Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036556.01.T01 | XP_013447578.1 | 81.237 | 485 | 50 | 7 | 1 | 446 | 1 | 483 | 0 | 762 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036556.01.T01 | Q9LJB4 | 36.188 | 467 | 254 | 10 | 1 | 442 | 1 | 448 | 1.72E-77 | 251 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036556.01.T01 | B4Y0U1 | 81.237 | 485 | 50 | 7 | 1 | 446 | 1 | 483 | 0.0 | 762 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580025771.01 | MsG0780036556.01 | 0.805048 | 1.610746e-49 | 8.436808e-47 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036556.01.T01 | MTR_7g013850 | 81.237 | 485 | 50 | 7 | 1 | 446 | 1 | 483 | 0.0 | 762 |
| MsG0780036556.01.T01 | MTR_7g014200 | 57.415 | 472 | 158 | 11 | 5 | 442 | 6 | 468 | 1.89e-177 | 505 |
| MsG0780036556.01.T01 | MTR_7g014160 | 55.042 | 476 | 168 | 11 | 5 | 442 | 6 | 473 | 1.34e-170 | 488 |
| MsG0780036556.01.T01 | MTR_7g014610 | 54.658 | 483 | 170 | 11 | 1 | 442 | 1 | 475 | 7.51e-161 | 463 |
| MsG0780036556.01.T01 | MTR_7g014620 | 55.402 | 435 | 174 | 10 | 20 | 442 | 6 | 432 | 1.93e-149 | 432 |
| MsG0780036556.01.T01 | MTR_6g015315 | 37.908 | 459 | 248 | 9 | 9 | 442 | 6 | 452 | 1.09e-93 | 290 |
| MsG0780036556.01.T01 | MTR_6g015830 | 39.744 | 468 | 232 | 14 | 9 | 442 | 9 | 460 | 3.62e-92 | 286 |
| MsG0780036556.01.T01 | MTR_6g015320 | 37.821 | 468 | 251 | 11 | 1 | 442 | 1 | 454 | 4.30e-92 | 286 |
| MsG0780036556.01.T01 | MTR_6g015850 | 39.789 | 475 | 235 | 16 | 1 | 442 | 1 | 457 | 5.16e-92 | 286 |
| MsG0780036556.01.T01 | MTR_7g014630 | 67.453 | 212 | 63 | 4 | 1 | 210 | 1 | 208 | 1.32e-91 | 282 |
| MsG0780036556.01.T01 | MTR_6g015815 | 40.088 | 454 | 222 | 14 | 20 | 442 | 26 | 460 | 3.70e-89 | 279 |
| MsG0780036556.01.T01 | MTR_7g014330 | 36.632 | 475 | 239 | 14 | 9 | 442 | 3 | 456 | 7.22e-84 | 265 |
| MsG0780036556.01.T01 | MTR_7g014230 | 36.752 | 468 | 246 | 13 | 9 | 441 | 5 | 457 | 1.20e-83 | 265 |
| MsG0780036556.01.T01 | MTR_7g014250 | 35.579 | 475 | 244 | 13 | 9 | 442 | 3 | 456 | 2.32e-82 | 261 |
| MsG0780036556.01.T01 | MTR_7g014180 | 68.715 | 179 | 49 | 5 | 1 | 178 | 1 | 173 | 9.50e-74 | 229 |
| MsG0780036556.01.T01 | MTR_7g014670 | 58.421 | 190 | 77 | 2 | 254 | 442 | 71 | 259 | 3.05e-70 | 223 |
| MsG0780036556.01.T01 | MTR_3g028210 | 34.388 | 474 | 269 | 13 | 1 | 442 | 1 | 464 | 1.44e-68 | 226 |
| MsG0780036556.01.T01 | MTR_2g089765 | 30.932 | 472 | 276 | 13 | 11 | 442 | 21 | 482 | 1.88e-60 | 205 |
| MsG0780036556.01.T01 | MTR_7g014360 | 32.115 | 383 | 227 | 10 | 76 | 442 | 21 | 386 | 1.59e-55 | 189 |
| MsG0780036556.01.T01 | MTR_2g089670 | 31.696 | 448 | 258 | 14 | 28 | 442 | 45 | 477 | 6.53e-55 | 190 |
| MsG0780036556.01.T01 | MTR_2g089650 | 29.730 | 481 | 281 | 15 | 1 | 442 | 1 | 463 | 1.80e-50 | 178 |
| MsG0780036556.01.T01 | MTR_2g089755 | 30.330 | 455 | 261 | 13 | 28 | 442 | 37 | 475 | 1.72e-49 | 175 |
| MsG0780036556.01.T01 | MTR_7g015860 | 30.020 | 493 | 258 | 19 | 4 | 442 | 1 | 460 | 4.98e-49 | 174 |
| MsG0780036556.01.T01 | MTR_3g087640 | 30.408 | 490 | 264 | 14 | 9 | 445 | 10 | 475 | 9.70e-49 | 173 |
