Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036557.01.T01 | GAU19864.1 | 77.801 | 482 | 102 | 3 | 11 | 490 | 1 | 479 | 0 | 774 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036557.01.T01 | Q9LJB4 | 38.153 | 498 | 237 | 14 | 11 | 487 | 1 | 448 | 4.71E-88 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036557.01.T01 | A0A2Z6LPH5 | 77.801 | 482 | 102 | 3 | 11 | 490 | 1 | 479 | 0.0 | 774 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036557.01.T01 | MTR_7g013850 | 79.959 | 484 | 92 | 4 | 11 | 490 | 1 | 483 | 0.0 | 770 |
| MsG0780036557.01.T01 | MTR_7g014160 | 58.455 | 479 | 182 | 11 | 15 | 487 | 6 | 473 | 0.0 | 538 |
| MsG0780036557.01.T01 | MTR_7g014200 | 59.298 | 484 | 174 | 12 | 11 | 487 | 1 | 468 | 0.0 | 538 |
| MsG0780036557.01.T01 | MTR_7g014610 | 58.799 | 483 | 185 | 9 | 11 | 487 | 1 | 475 | 0.0 | 519 |
| MsG0780036557.01.T01 | MTR_7g014620 | 52.711 | 461 | 182 | 7 | 29 | 487 | 6 | 432 | 4.33e-149 | 433 |
| MsG0780036557.01.T01 | MTR_7g014630 | 57.143 | 301 | 116 | 7 | 16 | 311 | 7 | 299 | 2.05e-109 | 329 |
| MsG0780036557.01.T01 | MTR_6g015315 | 39.149 | 470 | 262 | 10 | 19 | 487 | 6 | 452 | 4.57e-103 | 316 |
| MsG0780036557.01.T01 | MTR_7g014330 | 39.749 | 478 | 255 | 15 | 19 | 487 | 3 | 456 | 8.33e-101 | 310 |
| MsG0780036557.01.T01 | MTR_6g015320 | 36.983 | 484 | 268 | 12 | 11 | 487 | 1 | 454 | 7.59e-100 | 308 |
| MsG0780036557.01.T01 | MTR_6g015830 | 39.794 | 485 | 243 | 14 | 19 | 487 | 9 | 460 | 2.19e-99 | 307 |
| MsG0780036557.01.T01 | MTR_6g015850 | 39.104 | 491 | 251 | 16 | 11 | 487 | 1 | 457 | 2.41e-99 | 306 |
| MsG0780036557.01.T01 | MTR_7g014230 | 38.413 | 479 | 264 | 12 | 19 | 489 | 5 | 460 | 1.06e-98 | 305 |
| MsG0780036557.01.T01 | MTR_7g014250 | 38.994 | 477 | 260 | 14 | 19 | 487 | 3 | 456 | 3.37e-98 | 303 |
| MsG0780036557.01.T01 | MTR_6g015815 | 40.700 | 457 | 240 | 11 | 35 | 487 | 31 | 460 | 4.59e-97 | 301 |
| MsG0780036557.01.T01 | MTR_7g014180 | 70.391 | 179 | 46 | 5 | 11 | 188 | 1 | 173 | 1.20e-73 | 230 |
| MsG0780036557.01.T01 | MTR_3g028210 | 35.258 | 485 | 271 | 16 | 18 | 487 | 8 | 464 | 1.22e-73 | 240 |
| MsG0780036557.01.T01 | MTR_7g014670 | 56.477 | 193 | 82 | 2 | 299 | 490 | 71 | 262 | 9.52e-69 | 221 |
| MsG0780036557.01.T01 | MTR_2g089765 | 32.311 | 489 | 282 | 15 | 21 | 487 | 21 | 482 | 1.11e-65 | 220 |
| MsG0780036557.01.T01 | MTR_7g014360 | 33.907 | 407 | 219 | 12 | 88 | 487 | 23 | 386 | 1.64e-64 | 214 |
| MsG0780036557.01.T01 | MTR_2g089670 | 31.965 | 463 | 276 | 14 | 36 | 487 | 43 | 477 | 7.39e-60 | 204 |
| MsG0780036557.01.T01 | MTR_2g089650 | 28.514 | 498 | 300 | 15 | 11 | 487 | 1 | 463 | 2.91e-57 | 197 |
| MsG0780036557.01.T01 | MTR_2g089735 | 31.390 | 446 | 272 | 11 | 52 | 487 | 40 | 461 | 3.06e-57 | 197 |
| MsG0780036557.01.T01 | MTR_3g087640 | 29.376 | 497 | 289 | 11 | 19 | 487 | 10 | 472 | 9.43e-57 | 196 |
| MsG0780036557.01.T01 | MTR_7g015860 | 30.629 | 493 | 282 | 17 | 18 | 487 | 5 | 460 | 1.72e-54 | 189 |
| MsG0780036557.01.T01 | MTR_2g089755 | 28.541 | 466 | 290 | 13 | 38 | 487 | 37 | 475 | 5.25e-52 | 183 |
| MsG0780036557.01.T01 | MTR_8g012750 | 30.786 | 471 | 281 | 15 | 36 | 485 | 24 | 470 | 1.44e-51 | 182 |
| MsG0780036557.01.T01 | MTR_6g015310 | 30.789 | 393 | 201 | 11 | 73 | 465 | 12 | 333 | 8.19e-49 | 171 |
