AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042944.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042944.01.T01 MTR_8g026680 84.306 497 32 4 1 456 1 492 0.0 787
MsG0880042944.01.T01 MTR_7g104900 66.154 260 77 2 87 335 69 328 1.59e-123 368
MsG0880042944.01.T01 MTR_1g089500 61.149 296 98 3 76 354 23 318 2.17e-122 365
MsG0880042944.01.T01 MTR_2g086580 64.815 270 82 3 84 340 42 311 4.01e-119 356
MsG0880042944.01.T01 MTR_4g052850 57.483 294 100 4 84 366 65 344 1.12e-114 341
MsG0880042944.01.T01 MTR_7g107560 65.251 259 74 3 92 334 32 290 5.72e-108 327
MsG0880042944.01.T01 MTR_7g081615 59.494 158 56 1 153 302 14 171 4.45e-61 197
MsG0880042944.01.T01 MTR_5g064420 53.757 173 60 3 134 296 33 195 5.94e-53 178
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042944.01.T01 AT2G46710 52.083 480 173 13 1 456 1 447 2.27e-147 428
MsG0880042944.01.T01 AT5G22400 66.154 260 78 1 86 335 87 346 4.83e-124 369
MsG0880042944.01.T01 AT3G11490 61.168 291 97 2 90 364 67 357 4.68e-122 363
MsG0880042944.01.T01 AT4G03100 63.941 269 75 3 87 333 39 307 3.09e-120 358
MsG0880042944.01.T01 AT1G08340 65.106 235 64 2 114 334 1 231 3.29e-109 326

Find 100 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAACCAGTTTGCAATAT+TGG 0.157516 8:-16386372 MsG0880042944.01.T01:CDS
TTCATCTTTCTTCTTGTTGA+TGG 0.175582 8:+16386541 None:intergenic
TTGTTTATCCGGCTTGATTA+AGG 0.253081 8:-16384966 MsG0880042944.01.T01:intron
TGATGCAGTGTGAAGGTATT+TGG 0.306995 8:-16385296 MsG0880042944.01.T01:intron
TTTACAATGGCAAGACTTGC+AGG 0.317689 8:+16383302 None:intergenic
TAGAGAGAAAATGAAGAGTT+TGG 0.320950 8:+16386830 None:intergenic
ATGAAAATGTGATATGGTTT+TGG 0.329026 8:+16386761 None:intergenic
CTTCGGAGCTCCGAGGAAAA+AGG 0.333309 8:-16383511 MsG0880042944.01.T01:CDS
GATCTCGAACGAATGCTTCT+TGG 0.347459 8:+16385029 None:intergenic
ACTTGAATGAAAATGTGATA+TGG 0.353168 8:+16386755 None:intergenic
ATCGATGTTCATTGTTTATC+CGG 0.358798 8:-16384977 MsG0880042944.01.T01:CDS
TTGATGGTTACTTGTGCAAC+AGG 0.375336 8:+16386557 None:intergenic
AGACTTGCAGGCTTCTTAGA+TGG 0.380145 8:+16383314 None:intergenic
GGGAAATTATGAAAGACAAT+TGG 0.381230 8:+16386859 None:intergenic
GAGGGAAGATGTTTCTTCCT+TGG 0.381917 8:-16386300 MsG0880042944.01.T01:CDS
TGACTGGGCAATCAATTTGA+TGG 0.385583 8:-16384292 MsG0880042944.01.T01:CDS
CAGAAGCTGCTCTTCTTGAC+TGG 0.401025 8:-16384308 MsG0880042944.01.T01:CDS
TGCATGAATCAATGCAGTTA+AGG 0.415964 8:+16383792 None:intergenic
GCATGAATCAATGCAGTTAA+GGG 0.418194 8:+16383793 None:intergenic
AGGAGGAAATTCATCATTGC+AGG 0.421427 8:+16383663 None:intergenic
ATTATGAAAGACAATTGGAT+TGG 0.424236 8:+16386864 None:intergenic
TTTCTTCCTTGGATATTAGC+TGG 0.437424 8:-16386289 MsG0880042944.01.T01:CDS
GGCTTGCTCAATTGAAATAC+CGG 0.440128 8:+16383335 None:intergenic
CTATATCCAATATTGCAAAC+TGG 0.444268 8:+16386366 None:intergenic
GTCTAATTTCATGCTTTCCA+TGG 0.445413 8:-16385093 MsG0880042944.01.T01:CDS
TCCTCTTTGTTGAAGTTGAA+AGG 0.462988 8:+16383643 None:intergenic
TGGTTTAGAGAGCTTCCAAC+TGG 0.465706 8:-16384417 MsG0880042944.01.T01:CDS
ATTCCTTCTGCCTTCATCCA+TGG 0.472811 8:+16385076 None:intergenic
CTGGCACTAGGTACTCTTGT+AGG 0.477302 8:+16386179 None:intergenic
CAGTGTTCTTATGATGATAG+AGG 0.479488 8:-16385254 MsG0880042944.01.T01:CDS
TTCGAGATCAGTTAAACAAA+GGG 0.480003 8:-16385015 MsG0880042944.01.T01:CDS
TTATGAAAGACAATTGGATT+GGG 0.480560 8:+16386865 None:intergenic
GGAAGGAAGAGGAGAAGCTT+GGG 0.485623 8:-16383273 MsG0880042944.01.T01:CDS
GCTCCATTCTATTCTTCCTA+AGG 0.486204 8:+16383543 None:intergenic
