AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044975.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044975.01.T01 MTR_8g062750 94.737 76 4 0 8 83 1 76 1.51e-50 154
MsG0880044975.01.T01 MTR_3g019310 94.737 76 4 0 8 83 1 76 1.90e-50 154
MsG0880044975.01.T01 MTR_3g019300 94.737 76 4 0 8 83 1 76 1.90e-50 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044975.01.T01 AT4G14342 86.842 76 10 0 8 83 1 76 1.80e-47 147
MsG0880044975.01.T01 AT4G14342 85.526 76 11 0 8 83 1 76 5.73e-47 145
MsG0880044975.01.T01 AT3G23325 84.211 76 12 0 8 83 1 76 2.69e-46 143

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGGCGTTGCGTTGCGTTGC+AGG 0.249732 8:-52315287 MsG0880044975.01.T01:CDS
ATCGCTCAAAACGAATCTAT+TGG 0.434978 8:-52315103 MsG0880044975.01.T01:CDS
TGGAAAAGTAAGAAAGTAAA+GGG 0.449644 8:+52315125 None:intergenic
CAAGCTAAATACGTTGGAAC+TGG 0.455380 8:-52315223 MsG0880044975.01.T01:CDS
AGCTTGAAGATGTTCAAGTT+GGG 0.460543 8:+52315240 None:intergenic
ATGGAAAAGTAAGAAAGTAA+AGG 0.463000 8:+52315124 None:intergenic
CTGATATCAACAGATTTGAA+TGG 0.473717 8:-52315195 MsG0880044975.01.T01:CDS
TAGCTTGAAGATGTTCAAGT+TGG 0.478107 8:+52315239 None:intergenic
AGTAAGAAAGTAAAGGGTAA+TGG 0.502423 8:+52315131 None:intergenic
GATAGCTATGCTTCATACAT+TGG 0.550538 8:-52315154 MsG0880044975.01.T01:CDS
AATTTAACGAAATCATGAAA+CGG 0.551069 8:-52315306 None:intergenic
TGATATCAACAGATTTGAAT+GGG 0.559675 8:-52315194 MsG0880044975.01.T01:CDS
CATCTTCAAGCTAAATACGT+TGG 0.585829 8:-52315229 MsG0880044975.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGGAAAAGTAAGAAAGTAA+AGG + Chr8:52315207-52315226 None:intergenic 25.0%
! TGATATCAACAGATTTGAAT+GGG - Chr8:52315134-52315153 MsG0880044975.01.T01:CDS 25.0%
! TGGAAAAGTAAGAAAGTAAA+GGG + Chr8:52315206-52315225 None:intergenic 25.0%
AGTAAGAAAGTAAAGGGTAA+TGG + Chr8:52315200-52315219 None:intergenic 30.0%
CTGATATCAACAGATTTGAA+TGG - Chr8:52315133-52315152 MsG0880044975.01.T01:CDS 30.0%
ATCGCTCAAAACGAATCTAT+TGG - Chr8:52315225-52315244 MsG0880044975.01.T01:CDS 35.0%
CATCTTCAAGCTAAATACGT+TGG - Chr8:52315099-52315118 MsG0880044975.01.T01:CDS 35.0%
GATAGCTATGCTTCATACAT+TGG - Chr8:52315174-52315193 MsG0880044975.01.T01:CDS 35.0%
TAGCTTGAAGATGTTCAAGT+TGG + Chr8:52315092-52315111 None:intergenic 35.0%
! AGCTTGAAGATGTTCAAGTT+GGG + Chr8:52315091-52315110 None:intergenic 35.0%
!! AATAGATTCGTTTTGAGCGA+TGG + Chr8:52315226-52315245 None:intergenic 35.0%
CAAGCTAAATACGTTGGAAC+TGG - Chr8:52315105-52315124 MsG0880044975.01.T01:CDS 40.0%
! ACAGCGTTACACTTTTATGC+AGG - Chr8:52315251-52315270 MsG0880044975.01.T01:CDS 40.0%
ACGGCGTTGCGTTGCGTTGC+AGG - Chr8:52315041-52315060 MsG0880044975.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 52315036 52315314 52315036 ID=MsG0880044975.01;Name=MsG0880044975.01
Chr8 mRNA 52315036 52315314 52315036 ID=MsG0880044975.01.T01;Parent=MsG0880044975.01;Name=MsG0880044975.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|92
Chr8 exon 52315036 52315314 52315036 ID=MsG0880044975.01.T01:exon:22390;Parent=MsG0880044975.01.T01
Chr8 CDS 52315036 52315314 52315036 ID=MsG0880044975.01.T01:cds;Parent=MsG0880044975.01.T01
Gene Sequence

>MsG0880044975.01.T01

ATGAAACGGCGTTGCGTTGCGTTGCAGGCGAGTGATAGATTTAACATAAATTCCCAACTTGAACATCTTCAAGCTAAATACGTTGGAACTGGACATGCTGATATCAACAGATTTGAATGGGCTGTGAATATTCAACGCGATAGCTATGCTTCATACATTGGCCATTACCCTTTACTTTCTTACTTTTCCATCGCTCAAAACGAATCTATTGGTAGACAGCGTTACACTTTTATGCAGGTAATTTTTCTCATGTTTATTGATTTCAGCTTAGTTAAGTGA

Protein sequence

>MsG0880044975.01.T01

MKRRCVALQASDRFNINSQLEHLQAKYVGTGHADINRFEWAVNIQRDSYASYIGHYPLLSYFSIAQNESIGRQRYTFMQVIFLMFIDFSLVK*