AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047460.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047460.01.T01 MTR_4g127870 96.512 172 6 0 1 172 1 172 6.85e-127 353
MsG0880047460.01.T01 MTR_6g018300 51.220 164 76 3 7 169 16 176 1.71e-61 188
MsG0880047460.01.T01 MTR_6g018310 50.000 168 80 3 3 169 15 179 1.80e-61 188
MsG0880047460.01.T01 MTR_7g007120 51.282 156 72 3 15 169 28 180 1.08e-58 181
MsG0880047460.01.T01 MTR_7g007120 51.948 154 70 3 15 167 28 178 1.44e-58 181
MsG0880047460.01.T01 MTR_7g007220 51.948 154 70 3 15 167 28 178 1.66e-58 181
MsG0880047460.01.T01 MTR_7g007120 51.948 154 70 3 15 167 28 178 1.81e-58 181
MsG0880047460.01.T01 MTR_7g007200 51.948 154 70 3 15 167 28 178 2.18e-58 181
MsG0880047460.01.T01 MTR_7g007230 51.299 154 71 3 15 167 28 178 2.33e-57 178
MsG0880047460.01.T01 MTR_7g007210 52.857 140 62 3 15 153 28 164 1.62e-52 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047460.01.T01 AT1G67090 50.000 172 82 3 1 171 11 179 8.04e-62 189
MsG0880047460.01.T01 AT5G38410 52.121 165 75 3 8 171 18 179 8.96e-62 189
MsG0880047460.01.T01 AT5G38420 52.727 165 74 3 8 171 18 179 9.06e-62 189
MsG0880047460.01.T01 AT5G38430 52.121 165 75 3 8 171 18 179 2.32e-61 188
MsG0880047460.01.T01 AT5G38430 52.121 165 75 3 8 171 18 179 2.32e-61 188
MsG0880047460.01.T01 AT5G38410 50.000 170 76 3 8 171 18 184 2.81e-60 186
MsG0880047460.01.T01 AT5G38410 51.220 164 71 4 8 171 18 172 2.80e-58 180
MsG0880047460.01.T01 AT1G67090 39.640 111 64 2 1 111 11 118 3.60e-22 87.0

Find 53 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATTTCCTATTATTAA+TGG 0.204312 8:+86664699 None:intergenic
ATCAGAAAGGGGTGGTAAAT+AGG 0.291264 8:+86664669 None:intergenic
TTAGTGGCATATGAATAAAA+TGG 0.309190 8:-86663739 MsG0880047460.01.T01:three_prime_UTR
ATGGGAGGTATTGGACATTA+TGG 0.311655 8:-86664014 MsG0880047460.01.T01:CDS
TCAAATTTCCTATTATTAAT+GGG 0.315443 8:+86664700 None:intergenic
CATGGAATCCCATTAATAAT+AGG 0.318513 8:-86664708 MsG0880047460.01.T01:CDS
GTTGCTGGCCCTGGCTTTGT+GGG 0.327668 8:-86664961 MsG0880047460.01.T01:CDS
GCAGCTTCAGTTGCTGGCCC+TGG 0.329745 8:-86664970 MsG0880047460.01.T01:CDS
GCATATGAATAAAATGGATT+TGG 0.334280 8:-86663733 MsG0880047460.01.T01:three_prime_UTR
AGTCCAACTCATCAAATCTT+TGG 0.338452 8:-86664933 MsG0880047460.01.T01:CDS
GAAGAGAAATAAGAACAAAA+TGG 0.341169 8:+86663811 None:intergenic
ACATTTGCAGCTTCAGTTGC+TGG 0.350554 8:-86664976 MsG0880047460.01.T01:CDS
AAGCATCTTATATATGCATT+TGG 0.378298 8:+86663919 None:intergenic
TAAGTAGTTGTTGTGGTAGT+AGG 0.382906 8:+86663844 None:intergenic
TTCAATGGAATAAGTAGTAC+TGG 0.385537 8:+86664909 None:intergenic
