Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036562.01.T01 | XP_039684187.1 | 91.25 | 480 | 42 | 0 | 1 | 480 | 1 | 480 | 0 | 899 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036562.01.T01 | Q940Z5 | 39.085 | 481 | 259 | 15 | 8 | 477 | 10 | 467 | 5.55E-92 | 290 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036562.01.T01 | A0A396H0H8 | 91.250 | 480 | 42 | 0 | 1 | 480 | 1 | 480 | 0.0 | 899 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004458.01 | MsG0780036562.01 | 0.803132 | 4.034449e-49 | 2.011430e-46 |
| MsG0780036562.01 | MsG0880045755.01 | 0.803708 | 3.063755e-49 | 1.550270e-46 |
| MsG0780036562.01 | MsG0880047251.01 | 0.812375 | 4.376705e-51 | 2.778025e-48 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036562.01.T01 | MTR_7g013850 | 76.398 | 483 | 111 | 2 | 1 | 480 | 1 | 483 | 0.0 | 758 |
| MsG0780036562.01.T01 | MTR_7g014200 | 59.006 | 483 | 177 | 11 | 1 | 477 | 1 | 468 | 0.0 | 544 |
| MsG0780036562.01.T01 | MTR_7g014160 | 56.965 | 481 | 192 | 10 | 5 | 480 | 6 | 476 | 0.0 | 535 |
| MsG0780036562.01.T01 | MTR_7g014610 | 58.351 | 485 | 186 | 12 | 1 | 478 | 1 | 476 | 0.0 | 517 |
| MsG0780036562.01.T01 | MTR_7g014620 | 51.398 | 465 | 188 | 9 | 19 | 480 | 6 | 435 | 6.47e-149 | 432 |
| MsG0780036562.01.T01 | MTR_7g014630 | 49.593 | 369 | 161 | 8 | 6 | 361 | 7 | 363 | 2.19e-110 | 331 |
| MsG0780036562.01.T01 | MTR_6g015320 | 39.709 | 481 | 259 | 10 | 1 | 477 | 1 | 454 | 1.42e-108 | 330 |
| MsG0780036562.01.T01 | MTR_6g015315 | 39.789 | 475 | 261 | 10 | 4 | 477 | 2 | 452 | 2.03e-107 | 327 |
| MsG0780036562.01.T01 | MTR_6g015815 | 40.685 | 467 | 236 | 10 | 20 | 477 | 26 | 460 | 4.74e-102 | 313 |
| MsG0780036562.01.T01 | MTR_6g015850 | 40.535 | 486 | 251 | 13 | 1 | 477 | 1 | 457 | 1.83e-101 | 311 |
| MsG0780036562.01.T01 | MTR_6g015830 | 40.956 | 481 | 243 | 14 | 9 | 477 | 9 | 460 | 2.66e-101 | 311 |
| MsG0780036562.01.T01 | MTR_7g014330 | 38.669 | 481 | 256 | 15 | 9 | 477 | 3 | 456 | 5.14e-100 | 308 |
| MsG0780036562.01.T01 | MTR_7g014230 | 38.155 | 477 | 262 | 11 | 9 | 476 | 5 | 457 | 2.09e-99 | 306 |
| MsG0780036562.01.T01 | MTR_7g014250 | 38.174 | 482 | 257 | 14 | 9 | 477 | 3 | 456 | 9.10e-99 | 305 |
| MsG0780036562.01.T01 | MTR_3g028210 | 35.331 | 484 | 272 | 15 | 8 | 477 | 8 | 464 | 6.48e-78 | 251 |
| MsG0780036562.01.T01 | MTR_7g014180 | 68.715 | 179 | 49 | 5 | 1 | 178 | 1 | 173 | 1.04e-74 | 233 |
| MsG0780036562.01.T01 | MTR_2g089765 | 31.301 | 492 | 283 | 15 | 11 | 477 | 21 | 482 | 1.92e-67 | 224 |
| MsG0780036562.01.T01 | MTR_7g014360 | 33.252 | 409 | 223 | 12 | 76 | 477 | 21 | 386 | 9.02e-67 | 219 |
| MsG0780036562.01.T01 | MTR_7g014670 | 54.737 | 190 | 84 | 2 | 289 | 477 | 71 | 259 | 8.28e-65 | 210 |
| MsG0780036562.01.T01 | MTR_7g015860 | 32.041 | 490 | 271 | 15 | 8 | 473 | 5 | 456 | 1.14e-61 | 208 |
| MsG0780036562.01.T01 | MTR_2g089670 | 31.398 | 465 | 276 | 16 | 26 | 477 | 43 | 477 | 2.51e-61 | 208 |
| MsG0780036562.01.T01 | MTR_3g087640 | 30.879 | 489 | 292 | 15 | 9 | 477 | 10 | 472 | 5.29e-61 | 207 |
