AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028582.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028582.01.T01 MTR_5g074880 94.074 135 8 0 1 135 1 135 4.34e-91 260
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028582.01.T01 AT2G30330 59.649 114 46 0 22 135 37 150 1.18e-46 148
MsG0580028582.01.T01 AT2G30330 48.039 102 52 1 22 123 37 137 1.06e-28 102

Find 23 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGCCTTGTATCTTTATTC+TGG 0.213211 5:-82785056 MsG0580028582.01.T01:three_prime_UTR
TTTGATGATGAATGTGAATT+AGG 0.250869 5:+82787279 None:intergenic
TGTAGCACTGGTTGGTGATT+TGG 0.356599 5:-82784986 MsG0580028582.01.T01:three_prime_UTR
TACGTATATTTGACAAATCA+TGG 0.369629 5:-82784961 MsG0580028582.01.T01:three_prime_UTR
AAATTGGAGACTTTGAGAAT+TGG 0.397575 5:-82785298 MsG0580028582.01.T01:CDS
TTCACCAAGAGTGATCTTGC+AGG 0.449401 5:-82785223 MsG0580028582.01.T01:exon
ACGCGTCTCGGATCTGTTAG+TGG 0.461253 5:-82787195 MsG0580028582.01.T01:CDS
TGAGAATTGGATGAAGATCA+TGG 0.464193 5:-82785285 MsG0580028582.01.T01:CDS
GGCTTCTAATGATGTAGCAC+TGG 0.472814 5:-82784998 MsG0580028582.01.T01:three_prime_UTR
TTCGAGATTTGTGAAACAAA+CGG 0.473259 5:-82787090 MsG0580028582.01.T01:CDS
TTGTTTCTCAACAAAATAAG+TGG 0.494592 5:-82785091 MsG0580028582.01.T01:three_prime_UTR
TTCTCTTAACACTGCTCTCA+AGG 0.509830 5:-82787045 MsG0580028582.01.T01:intron
ACGCGCAGAATCAAAGGGAT+TGG 0.518596 5:-82787318 MsG0580028582.01.T01:CDS
AAGAAAAGCAGAACGCGTCT+CGG 0.526602 5:-82787207 MsG0580028582.01.T01:CDS
TAACACACGCGCAGAATCAA+AGG 0.527349 5:-82787324 MsG0580028582.01.T01:CDS
AATACAAAGAAACAAGTGAA+TGG 0.531661 5:-82787360 None:intergenic
GCAACCTGCAAGATCACTCT+TGG 0.532927 5:+82785219 None:intergenic
CTGTTAGTGGAAGCAGTGAA+TGG 0.540308 5:-82787182 MsG0580028582.01.T01:CDS
TCTAATGATGTAGCACTGGT+TGG 0.573738 5:-82784994 MsG0580028582.01.T01:three_prime_UTR
AACACACGCGCAGAATCAAA+GGG 0.580106 5:-82787323 MsG0580028582.01.T01:CDS
TTGTGAAACAAACGGATCAG+TGG 0.597090 5:-82787082 MsG0580028582.01.T01:CDS
ACATCCAGAATAAAGATACA+AGG 0.617763 5:+82785052 None:intergenic
TTAGTGGAAGCAGTGAATGG+CGG 0.745571 5:-82787179 MsG0580028582.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAGTTAATAATGATA+AGG - Chr5:82785496-82785515 MsG0580028582.01.T01:intron 10.0%
!!! TGTAAAATTTTATTAGATTA+AGG - Chr5:82787194-82787213 MsG0580028582.01.T01:CDS 10.0%
!! AATAGAAAAATTAATGGAAA+GGG + Chr5:82787152-82787171 None:intergenic 15.0%
!!! AATAAAATTTTACATGATGT+TGG + Chr5:82787188-82787207 None:intergenic 15.0%
!!! ATAAAATTTTACATGATGTT+GGG + Chr5:82787187-82787206 None:intergenic 15.0%
!!! TTTTAAATGATGAATATGAT+TGG - Chr5:82787290-82787309 MsG0580028582.01.T01:CDS 15.0%
!! AATATGATTCCACAATATAA+AGG + Chr5:82786198-82786217 None:intergenic 20.0%
!! GAATAGAAAAATTAATGGAA+AGG + Chr5:82787153-82787172 None:intergenic 20.0%
