AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029932.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029932.01.T01 MTR_5g095050 85.147 579 30 3 1 538 1 564 0.0 920
MsG0580029932.01.T01 MTR_5g094950 53.627 386 132 7 1 364 1 361 4.37e-99 311
MsG0580029932.01.T01 MTR_5g094970 52.848 316 112 5 74 367 5 305 7.73e-79 251
MsG0580029932.01.T01 MTR_5g094960 36.089 496 195 11 1 448 1 422 1.25e-63 215
MsG0580029932.01.T01 MTR_5g094990 40.157 381 145 9 1 378 1 301 1.15e-54 191
MsG0580029932.01.T01 MTR_5g094850 39.737 380 145 7 3 375 1 303 1.55e-53 189
MsG0580029932.01.T01 MTR_5g094850 39.737 380 145 7 3 375 1 303 4.29e-53 188
MsG0580029932.01.T01 MTR_5g094920 37.656 401 156 9 1 354 1 354 1.47e-47 174
MsG0580029932.01.T01 MTR_5g077500 45.413 218 90 4 179 372 40 252 5.54e-37 138
MsG0580029932.01.T01 MTR_5g094910 41.256 223 100 6 154 354 30 243 9.00e-28 115
MsG0580029932.01.T01 MTR_5g095390 40.625 224 106 6 3 216 1 207 2.27e-26 110
MsG0580029932.01.T01 MTR_5g095370 35.196 179 82 4 1 176 1 148 1.37e-17 82.4
MsG0580029932.01.T01 MTR_1g071620 45.946 111 56 2 255 361 65 175 5.60e-17 80.5
MsG0580029932.01.T01 MTR_5g095400 40.000 135 64 3 35 166 5 125 1.17e-14 77.0
MsG0580029932.01.T01 MTR_5g095430 38.462 130 63 3 40 166 1 116 3.15e-13 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 124 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATATCAGCTAAGATATT+TGG 0.106981 5:+104148242 None:intergenic
TATTGTTAGTAACTTTATTT+TGG 0.155616 5:-104147543 MsG0580029932.01.T01:three_prime_UTR
AGTAATCAGTAACTTTATTT+TGG 0.175697 5:-104147624 MsG0580029932.01.T01:three_prime_UTR
GTCTCAACACTTCAAGTTCA+TGG 0.265620 5:+104149416 None:intergenic
GCTTAAATACTTACATTTGT+AGG 0.271720 5:+104148068 None:intergenic
GGAGAAACTCTTAAGGTTAA+AGG 0.271874 5:-104149653 MsG0580029932.01.T01:CDS
TGAAACAGTAAACAAGAATT+TGG 0.280388 5:-104148946 MsG0580029932.01.T01:CDS
AATTGTTAGTAACTTTGTTT+TGG 0.287134 5:-104147465 MsG0580029932.01.T01:three_prime_UTR
GCATGCATGGACCAATCTTT+TGG 0.292810 5:+104147735 None:intergenic
ATTTAAGCTCATCAATAGTT+TGG 0.295375 5:+104148791 None:intergenic
AGTTGGAGTGTGAGGTTAAT+AGG 0.300952 5:-104149297 MsG0580029932.01.T01:CDS
TTAATAAGTTGAAGGAAGAT+AGG 0.328036 5:-104149198 MsG0580029932.01.T01:CDS
GATTAATGAAGGAAATTTGA+TGG 0.341290 5:-104147419 MsG0580029932.01.T01:three_prime_UTR
GTCAAAATCATTACATGTTA+AGG 0.357681 5:-104147963 MsG0580029932.01.T01:CDS
AGCAATACTTGAACAAGATC+TGG 0.362926 5:+104149802 None:intergenic
GGGCTGCTTGCATCTTTGCC+TGG 0.376137 5:+104147833 None:intergenic
GTCCATGCATGCAGGCAATT+AGG 0.379329 5:-104147724 MsG0580029932.01.T01:three_prime_UTR
GGAAAGACAACAACTTGAAA+TGG 0.380275 5:-104149674 MsG0580029932.01.T01:CDS
TGAAAGAGTTGAAGGTGTTT+TGG 0.381818 5:-104149755 MsG0580029932.01.T01:CDS
GGTTGAAGTGTGAGGTTGAC+AGG 0.395371 5:-104149465 MsG0580029932.01.T01:CDS
ACATATCAGCTAAGATATTT+GGG 0.399117 5:+104148243 None:intergenic
CTTAGAGCAGAATGTTGAAA+TGG 0.410048 5:-104149695 MsG0580029932.01.T01:CDS
ATACTTACATTTGTAGGATC+AGG 0.410411 5:+104148074 None:intergenic
TGTAGCACCTTCTGCAAGTC+AGG 0.411433 5:-104148138 MsG0580029932.01.T01:CDS
ATGGAAACTCTTGACTTAGA+AGG 0.415536 5:-104149835 MsG0580029932.01.T01:CDS
GCAACTGGTGCATTTGCAAC+AGG 0.416299 5:+104148110 None:intergenic
TTGAAATGGAGAAACTCTTA+AGG 0.417844 5:-104149660 MsG0580029932.01.T01:CDS
TAATAGGTTGAAGGAAGATA+TGG 0.418849 5:-104149281 MsG0580029932.01.T01:CDS
GATCTTGTTCAAGTATTGCT+TGG 0.420517 5:-104149799 MsG0580029932.01.T01:CDS
TTGAAAACCAAGATGATTGC+TGG 0.423791 5:-104147857 MsG0580029932.01.T01:CDS
GCCTGGACCAGCAATCATCT+TGG 0.434652 5:+104147850 None:intergenic
AAGGAAGATAGGGTGGAGGA+TGG 0.446522 5:-104149187 MsG0580029932.01.T01:CDS
AAGTTATAATGCTCTCTTGA+AGG 0.449736 5:-104149572 MsG0580029932.01.T01:CDS
