Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024830.01.T01 | PNX91353.1 | 73.438 | 64 | 17 | 0 | 14 | 77 | 33 | 96 | 2.33E-21 | 92.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024830.01.T01 | A0A2K3MKK1 | 73.438 | 64 | 17 | 0 | 14 | 77 | 33 | 96 | 1.11e-21 | 92.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001100.01 | MsG0580024830.01 | 0.809087 | 2.250623e-50 | 1.309086e-47 |
| MsG0180003357.01 | MsG0580024830.01 | 0.821018 | 5.046158e-53 | 4.058064e-50 |
| MsG0180005509.01 | MsG0580024830.01 | 0.814259 | 1.687528e-51 | 1.126838e-48 |
| MsG0180005960.01 | MsG0580024830.01 | 0.806980 | 6.318461e-50 | 3.477683e-47 |
| MsG0280010900.01 | MsG0580024830.01 | 0.812246 | 4.669959e-51 | 2.954038e-48 |
| MsG0280011073.01 | MsG0580024830.01 | 0.808739 | 2.671491e-50 | 1.539426e-47 |
| MsG0280011371.01 | MsG0580024830.01 | 0.800866 | 1.178768e-48 | 5.548723e-46 |
| MsG0380011669.01 | MsG0580024830.01 | 0.808593 | 2.870132e-50 | 1.647683e-47 |
| MsG0380013471.01 | MsG0580024830.01 | 0.805880 | 1.078052e-49 | 5.768754e-47 |
| MsG0380014727.01 | MsG0580024830.01 | 0.828524 | 8.571372e-55 | 8.541138e-52 |
| MsG0380014866.01 | MsG0580024830.01 | 0.820678 | 6.042459e-53 | 4.812675e-50 |
| MsG0480018847.01 | MsG0580024830.01 | 0.817922 | 2.564849e-52 | 1.892872e-49 |
| MsG0480019335.01 | MsG0580024830.01 | 0.802579 | 5.246898e-49 | 2.579393e-46 |
| MsG0480020024.01 | MsG0580024830.01 | 0.819738 | 9.919794e-53 | 7.697957e-50 |
| MsG0480023638.01 | MsG0580024830.01 | 0.841179 | 5.558245e-58 | 8.101089e-55 |
| MsG0580024829.01 | MsG0580024830.01 | 0.875040 | 4.214763e-68 | 1.940203e-64 |
| MsG0580024830.01 | MsG0580024833.01 | 0.850975 | 1.198855e-60 | 2.390214e-57 |
| MsG0580024830.01 | MsG0580024862.01 | 0.806627 | 7.504373e-50 | 4.092632e-47 |
| MsG0580024830.01 | MsG0580026032.01 | 0.810612 | 1.056872e-50 | 6.400404e-48 |
| MsG0580024830.01 | MsG0580027449.01 | 0.806305 | 8.772907e-50 | 4.745579e-47 |
| MsG0580024830.01 | MsG0680034235.01 | 0.843834 | 1.097712e-58 | 1.739015e-55 |
| MsG0580024830.01 | MsG0780036117.01 | 0.844282 | 8.324793e-59 | 1.337403e-55 |
| MsG0580024830.01 | MsG0780036183.01 | 0.811245 | 7.709939e-51 | 4.748191e-48 |
| MsG0580024830.01 | MsG0780037330.01 | 0.829736 | 4.356942e-55 | 4.498677e-52 |
| MsG0580024830.01 | MsG0780037701.01 | 0.806931 | 6.471599e-50 | 3.557416e-47 |
| MsG0580024830.01 | MsG0780038644.01 | 0.806284 | 8.863874e-50 | 4.792232e-47 |
| MsG0580024830.01 | MsG0780041210.01 | 0.811998 | 5.287107e-51 | 3.321855e-48 |
| MsG0580024830.01 | MsG0880042560.01 | 0.802570 | 5.269281e-49 | 2.589760e-46 |
| MsG0580024830.01 | MsG0880042622.01 | 0.842539 | 2.431779e-58 | 3.698933e-55 |
| MsG0580024830.01 | MsG0880045387.01 | 0.820565 | 6.414450e-53 | 5.093138e-50 |
| MsG0580024830.01 | MsG0880047267.01 | 0.812841 | 3.460582e-51 | 2.223626e-48 |
