Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007258.01.T01 | XP_013462848.1 | 94.712 | 208 | 6 | 1 | 1 | 208 | 229 | 431 | 1.35E-137 | 402 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007258.01.T01 | O65451 | 50.718 | 209 | 82 | 4 | 1 | 208 | 230 | 418 | 1.10E-62 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007258.01.T01 | A0A072V6D3 | 94.712 | 208 | 6 | 1 | 1 | 208 | 229 | 431 | 6.45e-138 | 402 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048035.01 | MsG0280007258.01 | 0.810090 | 1.370020e-50 | 8.183077e-48 |
| MsG0080048177.01 | MsG0280007258.01 | 0.819029 | 1.439044e-52 | 1.095001e-49 |
| MsG0180000157.01 | MsG0280007258.01 | 0.809272 | 2.053927e-50 | 1.200612e-47 |
| MsG0180000197.01 | MsG0280007258.01 | 0.813215 | 2.865557e-51 | 1.859855e-48 |
| MsG0180000585.01 | MsG0280007258.01 | 0.833506 | 5.127333e-56 | 5.916774e-53 |
| MsG0180000673.01 | MsG0280007258.01 | 0.846153 | 2.596743e-59 | 4.428200e-56 |
| MsG0180000821.01 | MsG0280007258.01 | 0.822123 | 2.803455e-53 | 2.325426e-50 |
| MsG0180000822.01 | MsG0280007258.01 | 0.815807 | 7.652414e-52 | 5.329565e-49 |
| MsG0180001043.01 | MsG0280007258.01 | 0.832867 | 7.398904e-56 | 8.376415e-53 |
| MsG0180001154.01 | MsG0280007258.01 | 0.806306 | 8.771193e-50 | 4.744754e-47 |
| MsG0180001355.01 | MsG0280007258.01 | 0.804075 | 2.570782e-49 | 1.313251e-46 |
| MsG0180001921.01 | MsG0280007258.01 | 0.824271 | 8.835122e-54 | 7.788026e-51 |
| MsG0180001922.01 | MsG0280007258.01 | 0.829443 | 5.134272e-55 | 5.255349e-52 |
| MsG0180002075.01 | MsG0280007258.01 | 0.806092 | 9.729063e-50 | 5.234356e-47 |
| MsG0180002912.01 | MsG0280007258.01 | 0.808930 | 2.431543e-50 | 1.408283e-47 |
| MsG0180003088.01 | MsG0280007258.01 | 0.801821 | 7.515770e-49 | 3.624172e-46 |
| MsG0180003241.01 | MsG0280007258.01 | 0.854468 | 1.206456e-61 | 2.697036e-58 |
| MsG0180003252.01 | MsG0280007258.01 | 0.809375 | 1.952363e-50 | 1.144388e-47 |
| MsG0180004388.01 | MsG0280007258.01 | 0.827565 | 1.458673e-54 | 1.413303e-51 |
| MsG0180004762.01 | MsG0280007258.01 | 0.812207 | 4.761531e-51 | 3.008732e-48 |
| MsG0180005125.01 | MsG0280007258.01 | 0.820002 | 8.632979e-53 | 6.748929e-50 |
| MsG0180005944.01 | MsG0280007258.01 | 0.801019 | 1.097052e-48 | 5.184345e-46 |
| MsG0180006016.01 | MsG0280007258.01 | 0.815393 | 9.462178e-52 | 6.515487e-49 |
| MsG0280006728.01 | MsG0280007258.01 | 0.803988 | 2.680348e-49 | 1.366176e-46 |
| MsG0280006935.01 | MsG0280007258.01 | 0.800759 | 1.239537e-48 | 5.818653e-46 |