AATGAAGAGTTTGGTTGAGA+GGG 0.486933 8:+16386839 None:intergenic
TTCATGCTTTCCATGGATGA+AGG 0.489913 8:-16385086 MsG0880042944.01.T01:CDS
GGGAAGGAAGAGGAGAAGCT+TGG 0.491896 8:-16383274 MsG0880042944.01.T01:CDS
GTCAAGAAGAGCAGCTTCTG+TGG 0.495179 8:+16384311 None:intergenic
ACGGCGTGCGCAAGTTATGC+AGG 0.495303 8:-16383361 MsG0880042944.01.T01:CDS
GCCTTCTGAGTTTCAGCCTG+AGG 0.495415 8:-16386204 MsG0880042944.01.T01:CDS
TTCTCAAGGACTTCCACCTT+AGG 0.495514 8:-16383559 MsG0880042944.01.T01:CDS
GTTTCAAGACAATTCACAAA+AGG 0.497631 8:-16386410 MsG0880042944.01.T01:CDS
GAATGCGCGCAACGTAGCTA+TGG 0.499082 8:-16384235 MsG0880042944.01.T01:CDS
GTCTTGCCATTGTAAATACT+AGG 0.506258 8:-16383295 MsG0880042944.01.T01:CDS
TCAAAATCATGCAACATAGC+TGG 0.506873 8:-16386602 MsG0880042944.01.T01:CDS
AAATGAAGAGTTTGGTTGAG+AGG 0.509274 8:+16386838 None:intergenic
TCCTTTCAACTTCAACAAAG+AGG 0.511319 8:-16383644 MsG0880042944.01.T01:CDS
GGTACTCTTGTAGGAACCTC+AGG 0.515998 8:+16386188 None:intergenic
TAACAAACCTGTGTCATGTT+GGG 0.519362 8:+16384204 None:intergenic
CGTAAATACCTTAATCAAGC+CGG 0.519512 8:+16384958 None:intergenic
GTTCGAGATCAGTTAAACAA+AGG 0.524433 8:-16385016 MsG0880042944.01.T01:CDS
CCACCTTAGGAAGAATAGAA+TGG 0.525280 8:-16383546 MsG0880042944.01.T01:CDS
TGAACAACAAGAACAAGAAG+AGG 0.528324 8:-16386522 MsG0880042944.01.T01:CDS
TTGCATTGCATCACTTGCTC+CGG 0.531461 8:+16384375 None:intergenic
AGAAGAGCAGCTTCTGTGGA+AGG 0.535601 8:+16384315 None:intergenic
TGTAACTTGCAGTGTGGAGA+GGG 0.539058 8:-16386318 MsG0880042944.01.T01:CDS
CTACAAGAGTACCTAGTGCC+AGG 0.543064 8:-16386178 MsG0880042944.01.T01:intron
CCATTCTATTCTTCCTAAGG+TGG 0.545441 8:+16383546 None:intergenic
TGAAAGACAATTGGATTGGG+TGG 0.553722 8:+16386868 None:intergenic
GCCATTGTAAATACTAGGGA+AGG 0.559174 8:-16383290 MsG0880042944.01.T01:CDS
GCGCAAGTTATGCAGGCACC+CGG 0.570203 8:-16383354 MsG0880042944.01.T01:CDS
GAAGATATGACAGCGAACAT+TGG 0.571920 8:-16383397 MsG0880042944.01.T01:intron
GTCACCATGTGAACAAATTG+AGG 0.575603 8:-16383620 MsG0880042944.01.T01:CDS
TCTTGCCATTGTAAATACTA+GGG 0.575822 8:-16383294 MsG0880042944.01.T01:CDS
AGGTGAGGTAAAACACAAAG+AGG 0.576387 8:+16386715 None:intergenic
AGAAGCTGCTCTTCTTGACT+GGG 0.576970 8:-16384307 MsG0880042944.01.T01:CDS
GCATCCTCAATTTGTTCACA+TGG 0.578905 8:+16383616 None:intergenic
CATTGGTCACGATTGAGAAA+CGG 0.582047 8:-16383380 MsG0880042944.01.T01:CDS
ACCTCAGGCTGAAACTCAGA+AGG 0.584588 8:+16386203 None:intergenic
AATGGAGCGTAGAGAAACTT+CGG 0.589113 8:-16383528 MsG0880042944.01.T01:CDS
GTAAATACTAGGGAAGGAAG+AGG 0.593283 8:-16383284 MsG0880042944.01.T01:CDS
GACACGTGTCTCACTTCGGT+TGG 0.596127 8:+16386266 None:intergenic
AGAGAATCAAGTACACCAGT+TGG 0.600276 8:+16384402 None:intergenic
CTACCAAAAGAAAATTCTCA+AGG 0.605610 8:-16383573 MsG0880042944.01.T01:CDS
TTGTAACTTGCAGTGTGGAG+AGG 0.609827 8:-16386319 MsG0880042944.01.T01:CDS
TCTTTGTTGAAGTTGAAAGG+AGG 0.610113 8:+16383646 None:intergenic
AGTTGGAAGCTCTCTAAACC+AGG 0.611737 8:+16384419 None:intergenic
TGGTCTTAAATAAAGCTGAA+AGG 0.615351 8:+16386678 None:intergenic
TTTCGCGCCCAACATGACAC+AGG 0.615447 8:-16384211 MsG0880042944.01.T01:intron
TCCTTCCCTAGTATTTACAA+TGG 0.616815 8:+16383289 None:intergenic
GGCTATTCTCAGCAGTAATG+CGG 0.618302 8:+16385050 None:intergenic
AGTGTTCTTATGATGATAGA+GGG 0.624124 8:-16385253 MsG0880042944.01.T01:CDS
ATGTGACACGTGTCTCACTT+CGG 0.625234 8:+16386262 None:intergenic
AGGTAAAACACAAAGAGGGT+TGG 0.626972 8:+16386720 None:intergenic
CATGAATCAATGCAGTTAAG+GGG 0.629505 8:+16383794 None:intergenic