| MsG0780036557.01.T01 | MTR_7g014310 | 29.429 | 333 | 166 | 9 | 140 | 470 | 41 | 306 | 6.56e-39 | 144 |
| MsG0780036557.01.T01 | MTR_2g089665 | 24.945 | 457 | 202 | 14 | 21 | 464 | 7 | 335 | 4.20e-24 | 103 |
| MsG0780036557.01.T01 | MTR_3g435300 | 29.286 | 280 | 166 | 9 | 19 | 286 | 4 | 263 | 6.17e-23 | 98.6 |
| MsG0780036557.01.T01 | MTR_4g007540 | 24.330 | 485 | 285 | 22 | 18 | 480 | 3 | 427 | 7.78e-22 | 98.2 |
| MsG0780036557.01.T01 | MTR_8g075610 | 24.439 | 401 | 232 | 16 | 54 | 439 | 30 | 374 | 3.08e-21 | 96.3 |
| MsG0780036557.01.T01 | MTR_3g070170 | 25.721 | 451 | 270 | 17 | 18 | 443 | 12 | 422 | 5.45e-20 | 92.8 |
| MsG0780036557.01.T01 | MTR_3g049150 | 24.601 | 439 | 267 | 18 | 73 | 488 | 51 | 448 | 8.33e-19 | 89.4 |
| MsG0780036557.01.T01 | MTR_7g086000 | 24.395 | 496 | 309 | 19 | 17 | 487 | 3 | 457 | 5.52e-18 | 86.7 |
| MsG0780036557.01.T01 | MTR_2g064495 | 24.622 | 463 | 271 | 23 | 42 | 485 | 28 | 431 | 1.08e-17 | 85.5 |
| MsG0780036557.01.T01 | MTR_7g014210 | 38.318 | 107 | 64 | 2 | 345 | 449 | 24 | 130 | 1.78e-17 | 80.1 |
| MsG0780036557.01.T01 | MTR_2g105330 | 24.946 | 461 | 272 | 23 | 42 | 485 | 28 | 431 | 4.31e-17 | 84.0 |
| MsG0780036557.01.T01 | MTR_7g014205 | 37.615 | 109 | 56 | 2 | 342 | 449 | 17 | 114 | 1.04e-16 | 76.6 |
| MsG0780036557.01.T01 | MTR_3g026950 | 23.448 | 435 | 270 | 17 | 74 | 483 | 51 | 447 | 1.21e-16 | 82.4 |
| MsG0780036557.01.T01 | MTR_3g088420 | 23.188 | 483 | 263 | 15 | 27 | 477 | 14 | 420 | 5.16e-16 | 80.5 |
| MsG0780036557.01.T01 | MTR_4g127650 | 23.441 | 465 | 267 | 17 | 54 | 485 | 46 | 454 | 7.63e-16 | 80.1 |
| MsG0780036557.01.T01 | MTR_4g022800 | 22.601 | 469 | 292 | 16 | 27 | 477 | 14 | 429 | 7.65e-16 | 80.1 |
| MsG0780036557.01.T01 | MTR_8g096530 | 24.599 | 374 | 233 | 10 | 71 | 439 | 53 | 382 | 8.68e-16 | 79.7 |
| MsG0780036557.01.T01 | MTR_4g022720 | 22.766 | 470 | 283 | 17 | 27 | 477 | 14 | 422 | 1.87e-15 | 79.0 |
| MsG0780036557.01.T01 | MTR_4g097090 | 23.061 | 477 | 290 | 19 | 36 | 485 | 28 | 454 | 7.34e-15 | 77.0 |
| MsG0780036557.01.T01 | MTR_7g085990 | 23.841 | 302 | 199 | 8 | 60 | 359 | 42 | 314 | 3.02e-14 | 75.1 |
| MsG0780036557.01.T01 | MTR_7g085930 | 23.988 | 346 | 230 | 11 | 16 | 359 | 4 | 318 | 3.78e-14 | 75.1 |
| MsG0780036557.01.T01 | MTR_3g101970 | 24.434 | 442 | 260 | 20 | 18 | 443 | 6 | 389 | 4.18e-14 | 74.7 |
| MsG0780036557.01.T01 | MTR_7g014340 | 54.930 | 71 | 25 | 2 | 19 | 85 | 3 | 70 | 1.79e-13 | 66.6 |
| MsG0780036557.01.T01 | MTR_8g079970 | 23.158 | 475 | 276 | 18 | 27 | 480 | 15 | 421 | 4.80e-12 | 68.2 |
| MsG0780036557.01.T01 | MTR_4g022825 | 21.348 | 445 | 264 | 15 | 38 | 456 | 24 | 408 | 1.04e-11 | 67.4 |
| MsG0780036557.01.T01 | MTR_8g467580 | 25.641 | 429 | 245 | 21 | 27 | 439 | 15 | 385 | 1.39e-11 | 67.0 |
| MsG0780036557.01.T01 | MTR_8g079950 | 23.899 | 477 | 270 | 17 | 27 | 480 | 15 | 421 | 1.54e-11 | 66.6 |
| MsG0780036557.01.T01 | MTR_7g014220 | 55.357 | 56 | 22 | 1 | 32 | 87 | 12 | 64 | 1.89e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036557.01.T01 | AT3G29590 | 38.153 | 498 | 237 | 14 | 11 | 487 | 1 | 448 | 4.80e-89 | 280 |
| MsG0780036557.01.T01 | AT3G29670 | 38.750 | 480 | 253 | 13 | 18 | 488 | 3 | 450 | 7.17e-88 | 277 |