!! TTTGTAAATGTTTATTCTTG+TGG - Chr5:82786420-82786439 MsG0580028582.01.T01:intron 20.0%
!!! AAACTTTTTTTTTAACTCGT+AGG - Chr5:82786988-82787007 MsG0580028582.01.T01:intron 20.0%
!!! AAATGTCACAAAAACAATTT+TGG - Chr5:82785974-82785993 MsG0580028582.01.T01:intron 20.0%
!!! CAAAATAATTTTGGTTATGA+TGG - Chr5:82786362-82786381 MsG0580028582.01.T01:intron 20.0%
!!! CATCATAACCAAAATTATTT+TGG + Chr5:82786364-82786383 None:intergenic 20.0%
!!! TCCATTAATTTTTCTATTCT+AGG - Chr5:82787154-82787173 MsG0580028582.01.T01:CDS 20.0%
! ATATGAGCAAGAATAAAGAT+GGG - Chr5:82786823-82786842 MsG0580028582.01.T01:intron 25.0%
! CTTCTCTTCAAATATTCATT+GGG - Chr5:82786527-82786546 MsG0580028582.01.T01:intron 25.0%
! GAAGTTAGATACTAAATTGA+CGG - Chr5:82785759-82785778 MsG0580028582.01.T01:intron 25.0%
! GCCTAGAATAGAAAAATTAA+TGG + Chr5:82787158-82787177 None:intergenic 25.0%
! TACGTATATTTGACAAATCA+TGG - Chr5:82787348-82787367 MsG0580028582.01.T01:exon 25.0%
! TCTTCTCTTCAAATATTCAT+TGG - Chr5:82786526-82786545 MsG0580028582.01.T01:intron 25.0%
! TTAAGGTTGTTAAAGATTCA+AGG - Chr5:82786649-82786668 MsG0580028582.01.T01:intron 25.0%
! TTTAATAATCCACTACCAAT+TGG - Chr5:82786049-82786068 MsG0580028582.01.T01:intron 25.0%
! TTTGAAAGAACACTGATAAA+TGG - Chr5:82786753-82786772 MsG0580028582.01.T01:intron 25.0%
!! ATTGATAAGTTAGCTTATAG+CGG - Chr5:82785379-82785398 MsG0580028582.01.T01:intron 25.0%
!! TAGTTTATGGACTAAATTGA+TGG - Chr5:82785821-82785840 MsG0580028582.01.T01:intron 25.0%
!! TTCCTTCTCCAAAATAATTT+TGG - Chr5:82786353-82786372 MsG0580028582.01.T01:intron 25.0%
!! TTGTTTCTCAACAAAATAAG+TGG - Chr5:82787218-82787237 MsG0580028582.01.T01:CDS 25.0%
!!! AACCAAAATTATTTTGGAGA+AGG + Chr5:82786358-82786377 None:intergenic 25.0%
!!! TGGTAGTGGATTATTAAATT+GGG + Chr5:82786047-82786066 None:intergenic 25.0%
!!! TTGGTAGTGGATTATTAAAT+TGG + Chr5:82786048-82786067 None:intergenic 25.0%
!!! TTTGATGATGAATGTGAATT+AGG + Chr5:82785033-82785052 None:intergenic 25.0%
!!! TTTTTTAACTCGTAGGAAAT+TGG - Chr5:82786995-82787014 MsG0580028582.01.T01:intron 25.0%
ACATAACATACTTCCATTCA+GGG + Chr5:82786283-82786302 None:intergenic 30.0%
ACATCCAGAATAAAGATACA+AGG + Chr5:82787260-82787279 None:intergenic 30.0%
ATTCACCAATAACCCAAAAA+TGG + Chr5:82786396-82786415 None:intergenic 30.0%
CATATGAGCAAGAATAAAGA+TGG - Chr5:82786822-82786841 MsG0580028582.01.T01:intron 30.0%
CATTAACATGAACATGTACA+AGG + Chr5:82786626-82786645 None:intergenic 30.0%
CTGATTGAATTTGTAGTGTT+TGG - Chr5:82785441-82785460 MsG0580028582.01.T01:intron 30.0%
CTTTATTGTGCACCATAATT+TGG + Chr5:82786452-82786471 None:intergenic 30.0%
GAAGAGAGAGTAATAGTTTA+TGG - Chr5:82785808-82785827 MsG0580028582.01.T01:intron 30.0%
GACGGAATTCATATACTTTA+GGG - Chr5:82785777-82785796 MsG0580028582.01.T01:intron 30.0%
