AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018332.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018332.01.T01 MTR_4g010050 97.872 141 3 0 4 144 213 353 1.93e-94 281
MsG0480018332.01.T01 MTR_2g104050 84.672 137 21 0 4 140 213 349 3.89e-78 239
MsG0480018332.01.T01 MTR_7g085470 64.407 118 31 2 4 116 189 300 8.62e-42 141
MsG0480018332.01.T01 MTR_7g085570 45.217 115 26 2 2 116 188 265 1.75e-21 88.2
MsG0480018332.01.T01 MTR_7g104890 30.882 136 94 0 5 140 146 281 3.16e-18 80.1
MsG0480018332.01.T01 MTR_7g104890 30.882 136 94 0 5 140 279 414 4.38e-18 80.1
MsG0480018332.01.T01 MTR_8g032340 36.709 79 50 0 5 83 289 367 7.13e-12 62.0
MsG0480018332.01.T01 MTR_8g032480 35.366 82 53 0 5 86 114 195 8.60e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018332.01.T01 AT1G48900 87.234 141 18 0 4 144 213 353 2.96e-84 253
MsG0480018332.01.T01 AT1G48900 87.234 141 18 0 4 144 213 353 4.01e-83 253
MsG0480018332.01.T01 AT5G49500 82.270 141 25 0 4 144 93 233 1.51e-80 242
MsG0480018332.01.T01 AT5G49500 82.270 141 25 0 4 144 215 355 3.99e-79 242
MsG0480018332.01.T01 AT1G15310 74.468 141 35 1 4 144 213 352 7.02e-69 215
MsG0480018332.01.T01 AT5G03940 31.618 136 93 0 5 140 289 424 8.48e-19 82.0
MsG0480018332.01.T01 AT2G45770 36.709 79 50 0 5 83 284 362 1.67e-11 61.2

Find 38 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATTATATTAAACATAAT+AGG 0.211600 4:+3455967 None:intergenic
CCATTTGTCAGTCGTCTATT+AGG 0.261155 4:-3456337 MsG0480018332.01.T01:intron
AAATTATATTAAACATAATA+GGG 0.281770 4:+3455968 None:intergenic
TCAGGCCAGGGAGACTTGTC+TGG 0.286825 4:-3456137 MsG0480018332.01.T01:intron
TGCAGCTTATTAAGCAGTTC+AGG 0.332627 4:+3456071 None:intergenic
CAACACTCTGTTTAAATGCT+TGG 0.344126 4:+3456665 None:intergenic
GATCCATTGGAACAACTTCA+TGG 0.399514 4:+3456099 None:intergenic
AACACTCTGTTTAAATGCTT+GGG 0.406606 4:+3456666 None:intergenic
GTTTCAGAATATTCTTAAGA+TGG 0.414472 4:-3456012 MsG0480018332.01.T01:CDS
CCTGTCATATTCATTGGAAC+TGG 0.418342 4:-3456397 MsG0480018332.01.T01:CDS
AAGAGTCCTGTCATATTCAT+TGG 0.428242 4:-3456403 MsG0480018332.01.T01:CDS
TTTGTCATGGATAGCAGTAT+TGG 0.450799 4:-3456709 MsG0480018332.01.T01:CDS
TTAAACAGAGTGTTGCAGTT+GGG 0.452491 4:-3456657 MsG0480018332.01.T01:CDS
AGATCTTGTTATCTTTGTCA+TGG 0.480044 4:-3456722 MsG0480018332.01.T01:CDS
TTTCAGAATATTCTTAAGAT+GGG 0.482656 4:-3456011 MsG0480018332.01.T01:CDS
CATGGATAGCAGTATTGGTC+AGG 0.485878 4:-3456704 MsG0480018332.01.T01:CDS
ATGACAAAGATAACAAGATC+TGG 0.486961 4:+3456724 None:intergenic
GTACATAATCCTCAAGGTAA+AGG 0.502143 4:+3456040 None:intergenic
TAAACAGAGTGTTGCAGTTG+GGG 0.502364 4:-3456656 MsG0480018332.01.T01:CDS
AATTATATTAAACATAATAG+GGG 0.506718 4:+3455969 None:intergenic