GAGTGATGAGCTAGAGTGTG+AGG 0.452371 5:-104149221 MsG0580029932.01.T01:CDS
TCAGCAGCCATAAAATCAAC+TGG 0.457108 5:+104148735 None:intergenic
TTTGGAGTTGACAAAGTTGA+AGG 0.457739 5:-104149030 MsG0580029932.01.T01:CDS
TTTGTAGGATCAGGCACATA+AGG 0.460936 5:+104148083 None:intergenic
TTGACATATTACTCGCGTCT+TGG 0.462570 5:-104148866 MsG0580029932.01.T01:CDS
AAAGTTAAAGATGCATCATC+AGG 0.464717 5:-104148681 MsG0580029932.01.T01:intron
AGTCAGGTCTAGTCATGTTA+AGG 0.465936 5:-104147924 MsG0580029932.01.T01:CDS
GAAACTTATGAAAGAGTTGA+AGG 0.466139 5:-104149763 MsG0580029932.01.T01:CDS
GTGTGAGGTTAATAGGTTGA+AGG 0.471254 5:-104149290 MsG0580029932.01.T01:CDS
GGCACATAAGGTATTGCAAC+TGG 0.471489 5:+104148095 None:intergenic
CACTGTCTTCAATTACCAAT+GGG 0.473098 5:+104147813 None:intergenic
GTGTGAGGTTAATAAGTTGA+AGG 0.473823 5:-104149206 MsG0580029932.01.T01:CDS
CTTGAACCTAAAATCGAACC+TGG 0.474651 5:-104149922 MsG0580029932.01.T01:CDS
TTAATAAGTTGAAAGAAGAA+AGG 0.475430 5:-104149366 MsG0580029932.01.T01:CDS
ACCAAGATGATTGCTGGTCC+AGG 0.475629 5:-104147851 MsG0580029932.01.T01:CDS
TCTATCACTAGACCTGACTT+TGG 0.479541 5:+104147889 None:intergenic
TTCTAAGAAGGAGAAACGTT+GGG 0.480244 5:-104149486 MsG0580029932.01.T01:CDS
AGCTTAAATGTCTCTGGTGG+TGG 0.480275 5:-104148777 MsG0580029932.01.T01:CDS
AAAGAAGAAAGGGTGGAGGA+TGG 0.483082 5:-104149355 MsG0580029932.01.T01:CDS
ATGTCAAGAAAGCCAAAGTC+AGG 0.490710 5:-104147940 MsG0580029932.01.T01:CDS
CCAGAGAGAGACCAAAAGAT+TGG 0.491518 5:-104147746 MsG0580029932.01.T01:three_prime_UTR
GTCCTAATTGCCTGCATGCA+TGG 0.492307 5:+104147722 None:intergenic
TCTGCTCTAAGAGTTGAATC+TGG 0.493051 5:+104149707 None:intergenic
AGACTTCATATGACATTAGC+AGG 0.496406 5:-104147694 MsG0580029932.01.T01:three_prime_UTR
CTTGCAGAAGGTGCTACAAT+TGG 0.498019 5:+104148143 None:intergenic
ATCAAGAATCACGTGGTTGG+AGG 0.498362 5:+104149891 None:intergenic
TTGATGAGCTTAAATGTCTC+TGG 0.501390 5:-104148783 MsG0580029932.01.T01:CDS
AACATGACTAGACCTGACTT+TGG 0.503870 5:+104147928 None:intergenic
AAGGAAGATATGGTGGAGGG+TGG 0.509218 5:-104149271 MsG0580029932.01.T01:CDS
TAATAAGTTGAAGGAAGATA+GGG 0.510480 5:-104149197 MsG0580029932.01.T01:CDS
GGTACTTCGACATGACCAAC+TGG 0.512667 5:+104148170 None:intergenic
ACTCTTAAGGTTAAAGGCTG+AGG 0.519219 5:-104149647 MsG0580029932.01.T01:CDS
TAATAAGTTGAAAGAAGAAA+GGG 0.520930 5:-104149365 MsG0580029932.01.T01:CDS
AGAGACGAAGATGATGAAGT+TGG 0.521296 5:-104149868 MsG0580029932.01.T01:CDS
GTTGAAGGAAGATAGGGTGG+AGG 0.521973 5:-104149191 MsG0580029932.01.T01:CDS
GTGGTATGTATGATTAATGA+AGG 0.522921 5:-104147430 MsG0580029932.01.T01:three_prime_UTR
AAGAAGAAAGGGTGGAGGAT+GGG 0.524336 5:-104149354 MsG0580029932.01.T01:CDS
TTTCTAAGAAGGAGAAACGT+TGG 0.528902 5:-104149487 MsG0580029932.01.T01:CDS
GGAAGAGTTAAAGAAAAGAG+AGG 0.532934 5:-104149626 MsG0580029932.01.T01:CDS
AGGAAGATAGGGTGGAGGAT+GGG 0.537473 5:-104149186 MsG0580029932.01.T01:CDS
TAGGTTGAAGGAAGATATGG+TGG 0.538319 5:-104149278 MsG0580029932.01.T01:CDS
TGGCAACTATAAGAGAATTG+AGG 0.540056 5:-104148926 MsG0580029932.01.T01:CDS
GAAGATTGTATTGTGTGGGC+TGG 0.542503 5:-104148996 MsG0580029932.01.T01:CDS
TAAGTTGAAGGAAGATAGGG+TGG 0.544598 5:-104149194 MsG0580029932.01.T01:CDS
TGAAGTTGGAGCATTTCCTA+TGG 0.545820 5:-104149854 MsG0580029932.01.T01:CDS
AACAATGCCACAATAGATGA+TGG 0.547079 5:+104147578 None:intergenic
TGGTTGGAGGAAGGTGACCC+AGG 0.547723 5:+104149904 None:intergenic
AGGAAGATATGGTGGAGGGT+GGG 0.550407 5:-104149270 MsG0580029932.01.T01:CDS
ATTGCTTGGATCTTTACAGA+TGG 0.550510 5:-104149785 MsG0580029932.01.T01:CDS
TCACTGTCTTCAATTACCAA+TGG 0.555333 5:+104147812 None:intergenic
GCAATACTTGAACAAGATCT+GGG 0.555962 5:+104149803 None:intergenic
AAAGATTGGTCCATGCATGC+AGG 0.559005 5:-104147732 MsG0580029932.01.T01:three_prime_UTR
