Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036588.01.T01 | XP_039684078.1 | 92.521 | 468 | 35 | 0 | 1 | 468 | 1 | 468 | 0 | 892 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036588.01.T01 | Q940Z5 | 37.764 | 474 | 268 | 12 | 5 | 465 | 8 | 467 | 4.89E-93 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036588.01.T01 | B4Y0U0 | 76.282 | 468 | 108 | 2 | 1 | 465 | 1 | 468 | 0.0 | 741 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014920.01 | MsG0780036588.01 | 0.841049 | 6.012977e-58 | 8.728210e-55 |
| MsG0580025338.01 | MsG0780036588.01 | 0.801390 | 9.208108e-49 | 4.392138e-46 |
| MsG0580028560.01 | MsG0780036588.01 | 0.815945 | 7.126702e-52 | 4.982064e-49 |
| MsG0280007272.01 | MsG0780036588.01 | 0.807972 | 3.891824e-50 | 2.198123e-47 |
| MsG0280007391.01 | MsG0780036588.01 | 0.812409 | 4.301421e-51 | 2.732991e-48 |
| MsG0780036514.01 | MsG0780036588.01 | 0.805805 | 1.118119e-49 | 5.971535e-47 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036588.01.T01 | MTR_7g014200 | 76.282 | 468 | 108 | 2 | 1 | 465 | 1 | 468 | 0.0 | 741 |
| MsG0780036588.01.T01 | MTR_7g014160 | 71.125 | 471 | 126 | 4 | 4 | 466 | 6 | 474 | 0.0 | 691 |
| MsG0780036588.01.T01 | MTR_7g014610 | 73.222 | 478 | 118 | 4 | 1 | 468 | 1 | 478 | 0.0 | 689 |
| MsG0780036588.01.T01 | MTR_7g014620 | 64.080 | 451 | 136 | 5 | 19 | 466 | 6 | 433 | 0.0 | 548 |
| MsG0780036588.01.T01 | MTR_7g013850 | 55.509 | 481 | 196 | 8 | 2 | 466 | 3 | 481 | 8.35e-176 | 502 |
| MsG0780036588.01.T01 | MTR_7g014630 | 66.553 | 293 | 89 | 3 | 5 | 288 | 7 | 299 | 4.55e-131 | 384 |
| MsG0780036588.01.T01 | MTR_7g014180 | 93.642 | 173 | 11 | 0 | 1 | 173 | 1 | 173 | 9.01e-111 | 325 |
| MsG0780036588.01.T01 | MTR_6g015320 | 41.880 | 468 | 242 | 13 | 7 | 465 | 8 | 454 | 2.97e-106 | 323 |
| MsG0780036588.01.T01 | MTR_6g015315 | 40.564 | 461 | 257 | 9 | 8 | 465 | 6 | 452 | 4.90e-103 | 315 |
| MsG0780036588.01.T01 | MTR_6g015850 | 41.216 | 444 | 243 | 8 | 24 | 465 | 30 | 457 | 5.16e-103 | 315 |
| MsG0780036588.01.T01 | MTR_6g015815 | 40.529 | 454 | 250 | 9 | 17 | 465 | 22 | 460 | 3.70e-101 | 310 |
| MsG0780036588.01.T01 | MTR_6g015830 | 42.198 | 455 | 232 | 13 | 21 | 465 | 27 | 460 | 5.76e-101 | 310 |
| MsG0780036588.01.T01 | MTR_7g014230 | 41.365 | 469 | 247 | 14 | 8 | 464 | 5 | 457 | 6.31e-101 | 310 |
| MsG0780036588.01.T01 | MTR_7g014330 | 39.831 | 472 | 256 | 13 | 8 | 467 | 3 | 458 | 1.12e-98 | 304 |
| MsG0780036588.01.T01 | MTR_7g014250 | 39.355 | 465 | 264 | 11 | 8 | 465 | 3 | 456 | 2.76e-95 | 295 |
| MsG0780036588.01.T01 | MTR_7g014670 | 71.429 | 182 | 51 | 1 | 284 | 465 | 79 | 259 | 6.79e-90 | 275 |
| MsG0780036588.01.T01 | MTR_3g028210 | 33.895 | 475 | 280 | 13 | 7 | 465 | 8 | 464 | 8.47e-74 | 240 |
| MsG0780036588.01.T01 | MTR_2g089765 | 31.535 | 482 | 292 | 13 | 7 | 465 | 16 | 482 | 7.01e-67 | 222 |
| MsG0780036588.01.T01 | MTR_7g015860 | 33.058 | 484 | 277 | 12 | 4 | 465 | 2 | 460 | 1.82e-66 | 221 |
| MsG0780036588.01.T01 | MTR_7g014360 | 36.658 | 401 | 210 | 12 | 77 | 467 | 22 | 388 | 3.76e-66 | 218 |
| MsG0780036588.01.T01 | MTR_2g089735 | 33.710 | 442 | 257 | 14 | 41 | 465 | 39 | 461 | 3.84e-62 | 209 |
| MsG0780036588.01.T01 | MTR_2g089670 | 31.236 | 461 | 274 | 13 | 24 | 465 | 41 | 477 | 4.42e-58 | 199 |
| MsG0780036588.01.T01 | MTR_3g087640 | 30.689 | 479 | 295 | 15 | 8 | 465 | 10 | 472 | 4.79e-57 | 196 |