AGTTCAGGCTGTTGATCCAT+TGG 0.517338 4:+3456086 None:intergenic
CCTAATAGACGACTGACAAA+TGG 0.523880 4:+3456337 None:intergenic
CAGGCCAGGGAGACTTGTCT+GGG 0.527597 4:-3456136 MsG0480018332.01.T01:CDS
AATCCATGAAGTTGTTCCAA+TGG 0.540168 4:-3456102 MsG0480018332.01.T01:CDS
AAAATGGATGGCCATGCAAA+GGG 0.540546 4:-3456619 MsG0480018332.01.T01:CDS
GATGGCCATGCAAAGGGTGG+TGG 0.543431 4:-3456613 MsG0480018332.01.T01:CDS
CCAGTTCCAATGAATATGAC+AGG 0.549067 4:+3456397 None:intergenic
GCGCACCACCACCCTTTGCA+TGG 0.554323 4:+3456608 None:intergenic
TGAACCCAGACAAGTCTCCC+TGG 0.565640 4:+3456132 None:intergenic
TTTAAACAGAGTGTTGCAGT+TGG 0.570851 4:-3456658 MsG0480018332.01.T01:CDS
CATTGGAACTGGAGAACACA+TGG 0.583180 4:-3456386 MsG0480018332.01.T01:CDS
CTGCTTAATAAGCTGCAAGA+AGG 0.584033 4:-3456065 MsG0480018332.01.T01:CDS
AGATCTGGTTTCTGAATCAA+CGG 0.586337 4:+3456739 None:intergenic
AAGAAGGAACCTTTACCTTG+AGG 0.616437 4:-3456049 MsG0480018332.01.T01:CDS
ATTATATTAAACATAATAGG+GGG 0.617978 4:+3455970 None:intergenic
CTGCTCGTACATAATCCTCA+AGG 0.625136 4:+3456034 None:intergenic
TAAGATGGGTCCCATGAGCG+AGG 0.637224 4:-3455997 MsG0480018332.01.T01:CDS
ATGGATGGCCATGCAAAGGG+TGG 0.687249 4:-3456616 MsG0480018332.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAATAGGATTATATC+AGG + Chr4:3456142-3456161 None:intergenic 15.0%
!! AACATCATATTTATATAAGT+TGG + Chr4:3456178-3456197 None:intergenic 15.0%
!! TTAATATACATGCTTGAAAA+AGG + Chr4:3456505-3456524 None:intergenic 20.0%
!! TTTCAGAATATTCTTAAGAT+GGG - Chr4:3456696-3456715 MsG0480018332.01.T01:CDS 20.0%
!!! ATGATGATGATAACAAAAAA+AGG + Chr4:3456232-3456251 None:intergenic 20.0%
! AAGTATATACATGCTTGAAA+AGG + Chr4:3456444-3456463 None:intergenic 25.0%
! GATTAATATAAAGGGAAAAC+TGG + Chr4:3456532-3456551 None:intergenic 25.0%
! GTTTCAGAATATTCTTAAGA+TGG - Chr4:3456695-3456714 MsG0480018332.01.T01:CDS 25.0%
!! TTCAAGCATGTATATACTTT+TGG - Chr4:3456445-3456464 MsG0480018332.01.T01:intron 25.0%
!!! ATTTTTTTTGCTTAGTGTTG+CGG - Chr4:3456276-3456295 MsG0480018332.01.T01:intron 25.0%
AACACTCTGTTTAAATGCTT+GGG + Chr4:3456044-3456063 None:intergenic 30.0%
ACATATCCTAAAACCAATGT+TGG + Chr4:3456479-3456498 None:intergenic 30.0%
ATATACATGCTTGAAAAAGG+TGG + Chr4:3456502-3456521 None:intergenic 30.0%
ATGACAAAGATAACAAGATC+TGG + Chr4:3455986-3456005 None:intergenic 30.0%
CATATCCTAAAACCAATGTT+GGG + Chr4:3456478-3456497 None:intergenic 30.0%
CCTTTATATTAATCTCCAGT+CGG - Chr4:3456538-3456557 MsG0480018332.01.T01:intron 30.0%
CGACTGGAGATTAATATAAA+GGG + Chr4:3456540-3456559 None:intergenic 30.0%
GTCATTATCACAAAAATGGA+TGG - Chr4:3456076-3456095 MsG0480018332.01.T01:CDS 30.0%
! AGATCTTGTTATCTTTGTCA+TGG - Chr4:3455985-3456004 MsG0480018332.01.T01:CDS 30.0%