| MsG0780036556.01.T01 | MTR_2g089735 | 30.752 | 439 | 251 | 13 | 41 | 442 | 39 | 461 | 1.92e-48 | 172 |
| MsG0780036556.01.T01 | MTR_8g012750 | 29.583 | 480 | 279 | 19 | 8 | 440 | 3 | 470 | 5.45e-46 | 166 |
| MsG0780036556.01.T01 | MTR_6g015310 | 30.263 | 380 | 185 | 11 | 63 | 420 | 12 | 333 | 1.69e-40 | 147 |
| MsG0780036556.01.T01 | MTR_7g014310 | 30.100 | 299 | 173 | 7 | 130 | 425 | 41 | 306 | 1.21e-34 | 131 |
| MsG0780036556.01.T01 | MTR_2g089665 | 28.571 | 420 | 192 | 17 | 11 | 415 | 7 | 333 | 6.05e-30 | 119 |
| MsG0780036556.01.T01 | MTR_3g435300 | 34.555 | 191 | 105 | 6 | 9 | 187 | 4 | 186 | 1.84e-21 | 94.0 |
| MsG0780036556.01.T01 | MTR_7g086000 | 23.462 | 439 | 252 | 14 | 7 | 394 | 3 | 408 | 1.47e-20 | 94.4 |
| MsG0780036556.01.T01 | MTR_3g070170 | 24.074 | 432 | 266 | 15 | 8 | 398 | 12 | 422 | 8.40e-19 | 89.0 |
| MsG0780036556.01.T01 | MTR_3g088420 | 23.831 | 449 | 267 | 16 | 17 | 432 | 14 | 420 | 1.22e-17 | 85.1 |
| MsG0780036556.01.T01 | MTR_4g094838 | 25.688 | 436 | 267 | 15 | 19 | 432 | 19 | 419 | 1.72e-17 | 84.7 |
| MsG0780036556.01.T01 | MTR_3g101970 | 25.280 | 447 | 292 | 18 | 8 | 436 | 6 | 428 | 2.21e-17 | 84.3 |
| MsG0780036556.01.T01 | MTR_6g045553 | 22.981 | 483 | 286 | 16 | 3 | 431 | 11 | 461 | 2.30e-17 | 84.7 |
| MsG0780036556.01.T01 | MTR_4g022290 | 23.077 | 442 | 281 | 13 | 19 | 432 | 22 | 432 | 3.74e-17 | 83.6 |
| MsG0780036556.01.T01 | MTR_3g101960 | 25.000 | 400 | 245 | 15 | 28 | 398 | 29 | 402 | 1.15e-16 | 82.4 |
| MsG0780036556.01.T01 | MTR_8g075610 | 24.064 | 374 | 232 | 16 | 44 | 394 | 30 | 374 | 1.28e-16 | 82.0 |
| MsG0780036556.01.T01 | MTR_8g096530 | 24.524 | 473 | 277 | 18 | 7 | 446 | 6 | 431 | 2.63e-16 | 81.3 |
| MsG0780036556.01.T01 | MTR_4g013050 | 24.069 | 403 | 236 | 16 | 66 | 442 | 52 | 410 | 3.03e-16 | 80.9 |
| MsG0780036556.01.T01 | MTR_3g101900 | 23.364 | 428 | 289 | 12 | 28 | 435 | 30 | 438 | 3.32e-16 | 80.9 |
| MsG0780036556.01.T01 | MTR_4g094835 | 22.545 | 448 | 258 | 15 | 28 | 432 | 32 | 433 | 6.55e-16 | 80.1 |
| MsG0780036556.01.T01 | MTR_3g101930 | 25.556 | 450 | 284 | 20 | 13 | 431 | 12 | 441 | 1.18e-15 | 79.3 |
| MsG0780036556.01.T01 | MTR_8g467580 | 24.623 | 398 | 253 | 14 | 17 | 394 | 15 | 385 | 1.99e-15 | 78.6 |
| MsG0780036556.01.T01 | MTR_7g014205 | 32.258 | 124 | 72 | 2 | 282 | 404 | 2 | 114 | 2.30e-15 | 72.8 |
| MsG0780036556.01.T01 | MTR_7g014210 | 35.514 | 107 | 67 | 2 | 300 | 404 | 24 | 130 | 3.22e-15 | 73.6 |
| MsG0780036556.01.T01 | MTR_4g094828 | 22.148 | 447 | 286 | 16 | 19 | 432 | 20 | 437 | 5.19e-15 | 77.4 |
| MsG0780036556.01.T01 | MTR_5g081960 | 22.594 | 478 | 295 | 16 | 13 | 431 | 11 | 472 | 6.45e-15 | 77.0 |
| MsG0780036556.01.T01 | MTR_3g102020 | 24.943 | 437 | 271 | 17 | 31 | 436 | 32 | 442 | 2.71e-14 | 75.1 |
| MsG0780036556.01.T01 | MTR_4g094842 | 22.794 | 408 | 249 | 14 | 62 | 432 | 57 | 435 | 4.53e-14 | 74.3 |
| MsG0780036556.01.T01 | MTR_0190s0020 | 22.532 | 395 | 268 | 11 | 21 | 398 | 22 | 395 | 1.27e-13 | 72.8 |
