Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036582.01.T01 | AAB88294.1 | 98.251 | 343 | 6 | 0 | 1 | 343 | 1 | 343 | 0 | 694 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036582.01.T01 | O22308 | 95.739 | 352 | 6 | 1 | 1 | 343 | 1 | 352 | 0 | 686 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036582.01.T01 | O22555 | 98.251 | 343 | 6 | 0 | 1 | 343 | 1 | 343 | 0.0 | 694 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048177.01 | MsG0780036582.01 | 0.804789 | 1.824710e-49 | 9.494357e-47 |
| MsG0180000197.01 | MsG0780036582.01 | 0.817244 | 3.647248e-52 | 2.641936e-49 |
| MsG0180001922.01 | MsG0780036582.01 | 0.822742 | 2.013497e-53 | 1.700012e-50 |
| MsG0180002912.01 | MsG0780036582.01 | 0.811115 | 8.225677e-51 | 5.048559e-48 |
| MsG0180003241.01 | MsG0780036582.01 | 0.817131 | 3.866835e-52 | 2.792405e-49 |
| MsG0180004116.01 | MsG0780036582.01 | 0.805783 | 1.129822e-49 | 6.030826e-47 |
| MsG0180005125.01 | MsG0780036582.01 | 0.801350 | 9.387124e-49 | 4.473010e-46 |
| MsG0180005949.01 | MsG0780036582.01 | 0.822776 | 1.976152e-53 | 1.670099e-50 |
| MsG0380011754.01 | MsG0780036582.01 | 0.806348 | 8.591427e-50 | 4.652445e-47 |
| MsG0380012458.01 | MsG0780036582.01 | 0.816731 | 4.753287e-52 | 3.395182e-49 |
| MsG0380014913.01 | MsG0780036582.01 | 0.808882 | 2.488852e-50 | 1.439677e-47 |
| MsG0680030841.01 | MsG0780036582.01 | 0.805507 | 1.291229e-49 | 6.843117e-47 |
| MsG0680031694.01 | MsG0780036582.01 | 0.805097 | 1.573799e-49 | 8.253518e-47 |
| MsG0680034672.01 | MsG0780036582.01 | 0.827123 | 1.861321e-54 | 1.780405e-51 |
| MsG0680035048.01 | MsG0780036582.01 | 0.804850 | 1.772084e-49 | 9.235199e-47 |
| MsG0480020242.01 | MsG0780036582.01 | 0.805437 | 1.335749e-49 | 7.066574e-47 |
| MsG0480023517.01 | MsG0780036582.01 | 0.804798 | 1.817254e-49 | 9.457603e-47 |
| MsG0580025278.01 | MsG0780036582.01 | 0.812986 | 3.215784e-51 | 2.074407e-48 |
| MsG0580025520.01 | MsG0780036582.01 | 0.806536 | 7.844272e-50 | 4.268315e-47 |
| MsG0580025769.01 | MsG0780036582.01 | 0.825420 | 4.732576e-54 | 4.311384e-51 |
| MsG0380016227.01 | MsG0780036582.01 | 0.808287 | 3.335903e-50 | 1.899875e-47 |
| MsG0380017158.01 | MsG0780036582.01 | 0.821433 | 4.049229e-53 | 3.293785e-50 |
| MsG0380017226.01 | MsG0780036582.01 | 0.806020 | 1.007499e-49 | 5.410618e-47 |
| MsG0580027129.01 | MsG0780036582.01 | 0.810757 | 9.833871e-51 | 5.978691e-48 |
| MsG0580029658.01 | MsG0780036582.01 | 0.820147 | 7.997596e-53 | 6.277738e-50 |
| MsG0280006728.01 | MsG0780036582.01 | 0.806397 | 8.389506e-50 | 4.548709e-47 |
| MsG0280006935.01 | MsG0780036582.01 | 0.812754 | 3.615817e-51 | 2.318161e-48 |
| MsG0280007258.01 | MsG0780036582.01 | 0.829360 | 5.377792e-55 | 5.492018e-52 |
| MsG0280007439.01 | MsG0780036582.01 | 0.800963 | 1.126073e-48 | 5.313947e-46 |
| MsG0280008220.01 | MsG0780036582.01 | 0.811587 | 6.498988e-51 | 4.038617e-48 |
| MsG0280008295.01 | MsG0780036582.01 | 0.806216 | 9.161931e-50 | 4.945000e-47 |
| MsG0780036582.01 | MsG0780038444.01 | 0.827488 | 1.521494e-54 | 1.471020e-51 |
| MsG0780036582.01 | MsG0780040389.01 | 0.811496 | 6.801349e-51 | 4.216656e-48 |
| MsG0780036582.01 | MsG0780040516.01 | 0.806302 | 8.787911e-50 | 4.753268e-47 |
| MsG0780036582.01 | MsG0780040679.01 | 0.809367 | 1.959395e-50 | 1.148294e-47 |
| MsG0780036582.01 | MsG0780040929.01 | 0.815838 | 7.530287e-52 | 5.248768e-49 |
| MsG0780036582.01 | MsG0780041376.01 | 0.801844 | 7.431523e-49 | 3.585786e-46 |
| MsG0780036582.01 | MsG0880043001.01 | 0.800007 | 1.763632e-48 | 8.122505e-46 |
| MsG0780036582.01 | MsG0880043288.01 | 0.802572 | 5.264847e-49 | 2.587710e-46 |