CAAAGATGCAAGCAGCCCAT+TGG 0.565479 5:-104147828 MsG0580029932.01.T01:CDS
TCTATCAAGAATCACGTGGT+TGG 0.566169 5:+104149888 None:intergenic
TCTGGTGGTGGAAACAATGA+GGG 0.566395 5:-104148765 MsG0580029932.01.T01:CDS
TAAGTTGAAAGAAGAAAGGG+TGG 0.575684 5:-104149362 MsG0580029932.01.T01:CDS
CTCTGGTGGTGGAAACAATG+AGG 0.577778 5:-104148766 MsG0580029932.01.T01:CDS
AGTTGGTCATGTCGAAGTAC+CGG 0.578493 5:-104148168 MsG0580029932.01.T01:CDS
TATAATGCTCTCTTGAAGGA+AGG 0.584603 5:-104149568 MsG0580029932.01.T01:CDS
TTATGAACCATCATCTATTG+TGG 0.584836 5:-104147585 MsG0580029932.01.T01:three_prime_UTR
GAATGGTGAGTTGGAGTGTG+AGG 0.586676 5:-104149389 MsG0580029932.01.T01:CDS
GAGGGTGATGATATTGATGA+AGG 0.589257 5:-104147788 MsG0580029932.01.T01:CDS
GTTGAAAGAAGAAAGGGTGG+AGG 0.590291 5:-104149359 MsG0580029932.01.T01:CDS
AGAATCACGTGGTTGGAGGA+AGG 0.592347 5:+104149895 None:intergenic
GATGATATTGATGAAGGTGA+TGG 0.594476 5:-104147782 MsG0580029932.01.T01:CDS
GAAGGTGCTACAATTGGTGC+CGG 0.596125 5:+104148149 None:intergenic
TCTAAGTCAAGAGTTTCCAT+AGG 0.597975 5:+104149838 None:intergenic
GTTGAAGGAAGATATGGTGG+AGG 0.598207 5:-104149275 MsG0580029932.01.T01:CDS
CTGCATTGTTCTGCACCAGT+TGG 0.601057 5:-104148185 MsG0580029932.01.T01:CDS
GAGACAAAAGAATGGTGAGT+TGG 0.607745 5:-104149398 MsG0580029932.01.T01:CDS
TTGAAGGAAGATATGGTGGA+GGG 0.608134 5:-104149274 MsG0580029932.01.T01:CDS
TCTCTTGAAGGAAGGCAAGA+CGG 0.610347 5:-104149560 MsG0580029932.01.T01:CDS
GAATGATGAGTTGGAGTGTG+AGG 0.613027 5:-104149305 MsG0580029932.01.T01:CDS
GTAATTGAAGACAGTGATGA+GGG 0.618776 5:-104147806 MsG0580029932.01.T01:CDS
TAAGTTGACACAAGAAAGTG+AGG 0.628177 5:-104149521 MsG0580029932.01.T01:CDS
AAACGTTGGGTTGAAGTGTG+AGG 0.632328 5:-104149473 MsG0580029932.01.T01:CDS
GGAGGAAGATTGTATTGTGT+GGG 0.635950 5:-104149000 MsG0580029932.01.T01:CDS
GATACAGAGACCGCTCGAAG+TGG 0.636412 5:+104147496 None:intergenic
GGATCAACCTGACTTGCAGA+AGG 0.648405 5:+104148131 None:intergenic
CTGGTGGTGGAAACAATGAG+GGG 0.648857 5:-104148764 MsG0580029932.01.T01:CDS
GAAGTGTTGAGACAAAAGAA+TGG 0.649570 5:-104149406 MsG0580029932.01.T01:CDS
GGGAGGAAGATTGTATTGTG+TGG 0.661249 5:-104149001 MsG0580029932.01.T01:CDS
CGTCTCTATCAAGAATCACG+TGG 0.663157 5:+104149884 None:intergenic
GAATGATGAGTTGAAGTGTG+AGG 0.673142 5:-104149137 MsG0580029932.01.T01:intron
GGTAATTGAAGACAGTGATG+AGG 0.677445 5:-104147807 MsG0580029932.01.T01:CDS
TTGAACCTAAAATCGAACCT+GGG 0.681572 5:-104149921 MsG0580029932.01.T01:CDS
ATGAGCTTAAATGTCTCTGG+TGG 0.688563 5:-104148780 MsG0580029932.01.T01:CDS
GTGTTATGAACCACTTCGAG+CGG 0.691445 5:-104147506 MsG0580029932.01.T01:three_prime_UTR
CTTCATATGACATTAGCAGG+AGG 0.696178 5:-104147691 MsG0580029932.01.T01:three_prime_UTR
TGGTGGTGGAAACAATGAGG+GGG 0.698952 5:-104148763 MsG0580029932.01.T01:CDS
GCAGCCATAAAATCAACTGG+AGG 0.708098 5:+104148738 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATACAAACATTATACATA+AGG + Chr5:104148941-104148960 None:intergenic 15.0%
!! ATATAGTGTAAAGAAATTAT+AGG + Chr5:104148891-104148910 None:intergenic 15.0%
!! TATAGTGTAAAGAAATTATA+GGG + Chr5:104148890-104148909 None:intergenic 15.0%
!!! TATTGTTAGTAACTTTATTT+TGG - Chr5:104149793-104149812 MsG0580029932.01.T01:CDS 15.0%
!!! TTATACATTATTTTAGACTA+GGG - Chr5:104149056-104149075 MsG0580029932.01.T01:intron 15.0%
!!! TTTATACATTATTTTAGACT+AGG - Chr5:104149055-104149074 MsG0580029932.01.T01:intron 15.0%
!! ATGAAATCATATATATTCGA+AGG - Chr5:104148847-104148866 MsG0580029932.01.T01:CDS 20.0%
!! TATATATGATTTCATCAATG+AGG + Chr5:104148842-104148861 None:intergenic 20.0%
!!! AAACTATTGATTTTCTAAGA+AGG - Chr5:104147838-104147857 MsG0580029932.01.T01:CDS 20.0%
!!! AATTGTTAGTAACTTTGTTT+TGG - Chr5:104149871-104149890 MsG0580029932.01.T01:CDS 20.0%