| MsG0780036562.01.T01 | MTR_2g089650 | 29.485 | 485 | 297 | 12 | 8 | 477 | 9 | 463 | 1.10e-60 | 206 |
| MsG0780036562.01.T01 | MTR_2g089755 | 30.258 | 466 | 282 | 13 | 28 | 477 | 37 | 475 | 3.18e-58 | 199 |
| MsG0780036562.01.T01 | MTR_2g089735 | 31.473 | 448 | 271 | 11 | 41 | 477 | 39 | 461 | 3.13e-57 | 197 |
| MsG0780036562.01.T01 | MTR_8g012750 | 30.917 | 469 | 283 | 16 | 26 | 475 | 24 | 470 | 1.86e-55 | 192 |
| MsG0780036562.01.T01 | MTR_6g015310 | 31.358 | 405 | 205 | 10 | 51 | 455 | 2 | 333 | 3.01e-54 | 185 |
| MsG0780036562.01.T01 | MTR_7g014310 | 27.027 | 333 | 174 | 8 | 130 | 460 | 41 | 306 | 1.08e-33 | 129 |
| MsG0780036562.01.T01 | MTR_2g089665 | 25.771 | 454 | 202 | 16 | 11 | 454 | 7 | 335 | 5.14e-27 | 111 |
| MsG0780036562.01.T01 | MTR_3g435300 | 31.624 | 234 | 135 | 6 | 7 | 229 | 3 | 222 | 5.41e-27 | 110 |
| MsG0780036562.01.T01 | MTR_4g007540 | 23.299 | 485 | 300 | 20 | 8 | 475 | 3 | 432 | 4.85e-23 | 101 |
| MsG0780036562.01.T01 | MTR_8g075610 | 23.810 | 378 | 223 | 13 | 67 | 429 | 47 | 374 | 2.59e-21 | 96.3 |
| MsG0780036562.01.T01 | MTR_6g045553 | 24.516 | 465 | 275 | 20 | 7 | 439 | 13 | 433 | 4.04e-21 | 96.3 |
| MsG0780036562.01.T01 | MTR_3g101900 | 23.841 | 453 | 285 | 16 | 28 | 467 | 30 | 435 | 3.05e-20 | 93.6 |
| MsG0780036562.01.T01 | MTR_2g064495 | 22.271 | 458 | 290 | 19 | 31 | 475 | 27 | 431 | 3.21e-19 | 90.1 |
| MsG0780036562.01.T01 | MTR_7g086000 | 25.445 | 393 | 219 | 15 | 67 | 429 | 60 | 408 | 4.11e-19 | 90.1 |
| MsG0780036562.01.T01 | MTR_2g105330 | 22.489 | 458 | 289 | 19 | 31 | 475 | 27 | 431 | 6.68e-19 | 89.4 |
| MsG0780036562.01.T01 | MTR_3g049150 | 23.992 | 496 | 301 | 21 | 8 | 477 | 2 | 447 | 4.35e-18 | 87.0 |
| MsG0780036562.01.T01 | MTR_3g026310 | 23.516 | 438 | 282 | 18 | 17 | 441 | 14 | 411 | 5.98e-18 | 86.7 |
| MsG0780036562.01.T01 | MTR_4g094835 | 24.537 | 432 | 248 | 18 | 28 | 439 | 32 | 405 | 1.50e-17 | 85.1 |
| MsG0780036562.01.T01 | MTR_4g094842 | 24.500 | 400 | 231 | 16 | 62 | 439 | 57 | 407 | 9.50e-17 | 82.8 |
| MsG0780036562.01.T01 | MTR_4g097090 | 24.408 | 422 | 257 | 19 | 26 | 428 | 28 | 406 | 1.95e-16 | 82.0 |
| MsG0780036562.01.T01 | MTR_3g101930 | 23.789 | 475 | 298 | 20 | 13 | 467 | 12 | 442 | 3.06e-16 | 81.3 |
| MsG0780036562.01.T01 | MTR_2g007880 | 24.459 | 462 | 239 | 21 | 50 | 470 | 48 | 440 | 3.09e-16 | 81.3 |
| MsG0780036562.01.T01 | MTR_8g096530 | 24.537 | 432 | 262 | 16 | 7 | 429 | 6 | 382 | 7.40e-16 | 80.1 |
| MsG0780036562.01.T01 | MTR_2g015430 | 20.172 | 466 | 286 | 14 | 8 | 456 | 2 | 398 | 9.26e-16 | 79.7 |
| MsG0780036562.01.T01 | MTR_7g026860 | 25.485 | 412 | 259 | 16 | 25 | 430 | 21 | 390 | 3.73e-15 | 77.8 |
| MsG0780036562.01.T01 | MTR_7g014210 | 33.645 | 107 | 69 | 2 | 335 | 439 | 24 | 130 | 5.29e-15 | 73.2 |
| MsG0780036562.01.T01 | MTR_7g085930 | 23.517 | 472 | 287 | 21 | 36 | 477 | 34 | 461 | 6.87e-15 | 77.0 |
| MsG0780036562.01.T01 | MTR_8g467580 | 23.077 | 481 | 266 | 18 | 17 | 466 | 15 | 422 | 1.42e-14 | 76.3 |