| MsG0780036557.01.T01 | AT5G39080 | 38.266 | 473 | 264 | 14 | 11 | 477 | 1 | 451 | 2.91e-87 | 275 |
| MsG0780036557.01.T01 | AT3G29635 | 37.810 | 484 | 255 | 15 | 18 | 487 | 3 | 454 | 7.13e-87 | 274 |
| MsG0780036557.01.T01 | AT5G39050 | 37.113 | 485 | 267 | 14 | 16 | 487 | 8 | 467 | 1.54e-86 | 274 |
| MsG0780036557.01.T01 | AT5G39090 | 36.842 | 475 | 264 | 14 | 16 | 487 | 6 | 447 | 4.64e-84 | 267 |
| MsG0780036557.01.T01 | AT1G03940 | 31.313 | 495 | 275 | 12 | 19 | 487 | 9 | 464 | 6.50e-70 | 231 |
| MsG0780036557.01.T01 | AT5G61160 | 33.615 | 473 | 278 | 14 | 18 | 483 | 3 | 446 | 1.51e-68 | 226 |
| MsG0780036557.01.T01 | AT3G29680 | 34.454 | 476 | 268 | 14 | 16 | 483 | 4 | 443 | 7.97e-68 | 224 |
| MsG0780036557.01.T01 | AT1G03495 | 30.914 | 372 | 216 | 11 | 11 | 370 | 1 | 343 | 8.04e-44 | 158 |
| MsG0780036557.01.T01 | AT2G19070 | 23.902 | 410 | 240 | 14 | 54 | 438 | 40 | 402 | 1.66e-20 | 94.4 |
| MsG0780036557.01.T01 | AT5G48930 | 24.066 | 482 | 300 | 18 | 18 | 485 | 3 | 432 | 1.28e-19 | 91.7 |
| MsG0780036557.01.T01 | AT3G29720 | 32.407 | 216 | 111 | 10 | 109 | 313 | 10 | 201 | 5.94e-16 | 77.4 |
| MsG0780036557.01.T01 | AT5G57840 | 23.355 | 471 | 290 | 16 | 35 | 487 | 21 | 438 | 2.11e-15 | 79.0 |
| MsG0780036557.01.T01 | AT3G29690 | 29.801 | 151 | 100 | 2 | 338 | 487 | 33 | 178 | 6.47e-15 | 73.6 |
| MsG0780036557.01.T01 | AT3G50280 | 22.951 | 427 | 269 | 15 | 29 | 443 | 18 | 396 | 4.63e-14 | 74.7 |
| MsG0780036557.01.T01 | AT2G39980 | 23.505 | 485 | 333 | 16 | 11 | 476 | 1 | 466 | 4.91e-14 | 74.7 |
| MsG0780036557.01.T01 | AT5G07850 | 23.490 | 447 | 286 | 15 | 11 | 443 | 1 | 405 | 2.08e-12 | 69.7 |
| MsG0780036557.01.T01 | AT1G28680 | 20.867 | 369 | 248 | 8 | 77 | 442 | 62 | 389 | 2.90e-12 | 68.9 |
| MsG0780036557.01.T01 | AT1G31490 | 25.747 | 435 | 246 | 19 | 35 | 454 | 26 | 398 | 1.50e-11 | 66.6 |
| MsG0780036557.01.T01 | AT4G31910 | 22.714 | 339 | 226 | 7 | 70 | 399 | 57 | 368 | 1.55e-11 | 66.6 |
| MsG0780036557.01.T01 | AT3G48720 | 22.642 | 371 | 232 | 12 | 77 | 439 | 61 | 384 | 2.70e-11 | 65.9 |
| MsG0780036557.01.T01 | AT5G41040 | 23.380 | 432 | 256 | 21 | 71 | 483 | 63 | 438 | 3.31e-11 | 65.9 |
| MsG0780036557.01.T01 | AT5G41040 | 23.380 | 432 | 256 | 21 | 71 | 483 | 79 | 454 | 5.28e-11 | 65.1 |
Find 76 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGGGAGTGATTGTGAATT+TGG | 0.129194 | 7:-9760625 | None:intergenic |
| AACAGACTATCAAAATTATT+TGG | 0.185965 | 7:-9761132 | None:intergenic |
| GACATTGACGTCTAAATATT+TGG | 0.199250 | 7:+9761653 | MsG0780036557.01.T01:CDS |
| TCACTTTCTTTGACTTGTTT+TGG | 0.206968 | 7:+9760560 | MsG0780036557.01.T01:CDS |
| ATAGTCTGTTCATAAATAAT+TGG | 0.207406 | 7:+9761145 | MsG0780036557.01.T01:CDS |
| TTGAAAGGTGGTGTTGAATT+TGG | 0.210135 | 7:+9761828 | MsG0780036557.01.T01:CDS |
| GAGAGTGAAGATTTGAGTTT+TGG | 0.216722 | 7:-9760670 | None:intergenic |
| GATAATTGGTTTGCAGAATT+AGG | 0.221219 | 7:-9760529 | None:intergenic |
| CAGCTAAAGGGAGGAAGTTT+TGG | 0.225428 | 7:-9760702 | None:intergenic |
| ACAAAAGTTGACAATTTCTT+AGG | 0.235091 | 7:-9761357 | None:intergenic |
| TACTACTCCCCTCTCCAAAA+AGG | 0.248355 | 7:-9761103 | None:intergenic |
| ATTGTTGCTAATAAAATTTA+TGG | 0.257747 | 7:+9761588 | MsG0780036557.01.T01:CDS |