| MsG0780036556.01.T01 | MTR_3g049150 | 24.059 | 478 | 288 | 21 | 8 | 442 | 2 | 447 | 1.85e-13 | 72.4 |
| MsG0780036556.01.T01 | MTR_3g098980 | 23.188 | 345 | 226 | 13 | 67 | 394 | 66 | 388 | 2.58e-13 | 72.0 |
| MsG0780036556.01.T01 | MTR_8g079950 | 25.333 | 450 | 275 | 17 | 11 | 435 | 8 | 421 | 3.85e-13 | 71.6 |
| MsG0780036556.01.T01 | MTR_7g014340 | 54.930 | 71 | 25 | 2 | 9 | 75 | 3 | 70 | 5.92e-13 | 65.1 |
| MsG0780036556.01.T01 | MTR_3g026310 | 22.196 | 428 | 265 | 13 | 17 | 406 | 14 | 411 | 8.72e-13 | 70.5 |
| MsG0780036556.01.T01 | MTR_2g007880 | 25.522 | 431 | 238 | 20 | 50 | 435 | 48 | 440 | 9.84e-13 | 70.1 |
| MsG0780036556.01.T01 | MTR_4g022720 | 23.543 | 446 | 274 | 17 | 17 | 432 | 14 | 422 | 1.20e-12 | 69.7 |
| MsG0780036556.01.T01 | MTR_7g026860 | 24.169 | 451 | 271 | 17 | 16 | 432 | 12 | 425 | 2.45e-12 | 68.9 |
| MsG0780036556.01.T01 | MTR_4g022825 | 23.733 | 434 | 272 | 18 | 28 | 432 | 24 | 427 | 5.57e-12 | 67.8 |
| MsG0780036556.01.T01 | MTR_7g014220 | 57.143 | 56 | 21 | 1 | 22 | 77 | 12 | 64 | 1.55e-11 | 60.5 |
| MsG0780036556.01.T01 | MTR_8g041770 | 22.489 | 458 | 290 | 15 | 7 | 442 | 13 | 427 | 2.43e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036556.01.T01 | AT3G29590 | 36.188 | 467 | 254 | 10 | 1 | 442 | 1 | 448 | 1.76e-78 | 251 |
| MsG0780036556.01.T01 | AT3G29670 | 36.383 | 459 | 256 | 10 | 8 | 442 | 3 | 449 | 3.24e-77 | 248 |
| MsG0780036556.01.T01 | AT5G39090 | 36.285 | 463 | 255 | 12 | 4 | 442 | 1 | 447 | 1.19e-75 | 243 |
| MsG0780036556.01.T01 | AT3G29635 | 35.837 | 466 | 254 | 13 | 8 | 442 | 3 | 454 | 8.97e-74 | 239 |
| MsG0780036556.01.T01 | AT5G39080 | 34.874 | 476 | 244 | 13 | 8 | 439 | 5 | 458 | 6.40e-72 | 234 |
| MsG0780036556.01.T01 | AT5G39050 | 35.787 | 489 | 237 | 15 | 4 | 442 | 6 | 467 | 1.16e-71 | 234 |
| MsG0780036556.01.T01 | AT1G03940 | 30.625 | 480 | 269 | 13 | 9 | 445 | 9 | 467 | 4.94e-65 | 216 |
| MsG0780036556.01.T01 | AT3G29680 | 33.913 | 460 | 253 | 13 | 8 | 438 | 6 | 443 | 2.91e-63 | 211 |
| MsG0780036556.01.T01 | AT5G61160 | 30.769 | 455 | 284 | 11 | 8 | 440 | 3 | 448 | 3.61e-61 | 206 |
| MsG0780036556.01.T01 | AT1G03495 | 31.461 | 356 | 198 | 12 | 1 | 324 | 1 | 342 | 9.60e-41 | 149 |
| MsG0780036556.01.T01 | AT5G23940 | 25.541 | 462 | 282 | 20 | 8 | 432 | 3 | 439 | 1.88e-23 | 103 |
| MsG0780036556.01.T01 | AT5G67150 | 25.115 | 434 | 289 | 12 | 21 | 436 | 22 | 437 | 3.45e-20 | 93.2 |
| MsG0780036556.01.T01 | AT2G19070 | 22.727 | 440 | 273 | 14 | 41 | 440 | 37 | 449 | 2.32e-19 | 90.5 |
| MsG0780036556.01.T01 | AT1G24430 | 26.302 | 384 | 240 | 14 | 67 | 436 | 62 | 416 | 7.58e-19 | 89.0 |
| MsG0780036556.01.T01 | AT3G50270 | 26.077 | 441 | 287 | 14 | 16 | 433 | 17 | 441 | 5.99e-18 | 86.3 |
| MsG0780036556.01.T01 | AT3G26040 | 26.406 | 409 | 264 | 16 | 38 | 435 | 37 | 419 | 4.05e-16 | 80.5 |
| MsG0780036556.01.T01 | AT3G29720 | 44.554 | 101 | 46 | 3 | 99 | 191 | 10 | 108 | 1.76e-15 | 75.9 |
| MsG0780036556.01.T01 | AT2G39980 | 22.153 | 483 | 307 | 13 | 1 | 431 | 1 | 466 | 2.01e-15 | 78.6 |