| MsG0280007258.01 | MsG0280007272.01 | 0.824176 | 9.301351e-54 | 8.177506e-51 |
| MsG0280007258.01 | MsG0280007439.01 | 0.816958 | 4.227355e-52 | 3.038678e-49 |
| MsG0280007258.01 | MsG0280007481.01 | 0.807864 | 4.104008e-50 | 2.311289e-47 |
| MsG0280007258.01 | MsG0280007549.01 | 0.824508 | 7.770481e-54 | 6.896678e-51 |
| MsG0280007258.01 | MsG0280007585.01 | 0.804888 | 1.740455e-49 | 9.079588e-47 |
| MsG0280007258.01 | MsG0280007948.01 | 0.812763 | 3.599803e-51 | 2.308415e-48 |
| MsG0280007258.01 | MsG0280008220.01 | 0.856182 | 3.823539e-62 | 9.048098e-59 |
| MsG0280007258.01 | MsG0280008295.01 | 0.814390 | 1.578729e-51 | 1.058004e-48 |
| MsG0280007258.01 | MsG0280009165.01 | 0.819067 | 1.411009e-52 | 1.074688e-49 |
| MsG0280007258.01 | MsG0280009598.01 | 0.811188 | 7.931475e-51 | 4.877321e-48 |
| MsG0280007258.01 | MsG0280010170.01 | 0.808329 | 3.266895e-50 | 1.862715e-47 |
| MsG0280007258.01 | MsG0280010739.01 | 0.808651 | 2.788573e-50 | 1.603275e-47 |
| MsG0280007258.01 | MsG0280011144.01 | 0.804264 | 2.347987e-49 | 1.205206e-46 |
| MsG0280007258.01 | MsG0380011754.01 | 0.854949 | 8.754968e-62 | 1.988556e-58 |
| MsG0280007258.01 | MsG0380014680.01 | 0.805819 | 1.110196e-49 | 5.931539e-47 |
| MsG0280007258.01 | MsG0380015181.01 | 0.827680 | 1.368849e-54 | 1.330769e-51 |
| MsG0280007258.01 | MsG0380015686.01 | 0.832457 | 9.349470e-56 | 1.045578e-52 |
| MsG0280007258.01 | MsG0380015931.01 | 0.817700 | 2.878592e-52 | 2.111427e-49 |
| MsG0280007258.01 | MsG0380017158.01 | 0.835152 | 1.981062e-56 | 2.401814e-53 |
| MsG0280007258.01 | MsG0380017286.01 | 0.863666 | 2.114729e-64 | 6.462687e-61 |
| MsG0280007258.01 | MsG0380017631.01 | 0.808635 | 2.810887e-50 | 1.615410e-47 |
| MsG0280007258.01 | MsG0380017940.01 | 0.813352 | 2.673322e-51 | 1.741749e-48 |
| MsG0280007258.01 | MsG0480018160.01 | 0.807904 | 4.025356e-50 | 2.269248e-47 |
| MsG0280007258.01 | MsG0480018321.01 | 0.806013 | 1.010799e-49 | 5.427362e-47 |
| MsG0280007258.01 | MsG0480018904.01 | 0.810392 | 1.179263e-50 | 7.099487e-48 |
| MsG0280007258.01 | MsG0480020242.01 | 0.812955 | 3.268017e-51 | 2.106223e-48 |
| MsG0280007258.01 | MsG0480020445.01 | 0.834452 | 2.972505e-56 | 3.529254e-53 |
| MsG0280007258.01 | MsG0480020487.01 | 0.819852 | 9.340121e-53 | 7.271168e-50 |
| MsG0280007258.01 | MsG0480021240.01 | 0.821295 | 4.357293e-53 | 3.530750e-50 |
| MsG0280007258.01 | MsG0480022274.01 | 0.800196 | 1.614690e-48 | 7.472120e-46 |
| MsG0280007258.01 | MsG0480022479.01 | 0.810385 | 1.183161e-50 | 7.121645e-48 |