!!! AGTAATCAGTAACTTTATTT+TGG - Chr5:104149712-104149731 MsG0580029932.01.T01:CDS 20.0%
!!! ATATTTTATTGTATCTCTAG+TGG - Chr5:104148975-104148994 MsG0580029932.01.T01:CDS 20.0%
!!! TAATAAGTTGAAAGAAGAAA+GGG - Chr5:104147971-104147990 MsG0580029932.01.T01:CDS 20.0%
!!! TATTTTATTGTATCTCTAGT+GGG - Chr5:104148976-104148995 MsG0580029932.01.T01:CDS 20.0%
!!! TGTAAGTTATGTATTTTACA+GGG - Chr5:104149338-104149357 MsG0580029932.01.T01:CDS 20.0%
!!! TTAATAAGTTGAAAGAAGAA+AGG - Chr5:104147970-104147989 MsG0580029932.01.T01:CDS 20.0%
! AACATATCAGCTAAGATATT+TGG + Chr5:104149097-104149116 None:intergenic 25.0%
! ACATATCAGCTAAGATATTT+GGG + Chr5:104149096-104149115 None:intergenic 25.0%
! ATTTAAGCTCATCAATAGTT+TGG + Chr5:104148548-104148567 None:intergenic 25.0%
! GATTAATGAAGGAAATTTGA+TGG - Chr5:104149917-104149936 MsG0580029932.01.T01:CDS 25.0%
! GCTTAAATACTTACATTTGT+AGG + Chr5:104149271-104149290 None:intergenic 25.0%
! GTCAAAATCATTACATGTTA+AGG - Chr5:104149373-104149392 MsG0580029932.01.T01:CDS 25.0%
! TGAAACAGTAAACAAGAATT+TGG - Chr5:104148390-104148409 MsG0580029932.01.T01:intron 25.0%
!! GTATTTTACAGGGAAATAAA+AGG - Chr5:104149348-104149367 MsG0580029932.01.T01:CDS 25.0%
!! GTGTAAGTTATGTATTTTAC+AGG - Chr5:104149337-104149356 MsG0580029932.01.T01:CDS 25.0%
!! TAATAAGTTGAAGGAAGATA+GGG - Chr5:104148139-104148158 MsG0580029932.01.T01:CDS 25.0%
!! TGGGTATTGTATTTCTATTA+GGG - Chr5:104148995-104149014 MsG0580029932.01.T01:CDS 25.0%
!! TTAATAAGTTGAAGGAAGAT+AGG - Chr5:104148138-104148157 MsG0580029932.01.T01:CDS 25.0%
AAAGTTAAAGATGCATCATC+AGG - Chr5:104148655-104148674 MsG0580029932.01.T01:intron 30.0%
AAATGTTTGGTTAGATTGCT+AGG - Chr5:104148710-104148729 MsG0580029932.01.T01:CDS 30.0%
AAGTTATAATGCTCTCTTGA+AGG - Chr5:104147764-104147783 MsG0580029932.01.T01:exon 30.0%
ACTACATGCTTCAATAAAAC+AGG + Chr5:104148764-104148783 None:intergenic 30.0%
ATACTTACATTTGTAGGATC+AGG + Chr5:104149265-104149284 None:intergenic 30.0%
GAAACTTATGAAAGAGTTGA+AGG - Chr5:104147573-104147592 MsG0580029932.01.T01:three_prime_UTR 30.0%
GACAAAGTTGAAGGAAAAAT+GGG - Chr5:104148315-104148334 MsG0580029932.01.T01:intron 30.0%
GAGAAAAAAGAATGATGAGT+TGG - Chr5:104148022-104148041 MsG0580029932.01.T01:intron 30.0%
GCTGGAATTGAGATTAAAAA+TGG - Chr5:104148358-104148377 MsG0580029932.01.T01:intron 30.0%
GGTATGGAATTGAATTCAAT+AGG - Chr5:104148676-104148695 MsG0580029932.01.T01:intron 30.0%
GGTGTGATCAAATAAATGTT+TGG - Chr5:104148697-104148716 MsG0580029932.01.T01:CDS 30.0%
GTGGTATGTATGATTAATGA+AGG - Chr5:104149906-104149925 MsG0580029932.01.T01:CDS 30.0%
TCGAAGGTTAAATTAGTAAG+TGG - Chr5:104148863-104148882 MsG0580029932.01.T01:CDS 30.0%
TCTTGCATGAAAAAGTTTCA+AGG - Chr5:104148509-104148528 MsG0580029932.01.T01:intron 30.0%
TGACAAAGTTGAAGGAAAAA+TGG - Chr5:104148314-104148333 MsG0580029932.01.T01:intron 30.0%
TTATGAACCATCATCTATTG+TGG - Chr5:104149751-104149770 MsG0580029932.01.T01:CDS 30.0%
TTGAAATGGAGAAACTCTTA+AGG - Chr5:104147676-104147695 MsG0580029932.01.T01:three_prime_UTR 30.0%
! GGGTATTGTATTTCTATTAG+GGG - Chr5:104148996-104149015 MsG0580029932.01.T01:CDS 30.0%
! GTGGGTATTGTATTTCTATT+AGG - Chr5:104148994-104149013 MsG0580029932.01.T01:CDS 30.0%
! TAATAGGTTGAAGGAAGATA+TGG - Chr5:104148055-104148074 MsG0580029932.01.T01:intron 30.0%
! TAATAGGTTGACGGAAAAAA+CGG - Chr5:104148223-104148242 MsG0580029932.01.T01:CDS 30.0%
!! ATAACTTGTTTGAACTTGTG+AGG - Chr5:104148796-104148815 MsG0580029932.01.T01:CDS 30.0%
!! GTTTTATTGAAGCATGTAGT+AGG - Chr5:104148764-104148783 MsG0580029932.01.T01:CDS 30.0%
AAAGTTGAAGGAAAAATGGG+AGG - Chr5:104148318-104148337 MsG0580029932.01.T01:intron 35.0%
AACAATGCCACAATAGATGA+TGG + Chr5:104149761-104149780 None:intergenic 35.0%
AGACTTCATATGACATTAGC+AGG - Chr5:104149642-104149661 MsG0580029932.01.T01:CDS 35.0%
