AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002799.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002799.01.T01 MTR_4g008650 92.908 141 10 0 1 141 442 582 2.43e-86 270
MsG0180002799.01.T01 MTR_4g008650 92.908 141 10 0 1 141 442 582 4.88e-85 270
MsG0180002799.01.T01 MTR_8g090125 73.427 143 36 1 1 141 398 540 1.13e-65 216
MsG0180002799.01.T01 MTR_5g015590 56.738 141 59 1 1 141 446 584 3.12e-46 161
MsG0180002799.01.T01 MTR_5g015590 56.738 141 59 1 1 141 446 584 3.67e-46 161
MsG0180002799.01.T01 MTR_5g015590 56.738 141 59 1 1 141 446 584 4.48e-46 161
MsG0180002799.01.T01 MTR_5g015590 56.738 141 59 1 1 141 446 584 4.80e-46 160
MsG0180002799.01.T01 MTR_4g043690 61.379 145 52 2 1 141 445 589 4.67e-45 158
MsG0180002799.01.T01 MTR_0005s0140 61.379 145 52 2 1 141 360 504 5.33e-45 158
MsG0180002799.01.T01 MTR_8g446010 60.993 141 55 0 1 141 398 538 1.18e-44 157
MsG0180002799.01.T01 MTR_3g103070 54.610 141 64 0 1 141 271 411 4.56e-43 152
MsG0180002799.01.T01 MTR_3g103070 54.610 141 64 0 1 141 447 587 4.66e-43 152
MsG0180002799.01.T01 MTR_4g096990 55.319 141 59 1 1 141 446 582 4.80e-42 149
MsG0180002799.01.T01 MTR_8g045070 53.191 141 43 1 1 141 398 515 1.31e-33 125
MsG0180002799.01.T01 MTR_2g105830 38.732 142 81 2 1 141 493 629 2.39e-20 87.4
MsG0180002799.01.T01 MTR_4g008170 36.620 142 83 2 1 141 504 639 4.14e-20 86.7
MsG0180002799.01.T01 MTR_4g008170 36.620 142 83 2 1 141 511 646 5.02e-20 86.7
MsG0180002799.01.T01 MTR_2g038310 34.266 143 84 3 1 141 491 625 3.79e-17 78.2
MsG0180002799.01.T01 MTR_2g038310 34.266 143 84 3 1 141 491 625 4.30e-17 78.2
MsG0180002799.01.T01 MTR_6g016470 35.616 146 82 4 1 141 433 571 2.68e-16 75.9
MsG0180002799.01.T01 MTR_7g095710 34.483 145 84 4 1 141 461 598 2.60e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002799.01.T01 AT2G41560 65.957 141 47 1 1 141 340 479 7.08e-55 186
MsG0180002799.01.T01 AT2G41560 65.957 141 47 1 1 141 340 479 7.08e-55 186
MsG0180002799.01.T01 AT2G41560 65.957 141 47 1 1 141 340 479 7.08e-55 186
MsG0180002799.01.T01 AT2G41560 65.957 141 47 1 1 141 442 581 7.51e-55 186
MsG0180002799.01.T01 AT3G57330 62.411 141 49 1 1 141 387 523 3.89e-53 181
MsG0180002799.01.T01 AT3G57330 62.411 141 49 1 1 141 442 578 4.12e-53 181
MsG0180002799.01.T01 AT1G27770 53.191 141 66 0 1 141 384 524 6.81e-43 152
MsG0180002799.01.T01 AT1G27770 53.191 141 66 0 1 141 447 587 7.34e-43 152
MsG0180002799.01.T01 AT1G27770 53.191 141 66 0 1 141 447 587 7.34e-43 152
MsG0180002799.01.T01 AT1G27770 53.191 141 66 0 1 141 447 587 7.76e-43 152
MsG0180002799.01.T01 AT4G37640 52.817 142 64 2 1 141 445 584 8.10e-40 143
