Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024833.01.T01 | GAU17371.1 | 96.117 | 103 | 4 | 0 | 1 | 103 | 42 | 144 | 3.86E-62 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024833.01.T01 | Q9ZQE6 | 51.515 | 99 | 47 | 1 | 4 | 102 | 45 | 142 | 1.44E-26 | 99.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024833.01.T01 | A0A2Z6LYI2 | 96.117 | 103 | 4 | 0 | 1 | 103 | 42 | 144 | 1.84e-62 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000114.01 | MsG0580024833.01 | 0.813317 | 2.721212e-51 | 1.771209e-48 |
| MsG0180000854.01 | MsG0580024833.01 | 0.823664 | 1.226613e-53 | 1.063006e-50 |
| MsG0180001100.01 | MsG0580024833.01 | 0.816362 | 5.750242e-52 | 4.066133e-49 |
| MsG0180001880.01 | MsG0580024833.01 | 0.809137 | 2.195755e-50 | 1.278912e-47 |
| MsG0180002799.01 | MsG0580024833.01 | 0.809438 | 1.892298e-50 | 1.111019e-47 |
| MsG0180003357.01 | MsG0580024833.01 | 0.813647 | 2.302226e-51 | 1.512019e-48 |
| MsG0180006104.01 | MsG0580024833.01 | 0.827940 | 1.185110e-54 | 1.160982e-51 |
| MsG0280011073.01 | MsG0580024833.01 | 0.815706 | 8.059155e-52 | 5.597468e-49 |
| MsG0280011401.01 | MsG0580024833.01 | 0.806637 | 7.466397e-50 | 4.073006e-47 |
| MsG0380013050.01 | MsG0580024833.01 | -0.802630 | 5.121192e-49 | 2.520895e-46 |
| MsG0380014866.01 | MsG0580024833.01 | 0.824656 | 7.171158e-54 | 6.392726e-51 |
| MsG0480021739.01 | MsG0580024833.01 | 0.804321 | 2.285308e-49 | 1.174745e-46 |
| MsG0480023381.01 | MsG0580024833.01 | 0.804250 | 2.364533e-49 | 1.213207e-46 |
| MsG0480023578.01 | MsG0580024833.01 | 0.814173 | 1.763100e-51 | 1.174518e-48 |
| MsG0480023641.01 | MsG0580024833.01 | 0.808944 | 2.415069e-50 | 1.399293e-47 |
| MsG0480023978.01 | MsG0580024833.01 | 0.818456 | 1.942137e-52 | 1.454606e-49 |
| MsG0580024829.01 | MsG0580024833.01 | 0.907365 | 5.480824e-81 | 9.693320e-77 |
| MsG0580024830.01 | MsG0580024833.01 | 0.850975 | 1.198855e-60 | 2.390214e-57 |
| MsG0580024833.01 | MsG0580025625.01 | 0.802492 | 5.467197e-49 | 2.681739e-46 |
| MsG0580024833.01 | MsG0580029065.01 | 0.828461 | 8.874404e-55 | 8.827134e-52 |
| MsG0580024833.01 | MsG0580029919.01 | 0.800717 | 1.264412e-48 | 5.929045e-46 |
| MsG0580024833.01 | MsG0680030515.01 | 0.802164 | 6.387546e-49 | 3.107186e-46 |
| MsG0580024833.01 | MsG0680034235.01 | 0.840330 | 9.280869e-58 | 1.317456e-54 |
| MsG0580024833.01 | MsG0680035307.01 | 0.803493 | 3.395835e-49 | 1.708923e-46 |
| MsG0580024833.01 | MsG0780036117.01 | 0.814641 | 1.389107e-51 | 9.372248e-49 |
| MsG0580024833.01 | MsG0780037330.01 | 0.819052 | 1.421752e-52 | 1.082465e-49 |
| MsG0580024833.01 | MsG0780037399.01 | 0.806295 | 8.816879e-50 | 4.768177e-47 |
| MsG0580024833.01 | MsG0780037701.01 | 0.817314 | 3.516246e-52 | 2.551976e-49 |
| MsG0580024833.01 | MsG0780039994.01 | 0.802015 | 6.855855e-49 | 3.322312e-46 |