| MsG0780036582.01 | MsG0880043489.01 | 0.815696 | 8.098711e-52 | 5.623373e-49 |
| MsG0780036582.01 | MsG0880044039.01 | 0.807829 | 4.175402e-50 | 2.349371e-47 |
| MsG0780036582.01 | MsG0880044546.01 | 0.821297 | 4.351549e-53 | 3.526315e-50 |
| MsG0780036582.01 | MsG0880045914.01 | 0.822137 | 2.782251e-53 | 2.308824e-50 |
| MsG0780036582.01 | MsG0880046047.01 | 0.801241 | 9.878826e-49 | 4.694534e-46 |
| MsG0780036582.01 | MsG0880046684.01 | 0.803056 | 4.181793e-49 | 2.080852e-46 |
| MsG0780036582.01 | MsG0880046731.01 | 0.836645 | 8.286771e-57 | 1.050711e-53 |
| MsG0780036582.01 | MsG0880046888.01 | 0.812948 | 3.278602e-51 | 2.112724e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036582.01.T01 | MTR_7g014570 | 94.602 | 352 | 10 | 1 | 1 | 343 | 1 | 352 | 0.0 | 679 |
| MsG0780036582.01.T01 | MTR_7g014510 | 78.992 | 357 | 61 | 2 | 1 | 343 | 1 | 357 | 0.0 | 595 |
| MsG0780036582.01.T01 | MTR_1g076940 | 57.064 | 361 | 137 | 4 | 1 | 343 | 1 | 361 | 6.82e-144 | 411 |
| MsG0780036582.01.T01 | MTR_4g088345 | 56.461 | 356 | 142 | 4 | 1 | 343 | 1 | 356 | 4.07e-136 | 391 |
| MsG0780036582.01.T01 | MTR_7g024500 | 54.286 | 350 | 144 | 4 | 4 | 341 | 3 | 348 | 3.93e-130 | 376 |
| MsG0780036582.01.T01 | MTR_4g088340 | 55.556 | 333 | 135 | 4 | 24 | 343 | 1 | 333 | 2.28e-125 | 363 |
| MsG0780036582.01.T01 | MTR_7g024520 | 51.714 | 350 | 153 | 4 | 4 | 341 | 3 | 348 | 1.58e-124 | 362 |
| MsG0780036582.01.T01 | MTR_4g033015 | 50.964 | 363 | 158 | 5 | 1 | 343 | 1 | 363 | 3.15e-116 | 341 |
| MsG0780036582.01.T01 | MTR_4g033200 | 50.964 | 363 | 158 | 5 | 1 | 343 | 1 | 363 | 1.61e-115 | 339 |
| MsG0780036582.01.T01 | MTR_4g088200 | 47.091 | 361 | 173 | 5 | 1 | 343 | 1 | 361 | 1.43e-111 | 329 |
| MsG0780036582.01.T01 | MTR_4g033150 | 49.587 | 363 | 163 | 5 | 1 | 343 | 1 | 363 | 4.98e-110 | 325 |
| MsG0780036582.01.T01 | MTR_4g079160 | 45.376 | 346 | 176 | 5 | 11 | 343 | 10 | 355 | 8.04e-108 | 319 |
| MsG0780036582.01.T01 | MTR_4g088190 | 48.626 | 364 | 166 | 6 | 1 | 343 | 1 | 364 | 4.62e-107 | 318 |
| MsG0780036582.01.T01 | MTR_5g074600 | 46.132 | 349 | 172 | 6 | 11 | 343 | 11 | 359 | 9.09e-107 | 317 |
| MsG0780036582.01.T01 | MTR_5g074580 | 46.132 | 349 | 172 | 5 | 11 | 343 | 12 | 360 | 9.95e-106 | 314 |
| MsG0780036582.01.T01 | MTR_5g074680 | 45.977 | 348 | 172 | 6 | 11 | 342 | 12 | 359 | 4.36e-105 | 312 |
| MsG0780036582.01.T01 | MTR_4g033170 | 49.716 | 352 | 163 | 5 | 6 | 343 | 6 | 357 | 1.40e-103 | 308 |
| MsG0780036582.01.T01 | MTR_4g033085 | 48.864 | 352 | 166 | 5 | 6 | 343 | 6 | 357 | 2.57e-97 | 292 |
| MsG0780036582.01.T01 | MTR_5g074540 | 41.408 | 355 | 182 | 5 | 1 | 343 | 1 | 341 | 2.69e-94 | 284 |
| MsG0780036582.01.T01 | MTR_5g074610 | 43.228 | 347 | 156 | 5 | 11 | 343 | 7 | 326 | 2.97e-93 | 281 |
| MsG0780036582.01.T01 | MTR_1g076930 | 54.008 | 237 | 102 | 3 | 24 | 253 | 1 | 237 | 2.58e-81 | 248 |
| MsG0780036582.01.T01 | MTR_0794s0020 | 48.996 | 249 | 117 | 3 | 1 | 239 | 1 | 249 | 2.40e-76 | 235 |
| MsG0780036582.01.T01 | MTR_3g083620 | 33.631 | 336 | 202 | 8 | 20 | 339 | 27 | 357 | 1.89e-54 | 182 |
| MsG0780036582.01.T01 | MTR_0017s0100 | 32.086 | 374 | 217 | 10 | 1 | 343 | 1 | 368 | 1.69e-51 | 175 |
| MsG0780036582.01.T01 | MTR_4g038440 | 32.584 | 356 | 214 | 9 | 8 | 343 | 19 | 368 | 5.76e-50 | 171 |
| MsG0780036582.01.T01 | MTR_0079s0070 | 33.531 | 337 | 198 | 9 | 27 | 343 | 4 | 334 | 1.29e-48 | 166 |
| MsG0780036582.01.T01 | MTR_3g092900 | 29.155 | 343 | 216 | 7 | 10 | 329 | 11 | 349 | 7.36e-36 | 134 |
| MsG0780036582.01.T01 | MTR_1g036460 | 30.357 | 336 | 204 | 9 | 33 | 342 | 38 | 369 | 1.08e-35 | 133 |
| MsG0780036582.01.T01 | MTR_8g024160 | 30.325 | 277 | 166 | 7 | 82 | 335 | 49 | 321 | 9.83e-35 | 130 |