AAGAGTCCTGTCATATTCAT+TGG - Chr4:3456304-3456323 MsG0480018332.01.T01:intron 35.0%
AATCCATGAAGTTGTTCCAA+TGG - Chr4:3456605-3456624 MsG0480018332.01.T01:CDS 35.0%
CAACACTCTGTTTAAATGCT+TGG + Chr4:3456045-3456064 None:intergenic 35.0%
CATATCCTAAAACCAATGTC+GGG + Chr4:3456416-3456435 None:intergenic 35.0%
CCGACTGGAGATTAATATAA+AGG + Chr4:3456541-3456560 None:intergenic 35.0%
GCATATCCTAAAACCAATGT+CGG + Chr4:3456417-3456436 None:intergenic 35.0%
GGCTGTCATTATCACAAAAA+TGG - Chr4:3456072-3456091 MsG0480018332.01.T01:CDS 35.0%
GTACATAATCCTCAAGGTAA+AGG + Chr4:3456670-3456689 None:intergenic 35.0%
TATACATGCTTGAAAAGGTG+AGG + Chr4:3456439-3456458 None:intergenic 35.0%
TTTGTCATGGATAGCAGTAT+TGG - Chr4:3455998-3456017 MsG0480018332.01.T01:CDS 35.0%
! GTCTATTAGGTGTGTACTTT+TGG - Chr4:3456383-3456402 MsG0480018332.01.T01:CDS 35.0%
! TTAATCTCCAGTCGGTATTT+TGG - Chr4:3456546-3456565 MsG0480018332.01.T01:intron 35.0%
!! TTAAACAGAGTGTTGCAGTT+GGG - Chr4:3456050-3456069 MsG0480018332.01.T01:CDS 35.0%
!! TTTAAACAGAGTGTTGCAGT+TGG - Chr4:3456049-3456068 MsG0480018332.01.T01:CDS 35.0%
!!! TTTGGTTTTTGTCCCAACAT+TGG - Chr4:3456463-3456482 MsG0480018332.01.T01:intron 35.0%
!!! TTTGTCCCAACATTGGTTTT+AGG - Chr4:3456470-3456489 MsG0480018332.01.T01:intron 35.0%
AAAAATGGATGGCCATGCAA+AGG - Chr4:3456087-3456106 MsG0480018332.01.T01:CDS 40.0%
AAAATGGATGGCCATGCAAA+GGG - Chr4:3456088-3456107 MsG0480018332.01.T01:CDS 40.0%
AAGAAGGAACCTTTACCTTG+AGG - Chr4:3456658-3456677 MsG0480018332.01.T01:CDS 40.0%
CCAGTTCCAATGAATATGAC+AGG + Chr4:3456313-3456332 None:intergenic 40.0%
CCATTTGTCAGTCGTCTATT+AGG - Chr4:3456370-3456389 MsG0480018332.01.T01:CDS 40.0%
CCTAATAGACGACTGACAAA+TGG + Chr4:3456373-3456392 None:intergenic 40.0%
CCTGTCATATTCATTGGAAC+TGG - Chr4:3456310-3456329 MsG0480018332.01.T01:intron 40.0%
CTGCTTAATAAGCTGCAAGA+AGG - Chr4:3456642-3456661 MsG0480018332.01.T01:CDS 40.0%
GATCCATTGGAACAACTTCA+TGG + Chr4:3456611-3456630 None:intergenic 40.0%
TGCAGCTTATTAAGCAGTTC+AGG + Chr4:3456639-3456658 None:intergenic 40.0%
!! TAAACAGAGTGTTGCAGTTG+GGG - Chr4:3456051-3456070 MsG0480018332.01.T01:CDS 40.0%
AGTTCAGGCTGTTGATCCAT+TGG + Chr4:3456624-3456643 None:intergenic 45.0%
CATTGGAACTGGAGAACACA+TGG - Chr4:3456321-3456340 MsG0480018332.01.T01:intron 45.0%
CTGCTCGTACATAATCCTCA+AGG + Chr4:3456676-3456695 None:intergenic 45.0%
TATCACGACACAGCAAACAC+AGG + Chr4:3456208-3456227 None:intergenic 45.0%
! TTTGGTTTCTGTCCCGACAT+TGG - Chr4:3456401-3456420 MsG0480018332.01.T01:CDS 45.0%
!! CATGGATAGCAGTATTGGTC+AGG - Chr4:3456003-3456022 MsG0480018332.01.T01:CDS 45.0%
!! TCCAGTCGGTATTTTGGATC+AGG - Chr4:3456552-3456571 MsG0480018332.01.T01:intron 45.0%
