AlfalfaGEDB | MsG0580024843.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024843.01

KEGG: -
GO: -
PFAM: PF04784 PF14389

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024843.01.T01	XP_003611782.2	87.175	538	52	3	1	525	13	546	0	895

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024843.01.T01	G7K815	87.175	538	52	3	1	525	13	546	0.0	895

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048660.01	MsG0580024843.01	0.809044	2.298665e-50	1.335475e-47
MsG0180000489.01	MsG0580024843.01	0.831411	1.695122e-55	1.837917e-52
MsG0180001704.01	MsG0580024843.01	0.800696	1.276835e-48	5.984157e-46
MsG0180003499.01	MsG0580024843.01	0.807709	4.428300e-50	2.483864e-47
MsG0180004246.01	MsG0580024843.01	0.808744	2.663955e-50	1.535374e-47
MsG0180004899.01	MsG0580024843.01	0.800034	1.741504e-48	8.026090e-46
MsG0180004988.01	MsG0580024843.01	0.836302	1.013302e-56	1.271967e-53
MsG0180005051.01	MsG0580024843.01	0.823834	1.118677e-53	9.741225e-51
MsG0180005248.01	MsG0580024843.01	-0.808371	3.200522e-50	1.826840e-47
MsG0280011110.01	MsG0580024843.01	-0.814696	1.350750e-51	9.126687e-49
MsG0380012567.01	MsG0580024843.01	0.812113	4.991910e-51	3.146120e-48
MsG0380014386.01	MsG0580024843.01	0.815091	1.104090e-51	7.539759e-49
MsG0380014794.01	MsG0580024843.01	0.818364	2.036816e-52	1.521639e-49
MsG0380014909.01	MsG0580024843.01	0.802630	5.122276e-49	2.521414e-46
MsG0480018910.01	MsG0580024843.01	0.864262	1.379906e-64	4.305788e-61
MsG0480020229.01	MsG0580024843.01	0.828777	7.444002e-55	7.471891e-52
MsG0480021248.01	MsG0580024843.01	0.811476	6.870257e-51	4.257051e-48
MsG0480023190.01	MsG0580024843.01	0.807566	4.747444e-50	2.652963e-47
MsG0480023723.01	MsG0580024843.01	0.826293	2.936616e-54	2.742663e-51
MsG0480023877.01	MsG0580024843.01	0.809969	1.454580e-50	8.661118e-48
MsG0580024628.01	MsG0580024843.01	0.933076	3.253825e-95	2.260413e-90
MsG0580024759.01	MsG0580024843.01	0.801594	8.363552e-49	4.009747e-46
MsG0580024843.01	MsG0580025226.01	0.842587	2.361464e-58	3.597194e-55
MsG0580024843.01	MsG0580025827.01	0.842023	3.330740e-58	4.984378e-55
MsG0580024843.01	MsG0580026159.01	-0.826367	2.819758e-54	2.639183e-51
MsG0580024843.01	MsG0580026498.01	0.813641	2.309886e-51	1.516772e-48
MsG0580024843.01	MsG0580028428.01	0.818179	2.243472e-52	1.667380e-49
MsG0580024843.01	MsG0580028848.01	0.820621	6.226724e-53	4.951542e-50
MsG0580024843.01	MsG0580030071.01	0.824089	9.746992e-54	8.547967e-51
MsG0580024843.01	MsG0680030599.01	0.837781	4.244844e-57	5.573077e-54
MsG0580024843.01	MsG0680030680.01	0.817706	2.868643e-52	2.104549e-49
MsG0580024843.01	MsG0780036509.01	0.802715	4.917741e-49	2.426122e-46
MsG0580024843.01	MsG0780037351.01	0.807263	5.505586e-50	3.052678e-47
MsG0580024843.01	MsG0780040213.01	0.819131	1.364562e-52	1.041118e-49
MsG0580024843.01	MsG0780040235.01	0.818420	1.978309e-52	1.480224e-49
MsG0580024843.01	MsG0780040278.01	0.816367	5.736301e-52	4.056883e-49
MsG0580024843.01	MsG0880041864.01	0.827083	1.902896e-54	1.818193e-51
MsG0580024843.01	MsG0880042086.01	0.816283	5.989700e-52	4.226488e-49
MsG0580024843.01	MsG0880043298.01	0.822026	2.952775e-53	2.442419e-50
MsG0580024843.01	MsG0880044487.01	0.800007	1.763807e-48	8.123291e-46
MsG0580024843.01	MsG0880045402.01	0.813041	3.128160e-51	2.020873e-48
MsG0580024843.01	MsG0880045624.01	0.805188	1.505639e-49	7.914618e-47
MsG0580024843.01	MsG0880045668.01	0.808088	3.677597e-50	2.083516e-47
MsG0580024843.01	MsG0880046200.01	0.813779	2.153628e-51	1.419434e-48
MsG0580024843.01	MsG0880047394.01	0.803621	3.193650e-49	1.612462e-46
MsG0580024843.01	MsG0880047740.01	0.820332	7.253295e-53	5.722138e-50
MsG0580024843.01	MsG0880047758.01	0.800701	1.273817e-48	5.970722e-46
MsG0380015486.01	MsG0580024843.01	0.856432	3.230258e-62	7.709194e-59
MsG0380015784.01	MsG0580024843.01	0.802625	5.132415e-49	2.526125e-46
MsG0380017329.01	MsG0580024843.01	0.843088	1.737141e-58	2.687718e-55
MsG0380018037.01	MsG0580024843.01	0.807477	4.959485e-50	2.765163e-47
MsG0280006587.01	MsG0580024843.01	0.817618	3.004172e-52	2.198531e-49
MsG0280006996.01	MsG0580024843.01	0.811147	8.096793e-51	4.973588e-48
MsG0280007647.01	MsG0580024843.01	0.809520	1.817245e-50	1.069277e-47
MsG0280008165.01	MsG0580024843.01	0.801976	6.984269e-49	3.381008e-46
MsG0280008709.01	MsG0580024843.01	0.806027	1.003894e-49	5.392182e-47
MsG0280009169.01	MsG0580024843.01	0.812960	3.259444e-51	2.100986e-48
MsG0280009953.01	MsG0580024843.01	0.819646	1.041429e-52	8.060521e-50
MsG0280009954.01	MsG0580024843.01	0.807428	5.079495e-50	2.828508e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024843.01.T01	MTR_5g017790	87.175	538	52	3	1	525	13	546	0.0	895
MsG0580024843.01.T01	MTR_5g017790	87.175	538	52	3	1	525	1	534	0.0	894
MsG0580024843.01.T01	MTR_5g017790	95.349	344	16	0	1	344	13	356	0.0	618
MsG0580024843.01.T01	MTR_8g079840	61.460	589	156	11	1	525	16	597	0.0	598
MsG0580024843.01.T01	MTR_8g079840	58.163	490	134	11	1	426	16	498	4.04e-146	429
MsG0580024843.01.T01	MTR_3g101340	46.520	546	235	15	13	523	47	570	3.44e-131	394