| MsG0780036582.01.T01 | MTR_7g011900 | 28.527 | 319 | 204 | 7 | 33 | 330 | 41 | 356 | 7.52e-34 | 128 |
| MsG0780036582.01.T01 | MTR_1g036490 | 28.190 | 337 | 210 | 8 | 33 | 342 | 38 | 369 | 2.28e-33 | 127 |
| MsG0780036582.01.T01 | MTR_7g012040 | 27.760 | 317 | 206 | 7 | 33 | 329 | 40 | 353 | 4.51e-33 | 126 |
| MsG0780036582.01.T01 | MTR_7g118300 | 28.931 | 318 | 202 | 7 | 31 | 328 | 47 | 360 | 9.80e-33 | 125 |
| MsG0780036582.01.T01 | MTR_7g012070 | 27.160 | 324 | 205 | 8 | 33 | 329 | 41 | 360 | 4.93e-32 | 123 |
| MsG0780036582.01.T01 | MTR_5g098170 | 30.581 | 327 | 191 | 9 | 31 | 330 | 33 | 350 | 5.65e-32 | 123 |
| MsG0780036582.01.T01 | MTR_7g011990 | 26.807 | 332 | 215 | 8 | 33 | 342 | 41 | 366 | 9.08e-32 | 122 |
| MsG0780036582.01.T01 | MTR_1g096310 | 28.528 | 326 | 197 | 9 | 33 | 330 | 41 | 358 | 9.58e-32 | 122 |
| MsG0780036582.01.T01 | MTR_4g038440 | 31.373 | 255 | 163 | 5 | 8 | 253 | 19 | 270 | 1.29e-31 | 120 |
| MsG0780036582.01.T01 | MTR_8g068690 | 27.673 | 318 | 203 | 7 | 31 | 325 | 45 | 358 | 2.92e-31 | 121 |
| MsG0780036582.01.T01 | MTR_7g012090 | 26.923 | 338 | 215 | 9 | 33 | 342 | 41 | 374 | 3.06e-31 | 121 |
| MsG0780036582.01.T01 | MTR_8g068750 | 26.498 | 317 | 206 | 7 | 31 | 324 | 46 | 358 | 3.09e-31 | 121 |
| MsG0780036582.01.T01 | MTR_7g012020 | 26.506 | 332 | 219 | 8 | 33 | 342 | 40 | 368 | 5.35e-30 | 118 |
| MsG0780036582.01.T01 | MTR_1g036510 | 28.676 | 272 | 163 | 5 | 69 | 330 | 119 | 369 | 5.45e-30 | 118 |
| MsG0780036582.01.T01 | MTR_8g068870 | 27.134 | 328 | 212 | 7 | 24 | 328 | 25 | 348 | 7.36e-30 | 117 |
| MsG0780036582.01.T01 | MTR_0591s0010 | 26.332 | 319 | 204 | 8 | 31 | 324 | 46 | 358 | 8.81e-30 | 117 |
| MsG0780036582.01.T01 | MTR_7g118320 | 26.101 | 318 | 208 | 8 | 30 | 324 | 30 | 343 | 7.47e-29 | 114 |
| MsG0780036582.01.T01 | MTR_3g096050 | 27.324 | 355 | 230 | 12 | 9 | 342 | 10 | 357 | 2.44e-28 | 113 |
| MsG0780036582.01.T01 | MTR_2g072790 | 29.747 | 316 | 201 | 7 | 31 | 328 | 27 | 339 | 4.55e-28 | 112 |
| MsG0780036582.01.T01 | MTR_2g055940 | 31.064 | 235 | 142 | 6 | 122 | 342 | 134 | 362 | 9.47e-28 | 111 |
| MsG0780036582.01.T01 | MTR_6g078850 | 28.514 | 249 | 158 | 7 | 94 | 328 | 126 | 368 | 1.02e-27 | 113 |
| MsG0780036582.01.T01 | MTR_3g021440 | 26.582 | 316 | 210 | 6 | 33 | 329 | 43 | 355 | 2.93e-27 | 110 |
| MsG0780036582.01.T01 | MTR_3g021430 | 26.562 | 320 | 205 | 9 | 33 | 329 | 30 | 342 | 7.18e-27 | 109 |
| MsG0780036582.01.T01 | MTR_3g096070 | 28.267 | 329 | 189 | 12 | 32 | 340 | 31 | 332 | 6.44e-24 | 100 |
| MsG0780036582.01.T01 | MTR_7g012020 | 28.270 | 237 | 159 | 4 | 33 | 258 | 40 | 276 | 8.14e-24 | 99.8 |
| MsG0780036582.01.T01 | MTR_8g068730 | 25.868 | 317 | 176 | 8 | 31 | 324 | 46 | 326 | 4.91e-21 | 92.8 |
| MsG0780036582.01.T01 | MTR_8g068690 | 26.971 | 241 | 163 | 4 | 31 | 259 | 45 | 284 | 7.70e-21 | 91.3 |
| MsG0780036582.01.T01 | MTR_8g068570 | 31.288 | 163 | 98 | 3 | 159 | 310 | 158 | 317 | 2.16e-20 | 90.5 |
| MsG0780036582.01.T01 | MTR_8g068600 | 25.877 | 228 | 156 | 4 | 38 | 253 | 27 | 253 | 4.58e-20 | 89.0 |
| MsG0780036582.01.T01 | MTR_1g036510 | 29.189 | 185 | 113 | 2 | 69 | 253 | 119 | 285 | 4.14e-18 | 84.0 |
| MsG0780036582.01.T01 | MTR_8g068620 | 31.897 | 116 | 76 | 1 | 147 | 262 | 4 | 116 | 3.09e-17 | 77.0 |
| MsG0780036582.01.T01 | MTR_1g050220 | 31.915 | 141 | 91 | 3 | 188 | 327 | 151 | 287 | 3.74e-17 | 81.3 |
| MsG0780036582.01.T01 | MTR_7g012020 | 27.317 | 205 | 138 | 4 | 33 | 226 | 40 | 244 | 7.35e-16 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036582.01.T01 | AT4G35150 | 32.286 | 350 | 175 | 11 | 15 | 342 | 14 | 323 | 5.51e-46 | 159 |