| MsG0580024833.01 | MsG0780041187.01 | 0.805807 | 1.116690e-49 | 5.964338e-47 |
| MsG0580024833.01 | MsG0880047267.01 | 0.804675 | 1.927533e-49 | 9.999352e-47 |
| MsG0580024833.01 | MsG0880047519.01 | 0.865225 | 6.890044e-65 | 2.224764e-61 |
| MsG0380015745.01 | MsG0580024833.01 | 0.824031 | 1.006065e-53 | 8.808306e-51 |
| MsG0380016740.01 | MsG0580024833.01 | 0.834594 | 2.738162e-56 | 3.264681e-53 |
| MsG0380017229.01 | MsG0580024833.01 | 0.800812 | 1.209186e-48 | 5.683693e-46 |
| MsG0280006634.01 | MsG0580024833.01 | 0.807151 | 5.813349e-50 | 3.213866e-47 |
| MsG0280006669.01 | MsG0580024833.01 | 0.828372 | 9.327494e-55 | 9.253481e-52 |
| MsG0280008932.01 | MsG0580024833.01 | 0.862015 | 6.833660e-64 | 1.971654e-60 |
| MsG0280010158.01 | MsG0580024833.01 | 0.819669 | 1.028921e-52 | 7.968997e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024833.01.T01 | MTR_5g017550 | 93.204 | 103 | 7 | 0 | 1 | 103 | 40 | 142 | 1.52e-63 | 191 |
| MsG0580024833.01.T01 | MTR_5g017510 | 94.231 | 104 | 5 | 1 | 1 | 103 | 40 | 143 | 2.44e-62 | 188 |
| MsG0580024833.01.T01 | MTR_5g017560 | 94.231 | 104 | 5 | 1 | 1 | 103 | 40 | 143 | 2.44e-62 | 188 |
| MsG0580024833.01.T01 | MTR_5g017540 | 82.143 | 84 | 15 | 0 | 1 | 84 | 15 | 98 | 4.31e-44 | 139 |
| MsG0580024833.01.T01 | MTR_4g086260 | 53.000 | 100 | 46 | 1 | 3 | 102 | 51 | 149 | 1.60e-27 | 100 |
| MsG0580024833.01.T01 | MTR_3g067610 | 37.624 | 101 | 60 | 1 | 7 | 107 | 3 | 100 | 1.61e-16 | 70.1 |
| MsG0580024833.01.T01 | MTR_8g105230 | 39.604 | 101 | 58 | 2 | 9 | 107 | 36 | 135 | 7.57e-15 | 67.0 |
| MsG0580024833.01.T01 | MTR_8g107110 | 36.893 | 103 | 63 | 1 | 8 | 108 | 23 | 125 | 9.48e-15 | 66.2 |
| MsG0580024833.01.T01 | MTR_8g107110 | 36.735 | 98 | 56 | 2 | 7 | 101 | 58 | 152 | 5.91e-12 | 58.9 |
| MsG0580024833.01.T01 | MTR_5g079340 | 37.500 | 96 | 60 | 0 | 8 | 103 | 3 | 98 | 3.19e-14 | 64.3 |
| MsG0580024833.01.T01 | MTR_4g067270 | 38.000 | 100 | 61 | 1 | 8 | 106 | 4 | 103 | 6.85e-14 | 63.5 |
| MsG0580024833.01.T01 | MTR_6g079570 | 34.000 | 100 | 65 | 1 | 8 | 106 | 25 | 124 | 1.63e-13 | 63.2 |
| MsG0580024833.01.T01 | MTR_1g032070 | 39.175 | 97 | 56 | 2 | 8 | 102 | 45 | 140 | 2.78e-12 | 60.5 |
| MsG0580024833.01.T01 | MTR_1g047100 | 35.922 | 103 | 62 | 2 | 8 | 107 | 3 | 104 | 3.25e-12 | 59.3 |
| MsG0580024833.01.T01 | MTR_7g089760 | 40.404 | 99 | 58 | 1 | 9 | 106 | 5 | 103 | 3.31e-12 | 59.3 |
| MsG0580024833.01.T01 | MTR_4g122260 | 32.692 | 104 | 65 | 3 | 4 | 102 | 11 | 114 | 1.02e-11 | 58.2 |
| MsG0580024833.01.T01 | MTR_1g046950 | 33.333 | 102 | 65 | 1 | 8 | 106 | 3 | 104 | 1.03e-11 | 57.8 |
| MsG0580024833.01.T01 | MTR_2g098890 | 37.755 | 98 | 58 | 1 | 8 | 102 | 63 | 160 | 4.65e-11 | 57.4 |
| MsG0580024833.01.T01 | MTR_7g075040 | 37.736 | 106 | 53 | 3 | 8 | 102 | 46 | 149 | 6.27e-11 | 56.6 |