MsG0580024843.01.T01	MTR_3g101340	46.617	532	247	14	13	523	22	537	7.77e-131	391
MsG0580024843.01.T01	MTR_3g101340	46.717	533	246	15	13	523	47	563	1.86e-130	391
MsG0580024843.01.T01	MTR_1g021040	48.162	544	223	15	21	522	18	544	5.16e-126	379
MsG0580024843.01.T01	MTR_4g058015	29.412	578	328	18	21	523	117	689	2.11e-58	206
MsG0580024843.01.T01	MTR_5g079910	28.597	549	292	14	21	523	15	509	4.34e-51	182
MsG0580024843.01.T01	MTR_8g078760	23.264	576	348	17	23	523	64	620	4.99e-31	127
MsG0580024843.01.T01	MTR_5g018820	22.581	589	370	11	1	523	50	618	1.90e-29	122
MsG0580024843.01.T01	MTR_5g012790	24.539	542	345	15	12	522	74	582	1.61e-26	114
MsG0580024843.01.T01	MTR_4g068920	24.085	519	315	13	71	523	123	628	4.35e-23	103
MsG0580024843.01.T01	MTR_5g079910	25.691	362	177	10	21	344	15	322	5.10e-15	77.0
MsG0580024843.01.T01	MTR_5g061340	26.761	213	138	6	287	486	738	945	8.44e-13	71.6
MsG0580024843.01.T01	MTR_3g073590	27.500	200	127	6	287	473	565	759	4.51e-12	69.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024843.01.T01	AT4G37080	53.484	574	191	13	16	525	49	610	1.14e-177	514
MsG0580024843.01.T01	AT4G37080	53.484	574	191	13	16	525	49	610	1.14e-177	514
MsG0580024843.01.T01	AT4G37080	53.484	574	191	13	16	525	36	597	1.70e-177	513
MsG0580024843.01.T01	AT4G37080	49.895	475	162	13	16	426	49	511	4.59e-117	355
MsG0580024843.01.T01	AT5G42690	44.847	524	233	14	20	522	22	510	8.09e-115	349
MsG0580024843.01.T01	AT5G42690	44.847	524	233	14	20	522	49	537	2.01e-114	349
MsG0580024843.01.T01	AT5G42690	44.847	524	233	14	20	522	49	537	2.01e-114	349
MsG0580024843.01.T01	AT5G42690	44.933	523	233	14	20	522	49	536	3.11e-114	349
MsG0580024843.01.T01	AT5G42690	44.615	520	232	14	24	522	1	485	3.10e-112	342
MsG0580024843.01.T01	AT2G39690	29.705	542	305	15	22	523	18	523	6.54e-53	187
MsG0580024843.01.T01	AT2G39690	29.755	531	297	15	33	523	3	497	8.45e-53	187
MsG0580024843.01.T01	AT2G39690	35.514	321	173	7	237	523	96	416	2.34e-52	184
MsG0580024843.01.T01	AT2G39690	29.575	541	306	15	22	523	18	522	2.48e-52	186
MsG0580024843.01.T01	AT2G39690	29.623	530	298	15	33	523	3	496	3.05e-52	185
MsG0580024843.01.T01	AT3G12540	28.942	501	269	15	71	523	42	503	2.98e-47	172
MsG0580024843.01.T01	AT3G12540	27.978	554	311	16	19	523	56	570	5.27e-47	172
MsG0580024843.01.T01	AT5G60720	28.000	575	290	19	66	523	122	689	5.85e-41	157
MsG0580024843.01.T01	AT5G47380	24.067	536	332	14	23	522	89	585	1.66e-31	129
MsG0580024843.01.T01	AT5G47380	24.067	536	332	14	23	522	33	529	2.39e-31	127
MsG0580024843.01.T01	AT5G47380	24.067	536	332	14	23	522	89	585	2.90e-31	128
MsG0580024843.01.T01	AT1G16750	33.969	262	145	6	283	522	271	526	2.64e-30	124
MsG0580024843.01.T01	AT2G23700	29.830	352	192	10	219	523	358	701	8.45e-29	121
MsG0580024843.01.T01	AT3G18900	29.430	316	181	11	233	523	227	525	2.10e-25	110
MsG0580024843.01.T01	AT3G18900	29.430	316	181	11	233	523	232	530	2.51e-25	110
MsG0580024843.01.T01	AT3G18900	29.430	316	181	11	233	523	151	449	5.87e-25	108
MsG0580024843.01.T01	AT3G18900	29.430	316	181	11	233	523	151	449	5.87e-25	108
MsG0580024843.01.T01	AT3G13000	24.694	571	313	16	23	522	20	544	8.74e-25	108
MsG0580024843.01.T01	AT3G13000	24.694	571	313	16	23	522	49	573	1.14e-24	108
MsG0580024843.01.T01	AT3G13000	24.694	571	313	16	23	522	49	573	1.14e-24	108
MsG0580024843.01.T01	AT5G66600	23.979	563	337	17	25	523	19	554	1.61e-23	104
MsG0580024843.01.T01	AT5G66600	23.801	563	338	17	25	523	73	608	1.84e-23	104
MsG0580024843.01.T01	AT5G66600	23.801	563	338	17	25	523	73	608	1.84e-23	104
MsG0580024843.01.T01	AT5G66600	23.801	563	338	17	25	523	73	608	1.84e-23	104
MsG0580024843.01.T01	AT5G66600	23.801	563	338	17	25	523	73	608	1.84e-23	104
MsG0580024843.01.T01	AT5G66600	23.801	563	338	17	25	523	73	608	1.84e-23	104
MsG0580024843.01.T01	AT5G66600	23.979	563	337	17	25	523	53	588	2.40e-23	104
MsG0580024843.01.T01	AT2G39690	27.088	443	249	14	22	426	18	424	6.43e-22	99.0
MsG0580024843.01.T01	AT2G39690	26.923	442	250	14	22	426	18	423	1.75e-21	97.4
MsG0580024843.01.T01	AT1G76620	25.000	336	191	9	237	523	193	516	5.11e-21	96.7
MsG0580024843.01.T01	AT1G43020	26.370	292	171	10	236	511	151	414	1.39e-20	94.7
MsG0580024843.01.T01	AT1G21060	26.498	317	195	9	237	525	187	493	1.28e-18	89.4
MsG0580024843.01.T01	AT1G21060	26.498	317	195	9	237	525	199	505	1.33e-18	89.4
MsG0580024843.01.T01	AT5G47380	22.851	442	267	13	23	429	89	491	1.64e-15	79.7
MsG0580024843.01.T01	AT4G08550	26.337	243	158	8	289	519	402	635	4.98e-15	78.6
MsG0580024843.01.T01	AT4G08550	27.027	222	146	7	289	498	456	673	6.23e-15	78.2
MsG0580024843.01.T01	AT5G47380	22.831	438	264	13	23	425	89	487	9.99e-15	77.0
MsG0580024843.01.T01	AT2G39690	26.239	343	186	12	33	344	3	309	6.53e-14	73.9
MsG0580024843.01.T01	AT5G66600	23.176	466	270	15	25	426	73	514	8.78e-11	64.7
MsG0580024843.01.T01	AT5G66600	23.176	466	270	15	25	426	73	514	8.78e-11	64.7
MsG0580024843.01.T01	AT5G66600	23.176	466	270	15	25	426	73	514	8.78e-11	64.7
MsG0580024843.01.T01	AT5G66600	23.176	466	270	15	25	426	73	514	8.78e-11	64.7