| MsG0780036582.01.T01 | AT4G35160 | 32.961 | 358 | 205 | 11 | 15 | 342 | 28 | 380 | 1.61e-43 | 154 |
| MsG0780036582.01.T01 | AT5G54160 | 28.531 | 354 | 226 | 9 | 10 | 341 | 11 | 359 | 3.32e-37 | 137 |
| MsG0780036582.01.T01 | AT3G53140 | 28.829 | 333 | 211 | 9 | 31 | 340 | 27 | 356 | 4.06e-29 | 115 |
| MsG0780036582.01.T01 | AT1G33030 | 28.274 | 336 | 210 | 13 | 31 | 342 | 21 | 349 | 6.92e-28 | 112 |
| MsG0780036582.01.T01 | AT1G51990 | 27.397 | 292 | 190 | 7 | 71 | 343 | 74 | 362 | 1.33e-27 | 111 |
| MsG0780036582.01.T01 | AT1G51990 | 27.740 | 292 | 189 | 7 | 71 | 343 | 74 | 362 | 4.02e-27 | 110 |
| MsG0780036582.01.T01 | AT1G77520 | 29.130 | 230 | 143 | 5 | 130 | 342 | 154 | 380 | 2.59e-23 | 99.8 |
| MsG0780036582.01.T01 | AT5G37170 | 30.500 | 200 | 117 | 7 | 159 | 342 | 40 | 233 | 7.15e-20 | 87.4 |
| MsG0780036582.01.T01 | AT5G37170 | 30.500 | 200 | 117 | 7 | 159 | 342 | 140 | 333 | 1.76e-19 | 88.2 |
| MsG0780036582.01.T01 | AT5G53810 | 26.801 | 347 | 210 | 10 | 31 | 342 | 40 | 377 | 4.42e-19 | 87.8 |
| MsG0780036582.01.T01 | AT5G37170 | 31.609 | 174 | 100 | 6 | 185 | 342 | 15 | 185 | 2.47e-18 | 82.0 |
| MsG0780036582.01.T01 | AT1G63140 | 29.130 | 230 | 143 | 5 | 130 | 342 | 154 | 380 | 2.04e-17 | 82.8 |
| MsG0780036582.01.T01 | AT1G63140 | 29.130 | 230 | 143 | 5 | 130 | 342 | 174 | 400 | 2.17e-17 | 82.8 |
| MsG0780036582.01.T01 | AT1G33030 | 28.511 | 235 | 153 | 8 | 31 | 253 | 21 | 252 | 6.31e-17 | 79.7 |
| MsG0780036582.01.T01 | AT1G33030 | 28.452 | 239 | 155 | 9 | 31 | 256 | 21 | 256 | 9.10e-17 | 79.7 |
| MsG0780036582.01.T01 | AT1G77530 | 28.696 | 230 | 144 | 5 | 130 | 342 | 154 | 380 | 3.49e-16 | 79.3 |
| MsG0780036582.01.T01 | AT1G62900 | 28.922 | 204 | 130 | 4 | 151 | 342 | 4 | 204 | 5.96e-15 | 73.2 |
| MsG0780036582.01.T01 | AT1G76790 | 27.074 | 229 | 144 | 6 | 111 | 324 | 126 | 346 | 3.35e-12 | 67.4 |
| MsG0780036582.01.T01 | AT1G21100 | 23.028 | 317 | 209 | 10 | 29 | 329 | 62 | 359 | 1.61e-11 | 65.1 |
| MsG0780036582.01.T01 | AT1G21130 | 29.167 | 144 | 87 | 4 | 198 | 329 | 219 | 359 | 3.06e-11 | 64.3 |
Find 57 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGATTCCATGTCTCTTAAA+TGG | 0.124329 | 7:-10086146 | MsG0780036582.01.T01:CDS |
| ACTAAGTTTGAGAGAGAAAT+TGG | 0.197834 | 7:+10086078 | None:intergenic |
| GTAAACATGGCTTGTCTTAA+TGG | 0.208098 | 7:-10084570 | MsG0780036582.01.T01:CDS |
| GTTCTTGATCCAACTCTTTC+GGG | 0.231957 | 7:-10085877 | MsG0780036582.01.T01:CDS |
| ACACTCTTTGGTGTTACTTT+AGG | 0.251861 | 7:-10085811 | MsG0780036582.01.T01:CDS |
| TGTTCTTGATCCAACTCTTT+CGG | 0.264765 | 7:-10085878 | MsG0780036582.01.T01:CDS |
| AAATTGGTTTGCCATGGTTT+TGG | 0.271392 | 7:+10086094 | None:intergenic |
| TACTCAAATTAAGCTCCTTA+TGG | 0.295596 | 7:-10084595 | MsG0780036582.01.T01:CDS |
| TTAAATGATGTATTATATTC+AGG | 0.298723 | 7:+10085757 | None:intergenic |
| GAGGAAGATCTTACACTCTT+TGG | 0.320824 | 7:-10085823 | MsG0780036582.01.T01:CDS |
| GCTTCAGAGCTTCTTGTTAG+AGG | 0.330458 | 7:-10085934 | MsG0780036582.01.T01:CDS |
| CAAGTTCCATCGTCGAAAAT+AGG | 0.334139 | 7:-10086045 | MsG0780036582.01.T01:CDS |
| AAAATGGCATCATCAATTAA+TGG | 0.366895 | 7:-10086231 | MsG0780036582.01.T01:CDS |
| TTTGGTGTTACTTTAGGATC+TGG | 0.377403 | 7:-10085805 | MsG0780036582.01.T01:CDS |
| AAAGAAGCTGTTACAAATGA+TGG | 0.378738 | 7:-10084681 | MsG0780036582.01.T01:CDS |
| CAAGTTGTAGAGAACTTATC+TGG | 0.380400 | 7:-10085565 | MsG0780036582.01.T01:CDS |
| TGAAATGTATTGTGTTTGAT+AGG | 0.411209 | 7:-10085591 | MsG0780036582.01.T01:CDS |
| GTGGATGTTGGTGGTGGAAC+TGG | 0.416127 | 7:-10085652 | MsG0780036582.01.T01:CDS |
| AAAAGTGATTATTATAGATA+TGG | 0.416932 | 7:-10084649 | MsG0780036582.01.T01:CDS |