| MsG0780036556.01.T01 | AT3G29690 | 30.719 | 153 | 96 | 3 | 293 | 442 | 33 | 178 | 4.45e-15 | 73.6 |
| MsG0780036556.01.T01 | AT5G41040 | 23.427 | 461 | 292 | 19 | 5 | 438 | 28 | 454 | 1.75e-14 | 75.9 |
| MsG0780036556.01.T01 | AT5G41040 | 23.427 | 461 | 292 | 19 | 5 | 438 | 12 | 438 | 1.86e-14 | 75.5 |
| MsG0780036556.01.T01 | AT5G07850 | 24.057 | 424 | 277 | 15 | 1 | 398 | 1 | 405 | 2.05e-14 | 75.5 |
| MsG0780036556.01.T01 | AT5G38130 | 22.506 | 391 | 244 | 13 | 31 | 394 | 52 | 410 | 1.25e-13 | 73.2 |
| MsG0780036556.01.T01 | AT1G31490 | 27.139 | 409 | 238 | 18 | 25 | 409 | 26 | 398 | 1.88e-13 | 72.4 |
Find 63 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAGTTGGGTTGAAGAATT+AGG | 0.162139 | 7:-9747785 | None:intergenic |
| TCTAATAAAGGCTCTAAATT+TGG | 0.169522 | 7:-9748331 | None:intergenic |
| AGCCTTTATTAGAACCATTT+TGG | 0.176979 | 7:+9748341 | MsG0780036556.01.T01:CDS |
| TGTTTCCTTCGATCAAATTT+TGG | 0.183599 | 7:+9748081 | MsG0780036556.01.T01:CDS |
| TTGAAAGGTAGTATTGAATT+TGG | 0.208629 | 7:+9749086 | MsG0780036556.01.T01:CDS |
| TTTGGATTGAATTGGATTAA+TGG | 0.211797 | 7:-9748013 | None:intergenic |
| TTGAATTGGATTAATGGTTT+TGG | 0.231343 | 7:-9748007 | None:intergenic |
| TTCAATATTGTTGTTCCATT+TGG | 0.246981 | 7:-9748568 | None:intergenic |
| GAGAGCGAAGATTTGAGTTT+TGG | 0.251282 | 7:-9747926 | None:intergenic |
| ACAAAAGTGGACAGCTTCTT+AGG | 0.251396 | 7:-9748615 | None:intergenic |
| CGTCATCATTTGGATTGAAT+TGG | 0.258288 | 7:-9748021 | None:intergenic |
| GATGACTTCCCTTTCCAAAA+TGG | 0.259451 | 7:-9748355 | None:intergenic |
| TTCCAAAATGGTTCTAATAA+AGG | 0.267048 | 7:-9748343 | None:intergenic |
| GGAGTATATGAGATTGATTT+TGG | 0.276059 | 7:+9748987 | MsG0780036556.01.T01:CDS |
| ATGATTGGTGAAGGAGTTAA+AGG | 0.329186 | 7:+9748936 | MsG0780036556.01.T01:CDS |
| AACGAATTCTCAAAATCATT+TGG | 0.329734 | 7:-9748384 | None:intergenic |
| GAGAAACTTTGCATTGTTCA+TGG | 0.331192 | 7:-9747763 | None:intergenic |
| CTTTAGCTGGTAACATCGTT+TGG | 0.362068 | 7:+9747972 | MsG0780036556.01.T01:CDS |
| GTTAAAGGAATAGGAGTTGC+AGG | 0.381644 | 7:+9748951 | MsG0780036556.01.T01:CDS |
| AATTGGTTTGACAGAAAGTA+AGG | 0.387323 | 7:+9749061 | MsG0780036556.01.T01:CDS |
| AAGGAAACTCCGTCATCATT+TGG | 0.395044 | 7:-9748031 | None:intergenic |
| GGTGAAGGAGTTAAAGGAAT+AGG | 0.395384 | 7:+9748942 | MsG0780036556.01.T01:CDS |
| TGTGAGAAGGAGAGATAGTT+GGG | 0.418473 | 7:-9747798 | None:intergenic |
| ACACACCATGCTGTTCTTGA+TGG | 0.421878 | 7:+9748238 | MsG0780036556.01.T01:CDS |
| ATTGATAGAGGATTAACAAT+TGG | 0.431776 | 7:+9749044 | MsG0780036556.01.T01:CDS |
| TAGAAGAAAATACGTTCTAC+TGG | 0.445325 | 7:-9747851 | None:intergenic |
| GTTGCAGGGTCAACAAGGTT+TGG | 0.454958 | 7:+9748966 | MsG0780036556.01.T01:CDS |
| AGAATTCGTTCATTAATACT+TGG | 0.456841 | 7:+9748397 | MsG0780036556.01.T01:CDS |
| GAGATTACTTCAATTGATAG+AGG | 0.456941 | 7:+9749032 | MsG0780036556.01.T01:CDS |
| CAACACTTCCTCCCTTTAGC+TGG | 0.464034 | 7:+9747959 | MsG0780036556.01.T01:CDS |
| CAGCTAAAGGGAGGAAGTGT+TGG | 0.471757 | 7:-9747958 | None:intergenic |
| ATGTGAGAAGGAGAGATAGT+TGG | 0.473667 | 7:-9747799 | None:intergenic |