| GGAGTATATGAAATTGATTT+TGG | 0.266147 | 7:+9761729 | MsG0780036557.01.T01:CDS |
| TTGAATTTGATGAATGGTTT+TGG | 0.270000 | 7:-9760751 | None:intergenic |
| CGAACAGAATCCTTAACTTT+TGG | 0.279952 | 7:-9761237 | None:intergenic |
| AAGTGAGAGGGAGAGATAAT+TGG | 0.293242 | 7:-9760543 | None:intergenic |
| TCCAAATGGTACATTGTAAA+AGG | 0.320135 | 7:+9761315 | MsG0780036557.01.T01:CDS |
| TGTTTCCTTCGATAAAGTTT+TGG | 0.341922 | 7:+9760825 | MsG0780036557.01.T01:CDS |
| CTTTAGCTGGTAACATCGTT+TGG | 0.362068 | 7:+9760716 | MsG0780036557.01.T01:CDS |
| TCTAAATATTTGGCTATGAT+TGG | 0.368222 | 7:+9761663 | MsG0780036557.01.T01:CDS |
| ATTGATAGAGGATTAACTAT+TGG | 0.368461 | 7:+9761786 | MsG0780036557.01.T01:CDS |
| CTCATTTGAACCAAAAGTTA+AGG | 0.369807 | 7:+9761227 | MsG0780036557.01.T01:CDS |
| TGGACTTATGAAAATGTGAT+AGG | 0.374029 | 7:-9761176 | None:intergenic |
| AAGGGAGTGATTGTGAATTT+GGG | 0.381295 | 7:-9760624 | None:intergenic |
| TATTGGTTTAACAGAAAGTA+AGG | 0.387767 | 7:+9761803 | MsG0780036557.01.T01:CDS |
| AAGGAAACTCCGTCATCATT+TGG | 0.395044 | 7:-9760775 | None:intergenic |
| GCCATTTGGAAGATGATGTT+TGG | 0.401380 | 7:-9760460 | None:intergenic |
| ATGGATAAGGGTGTTCTTGA+TGG | 0.402404 | 7:+9761624 | MsG0780036557.01.T01:CDS |
| CCAGTTCCTGAAAACTACTT+TGG | 0.404805 | 7:+9761498 | MsG0780036557.01.T01:CDS |
| ATTTAGAGAAATTAAACAAA+AGG | 0.406967 | 7:+9761286 | MsG0780036557.01.T01:CDS |
| ATTCAAGGAATAGGAGTTGC+AGG | 0.408252 | 7:+9761693 | MsG0780036557.01.T01:CDS |
| GGTGAAGGAATTCAAGGAAT+AGG | 0.425888 | 7:+9761684 | MsG0780036557.01.T01:CDS |
| TAGTCTGTTCATAAATAATT+GGG | 0.426788 | 7:+9761146 | MsG0780036557.01.T01:CDS |
| ACAAAAGGGTGTTGTCCAAA+TGG | 0.444540 | 7:+9761301 | MsG0780036557.01.T01:CDS |
| GAGATTACTTCAATTGATAG+AGG | 0.456941 | 7:+9761774 | MsG0780036557.01.T01:CDS |
| CAAAACTTCCTCCCTTTAGC+TGG | 0.462356 | 7:+9760703 | MsG0780036557.01.T01:CDS |
| TTTAGAGAAATTAAACAAAA+GGG | 0.466166 | 7:+9761287 | MsG0780036557.01.T01:CDS |
| TTTGGATTGAATTTGATGAA+TGG | 0.473082 | 7:-9760757 | None:intergenic |
| AACAAGTCAAAGAAAGTGAG+AGG | 0.479248 | 7:-9760556 | None:intergenic |
| TTTGGAGAGGGGAGTAGTAA+AGG | 0.482127 | 7:+9761107 | MsG0780036557.01.T01:CDS |
| ACACACCATGCTGTTCTTGA+CGG | 0.482364 | 7:+9760982 | MsG0780036557.01.T01:CDS |
| GAGAAACTTTGCATTGTTCG+TGG | 0.482505 | 7:-9760507 | None:intergenic |
| CAACCATGTTCATCAAAGCT+TGG | 0.484613 | 7:+9761013 | MsG0780036557.01.T01:CDS |
| TATCGCCTTTGCTATACAAA+CGG | 0.491635 | 7:-9761400 | None:intergenic |
| GGTTGTTTGGAGACGCCATT+TGG | 0.494463 | 7:-9760474 | None:intergenic |
| CAAACGATGTTACCAGCTAA+AGG | 0.503485 | 7:-9760715 | None:intergenic |
| ATGATTGGTGAAGGAATTCA+AGG | 0.503765 | 7:+9761678 | MsG0780036557.01.T01:CDS |
| AGTTGCAGGGTCAACAAGGT+TGG | 0.506239 | 7:+9761707 | MsG0780036557.01.T01:CDS |
| TTTCGGAAGGCCTTCAAAAG+TGG | 0.513160 | 7:+9761752 | MsG0780036557.01.T01:CDS |
| AAACGATGTTACCAGCTAAA+GGG | 0.515290 | 7:-9760714 | None:intergenic |
| AATCTTTAAGCTTCTCACAT+TGG | 0.519023 | 7:-9761196 | None:intergenic |
| ACAGAAAGTAAGGATTTGAA+AGG | 0.528561 | 7:+9761813 | MsG0780036557.01.T01:CDS |
| ACCAAACATCATCTTCCAAA+TGG | 0.530125 | 7:+9760459 | MsG0780036557.01.T01:CDS |
