Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027136.01.T01 | XP_003619775.1 | 98.182 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 8.23E-74 | 224 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027136.01.T01 | G7KPB8 | 98.182 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 3.93e-74 | 224 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048152.01 | MsG0580027136.01 | 0.802110 | 6.553154e-49 | 3.183292e-46 |
| MsG0180000836.01 | MsG0580027136.01 | 0.812647 | 3.816599e-51 | 2.440038e-48 |
| MsG0180002547.01 | MsG0580027136.01 | 0.815448 | 9.198565e-52 | 6.343740e-49 |
| MsG0180002664.01 | MsG0580027136.01 | 0.809952 | 1.467398e-50 | 8.733403e-48 |
| MsG0180002794.01 | MsG0580027136.01 | 0.834299 | 3.248471e-56 | 3.839127e-53 |
| MsG0180003922.01 | MsG0580027136.01 | 0.808829 | 2.554882e-50 | 1.475813e-47 |
| MsG0180005153.01 | MsG0580027136.01 | 0.803270 | 3.775946e-49 | 1.889329e-46 |
| MsG0180005169.01 | MsG0580027136.01 | 0.823953 | 1.049540e-53 | 9.169644e-51 |
| MsG0180006096.01 | MsG0580027136.01 | 0.818785 | 1.635463e-52 | 1.235974e-49 |
| MsG0280010778.01 | MsG0580027136.01 | 0.803947 | 2.733929e-49 | 1.392023e-46 |
| MsG0380011581.01 | MsG0580027136.01 | 0.816517 | 5.308209e-52 | 3.769409e-49 |
| MsG0480020024.01 | MsG0580027136.01 | 0.801273 | 9.731105e-49 | 4.628014e-46 |
| MsG0480020675.01 | MsG0580027136.01 | 0.829848 | 4.092184e-55 | 4.239368e-52 |
| MsG0480020780.01 | MsG0580027136.01 | 0.802257 | 6.114162e-49 | 2.981231e-46 |
| MsG0480023221.01 | MsG0580027136.01 | 0.809162 | 2.168870e-50 | 1.264080e-47 |
| MsG0580025651.01 | MsG0580027136.01 | 0.812817 | 3.502940e-51 | 2.249364e-48 |
| MsG0380016133.01 | MsG0580027136.01 | 0.804340 | 2.264205e-49 | 1.164495e-46 |
| MsG0380018033.01 | MsG0580027136.01 | 0.807798 | 4.239105e-50 | 2.383335e-47 |
| MsG0580027136.01 | MsG0580028582.01 | 0.810719 | 1.002243e-50 | 6.087126e-48 |
| MsG0580027136.01 | MsG0580029736.01 | 0.802379 | 5.769474e-49 | 2.822089e-46 |
| MsG0580027136.01 | MsG0680035888.01 | 0.831063 | 2.063860e-55 | 2.214939e-52 |
| MsG0580027136.01 | MsG0780035937.01 | 0.803161 | 3.978432e-49 | 1.985103e-46 |
| MsG0580027136.01 | MsG0780036659.01 | 0.806383 | 8.447449e-50 | 4.578544e-47 |
| MsG0580027136.01 | MsG0780039170.01 | 0.814513 | 1.482547e-51 | 9.968543e-49 |
| MsG0580027136.01 | MsG0780039443.01 | 0.804574 | 2.023986e-49 | 1.047215e-46 |
| MsG0580027136.01 | MsG0780040430.01 | 0.808186 | 3.504468e-50 | 1.990630e-47 |
| MsG0580027136.01 | MsG0780041387.01 | 0.800880 | 1.171122e-48 | 5.514777e-46 |
| MsG0580027136.01 | MsG0780041435.01 | 0.823113 | 1.649517e-53 | 1.407409e-50 |
| MsG0580027136.01 | MsG0880042033.01 | 0.801942 | 7.096472e-49 | 3.432457e-46 |
| MsG0580027136.01 | MsG0880042760.01 | 0.818908 | 1.532921e-52 | 1.162559e-49 |
| MsG0580027136.01 | MsG0880044975.01 | 0.803237 | 3.836418e-49 | 1.917926e-46 |
| MsG0580027136.01 | MsG0880046901.01 | 0.801943 | 7.094250e-49 | 3.431419e-46 |
| MsG0280007527.01 | MsG0580027136.01 | 0.824866 | 6.397768e-54 | 5.737672e-51 |
| MsG0280007575.01 | MsG0580027136.01 | 0.804432 | 2.166495e-49 | 1.116854e-46 |