| CAAAACAAAAGAGATGGATA+AGG | 0.478383 | 7:+9748869 | MsG0780036556.01.T01:CDS |
| TATAGATATATAGCTATGAT+TGG | 0.488633 | 7:+9748921 | MsG0780036556.01.T01:CDS |
| AGCACATGTAAGAACAAAAG+TGG | 0.494080 | 7:-9748628 | None:intergenic |
| ATAACAGGGTGTTATCCAAA+TGG | 0.497827 | 7:+9748553 | MsG0780036556.01.T01:intron |
| CAAACGATGTTACCAGCTAA+AGG | 0.503485 | 7:-9747971 | None:intergenic |
| TATGACTTTACAAAAGAAGA+TGG | 0.504011 | 7:+9748816 | MsG0780036556.01.T01:CDS |
| AAAACAAAAGAGATGGATAA+GGG | 0.505500 | 7:+9748870 | MsG0780036556.01.T01:CDS |
| GATCGCCTTTGCTATACAAA+CGG | 0.514173 | 7:-9748658 | None:intergenic |
| AAACGATGTTACCAGCTAAA+GGG | 0.515290 | 7:-9747970 | None:intergenic |
| TGGAACAACAATATTGAAGA+AGG | 0.519341 | 7:+9748573 | MsG0780036556.01.T01:CDS |
| ACAGAAAGTAAGGATTTGAA+AGG | 0.525092 | 7:+9749071 | MsG0780036556.01.T01:CDS |
| GTGCTCCAAAATTTGATCGA+AGG | 0.536336 | 7:-9748086 | None:intergenic |
| GTAGGGTGTGAATGTGAACT+TGG | 0.545769 | 7:-9747881 | None:intergenic |
| TGTTCTTGATAAGCATGTGA+TGG | 0.547164 | 7:+9749112 | MsG0780036556.01.T01:CDS |
| TGTTTCCGTTTGTATAGCAA+AGG | 0.550669 | 7:+9748653 | MsG0780036556.01.T01:CDS |
| AATCTTGATCTGCAATCCAC+AGG | 0.563131 | 7:-9748726 | None:intergenic |
| TATATAGCTATGATTGGTGA+AGG | 0.570710 | 7:+9748927 | MsG0780036556.01.T01:CDS |
| TTATGTCAAAACAAAAGAGA+TGG | 0.571134 | 7:+9748863 | MsG0780036556.01.T01:CDS |
| GTGTGAATGTGAACTTGGGA+GGG | 0.578151 | 7:-9747876 | None:intergenic |
| GAATCTGATGATTCTAAGTG+AGG | 0.596592 | 7:-9748148 | None:intergenic |
| CAATTCAATCCAAATGATGA+CGG | 0.601071 | 7:+9748022 | MsG0780036556.01.T01:CDS |
| GGTGTGAATGTGAACTTGGG+AGG | 0.617310 | 7:-9747877 | None:intergenic |
| GTCACATTGTGCTAAGAGCA+AGG | 0.618079 | 7:-9748050 | None:intergenic |
| TAGGAGTTGCAGGGTCAACA+AGG | 0.619039 | 7:+9748961 | MsG0780036556.01.T01:CDS |
| CAACCATGTTCATCAAAGCA+TGG | 0.631200 | 7:+9748269 | MsG0780036556.01.T01:CDS |
| ATGGTGTGTGCTTATTCCAA+TGG | 0.644576 | 7:-9748224 | None:intergenic |
| CGAGACAAAGAAGCTTCTTG+TGG | 0.646679 | 7:-9748115 | None:intergenic |
| TAGGGTGTGAATGTGAACTT+GGG | 0.653813 | 7:-9747880 | None:intergenic |
| AGACCATGCTTTGATGAACA+TGG | 0.653845 | 7:-9748272 | None:intergenic |
| CGATGTTACCAGCTAAAGGG+AGG | 0.666686 | 7:-9747967 | None:intergenic |
| TTAAAGGAATAGGAGTTGCA+GGG | 0.673383 | 7:+9748952 | MsG0780036556.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGATGAAAATAAATAAC+AGG | + | Chr7:9748538-9748557 | MsG0780036556.01.T01:intron | 15.0% |
| !! | TTTGATGAAAATAAATAACA+GGG | + | Chr7:9748539-9748558 | MsG0780036556.01.T01:intron | 15.0% |
| !!! | CAATTTCCAAAATAATTTTT+AGG | - | Chr7:9748765-9748784 | None:intergenic | 15.0% |
| !! | TATAGATATATAGCTATGAT+TGG | + | Chr7:9748921-9748940 | MsG0780036556.01.T01:CDS | 20.0% |
| !!! | AAAAAGCAGAAATTTTCTTT+TGG | + | Chr7:9748699-9748718 | MsG0780036556.01.T01:CDS | 20.0% |
| !!! | AAATAATTTTTAGGAATTGG+TGG | - | Chr7:9748756-9748775 | None:intergenic | 20.0% |
| !!! | AGATTTTCAAAGAAAAAAGT+AGG | - | Chr7:9747902-9747921 | None:intergenic | 20.0% |
