AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005248.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005248.01.T01 MTR_1g095600 97.727 176 4 0 1 176 180 355 2.17e-122 349
MsG0180005248.01.T01 MTR_5g026910 58.523 176 65 2 1 176 190 357 1.13e-51 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005248.01.T01 AT4G25030 64.740 173 51 4 1 172 174 337 4.14e-69 213
MsG0180005248.01.T01 AT4G25030 64.740 173 51 4 1 172 174 337 4.14e-69 213
MsG0180005248.01.T01 AT5G45410 79.130 115 24 0 1 115 177 291 6.05e-60 190
MsG0180005248.01.T01 AT5G45410 79.130 115 24 0 1 115 177 291 6.05e-60 190
MsG0180005248.01.T01 AT5G45410 79.130 115 24 0 1 115 177 291 6.05e-60 190
MsG0180005248.01.T01 AT5G45410 79.130 115 24 0 1 115 177 291 6.05e-60 190
MsG0180005248.01.T01 AT5G45410 79.130 115 24 0 1 115 177 291 6.05e-60 190

Find 52 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTATTGCAGCATCAATTT+TGG 0.095240 1:-89158178 MsG0180005248.01.T01:CDS
CCGACAAAAGTTCTTGTGAT+TGG 0.288665 1:-89158354 MsG0180005248.01.T01:CDS
TGTTGGTTCGGTTCAGTTAC+CGG 0.302078 1:-89158037 MsG0180005248.01.T01:CDS
TGTTGGTTCGGTTCAGTTAC+CGG 0.302078 1:-89158073 MsG0180005248.01.T01:CDS
AGAAGCTGGATTCCACTTAA+TGG 0.381452 1:+89157978 None:intergenic
GTAAATGCCATCGCTGATTT+AGG 0.392350 1:+89158198 None:intergenic
GATGGCAGTTAATGTTCCCC+TGG 0.396744 1:-89158322 MsG0180005248.01.T01:CDS
CAGCTCTGGAGCCGATTACT+TGG 0.406356 1:+89158154 None:intergenic
TGAAACTTCTGATGTTGGTT+CGG 0.422639 1:-89158085 MsG0180005248.01.T01:CDS
TTAATGTTCCCCTGGGTGTA+TGG 0.431929 1:-89158314 MsG0180005248.01.T01:CDS
CGGTGCGTGCTATAGCCGCC+CGG 0.447654 1:+89158409 None:intergenic
AGTTCTTGTGATTGGAATGA+TGG 0.455110 1:-89158346 MsG0180005248.01.T01:CDS
GGTTAAACCTGATGTTGGTT+CGG 0.456336 1:-89158049 MsG0180005248.01.T01:CDS
GCAATTGAAACTTCTGATGT+TGG 0.457076 1:-89158090 MsG0180005248.01.T01:CDS
TTCCTAAGCATTGCTATGAA+TGG 0.459333 1:+89158234 None:intergenic
CACAGGTAATAGTTGAGGCC+CGG 0.459547 1:-89158428 MsG0180005248.01.T01:CDS
TTGCAGCAACCGCCTTCAAA+CGG 0.498713 1:+89158124 None:intergenic
AGCTGAGAGGTACCGTTTGA+AGG 0.501698 1:-89158136 MsG0180005248.01.T01:CDS
GCTGTCAGCTGAAGAGAAGC+TGG 0.505469 1:+89157964 None:intergenic
TTCCATTCATAGCAATGCTT+AGG 0.510376 1:-89158236 MsG0180005248.01.T01:CDS
ACAGGTAATAGTTGAGGCCC+GGG 0.513811 1:-89158427 MsG0180005248.01.T01:CDS
GTTCTTGTGATTGGAATGAT+GGG 0.518850 1:-89158345 MsG0180005248.01.T01:CDS
AACTGAACCGAACCAACATC+AGG 0.528631 1:+89158042 None:intergenic
CTGTCTTTAGATTTAACCAC+CGG 0.531229 1:+89158018 None:intergenic
CAAACGGTACCTCTCAGCTC+TGG 0.535101 1:+89158140 None:intergenic