AGTTGCTGGCCCTGGCTTTG+TGG 0.386009 8:-86664962 MsG0880047460.01.T01:CDS
GACTTCAAGCCCACAAAGCC+AGG 0.391842 8:+86664952 None:intergenic
GATACTATGATGGGAGGTAT+TGG 0.398812 8:-86664023 MsG0880047460.01.T01:CDS
GTACTACTTATTCCATTGAA+TGG 0.406489 8:-86664906 MsG0880047460.01.T01:CDS
TTATGGAAGCTACCAATGTT+TGG 0.409185 8:-86663997 MsG0880047460.01.T01:CDS
ATTAGTGTATTAATATATCT+TGG 0.413683 8:-86663668 MsG0880047460.01.T01:three_prime_UTR
GTAGCGTAGTACTTGTTTAG+TGG 0.420260 8:-86663755 MsG0880047460.01.T01:three_prime_UTR
AATAAATGTCTGATCTTTGA+AGG 0.426750 8:+86665020 None:intergenic
TGCAATTGAGTCATCAGAAA+GGG 0.441885 8:+86664657 None:intergenic
GACTAACCTCATCAAATTCA+AGG 0.447817 8:+86664593 None:intergenic
CATCATAGTATCCTGGCATC+TGG 0.482846 8:+86664034 None:intergenic
GCAGTGAATCTTAGAACCAT+TGG 0.489711 8:+86664867 None:intergenic
CAGATGCCAGGATACTATGA+TGG 0.514747 8:-86664033 MsG0880047460.01.T01:CDS
GGCAGAATAGTTGAGATGAA+AGG 0.524279 8:+86665044 None:intergenic
GGGCCAAAGATTTGATGAGT+TGG 0.537281 8:+86664930 None:intergenic
ATAAAATGGATTTGGAAAGT+CGG 0.537618 8:-86663725 MsG0880047460.01.T01:three_prime_UTR
GATTGACTACATGCTCAAGA+AGG 0.539499 8:-86664629 MsG0880047460.01.T01:CDS
TACCTCCCATCATAGTATCC+TGG 0.541106 8:+86664027 None:intergenic
TTCTGATGACTCAATTGCAA+AGG 0.545642 8:-86664653 MsG0880047460.01.T01:CDS
TTTGTGAATAATAAAAGCCA+TGG 0.556871 8:+86663867 None:intergenic
TTCTAAGATTCACTGCATGA+AGG 0.557279 8:-86664860 MsG0880047460.01.T01:intron
TTGCAATTGAGTCATCAGAA+AGG 0.572127 8:+86664656 None:intergenic
AGAGCAAATAGCCAGATGCC+AGG 0.574689 8:-86664045 MsG0880047460.01.T01:CDS
ACCTCATCAAATTCAAGGCA+AGG 0.584505 8:+86664598 None:intergenic
AGATGCCAGGATACTATGAT+GGG 0.593649 8:-86664032 MsG0880047460.01.T01:CDS
CAAGCGACACATGCAAGCCA+TGG 0.600771 8:-86663884 MsG0880047460.01.T01:CDS
ATTGACTACATGCTCAAGAA+GGG 0.603348 8:-86664628 MsG0880047460.01.T01:CDS
ACCTTGCCTTGAATTTGATG+AGG 0.604745 8:-86664599 MsG0880047460.01.T01:intron
AAGACTGAGAATTTCAACAA+AGG 0.606526 8:+86663784 None:intergenic
ACTACATGCTCAAGAAGGGA+TGG 0.608538 8:-86664624 MsG0880047460.01.T01:CDS
AATTAATTAAGTAGTTGTTG+TGG 0.614403 8:+86663837 None:intergenic
ACTTCAAGCCCACAAAGCCA+GGG 0.614433 8:+86664953 None:intergenic
GCAATTGAGTCATCAGAAAG+GGG 0.629169 8:+86664658 None:intergenic
TCAATGGAATAAGTAGTACT+GGG 0.635347 8:+86664910 None:intergenic
AAATGTCTGATCTTTGAAGG+AGG 0.660147 8:+86665023 None:intergenic
TGCCAGGATACTATGATGGG+AGG 0.662604 8:-86664029 MsG0880047460.01.T01:CDS
GAGTCGTTACAACCAAACAT+TGG 0.684048 8:+86663985 None:intergenic
ATTGAGTCATCAGAAAGGGG+TGG 0.697277 8:+86664661 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATATCTTATTAATAATA+TGG - Chr8:86664566-86664585 MsG0880047460.01.T01:intron 10.0%