| !!! | CCAAAATAATTTTTAGGAAT+TGG | - | Chr7:9748759-9748778 | None:intergenic | 20.0% |
| !!! | CCAATTCCTAAAAATTATTT+TGG | + | Chr7:9748756-9748775 | MsG0780036556.01.T01:CDS | 20.0% |
| !!! | GATTTTCAAAGAAAAAAGTA+GGG | - | Chr7:9747901-9747920 | None:intergenic | 20.0% |
| ! | AAAACAAAAGAGATGGATAA+GGG | + | Chr7:9748870-9748889 | MsG0780036556.01.T01:CDS | 25.0% |
| ! | AACGAATTCTCAAAATCATT+TGG | - | Chr7:9748387-9748406 | None:intergenic | 25.0% |
| ! | AGAATTCGTTCATTAATACT+TGG | + | Chr7:9748397-9748416 | MsG0780036556.01.T01:CDS | 25.0% |
| ! | ATTGATAGAGGATTAACAAT+TGG | + | Chr7:9749044-9749063 | MsG0780036556.01.T01:CDS | 25.0% |
| ! | TAATAACCCTTCATGAAAAA+TGG | - | Chr7:9749150-9749169 | None:intergenic | 25.0% |
| ! | TATGACTTTACAAAAGAAGA+TGG | + | Chr7:9748816-9748835 | MsG0780036556.01.T01:CDS | 25.0% |
| ! | TTATGTCAAAACAAAAGAGA+TGG | + | Chr7:9748863-9748882 | MsG0780036556.01.T01:CDS | 25.0% |
| ! | TTTGGATTGAATTGGATTAA+TGG | - | Chr7:9748016-9748035 | None:intergenic | 25.0% |
| !! | TCTTCTTTTGTAAAGTCATA+TGG | - | Chr7:9748816-9748835 | None:intergenic | 25.0% |
| !! | TGAATTGGATTAATGGTTTT+GGG | - | Chr7:9748009-9748028 | None:intergenic | 25.0% |
| !! | TTCAATATTGTTGTTCCATT+TGG | - | Chr7:9748571-9748590 | None:intergenic | 25.0% |
| !! | TTGAAAGGTAGTATTGAATT+TGG | + | Chr7:9749086-9749105 | MsG0780036556.01.T01:CDS | 25.0% |
| !! | TTGAATTGGATTAATGGTTT+TGG | - | Chr7:9748010-9748029 | None:intergenic | 25.0% |
| !!! | AAAAACCATTTTTCGGAAAA+AGG | - | Chr7:9748204-9748223 | None:intergenic | 25.0% |
| !!! | AAAACCATTTTTCGGAAAAA+GGG | - | Chr7:9748203-9748222 | None:intergenic | 25.0% |
| !!! | TATGAGATTGATTTTGGTTT+TGG | + | Chr7:9748993-9749012 | MsG0780036556.01.T01:CDS | 25.0% |
| !!! | TATTAGAACCATTTTGGAAA+GGG | + | Chr7:9748347-9748366 | MsG0780036556.01.T01:CDS | 25.0% |
| !!! | TCACATTTTTTGACTTGATT+TGG | + | Chr7:9747816-9747835 | MsG0780036556.01.T01:CDS | 25.0% |
| !!! | TCTAATAAAGGCTCTAAATT+TGG | - | Chr7:9748334-9748353 | None:intergenic | 25.0% |
| !!! | TTATTAGAACCATTTTGGAA+AGG | + | Chr7:9748346-9748365 | MsG0780036556.01.T01:CDS | 25.0% |
| !!! | TTCAAACCATTTTTCATGAA+GGG | + | Chr7:9749141-9749160 | MsG0780036556.01.T01:CDS | 25.0% |
| !!! | TTCCAAAATGGTTCTAATAA+AGG | - | Chr7:9748346-9748365 | None:intergenic | 25.0% |
| !!! | TTTCAAACCATTTTTCATGA+AGG | + | Chr7:9749140-9749159 | MsG0780036556.01.T01:CDS | 25.0% |
| ACAGAAAGTAAGGATTTGAA+AGG | + | Chr7:9749071-9749090 | MsG0780036556.01.T01:CDS | 30.0% | |
| CAAAACAAAAGAGATGGATA+AGG | + | Chr7:9748869-9748888 | MsG0780036556.01.T01:CDS | 30.0% | |
| CAATTCAATCCAAATGATGA+CGG | + | Chr7:9748022-9748041 | MsG0780036556.01.T01:CDS | 30.0% | |
| GAGATTACTTCAATTGATAG+AGG | + | Chr7:9749032-9749051 | MsG0780036556.01.T01:CDS | 30.0% | |
| TAGAAGAAAATACGTTCTAC+TGG | - | Chr7:9747854-9747873 | None:intergenic | 30.0% | |
| TATATAGCTATGATTGGTGA+AGG | + | Chr7:9748927-9748946 | MsG0780036556.01.T01:CDS | 30.0% | |
| TCAAGTCAAAAAATGTGAGA+AGG | - | Chr7:9747814-9747833 | None:intergenic | 30.0% | |