| MsG0780036562.01.T01 | MTR_0190s0020 | 24.586 | 423 | 264 | 16 | 25 | 439 | 26 | 401 | 4.11e-14 | 74.7 |
| MsG0780036562.01.T01 | MTR_3g098980 | 25.260 | 289 | 172 | 11 | 67 | 352 | 66 | 313 | 1.15e-13 | 73.2 |
| MsG0780036562.01.T01 | MTR_4g094832 | 26.871 | 294 | 173 | 13 | 150 | 439 | 134 | 389 | 1.33e-13 | 73.2 |
| MsG0780036562.01.T01 | MTR_4g022290 | 22.034 | 472 | 284 | 16 | 19 | 467 | 22 | 432 | 1.76e-13 | 72.8 |
| MsG0780036562.01.T01 | MTR_7g014205 | 29.032 | 124 | 76 | 2 | 317 | 439 | 2 | 114 | 1.92e-13 | 67.4 |
| MsG0780036562.01.T01 | MTR_4g022670 | 24.944 | 445 | 262 | 19 | 17 | 442 | 14 | 405 | 3.16e-13 | 72.0 |
| MsG0780036562.01.T01 | MTR_8g079980 | 23.438 | 448 | 274 | 16 | 17 | 453 | 15 | 404 | 3.86e-13 | 71.6 |
| MsG0780036562.01.T01 | MTR_7g014340 | 54.930 | 71 | 25 | 2 | 9 | 75 | 3 | 70 | 5.85e-13 | 65.1 |
| MsG0780036562.01.T01 | MTR_7g084940 | 22.164 | 379 | 236 | 12 | 61 | 429 | 63 | 392 | 6.86e-13 | 70.9 |
| MsG0780036562.01.T01 | MTR_4g015180 | 21.830 | 481 | 337 | 15 | 10 | 466 | 8 | 473 | 7.72e-13 | 70.9 |
| MsG0780036562.01.T01 | MTR_7g014220 | 60.377 | 53 | 18 | 1 | 25 | 77 | 15 | 64 | 5.31e-12 | 62.0 |
| MsG0780036562.01.T01 | MTR_4g055170 | 22.951 | 366 | 240 | 10 | 67 | 426 | 69 | 398 | 7.71e-12 | 67.8 |
| MsG0780036562.01.T01 | MTR_4g022760 | 25.348 | 359 | 218 | 15 | 17 | 369 | 14 | 328 | 9.39e-12 | 67.0 |
| MsG0780036562.01.T01 | MTR_8g467590 | 25.377 | 398 | 226 | 20 | 60 | 442 | 53 | 394 | 1.23e-11 | 67.0 |
| MsG0780036562.01.T01 | MTR_3g435290 | 44.776 | 67 | 35 | 1 | 411 | 477 | 53 | 117 | 5.12e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036562.01.T01 | AT5G39050 | 39.085 | 481 | 259 | 15 | 8 | 477 | 10 | 467 | 5.66e-93 | 290 |
| MsG0780036562.01.T01 | AT3G29590 | 36.673 | 499 | 243 | 12 | 1 | 477 | 1 | 448 | 1.47e-92 | 288 |
| MsG0780036562.01.T01 | AT5G39080 | 38.462 | 481 | 266 | 15 | 1 | 474 | 1 | 458 | 8.36e-92 | 287 |
| MsG0780036562.01.T01 | AT3G29635 | 38.074 | 457 | 253 | 11 | 24 | 477 | 25 | 454 | 2.96e-90 | 283 |
| MsG0780036562.01.T01 | AT3G29670 | 38.622 | 479 | 253 | 15 | 8 | 477 | 3 | 449 | 1.08e-87 | 276 |
| MsG0780036562.01.T01 | AT5G39090 | 36.667 | 480 | 268 | 12 | 1 | 477 | 1 | 447 | 2.69e-87 | 275 |
| MsG0780036562.01.T01 | AT5G61160 | 34.034 | 476 | 276 | 15 | 8 | 475 | 3 | 448 | 3.17e-77 | 249 |
| MsG0780036562.01.T01 | AT3G29680 | 35.368 | 475 | 269 | 13 | 4 | 473 | 2 | 443 | 3.30e-73 | 238 |
| MsG0780036562.01.T01 | AT1G03940 | 30.000 | 500 | 291 | 15 | 1 | 477 | 1 | 464 | 1.86e-62 | 211 |
| MsG0780036562.01.T01 | AT1G03495 | 30.728 | 371 | 216 | 12 | 1 | 359 | 1 | 342 | 1.35e-40 | 149 |
| MsG0780036562.01.T01 | AT5G23940 | 26.096 | 456 | 265 | 20 | 8 | 439 | 3 | 410 | 2.34e-23 | 103 |
| MsG0780036562.01.T01 | AT2G19070 | 24.444 | 405 | 250 | 16 | 41 | 428 | 37 | 402 | 1.66e-21 | 97.4 |
| MsG0780036562.01.T01 | AT5G67150 | 23.769 | 467 | 303 | 15 | 16 | 471 | 13 | 437 | 2.47e-19 | 90.9 |
