AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022183.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022183.01.T01 MTR_4g083490 98.876 89 1 0 10 98 66 154 1.64e-60 184
MsG0480022183.01.T01 MTR_2g028790 91.011 89 8 0 10 98 68 156 1.68e-56 174
MsG0480022183.01.T01 MTR_4g022470 80.899 89 17 0 10 98 10 98 3.40e-50 154
MsG0480022183.01.T01 MTR_4g022570 80.899 89 17 0 10 98 64 152 9.11e-50 157
MsG0480022183.01.T01 MTR_1g047710 77.528 89 20 0 10 98 12 100 1.90e-49 153
MsG0480022183.01.T01 MTR_1g047720 78.652 89 19 0 10 98 64 152 1.93e-48 154
MsG0480022183.01.T01 MTR_7g058900 77.273 88 20 0 11 98 65 152 4.52e-46 147
MsG0480022183.01.T01 MTR_7g058640 69.663 89 27 0 10 98 64 152 1.44e-43 140
MsG0480022183.01.T01 MTR_7g058640 69.663 89 27 0 10 98 64 152 4.53e-43 140
MsG0480022183.01.T01 MTR_2g436870 68.182 88 28 0 10 97 64 151 7.15e-40 132
MsG0480022183.01.T01 MTR_7g058640 73.684 76 20 0 25 100 1 76 5.34e-39 127
MsG0480022183.01.T01 MTR_1g111890 52.336 107 32 2 11 98 65 171 2.63e-33 115
MsG0480022183.01.T01 MTR_5g089370 38.824 85 50 2 14 98 66 148 3.41e-15 68.2
MsG0480022183.01.T01 MTR_4g115390 37.647 85 51 2 14 98 70 152 1.65e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022183.01.T01 AT2G33120 78.889 90 19 0 9 98 71 160 1.03e-49 157
MsG0480022183.01.T01 AT2G32670 80.899 89 17 0 10 98 129 217 1.45e-49 158
MsG0480022183.01.T01 AT2G33120 79.775 89 18 0 10 98 64 152 4.21e-49 155
MsG0480022183.01.T01 AT1G04760 79.775 89 18 0 10 98 64 152 7.54e-49 155
MsG0480022183.01.T01 AT1G04760 79.775 89 18 0 10 98 64 152 7.54e-49 155
MsG0480022183.01.T01 AT1G04750 78.652 89 19 0 10 98 64 152 9.10e-49 154
MsG0480022183.01.T01 AT2G33110 71.591 88 25 0 11 98 65 152 1.01e-43 140
MsG0480022183.01.T01 AT2G33110 70.000 90 27 0 9 98 63 152 4.68e-43 140
MsG0480022183.01.T01 AT1G04750 82.432 74 13 0 25 98 1 74 2.04e-41 133
MsG0480022183.01.T01 AT1G04750 82.432 74 13 0 25 98 1 74 2.04e-41 133
MsG0480022183.01.T01 AT1G04750 82.432 74 13 0 25 98 1 74 2.04e-41 133
MsG0480022183.01.T01 AT4G15780 68.182 88 28 0 10 97 65 152 7.26e-40 130
MsG0480022183.01.T01 AT3G54300 51.852 108 31 2 11 98 65 171 2.61e-31 110
MsG0480022183.01.T01 AT3G54300 51.852 108 31 2 11 98 65 171 2.61e-31 110
MsG0480022183.01.T01 AT3G54300 51.852 108 31 2 11 98 65 171 2.61e-31 110
MsG0480022183.01.T01 AT5G11150 38.824 85 50 2 14 98 67 149 1.60e-15 69.3
MsG0480022183.01.T01 AT5G11150 38.824 85 50 2 14 98 67 149 1.60e-15 69.3
MsG0480022183.01.T01 AT4G32150 37.647 85 51 2 14 98 66 148 6.55e-15 67.8
MsG0480022183.01.T01 AT5G22360 36.264 91 54 3 9 98 62 149 3.03e-14 65.9