Find 112 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTAGTAGAAAGTTAGTAAA+TGG	0.182205	5:+10641805	MsG0580024843.01.T01:CDS
TCCTAGATTGTCCTTGAATA+TGG	0.203016	5:-10642200	None:intergenic
ACCAGGAGAAACTTGCATTT+TGG	0.212496	5:+10642504	MsG0580024843.01.T01:CDS
TGCCCTATGATTTCAGTTTC+AGG	0.214439	5:+10644179	MsG0580024843.01.T01:CDS
ATGAAAGCAAGAGGCATATT+TGG	0.216429	5:+10643222	MsG0580024843.01.T01:CDS
TGGATCCTTTGATGGAATTC+TGG	0.239508	5:-10641699	None:intergenic
ATATCCTCTGAAACTCTATT+TGG	0.284459	5:-10642142	None:intergenic
TTTAAGATTATTGGTTATAA+TGG	0.288762	5:-10644542	None:intergenic
CTTGGAATCTTTGTTGGATT+GGG	0.291306	5:+10644063	MsG0580024843.01.T01:CDS
CAAGTAATGTTTCCTTTGAT+AGG	0.299053	5:+10644347	MsG0580024843.01.T01:three_prime_UTR
CTCTTCTGGTATTGATGATA+AGG	0.304077	5:-10641854	None:intergenic
GAAATCTATTGAAAAGAAAA+TGG	0.326415	5:+10641876	MsG0580024843.01.T01:CDS
CTTGTCAGCTGATTCTTGTT+TGG	0.329258	5:-10641932	None:intergenic
AGCAAGAGGCATATTTGGTT+TGG	0.336129	5:+10643227	MsG0580024843.01.T01:CDS
GACCTTTGTAAACTGCAATA+AGG	0.339529	5:+10644375	MsG0580024843.01.T01:three_prime_UTR
AGAGCACTCACTAGACCTTT+AGG	0.357274	5:+10641330	MsG0580024843.01.T01:CDS
TATGAGAATTTCCATATTCA+AGG	0.365612	5:+10642189	MsG0580024843.01.T01:CDS
AGCAAAGAGAGATTATTTAC+AGG	0.366908	5:+10643961	MsG0580024843.01.T01:CDS
TTGATGTTTGGATCCTTTGA+TGG	0.368156	5:-10641707	None:intergenic
TTTCCGACAAGGTTTATATC+AGG	0.369828	5:+10641621	MsG0580024843.01.T01:CDS
AAAAGGAGGGAGCAGGAAAA+TGG	0.374679	5:+10641101	MsG0580024843.01.T01:CDS
ACCTCTCACTGGATTATTTG+TGG	0.376104	5:-10644239	None:intergenic
GCTGCCTTCCCCTTCTTTCA+AGG	0.376152	5:-10644132	None:intergenic
CAACCTGAAACTGAAATCAT+AGG	0.378498	5:-10644182	None:intergenic
ATATCGAAAACTAGAGATAT+TGG	0.379801	5:+10642265	MsG0580024843.01.T01:CDS
AAGCAGGAAAAGATGGAAAA+AGG	0.383928	5:+10641084	MsG0580024843.01.T01:exon
ACTTGGAATCTTTGTTGGAT+TGG	0.390859	5:+10644062	MsG0580024843.01.T01:CDS
CGTGGCTGAAGTTGCGGTAT+TGG	0.393024	5:+10641561	MsG0580024843.01.T01:CDS
TTTGGAATGGTCTGAACCTT+TGG	0.394809	5:+10643245	MsG0580024843.01.T01:CDS
CTTTCTACTAGAAACACTTC+TGG	0.401440	5:-10641794	None:intergenic
TTGGTTGGTGATTTCTTCAC+TGG	0.405802	5:-10641913	None:intergenic
AAATGAGAAAGAAAGATAGA+AGG	0.406943	5:-10641010	None:intergenic
TCCATATTCAAGGACAATCT+AGG	0.414001	5:+10642199	MsG0580024843.01.T01:CDS
AAAGGACTTGGAATCTTTGT+TGG	0.415429	5:+10644057	MsG0580024843.01.T01:CDS
TCAGCTGATTCTTGTTTGGT+TGG	0.420192	5:-10641928	None:intergenic
AGGGCGTCGAGAAAGGAAAA+TGG	0.421258	5:+10641146	MsG0580024843.01.T01:CDS
GATATTGAACAGATACCATA+TGG	0.422044	5:-10642247	None:intergenic
CTAGTAGAAAGTTAGTAAAT+GGG	0.423941	5:+10641806	MsG0580024843.01.T01:CDS
GCTTCCTTTCTCAGTTCAAC+AGG	0.438309	5:-10644100	None:intergenic
TGCAGATCACGTGAAGTTGC+AGG	0.442494	5:+10641957	MsG0580024843.01.T01:CDS
CTTACATAATTCAAGTGATA+AGG	0.444130	5:-10643052	None:intergenic
TCCAAAATGCAAGTTTCTCC+TGG	0.448612	5:-10642505	None:intergenic
ACAAAATTCATCAGACGATA+AGG	0.452744	5:+10642099	MsG0580024843.01.T01:CDS
ATAAGGTGTTAGAAGTTGAT+AGG	0.456293	5:+10642116	MsG0580024843.01.T01:CDS
AAGTAATGTTTCCTTTGATA+GGG	0.461447	5:+10644348	MsG0580024843.01.T01:three_prime_UTR
TGCAGGTTGATAAGTTAAAG+AGG	0.463381	5:+10641268	MsG0580024843.01.T01:intron
ACTTGCCTCTGTGAACTTGA+AGG	0.469270	5:+10642472	MsG0580024843.01.T01:CDS
CAGTTGAATGCCTTGAAAGA+AGG	0.473839	5:+10644122	MsG0580024843.01.T01:CDS
TTGCACTATCTTGTGGAAGC+TGG	0.481719	5:+10643274	MsG0580024843.01.T01:CDS
TCTCACTTAATGTGCAAAGC+AGG	0.484583	5:+10641068	MsG0580024843.01.T01:five_prime_UTR
ATGTGCAAAGCAGGAAAAGA+TGG	0.484944	5:+10641077	MsG0580024843.01.T01:exon
ACTCGACTTTGCAAAGGACT+TGG	0.489476	5:+10644045	MsG0580024843.01.T01:CDS
CTTCCTGATATAAACCTTGT+CGG	0.493206	5:-10641624	None:intergenic
TTGATGGAATTCTGGTCCAT+CGG	0.493905	5:-10641691	None:intergenic
CTTACATGGGAAGGGAGATA+AGG	0.496981	5:-10641372	None:intergenic
TGATGGAATTCTGGTCCATC+GGG	0.498652	5:-10641690	None:intergenic
GGCTGAAGTTGCGGTATTGG+AGG	0.504477	5:+10641564	MsG0580024843.01.T01:CDS
ACATTAACAGCACCTCTCAC+TGG	0.505947	5:-10644250	None:intergenic
GAGAAAGAAAGATAGAAGGA+AGG	0.505999	5:-10641006	None:intergenic
GTTACATTTGCACTATCTTG+TGG	0.506373	5:+10643267	MsG0580024843.01.T01:CDS
AGTTGAATGCCTTGAAAGAA+GGG	0.509505	5:+10644123	MsG0580024843.01.T01:CDS
ACACCTCCACGTGATCCATA+TGG	0.511970	5:+10642232	MsG0580024843.01.T01:CDS
ATATGTACCACGTGTGAAAG+AGG	0.517572	5:-10644154	None:intergenic
GAGGCATATTTGGTTTGGAA+TGG	0.523893	5:+10643232	MsG0580024843.01.T01:CDS
GGGAGATAAGGAGGAAGACG+AGG	0.525815	5:-10641360	None:intergenic
GGTAGTTCGATTAGAAGAGA+AGG	0.529827	5:+10641591	MsG0580024843.01.T01:CDS
GCTACCTGTTGAACTGAGAA+AGG	0.530299	5:+10644096	MsG0580024843.01.T01:CDS
TTTCATTCGGTTGAAATCAA+CGG	0.530335	5:-10642323	None:intergenic
CTTAATCTACTTACATGGGA+AGG	0.531642	5:-10641381	None:intergenic
GATTATTTACAGGCAGCAGT+TGG	0.534652	5:+10643971	MsG0580024843.01.T01:CDS
TTGCTTGTACACCAGTAACC+TGG	0.536984	5:+10644202	MsG0580024843.01.T01:exon
TAATTCCAAAGATACTTGAC+TGG	0.540269	5:+10644017	MsG0580024843.01.T01:CDS