| MsG0280007258.01 | MsG0480022480.01 | 0.824356 | 8.435969e-54 | 7.454407e-51 |
| MsG0280007258.01 | MsG0480022775.01 | 0.802913 | 4.476023e-49 | 2.219333e-46 |
| MsG0280007258.01 | MsG0480023224.01 | 0.814839 | 1.255554e-51 | 8.516137e-49 |
| MsG0280007258.01 | MsG0480023245.01 | 0.802871 | 4.567254e-49 | 2.262094e-46 |
| MsG0280007258.01 | MsG0480023280.01 | 0.806286 | 8.853967e-50 | 4.787211e-47 |
| MsG0280007258.01 | MsG0480023417.01 | 0.835348 | 1.767837e-56 | 2.156229e-53 |
| MsG0280007258.01 | MsG0480023427.01 | 0.806815 | 6.848721e-50 | 3.753481e-47 |
| MsG0280007258.01 | MsG0480023517.01 | 0.863377 | 2.600168e-64 | 7.865625e-61 |
| MsG0280007258.01 | MsG0480023903.01 | 0.835818 | 1.344564e-56 | 1.662991e-53 |
| MsG0280007258.01 | MsG0580024073.01 | 0.817854 | 2.657599e-52 | 1.957611e-49 |
| MsG0280007258.01 | MsG0580024114.01 | 0.821887 | 3.178639e-53 | 2.619073e-50 |
| MsG0280007258.01 | MsG0580024205.01 | 0.801944 | 7.088859e-49 | 3.428958e-46 |
| MsG0280007258.01 | MsG0580025278.01 | 0.821622 | 3.661701e-53 | 2.994281e-50 |
| MsG0280007258.01 | MsG0580025453.01 | 0.824304 | 8.678860e-54 | 7.657332e-51 |
| MsG0280007258.01 | MsG0580025769.01 | 0.843603 | 1.265598e-58 | 1.990589e-55 |
| MsG0280007258.01 | MsG0580027129.01 | 0.828473 | 8.815228e-55 | 8.771122e-52 |
| MsG0280007258.01 | MsG0580027345.01 | 0.819329 | 1.229817e-52 | 9.434347e-50 |
| MsG0280007258.01 | MsG0580027628.01 | 0.802766 | 4.801460e-49 | 2.371816e-46 |
| MsG0280007258.01 | MsG0580027773.01 | 0.802722 | 4.902863e-49 | 2.419195e-46 |
| MsG0280007258.01 | MsG0580028185.01 | 0.826130 | 3.212379e-54 | 2.986300e-51 |
| MsG0280007258.01 | MsG0580029148.01 | 0.804963 | 1.678375e-49 | 8.772169e-47 |
| MsG0280007258.01 | MsG0580029658.01 | 0.807618 | 4.628981e-50 | 2.590312e-47 |
| MsG0280007258.01 | MsG0580029758.01 | 0.823283 | 1.505882e-53 | 1.290921e-50 |
| MsG0280007258.01 | MsG0580030235.01 | 0.809349 | 1.977139e-50 | 1.158146e-47 |
| MsG0280007258.01 | MsG0680030303.01 | 0.809409 | 1.919875e-50 | 1.126380e-47 |
| MsG0280007258.01 | MsG0680030685.01 | 0.827347 | 1.645069e-54 | 1.584156e-51 |
| MsG0280007258.01 | MsG0680030841.01 | 0.810676 | 1.024037e-50 | 6.212203e-48 |
| MsG0280007258.01 | MsG0680031459.01 | 0.845344 | 4.307291e-59 | 7.156458e-56 |
| MsG0280007258.01 | MsG0680031538.01 | 0.806447 | 8.191827e-50 | 4.447289e-47 |
| MsG0280007258.01 | MsG0680031694.01 | 0.806885 | 6.618887e-50 | 3.634072e-47 |
| MsG0280007258.01 | MsG0680031719.01 | 0.835092 | 2.051604e-56 | 2.482661e-53 |