| MsG0580024833.01.T01 | MTR_1g041285 | 36.735 | 98 | 55 | 2 | 9 | 102 | 12 | 106 | 7.15e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024833.01.T01 | AT2G15680 | 51.515 | 99 | 47 | 1 | 4 | 102 | 45 | 142 | 1.47e-27 | 99.8 |
| MsG0580024833.01.T01 | AT3G03400 | 42.708 | 96 | 50 | 2 | 13 | 104 | 9 | 103 | 2.16e-17 | 72.4 |
| MsG0580024833.01.T01 | AT5G37770 | 39.000 | 100 | 56 | 1 | 8 | 102 | 16 | 115 | 8.29e-17 | 71.6 |
| MsG0580024833.01.T01 | AT5G37770 | 39.000 | 100 | 56 | 1 | 8 | 102 | 16 | 115 | 8.29e-17 | 71.6 |
| MsG0580024833.01.T01 | AT1G24620 | 43.564 | 101 | 54 | 2 | 9 | 107 | 37 | 136 | 1.65e-16 | 71.2 |
| MsG0580024833.01.T01 | AT5G17470 | 42.857 | 98 | 53 | 2 | 13 | 107 | 6 | 103 | 1.89e-16 | 70.5 |
| MsG0580024833.01.T01 | AT2G36180 | 44.118 | 102 | 53 | 2 | 10 | 107 | 1 | 102 | 2.72e-16 | 69.7 |
| MsG0580024833.01.T01 | AT1G18210 | 35.052 | 97 | 60 | 1 | 6 | 102 | 20 | 113 | 8.68e-14 | 63.9 |
| MsG0580024833.01.T01 | AT1G18210 | 35.052 | 97 | 60 | 1 | 6 | 102 | 20 | 113 | 8.68e-14 | 63.9 |
| MsG0580024833.01.T01 | AT3G59440 | 37.755 | 98 | 55 | 2 | 9 | 103 | 51 | 145 | 8.94e-14 | 64.3 |
| MsG0580024833.01.T01 | AT1G76650 | 33.333 | 102 | 68 | 0 | 6 | 107 | 40 | 141 | 1.39e-13 | 63.5 |
| MsG0580024833.01.T01 | AT1G76650 | 33.333 | 102 | 68 | 0 | 6 | 107 | 40 | 141 | 1.39e-13 | 63.5 |
| MsG0580024833.01.T01 | AT1G66400 | 37.000 | 100 | 56 | 2 | 8 | 102 | 14 | 111 | 2.63e-13 | 62.4 |
| MsG0580024833.01.T01 | AT2G43290 | 37.000 | 100 | 57 | 1 | 9 | 102 | 65 | 164 | 3.88e-13 | 63.2 |
| MsG0580024833.01.T01 | AT5G42380 | 36.634 | 101 | 62 | 2 | 8 | 106 | 48 | 148 | 1.24e-12 | 61.2 |
| MsG0580024833.01.T01 | AT3G03410 | 38.542 | 96 | 51 | 1 | 11 | 106 | 4 | 91 | 1.95e-12 | 59.7 |
| MsG0580024833.01.T01 | AT1G73630 | 35.294 | 102 | 62 | 2 | 2 | 102 | 12 | 110 | 2.33e-12 | 60.1 |
| MsG0580024833.01.T01 | AT4G14640 | 38.384 | 99 | 54 | 2 | 9 | 103 | 13 | 108 | 2.99e-12 | 59.7 |
| MsG0580024833.01.T01 | AT3G50360 | 37.755 | 98 | 60 | 1 | 6 | 102 | 24 | 121 | 3.99e-12 | 59.7 |
| MsG0580024833.01.T01 | AT1G05990 | 38.542 | 96 | 57 | 1 | 9 | 102 | 5 | 100 | 1.34e-11 | 57.8 |
| MsG0580024833.01.T01 | AT4G14640 | 36.735 | 98 | 55 | 2 | 9 | 102 | 13 | 107 | 1.76e-11 | 57.0 |
| MsG0580024833.01.T01 | AT2G17890 | 36.364 | 99 | 56 | 2 | 14 | 105 | 420 | 518 | 3.24e-11 | 58.9 |
| MsG0580024833.01.T01 | AT4G04700 | 33.673 | 98 | 65 | 0 | 10 | 107 | 337 | 434 | 4.01e-11 | 58.5 |
| MsG0580024833.01.T01 | AT2G17290 | 35.484 | 93 | 60 | 0 | 10 | 102 | 391 | 483 | 4.07e-11 | 58.5 |
| MsG0580024833.01.T01 | AT2G17290 | 35.484 | 93 | 60 | 0 | 10 | 102 | 391 | 483 | 4.07e-11 | 58.5 |
| MsG0580024833.01.T01 | AT3G22930 | 35.417 | 96 | 61 | 1 | 9 | 103 | 35 | 130 | 6.30e-11 | 55.8 |