MsG0180002799.01.T01 AT2G22950 53.521 142 63 2 1 141 446 585 2.01e-39 142
MsG0180002799.01.T01 AT4G29900 36.879 141 83 2 1 141 473 607 1.36e-20 88.2
MsG0180002799.01.T01 AT4G29900 36.879 141 83 2 1 141 473 607 1.36e-20 88.2
MsG0180002799.01.T01 AT3G63380 38.194 144 79 5 1 141 444 580 2.51e-17 79.0
MsG0180002799.01.T01 AT5G57110 35.915 142 80 3 1 141 473 604 3.46e-17 78.6
MsG0180002799.01.T01 AT5G57110 35.915 142 80 3 1 141 473 604 3.46e-17 78.6
MsG0180002799.01.T01 AT5G57110 35.915 142 80 3 1 141 473 604 3.46e-17 78.6
MsG0180002799.01.T01 AT3G21180 30.282 142 92 2 1 141 486 621 9.10e-14 68.6
MsG0180002799.01.T01 AT3G22910 35.811 148 76 6 1 141 440 575 2.32e-11 61.6

Find 35 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGAATTTGGCTTGGTTTC+AGG 0.088234 1:-45457506 MsG0180002799.01.T01:CDS
ATATGGTGGTTGATAAAATT+TGG 0.156993 1:-45457709 MsG0180002799.01.T01:CDS
ATCAGAAATGTTGCCGATTT+GGG 0.249540 1:-45457354 MsG0180002799.01.T01:CDS
CATCAGAAATGTTGCCGATT+TGG 0.253064 1:-45457355 MsG0180002799.01.T01:CDS
GAATCAGCAATACTGGAATT+TGG 0.255403 1:-45457518 MsG0180002799.01.T01:CDS
CTCAACCTTAAGTACCTTAC+AGG 0.303221 1:+45457457 None:intergenic
AATCTCTGATTTCAACTTAT+CGG 0.303785 1:+45457646 None:intergenic
AGAAGGAAAACAGACAATAC+TGG 0.344901 1:-45457552 MsG0180002799.01.T01:CDS
ACCAACAGAATCAGCAATAC+TGG 0.363574 1:-45457525 MsG0180002799.01.T01:CDS
TATATTTCAGAATACTTCTT+CGG 0.415739 1:-45457591 MsG0180002799.01.T01:CDS
TTAATTCGAGGGAATGTCAT+TGG 0.422010 1:-45457326 MsG0180002799.01.T01:CDS
TAGATCCTGTAAGGTACTTA+AGG 0.436221 1:-45457462 MsG0180002799.01.T01:CDS
AGCAATACTGGAATTTGGCT+TGG 0.474761 1:-45457513 MsG0180002799.01.T01:CDS
AAAGCTCGGAACCCTCCATC+AGG 0.501465 1:+45457389 None:intergenic
TCTGAAATATAGCCTGCAAA+AGG 0.510920 1:+45457603 None:intergenic
GGGTTCCGAGCTTTCTGCAA+AGG 0.512141 1:-45457380 MsG0180002799.01.T01:CDS
TTCTTCCTGTCTGAATTGAA+AGG 0.527349 1:+45457434 None:intergenic
TGCACAGCATAGATCCTGTA+AGG 0.528305 1:-45457471 MsG0180002799.01.T01:CDS
CTAGTAGGTCTTCCTGATGG+AGG 0.535876 1:-45457401 MsG0180002799.01.T01:CDS
TCCAGTATTGCTGATTCTGT+TGG 0.540202 1:+45457524 None:intergenic
TCAGAAATGTTGCCGATTTG+GGG 0.547944 1:-45457353 MsG0180002799.01.T01:CDS
AAGAAGATGTCGGTGCTAGT+AGG 0.550515 1:-45457416 MsG0180002799.01.T01:CDS
AACGTTGACAAACAACCATA+TGG 0.552998 1:-45457726 MsG0180002799.01.T01:CDS
TTGAGCCTTTCAATTCAGAC+AGG 0.555436 1:-45457439 MsG0180002799.01.T01:CDS
ATGCACCTTTGCAGAAAGCT+CGG 0.556163 1:+45457375 None:intergenic
GAAGTAGTTAAAGACAAAGA+AGG 0.556974 1:-45457569 MsG0180002799.01.T01:CDS
GAAGGAAAACAGACAATACT+GGG 0.566868 1:-45457551 MsG0180002799.01.T01:CDS
TAGTAGGTCTTCCTGATGGA+GGG 0.573123 1:-45457400 MsG0180002799.01.T01:CDS