| MsG0280007258.01 | MsG0680034450.01 | 0.816425 | 5.568419e-52 | 3.944106e-49 |
| MsG0280007258.01 | MsG0680034672.01 | 0.850460 | 1.674557e-60 | 3.282441e-57 |
| MsG0280007258.01 | MsG0680035582.01 | 0.811431 | 7.026460e-51 | 4.348691e-48 |
| MsG0280007258.01 | MsG0780036235.01 | 0.803669 | 3.121421e-49 | 1.577881e-46 |
| MsG0280007258.01 | MsG0780036291.01 | 0.857749 | 1.320514e-62 | 3.293365e-59 |
| MsG0280007258.01 | MsG0780036550.01 | 0.805713 | 1.169024e-49 | 6.228960e-47 |
| MsG0280007258.01 | MsG0780036582.01 | 0.829360 | 5.377792e-55 | 5.492018e-52 |
| MsG0280007258.01 | MsG0780037415.01 | 0.824846 | 6.469237e-54 | 5.798164e-51 |
| MsG0280007258.01 | MsG0780037503.01 | 0.803369 | 3.602067e-49 | 1.806857e-46 |
| MsG0280007258.01 | MsG0780038444.01 | 0.844997 | 5.344851e-59 | 8.782376e-56 |
| MsG0280007258.01 | MsG0780038495.01 | 0.810041 | 1.403630e-50 | 8.373778e-48 |
| MsG0280007258.01 | MsG0780040516.01 | 0.811315 | 7.445960e-51 | 4.593896e-48 |
| MsG0280007258.01 | MsG0780040679.01 | 0.835772 | 1.381525e-56 | 1.706340e-53 |
| MsG0280007258.01 | MsG0780040726.01 | 0.813245 | 2.822610e-51 | 1.833489e-48 |
| MsG0280007258.01 | MsG0780040758.01 | 0.815616 | 8.439956e-52 | 5.847954e-49 |
| MsG0280007258.01 | MsG0780041196.01 | 0.802099 | 6.589206e-49 | 3.199895e-46 |
| MsG0280007258.01 | MsG0880042611.01 | 0.813318 | 2.719505e-51 | 1.770155e-48 |
| MsG0280007258.01 | MsG0880042843.01 | 0.829012 | 6.531037e-55 | 6.600688e-52 |
| MsG0280007258.01 | MsG0880043001.01 | 0.826544 | 2.559597e-54 | 2.407565e-51 |
| MsG0280007258.01 | MsG0880043082.01 | 0.803717 | 3.050622e-49 | 1.544002e-46 |
| MsG0280007258.01 | MsG0880043279.01 | 0.807717 | 4.409719e-50 | 2.474039e-47 |
| MsG0280007258.01 | MsG0880043288.01 | 0.833218 | 6.049433e-56 | 6.921236e-53 |
| MsG0280007258.01 | MsG0880043489.01 | 0.801442 | 8.987194e-49 | 4.292388e-46 |
| MsG0280007258.01 | MsG0880044039.01 | 0.813863 | 2.063804e-51 | 1.363369e-48 |
| MsG0280007258.01 | MsG0880045106.01 | 0.846669 | 1.878308e-59 | 3.257579e-56 |
| MsG0280007258.01 | MsG0880045803.01 | 0.803517 | 3.357707e-49 | 1.690773e-46 |
| MsG0280007258.01 | MsG0880045914.01 | 0.803000 | 4.295865e-49 | 2.134557e-46 |
| MsG0280007258.01 | MsG0880046058.01 | 0.816559 | 5.194971e-52 | 3.693245e-49 |
| MsG0280007258.01 | MsG0880046404.01 | 0.803270 | 3.776968e-49 | 1.889812e-46 |
| MsG0280007258.01 | MsG0880046440.01 | 0.807338 | 5.307065e-50 | 2.948320e-47 |
| MsG0280007258.01 | MsG0880046586.01 | 0.804951 | 1.687876e-49 | 8.819290e-47 |