| MsG0780036562.01.T01 | AT5G48930 | 22.934 | 484 | 303 | 19 | 8 | 475 | 3 | 432 | 3.78e-19 | 90.1 |
| MsG0780036562.01.T01 | AT3G29720 | 35.429 | 175 | 82 | 7 | 135 | 303 | 52 | 201 | 1.49e-18 | 84.7 |
| MsG0780036562.01.T01 | AT3G50280 | 23.709 | 426 | 269 | 15 | 19 | 434 | 18 | 397 | 5.44e-18 | 86.7 |
| MsG0780036562.01.T01 | AT5G07850 | 24.834 | 451 | 277 | 19 | 1 | 434 | 1 | 406 | 1.31e-17 | 85.5 |
| MsG0780036562.01.T01 | AT2G39980 | 23.469 | 490 | 327 | 14 | 1 | 466 | 1 | 466 | 1.08e-16 | 82.8 |
| MsG0780036562.01.T01 | AT3G29690 | 31.788 | 151 | 97 | 2 | 328 | 477 | 33 | 178 | 1.60e-16 | 77.8 |
| MsG0780036562.01.T01 | AT4G31910 | 22.626 | 358 | 230 | 9 | 44 | 389 | 46 | 368 | 2.50e-14 | 75.5 |
| MsG0780036562.01.T01 | AT1G28680 | 20.699 | 372 | 245 | 11 | 67 | 432 | 62 | 389 | 1.12e-13 | 73.6 |
| MsG0780036562.01.T01 | AT5G41040 | 22.667 | 375 | 241 | 14 | 61 | 429 | 63 | 394 | 3.80e-13 | 71.6 |
| MsG0780036562.01.T01 | AT5G41040 | 22.764 | 369 | 236 | 14 | 67 | 429 | 85 | 410 | 6.65e-13 | 70.9 |
| MsG0780036562.01.T01 | AT3G47170 | 23.344 | 317 | 202 | 11 | 55 | 365 | 65 | 346 | 7.47e-11 | 64.7 |
Find 59 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTACTTCCCTACTCAAAA+AGG | 0.196644 | 7:-9846050 | None:intergenic |
| GTAGGGTGTGATTGTGAATT+TGG | 0.209360 | 7:-9845575 | None:intergenic |
| GAGAGTGAAGATTTGAGTTT+TGG | 0.216722 | 7:-9845620 | None:intergenic |
| GATAATTGGTTTGCAGAATT+AGG | 0.230849 | 7:-9845479 | None:intergenic |
| TTGAAAGGTGGTGTTGAATT+TGG | 0.233566 | 7:+9846778 | MsG0780036562.01.T01:CDS |
| ACAAAAGTTGACAATTTCTT+AGG | 0.235091 | 7:-9846307 | None:intergenic |
| TCTTCCTTTGTAAAGTCTTC+TGG | 0.249623 | 7:-9846505 | None:intergenic |
| GGAGTATATGAGATTGATTT+TGG | 0.276059 | 7:+9846679 | MsG0780036562.01.T01:CDS |
| CCAATTTCCGAAAACTATTT+CGG | 0.280907 | 7:+9846448 | MsG0780036562.01.T01:CDS |
| AAGTGAGAGGAAGAGATAAT+TGG | 0.284573 | 7:-9845493 | None:intergenic |
| CGAACGGAATCCTTAACTTT+TGG | 0.284718 | 7:-9846184 | None:intergenic |
| TTCTTCAATATTGTTCCATT+TGG | 0.285958 | 7:-9846263 | None:intergenic |
| TTGAACTGAATGATTGGTTT+TGG | 0.292722 | 7:-9845701 | None:intergenic |
| CGAGCTCACACGTGAAGATT+TGG | 0.321526 | 7:+9846216 | MsG0780036562.01.T01:CDS |
| AACGAAACTCCGTCATCATT+TGG | 0.340462 | 7:-9845725 | None:intergenic |
| TCACTTTCTTAGACTTGAAT+TGG | 0.344769 | 7:+9845510 | MsG0780036562.01.T01:CDS |
| CTATAGCAGGTAAGATTGTT+TGG | 0.361427 | 7:+9845666 | MsG0780036562.01.T01:CDS |
| TAGAAGAAAATACGTTCAAC+TGG | 0.370556 | 7:-9845545 | None:intergenic |
| CAAACAATCTTACCTGCTAT+AGG | 0.371223 | 7:-9845665 | None:intergenic |
| GTTGATAGAGGAATAACAAT+TGG | 0.386783 | 7:+9846736 | MsG0780036562.01.T01:CDS |
| ATCTCACTCTCAAAACCATT+TGG | 0.401328 | 7:-9846079 | None:intergenic |
| ATTCAAGCAATAGGAGTTGC+AGG | 0.408252 | 7:+9846643 | MsG0780036562.01.T01:CDS |
| TAGGGTGTGATTGTGAATTT+GGG | 0.408902 | 7:-9845574 | None:intergenic |
| TTTGGATTGAACTGAATGAT+TGG | 0.409903 | 7:-9845707 | None:intergenic |