CCGGTGGTTAAACCTGATGT+TGG 0.535233 1:-89158054 MsG0180005248.01.T01:CDS
ATGGCAGTTAATGTTCCCCT+GGG 0.538720 1:-89158321 MsG0180005248.01.T01:CDS
CGGGCCTCAACTATTACCTG+TGG 0.551578 1:+89158429 None:intergenic
GGGTGTATGGAGAGAACATA+CGG 0.582703 1:-89158301 MsG0180005248.01.T01:CDS
CGGTGGTTAAATCTAAAGAC+AGG 0.591640 1:-89158017 MsG0180005248.01.T01:CDS
AGACAGGCACTGTGGTGATG+AGG 0.596413 1:-89158001 MsG0180005248.01.T01:CDS
GGTGCGTGCTATAGCCGCCC+GGG 0.604464 1:+89158410 None:intergenic
ATGTTCTCTCCATACACCCA+GGG 0.609222 1:+89158305 None:intergenic
GGGCCTCAACTATTACCTGT+GGG 0.612298 1:+89158430 None:intergenic
GCGGAGATTCTTTGCAAATG+CGG 0.615724 1:+89158389 None:intergenic
TATGTTCTCTCCATACACCC+AGG 0.620444 1:+89158304 None:intergenic
CTTGATGCCTAAATCAGCGA+TGG 0.629945 1:-89158205 MsG0180005248.01.T01:CDS
TGTGATTGGAATGATGGGGA+TGG 0.631056 1:-89158340 MsG0180005248.01.T01:CDS
CTATGAATGGAACAGCTGCA+TGG 0.632725 1:+89158247 None:intergenic
GTGGTGATGAGGCCATTAAG+TGG 0.636840 1:-89157990 MsG0180005248.01.T01:CDS
TATGAAATACAAGTGCCCAC+AGG 0.637852 1:-89158445 MsG0180005248.01.T01:CDS
CCAACATCAGGTTTAACCAC+CGG 0.638938 1:+89158054 None:intergenic
GTGCCCACAGGTAATAGTTG+AGG 0.641293 1:-89158433 MsG0180005248.01.T01:CDS
TGAGAGGTACCGTTTGAAGG+CGG 0.674519 1:-89158133 MsG0180005248.01.T01:CDS
TGGACAGCGACAAACCATGA+CGG 0.683212 1:+89158267 None:intergenic
TGGTTCGGTTCAGTTACCGG+TGG 0.691322 1:-89158034 MsG0180005248.01.T01:CDS
TGGTTCGGTTCAGTTACCGG+TGG 0.691322 1:-89158070 MsG0180005248.01.T01:CDS
TAATCGGCTCCAGAGCTGAG+AGG 0.712400 1:-89158149 MsG0180005248.01.T01:CDS
TTCTTGTGATTGGAATGATG+GGG 0.717293 1:-89158344 MsG0180005248.01.T01:CDS
AAATCTAAAGACAGGCACTG+TGG 0.728626 1:-89158009 MsG0180005248.01.T01:CDS
GGTAATAGTTGAGGCCCGGG+CGG 0.745364 1:-89158424 MsG0180005248.01.T01:CDS
TGTTCTCTCCATACACCCAG+GGG 0.753131 1:+89158306 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACTATTGCAGCATCAATTTT+GGG - Chr1:89158203-89158222 MsG0180005248.01.T01:CDS 30.0%
! TACTATTGCAGCATCAATTT+TGG - Chr1:89158202-89158221 MsG0180005248.01.T01:CDS 30.0%
AGTTCTTGTGATTGGAATGA+TGG - Chr1:89158034-89158053 MsG0180005248.01.T01:CDS 35.0%
CTGTCTTTAGATTTAACCAC+CGG + Chr1:89158365-89158384 None:intergenic 35.0%
GCAATTGAAACTTCTGATGT+TGG - Chr1:89158290-89158309 MsG0180005248.01.T01:CDS 35.0%
GTTCTTGTGATTGGAATGAT+GGG - Chr1:89158035-89158054 MsG0180005248.01.T01:CDS 35.0%
TTCCATTCATAGCAATGCTT+AGG - Chr1:89158144-89158163 MsG0180005248.01.T01:CDS 35.0%
TTCTTGTGATTGGAATGATG+GGG - Chr1:89158036-89158055 MsG0180005248.01.T01:CDS 35.0%
! CCAATCACAAGAACTTTTGT+CGG + Chr1:89158029-89158048 None:intergenic 35.0%