| MsG0280007258.01 | MsG0880046642.01 | 0.819320 | 1.235588e-52 | 9.476189e-50 |
| MsG0280007258.01 | MsG0880046719.01 | 0.821159 | 4.682093e-53 | 3.779792e-50 |
| MsG0280007258.01 | MsG0880046731.01 | 0.870623 | 1.269009e-66 | 4.969197e-63 |
| MsG0280007258.01 | MsG0880046733.01 | 0.819458 | 1.149458e-52 | 8.850691e-50 |
| MsG0280007258.01 | MsG0880046888.01 | 0.824154 | 9.411107e-54 | 8.268847e-51 |
| MsG0280007258.01 | MsG0880047143.01 | 0.818111 | 2.324950e-52 | 1.724641e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007258.01.T01 | MTR_2g023650 | 94.712 | 208 | 6 | 1 | 1 | 208 | 229 | 431 | 1.64e-141 | 402 |
| MsG0280007258.01.T01 | MTR_2g023730 | 94.712 | 208 | 6 | 1 | 1 | 208 | 230 | 432 | 2.25e-141 | 402 |
| MsG0280007258.01.T01 | MTR_2g023790 | 93.750 | 208 | 8 | 1 | 1 | 208 | 228 | 430 | 1.85e-139 | 397 |
| MsG0280007258.01.T01 | MTR_2g023660 | 60.096 | 208 | 73 | 1 | 1 | 208 | 226 | 423 | 4.44e-88 | 266 |
| MsG0280007258.01.T01 | MTR_2g023760 | 58.852 | 209 | 74 | 2 | 1 | 208 | 98 | 295 | 6.02e-87 | 258 |
| MsG0280007258.01.T01 | MTR_2g023820 | 59.135 | 208 | 75 | 1 | 1 | 208 | 227 | 424 | 2.42e-86 | 261 |
| MsG0280007258.01.T01 | MTR_2g023770 | 59.135 | 208 | 75 | 1 | 1 | 208 | 227 | 424 | 2.42e-86 | 261 |
| MsG0280007258.01.T01 | MTR_2g023710 | 57.895 | 209 | 76 | 2 | 1 | 208 | 308 | 505 | 6.95e-84 | 257 |
| MsG0280007258.01.T01 | MTR_4g132390 | 60.000 | 210 | 74 | 3 | 1 | 208 | 227 | 428 | 4.11e-83 | 253 |
| MsG0280007258.01.T01 | MTR_4g132380 | 56.872 | 211 | 78 | 2 | 1 | 208 | 224 | 424 | 1.18e-80 | 247 |
| MsG0280007258.01.T01 | MTR_2g023810 | 58.989 | 178 | 69 | 1 | 31 | 208 | 131 | 304 | 3.39e-73 | 224 |
| MsG0280007258.01.T01 | MTR_2g023750 | 63.253 | 166 | 61 | 0 | 43 | 208 | 8 | 173 | 3.14e-71 | 214 |
| MsG0280007258.01.T01 | MTR_2g023860 | 50.472 | 212 | 86 | 4 | 1 | 208 | 223 | 419 | 3.90e-60 | 194 |
| MsG0280007258.01.T01 | MTR_2g023780 | 55.769 | 156 | 44 | 1 | 53 | 208 | 65 | 195 | 2.93e-57 | 179 |
| MsG0280007258.01.T01 | MTR_2g023800 | 50.538 | 93 | 43 | 1 | 116 | 208 | 1 | 90 | 2.79e-24 | 92.0 |
| MsG0280007258.01.T01 | MTR_2g023830 | 47.436 | 78 | 31 | 1 | 1 | 78 | 226 | 293 | 3.90e-17 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007258.01.T01 | AT4G22030 | 53.110 | 209 | 77 | 4 | 1 | 208 | 230 | 418 | 4.33e-71 | 222 |
| MsG0280007258.01.T01 | AT4G04480 | 48.804 | 209 | 83 | 4 | 1 | 208 | 213 | 398 | 6.21e-63 | 201 |
Find 36 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAATTGGCTTTGAAATT+AGG | 0.160626 | 2:-12685964 | MsG0280007258.01.T01:CDS |