AGCAATACTTGAACAAGATC+TGG + Chr5:104147537-104147556 None:intergenic 35.0%
AGTTGAAGTGTGAGGTTAAT+AGG - Chr5:104148207-104148226 MsG0580029932.01.T01:CDS 35.0%
ATGGAAACTCTTGACTTAGA+AGG - Chr5:104147501-104147520 MsG0580029932.01.T01:three_prime_UTR 35.0%
ATTGCTTGGATCTTTACAGA+TGG - Chr5:104147551-104147570 MsG0580029932.01.T01:three_prime_UTR 35.0%
CACTGTCTTCAATTACCAAT+GGG + Chr5:104149526-104149545 None:intergenic 35.0%
CTTAGAGCAGAATGTTGAAA+TGG - Chr5:104147641-104147660 MsG0580029932.01.T01:three_prime_UTR 35.0%
GATGATATTGATGAAGGTGA+TGG - Chr5:104149554-104149573 MsG0580029932.01.T01:CDS 35.0%
GCAATACTTGAACAAGATCT+GGG + Chr5:104147536-104147555 None:intergenic 35.0%
GGAAAGACAACAACTTGAAA+TGG - Chr5:104147662-104147681 MsG0580029932.01.T01:three_prime_UTR 35.0%
GGAAGAGTTAAAGAAAAGAG+AGG - Chr5:104147710-104147729 MsG0580029932.01.T01:three_prime_UTR 35.0%
GGAGAAACTCTTAAGGTTAA+AGG - Chr5:104147683-104147702 MsG0580029932.01.T01:three_prime_UTR 35.0%
GGGGTTGTATCATATTATGT+AGG - Chr5:104149015-104149034 MsG0580029932.01.T01:CDS 35.0%
GTAATTGAAGACAGTGATGA+GGG - Chr5:104149530-104149549 MsG0580029932.01.T01:CDS 35.0%
TAAAGATGCATCATCAGGTA+TGG - Chr5:104148660-104148679 MsG0580029932.01.T01:intron 35.0%
TATAATGCTCTCTTGAAGGA+AGG - Chr5:104147768-104147787 MsG0580029932.01.T01:exon 35.0%
TCACTGTCTTCAATTACCAA+TGG + Chr5:104149527-104149546 None:intergenic 35.0%
TCTAAGTCAAGAGTTTCCAT+AGG + Chr5:104147501-104147520 None:intergenic 35.0%
TGGCAACTATAAGAGAATTG+AGG - Chr5:104148410-104148429 MsG0580029932.01.T01:intron 35.0%
TTGAAAACCAAGATGATTGC+TGG - Chr5:104149479-104149498 MsG0580029932.01.T01:CDS 35.0%
TTGAACCTAAAATCGAACCT+GGG - Chr5:104147415-104147434 MsG0580029932.01.T01:three_prime_UTR 35.0%
TTGATGAGCTTAAATGTCTC+TGG - Chr5:104148553-104148572 MsG0580029932.01.T01:intron 35.0%
! GATCTTGTTCAAGTATTGCT+TGG - Chr5:104147537-104147556 MsG0580029932.01.T01:three_prime_UTR 35.0%
! GTGTGAGGTTAATAAGTTGA+AGG - Chr5:104147962-104147981 MsG0580029932.01.T01:CDS 35.0%
! TAAGTTGAAAGAAGAAAGGG+TGG - Chr5:104147974-104147993 MsG0580029932.01.T01:CDS 35.0%
! TAAGTTGACACAAGAAAGTG+AGG - Chr5:104147815-104147834 MsG0580029932.01.T01:CDS 35.0%
! TGACAGGTTGAACGAAAAAA+GGG - Chr5:104147887-104147906 MsG0580029932.01.T01:CDS 35.0%
! TGAGATGGAGAATAAGGTTT+TGG - Chr5:104148288-104148307 MsG0580029932.01.T01:intron 35.0%
! TTCTAAGAAGGAGAAACGTT+GGG - Chr5:104147850-104147869 MsG0580029932.01.T01:CDS 35.0%
! TTGACAGGTTGAACGAAAAA+AGG - Chr5:104147886-104147905 MsG0580029932.01.T01:CDS 35.0%
! TTTCTAAGAAGGAGAAACGT+TGG - Chr5:104147849-104147868 MsG0580029932.01.T01:CDS 35.0%
! TTTGGAGTTGACAAAGTTGA+AGG - Chr5:104148306-104148325 MsG0580029932.01.T01:intron 35.0%
!! GAAGTGTTGAGACAAAAGAA+TGG - Chr5:104147930-104147949 MsG0580029932.01.T01:CDS 35.0%
!! GTTTTGGATCTAAACACTAG+TGG - Chr5:104149887-104149906 MsG0580029932.01.T01:CDS 35.0%
!!! TGAAAGAGTTGAAGGTGTTT+TGG - Chr5:104147581-104147600 MsG0580029932.01.T01:three_prime_UTR 35.0%
AAACAGGAAAATCACGTTCC+AGG + Chr5:104148748-104148767 None:intergenic 40.0%
AACATGACTAGACCTGACTT+TGG + Chr5:104149411-104149430 None:intergenic 40.0%
ACTCTTAAGGTTAAAGGCTG+AGG - Chr5:104147689-104147708 MsG0580029932.01.T01:three_prime_UTR 40.0%
ACTTGTGAGGCTACTAGTAA+TGG - Chr5:104148809-104148828 MsG0580029932.01.T01:CDS 40.0%
AGAGAAAATGACTGGTGAGA+TGG - Chr5:104148273-104148292 MsG0580029932.01.T01:intron 40.0%
AGAGACGAAGATGATGAAGT+TGG - Chr5:104147468-104147487 MsG0580029932.01.T01:three_prime_UTR 40.0%
AGTCAGGTCTAGTCATGTTA+AGG - Chr5:104149412-104149431 MsG0580029932.01.T01:CDS 40.0%
AGTTGGAGTGTGAGGTTAAT+AGG - Chr5:104147955-104147974 MsG0580029932.01.T01:CDS 40.0%
ATGAGCTTAAATGTCTCTGG+TGG - Chr5:104148556-104148575 MsG0580029932.01.T01:intron 40.0%