GAATTTGGCTTGGTTTCAGG+TGG 0.577510 1:-45457503 MsG0180002799.01.T01:CDS
GTGCTAGTAGGTCTTCCTGA+TGG 0.581135 1:-45457404 MsG0180002799.01.T01:CDS
CAGAAATGTTGCCGATTTGG+GGG 0.589263 1:-45457352 MsG0180002799.01.T01:CDS
GAAAAGACTGTGGAGATGAA+AGG 0.645618 1:-45457680 MsG0180002799.01.T01:CDS
TTCAGACAGGAAGAAGATGT+CGG 0.657425 1:-45457426 MsG0180002799.01.T01:CDS
TTGGATATGTGAAAAGACTG+TGG 0.658651 1:-45457690 MsG0180002799.01.T01:CDS
GTTGACAAACAACCATATGG+TGG 0.706114 1:-45457723 MsG0180002799.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTTCAGAATACTTCTT+CGG - Chr1:45457453-45457472 MsG0180002799.01.T01:CDS 20.0%
! AATCTCTGATTTCAACTTAT+CGG + Chr1:45457401-45457420 None:intergenic 25.0%
!! AAATTTTATCAACCACCATA+TGG + Chr1:45457336-45457355 None:intergenic 25.0%
!!! ATATGGTGGTTGATAAAATT+TGG - Chr1:45457335-45457354 MsG0180002799.01.T01:CDS 25.0%
GAAGTAGTTAAAGACAAAGA+AGG - Chr1:45457475-45457494 MsG0180002799.01.T01:CDS 30.0%
AACGTTGACAAACAACCATA+TGG - Chr1:45457318-45457337 MsG0180002799.01.T01:CDS 35.0%
AGAAGGAAAACAGACAATAC+TGG - Chr1:45457492-45457511 MsG0180002799.01.T01:CDS 35.0%
CGAATTAAAATCCCCCAAAT+CGG + Chr1:45457706-45457725 None:intergenic 35.0%
GAAGGAAAACAGACAATACT+GGG - Chr1:45457493-45457512 MsG0180002799.01.T01:CDS 35.0%
GAATCAGCAATACTGGAATT+TGG - Chr1:45457526-45457545 MsG0180002799.01.T01:CDS 35.0%
TTAATTCGAGGGAATGTCAT+TGG - Chr1:45457718-45457737 MsG0180002799.01.T01:CDS 35.0%
TTCTTCCTGTCTGAATTGAA+AGG + Chr1:45457613-45457632 None:intergenic 35.0%
TTGGATATGTGAAAAGACTG+TGG - Chr1:45457354-45457373 MsG0180002799.01.T01:CDS 35.0%
! TAGATCCTGTAAGGTACTTA+AGG - Chr1:45457582-45457601 MsG0180002799.01.T01:CDS 35.0%
! TCTGAAATATAGCCTGCAAA+AGG + Chr1:45457444-45457463 None:intergenic 35.0%
!! AATGTCATTGGTCGTTTTCT+TGG - Chr1:45457730-45457749 MsG0180002799.01.T01:CDS 35.0%
!! ATCAGAAATGTTGCCGATTT+GGG - Chr1:45457690-45457709 MsG0180002799.01.T01:CDS 35.0%
!! GAGATGCTCTCTTTTTGTTT+GGG - Chr1:45457752-45457771 MsG0180002799.01.T01:CDS 35.0%
!!! ATTTGGGGGATTTTAATTCG+AGG - Chr1:45457706-45457725 MsG0180002799.01.T01:CDS 35.0%
!!! TTTGGGGGATTTTAATTCGA+GGG - Chr1:45457707-45457726 MsG0180002799.01.T01:CDS 35.0%
ACCAACAGAATCAGCAATAC+TGG - Chr1:45457519-45457538 MsG0180002799.01.T01:CDS 40.0%
AGCAATACTGGAATTTGGCT+TGG - Chr1:45457531-45457550 MsG0180002799.01.T01:CDS 40.0%
CTCAACCTTAAGTACCTTAC+AGG + Chr1:45457590-45457609 None:intergenic 40.0%
GAAAAGACTGTGGAGATGAA+AGG - Chr1:45457364-45457383 MsG0180002799.01.T01:CDS 40.0%
GTTGACAAACAACCATATGG+TGG - Chr1:45457321-45457340 MsG0180002799.01.T01:CDS 40.0%
TTCAGACAGGAAGAAGATGT+CGG - Chr1:45457618-45457637 MsG0180002799.01.T01:CDS 40.0%