| GATGAAGAACAAAGAGAAAA+TGG | 0.209588 | 2:-12686000 | MsG0280007258.01.T01:CDS |
| AGATTTGTTTGAAATTAAAT+TGG | 0.257786 | 2:-12685977 | MsG0280007258.01.T01:CDS |
| TTTGTTGGCCTCCAATTTGC+AGG | 0.311741 | 2:+12686441 | None:intergenic |
| AGTTATGAAGAGAAAAGATA+TGG | 0.383735 | 2:-12686298 | MsG0280007258.01.T01:CDS |
| GCCAATGCTCCAGCCATAAC+AGG | 0.390698 | 2:+12686144 | None:intergenic |
| AATAAGACATTGGCTATTGC+AGG | 0.415928 | 2:-12686237 | MsG0280007258.01.T01:CDS |
| TGGTGGATTCTTTCAAATGA+TGG | 0.426249 | 2:-12686055 | MsG0280007258.01.T01:CDS |
| ATTTGTTGGTCAAGGTTCAA+TGG | 0.434031 | 2:-12686178 | MsG0280007258.01.T01:CDS |
| GGCATCTATAGTGCCTGTTA+TGG | 0.435733 | 2:-12686157 | MsG0280007258.01.T01:CDS |
| CTCACTGGAATTGCAGCTGT+GGG | 0.436867 | 2:-12686207 | MsG0280007258.01.T01:CDS |
| AAATAATGAAACAAAACAAA+AGG | 0.440479 | 2:-12686379 | MsG0280007258.01.T01:CDS |
| GCCTGTTATGGCTGGAGCAT+TGG | 0.442686 | 2:-12686145 | MsG0280007258.01.T01:CDS |
| GGGTCCACATTTGTTGGTCA+AGG | 0.448877 | 2:-12686186 | MsG0280007258.01.T01:CDS |
| ATGGAAGATTATGAGAGATT+AGG | 0.449631 | 2:-12686279 | MsG0280007258.01.T01:CDS |
| TCAGCTTATTCTCGTGTTGA+GGG | 0.449906 | 2:-12685904 | MsG0280007258.01.T01:CDS |
| TCTATAGTGCCTGTTATGGC+TGG | 0.458826 | 2:-12686153 | MsG0280007258.01.T01:CDS |
| TTAGAGCATGGTGGACAAGT+TGG | 0.481937 | 2:-12686105 | MsG0280007258.01.T01:CDS |
| ATCAGCTTATTCTCGTGTTG+AGG | 0.485175 | 2:-12685905 | MsG0280007258.01.T01:CDS |
| TTATGAGAGATTAGGAAACT+TGG | 0.490965 | 2:-12686271 | MsG0280007258.01.T01:CDS |
| AAGTGAAGTAAAAGATGTGT+TGG | 0.509854 | 2:-12686322 | MsG0280007258.01.T01:CDS |
| GCTGTTAATTCTTTAGAGCA+TGG | 0.511276 | 2:-12686117 | MsG0280007258.01.T01:CDS |
| GCTGTGGGGTCCACATTTGT+TGG | 0.513925 | 2:-12686192 | MsG0280007258.01.T01:CDS |
| ATGAAACAAAACAAAAGGGT+TGG | 0.515061 | 2:-12686374 | MsG0280007258.01.T01:CDS |
| AATAATGAAACAAAACAAAA+GGG | 0.545129 | 2:-12686378 | MsG0280007258.01.T01:CDS |
| ATTGCAGGACCTTTACTCAC+TGG | 0.545526 | 2:-12686222 | MsG0280007258.01.T01:CDS |
| ACTCACTGGAATTGCAGCTG+TGG | 0.560691 | 2:-12686208 | MsG0280007258.01.T01:CDS |
| GCTGCAATTCCAGTGAGTAA+AGG | 0.564619 | 2:+12686213 | None:intergenic |
| AATATGAACCTGCAAATTGG+AGG | 0.566414 | 2:-12686449 | MsG0280007258.01.T01:CDS |
| GTTGAAGATAAATAAGACAT+TGG | 0.583978 | 2:-12686247 | MsG0280007258.01.T01:CDS |
| TCTGAAATGTATAGAGCTTG+TGG | 0.591091 | 2:-12686075 | MsG0280007258.01.T01:CDS |
| TGAACCTTGACCAACAAATG+TGG | 0.592998 | 2:+12686182 | None:intergenic |