| MsG0380015745.01 | MsG0580024830.01 | 0.822000 | 2.993172e-53 | 2.474112e-50 |
| MsG0380016566.01 | MsG0580024830.01 | 0.803915 | 2.776010e-49 | 1.412304e-46 |
| MsG0380016740.01 | MsG0580024830.01 | 0.854991 | 8.511588e-62 | 1.936032e-58 |
| MsG0380017322.01 | MsG0580024830.01 | 0.806822 | 6.824175e-50 | 3.740713e-47 |
| MsG0380018022.01 | MsG0580024830.01 | 0.812675 | 3.762824e-51 | 2.407454e-48 |
| MsG0280006634.01 | MsG0580024830.01 | 0.808313 | 3.292568e-50 | 1.876508e-47 |
| MsG0280006669.01 | MsG0580024830.01 | 0.832835 | 7.535431e-56 | 8.522478e-53 |
| MsG0280007691.01 | MsG0580024830.01 | 0.802276 | 6.058039e-49 | 2.955345e-46 |
| MsG0280008932.01 | MsG0580024830.01 | 0.839203 | 1.823324e-57 | 2.499891e-54 |
| MsG0280010158.01 | MsG0580024830.01 | 0.830420 | 2.966341e-55 | 3.123563e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024830.01.T01 | MTR_5g017510 | 83.333 | 54 | 9 | 0 | 24 | 77 | 83 | 136 | 1.24e-23 | 89.0 |
| MsG0580024830.01.T01 | MTR_5g017560 | 83.333 | 54 | 9 | 0 | 24 | 77 | 83 | 136 | 1.24e-23 | 89.0 |
| MsG0580024830.01.T01 | MTR_5g017550 | 77.586 | 58 | 13 | 0 | 20 | 77 | 78 | 135 | 1.25e-23 | 89.0 |
| MsG0580024830.01.T01 | MTR_5g017540 | 63.830 | 47 | 17 | 0 | 12 | 58 | 45 | 91 | 6.27e-14 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGACTTTAAAGAGTTTA+TGG | 0.106408 | 5:-10437362 | MsG0580024830.01.T01:CDS |
| CAGCACTTTGGTGCCATTAA+TGG | 0.222231 | 5:-10437256 | MsG0580024830.01.T01:CDS |
| TAGCACTTTGGTGCCATTAA+TGG | 0.248676 | 5:+10437243 | None:intergenic |
| ATTCCTCAGCACTAATTTCC+GGG | 0.396318 | 5:+10437220 | None:intergenic |
| AATTCCTCAGCACTAATTTC+CGG | 0.410131 | 5:+10437219 | None:intergenic |
| TGGTGACAGTGATGCAGTTA+AGG | 0.436906 | 5:-10437419 | MsG0580024830.01.T01:CDS |
| GCTGTTGATTCTGACAAAGA+TGG | 0.456408 | 5:-10437388 | MsG0580024830.01.T01:CDS |
| AAGGCTTTGGATTAGGGTAA+TGG | 0.457061 | 5:-10437439 | MsG0580024830.01.T01:intron |
| TTTGCTAAACGTACCCGTAC+AGG | 0.459170 | 5:+10437975 | None:intergenic |
| AATGATCACGGGCAGCACTT+TGG | 0.480214 | 5:-10437268 | MsG0580024830.01.T01:CDS |
| GAGTTTATGGAAATGTTCAA+TGG | 0.480442 | 5:-10437349 | MsG0580024830.01.T01:CDS |
| CTAATTTCCGGGTAGCACTT+TGG | 0.483306 | 5:+10437231 | None:intergenic |
| ACAGGGTACTGCGCGGGTAC+TGG | 0.497715 | 5:+10437993 | None:intergenic |
| ATGTAGTGAGATAAAAGAAG+AGG | 0.544251 | 5:-10437323 | MsG0580024830.01.T01:CDS |
| TACCCGCGCAGTACCCTGTA+CGG | 0.575877 | 5:-10437989 | MsG0580024830.01.T01:CDS |
| GTATTTGATATAAATGATCA+CGG | 0.579244 | 5:-10437280 | MsG0580024830.01.T01:CDS |
| TTGCTAAACGTACCCGTACA+GGG | 0.598731 | 5:+10437976 | None:intergenic |
| AATGGCACCAAAGTGCTACC+CGG | 0.608225 | 5:-10437238 | MsG0580024830.01.T01:CDS |
| ACCCGTACAGGGTACTGCGC+GGG | 0.628664 | 5:+10437987 | None:intergenic |