| MsG0780036588.01.T01 | MTR_2g089650 | 30.290 | 482 | 292 | 14 | 4 | 465 | 6 | 463 | 6.23e-55 | 190 |
| MsG0780036588.01.T01 | MTR_8g012750 | 30.290 | 482 | 297 | 13 | 7 | 463 | 3 | 470 | 2.85e-54 | 189 |
| MsG0780036588.01.T01 | MTR_2g089755 | 29.978 | 457 | 283 | 11 | 28 | 465 | 37 | 475 | 5.00e-54 | 188 |
| MsG0780036588.01.T01 | MTR_6g015310 | 35.246 | 366 | 177 | 11 | 81 | 443 | 25 | 333 | 7.31e-53 | 181 |
| MsG0780036588.01.T01 | MTR_2g089665 | 27.171 | 449 | 195 | 15 | 7 | 439 | 2 | 334 | 2.90e-34 | 131 |
| MsG0780036588.01.T01 | MTR_7g014310 | 29.882 | 338 | 175 | 9 | 115 | 450 | 31 | 308 | 5.67e-34 | 130 |
| MsG0780036588.01.T01 | MTR_3g435300 | 31.408 | 277 | 168 | 6 | 8 | 272 | 4 | 270 | 1.24e-33 | 128 |
| MsG0780036588.01.T01 | MTR_7g086000 | 26.253 | 499 | 285 | 20 | 6 | 465 | 3 | 457 | 1.95e-23 | 103 |
| MsG0780036588.01.T01 | MTR_6g045553 | 26.411 | 496 | 273 | 21 | 6 | 455 | 13 | 462 | 8.29e-22 | 98.2 |
| MsG0780036588.01.T01 | MTR_5g081960 | 25.630 | 476 | 301 | 19 | 15 | 454 | 14 | 472 | 1.84e-20 | 94.4 |
| MsG0780036588.01.T01 | MTR_8g079950 | 24.888 | 446 | 273 | 15 | 10 | 441 | 8 | 405 | 3.39e-20 | 93.2 |
| MsG0780036588.01.T01 | MTR_3g070170 | 24.894 | 470 | 306 | 17 | 7 | 454 | 12 | 456 | 5.72e-20 | 92.8 |
| MsG0780036588.01.T01 | MTR_4g094838 | 26.535 | 456 | 247 | 17 | 19 | 447 | 19 | 413 | 9.85e-20 | 91.7 |
| MsG0780036588.01.T01 | MTR_8g075610 | 23.502 | 434 | 274 | 18 | 44 | 463 | 30 | 419 | 1.16e-18 | 88.2 |
| MsG0780036588.01.T01 | MTR_3g026950 | 24.065 | 428 | 266 | 16 | 64 | 462 | 51 | 448 | 1.44e-17 | 85.1 |
| MsG0780036588.01.T01 | MTR_8g079970 | 24.664 | 446 | 274 | 18 | 10 | 441 | 8 | 405 | 1.54e-17 | 85.1 |
| MsG0780036588.01.T01 | MTR_8g467580 | 24.138 | 435 | 254 | 16 | 6 | 417 | 4 | 385 | 1.85e-17 | 84.7 |
| MsG0780036588.01.T01 | MTR_2g007880 | 23.733 | 434 | 263 | 16 | 50 | 457 | 48 | 439 | 1.13e-16 | 82.4 |
| MsG0780036588.01.T01 | MTR_8g079980 | 25.676 | 444 | 271 | 17 | 10 | 441 | 8 | 404 | 1.66e-16 | 82.0 |
| MsG0780036588.01.T01 | MTR_5g084850 | 24.605 | 443 | 271 | 18 | 10 | 441 | 8 | 398 | 2.19e-16 | 81.6 |
| MsG0780036588.01.T01 | MTR_4g127650 | 23.146 | 445 | 291 | 15 | 41 | 465 | 43 | 456 | 4.37e-16 | 80.9 |
| MsG0780036588.01.T01 | MTR_3g098980 | 24.757 | 412 | 246 | 17 | 67 | 460 | 66 | 431 | 2.41e-15 | 78.2 |
| MsG0780036588.01.T01 | MTR_4g094842 | 25.728 | 412 | 237 | 18 | 64 | 447 | 59 | 429 | 3.23e-15 | 78.2 |
| MsG0780036588.01.T01 | MTR_4g097090 | 23.093 | 485 | 299 | 19 | 23 | 465 | 4 | 456 | 6.39e-15 | 77.0 |
| MsG0780036588.01.T01 | MTR_1g053315 | 23.218 | 435 | 276 | 21 | 44 | 458 | 58 | 454 | 5.51e-14 | 74.3 |
| MsG0780036588.01.T01 | MTR_3g049150 | 23.237 | 482 | 311 | 20 | 7 | 465 | 2 | 447 | 6.34e-14 | 73.9 |
| MsG0780036588.01.T01 | MTR_3g102010 | 23.043 | 460 | 309 | 18 | 13 | 459 | 15 | 442 | 7.14e-14 | 73.9 |
| MsG0780036588.01.T01 | MTR_3g102020 | 23.110 | 463 | 306 | 20 | 13 | 459 | 14 | 442 | 7.74e-14 | 73.9 |
| MsG0780036588.01.T01 | MTR_7g084940 | 24.094 | 469 | 300 | 16 | 7 | 462 | 16 | 441 | 8.65e-14 | 73.6 |
| MsG0780036588.01.T01 | MTR_4g055170 | 25.066 | 379 | 231 | 17 | 50 | 414 | 59 | 398 | 1.05e-13 | 73.6 |
| MsG0780036588.01.T01 | MTR_3g026310 | 23.333 | 450 | 287 | 14 | 20 | 447 | 16 | 429 | 1.20e-13 | 73.2 |