ATGTCAAGAAAGCCAAAGTC+AGG - Chr5:104149396-104149415 MsG0580029932.01.T01:CDS 40.0%
ATGTCGCAAAAACCAAAGTC+AGG - Chr5:104149435-104149454 MsG0580029932.01.T01:CDS 40.0%
CTTCATATGACATTAGCAGG+AGG - Chr5:104149645-104149664 MsG0580029932.01.T01:CDS 40.0%
CTTGAACCTAAAATCGAACC+TGG - Chr5:104147414-104147433 MsG0580029932.01.T01:three_prime_UTR 40.0%
GAGACAAAAGAATGGTGAGT+TGG - Chr5:104147938-104147957 MsG0580029932.01.T01:CDS 40.0%
GAGGGTGATGATATTGATGA+AGG - Chr5:104149548-104149567 MsG0580029932.01.T01:CDS 40.0%
GCATGAAAAAGTTTCAAGGC+TGG - Chr5:104148513-104148532 MsG0580029932.01.T01:intron 40.0%
GGAGGAAGATTGTATTGTGT+GGG - Chr5:104148336-104148355 MsG0580029932.01.T01:intron 40.0%
GGTAATTGAAGACAGTGATG+AGG - Chr5:104149529-104149548 MsG0580029932.01.T01:CDS 40.0%
GTTTGGTTAGATTGCTAGGT+AGG - Chr5:104148714-104148733 MsG0580029932.01.T01:CDS 40.0%
TCAGCAGCCATAAAATCAAC+TGG + Chr5:104148604-104148623 None:intergenic 40.0%
TCTATCAAGAATCACGTGGT+TGG + Chr5:104147451-104147470 None:intergenic 40.0%
TCTATCACTAGACCTGACTT+TGG + Chr5:104149450-104149469 None:intergenic 40.0%
TGAAAAAGTTTCAAGGCTGG+AGG - Chr5:104148516-104148535 MsG0580029932.01.T01:intron 40.0%
TTGACATATTACTCGCGTCT+TGG - Chr5:104148470-104148489 MsG0580029932.01.T01:intron 40.0%
TTTGTAGGATCAGGCACATA+AGG + Chr5:104149256-104149275 None:intergenic 40.0%
! GAATGATGAGTTGAAGTGTG+AGG - Chr5:104148199-104148218 MsG0580029932.01.T01:CDS 40.0%
! GTCTCAACACTTCAAGTTCA+TGG + Chr5:104147923-104147942 None:intergenic 40.0%
! GTGTGAGGTTAATAGGTTGA+AGG - Chr5:104148046-104148065 MsG0580029932.01.T01:intron 40.0%
! GTGTGAGGTTAATAGGTTGA+CGG - Chr5:104148046-104148065 MsG0580029932.01.T01:intron 40.0%
! TAAGTTGAAGGAAGATAGGG+TGG - Chr5:104148142-104148161 MsG0580029932.01.T01:CDS 40.0%
! TAGGTTGAAGGAAGATATGG+TGG - Chr5:104148058-104148077 MsG0580029932.01.T01:intron 40.0%
! TGAAGTTGGAGCATTTCCTA+TGG - Chr5:104147482-104147501 MsG0580029932.01.T01:three_prime_UTR 40.0%
! TTGAAGGAAGATATGGTGGA+GGG - Chr5:104148062-104148081 MsG0580029932.01.T01:intron 40.0%
!! GGATCCTCCAGTTGATTTTA+TGG - Chr5:104148594-104148613 MsG0580029932.01.T01:intron 40.0%
!! GTTTTGGTGAGAAGGGATTT+GGG - Chr5:104147597-104147616 MsG0580029932.01.T01:three_prime_UTR 40.0%
!! TCTGCTCTAAGAGTTGAATC+TGG + Chr5:104147632-104147651 None:intergenic 40.0%
!!! CAGGTTCGATTTTAGGTTCA+AGG + Chr5:104147416-104147435 None:intergenic 40.0%
!!! TGAAGGTGTTTTGGTGAGAA+GGG - Chr5:104147590-104147609 MsG0580029932.01.T01:three_prime_UTR 40.0%
!!! TGTTTTGGTGAGAAGGGATT+TGG - Chr5:104147596-104147615 MsG0580029932.01.T01:three_prime_UTR 40.0%
!!! TTGAAGGTGTTTTGGTGAGA+AGG - Chr5:104147589-104147608 MsG0580029932.01.T01:three_prime_UTR 40.0%
AAAGAAGAAAGGGTGGAGGA+TGG - Chr5:104147981-104148000 MsG0580029932.01.T01:intron 45.0%
AACGAAAAAAGGGAGGAGGA+AGG - Chr5:104147897-104147916 MsG0580029932.01.T01:CDS 45.0%
AAGAAGAAAGGGTGGAGGAT+GGG - Chr5:104147982-104148001 MsG0580029932.01.T01:intron 45.0%
AGATTGCTAGGTAGGTAGCT+TGG - Chr5:104148722-104148741 MsG0580029932.01.T01:CDS 45.0%
AGCTTAAATGTCTCTGGTGG+TGG - Chr5:104148559-104148578 MsG0580029932.01.T01:intron 45.0%
AGTTGGTCATGTCGAAGTAC+CGG - Chr5:104149168-104149187 MsG0580029932.01.T01:CDS 45.0%
ATCAAGAATCACGTGGTTGG+AGG + Chr5:104147448-104147467 None:intergenic 45.0%
ATTCACTCACACACACACAC+AGG + Chr5:104149299-104149318 None:intergenic 45.0%
CCAGAGAGAGACCAAAAGAT+TGG - Chr5:104149590-104149609 MsG0580029932.01.T01:CDS 45.0%
CGTCTCTATCAAGAATCACG+TGG + Chr5:104147455-104147474 None:intergenic 45.0%
GAAGATTGTATTGTGTGGGC+TGG - Chr5:104148340-104148359 MsG0580029932.01.T01:intron 45.0%
GACTGGTGAGATGGAGAATA+AGG - Chr5:104148282-104148301 MsG0580029932.01.T01:intron 45.0%