| GCATGGTGGACAAGTTGGAA+TGG | 0.601882 | 2:-12686100 | MsG0280007258.01.T01:CDS |
| GTTAATTCTTTAGAGCATGG+TGG | 0.633328 | 2:-12686114 | MsG0280007258.01.T01:CDS |
| TCACTGGAATTGCAGCTGTG+GGG | 0.665725 | 2:-12686206 | MsG0280007258.01.T01:CDS |
| GAAATGTATAGAGCTTGTGG+TGG | 0.683416 | 2:-12686072 | MsG0280007258.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATGAAACAAAACAAA+AGG | - | Chr2:12685959-12685978 | MsG0280007258.01.T01:CDS | 15.0% |
| !! | AATAATGAAACAAAACAAAA+GGG | - | Chr2:12685960-12685979 | MsG0280007258.01.T01:CDS | 15.0% |
| !!! | AGATTTGTTTGAAATTAAAT+TGG | - | Chr2:12686361-12686380 | MsG0280007258.01.T01:CDS | 15.0% |
| !!! | ATTAAATTGGCTTTGAAATT+AGG | - | Chr2:12686374-12686393 | MsG0280007258.01.T01:CDS | 20.0% |
| ! | AAAAATATGAACCTGCAAAT+TGG | - | Chr2:12685886-12685905 | MsG0280007258.01.T01:CDS | 25.0% |
| ! | AGTTATGAAGAGAAAAGATA+TGG | - | Chr2:12686040-12686059 | MsG0280007258.01.T01:CDS | 25.0% |
| ! | GTTGAAGATAAATAAGACAT+TGG | - | Chr2:12686091-12686110 | MsG0280007258.01.T01:CDS | 25.0% |
| !!! | CTTCTTGATGATATTTTTGT+TGG | + | Chr2:12685915-12685934 | None:intergenic | 25.0% |
| AAGAAAAATGGATGGAATGA+AGG | - | Chr2:12685987-12686006 | MsG0280007258.01.T01:CDS | 30.0% | |
| AAGTGAAGTAAAAGATGTGT+TGG | - | Chr2:12686016-12686035 | MsG0280007258.01.T01:CDS | 30.0% | |
| AGAAAAATGGATGGAATGAA+GGG | - | Chr2:12685988-12686007 | MsG0280007258.01.T01:CDS | 30.0% | |
| ATGAAACAAAACAAAAGGGT+TGG | - | Chr2:12685964-12685983 | MsG0280007258.01.T01:CDS | 30.0% | |
| ATGGAAGATTATGAGAGATT+AGG | - | Chr2:12686059-12686078 | MsG0280007258.01.T01:CDS | 30.0% | |
| GATGAAGAACAAAGAGAAAA+TGG | - | Chr2:12686338-12686357 | MsG0280007258.01.T01:CDS | 30.0% | |
| TTATGAGAGATTAGGAAACT+TGG | - | Chr2:12686067-12686086 | MsG0280007258.01.T01:CDS | 30.0% | |
| !! | TTGCAGGTTCATATTTTTGA+GGG | + | Chr2:12685884-12685903 | None:intergenic | 30.0% |
| !! | TTTGCAGGTTCATATTTTTG+AGG | + | Chr2:12685885-12685904 | None:intergenic | 30.0% |
| AATATGAACCTGCAAATTGG+AGG | - | Chr2:12685889-12685908 | MsG0280007258.01.T01:CDS | 35.0% | |
| CAAAAGGGTTGGAAGAAAAA+TGG | - | Chr2:12685975-12685994 | MsG0280007258.01.T01:CDS | 35.0% | |
| ! | AATAAGACATTGGCTATTGC+AGG | - | Chr2:12686101-12686120 | MsG0280007258.01.T01:CDS | 35.0% |
| ! | ATTTGTTGGTCAAGGTTCAA+TGG | - | Chr2:12686160-12686179 | MsG0280007258.01.T01:CDS | 35.0% |
| ! | TCTGAAATGTATAGAGCTTG+TGG | - | Chr2:12686263-12686282 | MsG0280007258.01.T01:CDS | 35.0% |
| !! | GCTGTTAATTCTTTAGAGCA+TGG | - | Chr2:12686221-12686240 | MsG0280007258.01.T01:CDS | 35.0% |