| TGGAACAACAATATTGAAGA+AGG | + | Chr7:9748573-9748592 | MsG0780036556.01.T01:CDS | 30.0% | |
| ! | AGCCTTTATTAGAACCATTT+TGG | + | Chr7:9748341-9748360 | MsG0780036556.01.T01:CDS | 30.0% |
| ! | ATAACCCTTTTTCCGAAAAA+TGG | + | Chr7:9748196-9748215 | MsG0780036556.01.T01:CDS | 30.0% |
| ! | TGTTTCCTTCGATCAAATTT+TGG | + | Chr7:9748081-9748100 | MsG0780036556.01.T01:CDS | 30.0% |
| !! | AATTGGTTTGACAGAAAGTA+AGG | + | Chr7:9749061-9749080 | MsG0780036556.01.T01:CDS | 30.0% |
| !! | CCAATGGAAAAACCATTTTT+CGG | - | Chr7:9748211-9748230 | None:intergenic | 30.0% |
| !! | GAATTGGATTAATGGTTTTG+GGG | - | Chr7:9748008-9748027 | None:intergenic | 30.0% |
| !! | GGAGTATATGAGATTGATTT+TGG | + | Chr7:9748987-9749006 | MsG0780036556.01.T01:CDS | 30.0% |
| !!! | AGATTGATTTTGGTTTTGGA+AGG | + | Chr7:9748997-9749016 | MsG0780036556.01.T01:CDS | 30.0% |
| !!! | ATTTTCTTTTGGTTTTCCTG+TGG | + | Chr7:9748710-9748729 | MsG0780036556.01.T01:CDS | 30.0% |
| !!! | TTTCTGCTTTTTATCGCTTT+CGG | - | Chr7:9748691-9748710 | None:intergenic | 30.0% |
| AAACGATGTTACCAGCTAAA+GGG | - | Chr7:9747973-9747992 | None:intergenic | 35.0% | |
| AGCACATGTAAGAACAAAAG+TGG | - | Chr7:9748631-9748650 | None:intergenic | 35.0% | |
| ATGATTGGTGAAGGAGTTAA+AGG | + | Chr7:9748936-9748955 | MsG0780036556.01.T01:CDS | 35.0% | |
| CGTCATCATTTGGATTGAAT+TGG | - | Chr7:9748024-9748043 | None:intergenic | 35.0% | |
| GAATCTGATGATTCTAAGTG+AGG | - | Chr7:9748151-9748170 | None:intergenic | 35.0% | |
| GAGAAACTTTGCATTGTTCA+TGG | - | Chr7:9747766-9747785 | None:intergenic | 35.0% | |
| TGCATTTCAACCAATCGTTA+AGG | + | Chr7:9748479-9748498 | MsG0780036556.01.T01:intron | 35.0% | |
| TGTTCTTGATAAGCATGTGA+TGG | + | Chr7:9749112-9749131 | MsG0780036556.01.T01:CDS | 35.0% | |
| TGTTTCCGTTTGTATAGCAA+AGG | + | Chr7:9748653-9748672 | MsG0780036556.01.T01:CDS | 35.0% | |
| TTAAAGGAATAGGAGTTGCA+GGG | + | Chr7:9748952-9748971 | MsG0780036556.01.T01:CDS | 35.0% | |
| ! | ATAACAGGGTGTTATCCAAA+TGG | + | Chr7:9748553-9748572 | MsG0780036556.01.T01:intron | 35.0% |
| ! | ATGGATAAGGGTTTTCTTGA+TGG | + | Chr7:9748882-9748901 | MsG0780036556.01.T01:CDS | 35.0% |
| ! | ATTTTCCATCAAGAACAGCA+TGG | - | Chr7:9748246-9748265 | None:intergenic | 35.0% |
| ! | CCGAAAAATGGTTTTTCCAT+TGG | + | Chr7:9748208-9748227 | MsG0780036556.01.T01:CDS | 35.0% |
| ! | TTTTATCGCTTTCGGATTGT+TGG | - | Chr7:9748683-9748702 | None:intergenic | 35.0% |
| !! | GATAGTTGGGTTGAAGAATT+AGG | - | Chr7:9747788-9747807 | None:intergenic | 35.0% |
| AAGGAAACTCCGTCATCATT+TGG | - | Chr7:9748034-9748053 | None:intergenic | 40.0% | |
| AATCTTGATCTGCAATCCAC+AGG | - | Chr7:9748729-9748748 | None:intergenic | 40.0% | |
| ACAAAAGTGGACAGCTTCTT+AGG | - | Chr7:9748618-9748637 | None:intergenic | 40.0% | |
| AGACCATGCTTTGATGAACA+TGG | - | Chr7:9748275-9748294 | None:intergenic | 40.0% | |
| ATGGTGTGTGCTTATTCCAA+TGG | - | Chr7:9748227-9748246 | None:intergenic | 40.0% | |
| ATGTGAGAAGGAGAGATAGT+TGG | - | Chr7:9747802-9747821 | None:intergenic | 40.0% | |