| GTTGCAGGGTCAACAAGGTT+GGG | 0.534201 | 7:+9761708 | MsG0780036557.01.T01:CDS |
| TGGATAAGGGTGTTCTTGAT+GGG | 0.534245 | 7:+9761625 | MsG0780036557.01.T01:CDS |
| ATTACATTGTGTTAAGAGCA+AGG | 0.536608 | 7:-9760794 | None:intergenic |
| TGTTCTTGAGAAGCAAGTGA+TGG | 0.541641 | 7:+9761854 | MsG0780036557.01.T01:CDS |
| TGTTTCCGTTTGTATAGCAA+AGG | 0.550669 | 7:+9761395 | MsG0780036557.01.T01:CDS |
| GAAAGTAAGGATTTGAAAGG+TGG | 0.551682 | 7:+9761816 | MsG0780036557.01.T01:CDS |
| AATCTTGATCTGCAATCCAC+AGG | 0.565697 | 7:-9761468 | None:intergenic |
| GTGCTCCAAAACTTTATCGA+AGG | 0.566459 | 7:-9760830 | None:intergenic |
| TATTTGGCTATGATTGGTGA+AGG | 0.577286 | 7:+9761669 | MsG0780036557.01.T01:CDS |
| TAGAAGTAGATACGTTCACC+TGG | 0.583143 | 7:-9760595 | None:intergenic |
| GGAGTGATTGTGAATTTGGG+AGG | 0.595309 | 7:-9760621 | None:intergenic |
| GAATCTGTTGATTCTAAGTG+AGG | 0.596592 | 7:-9760892 | None:intergenic |
| AGTTAAGGATTCTGTTCGAG+TGG | 0.604204 | 7:+9761242 | MsG0780036557.01.T01:CDS |
| AAATTCAATCCAAATGATGA+CGG | 0.606900 | 7:+9760766 | MsG0780036557.01.T01:CDS |
| AGACCAAGCTTTGATGAACA+TGG | 0.608686 | 7:-9761016 | None:intergenic |
| ACAAGTCAAAGAAAGTGAGA+GGG | 0.619012 | 7:-9760555 | None:intergenic |
| TAGGAGTTGCAGGGTCAACA+AGG | 0.623066 | 7:+9761703 | MsG0780036557.01.T01:CDS |
| GAGTGATTGTGAATTTGGGA+GGG | 0.625337 | 7:-9760620 | None:intergenic |
| CGAGACAAAGAAGCTTCTTG+TGG | 0.646679 | 7:-9760859 | None:intergenic |
| TTCAAGGAATAGGAGTTGCA+GGG | 0.658447 | 7:+9761694 | MsG0780036557.01.T01:CDS |
| CGATGTTACCAGCTAAAGGG+AGG | 0.666686 | 7:-9760711 | None:intergenic |
| GTGGTATTCAAGCTCACACG+CGG | 0.700184 | 7:+9761261 | MsG0780036557.01.T01:CDS |
| AGAAGTAGATACGTTCACCT+GGG | 0.704297 | 7:-9760594 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGTTGCTAATAAAATTTA+TGG | + | Chr7:9761588-9761607 | MsG0780036557.01.T01:CDS | 15.0% |
| !! | ATTTAGAGAAATTAAACAAA+AGG | + | Chr7:9761286-9761305 | MsG0780036557.01.T01:CDS | 15.0% |
| !! | TTTAGAGAAATTAAACAAAA+GGG | + | Chr7:9761287-9761306 | MsG0780036557.01.T01:CDS | 15.0% |
| !! | AACAGACTATCAAAATTATT+TGG | - | Chr7:9761135-9761154 | None:intergenic | 20.0% |
| !! | ATAGTCTGTTCATAAATAAT+TGG | + | Chr7:9761145-9761164 | MsG0780036557.01.T01:CDS | 20.0% |
| !! | TAGTCTGTTCATAAATAATT+GGG | + | Chr7:9761146-9761165 | MsG0780036557.01.T01:CDS | 20.0% |
| !!! | TATGAAATTGATTTTGGTTT+CGG | + | Chr7:9761735-9761754 | MsG0780036557.01.T01:CDS | 20.0% |
| ! | AAAACAAAAACGATGGATAA+GGG | + | Chr7:9761612-9761631 | MsG0780036557.01.T01:CDS | 25.0% |
| ! | AAATTCAATCCAAATGATGA+CGG | + | Chr7:9760766-9760785 | MsG0780036557.01.T01:CDS | 25.0% |
| ! | ACAAAAGTTGACAATTTCTT+AGG | - | Chr7:9761360-9761379 | None:intergenic | 25.0% |
| ! | ATTGATAGAGGATTAACTAT+TGG | + | Chr7:9761786-9761805 | MsG0780036557.01.T01:CDS | 25.0% |
| ! | TCTAAAAAAGGATCTAAGTT+TGG | - | Chr7:9761081-9761100 | None:intergenic | 25.0% |
| ! | TCTAAATATTTGGCTATGAT+TGG | + | Chr7:9761663-9761682 | MsG0780036557.01.T01:CDS | 25.0% |
| ! | TTTGGATTGAATTTGATGAA+TGG | - | Chr7:9760760-9760779 | None:intergenic | 25.0% |
| !! | AGGTTTTCAAAAAAGAAAGA+AGG | - | Chr7:9760646-9760665 | None:intergenic | 25.0% |
| !! | GGTTTTCAAAAAAGAAAGAA+GGG | - | Chr7:9760645-9760664 | None:intergenic | 25.0% |