AAGATTGCTCTCACATAGCA+TGG 0.632661 8:-16383695 MsG0880042944.01.T01:CDS
GGTTGGCCAGCTAATATCCA+AGG 0.633161 8:+16386283 None:intergenic
CTTTAAATGATGCAGTGTGA+AGG 0.637209 8:-16385303 MsG0880042944.01.T01:intron
AGGAAATTCATCATTGCAGG+AGG 0.637863 8:+16383666 None:intergenic
TTTCCATGGATGAAGGCAGA+AGG 0.638432 8:-16385079 MsG0880042944.01.T01:CDS
AACAAAGGGATAGTGCCACA+TGG 0.638676 8:-16385001 MsG0880042944.01.T01:CDS
TGATGGTTACTTGTGCAACA+GGG 0.639773 8:+16386558 None:intergenic
GCTTGCTCAATTGAAATACC+GGG 0.650080 8:+16383336 None:intergenic
GGTGAGGTAAAACACAAAGA+GGG 0.651496 8:+16386716 None:intergenic
TGGAAGGAAGTAACTTGACA+AGG 0.651985 8:+16384331 None:intergenic
TTAACAAACCTGTGTCATGT+TGG 0.658298 8:+16384203 None:intergenic
AGAGAAACTTCGGAGCTCCG+AGG 0.659373 8:-16383518 MsG0880042944.01.T01:CDS
ATCTATTGTAACTTGCAGTG+TGG 0.662855 8:-16386324 MsG0880042944.01.T01:CDS
TGTACTTGATTCTCTCACAC+CGG 0.686660 8:-16384394 MsG0880042944.01.T01:CDS
CAATGAACATCGATTCCATG+TGG 0.724200 8:+16384986 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAGAAAAAAAAAATGAA+GGG + Chr8:16383483-16383502 None:intergenic 15.0%
!! AACATGAAATTTATGTAATT+TGG - Chr8:16384008-16384027 MsG0880042944.01.T01:intron 15.0%
!! ATAGATATAAAAAATTTACC+TGG + Chr8:16383977-16383996 None:intergenic 15.0%
!! TAACAAGAAAAAAAAAATGA+AGG + Chr8:16383484-16383503 None:intergenic 15.0%
!! TTATTGTACTTACAATAAAA+TGG - Chr8:16384416-16384435 MsG0880042944.01.T01:CDS 15.0%
!!! TAACTATAACTATTTTCTAA+AGG + Chr8:16386712-16386731 None:intergenic 15.0%
!!! TCAATTTTGTTTAGAATTTT+TGG - Chr8:16384126-16384145 MsG0880042944.01.T01:intron 15.0%
!!! TTTTTATCATTATCTATTTC+AGG - Chr8:16385692-16385711 MsG0880042944.01.T01:intron 15.0%
!! AAAATACAGATAATCAATCA+AGG + Chr8:16384772-16384791 None:intergenic 20.0%
!! AAACACTAAGTTTGATTAAT+AGG - Chr8:16385413-16385432 MsG0880042944.01.T01:intron 20.0%
!! AAATTACAGAACATACTTTA+AGG + Chr8:16384963-16384982 None:intergenic 20.0%
!! AAGTACAATAAAAGATTCAT+AGG + Chr8:16384407-16384426 None:intergenic 20.0%
!! AATGATAAAAAGATATGACA+AGG + Chr8:16385683-16385702 None:intergenic 20.0%
!! CATACTTTATCTAATTTCAA+GGG + Chr8:16384293-16384312 None:intergenic 20.0%
!! CATCTAAATTTCATATTTCA+TGG - Chr8:16386079-16386098 MsG0880042944.01.T01:intron 20.0%
!! GGTTGAAAAATAAAAAGATA+CGG + Chr8:16385529-16385548 None:intergenic 20.0%
!! TCATACTTTATCTAATTTCA+AGG + Chr8:16384294-16384313 None:intergenic 20.0%
!!! AAAAGCCTAAAATTTTCTTA+AGG + Chr8:16384324-16384343 None:intergenic 20.0%
!!! AAAGCCTAAAATTTTCTTAA+GGG + Chr8:16384323-16384342 None:intergenic 20.0%
!!! ATAGTTACATGTGAATTTTA+AGG - Chr8:16385019-16385038 MsG0880042944.01.T01:CDS 20.0%
!!! TATCTTTTTATTTTTCAACC+TGG - Chr8:16385529-16385548 MsG0880042944.01.T01:intron 20.0%
!!! TGTATTTTTCATTTCACTTT+TGG + Chr8:16386279-16386298 None:intergenic 20.0%
!!! TTCAGTCAAATGAATTTTAT+AGG + Chr8:16385206-16385225 None:intergenic 20.0%
! ACTAAGTTTGATTAATAGGA+AGG - Chr8:16385417-16385436 MsG0880042944.01.T01:intron 25.0%
! ACTTGAATGAAAATGTGATA+TGG + Chr8:16383382-16383401 None:intergenic 25.0%
! AGAAAAAAAAAATGAAGGGT+TGG + Chr8:16383479-16383498 None:intergenic 25.0%
! ATTATGAAAGACAATTGGAT+TGG + Chr8:16383273-16383292 None:intergenic 25.0%
! CTCATAATTTACTGATAAGT+GGG - Chr8:16385330-16385349 MsG0880042944.01.T01:intron 25.0%
! CTTACAATAAAATGGTAGAA+TGG - Chr8:16384424-16384443 MsG0880042944.01.T01:intron 25.0%
! GCTAATTGTACAAAATATCA+GGG + Chr8:16386145-16386164 None:intergenic 25.0%