CTTGAAGGGTCTTACTCACC+AGG	0.555043	5:+10642487	MsG0580024843.01.T01:CDS
TCGGTTGAAATCAACGGTGG+AGG	0.556026	5:-10642317	None:intergenic
TGGAGATGATAAGTAAAGAC+TGG	0.558108	5:+10640912	None:intergenic
TGGTGATTTCTTCACTGGAG+TGG	0.560430	5:-10641908	None:intergenic
AACCTGAAACTGAAATCATA+GGG	0.561621	5:-10644181	None:intergenic
TGACAGGCCACAATAGTAGT+GGG	0.565218	5:+10642980	MsG0580024843.01.T01:intron
GACCTTATTGCAGTTTACAA+AGG	0.565949	5:-10644377	None:intergenic
AGTTGCGGTATTGGAGGAAG+AGG	0.573287	5:+10641570	MsG0580024843.01.T01:CDS
AGACGAGGAAGTGCTCCTAA+AGG	0.579607	5:-10641345	None:intergenic
AGAAAATTCGATTGACCCGA+TGG	0.580147	5:+10641675	MsG0580024843.01.T01:CDS
TCAGTTCAACAGGTAGCTGA+AGG	0.581607	5:-10644090	None:intergenic
CTTGCCTCTGTGAACTTGAA+GGG	0.587168	5:+10642473	MsG0580024843.01.T01:CDS
AGAGCTCGTGGCTGAAGTTG+CGG	0.588350	5:+10641555	MsG0580024843.01.T01:CDS
AAAGGTCAAAACCCTATCAA+AGG	0.588642	5:-10644359	None:intergenic
GTGACAGGCCACAATAGTAG+TGG	0.594519	5:+10642979	MsG0580024843.01.T01:intron
AATGATGAGATGAAAGCAAG+AGG	0.596232	5:+10643213	MsG0580024843.01.T01:CDS
TTAATCTACTTACATGGGAA+GGG	0.597323	5:-10641380	None:intergenic
CAGATACCATATGGATCACG+TGG	0.601566	5:-10642238	None:intergenic
GACACCAAATAGAGTTTCAG+AGG	0.605334	5:+10642138	MsG0580024843.01.T01:CDS
CTGTCTACATTTCCTCCAAG+AGG	0.607010	5:+10641647	MsG0580024843.01.T01:CDS
ATGGACCAGAATTCCATCAA+AGG	0.609091	5:+10641694	MsG0580024843.01.T01:CDS
TCAAAGGATCCAAACATCAA+AGG	0.609297	5:+10641710	MsG0580024843.01.T01:CDS
ATGCTTACTGCTAAGTGCGA+AGG	0.612958	5:-10644430	None:intergenic
GATAGTGCAAATGTAACCAA+AGG	0.614690	5:-10643261	None:intergenic
CATTCGGTTGAAATCAACGG+TGG	0.616978	5:-10642320	None:intergenic
ACAGGCCACAATAGTAGTGG+GGG	0.633577	5:+10642982	MsG0580024843.01.T01:intron
TGGAAGCTGGTCATCACCCG+TGG	0.637861	5:+10643287	MsG0580024843.01.T01:CDS
ACATGGGAAGGGAGATAAGG+AGG	0.643159	5:-10641369	None:intergenic
GACAGGCCACAATAGTAGTG+GGG	0.662710	5:+10642981	MsG0580024843.01.T01:intron
ACCACAAATAATCCAGTGAG+AGG	0.668723	5:+10644238	MsG0580024843.01.T01:three_prime_UTR
GTTGAATGCCTTGAAAGAAG+GGG	0.670351	5:+10644124	MsG0580024843.01.T01:CDS
AGCTTAATTCTACTACCACT+AGG	0.672932	5:+10641755	MsG0580024843.01.T01:CDS
AAGAGAAATCTGAGGCCTAG+TGG	0.675130	5:-10641770	None:intergenic
AATGCCTTGAAAGAAGGGGA+AGG	0.676784	5:+10644128	MsG0580024843.01.T01:CDS
AAGGTTGTGAATTTCCGACA+AGG	0.684209	5:+10641610	MsG0580024843.01.T01:CDS
AAGGCAGCCTCTTTCACACG+TGG	0.731282	5:+10644147	MsG0580024843.01.T01:CDS
CAGGCCACAATAGTAGTGGG+GGG	0.734448	5:+10642983	MsG0580024843.01.T01:intron
ATACCATATGGATCACGTGG+AGG	0.760579	5:-10642235	None:intergenic
CTTCTGGCAAGAGAAATCTG+AGG	0.762148	5:-10641778	None:intergenic
AAGACCCTTCAAGTTCACAG+AGG	0.790606	5:-10642477	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATACAAAAACAAAA+AGG	+	Chr5:10644507-10644526	MsG0580024843.01.T01:three_prime_UTR	10.0%
!!	AAATAATACAAAAACAAAAA+GGG	+	Chr5:10644508-10644527	MsG0580024843.01.T01:three_prime_UTR	10.0%
!!	ATAAATCAATGTATTTATTA+AGG	+	Chr5:10643848-10643867	MsG0580024843.01.T01:intron	10.0%
!!	AATAATACAAAAACAAAAAG+GGG	+	Chr5:10644509-10644528	MsG0580024843.01.T01:three_prime_UTR	15.0%
!!	AATGCTTATCTAAATATAAT+TGG	-	Chr5:10642932-10642951	None:intergenic	15.0%
!!!	ACATTAACTATTTTCTTTTT+CGG	+	Chr5:10643351-10643370	MsG0580024843.01.T01:intron	15.0%
!!!	ATATATGAAAATTTGTTTCA+GGG	+	Chr5:10643434-10643453	MsG0580024843.01.T01:intron	15.0%
!!!	ATCTATCATAAATTTGTTAT+CGG	+	Chr5:10643634-10643653	MsG0580024843.01.T01:intron	15.0%
!!!	ATTTTATTTTATGAAGGTAA+GGG	+	Chr5:10643901-10643920	MsG0580024843.01.T01:intron	15.0%
!!!	TATATATGAAAATTTGTTTC+AGG	+	Chr5:10643433-10643452	MsG0580024843.01.T01:intron	15.0%
!!!	TATTTTATTTTATGAAGGTA+AGG	+	Chr5:10643900-10643919	MsG0580024843.01.T01:intron	15.0%
!!	AAAATTAGAAAAGTGGAAAT+TGG	-	Chr5:10643779-10643798	None:intergenic	20.0%
!!	ACAGTCTGTAAAAAAAAAAA+GGG	-	Chr5:10644402-10644421	None:intergenic	20.0%
!!	ATAATACAAAAACAAAAAGG+GGG	+	Chr5:10644510-10644529	MsG0580024843.01.T01:three_prime_UTR	20.0%
!!	CGATTAAAGTAAGTTATAAA+GGG	+	Chr5:10642367-10642386	MsG0580024843.01.T01:intron	20.0%
!!	GAAATCTATTGAAAAGAAAA+TGG	+	Chr5:10641876-10641895	MsG0580024843.01.T01:CDS	20.0%
!!	TACAGTCTGTAAAAAAAAAA+AGG	-	Chr5:10644403-10644422	None:intergenic	20.0%
!!	TCGATTAAAGTAAGTTATAA+AGG	+	Chr5:10642366-10642385	MsG0580024843.01.T01:intron	20.0%
!!!	AATGTATTTATTAAGGCATT+TGG	+	Chr5:10643855-10643874	MsG0580024843.01.T01:intron	20.0%
!!!	ATTTTCATATATACTAGTCA+AGG	-	Chr5:10643426-10643445	None:intergenic	20.0%
!!!	CACATAATACTTTGAAAAAA+GGG	+	Chr5:10641127-10641146	MsG0580024843.01.T01:CDS	20.0%
!!!	TCTATCATAAATTTGTTATC+GGG	+	Chr5:10643635-10643654	MsG0580024843.01.T01:intron	20.0%
!	AAATGAGAAAGAAAGATAGA+AGG	-	Chr5:10641013-10641032	None:intergenic	25.0%
!	AAGTAATGTTTCCTTTGATA+GGG	+	Chr5:10644348-10644367	MsG0580024843.01.T01:three_prime_UTR	25.0%
!	AATCTCACAAAAACTATTGT+AGG	-	Chr5:10642293-10642312	None:intergenic	25.0%
!	ATAGTTATCATTCTTGATGT+GGG	+	Chr5:10643818-10643837	MsG0580024843.01.T01:intron	25.0%