Find 26 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATATGTTCCTTCAATTT+TGG 0.127814 4:-69377104 None:intergenic
AACAGCTTGAATAAGGAATT+TGG 0.135464 4:+69376993 MsG0480022183.01.T01:CDS
TGCACCTAACAGCTTGAATA+AGG 0.338054 4:+69376986 MsG0480022183.01.T01:CDS
ACAGCTTGAATAAGGAATTT+GGG 0.392757 4:+69376994 MsG0480022183.01.T01:CDS
GCAAGCTTACTCACCTCCTC+CGG 0.394354 4:-69377143 None:intergenic
AATTCCTTATTCAAGCTGTT+AGG 0.425518 4:-69376990 None:intergenic
TTGTGGCAGGCCAAAATTGA+AGG 0.466510 4:+69377094 MsG0480022183.01.T01:intron
CATGATGGAGAATATTGAAA+AGG 0.507884 4:+69377202 MsG0480022183.01.T01:CDS
TTGTGTTGTTGCGGATGAAA+CGG 0.512801 4:+69376878 MsG0480022183.01.T01:CDS
GCTTGCTAAGGTGAAGGCTC+AGG 0.546240 4:+69377160 MsG0480022183.01.T01:CDS
CAAGCTTACTCACCTCCTCC+GGG 0.549863 4:-69377142 None:intergenic
AACGGTTGGAAGACAAGTGC+CGG 0.560243 4:+69376896 MsG0480022183.01.T01:CDS
TTTGTTGCGAAATATGGTGG+TGG 0.569761 4:+69376948 MsG0480022183.01.T01:CDS
TTGTGTTGATCACCCGGAGG+AGG 0.583937 4:+69377130 MsG0480022183.01.T01:CDS
GCAGTATTGTGTTGATCACC+CGG 0.590880 4:+69377124 MsG0480022183.01.T01:CDS
GAGTAAGCTTGCTAAGGTGA+AGG 0.597551 4:+69377154 MsG0480022183.01.T01:CDS
TTGTTGCGAAATATGGTGGT+GGG 0.599780 4:+69376949 MsG0480022183.01.T01:CDS
TGAAGTGAAAGGTGTCATGA+TGG 0.602006 4:+69377187 MsG0480022183.01.T01:CDS
GGAGGTGAGTAAGCTTGCTA+AGG 0.602293 4:+69377148 MsG0480022183.01.T01:CDS
GTTGTTGCGGATGAAACGGT+TGG 0.620353 4:+69376882 MsG0480022183.01.T01:CDS
GCTCAGGTGTCTGAAGTGAA+AGG 0.620623 4:+69377176 MsG0480022183.01.T01:CDS
TGTAGCGTATTGTGTTGTTG+CGG 0.643271 4:+69376869 MsG0480022183.01.T01:CDS
TGCGAAATATGGTGGTGGGA+AGG 0.648574 4:+69376953 MsG0480022183.01.T01:CDS
GGTTGGAAGACAAGTGCCGG+TGG 0.663656 4:+69376899 MsG0480022183.01.T01:CDS
ACACGTTCCAGAAAAGCCAC+CGG 0.669559 4:-69376915 None:intergenic
GTATTGTGTTGATCACCCGG+AGG 0.776651 4:+69377127 MsG0480022183.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTGATTTGATATTTAATTG+TGG + Chr4:69377077-69377096 MsG0480022183.01.T01:intron 20.0%
! ATTTGATATTTAATTGTGGC+AGG + Chr4:69377081-69377100 MsG0480022183.01.T01:intron 25.0%
!! GAGTTTATGATGATGATTTA+GGG + Chr4:69377021-69377040 MsG0480022183.01.T01:intron 25.0%
!! TGAGTTTATGATGATGATTT+AGG + Chr4:69377020-69377039 MsG0480022183.01.T01:intron 25.0%
AACAGCTTGAATAAGGAATT+TGG + Chr4:69376993-69377012 MsG0480022183.01.T01:CDS 30.0%
AATTCCTTATTCAAGCTGTT+AGG - Chr4:69376993-69377012 None:intergenic 30.0%
ACAGCTTGAATAAGGAATTT+GGG + Chr4:69376994-69377013 MsG0480022183.01.T01:CDS 30.0%
! CATGATGGAGAATATTGAAA+AGG + Chr4:69377202-69377221 MsG0480022183.01.T01:CDS 30.0%
! GATGATTTTGTTGCGAAATA+TGG + Chr4:69376942-69376961 MsG0480022183.01.T01:CDS 30.0%
! TGCATATGTTCCTTCAATTT+TGG - Chr4:69377107-69377126 None:intergenic 30.0%
!!! TTGCGATTTTTGTTGTTGAT+GGG + Chr4:69377047-69377066 MsG0480022183.01.T01:intron 30.0%
! GATTTTGTTGCGAAATATGG+TGG + Chr4:69376945-69376964 MsG0480022183.01.T01:CDS 35.0%
!!! GTTGCGATTTTTGTTGTTGA+TGG + Chr4:69377046-69377065 MsG0480022183.01.T01:intron 35.0%