! TAAATCCTGACTATTATGTA+TGG - Chr8:16384577-16384596 MsG0880042944.01.T01:intron 25.0%
! TAATAAGCTGAAAAATTCGA+TGG - Chr8:16386184-16386203 MsG0880042944.01.T01:CDS 25.0%
! TCGTATTTGTCAAATTCATT+TGG - Chr8:16384989-16385008 MsG0880042944.01.T01:CDS 25.0%
! TGCAAACTCAGATAATAAAT+TGG + Chr8:16386061-16386080 None:intergenic 25.0%
! TGCTAATTGTACAAAATATC+AGG + Chr8:16386146-16386165 None:intergenic 25.0%
! TTATGAAAGACAATTGGATT+GGG + Chr8:16383272-16383291 None:intergenic 25.0%
!! AAGCCTAAAATTTTCTTAAG+GGG + Chr8:16384322-16384341 None:intergenic 25.0%
!! ATGAAAATGTGATATGGTTT+TGG + Chr8:16383376-16383395 None:intergenic 25.0%
!! ATTCCCCTTAAGAAAATTTT+AGG - Chr8:16384316-16384335 MsG0880042944.01.T01:CDS 25.0%
!! GAAAGGATCTGAATATTTTA+AGG + Chr8:16383442-16383461 None:intergenic 25.0%
!! GATATTTTGTACAATTAGCA+TGG - Chr8:16386146-16386165 MsG0880042944.01.T01:intron 25.0%
!! TGCGTAACTTGTAAATTATT+TGG - Chr8:16385261-16385280 MsG0880042944.01.T01:CDS 25.0%
!! TTTAAAACCAGAAATGTTTC+TGG - Chr8:16386000-16386019 MsG0880042944.01.T01:intron 25.0%
!!! AATGCTTTATTTCTTCTTGT+AGG - Chr8:16384368-16384387 MsG0880042944.01.T01:CDS 25.0%
!!! ATATGGTTTTGGTTAAGTAA+AGG + Chr8:16383365-16383384 None:intergenic 25.0%
!!! GTTACTTTTGATAGATTCAA+TGG - Chr8:16383895-16383914 MsG0880042944.01.T01:intron 25.0%
!!! GTTGATTATTTTTCTCAAAC+TGG - Chr8:16385643-16385662 MsG0880042944.01.T01:intron 25.0%
AAGCTGATGTAGATATTAAC+AGG - Chr8:16384449-16384468 MsG0880042944.01.T01:intron 30.0%
AGTGTTCTTATGATGATAGA+GGG - Chr8:16384881-16384900 MsG0880042944.01.T01:intron 30.0%
ATCGATGTTCATTGTTTATC+CGG - Chr8:16385157-16385176 MsG0880042944.01.T01:intron 30.0%
CAAAAGCAATTGTATTCTGA+AGG - Chr8:16384934-16384953 MsG0880042944.01.T01:intron 30.0%
CTACCAAAAGAAAATTCTCA+AGG - Chr8:16386561-16386580 MsG0880042944.01.T01:CDS 30.0%
CTATATCCAATATTGCAAAC+TGG + Chr8:16383771-16383790 None:intergenic 30.0%
GCTCATAATTTACTGATAAG+TGG - Chr8:16385329-16385348 MsG0880042944.01.T01:intron 30.0%
GGGAAATTATGAAAGACAAT+TGG + Chr8:16383278-16383297 None:intergenic 30.0%
GTTTCAAGACAATTCACAAA+AGG - Chr8:16383724-16383743 MsG0880042944.01.T01:CDS 30.0%
TAAAAAATTTACCTGGCACT+AGG + Chr8:16383970-16383989 None:intergenic 30.0%
TAATCAACCAGAAACATTTC+TGG + Chr8:16386010-16386029 None:intergenic 30.0%
TAGAGAGAAAATGAAGAGTT+TGG + Chr8:16383307-16383326 None:intergenic 30.0%
TCAAAACCAGTTTGCAATAT+TGG - Chr8:16383762-16383781 MsG0880042944.01.T01:CDS 30.0%
TCTTGCCATTGTAAATACTA+GGG - Chr8:16386840-16386859 MsG0880042944.01.T01:five_prime_UTR 30.0%
TTAACATTTCCAAAGCCTTT+CGG - Chr8:16385233-16385252 MsG0880042944.01.T01:intron 30.0%
TTAGTATTTGTTTGTGTCTC+TGG - Chr8:16384188-16384207 MsG0880042944.01.T01:intron 30.0%
TTCATCTTTCTTCTTGTTGA+TGG + Chr8:16383596-16383615 None:intergenic 30.0%
TTCGAGATCAGTTAAACAAA+GGG - Chr8:16385119-16385138 MsG0880042944.01.T01:intron 30.0%
! AAACTAGTTTGTCAGATTTG+TGG - Chr8:16384729-16384748 MsG0880042944.01.T01:intron 30.0%
! AGTCCTTGAGAATTTTCTTT+TGG + Chr8:16386567-16386586 None:intergenic 30.0%
! CCAACGCATACAAAATTTTA+AGG + Chr8:16385304-16385323 None:intergenic 30.0%
! CCTTAAAATTTTGTATGCGT+TGG - Chr8:16385301-16385320 MsG0880042944.01.T01:intron 30.0%
! CTGTTCATTTTCAACAACAT+CGG + Chr8:16385868-16385887 None:intergenic 30.0%
! GATAGATTCAATGGTTTTCT+TGG - Chr8:16383904-16383923 MsG0880042944.01.T01:intron 30.0%
! GTTTTCCATACATAATAGTC+AGG + Chr8:16384585-16384604 None:intergenic 30.0%
! TGGTCTTAAATAAAGCTGAA+AGG + Chr8:16383459-16383478 None:intergenic 30.0%