! TCAATTTTGGGCCAAGTAAT+CGG - Chr1:89158215-89158234 MsG0180005248.01.T01:CDS 35.0%
! TGAAACTTCTGATGTTGGTT+CGG - Chr1:89158295-89158314 MsG0180005248.01.T01:CDS 35.0%
! TTCCTAAGCATTGCTATGAA+TGG + Chr1:89158149-89158168 None:intergenic 35.0%
AGAAGCTGGATTCCACTTAA+TGG + Chr1:89158405-89158424 None:intergenic 40.0%
CCGACAAAAGTTCTTGTGAT+TGG - Chr1:89158026-89158045 MsG0180005248.01.T01:CDS 40.0%
CGGTGGTTAAATCTAAAGAC+AGG - Chr1:89158363-89158382 MsG0180005248.01.T01:CDS 40.0%
! AAATCTAAAGACAGGCACTG+TGG - Chr1:89158371-89158390 MsG0180005248.01.T01:CDS 40.0%
! GGTTAAACCTGATGTTGGTT+CGG - Chr1:89158331-89158350 MsG0180005248.01.T01:CDS 40.0%
!! GTAAATGCCATCGCTGATTT+AGG + Chr1:89158185-89158204 None:intergenic 40.0%
AACTGAACCGAACCAACATC+AGG + Chr1:89158305-89158324 None:intergenic 45.0%
ATGGCAGTTAATGTTCCCCT+GGG - Chr1:89158059-89158078 MsG0180005248.01.T01:CDS 45.0%
ATGTTCTCTCCATACACCCA+GGG + Chr1:89158078-89158097 None:intergenic 45.0%
CCAACATCAGGTTTAACCAC+CGG + Chr1:89158329-89158348 None:intergenic 45.0%
CTATGAATGGAACAGCTGCA+TGG + Chr1:89158136-89158155 None:intergenic 45.0%
CTTGATGCCTAAATCAGCGA+TGG - Chr1:89158175-89158194 MsG0180005248.01.T01:CDS 45.0%
GCGGAGATTCTTTGCAAATG+CGG + Chr1:89157994-89158013 None:intergenic 45.0%
GGGTGTATGGAGAGAACATA+CGG - Chr1:89158079-89158098 MsG0180005248.01.T01:CDS 45.0%
TATGTTCTCTCCATACACCC+AGG + Chr1:89158079-89158098 None:intergenic 45.0%
TTAATGTTCCCCTGGGTGTA+TGG - Chr1:89158066-89158085 MsG0180005248.01.T01:CDS 45.0%
! CGGAGAAATTTTCACCGTCA+TGG - Chr1:89158099-89158118 MsG0180005248.01.T01:CDS 45.0%
! TGTGATTGGAATGATGGGGA+TGG - Chr1:89158040-89158059 MsG0180005248.01.T01:CDS 45.0%
!! TGTTGGTTCGGTTCAGTTAC+CGG - Chr1:89158307-89158326 MsG0180005248.01.T01:CDS 45.0%
ACAGGTAATAGTTGAGGCCC+GGG - Chr1:89157953-89157972 MsG0180005248.01.T01:CDS 50.0%
CACAGGTAATAGTTGAGGCC+CGG - Chr1:89157952-89157971 MsG0180005248.01.T01:CDS 50.0%
CCGGTGGTTAAACCTGATGT+TGG - Chr1:89158326-89158345 MsG0180005248.01.T01:CDS 50.0%
GATGGCAGTTAATGTTCCCC+TGG - Chr1:89158058-89158077 MsG0180005248.01.T01:CDS 50.0%
GGGCCTCAACTATTACCTGT+GGG + Chr1:89157953-89157972 None:intergenic 50.0%
GTGCCCACAGGTAATAGTTG+AGG - Chr1:89157947-89157966 MsG0180005248.01.T01:CDS 50.0%
TGGACAGCGACAAACCATGA+CGG + Chr1:89158116-89158135 None:intergenic 50.0%
TGTTCTCTCCATACACCCAG+GGG + Chr1:89158077-89158096 None:intergenic 50.0%
TTGCAGCAACCGCCTTCAAA+CGG + Chr1:89158259-89158278 None:intergenic 50.0%
! AACTTTTGTCGGCAAGCCCT+GGG + Chr1:89158018-89158037 None:intergenic 50.0%
! GTGGTGATGAGGCCATTAAG+TGG - Chr1:89158390-89158409 MsG0180005248.01.T01:CDS 50.0%
!! AGCTGAGAGGTACCGTTTGA+AGG - Chr1:89158244-89158263 MsG0180005248.01.T01:CDS 50.0%