!! TTATATTATAAACTAGATTT+AGG + Chr8:86664110-86664129 None:intergenic 10.0%
!!! GTAGTTTTTTTATTATTATA+AGG - Chr8:86664131-86664150 MsG0880047460.01.T01:intron 10.0%
!!! TAAATCTAGTTTATAATATA+AGG - Chr8:86664109-86664128 MsG0880047460.01.T01:intron 10.0%
!! ATTAGTGTATTAATATATCT+TGG - Chr8:86665006-86665025 MsG0880047460.01.T01:exon 15.0%
!! ATTATAAGGAAAAAACAATT+AGG - Chr8:86664145-86664164 MsG0880047460.01.T01:intron 15.0%
!! GATTCATATAATATAGATTA+AGG - Chr8:86664198-86664217 MsG0880047460.01.T01:intron 15.0%
!! TCAAATTTCCTATTATTAAT+GGG + Chr8:86663977-86663996 None:intergenic 15.0%
!! TTCAAATTTCCTATTATTAA+TGG + Chr8:86663978-86663997 None:intergenic 15.0%
!!! AAGTTATACAAAAGTTTTAT+TGG + Chr8:86664484-86664503 None:intergenic 15.0%
!! AATTAATTAAGTAGTTGTTG+TGG + Chr8:86664840-86664859 None:intergenic 20.0%
!! ATTATAAACTAGATTTAGGT+TGG + Chr8:86664106-86664125 None:intergenic 20.0%
!! TAGAAAACAAAAACATCAAA+AGG + Chr8:86664541-86664560 None:intergenic 20.0%
!!! CTTTAATTTATTCTATGAAG+TGG - Chr8:86664501-86664520 MsG0880047460.01.T01:intron 20.0%
! AACCATGCTATCATAATATA+TGG + Chr8:86663861-86663880 None:intergenic 25.0%
! AAGCATCTTATATATGCATT+TGG + Chr8:86664758-86664777 None:intergenic 25.0%
! AATAAATGTCTGATCTTTGA+AGG + Chr8:86663657-86663676 None:intergenic 25.0%
! AATATGGAACTTGTATTGTT+TGG - Chr8:86664582-86664601 MsG0880047460.01.T01:intron 25.0%
! ACATATATGTACTAGTATCT+TGG - Chr8:86663881-86663900 MsG0880047460.01.T01:CDS 25.0%
! ATATAGATTAAGGCAATGTT+TGG - Chr8:86664208-86664227 MsG0880047460.01.T01:intron 25.0%
! ATTTGATTAAAAATGTCTGC+AGG - Chr8:86663674-86663693 MsG0880047460.01.T01:three_prime_UTR 25.0%
! GAAGAGAAATAAGAACAAAA+TGG + Chr8:86664866-86664885 None:intergenic 25.0%
! TTAGTGGCATATGAATAAAA+TGG - Chr8:86664935-86664954 MsG0880047460.01.T01:CDS 25.0%
! TTTGTAACTTAAAACAGACA+TGG - Chr8:86663948-86663967 MsG0880047460.01.T01:CDS 25.0%
! TTTGTGAATAATAAAAGCCA+TGG + Chr8:86664810-86664829 None:intergenic 25.0%
!! ATAAAATGGATTTGGAAAGT+CGG - Chr8:86664949-86664968 MsG0880047460.01.T01:CDS 25.0%
!! GCATATGAATAAAATGGATT+TGG - Chr8:86664941-86664960 MsG0880047460.01.T01:CDS 25.0%
AAGACTGAGAATTTCAACAA+AGG + Chr8:86664893-86664912 None:intergenic 30.0%
AGCCATATATTATGATAGCA+TGG - Chr8:86663856-86663875 MsG0880047460.01.T01:CDS 30.0%
ATTGTTTGGAAATGGAAGTT+TGG - Chr8:86664596-86664615 MsG0880047460.01.T01:intron 30.0%
CATGGAATCCCATTAATAAT+AGG - Chr8:86663966-86663985 MsG0880047460.01.T01:CDS 30.0%
GAACTTGTATTGTTTGGAAA+TGG - Chr8:86664588-86664607 MsG0880047460.01.T01:intron 30.0%
GTACTACTTATTCCATTGAA+TGG - Chr8:86663768-86663787 MsG0880047460.01.T01:three_prime_UTR 30.0%