| MsG0780036588.01.T01 | MTR_8g096530 | 22.343 | 461 | 315 | 15 | 1 | 457 | 1 | 422 | 1.23e-13 | 73.2 |
| MsG0780036588.01.T01 | MTR_7g085990 | 22.567 | 483 | 308 | 22 | 15 | 465 | 9 | 457 | 1.39e-13 | 73.2 |
| MsG0780036588.01.T01 | MTR_8g467590 | 23.776 | 429 | 263 | 15 | 6 | 417 | 4 | 385 | 1.71e-13 | 72.8 |
| MsG0780036588.01.T01 | MTR_8g079710 | 24.105 | 419 | 247 | 19 | 26 | 421 | 27 | 397 | 1.74e-13 | 72.8 |
| MsG0780036588.01.T01 | MTR_3g101960 | 22.246 | 463 | 299 | 18 | 13 | 454 | 14 | 436 | 2.52e-13 | 72.0 |
| MsG0780036588.01.T01 | MTR_2g015430 | 22.149 | 456 | 290 | 19 | 7 | 439 | 2 | 415 | 4.29e-13 | 71.2 |
| MsG0780036588.01.T01 | MTR_8g041770 | 22.940 | 449 | 290 | 15 | 25 | 465 | 27 | 427 | 1.36e-12 | 69.7 |
| MsG0780036588.01.T01 | MTR_4g094832 | 27.139 | 339 | 189 | 16 | 127 | 447 | 113 | 411 | 3.89e-12 | 68.6 |
| MsG0780036588.01.T01 | MTR_4g022825 | 23.750 | 400 | 259 | 12 | 64 | 447 | 52 | 421 | 5.32e-12 | 68.2 |
| MsG0780036588.01.T01 | MTR_7g014205 | 33.913 | 115 | 54 | 3 | 318 | 427 | 17 | 114 | 1.30e-11 | 62.0 |
| MsG0780036588.01.T01 | MTR_3g435290 | 42.857 | 70 | 38 | 1 | 396 | 465 | 50 | 117 | 2.29e-11 | 61.2 |
| MsG0780036588.01.T01 | MTR_0190s0020 | 23.472 | 409 | 243 | 16 | 64 | 447 | 60 | 423 | 3.31e-11 | 65.5 |
| MsG0780036588.01.T01 | MTR_7g014340 | 50.704 | 71 | 29 | 2 | 8 | 75 | 3 | 70 | 3.61e-11 | 60.1 |
| MsG0780036588.01.T01 | MTR_7g014220 | 55.556 | 54 | 21 | 1 | 24 | 77 | 14 | 64 | 4.88e-11 | 59.3 |
| MsG0780036588.01.T01 | MTR_4g022800 | 24.289 | 457 | 272 | 20 | 17 | 447 | 15 | 423 | 5.20e-11 | 65.1 |
| MsG0780036588.01.T01 | MTR_4g022720 | 23.581 | 458 | 267 | 21 | 17 | 447 | 15 | 416 | 5.72e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036588.01.T01 | AT5G39050 | 37.764 | 474 | 268 | 12 | 5 | 465 | 8 | 467 | 4.98e-94 | 292 |
| MsG0780036588.01.T01 | AT5G39080 | 36.498 | 474 | 267 | 13 | 5 | 462 | 3 | 458 | 8.63e-92 | 286 |
| MsG0780036588.01.T01 | AT3G29635 | 37.387 | 444 | 258 | 9 | 26 | 465 | 27 | 454 | 3.86e-91 | 285 |
| MsG0780036588.01.T01 | AT3G29670 | 36.325 | 468 | 270 | 9 | 6 | 465 | 2 | 449 | 6.21e-91 | 284 |
| MsG0780036588.01.T01 | AT5G39090 | 36.659 | 461 | 263 | 12 | 12 | 465 | 9 | 447 | 6.34e-89 | 278 |
| MsG0780036588.01.T01 | AT3G29590 | 37.367 | 471 | 261 | 14 | 3 | 465 | 4 | 448 | 9.30e-89 | 278 |
| MsG0780036588.01.T01 | AT5G61160 | 33.045 | 463 | 287 | 10 | 7 | 463 | 3 | 448 | 2.12e-78 | 251 |
| MsG0780036588.01.T01 | AT3G29680 | 33.790 | 438 | 271 | 7 | 26 | 461 | 23 | 443 | 3.15e-75 | 243 |
| MsG0780036588.01.T01 | AT1G03940 | 31.612 | 484 | 291 | 14 | 1 | 465 | 2 | 464 | 1.02e-65 | 219 |
| MsG0780036588.01.T01 | AT1G03495 | 32.945 | 343 | 198 | 12 | 9 | 337 | 10 | 334 | 2.25e-41 | 151 |
| MsG0780036588.01.T01 | AT5G23940 | 25.806 | 465 | 287 | 22 | 12 | 454 | 10 | 438 | 7.00e-23 | 101 |
| MsG0780036588.01.T01 | AT5G57840 | 24.843 | 479 | 297 | 23 | 7 | 465 | 3 | 438 | 1.49e-18 | 88.2 |
| MsG0780036588.01.T01 | AT3G29690 | 28.758 | 153 | 101 | 3 | 314 | 465 | 33 | 178 | 6.00e-18 | 82.0 |
| MsG0780036588.01.T01 | AT5G48930 | 23.679 | 473 | 302 | 21 | 7 | 463 | 3 | 432 | 1.16e-17 | 85.5 |
| MsG0780036588.01.T01 | AT3G29720 | 30.144 | 209 | 117 | 7 | 104 | 301 | 26 | 216 | 2.99e-17 | 80.9 |
| MsG0780036588.01.T01 | AT1G28680 | 21.690 | 355 | 250 | 7 | 67 | 420 | 62 | 389 | 2.23e-16 | 81.6 |