| MsG0580024833.01.T01 | AT4G04695 | 32.653 | 98 | 66 | 0 | 10 | 107 | 337 | 434 | 8.99e-11 | 57.8 |
Find 28 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGACTTTAAAGAGTTTA+TGG | 0.095878 | 5:+10455106 | MsG0580024833.01.T01:CDS |
| CATTTCATTTCATCTTTCTT+TGG | 0.177414 | 5:-10454939 | None:intergenic |
| TTTCAAGTATTTGATATAAA+TGG | 0.216373 | 5:+10455182 | MsG0580024833.01.T01:CDS |
| TGATGCAGTGAAAGCATTTA+AGG | 0.222047 | 5:+10455058 | MsG0580024833.01.T01:CDS |
| CAAAGAAAGATGAAATGAAA+TGG | 0.359646 | 5:+10454940 | MsG0580024833.01.T01:CDS |
| GCTGCAAGAGCTTTGGATAA+GGG | 0.408921 | 5:+10455023 | MsG0580024833.01.T01:CDS |
| TAAAGCAGCTGCAAGAGCTT+TGG | 0.424129 | 5:+10455016 | MsG0580024833.01.T01:CDS |
| TCATTTCATCTTTCTTTGGT+TGG | 0.424902 | 5:-10454935 | None:intergenic |
| GAGCTTTGGATAAGGGGATT+GGG | 0.425029 | 5:+10455030 | MsG0580024833.01.T01:CDS |
| AGCTGCAAGAGCTTTGGATA+AGG | 0.430049 | 5:+10455022 | MsG0580024833.01.T01:CDS |
| CAAAGATGGCAAGATTAGTC+TGG | 0.440035 | 5:+10454986 | MsG0580024833.01.T01:CDS |
| GTCTCTCAATTTAAGAGGTT+AGG | 0.444024 | 5:+10455232 | MsG0580024833.01.T01:CDS |
| GAATTGTCTCTCAATTTAAG+AGG | 0.447161 | 5:+10455227 | MsG0580024833.01.T01:CDS |
| AGAGCTTTGGATAAGGGGAT+TGG | 0.465859 | 5:+10455029 | MsG0580024833.01.T01:CDS |
| AAAGAAAGATGAAATGAAAT+GGG | 0.477872 | 5:+10454941 | MsG0580024833.01.T01:CDS |
| GAGTTTATGGAAATGTTCAA+TGG | 0.480442 | 5:+10455119 | MsG0580024833.01.T01:CDS |
| CTGCAAGAGCTTTGGATAAG+GGG | 0.501691 | 5:+10455024 | MsG0580024833.01.T01:CDS |
| GCCATCTTTGTCAGAGTCCA+TGG | 0.508786 | 5:-10455081 | None:intergenic |
| AGCTTTGGATAAGGGGATTG+GGG | 0.547088 | 5:+10455031 | MsG0580024833.01.T01:CDS |
| GTATTTGATATAAATGGTGA+TGG | 0.547891 | 5:+10455188 | MsG0580024833.01.T01:CDS |
| ATGGAAATGTTCAATGGAGA+GGG | 0.552020 | 5:+10455125 | MsG0580024833.01.T01:CDS |
| GGGTAGTAAGATAAAAGAAG+AGG | 0.559838 | 5:+10455145 | MsG0580024833.01.T01:CDS |
| TATGGAAATGTTCAATGGAG+AGG | 0.569106 | 5:+10455124 | MsG0580024833.01.T01:CDS |
| AAATTCGACACAAACAAAGA+TGG | 0.592536 | 5:+10454972 | MsG0580024833.01.T01:CDS |
| AGTGAAAGCATTTAAGGCCA+TGG | 0.601115 | 5:+10455064 | MsG0580024833.01.T01:CDS |
| TATTTGATATAAATGGTGAT+GGG | 0.605312 | 5:+10455189 | MsG0580024833.01.T01:CDS |
| GCCATGGACTCTGACAAAGA+TGG | 0.639323 | 5:+10455080 | MsG0580024833.01.T01:CDS |
| TGATGGGAAAATCAGTGCTG+AGG | 0.731787 | 5:+10455205 | MsG0580024833.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCAAGTATTTGATATAAA+TGG | + | Chr5:10455182-10455201 | MsG0580024833.01.T01:CDS | 15.0% |
| !! | AAAGAAAGATGAAATGAAAT+GGG | + | Chr5:10454941-10454960 | MsG0580024833.01.T01:CDS | 20.0% |
| !! | TATTTGATATAAATGGTGAT+GGG | + | Chr5:10455189-10455208 | MsG0580024833.01.T01:CDS | 20.0% |
| ! | CAAAGAAAGATGAAATGAAA+TGG | + | Chr5:10454940-10454959 | MsG0580024833.01.T01:CDS | 25.0% |