TCAATGGAATAAGTAGTACT+GGG + Chr8:86663767-86663786 None:intergenic 30.0%
TTCAATGGAATAAGTAGTAC+TGG + Chr8:86663768-86663787 None:intergenic 30.0%
! AGTCTTCTTTTTCCATTCAA+TGG + Chr8:86663783-86663802 None:intergenic 30.0%
AAAAAGAAGACTGTCTCCAA+TGG - Chr8:86663791-86663810 MsG0880047460.01.T01:three_prime_UTR 35.0%
AGTCCAACTCATCAAATCTT+TGG - Chr8:86663741-86663760 MsG0880047460.01.T01:three_prime_UTR 35.0%
ATTGACTACATGCTCAAGAA+GGG - Chr8:86664046-86664065 MsG0880047460.01.T01:CDS 35.0%
GACTAACCTCATCAAATTCA+AGG + Chr8:86664084-86664103 None:intergenic 35.0%
TAAGTAGTTGTTGTGGTAGT+AGG + Chr8:86664833-86664852 None:intergenic 35.0%
TGCAATTGAGTCATCAGAAA+GGG + Chr8:86664020-86664039 None:intergenic 35.0%
TTATGGAAGCTACCAATGTT+TGG - Chr8:86664677-86664696 MsG0880047460.01.T01:CDS 35.0%
TTCTGATGACTCAATTGCAA+AGG - Chr8:86664021-86664040 MsG0880047460.01.T01:CDS 35.0%
TTGCAATTGAGTCATCAGAA+AGG + Chr8:86664021-86664040 None:intergenic 35.0%
! AAATGTCTGATCTTTGAAGG+AGG + Chr8:86663654-86663673 None:intergenic 35.0%
!! TTCTAAGATTCACTGCATGA+AGG - Chr8:86663814-86663833 MsG0880047460.01.T01:three_prime_UTR 35.0%
ACCTCATCAAATTCAAGGCA+AGG + Chr8:86664079-86664098 None:intergenic 40.0%
ACCTTGCCTTGAATTTGATG+AGG - Chr8:86664075-86664094 MsG0880047460.01.T01:intron 40.0%
AGATGCCAGGATACTATGAT+GGG - Chr8:86664642-86664661 MsG0880047460.01.T01:CDS 40.0%
ATCAGAAAGGGGTGGTAAAT+AGG + Chr8:86664008-86664027 None:intergenic 40.0%
GAGTCGTTACAACCAAACAT+TGG + Chr8:86664692-86664711 None:intergenic 40.0%
GATTGACTACATGCTCAAGA+AGG - Chr8:86664045-86664064 MsG0880047460.01.T01:CDS 40.0%
GCAATTGAGTCATCAGAAAG+GGG + Chr8:86664019-86664038 None:intergenic 40.0%
GCAGTGAATCTTAGAACCAT+TGG + Chr8:86663810-86663829 None:intergenic 40.0%
GGCAGAATAGTTGAGATGAA+AGG + Chr8:86663633-86663652 None:intergenic 40.0%
GTAGCGTAGTACTTGTTTAG+TGG - Chr8:86664919-86664938 MsG0880047460.01.T01:CDS 40.0%
! GATACTATGATGGGAGGTAT+TGG - Chr8:86664651-86664670 MsG0880047460.01.T01:CDS 40.0%
!! ATGGGAGGTATTGGACATTA+TGG - Chr8:86664660-86664679 MsG0880047460.01.T01:CDS 40.0%
ACATTTGCAGCTTCAGTTGC+TGG - Chr8:86663698-86663717 MsG0880047460.01.T01:three_prime_UTR 45.0%
ACTACATGCTCAAGAAGGGA+TGG - Chr8:86664050-86664069 MsG0880047460.01.T01:CDS 45.0%
ATTGAGTCATCAGAAAGGGG+TGG + Chr8:86664016-86664035 None:intergenic 45.0%
CAGATGCCAGGATACTATGA+TGG - Chr8:86664641-86664660 MsG0880047460.01.T01:CDS 45.0%
CATCATAGTATCCTGGCATC+TGG + Chr8:86664643-86664662 None:intergenic 45.0%
GGGCCAAAGATTTGATGAGT+TGG + Chr8:86663747-86663766 None:intergenic 45.0%
TACCTCCCATCATAGTATCC+TGG + Chr8:86664650-86664669 None:intergenic 45.0%