| TATTTGATATAAATGATCAC+GGG | 0.630469 | 5:-10437279 | MsG0580024830.01.T01:CDS |
| ACCCGCGCAGTACCCTGTAC+GGG | 0.647016 | 5:-10437988 | MsG0580024830.01.T01:intron |
| TACCCGTACAGGGTACTGCG+CGG | 0.732488 | 5:+10437986 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTAGTTTAAAAACTCT+TGG | - | Chr5:10437399-10437418 | MsG0580024830.01.T01:CDS | 15.0% |
| !! | GTATTTGATATAAATGATCA+CGG | - | Chr5:10437977-10437996 | MsG0580024830.01.T01:intron | 20.0% |
| !! | TATTTGATATAAATGATCAC+GGG | - | Chr5:10437978-10437997 | MsG0580024830.01.T01:intron | 20.0% |
| !!! | AAGGAATTTTTGATACAATT+TGG | + | Chr5:10437569-10437588 | None:intergenic | 20.0% |
| !!! | TAGATATGATTAATTTGTAG+AGG | + | Chr5:10437461-10437480 | None:intergenic | 20.0% |
| ! | AAATTTGACACTAACATAGA+TGG | - | Chr5:10437761-10437780 | MsG0580024830.01.T01:intron | 25.0% |
| ! | CATTTCATTTCATCTTTCTA+CGG | + | Chr5:10437731-10437750 | None:intergenic | 25.0% |
| ! | GTAGAAAGATGAAATGAAAT+GGG | - | Chr5:10437730-10437749 | MsG0580024830.01.T01:intron | 25.0% |
| !! | CATAGACTTTAAAGAGTTTA+TGG | - | Chr5:10437895-10437914 | MsG0580024830.01.T01:intron | 25.0% |
| !!! | TAGAAAACTCAATTTTGACA+TGG | + | Chr5:10437600-10437619 | None:intergenic | 25.0% |
| !!! | TTGAGTTTTCTAAAGTTTCA+TGG | - | Chr5:10437608-10437627 | MsG0580024830.01.T01:intron | 25.0% |
| AATTTGTAGAGGAAAAGTAG+CGG | + | Chr5:10437450-10437469 | None:intergenic | 30.0% | |
| ATACTAGATTCTTAGTGTTG+TGG | + | Chr5:10437360-10437379 | None:intergenic | 30.0% | |
| ATGTAGTGAGATAAAAGAAG+AGG | - | Chr5:10437934-10437953 | MsG0580024830.01.T01:intron | 30.0% | |
| CGTAGAAAGATGAAATGAAA+TGG | - | Chr5:10437729-10437748 | MsG0580024830.01.T01:intron | 30.0% | |
| GAGTTTATGGAAATGTTCAA+TGG | - | Chr5:10437908-10437927 | MsG0580024830.01.T01:intron | 30.0% | |
| TTTCATAAGTTTCCTACTCT+TGG | + | Chr5:10437491-10437510 | None:intergenic | 30.0% | |
| ! | TTTTGACATGGTATCTTGTA+AGG | + | Chr5:10437588-10437607 | None:intergenic | 30.0% |
| AGTACGATTACGTGTTATGA+GGG | + | Chr5:10437542-10437561 | None:intergenic | 35.0% | |
| TAGTACGATTACGTGTTATG+AGG | + | Chr5:10437543-10437562 | None:intergenic | 35.0% | |
| TCATTTCATCTTTCTACGGT+TGG | + | Chr5:10437727-10437746 | None:intergenic | 35.0% | |
| !!! | TGTTCGGCTATATTTTTTCG+CGG | + | Chr5:10437659-10437678 | None:intergenic | 35.0% |
| CATAAGTTTCCTACTCTTGG+CGG | + | Chr5:10437488-10437507 | None:intergenic | 40.0% | |
| TACGGGTACGTTTAGCAAAA+TGG | - | Chr5:10437286-10437305 | MsG0580024830.01.T01:CDS | 40.0% | |
| TGACTTGAAGCTGTTCTGTT+CGG | + | Chr5:10437675-10437694 | None:intergenic | 40.0% | |
| !! | AAGGCTTTGGATTAGGGTAA+TGG | - | Chr5:10437818-10437837 | MsG0580024830.01.T01:intron | 40.0% |
| !! | GCTGTTGATTCTGACAAAGA+TGG | - | Chr5:10437869-10437888 | MsG0580024830.01.T01:intron | 40.0% |
| !! | GTGTTTAAAGCTCTGAAGCA+CGG | + | Chr5:10437317-10437336 | None:intergenic | 40.0% |