| MsG0780036588.01.T01 | AT5G41040 | 24.868 | 378 | 205 | 20 | 67 | 417 | 69 | 394 | 3.02e-15 | 78.2 |
| MsG0780036588.01.T01 | AT5G41040 | 24.868 | 378 | 205 | 20 | 67 | 417 | 85 | 410 | 3.54e-15 | 78.2 |
| MsG0780036588.01.T01 | AT1G31490 | 23.251 | 443 | 286 | 18 | 13 | 443 | 14 | 414 | 1.06e-14 | 76.6 |
| MsG0780036588.01.T01 | AT5G42830 | 22.947 | 414 | 271 | 16 | 62 | 459 | 59 | 440 | 2.29e-14 | 75.5 |
| MsG0780036588.01.T01 | AT5G07850 | 23.153 | 406 | 269 | 15 | 28 | 421 | 31 | 405 | 1.17e-13 | 73.2 |
| MsG0780036588.01.T01 | AT3G50280 | 23.206 | 418 | 253 | 15 | 26 | 421 | 25 | 396 | 2.83e-12 | 68.9 |
| MsG0780036588.01.T01 | AT2G25150 | 22.518 | 413 | 264 | 16 | 67 | 457 | 67 | 445 | 1.36e-11 | 67.0 |
| MsG0780036588.01.T01 | AT5G47950 | 22.151 | 465 | 273 | 18 | 14 | 457 | 12 | 408 | 3.60e-11 | 65.5 |
| MsG0780036588.01.T01 | AT5G67160 | 24.578 | 415 | 256 | 13 | 15 | 417 | 13 | 382 | 6.23e-11 | 64.7 |
Find 75 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTATATTGGAAACTGTATT+TGG | 0.115652 | 7:+10156816 | MsG0780036588.01.T01:CDS |
| GAAGGATCTGATTGTGAATT+TGG | 0.161504 | 7:-10155972 | None:intergenic |
| CCAATATAGTTATTTGTTAT+TGG | 0.192492 | 7:-10156803 | None:intergenic |
| GAATTTGTTGATTCCAAATA+TGG | 0.206007 | 7:-10156224 | None:intergenic |
| GAAAGTGAAGATTTAAGATT+TGG | 0.228104 | 7:-10156017 | None:intergenic |
| CAATAGAGTTGGTGTCTCTT+TGG | 0.272806 | 7:-10156396 | None:intergenic |
| CTTTAGCTGGTAACATCGTT+TGG | 0.278608 | 7:+10156063 | MsG0780036588.01.T01:CDS |
| CAAAATTATAAAGAGAAGTT+TGG | 0.293967 | 7:+10156740 | MsG0780036588.01.T01:CDS |
| CGAGACAAAGAAGCTTCTTT+TGG | 0.294661 | 7:-10156191 | None:intergenic |
| CCAATAACAAATAACTATAT+TGG | 0.312347 | 7:+10156803 | MsG0780036588.01.T01:CDS |
| ATGATTTCCCTGTTGAATAA+AGG | 0.315079 | 7:-10156431 | None:intergenic |
| TGAACACAGTCTTTGAGTTT+TGG | 0.321373 | 7:-10156551 | None:intergenic |
| CTATATTGGAAACTGTATTT+GGG | 0.326422 | 7:+10156817 | MsG0780036588.01.T01:CDS |
| TTGATCGAGTTATTTCCATT+TGG | 0.330227 | 7:-10156461 | None:intergenic |
| TGATCGAGTTATTTCCATTT+GGG | 0.330700 | 7:-10156460 | None:intergenic |
| TAACCTCAAACGCGAAGATT+TGG | 0.330845 | 7:+10156583 | MsG0780036588.01.T01:CDS |
| TGAGAGAAATTGAGGTTGTT+TGG | 0.331680 | 7:-10155887 | None:intergenic |
| AAGAAGTATCCAACTCAAAA+AGG | 0.338145 | 7:+10156494 | MsG0780036588.01.T01:CDS |
| AGTTAGAACCTTTATTCAAC+AGG | 0.363008 | 7:+10156423 | MsG0780036588.01.T01:CDS |
| AATAAAGATGATAAATGAAA+AGG | 0.396556 | 7:+10156919 | MsG0780036588.01.T01:CDS |
| TTTAGCTGGTAACATCGTTT+GGG | 0.399158 | 7:+10156064 | MsG0780036588.01.T01:CDS |
| AAGGATCTGATTGTGAATTT+GGG | 0.405438 | 7:-10155971 | None:intergenic |
| AATCATATTCATCAAAGCTT+GGG | 0.406333 | 7:+10156346 | MsG0780036588.01.T01:CDS |
| TTGTGTTTGCTGTAGATTGT+AGG | 0.411131 | 7:+10156765 | MsG0780036588.01.T01:CDS |
| CAATCATATTCATCAAAGCT+TGG | 0.416022 | 7:+10156345 | MsG0780036588.01.T01:CDS |
| GTTGCAGGGTCTAATAGATT+TGG | 0.421014 | 7:+10157019 | MsG0780036588.01.T01:CDS |
| TAGAAGAAGATGTATTCTAT+TGG | 0.422738 | 7:-10155942 | None:intergenic |
| CAACACTTTCTTCCTTTAGC+TGG | 0.427381 | 7:+10156050 | MsG0780036588.01.T01:CDS |
| TATCCAAATCTTCGCGTTTG+AGG | 0.436406 | 7:-10156586 | None:intergenic |
| GGAAGTGGTGGAGTTGAAGT+TGG | 0.449076 | 7:+10157139 | MsG0780036588.01.T01:CDS |
| ATAAAGATGATAAATGAAAA+GGG | 0.449987 | 7:+10156920 | MsG0780036588.01.T01:CDS |