| !! | TTGAATTTGATGAATGGTTT+TGG | - | Chr7:9760754-9760773 | None:intergenic | 25.0% |
| !!! | AAAAACCAAGTTTTGGAAAA+AGG | - | Chr7:9760948-9760967 | None:intergenic | 25.0% |
| !!! | AAAACCAAGTTTTGGAAAAA+GGG | - | Chr7:9760947-9760966 | None:intergenic | 25.0% |
| !!! | ATTTTCTTTTAGTTTTCCTG+TGG | + | Chr7:9761452-9761471 | MsG0780036557.01.T01:CDS | 25.0% |
| !!! | GGAGTATATGAAATTGATTT+TGG | + | Chr7:9761729-9761748 | MsG0780036557.01.T01:CDS | 25.0% |
| !!! | TATTGGTTTAACAGAAAGTA+AGG | + | Chr7:9761803-9761822 | MsG0780036557.01.T01:CDS | 25.0% |
| !!! | TTTCTGTTTTTTATCGCTTT+CGG | - | Chr7:9761433-9761452 | None:intergenic | 25.0% |
| AAAAAAGGATCTAAGTTTGG+TGG | - | Chr7:9761078-9761097 | None:intergenic | 30.0% | |
| AATCTTTAAGCTTCTCACAT+TGG | - | Chr7:9761199-9761218 | None:intergenic | 30.0% | |
| ACAGAAAGTAAGGATTTGAA+AGG | + | Chr7:9761813-9761832 | MsG0780036557.01.T01:CDS | 30.0% | |
| ATTACATTGTGTTAAGAGCA+AGG | - | Chr7:9760797-9760816 | None:intergenic | 30.0% | |
| CAAAACAAAAACGATGGATA+AGG | + | Chr7:9761611-9761630 | MsG0780036557.01.T01:CDS | 30.0% | |
| CTCATTTGAACCAAAAGTTA+AGG | + | Chr7:9761227-9761246 | MsG0780036557.01.T01:CDS | 30.0% | |
| GACATTGACGTCTAAATATT+TGG | + | Chr7:9761653-9761672 | MsG0780036557.01.T01:CDS | 30.0% | |
| GAGATTACTTCAATTGATAG+AGG | + | Chr7:9761774-9761793 | MsG0780036557.01.T01:CDS | 30.0% | |
| TCCAAATGGTACATTGTAAA+AGG | + | Chr7:9761315-9761334 | MsG0780036557.01.T01:CDS | 30.0% | |
| TGGACTTATGAAAATGTGAT+AGG | - | Chr7:9761179-9761198 | None:intergenic | 30.0% | |
| TTATGGCAAAACAAAAACGA+TGG | + | Chr7:9761605-9761624 | MsG0780036557.01.T01:CDS | 30.0% | |
| ! | ATAACCCTTTTTCCAAAACT+TGG | + | Chr7:9760940-9760959 | MsG0780036557.01.T01:CDS | 30.0% |
| ! | CAATTTCCAAAGTAGTTTTC+AGG | - | Chr7:9761507-9761526 | None:intergenic | 30.0% |
| ! | CCAATAGAAAAACCAAGTTT+TGG | - | Chr7:9760955-9760974 | None:intergenic | 30.0% |
| ! | GATGATTTTACAAAGGAAGA+TGG | + | Chr7:9761558-9761577 | MsG0780036557.01.T01:CDS | 30.0% |
| ! | TCCTTTTACAATGTACCATT+TGG | - | Chr7:9761319-9761338 | None:intergenic | 30.0% |
| ! | TGAGTCAGATGATTTTACAA+AGG | + | Chr7:9761551-9761570 | MsG0780036557.01.T01:CDS | 30.0% |
| ! | TGTTTCCTTCGATAAAGTTT+TGG | + | Chr7:9760825-9760844 | MsG0780036557.01.T01:CDS | 30.0% |
| ! | TTCAAGCAATTTTTCGTGAA+GGG | + | Chr7:9761883-9761902 | MsG0780036557.01.T01:CDS | 30.0% |
| ! | TTTCAAGCAATTTTTCGTGA+AGG | + | Chr7:9761882-9761901 | MsG0780036557.01.T01:CDS | 30.0% |
| ! | TTTTACAAAGGAAGATGGTT+TGG | + | Chr7:9761563-9761582 | MsG0780036557.01.T01:CDS | 30.0% |
| !! | CCAAAACTTGGTTTTTCTAT+TGG | + | Chr7:9760952-9760971 | MsG0780036557.01.T01:CDS | 30.0% |
| !! | GATAATTGGTTTGCAGAATT+AGG | - | Chr7:9760532-9760551 | None:intergenic | 30.0% |
| !! | TCCAAAAAGGGTTCTAAAAA+AGG | - | Chr7:9761093-9761112 | None:intergenic | 30.0% |
| !!! | AAATTGATTTTGGTTTCGGA+AGG | + | Chr7:9761739-9761758 | MsG0780036557.01.T01:CDS | 30.0% |
| !!! | AAGATTTGAGTTTTGGAACA+AGG | - | Chr7:9760666-9760685 | None:intergenic | 30.0% |
| !!! | TCACTTTCTTTGACTTGTTT+TGG | + | Chr7:9760560-9760579 | MsG0780036557.01.T01:CDS | 30.0% |
| !!! | TCCTTTTTTAGAACCCTTTT+TGG | + | Chr7:9761089-9761108 | MsG0780036557.01.T01:CDS | 30.0% |