TATGAGCAAGAATAAAGATG+GGG - Chr5:82786824-82786843 MsG0580028582.01.T01:intron 30.0%
TGAAATATGCATCAATCTTG+TGG - Chr5:82786693-82786712 MsG0580028582.01.T01:intron 30.0%
TGACGGAATTCATATACTTT+AGG - Chr5:82785776-82785795 MsG0580028582.01.T01:intron 30.0%
TGTTCATGTTAATGTGCTTA+AGG - Chr5:82786632-82786651 MsG0580028582.01.T01:intron 30.0%
TTCCAAAAATAAATTCCCCA+AGG - Chr5:82785848-82785867 MsG0580028582.01.T01:intron 30.0%
TTCCACAATATAAAGGAAAG+TGG + Chr5:82786191-82786210 None:intergenic 30.0%
TTCGAGATTTGTGAAACAAA+CGG - Chr5:82785219-82785238 MsG0580028582.01.T01:exon 30.0%
! AAATTGGAGACTTTGAGAAT+TGG - Chr5:82787011-82787030 MsG0580028582.01.T01:intron 30.0%
!! TTGTGGTTTGTTCCAAATTA+TGG - Chr5:82786437-82786456 MsG0580028582.01.T01:intron 30.0%
AAGGTTTGTGATCATGATCT+TGG + Chr5:82786251-82786270 None:intergenic 35.0%
ACCAAAGGCAATCATATTTG+AGG + Chr5:82785902-82785921 None:intergenic 35.0%
AGCTCATTTGAAAGTCTGTT+TGG + Chr5:82785651-82785670 None:intergenic 35.0%
CAAAAATAAATTCCCCAAGG+AGG - Chr5:82785851-82785870 MsG0580028582.01.T01:intron 35.0%
CAAATATGATTGCCTTTGGT+AGG - Chr5:82785902-82785921 MsG0580028582.01.T01:intron 35.0%
CACATATATACTCCTACCAA+AGG + Chr5:82785917-82785936 None:intergenic 35.0%
CATCAGTATAGTGTTCCAAT+TGG + Chr5:82786067-82786086 None:intergenic 35.0%
GACATAACATACTTCCATTC+AGG + Chr5:82786284-82786303 None:intergenic 35.0%
GTGAATTTCATCTAGAGAGA+TGG - Chr5:82785934-82785953 MsG0580028582.01.T01:intron 35.0%
TAATCTGTATTAGTCACGTC+AGG - Chr5:82786900-82786919 MsG0580028582.01.T01:intron 35.0%
TATAGTGTTCCAATTGGTAG+TGG + Chr5:82786061-82786080 None:intergenic 35.0%
TCAAGGATAATGCAAGAGTT+TGG - Chr5:82786666-82786685 MsG0580028582.01.T01:intron 35.0%
TGAGAATTGGATGAAGATCA+TGG - Chr5:82787024-82787043 MsG0580028582.01.T01:intron 35.0%
TGATGTTGATCTGACATAAG+AGG - Chr5:82786082-82786101 MsG0580028582.01.T01:intron 35.0%
TTGGAGAAGGAATCTAACTA+TGG + Chr5:82786345-82786364 None:intergenic 35.0%
TTTCAGAATGCTCAGATTGT+TGG - Chr5:82786004-82786023 MsG0580028582.01.T01:intron 35.0%
!! CCTTCTTTCTTGAGTTGATT+GGG - Chr5:82786486-82786505 MsG0580028582.01.T01:intron 35.0%
!! CTCCTTGGGGAATTTATTTT+TGG + Chr5:82785853-82785872 None:intergenic 35.0%
!! GTAGCCTTGTATCTTTATTC+TGG - Chr5:82787253-82787272 MsG0580028582.01.T01:CDS 35.0%
!! TATAGCGGATGAACTTATAG+AGG - Chr5:82785394-82785413 MsG0580028582.01.T01:intron 35.0%
!! TCCATCTTTTTGGCATCTAT+GGG - Chr5:82786136-82786155 MsG0580028582.01.T01:intron 35.0%
!!! ATAAGAGGCGTTTTCTTATG+TGG - Chr5:82786097-82786116 MsG0580028582.01.T01:intron 35.0%
!!! ATGGTTGTCATGCCATTTTT+GGG - Chr5:82786381-82786400 MsG0580028582.01.T01:intron 35.0%
!!! TAAGAGGCGTTTTCTTATGT+GGG - Chr5:82786098-82786117 MsG0580028582.01.T01:intron 35.0%
!!! TCATGCCATTTTTGGGTTAT+TGG - Chr5:82786388-82786407 MsG0580028582.01.T01:intron 35.0%
