AlfalfaGEDB | MsG0480023465.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023465.01

KEGG: K07910
GO: GO:0003924 GO:0005525
PFAM: PF00071

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023465.01.T01	XP_024629521.1	71.958	189	13	3	1	163	1	175	6.11E-80	246

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023465.01.T01	O23657	62.434	189	31	3	1	163	1	175	6.58E-70	213

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023465.01.T01	G7LE30	71.958	189	13	3	1	163	1	175	2.92e-80	246

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048197.01	MsG0480023465.01	0.803550	3.304594e-49	1.665512e-46
MsG0080048843.01	MsG0480023465.01	0.812573	3.961713e-51	2.527866e-48
MsG0080048968.01	MsG0480023465.01	0.802405	5.698451e-49	2.789151e-46
MsG0180001043.01	MsG0480023465.01	0.813026	3.151714e-51	2.035293e-48
MsG0180003586.01	MsG0480023465.01	0.802501	5.445479e-49	2.671662e-46
MsG0180004016.01	MsG0480023465.01	0.805594	1.238000e-49	6.575898e-47
MsG0180005106.01	MsG0480023465.01	0.812432	4.253193e-51	2.703933e-48
MsG0280007549.01	MsG0480023465.01	0.812746	3.629590e-51	2.326601e-48
MsG0280008286.01	MsG0480023465.01	0.818181	2.241167e-52	1.665751e-49
MsG0280010416.01	MsG0480023465.01	0.802406	5.695047e-49	2.787573e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023465.01.T01	MTR_8g093740	71.958	189	13	3	1	163	1	175	7.40e-84	246
MsG0480023465.01.T01	MTR_3g096350	65.079	189	25	4	1	163	1	174	2.05e-72	216
MsG0480023465.01.T01	MTR_1g029450	60.773	181	31	3	9	163	13	179	2.64e-67	204
MsG0480023465.01.T01	MTR_8g093740	62.252	151	17	3	39	163	6	142	1.90e-50	160
MsG0480023465.01.T01	MTR_1g090717	48.187	193	56	5	1	163	1	179	1.07e-48	156
MsG0480023465.01.T01	MTR_7g103070	48.619	181	52	5	10	163	13	179	2.92e-48	155
MsG0480023465.01.T01	MTR_1g062760	48.333	180	53	4	10	163	11	176	1.03e-47	154
MsG0480023465.01.T01	MTR_3g096350	76.190	105	10	2	1	105	1	90	3.08e-46	148
MsG0480023465.01.T01	MTR_3g096350	76.190	105	10	2	1	105	1	90	3.19e-46	148
MsG0480023465.01.T01	MTR_2g005500	40.884	181	68	3	8	163	4	170	8.84e-36	123

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023465.01.T01	AT1G43890	62.434	189	31	3	1	163	1	175	6.71e-71	213
MsG0480023465.01.T01	AT1G43890	62.434	189	31	3	1	163	1	175	6.71e-71	213
MsG0480023465.01.T01	AT1G43890	62.434	189	31	3	1	163	1	175	6.71e-71	213
MsG0480023465.01.T01	AT5G03530	50.794	189	53	4	1	163	1	175	5.60e-55	172
MsG0480023465.01.T01	AT5G03530	50.794	189	53	4	1	163	1	175	5.60e-55	172
MsG0480023465.01.T01	AT3G09910	48.947	190	57	5	1	164	1	176	1.47e-44	145
MsG0480023465.01.T01	AT3G09910	48.947	190	57	5	1	164	1	176	1.47e-44	145
MsG0480023465.01.T01	AT3G09910	48.677	189	57	5	1	163	1	175	3.86e-44	145
MsG0480023465.01.T01	AT3G09910	48.677	189	57	5	1	163	1	175	3.86e-44	145
MsG0480023465.01.T01	AT3G09910	48.677	189	57	5	1	163	1	175	3.86e-44	145