| TCTAGATATAAGGATATTGT+TGG | 0.420578 | 7:+9846613 | MsG0780036562.01.T01:CDS |
| ACACACCATGCTGTTCTTGA+TGG | 0.421878 | 7:+9845932 | MsG0780036562.01.T01:CDS |
| AAACAATCTTACCTGCTATA+GGG | 0.427153 | 7:-9845664 | None:intergenic |
| ACAAAAGTGTGTTGTCCAAA+TGG | 0.432381 | 7:+9846248 | MsG0780036562.01.T01:CDS |
| TTTGAGTAGGGAAGTAATCA+AGG | 0.453618 | 7:+9846054 | MsG0780036562.01.T01:CDS |
| GGTGAAGGAATTCAAGCAAT+AGG | 0.454785 | 7:+9846634 | MsG0780036562.01.T01:CDS |
| GTTGCAGGGTCAACAAGGTT+TGG | 0.454958 | 7:+9846658 | MsG0780036562.01.T01:CDS |
| AGAGTGAGATCATAAATAGT+TGG | 0.463701 | 7:+9846092 | MsG0780036562.01.T01:CDS |
| CTGCTATAGGGAGAAAGTGT+TGG | 0.519539 | 7:-9845652 | None:intergenic |
| GAAAGTAGAGATTTGAAAGG+TGG | 0.519737 | 7:+9846766 | MsG0780036562.01.T01:CDS |
| AAATGGAACAATATTGAAGA+AGG | 0.523989 | 7:+9846265 | MsG0780036562.01.T01:CDS |
| GAAGTAATCAAGGATCCAAA+TGG | 0.526584 | 7:+9846064 | MsG0780036562.01.T01:CDS |
| GAAGACTTTACAAAGGAAGA+TGG | 0.532610 | 7:+9846508 | MsG0780036562.01.T01:CDS |
| CTATAGGGAGAAAGTGTTGG+AGG | 0.537643 | 7:-9845649 | None:intergenic |
| GAAACTACTTCAGTTGATAG+AGG | 0.550153 | 7:+9846724 | MsG0780036562.01.T01:CDS |
| CAACACTTTCTCCCTATAGC+AGG | 0.551304 | 7:+9845653 | MsG0780036562.01.T01:CDS |
| AAATGATGACGGAGTTTCGT+TGG | 0.551389 | 7:+9845727 | MsG0780036562.01.T01:CDS |
| TATAAGGATATTGTTGGTGA+AGG | 0.559361 | 7:+9846619 | MsG0780036562.01.T01:CDS |
| AGTTTCCTTCGATAAAATTG+TGG | 0.567981 | 7:+9845775 | MsG0780036562.01.T01:CDS |
| ACAGAAAGTAGAGATTTGAA+AGG | 0.576880 | 7:+9846763 | MsG0780036562.01.T01:CDS |
| TGTGATTGTGAATTTGGGAG+AGG | 0.582568 | 7:-9845569 | None:intergenic |
| GTGATTGTGAATTTGGGAGA+GGG | 0.585407 | 7:-9845568 | None:intergenic |
| TTCAAGTCTAAGAAAGTGAG+AGG | 0.591765 | 7:-9845506 | None:intergenic |
| CGAGATAAATAAGCTTCTTG+TGG | 0.592058 | 7:-9845809 | None:intergenic |
| GAATCTGTTGATTCTAAGTG+AGG | 0.596592 | 7:-9845842 | None:intergenic |
| CAGTTCAATCCAAATGATGA+CGG | 0.611704 | 7:+9845716 | MsG0780036562.01.T01:CDS |
| TAGGAGTTGCAGGGTCAACA+AGG | 0.619039 | 7:+9846653 | MsG0780036562.01.T01:CDS |
| CAACCATGTTCATCAAAGCA+TGG | 0.631200 | 7:+9845963 | MsG0780036562.01.T01:CDS |
| ATGGTGTGTGCTTATTCCAA+TGG | 0.644576 | 7:-9845918 | None:intergenic |
| GAGCTCGAATGCGACTCGAA+CGG | 0.653104 | 7:-9846200 | None:intergenic |
| AGACCATGCTTTGATGAACA+TGG | 0.653845 | 7:-9845966 | None:intergenic |
| TATAGGGAGAAAGTGTTGGA+GGG | 0.656814 | 7:-9845648 | None:intergenic |
| TTCAAGCAATAGGAGTTGCA+GGG | 0.658447 | 7:+9846644 | MsG0780036562.01.T01:CDS |
| TGAGCCAGAAGACTTTACAA+AGG | 0.666705 | 7:+9846501 | MsG0780036562.01.T01:CDS |
| AGAAGAAAATACGTTCAACT+GGG | 0.676059 | 7:-9845544 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATTAAAAAAATGGATAA+GGG | + | Chr7:9846562-9846581 | MsG0780036562.01.T01:CDS | 15.0% |
| !! | TTATAGCACAATTAAAAAAA+TGG | + | Chr7:9846555-9846574 | MsG0780036562.01.T01:CDS | 15.0% |
| !!! | CAAAAACATAAATTTTGTTT+TGG | + | Chr7:9846391-9846410 | MsG0780036562.01.T01:CDS | 15.0% |