| AAGAGAGAAATGAGGAAGAA+TGG | 0.419676 | 7:-10084545 | MsG0780036582.01.T01:CDS |
| AGCAAAACTGCATCAGCATT+AGG | 0.422726 | 7:+10085496 | None:intergenic |
| AAGGAGATATCTTATAGTCT+TGG | 0.440862 | 7:+10084496 | None:intergenic |
| TGATTCTAAATTGATAAACT+TGG | 0.458151 | 7:-10085719 | MsG0780036582.01.T01:CDS |
| CGTATCATGAGCTGAAGAAA+TGG | 0.458512 | 7:-10085852 | MsG0780036582.01.T01:CDS |
| AGCAATAATTTGACTTATGT+TGG | 0.474211 | 7:-10085541 | MsG0780036582.01.T01:CDS |
| TATAAGATATCTCCTTTGAC+TGG | 0.475443 | 7:-10084489 | MsG0780036582.01.T01:CDS |
| AGATTCCATGTCTCTTAAAT+GGG | 0.476440 | 7:-10086145 | MsG0780036582.01.T01:CDS |
| TGATCTTTGTTTAGCACCAA+TGG | 0.482495 | 7:-10085908 | MsG0780036582.01.T01:CDS |
| GCTGAAGAAATGGATTTATG+AGG | 0.492483 | 7:-10085842 | MsG0780036582.01.T01:CDS |
| AAGAAACTCTTCATAGAAGC+TGG | 0.494387 | 7:-10084522 | MsG0780036582.01.T01:CDS |
| AAACACAATACATTTCAACT+TGG | 0.506032 | 7:+10085597 | None:intergenic |
| GTTACAAATGATGGGAAAAG+AGG | 0.507671 | 7:-10084672 | MsG0780036582.01.T01:CDS |
| AAGCCATGTTTACGTCCATA+AGG | 0.511052 | 7:+10084580 | None:intergenic |
| ATAATTTGACTTATGTTGGT+GGG | 0.511853 | 7:-10085537 | MsG0780036582.01.T01:CDS |
| AACACAATACATTTCAACTT+GGG | 0.515695 | 7:+10085598 | None:intergenic |
| AACAGCCCATTTAAGAGACA+TGG | 0.517213 | 7:+10086140 | None:intergenic |
| TGATGGATTGGAATCAATTG+TGG | 0.517617 | 7:-10085671 | MsG0780036582.01.T01:CDS |
| TTGGAATCAATTGTGGATGT+TGG | 0.527463 | 7:-10085664 | MsG0780036582.01.T01:CDS |
| AGAGAGAAATTGGTTTGCCA+TGG | 0.531316 | 7:+10086088 | None:intergenic |
| TAATTTGACTTATGTTGGTG+GGG | 0.537579 | 7:-10085536 | MsG0780036582.01.T01:CDS |
| TACATCATTTAATGATGCAA+TGG | 0.539653 | 7:-10085746 | MsG0780036582.01.T01:CDS |
| GCTCCTTATGGACGTAAACA+TGG | 0.540942 | 7:-10084583 | MsG0780036582.01.T01:CDS |
| AAGAGACATGGAATCTATGA+AGG | 0.543388 | 7:+10086152 | None:intergenic |
| CGAAGAATCCGTTGTGCGCG+AGG | 0.543967 | 7:+10085998 | None:intergenic |
| AATAATTTGACTTATGTTGG+TGG | 0.546090 | 7:-10085538 | MsG0780036582.01.T01:CDS |
| GGACCGATAAGGATTGCATA+AGG | 0.547963 | 7:-10084719 | MsG0780036582.01.T01:intron |
| AAGAAGCTGTTACAAATGAT+GGG | 0.548845 | 7:-10084680 | MsG0780036582.01.T01:CDS |
| GAATCAATTGTGGATGTTGG+TGG | 0.561061 | 7:-10085661 | MsG0780036582.01.T01:CDS |
| TGATACGAACCCGAAAGAGT+TGG | 0.566568 | 7:+10085868 | None:intergenic |
| TCAAGAACACACTCAACCAT+TGG | 0.570476 | 7:+10085892 | None:intergenic |
| TAATGGAAAAGAGAGAAATG+AGG | 0.576668 | 7:-10084553 | MsG0780036582.01.T01:CDS |
| TCAATTGTGGATGTTGGTGG+TGG | 0.576859 | 7:-10085658 | MsG0780036582.01.T01:CDS |
| GATAAGTTCTCTACAACTTG+TGG | 0.586582 | 7:+10085568 | None:intergenic |
| TGTGCTTTGAAAATTTCACT+TGG | 0.587882 | 7:+10086201 | None:intergenic |
| CCAAACATAATCCAAAACCA+TGG | 0.655283 | 7:-10086105 | MsG0780036582.01.T01:CDS |
| ATGCGTTACCTCGCGCACAA+CGG | 0.655418 | 7:-10086006 | MsG0780036582.01.T01:CDS |
| CGTCGAAAATAGGTAACGTG+CGG | 0.696937 | 7:-10086035 | MsG0780036582.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATGTAAAATATACTTT+AGG | + | Chr7:10085984-10086003 | None:intergenic | 10.0% |
| !! | ATAAGAAATTACAAAAAATT+TGG | - | Chr7:10085462-10085481 | MsG0780036582.01.T01:intron | 10.0% |
| !! | TAAGAAATTACAAAAAATTT+GGG | - | Chr7:10085463-10085482 | MsG0780036582.01.T01:intron | 10.0% |
| !!! | TAAAGTATATTTTACATAAT+TGG | - | Chr7:10085983-10086002 | MsG0780036582.01.T01:CDS | 10.0% |
| !!! | TTATTTTTTATCTAATCTAA+TGG | - | Chr7:10085702-10085721 | MsG0780036582.01.T01:CDS | 10.0% |
| !! | AAAAGTGATTATTATAGATA+TGG | - | Chr7:10086074-10086093 | MsG0780036582.01.T01:CDS | 15.0% |