| TAGAGGTTTAGCCATTGGTT+TGG | 0.455999 | 7:+10157102 | MsG0780036588.01.T01:CDS |
| ATTGTTTGAAAGAAGAATGA+AGG | 0.457453 | 7:-10155990 | None:intergenic |
| GACACTATTTCCACCTTGGT+TGG | 0.465401 | 7:-10157073 | None:intergenic |
| CATTCTTCTTTGACAAGAGA+AGG | 0.471649 | 7:+10156980 | MsG0780036588.01.T01:CDS |
| ATATAGTTATTTGTTATTGG+TGG | 0.476303 | 7:-10156800 | None:intergenic |
| GAAGTGGTGGAGTTGAAGTT+GGG | 0.477101 | 7:+10157140 | MsG0780036588.01.T01:CDS |
| GTATGACATCGATCTTGGTT+GGG | 0.485263 | 7:+10157045 | MsG0780036588.01.T01:CDS |
| GGTGTGTATGACATCGATCT+TGG | 0.491228 | 7:+10157040 | MsG0780036588.01.T01:CDS |
| GAAATCATTCAAGACCCAAA+TGG | 0.494774 | 7:+10156446 | MsG0780036588.01.T01:CDS |
| ATAGATAGAGGTTTAGCCAT+TGG | 0.495902 | 7:+10157097 | MsG0780036588.01.T01:CDS |
| GAGGTTGTTTGGCTTGATGA+TGG | 0.497777 | 7:-10155876 | None:intergenic |
| TTTAGCACATTGTTTCATGA+AGG | 0.497997 | 7:+10157193 | MsG0780036588.01.T01:CDS |
| GAAATAGTGTCGATAGATAG+AGG | 0.506319 | 7:+10157085 | MsG0780036588.01.T01:CDS |
| TTTGGATAATATAAAGCAAA+GGG | 0.509625 | 7:+10156601 | MsG0780036588.01.T01:CDS |
| GGTTTGGCAGATAGCAAAGA+TGG | 0.516069 | 7:+10157118 | MsG0780036588.01.T01:CDS |
| CAAACGATGTTACCAGCTAA+AGG | 0.520252 | 7:-10156062 | None:intergenic |
| ATACAATACACATCAAATGA+TGG | 0.528754 | 7:+10156110 | MsG0780036588.01.T01:CDS |
| AGAAGATGTATTCTATTGGA+TGG | 0.532365 | 7:-10155938 | None:intergenic |
| TGTATGACATCGATCTTGGT+TGG | 0.532900 | 7:+10157044 | MsG0780036588.01.T01:CDS |
| CAGCTAAAGGAAGAAAGTGT+TGG | 0.536065 | 7:-10156049 | None:intergenic |
| TATCGACACTATTTCCACCT+TGG | 0.537400 | 7:-10157077 | None:intergenic |
| TCTAACTCTTGCAATAGAGT+TGG | 0.540105 | 7:-10156407 | None:intergenic |
| ATCTGACTCTGCAATGAGCA+AGG | 0.563250 | 7:-10156138 | None:intergenic |
| AGCAAAGGGTGTTATCAACA+TGG | 0.567130 | 7:+10156615 | MsG0780036588.01.T01:CDS |
| ATTTGGATAATATAAAGCAA+AGG | 0.581790 | 7:+10156600 | MsG0780036588.01.T01:CDS |
| TATATTGGAAACTGTATTTG+GGG | 0.583108 | 7:+10156818 | MsG0780036588.01.T01:CDS |
| GCAGATAGCAAAGATGGAAG+TGG | 0.586790 | 7:+10157124 | MsG0780036588.01.T01:CDS |
| TATGACATCGATCTTGGTTG+GGG | 0.590390 | 7:+10157046 | MsG0780036588.01.T01:CDS |
| CTAAAGGAAGAAAGTGTTGG+AGG | 0.593641 | 7:-10156046 | None:intergenic |
| TTTGCTATCTGCCAAACCAA+TGG | 0.594252 | 7:-10157113 | None:intergenic |
| ACTCATCATGCTGTTCTCGA+TGG | 0.594793 | 7:+10156314 | MsG0780036588.01.T01:CDS |
| ATGATGAGTGCTTATTCCAA+TGG | 0.600520 | 7:-10156300 | None:intergenic |
| GCAAAGGGTGTTATCAACAT+GGG | 0.614173 | 7:+10156616 | MsG0780036588.01.T01:CDS |
| GATCTGATTGTGAATTTGGG+AGG | 0.617444 | 7:-10155968 | None:intergenic |
| GATAGCAAAGATGGAAGTGG+TGG | 0.619907 | 7:+10157127 | MsG0780036588.01.T01:CDS |
| TTGCTGTAGATTGTAGGACA+AGG | 0.630737 | 7:+10156771 | MsG0780036588.01.T01:CDS |
| GTTAGAACCTTTATTCAACA+GGG | 0.632568 | 7:+10156424 | MsG0780036588.01.T01:CDS |
| ATCTGATTGTGAATTTGGGA+GGG | 0.632966 | 7:-10155967 | None:intergenic |
| GTTGTTTGGCTTGATGATGG+AGG | 0.643269 | 7:-10155873 | None:intergenic |
| TAAAGGAAGAAAGTGTTGGA+GGG | 0.658167 | 7:-10156045 | None:intergenic |
| CAAGCGAATAAGCACAAGCG+AGG | 0.659456 | 7:-10156688 | None:intergenic |
| ATATTGGAAACTGTATTTGG+GGG | 0.674502 | 7:+10156819 | MsG0780036588.01.T01:CDS |
| TGGTTGGGGAAAACCAACCA+AGG | 0.692930 | 7:+10157060 | MsG0780036588.01.T01:CDS |