!	ATATCGAAAACTAGAGATAT+TGG	+	Chr5:10642265-10642284	MsG0580024843.01.T01:CDS	25.0%
!	CTAGTAGAAAGTTAGTAAAT+GGG	+	Chr5:10641806-10641825	MsG0580024843.01.T01:CDS	25.0%
!	CTTACATAATTCAAGTGATA+AGG	-	Chr5:10643055-10643074	None:intergenic	25.0%
!	GCACATCATATAGTAATTAT+TGG	-	Chr5:10641996-10642015	None:intergenic	25.0%
!	GTAGAACAAATAATAATGCA+AGG	-	Chr5:10641202-10641221	None:intergenic	25.0%
!	TAGATGAAAATATTGTTCTG+TGG	+	Chr5:10643328-10643347	MsG0580024843.01.T01:intron	25.0%
!	TATGAGAATTTCCATATTCA+AGG	+	Chr5:10642189-10642208	MsG0580024843.01.T01:CDS	25.0%
!	TCTAGTAGAAAGTTAGTAAA+TGG	+	Chr5:10641805-10641824	MsG0580024843.01.T01:CDS	25.0%
!!	ATTTTTGAACTTTGATGTTC+TGG	+	Chr5:10644286-10644305	MsG0580024843.01.T01:three_prime_UTR	25.0%
!!	GAAACACTGAATTTTTCATT+CGG	-	Chr5:10642339-10642358	None:intergenic	25.0%
!!	GCACATAATACTTTGAAAAA+AGG	+	Chr5:10641126-10641145	MsG0580024843.01.T01:CDS	25.0%
!!	TTTTCAATAGATTTCTCTTC+TGG	-	Chr5:10641871-10641890	None:intergenic	25.0%
!!!	ATGTTCTGGATTTTGTATAT+TGG	+	Chr5:10644300-10644319	MsG0580024843.01.T01:three_prime_UTR	25.0%
!!!	CTTTTTGTTGATTCTACATA+TGG	+	Chr5:10642397-10642416	MsG0580024843.01.T01:intron	25.0%
!!!	TTTTTTTACAGACTGTAATG+AGG	+	Chr5:10644406-10644425	MsG0580024843.01.T01:three_prime_UTR	25.0%
!!!	TTTTTTTTTGATTGATCAGC+TGG	+	Chr5:10642048-10642067	MsG0580024843.01.T01:intron	25.0%
	AAAAACCAGTCAAGTATCTT+TGG	-	Chr5:10644025-10644044	None:intergenic	30.0%
	AACCTGAAACTGAAATCATA+GGG	-	Chr5:10644184-10644203	None:intergenic	30.0%
	AAGTTTCCCAAATAAAAACC+TGG	-	Chr5:10642456-10642475	None:intergenic	30.0%
	ACAAAATTCATCAGACGATA+AGG	+	Chr5:10642099-10642118	MsG0580024843.01.T01:CDS	30.0%
	AGAAATCCATTCATGTCAAT+AGG	-	Chr5:10642888-10642907	None:intergenic	30.0%
	AGCAAAGAGAGATTATTTAC+AGG	+	Chr5:10643961-10643980	MsG0580024843.01.T01:CDS	30.0%
	ATTGAGAAACAAACATACCA+CGG	-	Chr5:10643307-10643326	None:intergenic	30.0%
	CAAGTAATGTTTCCTTTGAT+AGG	+	Chr5:10644347-10644366	MsG0580024843.01.T01:three_prime_UTR	30.0%
	CATAGTTATCATTCTTGATG+TGG	+	Chr5:10643817-10643836	MsG0580024843.01.T01:intron	30.0%
	CTGACTTAATCTACTTACAT+GGG	-	Chr5:10641388-10641407	None:intergenic	30.0%
	GATATTGAACAGATACCATA+TGG	-	Chr5:10642250-10642269	None:intergenic	30.0%
	GATGATCACATAAGACATTA+AGG	-	Chr5:10643382-10643401	None:intergenic	30.0%
	GCTAGTGAAAATTAGAAAAG+TGG	-	Chr5:10643786-10643805	None:intergenic	30.0%
	GCTGAGAATTGAAATAATCT+AGG	+	Chr5:10643476-10643495	MsG0580024843.01.T01:intron	30.0%
	GTAATCTACAAAGCATAACA+AGG	-	Chr5:10643133-10643152	None:intergenic	30.0%
	GTATCTTACATTCATCATAC+AGG	-	Chr5:10642546-10642565	None:intergenic	30.0%
	TAATTCCAAAGATACTTGAC+TGG	+	Chr5:10644017-10644036	MsG0580024843.01.T01:CDS	30.0%
	TTAATCTACTTACATGGGAA+GGG	-	Chr5:10641383-10641402	None:intergenic	30.0%
	TTGAGGTGACTACAATTATT+TGG	+	Chr5:10642682-10642701	MsG0580024843.01.T01:intron	30.0%
	TTTCATTCGGTTGAAATCAA+CGG	-	Chr5:10642326-10642345	None:intergenic	30.0%
!	ACTGACTTAATCTACTTACA+TGG	-	Chr5:10641389-10641408	None:intergenic	30.0%
!	ATAAGGTGTTAGAAGTTGAT+AGG	+	Chr5:10642116-10642135	MsG0580024843.01.T01:CDS	30.0%
!	ATTGACATGAATGGATTTCT+TGG	+	Chr5:10642888-10642907	MsG0580024843.01.T01:intron	30.0%
!	CAAGTTACAAGTCAGTTTTT+AGG	-	Chr5:10643570-10643589	None:intergenic	30.0%
!	CTAAAAGAGTTGTCTTTTTG+AGG	+	Chr5:10642608-10642627	MsG0580024843.01.T01:intron	30.0%
!	CTTTTCTAATTTTCACTAGC+AGG	+	Chr5:10643786-10643805	MsG0580024843.01.T01:intron	30.0%
!	GCAGTAAGCATAGATTTTAT+AGG	+	Chr5:10644442-10644461	MsG0580024843.01.T01:three_prime_UTR	30.0%
!	TTGTAGTCATATTTAGAGCA+TGG	+	Chr5:10642710-10642729	MsG0580024843.01.T01:intron	30.0%
!!	AAACTGACTTGTAACTTGTA+AGG	+	Chr5:10643572-10643591	MsG0580024843.01.T01:intron	30.0%
!!	ACATGTCCAGGTTTTTATTT+GGG	+	Chr5:10642447-10642466	MsG0580024843.01.T01:intron	30.0%
!!	ATATCCTCTGAAACTCTATT+TGG	-	Chr5:10642145-10642164	None:intergenic	30.0%
!!	TGATTTTGACCTTTGATGTT+TGG	-	Chr5:10641722-10641741	None:intergenic	30.0%
!!!	ATTTTTTGCAGCTTTTAGAC+AGG	-	Chr5:10643196-10643215	None:intergenic	30.0%
!!!	TCATATTTTGATGCGATCAT+GGG	-	Chr5:10644476-10644495	None:intergenic	30.0%
	AAAGGACTTGGAATCTTTGT+TGG	+	Chr5:10644057-10644076	MsG0580024843.01.T01:CDS	35.0%
	AAAGGTCAAAACCCTATCAA+AGG	-	Chr5:10644362-10644381	None:intergenic	35.0%
	AAGCAGGAAAAGATGGAAAA+AGG	+	Chr5:10641084-10641103	MsG0580024843.01.T01:exon	35.0%
	AATGATGAGATGAAAGCAAG+AGG	+	Chr5:10643213-10643232	MsG0580024843.01.T01:CDS	35.0%
	ACAAAAGCTGAGAACATTAG+AGG	-	Chr5:10642790-10642809	None:intergenic	35.0%
	ACTTGGAATCTTTGTTGGAT+TGG	+	Chr5:10644062-10644081	MsG0580024843.01.T01:CDS	35.0%
	AGCTTAATTCTACTACCACT+AGG	+	Chr5:10641755-10641774	MsG0580024843.01.T01:CDS	35.0%
	AGGAAAAGATGGAAAAAGGA+GGG	+	Chr5:10641088-10641107	MsG0580024843.01.T01:exon	35.0%
	AGTTGAATGCCTTGAAAGAA+GGG	+	Chr5:10644123-10644142	MsG0580024843.01.T01:CDS	35.0%
	ATTCACTACTTGTCACTACA+AGG	+	Chr5:10642580-10642599	MsG0580024843.01.T01:intron	35.0%
	CAAAAGCTGAGAACATTAGA+GGG	-	Chr5:10642789-10642808	None:intergenic	35.0%
	CAACCTGAAACTGAAATCAT+AGG	-	Chr5:10644185-10644204	None:intergenic	35.0%