| !! | AAATTCATTTGCAAAAAAAA+TGG | - | Chr7:10084476-10084495 | MsG0780036582.01.T01:CDS | 15.0% |
| !! | AATAATTTAAGAAAACTAGA+TGG | + | Chr7:10085584-10085603 | None:intergenic | 15.0% |
| !! | ACAAAAAAAAAAAAAAACAG+AGG | + | Chr7:10085949-10085968 | None:intergenic | 15.0% |
| !! | ATTTATATAAGAAATTTAGG+GGG | - | Chr7:10085433-10085452 | MsG0780036582.01.T01:intron | 15.0% |
| !! | GATTTATATAAGAAATTTAG+GGG | - | Chr7:10085432-10085451 | MsG0780036582.01.T01:intron | 15.0% |
| !! | GGATTTATATAAGAAATTTA+GGG | - | Chr7:10085431-10085450 | MsG0780036582.01.T01:intron | 15.0% |
| !! | TATATAAATCCCTATAAATT+AGG | + | Chr7:10085422-10085441 | None:intergenic | 15.0% |
| !! | TTAAATGATGTATTATATTC+AGG | + | Chr7:10084969-10084988 | None:intergenic | 15.0% |
| !!! | TTTTTTTGCAAATGAATTTT+TGG | + | Chr7:10084475-10084494 | None:intergenic | 15.0% |
| !! | AAAGAAATTGCCTAATTTAT+AGG | - | Chr7:10085409-10085428 | MsG0780036582.01.T01:intron | 20.0% |
| !! | AAGAAATTGCCTAATTTATA+GGG | - | Chr7:10085410-10085429 | MsG0780036582.01.T01:intron | 20.0% |
| !! | ATAAATTGCAACACATTTAT+AGG | - | Chr7:10085347-10085366 | MsG0780036582.01.T01:intron | 20.0% |
| !! | ATTCATTAAGAAATGAGTTA+AGG | + | Chr7:10085902-10085921 | None:intergenic | 20.0% |
| !! | GGGATTTATATAAGAAATTT+AGG | - | Chr7:10085430-10085449 | MsG0780036582.01.T01:intron | 20.0% |
| !! | TAAATTGCAACACATTTATA+GGG | - | Chr7:10085348-10085367 | MsG0780036582.01.T01:intron | 20.0% |
| !! | TGATTCTAAATTGATAAACT+TGG | - | Chr7:10085004-10085023 | MsG0780036582.01.T01:intron | 20.0% |
| !!! | GAAAATTTACTTTGTTTCTT+CGG | - | Chr7:10085850-10085869 | MsG0780036582.01.T01:CDS | 20.0% |
| ! | AAAATGGCATCATCAATTAA+TGG | - | Chr7:10084492-10084511 | MsG0780036582.01.T01:CDS | 25.0% |
| ! | AAACACAATACATTTCAACT+TGG | + | Chr7:10085129-10085148 | None:intergenic | 25.0% |
| ! | AAATTGCAACACATTTATAG+GGG | - | Chr7:10085349-10085368 | MsG0780036582.01.T01:intron | 25.0% |
| ! | AACACAATACATTTCAACTT+GGG | + | Chr7:10085128-10085147 | None:intergenic | 25.0% |
| ! | AATAAGAGACAAACTTTGTT+TGG | + | Chr7:10085687-10085706 | None:intergenic | 25.0% |
| ! | GACAGGATTATTATAAAGTT+TGG | - | Chr7:10085319-10085338 | MsG0780036582.01.T01:intron | 25.0% |
| ! | TACATCATTTAATGATGCAA+TGG | - | Chr7:10084977-10084996 | MsG0780036582.01.T01:intron | 25.0% |
| ! | TGAAATGTATTGTGTTTGAT+AGG | - | Chr7:10085132-10085151 | MsG0780036582.01.T01:intron | 25.0% |
| ! | TTAAATATAACCACATGAGT+CGG | + | Chr7:10085561-10085580 | None:intergenic | 25.0% |
| !! | AAAAAAAACAGAGGATTAGT+AGG | + | Chr7:10085940-10085959 | None:intergenic | 25.0% |
| !! | AATAATTTGACTTATGTTGG+TGG | - | Chr7:10085185-10085204 | MsG0780036582.01.T01:intron | 25.0% |
| !! | AGCAATAATTTGACTTATGT+TGG | - | Chr7:10085182-10085201 | MsG0780036582.01.T01:intron | 25.0% |
| !! | ATAATTTGACTTATGTTGGT+GGG | - | Chr7:10085186-10085205 | MsG0780036582.01.T01:intron | 25.0% |
| !!! | TGAATTTTTGGTTTGTGTTT+TGG | + | Chr7:10084463-10084482 | None:intergenic | 25.0% |
| AAAGAAGCTGTTACAAATGA+TGG | - | Chr7:10086042-10086061 | MsG0780036582.01.T01:CDS | 30.0% | |
| AAGAAGCTGTTACAAATGAT+GGG | - | Chr7:10086043-10086062 | MsG0780036582.01.T01:CDS | 30.0% | |
| AAGGAGATATCTTATAGTCT+TGG | + | Chr7:10086230-10086249 | None:intergenic | 30.0% | |
| ACTAAGTTTGAGAGAGAAAT+TGG | + | Chr7:10084648-10084667 | None:intergenic | 30.0% | |
| AGATTCCATGTCTCTTAAAT+GGG | - | Chr7:10084578-10084597 | MsG0780036582.01.T01:CDS | 30.0% | |
| CAGACATATATGAGATACAT+AGG | + | Chr7:10085529-10085548 | None:intergenic | 30.0% | |
| TAATGGAAAAGAGAGAAATG+AGG | - | Chr7:10086170-10086189 | MsG0780036582.01.T01:CDS | 30.0% | |