!!! TCTGTCCCGACATTGGTTTT+AGG - Chr4:3456408-3456427 MsG0480018332.01.T01:CDS 45.0%
!! TTATATCATATAAATATAAT+AGG + Chr4:3456153-3456172 None:intergenic 5.0%
GCCTGATCCAAAATACCGAC+TGG + Chr4:3456556-3456575 None:intergenic 50.0%
!! CGGTATTTTGGATCAGGCCA+GGG - Chr4:3456558-3456577 MsG0480018332.01.T01:intron 50.0%
!! TCGGTATTTTGGATCAGGCC+AGG - Chr4:3456557-3456576 MsG0480018332.01.T01:intron 50.0%
!!! TGGTCAGGCTGCTTTTGATC+AGG - Chr4:3456018-3456037 MsG0480018332.01.T01:CDS 50.0%
AGGGGGTTAACCTCGCTCAT+GGG + Chr4:3456723-3456742 None:intergenic 55.0%
ATGGATGGCCATGCAAAGGG+TGG - Chr4:3456091-3456110 MsG0480018332.01.T01:CDS 55.0%
TAAGATGGGTCCCATGAGCG+AGG - Chr4:3456710-3456729 MsG0480018332.01.T01:CDS 55.0%
TAGGGGGTTAACCTCGCTCA+TGG + Chr4:3456724-3456743 None:intergenic 55.0%
TGAACCCAGACAAGTCTCCC+TGG + Chr4:3456578-3456597 None:intergenic 55.0%
CAGGCCAGGGAGACTTGTCT+GGG - Chr4:3456571-3456590 MsG0480018332.01.T01:intron 60.0%
GATGGCCATGCAAAGGGTGG+TGG - Chr4:3456094-3456113 MsG0480018332.01.T01:CDS 60.0%
TCAGGCCAGGGAGACTTGTC+TGG - Chr4:3456570-3456589 MsG0480018332.01.T01:intron 60.0%
GCGCACCACCACCCTTTGCA+TGG + Chr4:3456102-3456121 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 3455971 3456758 3455971 ID=MsG0480018332.01;Name=MsG0480018332.01
Chr4 mRNA 3455971 3456758 3455971 ID=MsG0480018332.01.T01;Parent=MsG0480018332.01;Name=MsG0480018332.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|3|0|147
Chr4 exon 3456601 3456758 3456601 ID=MsG0480018332.01.T01:exon:3722;Parent=MsG0480018332.01.T01
Chr4 exon 3456338 3456438 3456338 ID=MsG0480018332.01.T01:exon:3721;Parent=MsG0480018332.01.T01
Chr4 exon 3455971 3456155 3455971 ID=MsG0480018332.01.T01:exon:3720;Parent=MsG0480018332.01.T01
Chr4 CDS 3456601 3456758 3456601 ID=MsG0480018332.01.T01:cds;Parent=MsG0480018332.01.T01
Chr4 CDS 3456338 3456438 3456338 ID=MsG0480018332.01.T01:cds;Parent=MsG0480018332.01.T01
Chr4 CDS 3455971 3456155 3455971 ID=MsG0480018332.01.T01:cds;Parent=MsG0480018332.01.T01
Gene Sequence

>MsG0480018332.01.T01

TTGATTCAGAAACCAGATCTTGTTATCTTTGTCATGGATAGCAGTATTGGTCAGGCTGCTTTTGATCAGGCCCAAGCATTTAAACAGAGTGTTGCAGTTGGGGCTGTCATTATCACAAAAATGGATGGCCATGCAAAGGGTGGTGGTGCGCTTAGTGCTGTTGCGGCTACAAAGAGTCCTGTCATATTCATTGGAACTGGAGAACACATGGATGAATTTGAAGTTTTTGATGTTAAACCATTTGTCAGTCGTCTATTAGGCCAGGGAGACTTGTCTGGGTTCATGAACAAAATCCATGAAGTTGTTCCAATGGATCAACAGCCTGAACTGCTTAATAAGCTGCAAGAAGGAACCTTTACCTTGAGGATTATGTACGAGCAGTTTCAGAATATTCTTAAGATGGGTCCCATGAGCGAGGTTAACCCCCTATTATGTTTAATATAA

Protein sequence

>MsG0480018332.01.T01

LIQKPDLVIFVMDSSIGQAAFDQAQAFKQSVAVGAVIITKMDGHAKGGGALSAVAATKSPVIFIGTGEHMDEFEVFDVKPFVSRLLGQGDLSGFMNKIHEVVPMDQQPELLNKLQEGTFTLRIMYEQFQNILKMGPMSEVNPLLCLI*