!! TGCATGATTTTGATCGAAAA+AGG + Chr8:16383525-16383544 None:intergenic 30.0%
!! TTTTGGTTAAGTAAAGGTTG+TGG + Chr8:16383359-16383378 None:intergenic 30.0%
!!! GATCTGAATATTTTAAGGTG+AGG + Chr8:16383437-16383456 None:intergenic 30.0%
!!! TTCAGATCTTTTTCAAGTGA+TGG - Chr8:16386661-16386680 MsG0880042944.01.T01:five_prime_UTR 30.0%
AAATGAAGAGTTTGGTTGAG+AGG + Chr8:16383299-16383318 None:intergenic 35.0%
AAGTTACGCAAATAACCGAA+AGG + Chr8:16385251-16385270 None:intergenic 35.0%
AATGAAGAGTTTGGTTGAGA+GGG + Chr8:16383298-16383317 None:intergenic 35.0%
ACTTTCTTGTGAAATCCAGA+TGG - Chr8:16386313-16386332 MsG0880042944.01.T01:CDS 35.0%
ATCATTATCTATTTCAGGCC+TGG - Chr8:16385697-16385716 MsG0880042944.01.T01:intron 35.0%
CACACTGCATCATTTAAAGA+CGG + Chr8:16384830-16384849 None:intergenic 35.0%
CAGTGTTCTTATGATGATAG+AGG - Chr8:16384880-16384899 MsG0880042944.01.T01:intron 35.0%
CATGAATCAATGCAGTTAAG+GGG + Chr8:16386343-16386362 None:intergenic 35.0%
CCAACTCAAAATACAATGCA+AGG - Chr8:16384669-16384688 MsG0880042944.01.T01:intron 35.0%
CGTAAATACCTTAATCAAGC+CGG + Chr8:16385179-16385198 None:intergenic 35.0%
CTTTAAATGATGCAGTGTGA+AGG - Chr8:16384831-16384850 MsG0880042944.01.T01:intron 35.0%
GCATGAATCAATGCAGTTAA+GGG + Chr8:16386344-16386363 None:intergenic 35.0%
GTAAAGGTTGTGGTGAAAAA+TGG + Chr8:16383349-16383368 None:intergenic 35.0%
GTACAATTAGCATGGCATAT+CGG - Chr8:16386154-16386173 MsG0880042944.01.T01:intron 35.0%
GTCTAATTTCATGCTTTCCA+TGG - Chr8:16385041-16385060 MsG0880042944.01.T01:CDS 35.0%
GTCTTGCCATTGTAAATACT+AGG - Chr8:16386839-16386858 MsG0880042944.01.T01:five_prime_UTR 35.0%
GTGTTCCAACAATTCTTCTA+AGG - Chr8:16384908-16384927 MsG0880042944.01.T01:intron 35.0%
GTTCGAGATCAGTTAAACAA+AGG - Chr8:16385118-16385137 MsG0880042944.01.T01:intron 35.0%
TAACAAACCTGTGTCATGTT+GGG + Chr8:16385933-16385952 None:intergenic 35.0%
TATCTATTAGAACAGCAACC+AGG + Chr8:16385550-16385569 None:intergenic 35.0%
TCAAAATCATGCAACATAGC+TGG - Chr8:16383532-16383551 MsG0880042944.01.T01:CDS 35.0%
TCCTCTTTGTTGAAGTTGAA+AGG + Chr8:16386494-16386513 None:intergenic 35.0%
TCCTTCCCTAGTATTTACAA+TGG + Chr8:16386848-16386867 None:intergenic 35.0%
TCCTTTCAACTTCAACAAAG+AGG - Chr8:16386490-16386509 MsG0880042944.01.T01:CDS 35.0%
TCTTTGTTGAAGTTGAAAGG+AGG + Chr8:16386491-16386510 None:intergenic 35.0%
TGAACAACAAGAACAAGAAG+AGG - Chr8:16383612-16383631 MsG0880042944.01.T01:CDS 35.0%
TGCATCCTTAGAAGAATTGT+TGG + Chr8:16384916-16384935 None:intergenic 35.0%
TGCATGAATCAATGCAGTTA+AGG + Chr8:16386345-16386364 None:intergenic 35.0%
TTAACAAACCTGTGTCATGT+TGG + Chr8:16385934-16385953 None:intergenic 35.0%
TTGTGGTGAAAAATGGAAGA+AGG + Chr8:16383342-16383361 None:intergenic 35.0%
TTTCTTCCTTGGATATTAGC+TGG - Chr8:16383845-16383864 MsG0880042944.01.T01:intron 35.0%
! AAGCAATTGTATTCTGAAGG+AGG - Chr8:16384937-16384956 MsG0880042944.01.T01:intron 35.0%
! ATCTATTGTAACTTGCAGTG+TGG - Chr8:16383810-16383829 MsG0880042944.01.T01:intron 35.0%
! CAATGATGATGATGATGATG+AGG - Chr8:16383675-16383694 MsG0880042944.01.T01:CDS 35.0%
! TTGTTTATCCGGCTTGATTA+AGG - Chr8:16385168-16385187 MsG0880042944.01.T01:intron 35.0%
!! CCTTGCATTGTATTTTGAGT+TGG + Chr8:16384672-16384691 None:intergenic 35.0%
!!! AATTTTGTATGCGTTGGCTA+TGG - Chr8:16385307-16385326 MsG0880042944.01.T01:intron 35.0%
!!! CTGGTTTTGATGATTGTTGT+TGG + Chr8:16383752-16383771 None:intergenic 35.0%
AAAGGACCACTTTCGTATCT+TGG + Chr8:16386694-16386713 None:intergenic 40.0%
AAGATTGCTCTCACATAGCA+TGG - Chr8:16386439-16386458 MsG0880042944.01.T01:CDS 40.0%