TGCACCTAACAGCTTGAATA+AGG + Chr4:69376986-69377005 MsG0480022183.01.T01:CDS 40.0%
TTGTTGCGAAATATGGTGGT+GGG + Chr4:69376949-69376968 MsG0480022183.01.T01:CDS 40.0%
TTTGTTGCGAAATATGGTGG+TGG + Chr4:69376948-69376967 MsG0480022183.01.T01:CDS 40.0%
! TGTAGCGTATTGTGTTGTTG+CGG + Chr4:69376869-69376888 MsG0480022183.01.T01:CDS 40.0%
! TTGTGTTGTTGCGGATGAAA+CGG + Chr4:69376878-69376897 MsG0480022183.01.T01:CDS 40.0%
!! TGAAGTGAAAGGTGTCATGA+TGG + Chr4:69377187-69377206 MsG0480022183.01.T01:CDS 40.0%
TTGTGGCAGGCCAAAATTGA+AGG + Chr4:69377094-69377113 MsG0480022183.01.T01:intron 45.0%
! GCAGTATTGTGTTGATCACC+CGG + Chr4:69377124-69377143 MsG0480022183.01.T01:CDS 45.0%
!! GAGTAAGCTTGCTAAGGTGA+AGG + Chr4:69377154-69377173 MsG0480022183.01.T01:CDS 45.0%
AACGGTTGGAAGACAAGTGC+CGG + Chr4:69376896-69376915 MsG0480022183.01.T01:CDS 50.0%
ACACGTTCCAGAAAAGCCAC+CGG - Chr4:69376918-69376937 None:intergenic 50.0%
GTTGTTGCGGATGAAACGGT+TGG + Chr4:69376882-69376901 MsG0480022183.01.T01:CDS 50.0%
! GCTCAGGTGTCTGAAGTGAA+AGG + Chr4:69377176-69377195 MsG0480022183.01.T01:CDS 50.0%
! GTATTGTGTTGATCACCCGG+AGG + Chr4:69377127-69377146 MsG0480022183.01.T01:CDS 50.0%
!! GGAGGTGAGTAAGCTTGCTA+AGG + Chr4:69377148-69377167 MsG0480022183.01.T01:CDS 50.0%
!! TGCGAAATATGGTGGTGGGA+AGG + Chr4:69376953-69376972 MsG0480022183.01.T01:CDS 50.0%
!!! GGCTTTTCTGGAACGTGTGA+AGG + Chr4:69376920-69376939 MsG0480022183.01.T01:CDS 50.0%
CAAGCTTACTCACCTCCTCC+GGG - Chr4:69377145-69377164 None:intergenic 55.0%
GCAAGCTTACTCACCTCCTC+CGG - Chr4:69377146-69377165 None:intergenic 55.0%
! GCTTGCTAAGGTGAAGGCTC+AGG + Chr4:69377160-69377179 MsG0480022183.01.T01:CDS 55.0%
! TTGTGTTGATCACCCGGAGG+AGG + Chr4:69377130-69377149 MsG0480022183.01.T01:CDS 55.0%
!!! ACAAGTGCCGGTGGCTTTTC+TGG + Chr4:69376908-69376927 MsG0480022183.01.T01:CDS 55.0%
!! GGTTGGAAGACAAGTGCCGG+TGG + Chr4:69376899-69376918 MsG0480022183.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 69376846 69377247 69376846 ID=MsG0480022183.01;Name=MsG0480022183.01
Chr4 mRNA 69376846 69377247 69376846 ID=MsG0480022183.01.T01;Parent=MsG0480022183.01;Name=MsG0480022183.01.T01;_AED=0.50;_eAED=0.57;_QI=0|1|0|1|1|1|2|0|104
Chr4 exon 69376846 69377015 69376846 ID=MsG0480022183.01.T01:exon:8301;Parent=MsG0480022183.01.T01
Chr4 exon 69377103 69377247 69377103 ID=MsG0480022183.01.T01:exon:8302;Parent=MsG0480022183.01.T01
Chr4 CDS 69376846 69377015 69376846 ID=MsG0480022183.01.T01:cds;Parent=MsG0480022183.01.T01
Chr4 CDS 69377103 69377247 69377103 ID=MsG0480022183.01.T01:cds;Parent=MsG0480022183.01.T01
Gene Sequence

>MsG0480022183.01.T01

TTGAGAGTAATTTCTTGTGTGATTGTAGCGTATTGTGTTGTTGCGGATGAAACGGTTGGAAGACAAGTGCCGGTGGCTTTTCTGGAACGTGTGAAGGATGATTTTGTTGCGAAATATGGTGGTGGGAAGGCGTCTACAGCTGCACCTAACAGCTTGAATAAGGAATTTGGGCCAAAATTGAAGGAACATATGCAGTATTGTGTTGATCACCCGGAGGAGGTGAGTAAGCTTGCTAAGGTGAAGGCTCAGGTGTCTGAAGTGAAAGGTGTCATGATGGAGAATATTGAAAAGGTATACTCTTCTACTTTAACCTAA

Protein sequence

>MsG0480022183.01.T01

LRVISCVIVAYCVVADETVGRQVPVAFLERVKDDFVAKYGGGKASTAAPNSLNKEFGPKLKEHMQYCVDHPEEVSKLAKVKAQVSEVKGVMMENIEKVYSSTLT*