| !! | TAGCACTTTGGTGCCATTAA+TGG | + | Chr5:10438017-10438036 | None:intergenic | 40.0% |
| !! | TGTTTAAAGCTCTGAAGCAC+GGG | + | Chr5:10437316-10437335 | None:intergenic | 40.0% |
| !!! | ATATTTTTTCGCGGAGAGGT+TGG | + | Chr5:10437650-10437669 | None:intergenic | 40.0% |
| AGAGTACAAAGCAGCTGCAA+AGG | - | Chr5:10437799-10437818 | MsG0580024830.01.T01:intron | 45.0% | |
| CTAATTTCCGGGTAGCACTT+TGG | + | Chr5:10438029-10438048 | None:intergenic | 45.0% | |
| TGGTGACAGTGATGCAGTTA+AGG | - | Chr5:10437838-10437857 | MsG0580024830.01.T01:intron | 45.0% | |
| TTGCTAAACGTACCCGTACA+GGG | + | Chr5:10437284-10437303 | None:intergenic | 45.0% | |
| TTTGCTAAACGTACCCGTAC+AGG | + | Chr5:10437285-10437304 | None:intergenic | 45.0% | |
| !! | AGCTGCAAAGGCTTTGGATT+AGG | - | Chr5:10437811-10437830 | MsG0580024830.01.T01:intron | 45.0% |
| !! | CAGCACTTTGGTGCCATTAA+TGG | - | Chr5:10438001-10438020 | MsG0580024830.01.T01:CDS | 45.0% |
| !! | GCTGCAAAGGCTTTGGATTA+GGG | - | Chr5:10437812-10437831 | MsG0580024830.01.T01:intron | 45.0% |
| !!! | GGCTATATTTTTTCGCGGAG+AGG | + | Chr5:10437654-10437673 | None:intergenic | 45.0% |
| AATGATCACGGGCAGCACTT+TGG | - | Chr5:10437989-10438008 | MsG0580024830.01.T01:CDS | 50.0% | |
| CAAAGCAGCTGCAAAGGCTT+TGG | - | Chr5:10437805-10437824 | MsG0580024830.01.T01:intron | 50.0% | |
| ! | AATGGCACCAAAGTGCTACC+CGG | - | Chr5:10438019-10438038 | MsG0580024830.01.T01:CDS | 50.0% |
| ! | ATCTAGAGTCCGCCAAGAGT+AGG | - | Chr5:10437476-10437495 | MsG0580024830.01.T01:intron | 50.0% |
| !! | TTTTCGCGGAGAGGTTGGCT+TGG | + | Chr5:10437645-10437664 | None:intergenic | 55.0% |
| TACCCGCGCAGTACCCTGTA+CGG | - | Chr5:10437268-10437287 | MsG0580024830.01.T01:CDS | 60.0% | |
| !! | TACCCGTACAGGGTACTGCG+CGG | + | Chr5:10437274-10437293 | None:intergenic | 60.0% |
| ACCCGCGCAGTACCCTGTAC+GGG | - | Chr5:10437269-10437288 | MsG0580024830.01.T01:CDS | 65.0% | |
| !! | ACAGGGTACTGCGCGGGTAC+TGG | + | Chr5:10437267-10437286 | None:intergenic | 65.0% |
| !! | ACCCGTACAGGGTACTGCGC+GGG | + | Chr5:10437273-10437292 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 10437231 | 10438048 | 10437231 | ID=MsG0580024830.01;Name=MsG0580024830.01 |
| Chr5 | mRNA | 10437231 | 10438048 | 10437231 | ID=MsG0580024830.01.T01;Parent=MsG0580024830.01;Name=MsG0580024830.01.T01;_AED=0.59;_eAED=0.61;_QI=0|0|0|0.5|1|1|2|0|91 |
| Chr5 | exon | 10437989 | 10438048 | 10437989 | ID=MsG0580024830.01.T01:exon:1930;Parent=MsG0580024830.01.T01 |
| Chr5 | exon | 10437231 | 10437446 | 10437231 | ID=MsG0580024830.01.T01:exon:1929;Parent=MsG0580024830.01.T01 |
| Chr5 | CDS | 10437989 | 10438048 | 10437989 | ID=MsG0580024830.01.T01:cds;Parent=MsG0580024830.01.T01 |
| Chr5 | CDS | 10437231 | 10437446 | 10437231 | ID=MsG0580024830.01.T01:cds;Parent=MsG0580024830.01.T01 |
| Gene Sequence |
| Protein sequence |