| TACTCAAATTAAGCTCCTTA+TGG | - | Chr7:10086128-10086147 | MsG0780036582.01.T01:CDS | 30.0% | |
| TAGATTCCATGTCTCTTAAA+TGG | - | Chr7:10084577-10084596 | MsG0780036582.01.T01:CDS | 30.0% | |
| TATAAGATATCTCCTTTGAC+TGG | - | Chr7:10086234-10086253 | MsG0780036582.01.T01:exon | 30.0% | |
| TATCCTTATGCAATCCTTAT+CGG | + | Chr7:10086010-10086029 | None:intergenic | 30.0% | |
| TTTACATAATTGGACCGATA+AGG | - | Chr7:10085993-10086012 | MsG0780036582.01.T01:CDS | 30.0% | |
| ! | GATTGTGATTTTGTGTTTGA+TGG | - | Chr7:10085035-10085054 | MsG0780036582.01.T01:intron | 30.0% |
| ! | TAATTTGACTTATGTTGGTG+GGG | - | Chr7:10085187-10085206 | MsG0780036582.01.T01:intron | 30.0% |
| !! | TGATTTTGTGTTTGATGGAT+TGG | - | Chr7:10085040-10085059 | MsG0780036582.01.T01:intron | 30.0% |
| !! | TGTGCTTTGAAAATTTCACT+TGG | + | Chr7:10084525-10084544 | None:intergenic | 30.0% |
| !!! | TACTTTAGGATCTGGTTTTT+GGG | - | Chr7:10084926-10084945 | MsG0780036582.01.T01:intron | 30.0% |
| !!! | TTACTTTAGGATCTGGTTTT+TGG | - | Chr7:10084925-10084944 | MsG0780036582.01.T01:intron | 30.0% |
| AAGAAACTCTTCATAGAAGC+TGG | - | Chr7:10086201-10086220 | MsG0780036582.01.T01:CDS | 35.0% | |
| AAGAGACATGGAATCTATGA+AGG | + | Chr7:10084574-10084593 | None:intergenic | 35.0% | |
| AAGAGAGAAATGAGGAAGAA+TGG | - | Chr7:10086178-10086197 | MsG0780036582.01.T01:CDS | 35.0% | |
| AGAGACAAAAATCCAGTCAA+AGG | + | Chr7:10086249-10086268 | None:intergenic | 35.0% | |
| CAAGTTGTAGAGAACTTATC+TGG | - | Chr7:10085158-10085177 | MsG0780036582.01.T01:intron | 35.0% | |
| CCAAACATAATCCAAAACCA+TGG | - | Chr7:10084618-10084637 | MsG0780036582.01.T01:CDS | 35.0% | |
| GATAAGTTCTCTACAACTTG+TGG | + | Chr7:10085158-10085177 | None:intergenic | 35.0% | |
| TATGAGATACATAGGTGTAG+TGG | + | Chr7:10085521-10085540 | None:intergenic | 35.0% | |
| TGTTCTTGATCCAACTCTTT+CGG | - | Chr7:10084845-10084864 | MsG0780036582.01.T01:intron | 35.0% | |
| TTGGAATCAATTGTGGATGT+TGG | - | Chr7:10085059-10085078 | MsG0780036582.01.T01:intron | 35.0% | |
| ! | GCTGAAGAAATGGATTTATG+AGG | - | Chr7:10084881-10084900 | MsG0780036582.01.T01:intron | 35.0% |
| ! | GTAAACATGGCTTGTCTTAA+TGG | - | Chr7:10086153-10086172 | MsG0780036582.01.T01:CDS | 35.0% |
| ! | GTGAAAAAGTGATGACGTTA+TGG | + | Chr7:10085819-10085838 | None:intergenic | 35.0% |
| ! | GTTACAAATGATGGGAAAAG+AGG | - | Chr7:10086051-10086070 | MsG0780036582.01.T01:CDS | 35.0% |
| ! | TGATCTTTGTTTAGCACCAA+TGG | - | Chr7:10084815-10084834 | MsG0780036582.01.T01:intron | 35.0% |
| !! | ACACTCTTTGGTGTTACTTT+AGG | - | Chr7:10084912-10084931 | MsG0780036582.01.T01:intron | 35.0% |
| !! | TGATGGATTGGAATCAATTG+TGG | - | Chr7:10085052-10085071 | MsG0780036582.01.T01:intron | 35.0% |
| !! | TTTGGTGTTACTTTAGGATC+TGG | - | Chr7:10084918-10084937 | MsG0780036582.01.T01:intron | 35.0% |
| !!! | AAATTGGTTTGCCATGGTTT+TGG | + | Chr7:10084632-10084651 | None:intergenic | 35.0% |
| !!! | CCATGGTTTTGGATTATGTT+TGG | + | Chr7:10084621-10084640 | None:intergenic | 35.0% |
| AACAGCCCATTTAAGAGACA+TGG | + | Chr7:10084586-10084605 | None:intergenic | 40.0% | |
| AAGCCATGTTTACGTCCATA+AGG | + | Chr7:10086146-10086165 | None:intergenic | 40.0% | |
| AGCAAAACTGCATCAGCATT+AGG | + | Chr7:10085230-10085249 | None:intergenic | 40.0% | |
| ATTCTACACCTGTGCAATAG+CGG | - | Chr7:10085295-10085314 | MsG0780036582.01.T01:intron | 40.0% | |
| CAAGTTCCATCGTCGAAAAT+AGG | - | Chr7:10084678-10084697 | MsG0780036582.01.T01:CDS | 40.0% | |
| CGTATCATGAGCTGAAGAAA+TGG | - | Chr7:10084871-10084890 | MsG0780036582.01.T01:intron | 40.0% | |
| GAATCAATTGTGGATGTTGG+TGG | - | Chr7:10085062-10085081 | MsG0780036582.01.T01:intron | 40.0% | |