| !!! | TACATTTTTATCTAGATATA+AGG | + | Chr7:9846603-9846622 | MsG0780036562.01.T01:CDS | 15.0% |
| !! | CACAATTAAAAAAATGGATA+AGG | + | Chr7:9846561-9846580 | MsG0780036562.01.T01:CDS | 20.0% |
| !!! | TTTTTTTGAACCAAAAGTTA+AGG | + | Chr7:9846174-9846193 | MsG0780036562.01.T01:CDS | 20.0% |
| ! | AAATGGAACAATATTGAAGA+AGG | + | Chr7:9846265-9846284 | MsG0780036562.01.T01:CDS | 25.0% |
| ! | ACAAAAGTTGACAATTTCTT+AGG | - | Chr7:9846310-9846329 | None:intergenic | 25.0% |
| ! | TCTAGATATAAGGATATTGT+TGG | + | Chr7:9846613-9846632 | MsG0780036562.01.T01:CDS | 25.0% |
| ! | TTCTTCAATATTGTTCCATT+TGG | - | Chr7:9846266-9846285 | None:intergenic | 25.0% |
| !! | ACAATTTCCGAAATAGTTTT+CGG | - | Chr7:9846458-9846477 | None:intergenic | 25.0% |
| !!! | TATGAGATTGATTTTGGTTT+TGG | + | Chr7:9846685-9846704 | MsG0780036562.01.T01:CDS | 25.0% |
| !!! | TTCATACCATTTTTCATGAA+GGG | + | Chr7:9846833-9846852 | MsG0780036562.01.T01:CDS | 25.0% |
| !!! | TTTCATACCATTTTTCATGA+AGG | + | Chr7:9846832-9846851 | MsG0780036562.01.T01:CDS | 25.0% |
| AAACAATCTTACCTGCTATA+GGG | - | Chr7:9845667-9845686 | None:intergenic | 30.0% | |
| ACAGAAAGTAGAGATTTGAA+AGG | + | Chr7:9846763-9846782 | MsG0780036562.01.T01:CDS | 30.0% | |
| AGAAGAAAATACGTTCAACT+GGG | - | Chr7:9845547-9845566 | None:intergenic | 30.0% | |
| AGAGTGAGATCATAAATAGT+TGG | + | Chr7:9846092-9846111 | MsG0780036562.01.T01:CDS | 30.0% | |
| AGTTTCCTTCGATAAAATTG+TGG | + | Chr7:9845775-9845794 | MsG0780036562.01.T01:CDS | 30.0% | |
| ATTACTTCCCTACTCAAAAA+GGG | - | Chr7:9846052-9846071 | None:intergenic | 30.0% | |
| CCAATTTCCGAAAACTATTT+CGG | + | Chr7:9846448-9846467 | MsG0780036562.01.T01:CDS | 30.0% | |
| CTCAAAAAGGGTTTCAAAAA+AGG | - | Chr7:9846040-9846059 | None:intergenic | 30.0% | |
| TAGAAGAAAATACGTTCAAC+TGG | - | Chr7:9845548-9845567 | None:intergenic | 30.0% | |
| TCACTTTCTTAGACTTGAAT+TGG | + | Chr7:9845510-9845529 | MsG0780036562.01.T01:CDS | 30.0% | |
| TTTGGATTGAACTGAATGAT+TGG | - | Chr7:9845710-9845729 | None:intergenic | 30.0% | |
| ! | AAATAGTTTTCGGAAATTGG+TGG | - | Chr7:9846448-9846467 | None:intergenic | 30.0% |
| ! | AATAGTTTTCGGAAATTGGT+GGG | - | Chr7:9846447-9846466 | None:intergenic | 30.0% |
| ! | ATAACCCTTTTTCCGAAAAA+CGG | + | Chr7:9845890-9845909 | MsG0780036562.01.T01:CDS | 30.0% |
| ! | GTTGATAGAGGAATAACAAT+TGG | + | Chr7:9846736-9846755 | MsG0780036562.01.T01:CDS | 30.0% |
| ! | TATAAGGATATTGTTGGTGA+AGG | + | Chr7:9846619-9846638 | MsG0780036562.01.T01:CDS | 30.0% |
| !! | AAAAACCGTTTTTCGGAAAA+AGG | - | Chr7:9845898-9845917 | None:intergenic | 30.0% |
| !! | AAAACCGTTTTTCGGAAAAA+GGG | - | Chr7:9845897-9845916 | None:intergenic | 30.0% |
| !! | AATCTCAACACATTTTGATC+AGG | + | Chr7:9846120-9846139 | MsG0780036562.01.T01:CDS | 30.0% |
| !! | ATCTCAACACATTTTGATCA+GGG | + | Chr7:9846121-9846140 | MsG0780036562.01.T01:CDS | 30.0% |
| !! | GATAATTGGTTTGCAGAATT+AGG | - | Chr7:9845482-9845501 | None:intergenic | 30.0% |
| !! | GGAGTATATGAGATTGATTT+TGG | + | Chr7:9846679-9846698 | MsG0780036562.01.T01:CDS | 30.0% |