AATGGAGCGTAGAGAAACTT+CGG - Chr8:16386606-16386625 MsG0880042944.01.T01:CDS 40.0%
ACATGTTAATCTGCGTGGTA+AGG - Chr8:16384521-16384540 MsG0880042944.01.T01:intron 40.0%
AGAGAATCAAGTACACCAGT+TGG + Chr8:16385735-16385754 None:intergenic 40.0%
AGATGTTTGATCACGGACTT+CGG + Chr8:16385484-16385503 None:intergenic 40.0%
AGGAAATTCATCATTGCAGG+AGG + Chr8:16386471-16386490 None:intergenic 40.0%
AGGAGGAAATTCATCATTGC+AGG + Chr8:16386474-16386493 None:intergenic 40.0%
AGGTAAAACACAAAGAGGGT+TGG + Chr8:16383417-16383436 None:intergenic 40.0%
AGGTGAGGTAAAACACAAAG+AGG + Chr8:16383422-16383441 None:intergenic 40.0%
CATTGGTCACGATTGAGAAA+CGG - Chr8:16386754-16386773 MsG0880042944.01.T01:five_prime_UTR 40.0%
CCACCTTAGGAAGAATAGAA+TGG - Chr8:16386588-16386607 MsG0880042944.01.T01:CDS 40.0%
CCATTCTATTCTTCCTAAGG+TGG + Chr8:16386591-16386610 None:intergenic 40.0%
GAAGATATGACAGCGAACAT+TGG - Chr8:16386737-16386756 MsG0880042944.01.T01:five_prime_UTR 40.0%
GCCATTGTAAATACTAGGGA+AGG - Chr8:16386844-16386863 MsG0880042944.01.T01:five_prime_UTR 40.0%
GCTCCATTCTATTCTTCCTA+AGG + Chr8:16386594-16386613 None:intergenic 40.0%
GCTTGCTCAATTGAAATACC+GGG + Chr8:16386801-16386820 None:intergenic 40.0%
GGTGAGGTAAAACACAAAGA+GGG + Chr8:16383421-16383440 None:intergenic 40.0%
GTAAATACTAGGGAAGGAAG+AGG - Chr8:16386850-16386869 MsG0880042944.01.T01:five_prime_UTR 40.0%
GTAAATGAGCGAAACGAGAT+TGG + Chr8:16386039-16386058 None:intergenic 40.0%
GTAACTCCAAGATACGAAAG+TGG - Chr8:16386685-16386704 MsG0880042944.01.T01:five_prime_UTR 40.0%
GTCACCATGTGAACAAATTG+AGG - Chr8:16386514-16386533 MsG0880042944.01.T01:CDS 40.0%
GTCTCTGGTATAGAAGATTC+TGG - Chr8:16384203-16384222 MsG0880042944.01.T01:intron 40.0%
TGACTGGGCAATCAATTTGA+TGG - Chr8:16385842-16385861 MsG0880042944.01.T01:intron 40.0%
TGATGGTTACTTGTGCAACA+GGG + Chr8:16383579-16383598 None:intergenic 40.0%
TGGAAGGAAGTAACTTGACA+AGG + Chr8:16385806-16385825 None:intergenic 40.0%
TGGTGAAAAATGGAAGAAGG+AGG + Chr8:16383339-16383358 None:intergenic 40.0%
TGTACTTGATTCTCTCACAC+CGG - Chr8:16385740-16385759 MsG0880042944.01.T01:intron 40.0%
TTCATGCTTTCCATGGATGA+AGG - Chr8:16385048-16385067 MsG0880042944.01.T01:CDS 40.0%
TTTACAATGGCAAGACTTGC+AGG + Chr8:16386835-16386854 None:intergenic 40.0%
TTTCGACATGTTAATCTGCG+TGG - Chr8:16384516-16384535 MsG0880042944.01.T01:intron 40.0%
! GCATCCTCAATTTGTTCACA+TGG + Chr8:16386521-16386540 None:intergenic 40.0%
! GGCTTGCTCAATTGAAATAC+CGG + Chr8:16386802-16386821 None:intergenic 40.0%
! TTGATGGTTACTTGTGCAAC+AGG + Chr8:16383580-16383599 None:intergenic 40.0%
!! CAATGAACATCGATTCCATG+TGG + Chr8:16385151-16385170 None:intergenic 40.0%
!! TGATGCAGTGTGAAGGTATT+TGG - Chr8:16384838-16384857 MsG0880042944.01.T01:intron 40.0%
AACAAAGGGATAGTGCCACA+TGG - Chr8:16385133-16385152 MsG0880042944.01.T01:intron 45.0%
AATCTGCGTGGTAAGGAACT+CGG - Chr8:16384528-16384547 MsG0880042944.01.T01:intron 45.0%
AGAAGCTGCTCTTCTTGACT+GGG - Chr8:16385827-16385846 MsG0880042944.01.T01:intron 45.0%
AGACTTGCAGGCTTCTTAGA+TGG + Chr8:16386823-16386842 None:intergenic 45.0%
AGGAAAAAGGAATCGAGACG+AGG - Chr8:16386636-16386655 MsG0880042944.01.T01:exon 45.0%
AGTTGGAAGCTCTCTAAACC+AGG + Chr8:16385718-16385737 None:intergenic 45.0%
ATGTGACACGTGTCTCACTT+CGG + Chr8:16383875-16383894 None:intergenic 45.0%
ATTCCTTCTGCCTTCATCCA+TGG + Chr8:16385061-16385080 None:intergenic 45.0%
CGCAAATAACCGAAAGGCTT+TGG + Chr8:16385245-16385264 None:intergenic 45.0%
GACTTCGGCCATTTATGCAT+AGG + Chr8:16385469-16385488 None:intergenic 45.0%