| CAAACGATGTTACCAGCTAA+AGG | - | Chr7:9747974-9747993 | None:intergenic | 40.0% | |
| CAACCATGTTCATCAAAGCA+TGG | + | Chr7:9748269-9748288 | MsG0780036556.01.T01:CDS | 40.0% | |
| CGAACTGAATCCTTAACGAT+TGG | - | Chr7:9748492-9748511 | None:intergenic | 40.0% | |
| CTTTAGCTGGTAACATCGTT+TGG | + | Chr7:9747972-9747991 | MsG0780036556.01.T01:CDS | 40.0% | |
| GATGACTTCCCTTTCCAAAA+TGG | - | Chr7:9748358-9748377 | None:intergenic | 40.0% | |
| GGTGAAGGAGTTAAAGGAAT+AGG | + | Chr7:9748942-9748961 | MsG0780036556.01.T01:CDS | 40.0% | |
| GTGCTCCAAAATTTGATCGA+AGG | - | Chr7:9748089-9748108 | None:intergenic | 40.0% | |
| GTTAAAGGAATAGGAGTTGC+AGG | + | Chr7:9748951-9748970 | MsG0780036556.01.T01:CDS | 40.0% | |
| TAGGGTGTGAATGTGAACTT+GGG | - | Chr7:9747883-9747902 | None:intergenic | 40.0% | |
| TGTGAGAAGGAGAGATAGTT+GGG | - | Chr7:9747801-9747820 | None:intergenic | 40.0% | |
| ! | GATCGCCTTTGCTATACAAA+CGG | - | Chr7:9748661-9748680 | None:intergenic | 40.0% |
| !! | GAAGTAATCTCCACTTTTGC+AGG | - | Chr7:9749023-9749042 | None:intergenic | 40.0% |
| !! | GAGAGCGAAGATTTGAGTTT+TGG | - | Chr7:9747929-9747948 | None:intergenic | 40.0% |
| !!! | AATGGTTTTGGGGAATCAGA+AGG | - | Chr7:9747998-9748017 | None:intergenic | 40.0% |
| ACACACCATGCTGTTCTTGA+TGG | + | Chr7:9748238-9748257 | MsG0780036556.01.T01:CDS | 45.0% | |
| CGAGACAAAGAAGCTTCTTG+TGG | - | Chr7:9748118-9748137 | None:intergenic | 45.0% | |
| GTAGGGTGTGAATGTGAACT+TGG | - | Chr7:9747884-9747903 | None:intergenic | 45.0% | |
| GTCACATTGTGCTAAGAGCA+AGG | - | Chr7:9748053-9748072 | None:intergenic | 45.0% | |
| GTGTGAATGTGAACTTGGGA+GGG | - | Chr7:9747879-9747898 | None:intergenic | 45.0% | |
| !! | TTTTGGAAGGCCTGCAAAAG+TGG | + | Chr7:9749010-9749029 | MsG0780036556.01.T01:CDS | 45.0% |
| CAACACTTCCTCCCTTTAGC+TGG | + | Chr7:9747959-9747978 | MsG0780036556.01.T01:CDS | 50.0% | |
| CGATGTTACCAGCTAAAGGG+AGG | - | Chr7:9747970-9747989 | None:intergenic | 50.0% | |
| GGTGTGAATGTGAACTTGGG+AGG | - | Chr7:9747880-9747899 | None:intergenic | 50.0% | |
| GTTGCAGGGTCAACAAGGTT+TGG | + | Chr7:9748966-9748985 | MsG0780036556.01.T01:CDS | 50.0% | |
| TAGGAGTTGCAGGGTCAACA+AGG | + | Chr7:9748961-9748980 | MsG0780036556.01.T01:CDS | 50.0% | |
| ! | CAGCTAAAGGGAGGAAGTGT+TGG | - | Chr7:9747961-9747980 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 9747734 | 9749181 | 9747734 | ID=MsG0780036556.01;Name=MsG0780036556.01 |
| Chr7 | mRNA | 9747734 | 9749181 | 9747734 | ID=MsG0780036556.01.T01;Parent=MsG0780036556.01;Name=MsG0780036556.01.T01;_AED=0.34;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|446 |
| Chr7 | exon | 9747734 | 9748452 | 9747734 | ID=MsG0780036556.01.T01:exon:10323;Parent=MsG0780036556.01.T01 |
| Chr7 | exon | 9748560 | 9749181 | 9748560 | ID=MsG0780036556.01.T01:exon:10324;Parent=MsG0780036556.01.T01 |
| Chr7 | CDS | 9747734 | 9748452 | 9747734 | ID=MsG0780036556.01.T01:cds;Parent=MsG0780036556.01.T01 |
| Chr7 | CDS | 9748560 | 9749181 | 9748560 | ID=MsG0780036556.01.T01:cds;Parent=MsG0780036556.01.T01 |
| Gene Sequence |
| Protein sequence |