!!! TAAACATTATTTAATTTTAA+TGG - Chr8:86665038-86665057 MsG0880047460.01.T01:five_prime_UTR 5.0%
ACTTCAAGCCCACAAAGCCA+GGG + Chr8:86663724-86663743 None:intergenic 50.0%
AGAGCAAATAGCCAGATGCC+AGG - Chr8:86664629-86664648 MsG0880047460.01.T01:CDS 50.0%
! TGCCAGGATACTATGATGGG+AGG - Chr8:86664645-86664664 MsG0880047460.01.T01:CDS 50.0%
CAAGCGACACATGCAAGCCA+TGG - Chr8:86664790-86664809 MsG0880047460.01.T01:intron 55.0%
GACTTCAAGCCCACAAAGCC+AGG + Chr8:86663725-86663744 None:intergenic 55.0%
!! AGTTGCTGGCCCTGGCTTTG+TGG - Chr8:86663712-86663731 MsG0880047460.01.T01:three_prime_UTR 60.0%
!! GTTGCTGGCCCTGGCTTTGT+GGG - Chr8:86663713-86663732 MsG0880047460.01.T01:three_prime_UTR 60.0%
GCAGCTTCAGTTGCTGGCCC+TGG - Chr8:86663704-86663723 MsG0880047460.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 86663626 86665070 86663626 ID=MsG0880047460.01;Name=MsG0880047460.01
Chr8 mRNA 86663626 86665070 86663626 ID=MsG0880047460.01.T01;Parent=MsG0880047460.01;Name=MsG0880047460.01.T01;_AED=0.46;_eAED=0.46;_QI=60|1|1|1|1|1|3|217|173
Chr8 exon 86663626 86664082 86663626 ID=MsG0880047460.01.T01:exon:17939;Parent=MsG0880047460.01.T01
Chr8 exon 86664600 86664731 86664600 ID=MsG0880047460.01.T01:exon:17938;Parent=MsG0880047460.01.T01
Chr8 exon 86664861 86665070 86664861 ID=MsG0880047460.01.T01:exon:17937;Parent=MsG0880047460.01.T01
Chr8 five_prime_UTR 86665011 86665070 86665011 ID=MsG0880047460.01.T01:five_prime_utr;Parent=MsG0880047460.01.T01
Chr8 CDS 86664861 86665010 86664861 ID=MsG0880047460.01.T01:cds;Parent=MsG0880047460.01.T01
Chr8 CDS 86664600 86664731 86664600 ID=MsG0880047460.01.T01:cds;Parent=MsG0880047460.01.T01
Chr8 CDS 86663843 86664082 86663843 ID=MsG0880047460.01.T01:cds;Parent=MsG0880047460.01.T01
Chr8 three_prime_UTR 86663626 86663842 86663626 ID=MsG0880047460.01.T01:three_prime_utr;Parent=MsG0880047460.01.T01
Gene Sequence

>MsG0880047460.01.T01

ATGTCTGCAGGAACATTTGCAGCTTCAGTTGCTGGCCCTGGCTTTGTGGGCTTGAAGTCCAACTCATCAAATCTTTGGCCCAGTACTACTTATTCCATTGAATGGAAAAAGAAGACTGTCTCCAATGGTTCTAAGATTCACTGCATGAAGACATGGAATCCCATTAATAATAGGAAATTTGAAACACTTTCCTATTTACCACCCCTTTCTGATGACTCAATTGCAAAGGAGATTGACTACATGCTCAAGAAGGGATGGACACCTTGCCTTGAATTTGATGAGTTTGGATGTATTCATAGAGCAAATAGCCAGATGCCAGGATACTATGATGGGAGGTATTGGACATTATGGAAGCTACCAATGTTTGGTTGTAACGACTCTTCACAAGTGCTTAATGAGATTAATGAATGCAAAAAGACATATCCAAATGCATATATAAGATGCTTAGCATTTGACAACAAGCGACACATGCAAGCCATGGCTTTTATTATTCACAAACCTACTACCACAACAACTACTTAA

Protein sequence

>MsG0880047460.01.T01

MSAGTFAASVAGPGFVGLKSNSSNLWPSTTYSIEWKKKTVSNGSKIHCMKTWNPINNRKFETLSYLPPLSDDSIAKEIDYMLKKGWTPCLEFDEFGCIHRANSQMPGYYDGRYWTLWKLPMFGCNDSSQVLNEINECKKTYPNAYIRCLAFDNKRHMQAMAFIIHKPTTTTTT*