GAGCACGAGATGTTTGATCA+CGG + Chr8:16385491-16385510 None:intergenic 45.0%
GAGGGAAGATGTTTCTTCCT+TGG - Chr8:16383834-16383853 MsG0880042944.01.T01:intron 45.0%
GATCTCGAACGAATGCTTCT+TGG + Chr8:16385108-16385127 None:intergenic 45.0%
GCTGGAGACCTATGCATAAA+TGG - Chr8:16385458-16385477 MsG0880042944.01.T01:intron 45.0%
GGCTATTCTCAGCAGTAATG+CGG + Chr8:16385087-16385106 None:intergenic 45.0%
TGGTTTAGAGAGCTTCCAAC+TGG - Chr8:16385717-16385736 MsG0880042944.01.T01:intron 45.0%
TTCTCAAGGACTTCCACCTT+AGG - Chr8:16386575-16386594 MsG0880042944.01.T01:CDS 45.0%
TTGCATTGCATCACTTGCTC+CGG + Chr8:16385762-16385781 None:intergenic 45.0%
TTTCCATGGATGAAGGCAGA+AGG - Chr8:16385055-16385074 MsG0880042944.01.T01:CDS 45.0%
! AACCTCTCACCACTAGCTTA+AGG + Chr8:16384557-16384576 None:intergenic 45.0%
! CGTCTCGATTCCTTTTTCCT+CGG + Chr8:16386636-16386655 None:intergenic 45.0%
! TGTAACTTGCAGTGTGGAGA+GGG - Chr8:16383816-16383835 MsG0880042944.01.T01:intron 45.0%
! TTGTAACTTGCAGTGTGGAG+AGG - Chr8:16383815-16383834 MsG0880042944.01.T01:intron 45.0%
AAGTGGGCAGTTTGTCTGAG+CGG - Chr8:16385346-16385365 MsG0880042944.01.T01:intron 50.0%
ACCTCAGGCTGAAACTCAGA+AGG + Chr8:16383934-16383953 None:intergenic 50.0%
ACTCGGACACCTTAAGCTAG+TGG - Chr8:16384545-16384564 MsG0880042944.01.T01:intron 50.0%
AGAAGAGCAGCTTCTGTGGA+AGG + Chr8:16385822-16385841 None:intergenic 50.0%
ATAGGTCTCCAGCTGTAGAG+TGG + Chr8:16385451-16385470 None:intergenic 50.0%
CACCTTAAGCTAGTGGTGAG+AGG - Chr8:16384552-16384571 MsG0880042944.01.T01:intron 50.0%
CAGAAGCTGCTCTTCTTGAC+TGG - Chr8:16385826-16385845 MsG0880042944.01.T01:intron 50.0%
CTGGCACTAGGTACTCTTGT+AGG + Chr8:16383958-16383977 None:intergenic 50.0%
GGAAGGAAGAGGAGAAGCTT+GGG - Chr8:16386861-16386880 MsG0880042944.01.T01:five_prime_UTR 50.0%
GGTACTCTTGTAGGAACCTC+AGG + Chr8:16383949-16383968 None:intergenic 50.0%
GTAGTTCAGACGCTGCTAAG+CGG - Chr8:16384615-16384634 MsG0880042944.01.T01:intron 50.0%
GTCAAGAAGAGCAGCTTCTG+TGG + Chr8:16385826-16385845 None:intergenic 50.0%
TACTGCATCCACTCTACAGC+TGG - Chr8:16385440-16385459 MsG0880042944.01.T01:intron 50.0%
! GGTTGGCCAGCTAATATCCA+AGG + Chr8:16383854-16383873 None:intergenic 50.0%
!! CTACAAGAGTACCTAGTGCC+AGG - Chr8:16383956-16383975 MsG0880042944.01.T01:intron 50.0%
AGAGAAACTTCGGAGCTCCG+AGG - Chr8:16386616-16386635 MsG0880042944.01.T01:CDS 55.0%
AGTGGGCAGTTTGTCTGAGC+GGG - Chr8:16385347-16385366 MsG0880042944.01.T01:intron 55.0%
CAGTTAAGGGGTCTGCCATC+TGG + Chr8:16386331-16386350 None:intergenic 55.0%
CTTCGGAGCTCCGAGGAAAA+AGG - Chr8:16386623-16386642 MsG0880042944.01.T01:CDS 55.0%
GAATGCGCGCAACGTAGCTA+TGG - Chr8:16385899-16385918 MsG0880042944.01.T01:intron 55.0%
GACACGTGTCTCACTTCGGT+TGG + Chr8:16383871-16383890 None:intergenic 55.0%
GCCTTCTGAGTTTCAGCCTG+AGG - Chr8:16383930-16383949 MsG0880042944.01.T01:intron 55.0%
GGGAAGGAAGAGGAGAAGCT+TGG - Chr8:16386860-16386879 MsG0880042944.01.T01:five_prime_UTR 55.0%
TTTCGCGCCCAACATGACAC+AGG - Chr8:16385923-16385942 MsG0880042944.01.T01:intron 55.0%
ACGGCGTGCGCAAGTTATGC+AGG - Chr8:16386773-16386792 MsG0880042944.01.T01:five_prime_UTR 60.0%
GAGCGGGGAAGGTTGCTCAA+GGG - Chr8:16385363-16385382 MsG0880042944.01.T01:intron 60.0%
GCAGTTTGTCTGAGCGGGGA+AGG - Chr8:16385352-16385371 MsG0880042944.01.T01:intron 60.0%
GCGCAAGTTATGCAGGCACC+CGG - Chr8:16386780-16386799 MsG0880042944.01.T01:five_prime_UTR 60.0%
GTGGGCAGTTTGTCTGAGCG+GGG - Chr8:16385348-16385367 MsG0880042944.01.T01:intron 60.0%