| AAACGATGTTACCAGCTAAA+GGG | - | Chr7:9760717-9760736 | None:intergenic | 35.0% | |
| AACAAGTCAAAGAAAGTGAG+AGG | - | Chr7:9760559-9760578 | None:intergenic | 35.0% | |
| AAGGGAGTGATTGTGAATTT+GGG | - | Chr7:9760627-9760646 | None:intergenic | 35.0% | |
| ACAAGTCAAAGAAAGTGAGA+GGG | - | Chr7:9760558-9760577 | None:intergenic | 35.0% | |
| ACCAAACATCATCTTCCAAA+TGG | + | Chr7:9760459-9760478 | MsG0780036557.01.T01:CDS | 35.0% | |
| ATGATTGGTGAAGGAATTCA+AGG | + | Chr7:9761678-9761697 | MsG0780036557.01.T01:CDS | 35.0% | |
| GAAAGTAAGGATTTGAAAGG+TGG | + | Chr7:9761816-9761835 | MsG0780036557.01.T01:CDS | 35.0% | |
| TATTTGGCTATGATTGGTGA+AGG | + | Chr7:9761669-9761688 | MsG0780036557.01.T01:CDS | 35.0% | |
| TGTTTCCGTTTGTATAGCAA+AGG | + | Chr7:9761395-9761414 | MsG0780036557.01.T01:CDS | 35.0% | |
| ! | CGAACAGAATCCTTAACTTT+TGG | - | Chr7:9761240-9761259 | None:intergenic | 35.0% |
| ! | TATCGCCTTTGCTATACAAA+CGG | - | Chr7:9761403-9761422 | None:intergenic | 35.0% |
| ! | TTGAAAGGTGGTGTTGAATT+TGG | + | Chr7:9761828-9761847 | MsG0780036557.01.T01:CDS | 35.0% |
| !! | ATTGTTCGTGGATTTTGATG+TGG | - | Chr7:9760498-9760517 | None:intergenic | 35.0% |
| !! | GAAGTAATCTCCACTTTTGA+AGG | - | Chr7:9761765-9761784 | None:intergenic | 35.0% |
| !! | GAATCTGTTGATTCTAAGTG+AGG | - | Chr7:9760895-9760914 | None:intergenic | 35.0% |
| !! | GAGAGTGAAGATTTGAGTTT+TGG | - | Chr7:9760673-9760692 | None:intergenic | 35.0% |
| !! | TGGATTTTGATGTGGTTGTT+TGG | - | Chr7:9760490-9760509 | None:intergenic | 35.0% |
| !! | TTTAGAACCCTTTTTGGAGA+GGG | + | Chr7:9761095-9761114 | MsG0780036557.01.T01:CDS | 35.0% |
| !!! | AATGGTTTTGGAGAATCAGA+AGG | - | Chr7:9760742-9760761 | None:intergenic | 35.0% |
| !!! | TCTTTGACTTGTTTTGGCTA+AGG | + | Chr7:9760566-9760585 | MsG0780036557.01.T01:CDS | 35.0% |
| !!! | TTTTAGAACCCTTTTTGGAG+AGG | + | Chr7:9761094-9761113 | MsG0780036557.01.T01:CDS | 35.0% |
| AAGGAAACTCCGTCATCATT+TGG | - | Chr7:9760778-9760797 | None:intergenic | 40.0% | |
| AAGTGAGAGGGAGAGATAAT+TGG | - | Chr7:9760546-9760565 | None:intergenic | 40.0% | |
| AATCTTGATCTGCAATCCAC+AGG | - | Chr7:9761471-9761490 | None:intergenic | 40.0% | |
| AGAAGTAGATACGTTCACCT+GGG | - | Chr7:9760597-9760616 | None:intergenic | 40.0% | |
| AGTTAAGGATTCTGTTCGAG+TGG | + | Chr7:9761242-9761261 | MsG0780036557.01.T01:CDS | 40.0% | |
| ATTCAAGGAATAGGAGTTGC+AGG | + | Chr7:9761693-9761712 | MsG0780036557.01.T01:CDS | 40.0% | |
| CAAACGATGTTACCAGCTAA+AGG | - | Chr7:9760718-9760737 | None:intergenic | 40.0% | |
| CAACCATGTTCATCAAAGCT+TGG | + | Chr7:9761013-9761032 | MsG0780036557.01.T01:CDS | 40.0% | |
| CCAGTTCCTGAAAACTACTT+TGG | + | Chr7:9761498-9761517 | MsG0780036557.01.T01:CDS | 40.0% | |
| CTTTAGCTGGTAACATCGTT+TGG | + | Chr7:9760716-9760735 | MsG0780036557.01.T01:CDS | 40.0% | |
| GAAGGGAGTGATTGTGAATT+TGG | - | Chr7:9760628-9760647 | None:intergenic | 40.0% | |
| GAGAAACTTTGCATTGTTCG+TGG | - | Chr7:9760510-9760529 | None:intergenic | 40.0% | |
| GAGTGATTGTGAATTTGGGA+GGG | - | Chr7:9760623-9760642 | None:intergenic | 40.0% | |
| GCCATTTGGAAGATGATGTT+TGG | - | Chr7:9760463-9760482 | None:intergenic | 40.0% | |
| GGTGAAGGAATTCAAGGAAT+AGG | + | Chr7:9761684-9761703 | MsG0780036557.01.T01:CDS | 40.0% | |