GCAGCCATAAAATCAACTGG+AGG + Chr5:104148601-104148620 None:intergenic 45.0%
GGGAGGAAGATTGTATTGTG+TGG - Chr5:104148335-104148354 MsG0580029932.01.T01:intron 45.0%
GTGTTATGAACCACTTCGAG+CGG - Chr5:104149830-104149849 MsG0580029932.01.T01:CDS 45.0%
GTTGAAAGAAGAAAGGGTGG+AGG - Chr5:104147977-104147996 MsG0580029932.01.T01:CDS 45.0%
GTTGAACGAAAAAAGGGAGG+AGG - Chr5:104147893-104147912 MsG0580029932.01.T01:CDS 45.0%
GTTGAAGGAAGATATGGTGG+AGG - Chr5:104148061-104148080 MsG0580029932.01.T01:intron 45.0%
TCTCTTGAAGGAAGGCAAGA+CGG - Chr5:104147776-104147795 MsG0580029932.01.T01:CDS 45.0%
TGGTGTGCAGAGAAAATGAC+TGG - Chr5:104148265-104148284 MsG0580029932.01.T01:intron 45.0%
! AAAGATTGGTCCATGCATGC+AGG - Chr5:104149604-104149623 MsG0580029932.01.T01:CDS 45.0%
! CAGGTTGAACGAAAAAAGGG+AGG - Chr5:104147890-104147909 MsG0580029932.01.T01:CDS 45.0%
! CCAATCTTTTGGTCTCTCTC+TGG + Chr5:104149593-104149612 None:intergenic 45.0%
! GAATGATGAGTTGGAGTGTG+AGG - Chr5:104148031-104148050 MsG0580029932.01.T01:intron 45.0%
! GCATGCATGGACCAATCTTT+TGG + Chr5:104149604-104149623 None:intergenic 45.0%
! TAGGTTGACGGAAAAAACGG+CGG - Chr5:104148226-104148245 MsG0580029932.01.T01:CDS 45.0%
!! AAACGTTGGGTTGAAGTGTG+AGG - Chr5:104147863-104147882 MsG0580029932.01.T01:CDS 45.0%
!! CTTGCAGAAGGTGCTACAAT+TGG + Chr5:104149196-104149215 None:intergenic 45.0%
!! GGCACATAAGGTATTGCAAC+TGG + Chr5:104149244-104149263 None:intergenic 45.0%
!! TCTGGTGGTGGAAACAATGA+GGG - Chr5:104148571-104148590 MsG0580029932.01.T01:intron 45.0%
AAGGAAGATAGGGTGGAGGA+TGG - Chr5:104148149-104148168 MsG0580029932.01.T01:CDS 50.0%
ACCAAGATGATTGCTGGTCC+AGG - Chr5:104149485-104149504 MsG0580029932.01.T01:CDS 50.0%
ACGGAAAAAACGGCGGATGA+TGG - Chr5:104148233-104148252 MsG0580029932.01.T01:CDS 50.0%
AGAATCACGTGGTTGGAGGA+AGG + Chr5:104147444-104147463 None:intergenic 50.0%
AGGAAGATAGGGTGGAGGAT+GGG - Chr5:104148150-104148169 MsG0580029932.01.T01:CDS 50.0%
CAAAGATGCAAGCAGCCCAT+TGG - Chr5:104149508-104149527 MsG0580029932.01.T01:CDS 50.0%
CTGCATTGTTCTGCACCAGT+TGG - Chr5:104149151-104149170 MsG0580029932.01.T01:CDS 50.0%
GAGTGATGAGCTAGAGTGTG+AGG - Chr5:104148115-104148134 MsG0580029932.01.T01:CDS 50.0%
GCAACTGGTGCATTTGCAAC+AGG + Chr5:104149229-104149248 None:intergenic 50.0%
GGTACTTCGACATGACCAAC+TGG + Chr5:104149169-104149188 None:intergenic 50.0%
GGTTGAAGTGTGAGGTTGAC+AGG - Chr5:104147871-104147890 MsG0580029932.01.T01:CDS 50.0%
GTCCATGCATGCAGGCAATT+AGG - Chr5:104149612-104149631 MsG0580029932.01.T01:CDS 50.0%
GTCCTAATTGCCTGCATGCA+TGG + Chr5:104149617-104149636 None:intergenic 50.0%
GTTGAAGGAAGATAGGGTGG+AGG - Chr5:104148145-104148164 MsG0580029932.01.T01:CDS 50.0%
TGTAGCACCTTCTGCAAGTC+AGG - Chr5:104149198-104149217 MsG0580029932.01.T01:CDS 50.0%
! AAGGAAGATATGGTGGAGGG+TGG - Chr5:104148065-104148084 MsG0580029932.01.T01:intron 50.0%
! AGGAAGATATGGTGGAGGGT+GGG - Chr5:104148066-104148085 MsG0580029932.01.T01:intron 50.0%
! GAATGGTGAGTTGGAGTGTG+AGG - Chr5:104147947-104147966 MsG0580029932.01.T01:CDS 50.0%
! GGATCAACCTGACTTGCAGA+AGG + Chr5:104149208-104149227 None:intergenic 50.0%
! GGTGACCCAGGTTCGATTTT+AGG + Chr5:104147423-104147442 None:intergenic 50.0%
!! CTCTGGTGGTGGAAACAATG+AGG - Chr5:104148570-104148589 MsG0580029932.01.T01:intron 50.0%
!! CTGGTGGTGGAAACAATGAG+GGG - Chr5:104148572-104148591 MsG0580029932.01.T01:intron 50.0%
!! GAAGGTGCTACAATTGGTGC+CGG + Chr5:104149190-104149209 None:intergenic 50.0%
!! TGGTGGTGGAAACAATGAGG+GGG - Chr5:104148573-104148592 MsG0580029932.01.T01:intron 50.0%
GATACAGAGACCGCTCGAAG+TGG + Chr5:104149843-104149862 None:intergenic 55.0%
GCCTGGACCAGCAATCATCT+TGG + Chr5:104149489-104149508 None:intergenic 55.0%
GCTAGGTAGGTAGCTTGGCC+TGG - Chr5:104148727-104148746 MsG0580029932.01.T01:CDS 60.0%