	CCTATGATACCAAACAAAAC+CGG	-	Chr5:10643712-10643731	None:intergenic	35.0%
	CTAATCTAAAAGACATGTCC+AGG	+	Chr5:10642435-10642454	MsG0580024843.01.T01:intron	35.0%
	CTTAATCTACTTACATGGGA+AGG	-	Chr5:10641384-10641403	None:intergenic	35.0%
	CTTCCTGATATAAACCTTGT+CGG	-	Chr5:10641627-10641646	None:intergenic	35.0%
	CTTGGAATCTTTGTTGGATT+GGG	+	Chr5:10644063-10644082	MsG0580024843.01.T01:CDS	35.0%
	CTTTCTACTAGAAACACTTC+TGG	-	Chr5:10641797-10641816	None:intergenic	35.0%
	GAAGCAAAGATGAATTGAGA+AGG	-	Chr5:10642860-10642879	None:intergenic	35.0%
	GACCTTATTGCAGTTTACAA+AGG	-	Chr5:10644380-10644399	None:intergenic	35.0%
	GACCTTTGTAAACTGCAATA+AGG	+	Chr5:10644375-10644394	MsG0580024843.01.T01:three_prime_UTR	35.0%
	GAGAAAGAAAGATAGAAGGA+AGG	-	Chr5:10641009-10641028	None:intergenic	35.0%
	GATAGTGCAAATGTAACCAA+AGG	-	Chr5:10643264-10643283	None:intergenic	35.0%
	GTTACATTTGCACTATCTTG+TGG	+	Chr5:10643267-10643286	MsG0580024843.01.T01:CDS	35.0%
	TACAAAAACAAAAAGGGGGT+TGG	+	Chr5:10644514-10644533	MsG0580024843.01.T01:three_prime_UTR	35.0%
	TCAAAGGATCCAAACATCAA+AGG	+	Chr5:10641710-10641729	MsG0580024843.01.T01:CDS	35.0%
	TCCAAAAAAAACCAGGTTAC+TGG	-	Chr5:10644216-10644235	None:intergenic	35.0%
	TCCATATTCAAGGACAATCT+AGG	+	Chr5:10642199-10642218	MsG0580024843.01.T01:CDS	35.0%
	TCCTAGATTGTCCTTGAATA+TGG	-	Chr5:10642203-10642222	None:intergenic	35.0%
	TGTAGTCACCTCAACATATT+AGG	-	Chr5:10642676-10642695	None:intergenic	35.0%
	TTACCTTGCCTAATATGTTG+AGG	+	Chr5:10642665-10642684	MsG0580024843.01.T01:intron	35.0%
	TTAGGTCATATCTCAAGTGA+AGG	-	Chr5:10643552-10643571	None:intergenic	35.0%
	TTGACCAGTCTTTACTTATC+AGG	-	Chr5:10640919-10640938	None:intergenic	35.0%
	TTGAGAAACAAACATACCAC+GGG	-	Chr5:10643306-10643325	None:intergenic	35.0%
	TTGATGTTTGGATCCTTTGA+TGG	-	Chr5:10641710-10641729	None:intergenic	35.0%
	TTTCCGACAAGGTTTATATC+AGG	+	Chr5:10641621-10641640	MsG0580024843.01.T01:CDS	35.0%
!	AAGGCATTTGGTTGAATTGA+TGG	+	Chr5:10643867-10643886	MsG0580024843.01.T01:intron	35.0%
!	ATGAAAGCAAGAGGCATATT+TGG	+	Chr5:10643222-10643241	MsG0580024843.01.T01:CDS	35.0%
!	ATTTTAGATGTTAGAGCTCG+TGG	+	Chr5:10641543-10641562	MsG0580024843.01.T01:intron	35.0%
!	GACATGTCCAGGTTTTTATT+TGG	+	Chr5:10642446-10642465	MsG0580024843.01.T01:intron	35.0%
!	GTTTTTACTCGACTTTGCAA+AGG	+	Chr5:10644039-10644058	MsG0580024843.01.T01:CDS	35.0%
!	TGTTTCAGGGTGTTATGTAA+TGG	+	Chr5:10643447-10643466	MsG0580024843.01.T01:intron	35.0%
!!	AACTTGGTAATGTTTGTGAC+AGG	+	Chr5:10642964-10642983	MsG0580024843.01.T01:intron	35.0%
!!	CATTCGAGTTGACTAAAACT+TGG	+	Chr5:10642948-10642967	MsG0580024843.01.T01:intron	35.0%
!!	CTCTTCTGGTATTGATGATA+AGG	-	Chr5:10641857-10641876	None:intergenic	35.0%
!!	TGCAGGTTGATAAGTTAAAG+AGG	+	Chr5:10641268-10641287	MsG0580024843.01.T01:intron	35.0%
!!!	ACCAGTAACCTGGTTTTTTT+TGG	+	Chr5:10644212-10644231	MsG0580024843.01.T01:exon	35.0%
!!!	CTCATATTTTGATGCGATCA+TGG	-	Chr5:10644477-10644496	None:intergenic	35.0%
!!!	CTTATTTTGATCCTACAAGC+CGG	+	Chr5:10643690-10643709	MsG0580024843.01.T01:intron	35.0%
	AAGGTTGTGAATTTCCGACA+AGG	+	Chr5:10641610-10641629	MsG0580024843.01.T01:CDS	40.0%
	ACCACAAATAATCCAGTGAG+AGG	+	Chr5:10644238-10644257	MsG0580024843.01.T01:three_prime_UTR	40.0%
	ACCTCTCACTGGATTATTTG+TGG	-	Chr5:10644242-10644261	None:intergenic	40.0%
	AGAAAATTCGATTGACCCGA+TGG	+	Chr5:10641675-10641694	MsG0580024843.01.T01:CDS	40.0%
	ATATGTACCACGTGTGAAAG+AGG	-	Chr5:10644157-10644176	None:intergenic	40.0%
	ATCAGTGCTACAACCATTTC+AGG	-	Chr5:10642749-10642768	None:intergenic	40.0%
	ATGGACCAGAATTCCATCAA+AGG	+	Chr5:10641694-10641713	MsG0580024843.01.T01:CDS	40.0%
	ATGTGCAAAGCAGGAAAAGA+TGG	+	Chr5:10641077-10641096	MsG0580024843.01.T01:exon	40.0%
	ATTATCCAGCAAGGTACAGT+AGG	+	Chr5:10643666-10643685	MsG0580024843.01.T01:intron	40.0%
	CAGGAAAAGATGGAAAAAGG+AGG	+	Chr5:10641087-10641106	MsG0580024843.01.T01:exon	40.0%
	CAGTTGAATGCCTTGAAAGA+AGG	+	Chr5:10644122-10644141	MsG0580024843.01.T01:CDS	40.0%
	CCATCACCTATTGACATGAA+TGG	+	Chr5:10642879-10642898	MsG0580024843.01.T01:intron	40.0%
	CCATTCATGTCAATAGGTGA+TGG	-	Chr5:10642882-10642901	None:intergenic	40.0%
	GACACCAAATAGAGTTTCAG+AGG	+	Chr5:10642138-10642157	MsG0580024843.01.T01:CDS	40.0%
	GATTATTTACAGGCAGCAGT+TGG	+	Chr5:10643971-10643990	MsG0580024843.01.T01:CDS	40.0%
	GGTAGTTCGATTAGAAGAGA+AGG	+	Chr5:10641591-10641610	MsG0580024843.01.T01:CDS	40.0%
	GTGGTTGTCCAAAAAAAACC+AGG	-	Chr5:10644223-10644242	None:intergenic	40.0%
	GTTGAATGCCTTGAAAGAAG+GGG	+	Chr5:10644124-10644143	MsG0580024843.01.T01:CDS	40.0%
	TATGATGTGCGAAACGATTG+AGG	+	Chr5:10642006-10642025	MsG0580024843.01.T01:intron	40.0%
	TCACCTCAACATATTAGGCA+AGG	-	Chr5:10642671-10642690	None:intergenic	40.0%
	TCCAAAATGCAAGTTTCTCC+TGG	-	Chr5:10642508-10642527	None:intergenic	40.0%
	TCTCAAGTGAAGGTGAGATT+TGG	-	Chr5:10643542-10643561	None:intergenic	40.0%
	TCTCACTTAATGTGCAAAGC+AGG	+	Chr5:10641068-10641087	MsG0580024843.01.T01:five_prime_UTR	40.0%
	TGCCCTATGATTTCAGTTTC+AGG	+	Chr5:10644179-10644198	MsG0580024843.01.T01:CDS	40.0%
	TGGATCCTTTGATGGAATTC+TGG	-	Chr5:10641702-10641721	None:intergenic	40.0%