| TTGGGGAAAACCAACCAAGG+TGG | 0.693644 | 7:+10157063 | MsG0780036588.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAGATGATAAATGAAA+AGG | + | Chr7:10156919-10156938 | MsG0780036588.01.T01:CDS | 15.0% |
| !! | ATAAAGATGATAAATGAAAA+GGG | + | Chr7:10156920-10156939 | MsG0780036588.01.T01:CDS | 15.0% |
| !!! | TCTTCTTTTATAAAATCTAA+CGG | - | Chr7:10156863-10156882 | None:intergenic | 15.0% |
| !!! | TTAGATTTTATAAAAGAAGA+TGG | + | Chr7:10156863-10156882 | MsG0780036588.01.T01:CDS | 15.0% |
| !! | ATTTGGATAATATAAAGCAA+AGG | + | Chr7:10156600-10156619 | MsG0780036588.01.T01:CDS | 20.0% |
| !! | CAAAATTATAAAGAGAAGTT+TGG | + | Chr7:10156740-10156759 | MsG0780036588.01.T01:CDS | 20.0% |
| !! | CCAATAACAAATAACTATAT+TGG | + | Chr7:10156803-10156822 | MsG0780036588.01.T01:CDS | 20.0% |
| !! | TTTGGATAATATAAAGCAAA+GGG | + | Chr7:10156601-10156620 | MsG0780036588.01.T01:CDS | 20.0% |
| !!! | ATATAGTTATTTGTTATTGG+TGG | - | Chr7:10156803-10156822 | None:intergenic | 20.0% |
| !!! | CCAATATAGTTATTTGTTAT+TGG | - | Chr7:10156806-10156825 | None:intergenic | 20.0% |
| !!! | CTTTATAATTTTGAGCTTTA+TGG | - | Chr7:10156733-10156752 | None:intergenic | 20.0% |
| !!! | TCACTTTTTTTGACATATTT+TGG | + | Chr7:10155907-10155926 | MsG0780036588.01.T01:CDS | 20.0% |
| !!! | TTGATGTGTATTGTATTTTT+GGG | - | Chr7:10156106-10156125 | None:intergenic | 20.0% |
| !!! | TTTGATGTGTATTGTATTTT+TGG | - | Chr7:10156107-10156126 | None:intergenic | 20.0% |
| ! | AAAAAAAGTGAGAGAAATTG+AGG | - | Chr7:10155898-10155917 | None:intergenic | 25.0% |
| ! | AATCATATTCATCAAAGCTT+GGG | + | Chr7:10156346-10156365 | MsG0780036588.01.T01:CDS | 25.0% |
| ! | ACTATATTGGAAACTGTATT+TGG | + | Chr7:10156816-10156835 | MsG0780036588.01.T01:CDS | 25.0% |
| ! | ATACAATACACATCAAATGA+TGG | + | Chr7:10156110-10156129 | MsG0780036588.01.T01:CDS | 25.0% |
| ! | ATTGTTTGAAAGAAGAATGA+AGG | - | Chr7:10155993-10156012 | None:intergenic | 25.0% |
| ! | CTATATTGGAAACTGTATTT+GGG | + | Chr7:10156817-10156836 | MsG0780036588.01.T01:CDS | 25.0% |
| ! | TAGAAGAAGATGTATTCTAT+TGG | - | Chr7:10155945-10155964 | None:intergenic | 25.0% |
| ! | TATATTGGAAACTGTATTTG+GGG | + | Chr7:10156818-10156837 | MsG0780036588.01.T01:CDS | 25.0% |
| !! | GAAAGTGAAGATTTAAGATT+TGG | - | Chr7:10156020-10156039 | None:intergenic | 25.0% |
| !! | TCGTTCTTTTATACCATATT+TGG | + | Chr7:10156211-10156230 | MsG0780036588.01.T01:CDS | 25.0% |
| !!! | AAAAACCACTTTTTGGAAAA+AGG | - | Chr7:10156280-10156299 | None:intergenic | 25.0% |
| !!! | AAAACCACTTTTTGGAAAAA+GGG | - | Chr7:10156279-10156298 | None:intergenic | 25.0% |
| !!! | AGAGAAGGATTTGAAATTTT+TGG | + | Chr7:10156995-10157014 | MsG0780036588.01.T01:CDS | 25.0% |
| !!! | GAATTTGTTGATTCCAAATA+TGG | - | Chr7:10156227-10156246 | None:intergenic | 25.0% |
| !!! | TTTTTGACATATTTTGGCTA+AGG | + | Chr7:10155913-10155932 | MsG0780036588.01.T01:CDS | 25.0% |
| !!! | TTTTTGGAAAAAGGGTTATT+TGG | - | Chr7:10156271-10156290 | None:intergenic | 25.0% |
| AAGGATCTGATTGTGAATTT+GGG | - | Chr7:10155974-10155993 | None:intergenic | 30.0% | |
| AGAAGATGTATTCTATTGGA+TGG | - | Chr7:10155941-10155960 | None:intergenic | 30.0% | |
| AGTTAGAACCTTTATTCAAC+AGG | + | Chr7:10156423-10156442 | MsG0780036588.01.T01:CDS | 30.0% | |
| ATATTGGAAACTGTATTTGG+GGG | + | Chr7:10156819-10156838 | MsG0780036588.01.T01:CDS | 30.0% | |