GGGCTGCTTGCATCTTTGCC+TGG + Chr5:104149506-104149525 None:intergenic 60.0%
TGGTTGGAGGAAGGTGACCC+AGG + Chr5:104147435-104147454 None:intergenic 60.0%
! GCGGATGATGGCTGTGAGCT+TGG - Chr5:104148245-104148264 MsG0580029932.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 104147408 104149950 104147408 ID=MsG0580029932.01;Name=MsG0580029932.01
Chr5 mRNA 104147408 104149950 104147408 ID=MsG0580029932.01.T01;Parent=MsG0580029932.01;Name=MsG0580029932.01.T01;_AED=0.16;_eAED=0.19;_QI=0|0.33|0.25|1|1|1|4|361|538
Chr5 exon 104147408 104147999 104147408 ID=MsG0580029932.01.T01:exon:35397;Parent=MsG0580029932.01.T01
Chr5 exon 104148082 104148281 104148082 ID=MsG0580029932.01.T01:exon:35396;Parent=MsG0580029932.01.T01
Chr5 exon 104148682 104149054 104148682 ID=MsG0580029932.01.T01:exon:35395;Parent=MsG0580029932.01.T01
Chr5 exon 104149138 104149950 104149138 ID=MsG0580029932.01.T01:exon:35394;Parent=MsG0580029932.01.T01
Chr5 CDS 104149138 104149950 104149138 ID=MsG0580029932.01.T01:cds;Parent=MsG0580029932.01.T01
Chr5 CDS 104148682 104149054 104148682 ID=MsG0580029932.01.T01:cds;Parent=MsG0580029932.01.T01
Chr5 CDS 104148082 104148281 104148082 ID=MsG0580029932.01.T01:cds;Parent=MsG0580029932.01.T01
Chr5 CDS 104147769 104147999 104147769 ID=MsG0580029932.01.T01:cds;Parent=MsG0580029932.01.T01
Chr5 three_prime_UTR 104147408 104147768 104147408 ID=MsG0580029932.01.T01:three_prime_utr;Parent=MsG0580029932.01.T01
Gene Sequence

>MsG0580029932.01.T01

ATGAACCTTGAACCTAAAATCGAACCTGGGTCACCTTCCTCCAACCACGTGATTCTTGATAGAGACGAAGATGATGAAGTTGGAGCATTTCCTATGGAAACTCTTGACTTAGAAGGAATGACTATCCCAGATCTTGTTCAAGTATTGCTTGGATCTTTACAGATGGAAACTTATGAAAGAGTTGAAGGTGTTTTGGTGAGAAGGGATTTGGGACTGAGAGACCAGATTCAACTCTTAGAGCAGAATGTTGAAATGGAAAGACAACAACTTGAAATGGAGAAACTCTTAAGGTTAAAGGCTGAGGAAGAGTTAAAGAAAAGAGAGGAAATTTGTCAGAAAATGAAGAAAGTGCAAGAAAGTTATAATGCTCTCTTGAAGGAAGGCAAGACGGCTGAAAAAACTCATAATAAGTTGACACAAGAAAGTGAGGAAACTATTGATTTTCTAAGAAGGAGAAACGTTGGGTTGAAGTGTGAGGTTGACAGGTTGAACGAAAAAAGGGAGGAGGAAGGCCATGAACTTGAAGTGTTGAGACAAAAGAATGGTGAGTTGGAGTGTGAGGTTAATAAGTTGAAAGAAGAAAGGGTGGAGGATGGGAACAAATTTGATGTGTTGAGAAAAAAGAATGATGAGTTGGAGTGTGAGGTTAATAGGTTGAAGGAAGATATGGTGGAGGGTGGGAACAAATTTGAAGTGTTAAGAAAGAAGAGTGATGAGCTAGAGTGTGAGGTTAATAAGTTGAAGGAAGATAGGGTGGAGGATGGGAACAAATTTGAAGTTCTAAGAAAAAAGAATGATGAGTTGAAGTGTGAGGTTTTGGAGTTGACAAAGTTGAAGGAAAAATGGGAGGAAGATTGTATTGTGTGGGCTGGAATTGAGATTAAAAATGGTGAATTGAATGAAACAGTAAACAAGAATTTGGCAACTATAAGAGAATTGAGGAACGAAAATAGCAAATTAGTAAATGATAAGCACAATGTTGACATATTACTCGCGTCTTGGATCACGAAGTTTAGAGTCTTGCATGAAAAAGTTTCAAGGCTGGAGGATGATTCCAAACTATTGATGAGCTTAAATGTCTCTGGTGGTGGAAACAATGAGGGGGATCCTCCAGTTGATTTTATGGCTGCTGATTCTGAAGAGAAAGACAAAGAGAAAGAGTCTAAAGTTAAAGATGCATCATCAGGGTGTTATTTAATTGACCCAAATATCTTAGCTGATATGTTTGAAGATGATGTGCTTGAAATGTTATCTCAAAAACTGCATTGTTCTGCACCAGTTGGTCATGTCGAAGTACCGGCACCAATTGTAGCACCTTCTGCAAGTCAGGTTGATCCTGTTGCAAATGCACCAGTTGCAATACCTTATGTGCCTGATCCTACAAATGGAAATAAAAGGAAGTCAAAATCATTACATGTTAAGGATGTCAAGAAAGCCAAAGTCAGGTCTAGTCATGTTAAGGATGTCGCAAAAACCAAAGTCAGGTCTAGTGATAGACTTAGAAAATTGAAAACCAAGATGATTGCTGGTCCAGGCAAAGATGCAAGCAGCCCATTGGTAATTGAAGACAGTGATGAGGGTGATGATATTGATGAAGGTGATGGTATTGATGATTAA
Protein sequence

>MsG0580029932.01.T01

MNLEPKIEPGSPSSNHVILDRDEDDEVGAFPMETLDLEGMTIPDLVQVLLGSLQMETYERVEGVLVRRDLGLRDQIQLLEQNVEMERQQLEMEKLLRLKAEEELKKREEICQKMKKVQESYNALLKEGKTAEKTHNKLTQESEETIDFLRRRNVGLKCEVDRLNEKREEEGHELEVLRQKNGELECEVNKLKEERVEDGNKFDVLRKKNDELECEVNRLKEDMVEGGNKFEVLRKKSDELECEVNKLKEDRVEDGNKFEVLRKKNDELKCEVLELTKLKEKWEEDCIVWAGIEIKNGELNETVNKNLATIRELRNENSKLVNDKHNVDILLASWITKFRVLHEKVSRLEDDSKLLMSLNVSGGGNNEGDPPVDFMAADSEEKDKEKESKVKDASSGCYLIDPNILADMFEDDVLEMLSQKLHCSAPVGHVEVPAPIVAPSASQVDPVANAPVAIPYVPDPTNGNKRKSKSLHVKDVKKAKVRSSHVKDVAKTKVRSSDRLRKLKTKMIAGPGKDASSPLVIEDSDEGDDIDEGDGIDD*