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Find 34 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTTAAGCTTGCCATT+TGG	0.383397	4:+85600036	MsG0480023465.01.T01:CDS
AAAGCTTAAGCTTGCCATTT+GGG	0.317285	4:+85600037	MsG0480023465.01.T01:CDS
AAAGGAAACGAACCAATGGT+GGG	0.650141	4:-85599897	None:intergenic
AAGAGGGAATAGACTTTGCT+AGG	0.457004	4:+85602375	MsG0480023465.01.T01:CDS
AAGCTTGCCATTTGGGATAC+TGG	0.289194	4:+85600044	MsG0480023465.01.T01:CDS
ACCAAATAATGCAGCTGGTC+AGG	0.352646	4:+85600419	MsG0480023465.01.T01:intron
AGAAAATGGATTCGAGTTCA+GGG	0.507840	4:+85599766	MsG0480023465.01.T01:exon
AGAGGGAATAGACTTTGCTA+GGG	0.577586	4:+85602376	MsG0480023465.01.T01:CDS
AGAGTTGTGACAAAGAAAGA+GGG	0.538991	4:+85602359	MsG0480023465.01.T01:CDS
AGGGGCTCAAGGAATCATCA+TGG	0.573145	4:+85600476	MsG0480023465.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGCCAATTGTATATATG+GGG	+	Chr4:85600334-85600353	MsG0480023465.01.T01:intron	30.0%
AAGAATTGAGAGGCAAAAAA+GGG	-	Chr4:85600517-85600536	None:intergenic	30.0%
ACTGACATGATTTGAATACA+AGG	-	Chr4:85601384-85601403	None:intergenic	30.0%
CAACTTAATTGCATAACAGA+TGG	-	Chr4:85601734-85601753	None:intergenic	30.0%
CATACAAATCATGAATTGCA+TGG	+	Chr4:85602131-85602150	MsG0480023465.01.T01:intron	30.0%
CCAATAATTAATCTTTAGCG+GGG	+	Chr4:85600209-85600228	MsG0480023465.01.T01:intron	30.0%
GCAATCTGTTTAATGATCTT+TGG	+	Chr4:85601094-85601113	MsG0480023465.01.T01:intron	30.0%
GTAAAAGAAGACAATGAAGA+AGG	+	Chr4:85602070-85602089	MsG0480023465.01.T01:intron	30.0%
TAAAAGTTATCATTTCCGGA+GGG	-	Chr4:85601909-85601928	None:intergenic	30.0%
TAAACTTTATCATTTCCGGA+GGG	-	Chr4:85602003-85602022	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	85599613	85602487	85599613	ID=MsG0480023465.01;Name=MsG0480023465.01
Chr4	mRNA	85599613	85602487	85599613	ID=MsG0480023465.01.T01;Parent=MsG0480023465.01;Name=MsG0480023465.01.T01;_AED=0.50;_eAED=0.58;_QI=50\|0.4\|0.33\|1\|0.2\|0.16\|6\|0\|164
Chr4	exon	85599613	85599653	85599613	ID=MsG0480023465.01.T01:exon:2885;Parent=MsG0480023465.01.T01
Chr4	exon	85599762	85599906	85599762	ID=MsG0480023465.01.T01:exon:2886;Parent=MsG0480023465.01.T01
Chr4	exon	85599997	85600069	85599997	ID=MsG0480023465.01.T01:exon:2887;Parent=MsG0480023465.01.T01
Chr4	exon	85600143	85600180	85600143	ID=MsG0480023465.01.T01:exon:2888;Parent=MsG0480023465.01.T01
Chr4	exon	85600433	85600542	85600433	ID=MsG0480023465.01.T01:exon:2889;Parent=MsG0480023465.01.T01
Chr4	exon	85602350	85602487	85602350	ID=MsG0480023465.01.T01:exon:2890;Parent=MsG0480023465.01.T01
Chr4	five_prime_UTR	85599613	85599653	85599613	ID=MsG0480023465.01.T01:five_prime_utr;Parent=MsG0480023465.01.T01
Chr4	five_prime_UTR	85599762	85599770	85599762	ID=MsG0480023465.01.T01:five_prime_utr;Parent=MsG0480023465.01.T01
Chr4	CDS	85599771	85599906	85599771	ID=MsG0480023465.01.T01:cds;Parent=MsG0480023465.01.T01
Chr4	CDS	85599997	85600069	85599997	ID=MsG0480023465.01.T01:cds;Parent=MsG0480023465.01.T01
Chr4	CDS	85600143	85600180	85600143	ID=MsG0480023465.01.T01:cds;Parent=MsG0480023465.01.T01
Chr4	CDS	85600433	85600542	85600433	ID=MsG0480023465.01.T01:cds;Parent=MsG0480023465.01.T01
Chr4	CDS	85602350	85602487	85602350	ID=MsG0480023465.01.T01:cds;Parent=MsG0480023465.01.T01

Gene Sequence

>MsG0480023465.01.T01

ATGGATTCGAGTTCAGGGCAGCAAGAATTCGATTATATGTTCAAATTGTTAATGATTGGGGATTCTGGTGTTGGCAAAAGTAGTCTTCTTCTCAGATTCACCTCAGATGATTTTGATGATCTCTCTCCCACCATTGGTGTTGATTTTAAGGTCAAATATGTCACGATAGAGGGTAAAAAGCTTAAGCTTGCCATTTGGGATACTGGTATATTGGAGTTTTATTTATTTTGTAATCTTGTTATTGGTGCTGGTCAGGAGAGATTTAGAACACTGACAAGTTCTTACTACAGAGGGGCTCAAGGAATCATCATGGGCAAGTTCCTATTCTCCCTTTTTTGCCTCTCAATTCTTCCAATAGAGGGTGATAGAGTTGTGACAAAGAAAGAGGGAATAGACTTTGCTAGGGAATATGGTTGCCTATTTATTGAATGCAGTGCTAAAACTCGAGTAAATGTGCAGCAATGCTTTGAAGAGCTTGTTTTGAAGGTACATTAA

Protein sequence

>MsG0480023465.01.T01

MDSSSGQQEFDYMFKLLMIGDSGVGKSSLLLRFTSDDFDDLSPTIGVDFKVKYVTIEGKKLKLAIWDTGILEFYLFCNLVIGAGQERFRTLTSSYYRGAQGIIMGKFLFSLFCLSILPIEGDRVVTKKEGIDFAREYGCLFIECSAKTRVNVQQCFEELVLKVH*