!! TGAGAGGTACCGTTTGAAGG+CGG - Chr1:89158247-89158266 MsG0180005248.01.T01:CDS 50.0%
CAAACGGTACCTCTCAGCTC+TGG + Chr1:89158243-89158262 None:intergenic 55.0%
CGGGCCTCAACTATTACCTG+TGG + Chr1:89157954-89157973 None:intergenic 55.0%
GCTGTCAGCTGAAGAGAAGC+TGG + Chr1:89158419-89158438 None:intergenic 55.0%
! ACTTTTGTCGGCAAGCCCTG+GGG + Chr1:89158017-89158036 None:intergenic 55.0%
! AGACAGGCACTGTGGTGATG+AGG - Chr1:89158379-89158398 MsG0180005248.01.T01:CDS 55.0%
! GAACTTTTGTCGGCAAGCCC+TGG + Chr1:89158019-89158038 None:intergenic 55.0%
! TAATCGGCTCCAGAGCTGAG+AGG - Chr1:89158231-89158250 MsG0180005248.01.T01:CDS 55.0%
!! CAGCTCTGGAGCCGATTACT+TGG + Chr1:89158229-89158248 None:intergenic 55.0%
!! TGGTTCGGTTCAGTTACCGG+TGG - Chr1:89158310-89158329 MsG0180005248.01.T01:CDS 55.0%
GCAAAGAATCTCCGCCCCCA+GGG - Chr1:89157999-89158018 MsG0180005248.01.T01:CDS 60.0%
GGTAATAGTTGAGGCCCGGG+CGG - Chr1:89157956-89157975 MsG0180005248.01.T01:CDS 60.0%
TGCAAAGAATCTCCGCCCCC+AGG - Chr1:89157998-89158017 MsG0180005248.01.T01:CDS 60.0%
! CTTTTGTCGGCAAGCCCTGG+GGG + Chr1:89158016-89158035 None:intergenic 60.0%
CGGTGCGTGCTATAGCCGCC+CGG + Chr1:89157974-89157993 None:intergenic 70.0%
GGTGCGTGCTATAGCCGCCC+GGG + Chr1:89157973-89157992 None:intergenic 70.0%
TTGTCGGCAAGCCCTGGGGG+CGG + Chr1:89158013-89158032 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 89157936 89158466 89157936 ID=MsG0180005248.01;Name=MsG0180005248.01
Chr1 mRNA 89157936 89158466 89157936 ID=MsG0180005248.01.T01;Parent=MsG0180005248.01;Name=MsG0180005248.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|176
Chr1 exon 89157936 89158466 89157936 ID=MsG0180005248.01.T01:exon:14566;Parent=MsG0180005248.01.T01
Chr1 CDS 89157936 89158466 89157936 ID=MsG0180005248.01.T01:cds;Parent=MsG0180005248.01.T01
Gene Sequence

>MsG0180005248.01.T01

ATGAAATACAAGTGCCCACAGGTAATAGTTGAGGCCCGGGCGGCTATAGCACGCACCGCATTTGCAAAGAATCTCCGCCCCCAGGGCTTGCCGACAAAAGTTCTTGTGATTGGAATGATGGGGATGGCAGTTAATGTTCCCCTGGGTGTATGGAGAGAACATACGGAGAAATTTTCACCGTCATGGTTTGTCGCTGTCCATGCAGCTGTTCCATTCATAGCAATGCTTAGGAAGTCAGTCTTGATGCCTAAATCAGCGATGGCATTTACTATTGCAGCATCAATTTTGGGCCAAGTAATCGGCTCCAGAGCTGAGAGGTACCGTTTGAAGGCGGTTGCTGCAAAAAAGTTGTCTGCAATTGAAACTTCTGATGTTGGTTCGGTTCAGTTACCGGTGGTTAAACCTGATGTTGGTTCGGTTCAGTTACCGGTGGTTAAATCTAAAGACAGGCACTGTGGTGATGAGGCCATTAAGTGGAATCCAGCTTCTCTTCAGCTGACAGCAACTTCATCAACTGATGTATTTTGTTGA

Protein sequence

>MsG0180005248.01.T01

MKYKCPQVIVEARAAIARTAFAKNLRPQGLPTKVLVIGMMGMAVNVPLGVWREHTEKFSPSWFVAVHAAVPFIAMLRKSVLMPKSAMAFTIAASILGQVIGSRAERYRLKAVAAKKLSAIETSDVGSVQLPVVKPDVGSVQLPVVKSKDRHCGDEAIKWNPASLQLTATSSTDVFC*