TTGAGCCTTTCAATTCAGAC+AGG - Chr1:45457605-45457624 MsG0180002799.01.T01:CDS 40.0%
! CATCAGAAATGTTGCCGATT+TGG - Chr1:45457689-45457708 MsG0180002799.01.T01:CDS 40.0%
! GGAGATGCTCTCTTTTTGTT+TGG - Chr1:45457751-45457770 MsG0180002799.01.T01:CDS 40.0%
!! TCAGAAATGTTGCCGATTTG+GGG - Chr1:45457691-45457710 MsG0180002799.01.T01:CDS 40.0%
!! TCCAGTATTGCTGATTCTGT+TGG + Chr1:45457523-45457542 None:intergenic 40.0%
!!! AGTTCTAAGCATCCTTTTGC+AGG - Chr1:45457429-45457448 MsG0180002799.01.T01:CDS 40.0%
ATGCACCTTTGCAGAAAGCT+CGG + Chr1:45457672-45457691 None:intergenic 45.0%
TAGTAGGTCTTCCTGATGGA+GGG - Chr1:45457644-45457663 MsG0180002799.01.T01:CDS 45.0%
TGCACAGCATAGATCCTGTA+AGG - Chr1:45457573-45457592 MsG0180002799.01.T01:CDS 45.0%
!! AAGAAGATGTCGGTGCTAGT+AGG - Chr1:45457628-45457647 MsG0180002799.01.T01:CDS 45.0%
!! CAGAAATGTTGCCGATTTGG+GGG - Chr1:45457692-45457711 MsG0180002799.01.T01:CDS 45.0%
!! CTGGAATTTGGCTTGGTTTC+AGG - Chr1:45457538-45457557 MsG0180002799.01.T01:CDS 45.0%
!! GAATTTGGCTTGGTTTCAGG+TGG - Chr1:45457541-45457560 MsG0180002799.01.T01:CDS 45.0%
CTAGTAGGTCTTCCTGATGG+AGG - Chr1:45457643-45457662 MsG0180002799.01.T01:CDS 50.0%
GTGCTAGTAGGTCTTCCTGA+TGG - Chr1:45457640-45457659 MsG0180002799.01.T01:CDS 50.0%
AAAGCTCGGAACCCTCCATC+AGG + Chr1:45457658-45457677 None:intergenic 55.0%
GGGTTCCGAGCTTTCTGCAA+AGG - Chr1:45457664-45457683 MsG0180002799.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 45457280 45457786 45457280 ID=MsG0180002799.01;Name=MsG0180002799.01
Chr1 mRNA 45457280 45457786 45457280 ID=MsG0180002799.01.T01;Parent=MsG0180002799.01;Name=MsG0180002799.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|168
Chr1 exon 45457280 45457786 45457280 ID=MsG0180002799.01.T01:exon:38643;Parent=MsG0180002799.01.T01
Chr1 CDS 45457280 45457786 45457280 ID=MsG0180002799.01.T01:cds;Parent=MsG0180002799.01.T01
Gene Sequence

>MsG0180002799.01.T01

ATGGGTTCAGCTAGTTGCATTTGCACTGATAAGACAGCAACGTTGACAAACAACCATATGGTGGTTGATAAAATTTGGATATGTGAAAAGACTGTGGAGATGAAAGGTGATGAAAGTACCGATAAGTTGAAATCAGAGATTTCTGATGAAGTTCTAAGCATCCTTTTGCAGGCTATATTTCAGAATACTTCTTCGGAAGTAGTTAAAGACAAAGAAGGAAAACAGACAATACTGGGAACACCAACAGAATCAGCAATACTGGAATTTGGCTTGGTTTCAGGTGGTGATTTCAATGCACAGCATAGATCCTGTAAGGTACTTAAGGTTGAGCCTTTCAATTCAGACAGGAAGAAGATGTCGGTGCTAGTAGGTCTTCCTGATGGAGGGTTCCGAGCTTTCTGCAAAGGTGCATCAGAAATGTTGCCGATTTGGGGGATTTTAATTCGAGGGAATGTCATTGGTCGTTTTCTTGGAGATGCTCTCTTTTTGTTTGGGAGAACGATTTGA

Protein sequence

>MsG0180002799.01.T01

MGSASCICTDKTATLTNNHMVVDKIWICEKTVEMKGDESTDKLKSEISDEVLSILLQAIFQNTSSEVVKDKEGKQTILGTPTESAILEFGLVSGGDFNAQHRSCKVLKVEPFNSDRKKMSVLVGLPDGGFRAFCKGASEMLPIWGILIRGNVIGRFLGDALFLFGRTI*