| MsG0280008113.01 | MsG0580027136.01 | 0.800660 | 1.298379e-48 | 6.079916e-46 |
| MsG0280008765.01 | MsG0580027136.01 | 0.809505 | 1.830689e-50 | 1.076757e-47 |
| MsG0280009575.01 | MsG0580027136.01 | 0.809818 | 1.567917e-50 | 9.299028e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027136.01.T01 | MTR_6g068850 | 98.182 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 9.96e-78 | 224 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027136.01.T01 | AT5G51960 | 59.091 | 110 | 38 | 1 | 1 | 110 | 1 | 103 | 9.89e-42 | 133 |
| MsG0580027136.01.T01 | AT5G51960 | 59.091 | 110 | 38 | 1 | 1 | 110 | 1 | 103 | 9.89e-42 | 133 |
Find 21 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATACTTACATGCTTAA+TGG | 0.209140 | 5:-54225676 | MsG0580027136.01.T01:CDS |
| AAGGCGGTCAAGAAACATAT+TGG | 0.364646 | 5:-54225808 | MsG0580027136.01.T01:CDS |
| GGCGATAGATGTTAACAGTT+TGG | 0.372669 | 5:+54225839 | None:intergenic |
| TCTTGACCGCCTTGAGAAGA+TGG | 0.432707 | 5:+54225818 | None:intergenic |
| CCTCAGAGTTCCACAAGAAT+CGG | 0.468221 | 5:-54225747 | MsG0580027136.01.T01:CDS |
| AGGCGGTCAAGAAACATATT+GGG | 0.477390 | 5:-54225807 | MsG0580027136.01.T01:CDS |
| ATAGATGTTAACAGTTTGGA+AGG | 0.532971 | 5:+54225843 | None:intergenic |
| CATTATGATTGATAAACCTC+GGG | 0.535278 | 5:+54225164 | None:intergenic |
| GGAAAATCTGCTGCAAGTGT+TGG | 0.560044 | 5:-54225197 | MsG0580027136.01.T01:CDS |
| CAGACAGATTCCGATTCTTG+TGG | 0.562091 | 5:+54225737 | None:intergenic |
| TCATTATGATTGATAAACCT+CGG | 0.563220 | 5:+54225163 | None:intergenic |
| CATCTATCGCCATCTTCTCA+AGG | 0.575149 | 5:-54225827 | MsG0580027136.01.T01:CDS |
| AATGAAGTGAAAAGAACACT+TGG | 0.586563 | 5:-54225218 | MsG0580027136.01.T01:CDS |
| TCGGAATCTGTCTGATGGTG+TGG | 0.587780 | 5:-54225728 | MsG0580027136.01.T01:CDS |
| CCGATTCTTGTGGAACTCTG+AGG | 0.596469 | 5:+54225747 | None:intergenic |
| AACAAAGCAATACCTTGTGA+TGG | 0.597418 | 5:+54225647 | None:intergenic |
| AAGAATCGGAATCTGTCTGA+TGG | 0.612539 | 5:-54225733 | MsG0580027136.01.T01:CDS |
| TAATGGTGTGCACCATCACA+AGG | 0.617495 | 5:-54225659 | MsG0580027136.01.T01:intron |
| GATAAACCTCGGGAAGCTGA+AGG | 0.641065 | 5:+54225174 | None:intergenic |
| TGTTGGCCTTCAGCTTCCCG+AGG | 0.654165 | 5:-54225180 | MsG0580027136.01.T01:CDS |
| CTATCGCCATCTTCTCAAGG+CGG | 0.739647 | 5:-54225824 | MsG0580027136.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTAGCAAAACAAAATG+TGG | + | Chr5:54225713-54225732 | None:intergenic | 20.0% |
| ! | AGATTAATGACAATGATTCA+TGG | + | Chr5:54225740-54225759 | None:intergenic | 25.0% |
| ! | AGTTTATCTCATTAATGTAC+AGG | + | Chr5:54225443-54225462 | None:intergenic | 25.0% |
| ! | GATTATACTTACATGCTTAA+TGG | - | Chr5:54225346-54225365 | MsG0580027136.01.T01:intron | 25.0% |
| AATACAAGTGACTGTTTGAA+GGG | + | Chr5:54225414-54225433 | None:intergenic | 30.0% | |
| AATGAAGTGAAAAGAACACT+TGG | - | Chr5:54225804-54225823 | MsG0580027136.01.T01:CDS | 30.0% | |
| ATAGATGTTAACAGTTTGGA+AGG | + | Chr5:54225182-54225201 | None:intergenic | 30.0% | |
| ATCATTGTCATTAATCTTGC+AGG | - | Chr5:54225743-54225762 | MsG0580027136.01.T01:CDS | 30.0% | |
| TAAGACAATCTTCAGAGTAT+TGG | + | Chr5:54225615-54225634 | None:intergenic | 30.0% | |