| GTGCTCCAAAACTTTATCGA+AGG | - | Chr7:9760833-9760852 | None:intergenic | 40.0% | |
| TAGAAGTAGATACGTTCACC+TGG | - | Chr7:9760598-9760617 | None:intergenic | 40.0% | |
| TGTTCTTGAGAAGCAAGTGA+TGG | + | Chr7:9761854-9761873 | MsG0780036557.01.T01:CDS | 40.0% | |
| TTCAAGGAATAGGAGTTGCA+GGG | + | Chr7:9761694-9761713 | MsG0780036557.01.T01:CDS | 40.0% | |
| ! | AGACCAAGCTTTGATGAACA+TGG | - | Chr7:9761019-9761038 | None:intergenic | 40.0% |
| ! | ATTTTCCGTCAAGAACAGCA+TGG | - | Chr7:9760990-9761009 | None:intergenic | 40.0% |
| !! | AAGTAGTTTTCAGGAACTGG+TGG | - | Chr7:9761498-9761517 | None:intergenic | 40.0% |
| !! | ACAAAAGGGTGTTGTCCAAA+TGG | + | Chr7:9761301-9761320 | MsG0780036557.01.T01:CDS | 40.0% |
| !! | ATGGATAAGGGTGTTCTTGA+TGG | + | Chr7:9761624-9761643 | MsG0780036557.01.T01:CDS | 40.0% |
| !! | CCAAAGTAGTTTTCAGGAAC+TGG | - | Chr7:9761501-9761520 | None:intergenic | 40.0% |
| !! | TGGATAAGGGTGTTCTTGAT+GGG | + | Chr7:9761625-9761644 | MsG0780036557.01.T01:CDS | 40.0% |
| !! | TTAGAACCCTTTTTGGAGAG+GGG | + | Chr7:9761096-9761115 | MsG0780036557.01.T01:CDS | 40.0% |
| !!! | TAAAGGGAGGAAGTTTTGGA+GGG | - | Chr7:9760701-9760720 | None:intergenic | 40.0% |
| ACACACCATGCTGTTCTTGA+CGG | + | Chr7:9760982-9761001 | MsG0780036557.01.T01:CDS | 45.0% | |
| ACTACTCCCCTCTCCAAAAA+GGG | - | Chr7:9761105-9761124 | None:intergenic | 45.0% | |
| CAAAACTTCCTCCCTTTAGC+TGG | + | Chr7:9760703-9760722 | MsG0780036557.01.T01:CDS | 45.0% | |
| CGAGACAAAGAAGCTTCTTG+TGG | - | Chr7:9760862-9760881 | None:intergenic | 45.0% | |
| GGAGTGATTGTGAATTTGGG+AGG | - | Chr7:9760624-9760643 | None:intergenic | 45.0% | |
| TACTACTCCCCTCTCCAAAA+AGG | - | Chr7:9761106-9761125 | None:intergenic | 45.0% | |
| TTTCGGAAGGCCTTCAAAAG+TGG | + | Chr7:9761752-9761771 | MsG0780036557.01.T01:CDS | 45.0% | |
| TTTGGAGAGGGGAGTAGTAA+AGG | + | Chr7:9761107-9761126 | MsG0780036557.01.T01:CDS | 45.0% | |
| ! | CAGCTAAAGGGAGGAAGTTT+TGG | - | Chr7:9760705-9760724 | None:intergenic | 45.0% |
| !! | CTAAAGGGAGGAAGTTTTGG+AGG | - | Chr7:9760702-9760721 | None:intergenic | 45.0% |
| !! | TTTTGGCTAAGGTTTCACCC+AGG | + | Chr7:9760577-9760596 | MsG0780036557.01.T01:CDS | 45.0% |
| AGTTGCAGGGTCAACAAGGT+TGG | + | Chr7:9761707-9761726 | MsG0780036557.01.T01:CDS | 50.0% | |
| CGATGTTACCAGCTAAAGGG+AGG | - | Chr7:9760714-9760733 | None:intergenic | 50.0% | |
| GGTTGTTTGGAGACGCCATT+TGG | - | Chr7:9760477-9760496 | None:intergenic | 50.0% | |
| GTGGTATTCAAGCTCACACG+CGG | + | Chr7:9761261-9761280 | MsG0780036557.01.T01:CDS | 50.0% | |
| GTTGCAGGGTCAACAAGGTT+GGG | + | Chr7:9761708-9761727 | MsG0780036557.01.T01:CDS | 50.0% | |
| TAGGAGTTGCAGGGTCAACA+AGG | + | Chr7:9761703-9761722 | MsG0780036557.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 9760448 | 9761920 | 9760448 | ID=MsG0780036557.01;Name=MsG0780036557.01 |
| Chr7 | mRNA | 9760448 | 9761920 | 9760448 | ID=MsG0780036557.01.T01;Parent=MsG0780036557.01;Name=MsG0780036557.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|490 |
| Chr7 | exon | 9760448 | 9761920 | 9760448 | ID=MsG0780036557.01.T01:exon:1510;Parent=MsG0780036557.01.T01 |
| Chr7 | CDS | 9760448 | 9761920 | 9760448 | ID=MsG0780036557.01.T01:cds;Parent=MsG0780036557.01.T01 |
| Gene Sequence |
| Protein sequence |