	TTATCCAGCAAGGTACAGTA+GGG	+	Chr5:10643667-10643686	MsG0580024843.01.T01:intron	40.0%
	TTGATGGAATTCTGGTCCAT+CGG	-	Chr5:10641694-10641713	None:intergenic	40.0%
!	ACCAGGAGAAACTTGCATTT+TGG	+	Chr5:10642504-10642523	MsG0580024843.01.T01:CDS	40.0%
!	AGCAAGAGGCATATTTGGTT+TGG	+	Chr5:10643227-10643246	MsG0580024843.01.T01:CDS	40.0%
!	ATTTTCTGCATTCCTCTTGG+AGG	-	Chr5:10641662-10641681	None:intergenic	40.0%
!	CGAATTTTCTGCATTCCTCT+TGG	-	Chr5:10641665-10641684	None:intergenic	40.0%
!	CTTGTCAGCTGATTCTTGTT+TGG	-	Chr5:10641935-10641954	None:intergenic	40.0%
!	TCAGCTGATTCTTGTTTGGT+TGG	-	Chr5:10641931-10641950	None:intergenic	40.0%
!	TTATGCTGCTTGAAGTTTGC+AGG	+	Chr5:10641251-10641270	MsG0580024843.01.T01:intron	40.0%
!	TTTGGAATGGTCTGAACCTT+TGG	+	Chr5:10643245-10643264	MsG0580024843.01.T01:CDS	40.0%
!!	AAATTTGTTATCGGGCCACT+CGG	+	Chr5:10643643-10643662	MsG0580024843.01.T01:intron	40.0%
!!	GAGGCATATTTGGTTTGGAA+TGG	+	Chr5:10643232-10643251	MsG0580024843.01.T01:CDS	40.0%
!!	TTGGTTGGTGATTTCTTCAC+TGG	-	Chr5:10641916-10641935	None:intergenic	40.0%
!!!	CCGGTTTTGTTTGGTATCAT+AGG	+	Chr5:10643709-10643728	MsG0580024843.01.T01:intron	40.0%
	AAAAGGAGGGAGCAGGAAAA+TGG	+	Chr5:10641101-10641120	MsG0580024843.01.T01:CDS	45.0%
	AAGCGCTAAGCATAACTGTG+TGG	+	Chr5:10643506-10643525	MsG0580024843.01.T01:intron	45.0%
	AATGCCTTGAAAGAAGGGGA+AGG	+	Chr5:10644128-10644147	MsG0580024843.01.T01:CDS	45.0%
	ACAACCATTTCAGGACTCTC+AGG	-	Chr5:10642740-10642759	None:intergenic	45.0%
	ACATTAACAGCACCTCTCAC+TGG	-	Chr5:10644253-10644272	None:intergenic	45.0%
	ACTCGACTTTGCAAAGGACT+TGG	+	Chr5:10644045-10644064	MsG0580024843.01.T01:CDS	45.0%
	ACTTGCCTCTGTGAACTTGA+AGG	+	Chr5:10642472-10642491	MsG0580024843.01.T01:CDS	45.0%
	ATACCATATGGATCACGTGG+AGG	-	Chr5:10642238-10642257	None:intergenic	45.0%
	ATGCTTACTGCTAAGTGCGA+AGG	-	Chr5:10644433-10644452	None:intergenic	45.0%
	CAGATACCATATGGATCACG+TGG	-	Chr5:10642241-10642260	None:intergenic	45.0%
	CATACCTGAGAGTCCTGAAA+TGG	+	Chr5:10642733-10642752	MsG0580024843.01.T01:intron	45.0%
	CATTCGGTTGAAATCAACGG+TGG	-	Chr5:10642323-10642342	None:intergenic	45.0%
	CTGTCTACATTTCCTCCAAG+AGG	+	Chr5:10641647-10641666	MsG0580024843.01.T01:CDS	45.0%
	CTTACATGGGAAGGGAGATA+AGG	-	Chr5:10641375-10641394	None:intergenic	45.0%
	CTTCTGGCAAGAGAAATCTG+AGG	-	Chr5:10641781-10641800	None:intergenic	45.0%
	CTTGCCTCTGTGAACTTGAA+GGG	+	Chr5:10642473-10642492	MsG0580024843.01.T01:CDS	45.0%
	GCTACCTGTTGAACTGAGAA+AGG	+	Chr5:10644096-10644115	MsG0580024843.01.T01:CDS	45.0%
	GCTTCCTTTCTCAGTTCAAC+AGG	-	Chr5:10644103-10644122	None:intergenic	45.0%
	TCAGTTCAACAGGTAGCTGA+AGG	-	Chr5:10644093-10644112	None:intergenic	45.0%
	TGACAGGCCACAATAGTAGT+GGG	+	Chr5:10642980-10642999	MsG0580024843.01.T01:intron	45.0%
	TGATGGAATTCTGGTCCATC+GGG	-	Chr5:10641693-10641712	None:intergenic	45.0%
	TGGTGATTTCTTCACTGGAG+TGG	-	Chr5:10641911-10641930	None:intergenic	45.0%
	TTGCACTATCTTGTGGAAGC+TGG	+	Chr5:10643274-10643293	MsG0580024843.01.T01:CDS	45.0%
	TTGCTTGTACACCAGTAACC+TGG	+	Chr5:10644202-10644221	MsG0580024843.01.T01:exon	45.0%
!	AAGACCCTTCAAGTTCACAG+AGG	-	Chr5:10642480-10642499	None:intergenic	45.0%
!	AAGAGAAATCTGAGGCCTAG+TGG	-	Chr5:10641773-10641792	None:intergenic	45.0%
!	AGAGCACTCACTAGACCTTT+AGG	+	Chr5:10641330-10641349	MsG0580024843.01.T01:CDS	45.0%
!	CTTTTAGACAGGTCTGTTGC+AGG	-	Chr5:10643185-10643204	None:intergenic	45.0%
!!	ACCAAACAAAACCGGCTTGT+AGG	-	Chr5:10643704-10643723	None:intergenic	45.0%
!!	TGAAAAAAGGGCGTCGAGAA+AGG	+	Chr5:10641139-10641158	MsG0580024843.01.T01:CDS	45.0%
!!!	TCCTACAAGCCGGTTTTGTT+TGG	+	Chr5:10643700-10643719	MsG0580024843.01.T01:intron	45.0%
!!!	ATATATATTTTATTTTATGA+AGG	+	Chr5:10643895-10643914	MsG0580024843.01.T01:intron	5.0%
	ACACCTCCACGTGATCCATA+TGG	+	Chr5:10642232-10642251	MsG0580024843.01.T01:CDS	50.0%
	ACAGGCCACAATAGTAGTGG+GGG	+	Chr5:10642982-10643001	MsG0580024843.01.T01:intron	50.0%
	ACATGGGAAGGGAGATAAGG+AGG	-	Chr5:10641372-10641391	None:intergenic	50.0%
	ACCTTGCTGGATAATCCGAG+TGG	-	Chr5:10643661-10643680	None:intergenic	50.0%
	AGATGGAAAAAGGAGGGAGC+AGG	+	Chr5:10641094-10641113	MsG0580024843.01.T01:exon	50.0%
	CTTGAAGGGTCTTACTCACC+AGG	+	Chr5:10642487-10642506	MsG0580024843.01.T01:CDS	50.0%
	GACAGGCCACAATAGTAGTG+GGG	+	Chr5:10642981-10643000	MsG0580024843.01.T01:intron	50.0%
	GTGACAGGCCACAATAGTAG+TGG	+	Chr5:10642979-10642998	MsG0580024843.01.T01:intron	50.0%
	TAAGCCCTACTGTACCTTGC+TGG	-	Chr5:10643674-10643693	None:intergenic	50.0%
	TCGGTTGAAATCAACGGTGG+AGG	-	Chr5:10642320-10642339	None:intergenic	50.0%
	TGCAGATCACGTGAAGTTGC+AGG	+	Chr5:10641957-10641976	MsG0580024843.01.T01:CDS	50.0%
!	AGTTGCGGTATTGGAGGAAG+AGG	+	Chr5:10641570-10641589	MsG0580024843.01.T01:CDS	50.0%
!!	AGACGAGGAAGTGCTCCTAA+AGG	-	Chr5:10641348-10641367	None:intergenic	50.0%
!!	AGGGCGTCGAGAAAGGAAAA+TGG	+	Chr5:10641146-10641165	MsG0580024843.01.T01:CDS	50.0%
!!	GGTTGTAGCACTGATGCAGA+AGG	+	Chr5:10642754-10642773	MsG0580024843.01.T01:intron	50.0%
	AAGGCAGCCTCTTTCACACG+TGG	+	Chr5:10644147-10644166	MsG0580024843.01.T01:CDS	55.0%