| TGTTGCGAAATAAAAGTAAG+AGG | - | Chr5:54225519-54225538 | MsG0580027136.01.T01:intron | 30.0% | |
| AACAAAGCAATACCTTGTGA+TGG | + | Chr5:54225378-54225397 | None:intergenic | 35.0% | |
| CAATACAAGTGACTGTTTGA+AGG | + | Chr5:54225415-54225434 | None:intergenic | 35.0% | |
| CTGTAGCGTAAATCATTACT+AGG | - | Chr5:54225680-54225699 | MsG0580027136.01.T01:CDS | 35.0% | |
| ! | AAAAGTAAGAGGTGTAGAAG+TGG | - | Chr5:54225530-54225549 | MsG0580027136.01.T01:intron | 35.0% |
| ! | CTCTGAAGATTGTCTTACAA+TGG | - | Chr5:54225618-54225637 | MsG0580027136.01.T01:intron | 35.0% |
| !!! | GAGCAAGCTTTATTTTCTGT+TGG | + | Chr5:54225324-54225343 | None:intergenic | 35.0% |
| AAGAATCGGAATCTGTCTGA+TGG | - | Chr5:54225289-54225308 | MsG0580027136.01.T01:intron | 40.0% | |
| AAGGCGGTCAAGAAACATAT+TGG | - | Chr5:54225214-54225233 | MsG0580027136.01.T01:CDS | 40.0% | |
| AGGCGGTCAAGAAACATATT+GGG | - | Chr5:54225215-54225234 | MsG0580027136.01.T01:CDS | 40.0% | |
| GGCGATAGATGTTAACAGTT+TGG | + | Chr5:54225186-54225205 | None:intergenic | 40.0% | |
| !! | CAATCTTCAGAGTATTGGCT+TGG | + | Chr5:54225610-54225629 | None:intergenic | 40.0% |
| CAGACAGATTCCGATTCTTG+TGG | + | Chr5:54225288-54225307 | None:intergenic | 45.0% | |
| CATCTATCGCCATCTTCTCA+AGG | - | Chr5:54225195-54225214 | MsG0580027136.01.T01:CDS | 45.0% | |
| CCTCAGAGTTCCACAAGAAT+CGG | - | Chr5:54225275-54225294 | MsG0580027136.01.T01:intron | 45.0% | |
| GCAATTCAGCATAGCCAAGT+TGG | - | Chr5:54225582-54225601 | MsG0580027136.01.T01:intron | 45.0% | |
| GGAAAATCTGCTGCAAGTGT+TGG | - | Chr5:54225825-54225844 | MsG0580027136.01.T01:CDS | 45.0% | |
| TAATGGTGTGCACCATCACA+AGG | - | Chr5:54225363-54225382 | MsG0580027136.01.T01:intron | 45.0% | |
| !! | GTATTGGCTTGGATCCAACT+TGG | + | Chr5:54225599-54225618 | None:intergenic | 45.0% |
| CCGATTCTTGTGGAACTCTG+AGG | + | Chr5:54225278-54225297 | None:intergenic | 50.0% | |
| CTATCGCCATCTTCTCAAGG+CGG | - | Chr5:54225198-54225217 | MsG0580027136.01.T01:CDS | 50.0% | |
| GATAAACCTCGGGAAGCTGA+AGG | + | Chr5:54225851-54225870 | None:intergenic | 50.0% | |
| TCTTGACCGCCTTGAGAAGA+TGG | + | Chr5:54225207-54225226 | None:intergenic | 50.0% | |
| ! | TCGGAATCTGTCTGATGGTG+TGG | - | Chr5:54225294-54225313 | MsG0580027136.01.T01:intron | 50.0% |
| !! | TGTTGGCCTTCAGCTTCCCG+AGG | - | Chr5:54225842-54225861 | MsG0580027136.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 54225166 | 54225878 | 54225166 | ID=MsG0580027136.01;Name=MsG0580027136.01 |
| Chr5 | mRNA | 54225166 | 54225878 | 54225166 | ID=MsG0580027136.01.T01;Parent=MsG0580027136.01;Name=MsG0580027136.01.T01;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|110 |
| Chr5 | exon | 54225660 | 54225878 | 54225660 | ID=MsG0580027136.01.T01:exon:40553;Parent=MsG0580027136.01.T01 |
| Chr5 | exon | 54225166 | 54225279 | 54225166 | ID=MsG0580027136.01.T01:exon:40554;Parent=MsG0580027136.01.T01 |
| Chr5 | CDS | 54225660 | 54225878 | 54225660 | ID=MsG0580027136.01.T01:cds;Parent=MsG0580027136.01.T01 |
| Chr5 | CDS | 54225166 | 54225279 | 54225166 | ID=MsG0580027136.01.T01:cds;Parent=MsG0580027136.01.T01 |
| Gene Sequence |
| Protein sequence |