| !! | TTTTGCTTTTGCTATGCAAA+CGG | - | Chr7:9846353-9846372 | None:intergenic | 30.0% |
| !!! | AAGATTTGAGTTTTGGAACA+AGG | - | Chr7:9845616-9845635 | None:intergenic | 30.0% |
| !!! | AGATTGATTTTGGTTTTGGA+AGG | + | Chr7:9846689-9846708 | MsG0780036562.01.T01:CDS | 30.0% |
| !!! | AGGTTTTGAAAGAAGAAAGT+AGG | - | Chr7:9845596-9845615 | None:intergenic | 30.0% |
| !!! | ATTTTGTTTTGGTTTTGCAG+TGG | + | Chr7:9846402-9846421 | MsG0780036562.01.T01:CDS | 30.0% |
| !!! | GGTTTTGAAAGAAGAAAGTA+GGG | - | Chr7:9845595-9845614 | None:intergenic | 30.0% |
| !!! | TTGAACTGAATGATTGGTTT+TGG | - | Chr7:9845704-9845723 | None:intergenic | 30.0% |
| !!! | TTTGAAACCCTTTTTGAGTA+GGG | + | Chr7:9846042-9846061 | MsG0780036562.01.T01:CDS | 30.0% |
| !!! | TTTTGAAACCCTTTTTGAGT+AGG | + | Chr7:9846041-9846060 | MsG0780036562.01.T01:CDS | 30.0% |
| AAGTGAGAGGAAGAGATAAT+TGG | - | Chr7:9845496-9845515 | None:intergenic | 35.0% | |
| ATCTCACTCTCAAAACCATT+TGG | - | Chr7:9846082-9846101 | None:intergenic | 35.0% | |
| CAAACAATCTTACCTGCTAT+AGG | - | Chr7:9845668-9845687 | None:intergenic | 35.0% | |
| CAGTTCAATCCAAATGATGA+CGG | + | Chr7:9845716-9845735 | MsG0780036562.01.T01:CDS | 35.0% | |
| CGAGATAAATAAGCTTCTTG+TGG | - | Chr7:9845812-9845831 | None:intergenic | 35.0% | |
| CTATAGCAGGTAAGATTGTT+TGG | + | Chr7:9845666-9845685 | MsG0780036562.01.T01:CDS | 35.0% | |
| GAAAGTAGAGATTTGAAAGG+TGG | + | Chr7:9846766-9846785 | MsG0780036562.01.T01:CDS | 35.0% | |
| GAAGACTTTACAAAGGAAGA+TGG | + | Chr7:9846508-9846527 | MsG0780036562.01.T01:CDS | 35.0% | |
| GAAGTAATCAAGGATCCAAA+TGG | + | Chr7:9846064-9846083 | MsG0780036562.01.T01:CDS | 35.0% | |
| GATTACTTCCCTACTCAAAA+AGG | - | Chr7:9846053-9846072 | None:intergenic | 35.0% | |
| GCATAACCCTTCATGAAAAA+TGG | - | Chr7:9846842-9846861 | None:intergenic | 35.0% | |
| TAGGGTGTGATTGTGAATTT+GGG | - | Chr7:9845577-9845596 | None:intergenic | 35.0% | |
| TCTTCCTTTGTAAAGTCTTC+TGG | - | Chr7:9846508-9846527 | None:intergenic | 35.0% | |
| TTCAAGTCTAAGAAAGTGAG+AGG | - | Chr7:9845509-9845528 | None:intergenic | 35.0% | |
| TTTGAGTAGGGAAGTAATCA+AGG | + | Chr7:9846054-9846073 | MsG0780036562.01.T01:CDS | 35.0% | |
| ! | ACAAAAGTGTGTTGTCCAAA+TGG | + | Chr7:9846248-9846267 | MsG0780036562.01.T01:CDS | 35.0% |
| ! | ATGGATAAGGGTTTTCTTGA+TGG | + | Chr7:9846574-9846593 | MsG0780036562.01.T01:CDS | 35.0% |
| ! | ATTTTCCATCAAGAACAGCA+TGG | - | Chr7:9845940-9845959 | None:intergenic | 35.0% |
| ! | CCGAAATAGTTTTCGGAAAT+TGG | - | Chr7:9846451-9846470 | None:intergenic | 35.0% |
| ! | TTGAAAGGTGGTGTTGAATT+TGG | + | Chr7:9846778-9846797 | MsG0780036562.01.T01:CDS | 35.0% |
| !! | CCAATGGAAAAACCGTTTTT+CGG | - | Chr7:9845905-9845924 | None:intergenic | 35.0% |
| !! | GAAACTACTTCAGTTGATAG+AGG | + | Chr7:9846724-9846743 | MsG0780036562.01.T01:CDS | 35.0% |
| !! | GAATCTGTTGATTCTAAGTG+AGG | - | Chr7:9845845-9845864 | None:intergenic | 35.0% |
| !! | GAGAGTGAAGATTTGAGTTT+TGG | - | Chr7:9845623-9845642 | None:intergenic | 35.0% |