| !! | GTTAATTCTTTAGAGCATGG+TGG | - | Chr2:12686224-12686243 | MsG0280007258.01.T01:CDS | 35.0% |
| !! | TGGTGGATTCTTTCAAATGA+TGG | - | Chr2:12686283-12686302 | MsG0280007258.01.T01:CDS | 35.0% |
| AGGGTTGGAAGAAAAATGGA+TGG | - | Chr2:12685979-12685998 | MsG0280007258.01.T01:CDS | 40.0% | |
| ATCAGCTTATTCTCGTGTTG+AGG | - | Chr2:12686433-12686452 | MsG0280007258.01.T01:CDS | 40.0% | |
| GAAATGTATAGAGCTTGTGG+TGG | - | Chr2:12686266-12686285 | MsG0280007258.01.T01:CDS | 40.0% | |
| TCAGCTTATTCTCGTGTTGA+GGG | - | Chr2:12686434-12686453 | MsG0280007258.01.T01:CDS | 40.0% | |
| TGAACCTTGACCAACAAATG+TGG | + | Chr2:12686159-12686178 | None:intergenic | 40.0% | |
| ATTGCAGGACCTTTACTCAC+TGG | - | Chr2:12686116-12686135 | MsG0280007258.01.T01:CDS | 45.0% | |
| GCTGCAATTCCAGTGAGTAA+AGG | + | Chr2:12686128-12686147 | None:intergenic | 45.0% | |
| GGCATCTATAGTGCCTGTTA+TGG | - | Chr2:12686181-12686200 | MsG0280007258.01.T01:CDS | 45.0% | |
| TCTATAGTGCCTGTTATGGC+TGG | - | Chr2:12686185-12686204 | MsG0280007258.01.T01:CDS | 45.0% | |
| ! | TTTGTTGGCCTCCAATTTGC+AGG | + | Chr2:12685900-12685919 | None:intergenic | 45.0% |
| !! | TTAGAGCATGGTGGACAAGT+TGG | - | Chr2:12686233-12686252 | MsG0280007258.01.T01:CDS | 45.0% |
| ACTCACTGGAATTGCAGCTG+TGG | - | Chr2:12686130-12686149 | MsG0280007258.01.T01:CDS | 50.0% | |
| CTCACTGGAATTGCAGCTGT+GGG | - | Chr2:12686131-12686150 | MsG0280007258.01.T01:CDS | 50.0% | |
| TCACTGGAATTGCAGCTGTG+GGG | - | Chr2:12686132-12686151 | MsG0280007258.01.T01:CDS | 50.0% | |
| ! | GGGTCCACATTTGTTGGTCA+AGG | - | Chr2:12686152-12686171 | MsG0280007258.01.T01:CDS | 50.0% |
| !! | GCATGGTGGACAAGTTGGAA+TGG | - | Chr2:12686238-12686257 | MsG0280007258.01.T01:CDS | 50.0% |
| GCCAATGCTCCAGCCATAAC+AGG | + | Chr2:12686197-12686216 | None:intergenic | 55.0% | |
| GCCTGTTATGGCTGGAGCAT+TGG | - | Chr2:12686193-12686212 | MsG0280007258.01.T01:CDS | 55.0% | |
| GCTGTGGGGTCCACATTTGT+TGG | - | Chr2:12686146-12686165 | MsG0280007258.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 12685867 | 12686493 | 12685867 | ID=MsG0280007258.01;Name=MsG0280007258.01 |
| Chr2 | mRNA | 12685867 | 12686493 | 12685867 | ID=MsG0280007258.01.T01;Parent=MsG0280007258.01;Name=MsG0280007258.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|208 |
| Chr2 | exon | 12685867 | 12686493 | 12685867 | ID=MsG0280007258.01.T01:exon:518;Parent=MsG0280007258.01.T01 |
| Chr2 | CDS | 12685867 | 12686493 | 12685867 | ID=MsG0280007258.01.T01:cds;Parent=MsG0280007258.01.T01 |
| Gene Sequence |
| Protein sequence |