	AGAGCTCGTGGCTGAAGTTG+CGG	+	Chr5:10641555-10641574	MsG0580024843.01.T01:CDS	55.0%
	CGTGGCTGAAGTTGCGGTAT+TGG	+	Chr5:10641561-10641580	MsG0580024843.01.T01:CDS	55.0%
	GCCACTCGGATTATCCAGCA+AGG	+	Chr5:10643657-10643676	MsG0580024843.01.T01:intron	55.0%
	GCTGCCTTCCCCTTCTTTCA+AGG	-	Chr5:10644135-10644154	None:intergenic	55.0%
	GGGAGATAAGGAGGAAGACG+AGG	-	Chr5:10641363-10641382	None:intergenic	55.0%
	GTGTCCCCCCACTACTATTG+TGG	-	Chr5:10642990-10643009	None:intergenic	55.0%
!	CAGGCCACAATAGTAGTGGG+GGG	+	Chr5:10642983-10643002	MsG0580024843.01.T01:intron	55.0%
!	GGCTGAAGTTGCGGTATTGG+AGG	+	Chr5:10641564-10641583	MsG0580024843.01.T01:CDS	55.0%
	AGTGGGGGGACACTTGCTCA+AGG	+	Chr5:10642997-10643016	MsG0580024843.01.T01:CDS	60.0%
	TGGAAGCTGGTCATCACCCG+TGG	+	Chr5:10643287-10643306	MsG0580024843.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	10640918	10644552	10640918	ID=MsG0580024843.01;Name=MsG0580024843.01
Chr5	mRNA	10640918	10644552	10640918	ID=MsG0580024843.01.T01;Parent=MsG0580024843.01;Name=MsG0580024843.01.T01;_AED=0.26;_eAED=0.27;_QI=178\|0.71\|0.75\|1\|0.85\|0.87\|8\|333\|525
Chr5	exon	10640918	10641182	10640918	ID=MsG0580024843.01.T01:exon:393;Parent=MsG0580024843.01.T01
Chr5	exon	10641273	10641389	10641273	ID=MsG0580024843.01.T01:exon:394;Parent=MsG0580024843.01.T01
Chr5	exon	10641550	10641978	10641550	ID=MsG0580024843.01.T01:exon:395;Parent=MsG0580024843.01.T01
Chr5	exon	10642067	10642374	10642067	ID=MsG0580024843.01.T01:exon:396;Parent=MsG0580024843.01.T01
Chr5	exon	10642457	10642545	10642457	ID=MsG0580024843.01.T01:exon:397;Parent=MsG0580024843.01.T01
Chr5	exon	10642986	10643069	10642986	ID=MsG0580024843.01.T01:exon:398;Parent=MsG0580024843.01.T01
Chr5	exon	10643148	10643308	10643148	ID=MsG0580024843.01.T01:exon:399;Parent=MsG0580024843.01.T01
Chr5	exon	10643917	10644552	10643917	ID=MsG0580024843.01.T01:exon:400;Parent=MsG0580024843.01.T01
Chr5	five_prime_UTR	10640918	10641095	10640918	ID=MsG0580024843.01.T01:five_prime_utr;Parent=MsG0580024843.01.T01
Chr5	CDS	10641096	10641182	10641096	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	CDS	10641273	10641389	10641273	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	CDS	10641550	10641978	10641550	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	CDS	10642067	10642374	10642067	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	CDS	10642457	10642545	10642457	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	CDS	10642986	10643069	10642986	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	CDS	10643148	10643308	10643148	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	CDS	10643917	10644219	10643917	ID=MsG0580024843.01.T01:cds;Parent=MsG0580024843.01.T01
Chr5	three_prime_UTR	10644220	10644552	10644220	ID=MsG0580024843.01.T01:three_prime_utr;Parent=MsG0580024843.01.T01

Gene Sequence

>MsG0580024843.01.T01

ATGGAAAAAGGAGGGAGCAGGAAAATGGCTGCACATAATACTTTGAAAAAAGGGCGTCGAGAAAGGAAAATGGCTTTGATACAAGATGTTGATAAGTTAAAGAGGAAGCTAAGACATGAAGAGAATGTTCATAGAGTGTTAGAAAGAGCACTCACTAGACCTTTAGGAGCACTTCCTCGTCTTCCTCCTTATCTCCCTTCCCATATGTTAGAGCTCGTGGCTGAAGTTGCGGTATTGGAGGAAGAGGTAGTTCGATTAGAAGAGAAGGTTGTGAATTTCCGACAAGGTTTATATCAGGAAGCTGTCTACATTTCCTCCAAGAGGAATGCAGAAAATTCGATTGACCCGATGGACCAGAATTCCATCAAAGGATCCAAACATCAAAGGTCAAAATCACTGTCTCAAAGTGAGCTTAATTCTACTACCACTAGGCCTCAGATTTCTCTTGCCAGAAGTGTTTCTAGTAGAAAGTTAGTAAATGGGAAACAATCGCATTCAAAACAAGATTCCTTATCATCAATACCAGAAGAGAAATCTATTGAAAAGAAAATGGCTAAAGTCACCACTCCAGTGAAGAAATCACCAACCAAACAAGAATCAGCTGACAAGTGTGCAGATCACGTGAAGTTGCAGCTGGAGAAGAGATTAGTAGATCAAGAAAGAGCACAAAATTCATCAGACGATAAGGTGTTAGAAGTTGATAGGACACCAAATAGAGTTTCAGAGGATATAGTCAAGTGTTTGTGTAGCATATTTATGAGAATTTCCATATTCAAGGACAATCTAGGAGAATCGAAAACACCTCCACGTGATCCATATGGTATCTGTTCAATATCGAAAACTAGAGATATTGGTGCCTACAATAGTTTTTGTGAGATTGAAGCCTCCACCGTTGATTTCAACCGAATGAAAAATTCAGTGTTTCTCATCAATCGATTAAAGTTTTTATTTGGGAAACTTGCCTCTGTGAACTTGAAGGGTCTTACTCACCAGGAGAAACTTGCATTTTGGATAAACACTTATAATTCCTGCCACAATAGTAGTGGGGGGACACTTGCTCAAGGCAATGACTATAGAGCACTTCATCTTGAGACTTCCTTATCACTTGAATTATATTACAATTCATCTCTTGATTTTCATCTTTGCTTCCTGCAACAGACCTGTCTAAAAGCTGCAAAAAATGATGAGATGAAAGCAAGAGGCATATTTGGTTTGGAATGGTCTGAACCTTTGGTTACATTTGCACTATCTTGTGGAAGCTGGTCATCACCCGTGGTAAGGGTGTACACAGCATCACAAGTTGATAATGAGTTAGAAGCAGCAAAGAGAGATTATTTACAGGCAGCAGTTGGCATTACAAAAACAAATAAGTTAATAATTCCAAAGATACTTGACTGGTTTTTACTCGACTTTGCAAAGGACTTGGAATCTTTGTTGGATTGGGTCTGCCTTCAGCTACCTGTTGAACTGAGAAAGGAAGCAGTTGAATGCCTTGAAAGAAGGGGAAGGCAGCCTCTTTCACACGTGGTACATATGATGCCCTATGATTTCAGTTTCAGGTTGCTTGTACACCAGTAA

Protein sequence

>MsG0580024843.01.T01

MEKGGSRKMAAHNTLKKGRRERKMALIQDVDKLKRKLRHEENVHRVLERALTRPLGALPRLPPYLPSHMLELVAEVAVLEEEVVRLEEKVVNFRQGLYQEAVYISSKRNAENSIDPMDQNSIKGSKHQRSKSLSQSELNSTTTRPQISLARSVSSRKLVNGKQSHSKQDSLSSIPEEKSIEKKMAKVTTPVKKSPTKQESADKCADHVKLQLEKRLVDQERAQNSSDDKVLEVDRTPNRVSEDIVKCLCSIFMRISIFKDNLGESKTPPRDPYGICSISKTRDIGAYNSFCEIEASTVDFNRMKNSVFLINRLKFLFGKLASVNLKGLTHQEKLAFWINTYNSCHNSSGGTLAQGNDYRALHLETSLSLELYYNSSLDFHLCFLQQTCLKAAKNDEMKARGIFGLEWSEPLVTFALSCGSWSSPVVRVYTASQVDNELEAAKRDYLQAAVGITKTNKLIIPKILDWFLLDFAKDLESLLDWVCLQLPVELRKEAVECLERRGRQPLSHVVHMMPYDFSFRLLVHQ*