| !!! | ATTGGTTTTGGAGAATCAGA+AGG | - | Chr7:9845692-9845711 | None:intergenic | 35.0% |
| AACGAAACTCCGTCATCATT+TGG | - | Chr7:9845728-9845747 | None:intergenic | 40.0% | |
| AGACCATGCTTTGATGAACA+TGG | - | Chr7:9845969-9845988 | None:intergenic | 40.0% | |
| ATGGTGTGTGCTTATTCCAA+TGG | - | Chr7:9845921-9845940 | None:intergenic | 40.0% | |
| ATTCAAGCAATAGGAGTTGC+AGG | + | Chr7:9846643-9846662 | MsG0780036562.01.T01:CDS | 40.0% | |
| CAACCATGTTCATCAAAGCA+TGG | + | Chr7:9845963-9845982 | MsG0780036562.01.T01:CDS | 40.0% | |
| GGTGAAGGAATTCAAGCAAT+AGG | + | Chr7:9846634-9846653 | MsG0780036562.01.T01:CDS | 40.0% | |
| GTAGGGTGTGATTGTGAATT+TGG | - | Chr7:9845578-9845597 | None:intergenic | 40.0% | |
| GTGATTGTGAATTTGGGAGA+GGG | - | Chr7:9845571-9845590 | None:intergenic | 40.0% | |
| TGAGCCAGAAGACTTTACAA+AGG | + | Chr7:9846501-9846520 | MsG0780036562.01.T01:CDS | 40.0% | |
| TGTGATTGTGAATTTGGGAG+AGG | - | Chr7:9845572-9845591 | None:intergenic | 40.0% | |
| TTCAAGCAATAGGAGTTGCA+GGG | + | Chr7:9846644-9846663 | MsG0780036562.01.T01:CDS | 40.0% | |
| ! | CCGAAAAACGGTTTTTCCAT+TGG | + | Chr7:9845902-9845921 | MsG0780036562.01.T01:CDS | 40.0% |
| ! | CGAACGGAATCCTTAACTTT+TGG | - | Chr7:9846187-9846206 | None:intergenic | 40.0% |
| ! | CTGCTCCACAATTTTATCGA+AGG | - | Chr7:9845783-9845802 | None:intergenic | 40.0% |
| !! | AAATGATGACGGAGTTTCGT+TGG | + | Chr7:9845727-9845746 | MsG0780036562.01.T01:CDS | 40.0% |
| !! | GAAGTAGTTTCCACTTTTGC+AGG | - | Chr7:9846715-9846734 | None:intergenic | 40.0% |
| !! | TATAGGGAGAAAGTGTTGGA+GGG | - | Chr7:9845651-9845670 | None:intergenic | 40.0% |
| ACACACCATGCTGTTCTTGA+TGG | + | Chr7:9845932-9845951 | MsG0780036562.01.T01:CDS | 45.0% | |
| CAACACTTTCTCCCTATAGC+AGG | + | Chr7:9845653-9845672 | MsG0780036562.01.T01:CDS | 45.0% | |
| ! | CTGCTATAGGGAGAAAGTGT+TGG | - | Chr7:9845655-9845674 | None:intergenic | 45.0% |
| !! | CTATAGGGAGAAAGTGTTGG+AGG | - | Chr7:9845652-9845671 | None:intergenic | 45.0% |
| !! | TTTTGGAAGGCCTGCAAAAG+TGG | + | Chr7:9846702-9846721 | MsG0780036562.01.T01:CDS | 45.0% |
| CGAGCTCACACGTGAAGATT+TGG | + | Chr7:9846216-9846235 | MsG0780036562.01.T01:CDS | 50.0% | |
| GTTGCAGGGTCAACAAGGTT+TGG | + | Chr7:9846658-9846677 | MsG0780036562.01.T01:CDS | 50.0% | |
| TAGGAGTTGCAGGGTCAACA+AGG | + | Chr7:9846653-9846672 | MsG0780036562.01.T01:CDS | 50.0% | |
| GAGCTCGAATGCGACTCGAA+CGG | - | Chr7:9846203-9846222 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 9845428 | 9846870 | 9845428 | ID=MsG0780036562.01;Name=MsG0780036562.01 |
| Chr7 | mRNA | 9845428 | 9846870 | 9845428 | ID=MsG0780036562.01.T01;Parent=MsG0780036562.01;Name=MsG0780036562.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|480 |
| Chr7 | exon | 9845428 | 9846870 | 9845428 | ID=MsG0780036562.01.T01:exon:1534;Parent=MsG0780036562.01.T01 |
| Chr7 | CDS | 9845428 | 9846870 | 9845428 | ID=MsG0780036562.01.T01:cds;Parent=MsG0780036562.01.T01 |
| Gene Sequence |
| Protein sequence |