| ATGATTTCCCTGTTGAATAA+AGG | - | Chr7:10156434-10156453 | None:intergenic | 30.0% | |
| CAATCATATTCATCAAAGCT+TGG | + | Chr7:10156345-10156364 | MsG0780036588.01.T01:CDS | 30.0% | |
| GTTAGAACCTTTATTCAACA+GGG | + | Chr7:10156424-10156443 | MsG0780036588.01.T01:CDS | 30.0% | |
| TTTAGCACATTGTTTCATGA+AGG | + | Chr7:10157193-10157212 | MsG0780036588.01.T01:CDS | 30.0% | |
| ! | ATAACCCTTTTTCCAAAAAG+TGG | + | Chr7:10156272-10156291 | MsG0780036588.01.T01:CDS | 30.0% |
| ! | TGATCGAGTTATTTCCATTT+GGG | - | Chr7:10156463-10156482 | None:intergenic | 30.0% |
| ! | TTGATCGAGTTATTTCCATT+TGG | - | Chr7:10156464-10156483 | None:intergenic | 30.0% |
| !! | AAGAAGTATCCAACTCAAAA+AGG | + | Chr7:10156494-10156513 | MsG0780036588.01.T01:CDS | 30.0% |
| !!! | ATGGTGTTTTTTTAGTTGCT+AGG | + | Chr7:10156882-10156901 | MsG0780036588.01.T01:CDS | 30.0% |
| !!! | CTTTGAGTTTTGGTTCAAAA+GGG | - | Chr7:10156544-10156563 | None:intergenic | 30.0% |
| !!! | TCTTTGAGTTTTGGTTCAAA+AGG | - | Chr7:10156545-10156564 | None:intergenic | 30.0% |
| !!! | TTGAAATTTTTGGAGTTGCA+GGG | + | Chr7:10157005-10157024 | MsG0780036588.01.T01:CDS | 30.0% |
| !!! | TTTGAAATTTTTGGAGTTGC+AGG | + | Chr7:10157004-10157023 | MsG0780036588.01.T01:CDS | 30.0% |
| !!! | TTTTTGAGTTGGATACTTCT+TGG | - | Chr7:10156495-10156514 | None:intergenic | 30.0% |
| ATAGATAGAGGTTTAGCCAT+TGG | + | Chr7:10157097-10157116 | MsG0780036588.01.T01:CDS | 35.0% | |
| ATCTGATTGTGAATTTGGGA+GGG | - | Chr7:10155970-10155989 | None:intergenic | 35.0% | |
| ATGATGAGTGCTTATTCCAA+TGG | - | Chr7:10156303-10156322 | None:intergenic | 35.0% | |
| CATTCTTCTTTGACAAGAGA+AGG | + | Chr7:10156980-10156999 | MsG0780036588.01.T01:CDS | 35.0% | |
| GAAATCATTCAAGACCCAAA+TGG | + | Chr7:10156446-10156465 | MsG0780036588.01.T01:CDS | 35.0% | |
| GAAGGATCTGATTGTGAATT+TGG | - | Chr7:10155975-10155994 | None:intergenic | 35.0% | |
| TCTAACTCTTGCAATAGAGT+TGG | - | Chr7:10156410-10156429 | None:intergenic | 35.0% | |
| TGAGAGAAATTGAGGTTGTT+TGG | - | Chr7:10155890-10155909 | None:intergenic | 35.0% | |
| TTTAGCTGGTAACATCGTTT+GGG | + | Chr7:10156064-10156083 | MsG0780036588.01.T01:CDS | 35.0% | |
| ! | ACGAACGATGATAGATTTTG+TGG | - | Chr7:10156668-10156687 | None:intergenic | 35.0% |
| ! | CCAAAAAGTGGTTTTTCCAT+TGG | + | Chr7:10156284-10156303 | MsG0780036588.01.T01:CDS | 35.0% |
| ! | TGAAAAGGGTTTTCTTGAAG+AGG | + | Chr7:10156934-10156953 | MsG0780036588.01.T01:CDS | 35.0% |
| !! | CCAATGGAAAAACCACTTTT+TGG | - | Chr7:10156287-10156306 | None:intergenic | 35.0% |
| !! | GAAATAGTGTCGATAGATAG+AGG | + | Chr7:10157085-10157104 | MsG0780036588.01.T01:CDS | 35.0% |
| !! | TAAAGGAAGAAAGTGTTGGA+GGG | - | Chr7:10156048-10156067 | None:intergenic | 35.0% |
| !! | TGAACACAGTCTTTGAGTTT+TGG | - | Chr7:10156554-10156573 | None:intergenic | 35.0% |
| !! | TTGTGTTTGCTGTAGATTGT+AGG | + | Chr7:10156765-10156784 | MsG0780036588.01.T01:CDS | 35.0% |
| !!! | CTTTCGTTTCCTTTTTGAGT+TGG | - | Chr7:10156506-10156525 | None:intergenic | 35.0% |
| CAAACGATGTTACCAGCTAA+AGG | - | Chr7:10156065-10156084 | None:intergenic | 40.0% | |
| CAACACTTTCTTCCTTTAGC+TGG | + | Chr7:10156050-10156069 | MsG0780036588.01.T01:CDS | 40.0% | |
| CTTTAGCTGGTAACATCGTT+TGG | + | Chr7:10156063-10156082 | MsG0780036588.01.T01:CDS | 40.0% | |
| GATCTGATTGTGAATTTGGG+AGG | - | Chr7:10155971-10155990 | None:intergenic | 40.0% | |