TGAGCGGGGAAGGTTGCTCA+AGG - Chr8:16385362-16385381 MsG0880042944.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 16383268 16386888 16383268 ID=MsG0880042944.01;Name=MsG0880042944.01
Chr8 mRNA 16383268 16386888 16383268 ID=MsG0880042944.01.T01;Parent=MsG0880042944.01;Name=MsG0880042944.01.T01;_AED=0.31;_eAED=0.32;_QI=246|0.6|0.33|1|1|1|6|0|456
Chr8 exon 16383268 16383406 16383268 ID=MsG0880042944.01.T01:exon:4319;Parent=MsG0880042944.01.T01
Chr8 exon 16383499 16383824 16383499 ID=MsG0880042944.01.T01:exon:4318;Parent=MsG0880042944.01.T01
Chr8 exon 16384212 16384442 16384212 ID=MsG0880042944.01.T01:exon:4317;Parent=MsG0880042944.01.T01
Chr8 exon 16384967 16385115 16384967 ID=MsG0880042944.01.T01:exon:4316;Parent=MsG0880042944.01.T01
Chr8 exon 16385249 16385310 16385249 ID=MsG0880042944.01.T01:exon:4315;Parent=MsG0880042944.01.T01
Chr8 exon 16386179 16386888 16386179 ID=MsG0880042944.01.T01:exon:4314;Parent=MsG0880042944.01.T01
Chr8 five_prime_UTR 16386643 16386888 16386643 ID=MsG0880042944.01.T01:five_prime_utr;Parent=MsG0880042944.01.T01
Chr8 CDS 16386179 16386642 16386179 ID=MsG0880042944.01.T01:cds;Parent=MsG0880042944.01.T01
Chr8 CDS 16385249 16385310 16385249 ID=MsG0880042944.01.T01:cds;Parent=MsG0880042944.01.T01
Chr8 CDS 16384967 16385115 16384967 ID=MsG0880042944.01.T01:cds;Parent=MsG0880042944.01.T01
Chr8 CDS 16384212 16384442 16384212 ID=MsG0880042944.01.T01:cds;Parent=MsG0880042944.01.T01
Chr8 CDS 16383499 16383824 16383499 ID=MsG0880042944.01.T01:cds;Parent=MsG0880042944.01.T01
Chr8 CDS 16383268 16383406 16383268 ID=MsG0880042944.01.T01:cds;Parent=MsG0880042944.01.T01
Gene Sequence

>MsG0880042944.01.T01

ATGACTCGCCTTTTTCGATCAAAATCATGCAACATAGCTGGTTTCAGTGTGTCTGAATTCAACCCTGTTGCACAAGTAACCATCAACAAGAAGAAAGATGAACAACAAGAACAAGAAGAGGAAGAAGAAGAAGAAGAAGAACTAGAAAGTGATGACAATGACAATGATGATGATGATGATGAGGAATATGAGAAATATTCAAGCAATAATGTTTCAAGACAATTCACAAAAGGATCCAACAACAATCATCAAAACCAGTTTGCAATATTGGATATAGTTGTTGCTGCTCTTAAGAAATCTATTGTAACTTGCAGTGTGGAGAGGGAAGATGTTTCTTCCTTGGATATTAGCTGGCCAACCGAAGTGAGACACGTGTCACATGTTACTTTTGATAGATTCAATGGTTTTCTTGGTTTGCCTTCTGAGTTTCAGCCTGAGGTTCCTACAAGAGTACCTAGTGCCAGTGTGAAGGTATTTGGAGTTTCTGCAAAGTCAATGCAGTGTTCTTATGATGATAGAGGGAACAGTCTAATTTCATGCTTTCCATGGATGAAGGCAGAAGGAATATTCCGCATTACTGCTGAGAATAGCCAAGAAGCATTCGTTCGAGATCAGTTAAACAAAGGGATAGTGCCACATGGAATCGATGTTCATTGTTTATCCGGCTTGATTAAGGCCTGGTTTAGAGAGCTTCCAACTGGTGTACTTGATTCTCTCACACCGGAGCAAGTGATGCAATGCAACACAGAAGATGATTGTACTAACCTTGTCAAGTTACTTCCTTCCACAGAAGCTGCTCTTCTTGACTGGGCAATCAATTTGATGGCCGATGTTGTTGAAAATGAACAGTTCAATAAGATGAATGCGCGCAACGTAGCTATGGTTTTCGCGCCCAACATGACACAGATGGCAGACCCCTTAACTGCATTGATTCATGCAGTACAAGTTATGAACTTCTTAAAGACATTGATTTTGAAGACGCTTCGAGAAAGAGAAGAATCGATTGATAATGCAAGATTGCTCTCACATAGCATGGACTTTGCCTCCTGCAATGATGAATTTCCTCCTTTCAACTTCAACAAAGAGGAGTCACCATGTGAACAAATTGAGGATGCTTGTGACAAAAACTCATCAACTACCAAAAGAAAATTCTCAAGGACTTCCACCTTAGGAAGAATAGAATGGAGCGTAGAGAAACTTCGGAGCTCCGAGGAAAAAGGAATCGAGACGAGCGAACATTGGTCACGATTGAGAAACGGCGTGCGCAAGTTATGCAGGCACCCGGTATTTCAATTGAGCAAGCCATCTAAGAAGCCTGCAAGTCTTGCCATTGTAAATACTAGGGAAGGAAGAGGAGAAGCTTGGGCTTGA
Protein sequence

>MsG0880042944.01.T01

MTRLFRSKSCNIAGFSVSEFNPVAQVTINKKKDEQQEQEEEEEEEEELESDDNDNDDDDDEEYEKYSSNNVSRQFTKGSNNNHQNQFAILDIVVAALKKSIVTCSVEREDVSSLDISWPTEVRHVSHVTFDRFNGFLGLPSEFQPEVPTRVPSASVKVFGVSAKSMQCSYDDRGNSLISCFPWMKAEGIFRITAENSQEAFVRDQLNKGIVPHGIDVHCLSGLIKAWFRELPTGVLDSLTPEQVMQCNTEDDCTNLVKLLPSTEAALLDWAINLMADVVENEQFNKMNARNVAMVFAPNMTQMADPLTALIHAVQVMNFLKTLILKTLREREESIDNARLLSHSMDFASCNDEFPPFNFNKEESPCEQIEDACDKNSSTTKRKFSRTSTLGRIEWSVEKLRSSEEKGIETSEHWSRLRNGVRKLCRHPVFQLSKPSKKPASLAIVNTREGRGEAWA*