| ! | CATTTCATTTCATCTTTCTT+TGG | - | Chr5:10454942-10454961 | None:intergenic | 25.0% |
| ! | GTATTTGATATAAATGGTGA+TGG | + | Chr5:10455188-10455207 | MsG0580024833.01.T01:CDS | 25.0% |
| !! | CATAGACTTTAAAGAGTTTA+TGG | + | Chr5:10455106-10455125 | MsG0580024833.01.T01:CDS | 25.0% |
| AAATTCGACACAAACAAAGA+TGG | + | Chr5:10454972-10454991 | MsG0580024833.01.T01:CDS | 30.0% | |
| GAATTGTCTCTCAATTTAAG+AGG | + | Chr5:10455227-10455246 | MsG0580024833.01.T01:CDS | 30.0% | |
| GAGTTTATGGAAATGTTCAA+TGG | + | Chr5:10455119-10455138 | MsG0580024833.01.T01:CDS | 30.0% | |
| TCATTTCATCTTTCTTTGGT+TGG | - | Chr5:10454938-10454957 | None:intergenic | 30.0% | |
| ATGGAAATGTTCAATGGAGA+GGG | + | Chr5:10455125-10455144 | MsG0580024833.01.T01:CDS | 35.0% | |
| GGGTAGTAAGATAAAAGAAG+AGG | + | Chr5:10455145-10455164 | MsG0580024833.01.T01:CDS | 35.0% | |
| TATGGAAATGTTCAATGGAG+AGG | + | Chr5:10455124-10455143 | MsG0580024833.01.T01:CDS | 35.0% | |
| !! | TGATGCAGTGAAAGCATTTA+AGG | + | Chr5:10455058-10455077 | MsG0580024833.01.T01:CDS | 35.0% |
| ! | CAAAGATGGCAAGATTAGTC+TGG | + | Chr5:10454986-10455005 | MsG0580024833.01.T01:CDS | 40.0% |
| !! | AGTGAAAGCATTTAAGGCCA+TGG | + | Chr5:10455064-10455083 | MsG0580024833.01.T01:CDS | 40.0% |
| TAAAGCAGCTGCAAGAGCTT+TGG | + | Chr5:10455016-10455035 | MsG0580024833.01.T01:CDS | 45.0% | |
| TGATGGGAAAATCAGTGCTG+AGG | + | Chr5:10455205-10455224 | MsG0580024833.01.T01:CDS | 45.0% | |
| !! | AGAGCTTTGGATAAGGGGAT+TGG | + | Chr5:10455029-10455048 | MsG0580024833.01.T01:CDS | 45.0% |
| !! | AGCTGCAAGAGCTTTGGATA+AGG | + | Chr5:10455022-10455041 | MsG0580024833.01.T01:CDS | 45.0% |
| !! | AGCTTTGGATAAGGGGATTG+GGG | + | Chr5:10455031-10455050 | MsG0580024833.01.T01:CDS | 45.0% |
| !! | CTGCAAGAGCTTTGGATAAG+GGG | + | Chr5:10455024-10455043 | MsG0580024833.01.T01:CDS | 45.0% |
| !! | GAGCTTTGGATAAGGGGATT+GGG | + | Chr5:10455030-10455049 | MsG0580024833.01.T01:CDS | 45.0% |
| !! | GCTGCAAGAGCTTTGGATAA+GGG | + | Chr5:10455023-10455042 | MsG0580024833.01.T01:CDS | 45.0% |
| GCCATCTTTGTCAGAGTCCA+TGG | - | Chr5:10455084-10455103 | None:intergenic | 50.0% | |
| GCCATGGACTCTGACAAAGA+TGG | + | Chr5:10455080-10455099 | MsG0580024833.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 10454927 | 10455253 | 10454927 | ID=MsG0580024833.01;Name=MsG0580024833.01 |
| Chr5 | mRNA | 10454927 | 10455253 | 10454927 | ID=MsG0580024833.01.T01;Parent=MsG0580024833.01;Name=MsG0580024833.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|108 |
| Chr5 | exon | 10454927 | 10455253 | 10454927 | ID=MsG0580024833.01.T01:exon:1953;Parent=MsG0580024833.01.T01 |
| Chr5 | CDS | 10454927 | 10455253 | 10454927 | ID=MsG0580024833.01.T01:cds;Parent=MsG0580024833.01.T01 |
| Gene Sequence |
| Protein sequence |