| GTATGACATCGATCTTGGTT+GGG | + | Chr7:10157045-10157064 | MsG0780036588.01.T01:CDS | 40.0% | |
| GTTGCAGGGTCTAATAGATT+TGG | + | Chr7:10157019-10157038 | MsG0780036588.01.T01:CDS | 40.0% | |
| TAACCTCAAACGCGAAGATT+TGG | + | Chr7:10156583-10156602 | MsG0780036588.01.T01:CDS | 40.0% | |
| TATCCAAATCTTCGCGTTTG+AGG | - | Chr7:10156589-10156608 | None:intergenic | 40.0% | |
| TATCGACACTATTTCCACCT+TGG | - | Chr7:10157080-10157099 | None:intergenic | 40.0% | |
| TATGACATCGATCTTGGTTG+GGG | + | Chr7:10157046-10157065 | MsG0780036588.01.T01:CDS | 40.0% | |
| TGTATGACATCGATCTTGGT+TGG | + | Chr7:10157044-10157063 | MsG0780036588.01.T01:CDS | 40.0% | |
| TTGCTGTAGATTGTAGGACA+AGG | + | Chr7:10156771-10156790 | MsG0780036588.01.T01:CDS | 40.0% | |
| ! | CAGCTAAAGGAAGAAAGTGT+TGG | - | Chr7:10156052-10156071 | None:intergenic | 40.0% |
| ! | CGAGACAAAGAAGCTTCTTT+TGG | - | Chr7:10156194-10156213 | None:intergenic | 40.0% |
| ! | TAGAGGTTTAGCCATTGGTT+TGG | + | Chr7:10157102-10157121 | MsG0780036588.01.T01:CDS | 40.0% |
| ! | TTTGCTATCTGCCAAACCAA+TGG | - | Chr7:10157116-10157135 | None:intergenic | 40.0% |
| !! | AGCAAAGGGTGTTATCAACA+TGG | + | Chr7:10156615-10156634 | MsG0780036588.01.T01:CDS | 40.0% |
| !! | CAATAGAGTTGGTGTCTCTT+TGG | - | Chr7:10156399-10156418 | None:intergenic | 40.0% |
| !! | CTAAAGGAAGAAAGTGTTGG+AGG | - | Chr7:10156049-10156068 | None:intergenic | 40.0% |
| !! | GCAAAGGGTGTTATCAACAT+GGG | + | Chr7:10156616-10156635 | MsG0780036588.01.T01:CDS | 40.0% |
| ACTCATCATGCTGTTCTCGA+TGG | + | Chr7:10156314-10156333 | MsG0780036588.01.T01:CDS | 45.0% | |
| ATCTGACTCTGCAATGAGCA+AGG | - | Chr7:10156141-10156160 | None:intergenic | 45.0% | |
| GACACTATTTCCACCTTGGT+TGG | - | Chr7:10157076-10157095 | None:intergenic | 45.0% | |
| GATAGCAAAGATGGAAGTGG+TGG | + | Chr7:10157127-10157146 | MsG0780036588.01.T01:CDS | 45.0% | |
| GCAGATAGCAAAGATGGAAG+TGG | + | Chr7:10157124-10157143 | MsG0780036588.01.T01:CDS | 45.0% | |
| GGTGTGTATGACATCGATCT+TGG | + | Chr7:10157040-10157059 | MsG0780036588.01.T01:CDS | 45.0% | |
| GGTTTGGCAGATAGCAAAGA+TGG | + | Chr7:10157118-10157137 | MsG0780036588.01.T01:CDS | 45.0% | |
| ! | GAAGTGGTGGAGTTGAAGTT+GGG | + | Chr7:10157140-10157159 | MsG0780036588.01.T01:CDS | 45.0% |
| !! | GAGGTTGTTTGGCTTGATGA+TGG | - | Chr7:10155879-10155898 | None:intergenic | 45.0% |
| !! | GTTGTTTGGCTTGATGATGG+AGG | - | Chr7:10155876-10155895 | None:intergenic | 45.0% |
| CAAGCGAATAAGCACAAGCG+AGG | - | Chr7:10156691-10156710 | None:intergenic | 50.0% | |
| TGGTTGGGGAAAACCAACCA+AGG | + | Chr7:10157060-10157079 | MsG0780036588.01.T01:CDS | 50.0% | |
| TTGGGGAAAACCAACCAAGG+TGG | + | Chr7:10157063-10157082 | MsG0780036588.01.T01:CDS | 50.0% | |
| ! | GGAAGTGGTGGAGTTGAAGT+TGG | + | Chr7:10157139-10157158 | MsG0780036588.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 10155825 | 10157231 | 10155825 | ID=MsG0780036588.01;Name=MsG0780036588.01 |
| Chr7 | mRNA | 10155825 | 10157231 | 10155825 | ID=MsG0780036588.01.T01;Parent=MsG0780036588.01;Name=MsG0780036588.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|468 |
| Chr7 | exon | 10155825 | 10157231 | 10155825 | ID=MsG0780036588.01.T01:exon:10402;Parent=MsG0780036588.01.T01 |
| Chr7 | CDS | 10155825 | 10157231 | 10155825 | ID=MsG0780036588.01.T01:cds;Parent=MsG0780036588.01.T01 |
| Gene Sequence |
| Protein sequence |