| GAGGAAGATCTTACACTCTT+TGG | - | Chr7:10084900-10084919 | MsG0780036582.01.T01:intron | 40.0% | |
| GTTCTTGATCCAACTCTTTC+GGG | - | Chr7:10084846-10084865 | MsG0780036582.01.T01:intron | 40.0% | |
| TCAAGAACACACTCAACCAT+TGG | + | Chr7:10084834-10084853 | None:intergenic | 40.0% | |
| ! | ACGTTACCTATTTTCGACGA+TGG | + | Chr7:10084687-10084706 | None:intergenic | 40.0% |
| ! | TGCTGATGCAGTTTTGCTTA+AGG | - | Chr7:10085232-10085251 | MsG0780036582.01.T01:intron | 40.0% |
| !! | AGAGAGAAATTGGTTTGCCA+TGG | + | Chr7:10084638-10084657 | None:intergenic | 40.0% |
| CGTCGAAAATAGGTAACGTG+CGG | - | Chr7:10084688-10084707 | MsG0780036582.01.T01:CDS | 45.0% | |
| GAGATACATAGGTGTAGTGG+CGG | + | Chr7:10085518-10085537 | None:intergenic | 45.0% | |
| GCTCCTTATGGACGTAAACA+TGG | - | Chr7:10086140-10086159 | MsG0780036582.01.T01:CDS | 45.0% | |
| GCTTCAGAGCTTCTTGTTAG+AGG | - | Chr7:10084789-10084808 | MsG0780036582.01.T01:intron | 45.0% | |
| GGACCGATAAGGATTGCATA+AGG | - | Chr7:10086004-10086023 | MsG0780036582.01.T01:CDS | 45.0% | |
| TGATACGAACCCGAAAGAGT+TGG | + | Chr7:10084858-10084877 | None:intergenic | 45.0% | |
| ! | TCAATTGTGGATGTTGGTGG+TGG | - | Chr7:10085065-10085084 | MsG0780036582.01.T01:intron | 45.0% |
| TAGGTGTAGTGGCGGTTCTA+AGG | + | Chr7:10085510-10085529 | None:intergenic | 50.0% | |
| !! | GTGTTCAACACCGACTCATG+TGG | - | Chr7:10085548-10085567 | MsG0780036582.01.T01:CDS | 50.0% |
| ATGCGTTACCTCGCGCACAA+CGG | - | Chr7:10084717-10084736 | MsG0780036582.01.T01:intron | 55.0% | |
| CACAGCACTTACGCAAGCAC+GGG | + | Chr7:10085626-10085645 | None:intergenic | 55.0% | |
| GCACAGCACTTACGCAAGCA+CGG | + | Chr7:10085627-10085646 | None:intergenic | 55.0% | |
| TCCTGTCTCCGCTATTGCAC+AGG | + | Chr7:10085306-10085325 | None:intergenic | 55.0% | |
| ! | ACCTGTGCAATAGCGGAGAC+AGG | - | Chr7:10085302-10085321 | MsG0780036582.01.T01:intron | 55.0% |
| !! | GTGGATGTTGGTGGTGGAAC+TGG | - | Chr7:10085071-10085090 | MsG0780036582.01.T01:intron | 55.0% |
| CGAAGAATCCGTTGTGCGCG+AGG | + | Chr7:10084728-10084747 | None:intergenic | 60.0% | |
| !! | GTAGTGGCGGTTCTAAGGCC+CGG | + | Chr7:10085505-10085524 | None:intergenic | 60.0% |
| !! | TAGTGGCGGTTCTAAGGCCC+GGG | + | Chr7:10085504-10085523 | None:intergenic | 60.0% |
| !! | TTCTAAGGCCCGGGGAGCTT+GGG | + | Chr7:10085495-10085514 | None:intergenic | 60.0% |
| !! | AGTGGCGGTTCTAAGGCCCG+GGG | + | Chr7:10085503-10085522 | None:intergenic | 65.0% |
| !! | GTTCTAAGGCCCGGGGAGCT+TGG | + | Chr7:10085496-10085515 | None:intergenic | 65.0% |
| GGCCAGAGCCCAAGCTCCCC+GGG | - | Chr7:10085484-10085503 | MsG0780036582.01.T01:intron | 75.0% | |
| GGGCCAGAGCCCAAGCTCCC+CGG | - | Chr7:10085483-10085502 | MsG0780036582.01.T01:intron | 75.0% | |
| ! | GGCCCGGGGAGCTTGGGCTC+TGG | + | Chr7:10085489-10085508 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 10084458 | 10086287 | 10084458 | ID=MsG0780036582.01;Name=MsG0780036582.01 |
| Chr7 | mRNA | 10084458 | 10086287 | 10084458 | ID=MsG0780036582.01.T01;Parent=MsG0780036582.01;Name=MsG0780036582.01.T01;_AED=0.36;_eAED=0.37;_QI=37|0|0.5|1|1|1|2|0|343 |
| Chr7 | exon | 10084458 | 10084730 | 10084458 | ID=MsG0780036582.01.T01:exon:10388;Parent=MsG0780036582.01.T01 |
| Chr7 | exon | 10085492 | 10086287 | 10085492 | ID=MsG0780036582.01.T01:exon:10387;Parent=MsG0780036582.01.T01 |
| Chr7 | five_prime_UTR | 10086251 | 10086287 | 10086251 | ID=MsG0780036582.01.T01:five_prime_utr;Parent=MsG0780036582.01.T01 |
| Chr7 | CDS | 10085492 | 10086250 | 10085492 | ID=MsG0780036582.01.T01:cds;Parent=MsG0780036582.01.T01 |
| Chr7 | CDS | 10084458 | 10084730 | 10084458 | ID=MsG0780036582.01.T01:cds;Parent=MsG0780036582.01.T01 |
| Gene Sequence |
| Protein sequence |