!!! TGCATCTTTCTTCGCTTTTT+CGG + Chr5:82785080-82785099 None:intergenic 35.0%
ACTTCATCACGAGCTTATAG+CGG + Chr5:82785553-82785572 None:intergenic 40.0%
CAAAAAGATGGAGAGTCATG+TGG + Chr5:82786128-82786147 None:intergenic 40.0%
CCCAATCAACTCAAGAAAGA+AGG + Chr5:82786489-82786508 None:intergenic 40.0%
GCAAGAATAAAGATGGGGAT+AGG - Chr5:82786829-82786848 MsG0580028582.01.T01:intron 40.0%
GCCTCAAATATGATTGCCTT+TGG - Chr5:82785898-82785917 MsG0580028582.01.T01:intron 40.0%
GGCACACTTACACTGATAAA+TGG - Chr5:82786714-82786733 MsG0580028582.01.T01:intron 40.0%
TGCTCATATGATTGTGCACA+AGG + Chr5:82786812-82786831 None:intergenic 40.0%
TTCTCTTAACACTGCTCTCA+AGG - Chr5:82785264-82785283 MsG0580028582.01.T01:CDS 40.0%
TTGTGAAACAAACGGATCAG+TGG - Chr5:82785227-82785246 MsG0580028582.01.T01:exon 40.0%
! CACATGACTCTCCATCTTTT+TGG - Chr5:82786126-82786145 MsG0580028582.01.T01:intron 40.0%
! GGCCACTTTCCTTTATATTG+TGG - Chr5:82786186-82786205 MsG0580028582.01.T01:intron 40.0%
! TTCTACTGCAGTTGCTTTGT+TGG - Chr5:82785876-82785895 MsG0580028582.01.T01:intron 40.0%
!! CCATTCAGGGCTTTAGAAAA+AGG + Chr5:82786270-82786289 None:intergenic 40.0%
!! CTCCATCTTTTTGGCATCTA+TGG - Chr5:82786135-82786154 MsG0580028582.01.T01:intron 40.0%
!! GCCTTCTTTCTTGAGTTGAT+TGG - Chr5:82786485-82786504 MsG0580028582.01.T01:intron 40.0%
!! TCTAATGATGTAGCACTGGT+TGG - Chr5:82787315-82787334 MsG0580028582.01.T01:CDS 40.0%
!!! AAGAGGCGTTTTCTTATGTG+GGG - Chr5:82786099-82786118 MsG0580028582.01.T01:intron 40.0%
!!! CCTTTTTCTAAAGCCCTGAA+TGG - Chr5:82786267-82786286 MsG0580028582.01.T01:intron 40.0%
!!! CTTCGCTTTTTCGGTTTGAT+TGG + Chr5:82785071-82785090 None:intergenic 40.0%
!!! GATGGTTGTCATGCCATTTT+TGG - Chr5:82786380-82786399 MsG0580028582.01.T01:intron 40.0%
AACACACGCGCAGAATCAAA+GGG - Chr5:82784986-82785005 MsG0580028582.01.T01:three_prime_UTR 45.0%
AAGAAAAGCAGAACGCGTCT+CGG - Chr5:82785102-82785121 MsG0580028582.01.T01:three_prime_UTR 45.0%
ACTGCAGTAGAAAACCTCCT+TGG + Chr5:82785868-82785887 None:intergenic 45.0%
CTGCAGTAGAAAACCTCCTT+GGG + Chr5:82785867-82785886 None:intergenic 45.0%
CTGTTAGTGGAAGCAGTGAA+TGG - Chr5:82785127-82785146 MsG0580028582.01.T01:three_prime_UTR 45.0%
GCCCATAGATGCCAAAAAGA+TGG + Chr5:82786140-82786159 None:intergenic 45.0%
GGCTTCTAATGATGTAGCAC+TGG - Chr5:82787311-82787330 MsG0580028582.01.T01:CDS 45.0%
TAACACACGCGCAGAATCAA+AGG - Chr5:82784985-82785004 MsG0580028582.01.T01:three_prime_UTR 45.0%
TGATCTTGGCTGCAACATCA+TGG + Chr5:82786237-82786256 None:intergenic 45.0%
TGCAGTAGAAAACCTCCTTG+GGG + Chr5:82785866-82785885 None:intergenic 45.0%
TTAGTGGAAGCAGTGAATGG+CGG - Chr5:82785130-82785149 MsG0580028582.01.T01:three_prime_UTR 45.0%
TTCACCAAGAGTGATCTTGC+AGG - Chr5:82787086-82787105 MsG0580028582.01.T01:CDS 45.0%
