95180896-95180915 | MsG0780041790.01.T01:CDS | 45.0% | |
| GTCATCTTCGAGGATGAGAA+GGG | + | Chr7:95180630-95180649 | None:intergenic | 45.0% | |
| TGTCATCTTCGAGGATGAGA+AGG | + | Chr7:95180631-95180650 | None:intergenic | 45.0% | |
| AGAGGAAGAAGAGAGTGACG+AGG | - | Chr7:95180889-95180908 | MsG0780041790.01.T01:CDS | 50.0% | |
| GATTTGATTGCCAGTGCTGC+TGG | - | Chr7:95180764-95180783 | MsG0780041790.01.T01:CDS | 50.0% | |
| TCGTCACTCTCTTCTTCCTC+TGG | + | Chr7:95180890-95180909 | None:intergenic | 50.0% | |
| TGAGAAGAAACCAGAGCCAG+AGG | - | Chr7:95180871-95180890 | MsG0780041790.01.T01:CDS | 50.0% | |
| AGCGAATAAAGCGGGCCAGA+AGG | + | Chr7:95180717-95180736 | None:intergenic | 55.0% | |
| GTGATGTTGTCGGCAGTTGC+TGG | + | Chr7:95180659-95180678 | None:intergenic | 55.0% | |
| TTCTTCTCAGCAGCAGCAGC+TGG | + | Chr7:95180860-95180879 | None:intergenic | 55.0% | |
| ! | CTCTCTTCTTCCTCTGGCTC+TGG | + | Chr7:95180884-95180903 | None:intergenic | 55.0% |
| ! | TTGATTGCCAGTGCTGCTGG+TGG | - | Chr7:95180767-95180786 | MsG0780041790.01.T01:CDS | 55.0% |
| CCACCACCTGATGCTGCAAC+TGG | + | Chr7:95180824-95180843 | None:intergenic | 60.0% | |
| CCAGTTGCAGCATCAGGTGG+TGG | - | Chr7:95180821-95180840 | MsG0780041790.01.T01:CDS | 60.0% | |
| GCTCCAGTTGCAGCATCAGG+TGG | - | Chr7:95180818-95180837 | MsG0780041790.01.T01:CDS | 60.0% | |
| GCTGCTCCAGTTGCAGCATC+AGG | - | Chr7:95180815-95180834 | MsG0780041790.01.T01:CDS | 60.0% | |
| ! | ATTGCCAGTGCTGCTGGTGG+TGG | - | Chr7:95180770-95180789 | MsG0780041790.01.T01:CDS | 60.0% |
| AGCACCACCACCAGCAGCAC+TGG | + | Chr7:95180777-95180796 | None:intergenic | 65.0% | |
| AGCAGCAGCTGGAGCAGCAG+CGG | + | Chr7:95180849-95180868 | None:intergenic | 65.0% | |
| AGCAGCCGCAACAGCTGCTC+CGG | + | Chr7:95180801-95180820 | None:intergenic | 65.0% | |
| TGCTCCCGGAGCAGCTGTTG+CGG | - | Chr7:95180793-95180812 | MsG0780041790.01.T01:CDS | 65.0% | |
| GCAGCCGCAACAGCTGCTCC+GGG | + | Chr7:95180800-95180819 | None:intergenic | 70.0% | |
| !! | GCTGCTGGTGGTGGTGCTCC+CGG | - | Chr7:95180779-95180798 | MsG0780041790.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 95180596 | 95180940 | 95180596 | ID=MsG0780041790.01;Name=MsG0780041790.01 |
| Chr7 | mRNA | 95180596 | 95180940 | 95180596 | ID=MsG0780041790.01.T01;Parent=MsG0780041790.01;Name=MsG0780041790.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|114 |
| Chr7 | exon | 95180596 | 95180940 | 95180596 | ID=MsG0780041790.01.T01:exon:22763;Parent=MsG0780041790.01.T01 |
| Chr7 | CDS | 95180596 | 95180940 | 95180596 | ID=MsG0780041790.01.T01:cds;Parent=MsG0780041790.01.T01 |
| Gene Sequence |
| Protein sequence |