!! TGTAGCACTGGTTGGTGATT+TGG - Chr5:82787323-82787342 MsG0580028582.01.T01:CDS 45.0%
!!! AGAGGCGTTTTCTTATGTGG+GGG - Chr5:82786100-82786119 MsG0580028582.01.T01:intron 45.0%
ACGCGCAGAATCAAAGGGAT+TGG - Chr5:82784991-82785010 MsG0580028582.01.T01:three_prime_UTR 50.0%
CATCTAGAGAGATGGTTGCG+TGG - Chr5:82785942-82785961 MsG0580028582.01.T01:intron 50.0%
GCAACCTGCAAGATCACTCT+TGG + Chr5:82787093-82787112 None:intergenic 50.0%
GCGTCTACAACTGGACGTTT+GGG - Chr5:82785720-82785739 MsG0580028582.01.T01:intron 50.0%
! AGACCGTGTTGCTACTATCG+CGG - Chr5:82786165-82786184 MsG0580028582.01.T01:intron 50.0%
ACGCGTCTCGGATCTGTTAG+TGG - Chr5:82785114-82785133 MsG0580028582.01.T01:three_prime_UTR 55.0%
ACGTCCAGTTGTAGACGCGA+AGG + Chr5:82785718-82785737 None:intergenic 55.0%
CGCGTCTACAACTGGACGTT+TGG - Chr5:82785719-82785738 MsG0580028582.01.T01:intron 55.0%
CGTCCAGTTGTAGACGCGAA+GGG + Chr5:82785717-82785736 None:intergenic 55.0%
TAGCCCTTCGCGTCTACAAC+TGG - Chr5:82785711-82785730 MsG0580028582.01.T01:intron 55.0%
TGGCCGCGATAGTAGCAACA+CGG + Chr5:82786171-82786190 None:intergenic 55.0%
! GACGTTTGGGAGCGTGAACT+GGG - Chr5:82785733-82785752 MsG0580028582.01.T01:intron 55.0%
! GGACGTTTGGGAGCGTGAAC+TGG - Chr5:82785732-82785751 MsG0580028582.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 82784954 82787377 82784954 ID=MsG0580028582.01;Name=MsG0580028582.01
Chr5 mRNA 82784954 82787377 82784954 ID=MsG0580028582.01.T01;Parent=MsG0580028582.01;Name=MsG0580028582.01.T01;_AED=0.50;_eAED=0.50;_QI=14|1|1|1|1|1|2|278|135
Chr5 exon 82784954 82785321 82784954 ID=MsG0580028582.01.T01:exon:21333;Parent=MsG0580028582.01.T01
Chr5 exon 82787046 82787377 82787046 ID=MsG0580028582.01.T01:exon:21332;Parent=MsG0580028582.01.T01
Chr5 five_prime_UTR 82787364 82787377 82787364 ID=MsG0580028582.01.T01:five_prime_utr;Parent=MsG0580028582.01.T01
Chr5 CDS 82787046 82787363 82787046 ID=MsG0580028582.01.T01:cds;Parent=MsG0580028582.01.T01
Chr5 CDS 82785232 82785321 82785232 ID=MsG0580028582.01.T01:cds;Parent=MsG0580028582.01.T01
Chr5 three_prime_UTR 82784954 82785231 82784954 ID=MsG0580028582.01.T01:three_prime_utr;Parent=MsG0580028582.01.T01
Gene Sequence

>MsG0580028582.01.T01

ATGGAAGAACGCGATGTTAACACACGCGCAGAATCAAAGGGATTGGAAGATTCATTACAAAACCTAATTCACATTCATCATCAAAAATCTCTCATTCTCACCAATCAAACCGAAAAAGCGAAGAAAGATGCAATAAGAAAAGCAGAACGCGTCTCGGATCTGTTAGTGGAAGCAGTGAATGGCGGTGTTCAAGATTCGTTTATTTATCAGAAGAGAATTGAGCTTGAAATTCGTGCTCTTGCTGTAACTATTTCGAGATTTGTGAAACAAACGGATCAGTGGCTTAATGCTACTCATTCTCTTAACACTGCTCTCAAGGAAATTGGAGACTTTGAGAATTGGATGAAGATCATGGAATACGATTTTAAAAGTATTAATGCAGCTATACAAAACATTCACCAAGAGTGA

Protein sequence

>MsG0580028582.01.T01

MEERDVNTRAESKGLEDSLQNLIHIHHQKSLILTNQTEKAKKDAIRKAERVSDLLVEAVNGGVQDSFIYQKRIELEIRALAVTISRFVKQTDQWLNATHSLNTALKEIGDFENWMKIMEYDFKSINAAIQNIHQE*