AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022094.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022094.01.T01 MTR_4g082350 98.883 358 4 0 1 358 1 358 0.0 723
MsG0480022094.01.T01 MTR_4g109020 28.082 292 198 9 49 331 6 294 7.48e-29 114
MsG0480022094.01.T01 MTR_3g037110 27.703 296 186 8 49 330 6 287 6.56e-26 106
MsG0480022094.01.T01 MTR_4g096890 26.073 303 197 7 41 336 7 289 1.82e-17 82.4
MsG0480022094.01.T01 MTR_7g021620 26.316 304 199 7 39 336 5 289 8.59e-17 80.5
MsG0480022094.01.T01 MTR_3g088975 24.756 307 211 6 39 336 5 300 2.08e-15 76.6
MsG0480022094.01.T01 MTR_1g029440 23.127 307 225 6 35 336 1 301 1.06e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022094.01.T01 AT1G69430 66.462 325 102 2 17 335 8 331 2.94e-154 437
MsG0480022094.01.T01 AT1G26650 63.939 330 116 3 24 350 2 331 8.36e-154 436
MsG0480022094.01.T01 AT4G19950 27.451 306 190 9 41 336 7 290 8.80e-15 74.7
MsG0480022094.01.T01 AT5G61340 28.311 219 136 8 119 329 80 285 1.58e-12 68.2
MsG0480022094.01.T01 AT5G61340 28.311 219 136 8 119 329 113 318 1.60e-12 68.6

Find 66 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGTTCTGGAGGATGTTTC+GGG 0.171105 4:-68118238 MsG0480022094.01.T01:CDS
CTCGGTTCTGGAGGATGTTT+CGG 0.203669 4:-68118239 MsG0480022094.01.T01:CDS
GAGAAACTATAAGGATTCTT+AGG 0.245365 4:-68118807 MsG0480022094.01.T01:CDS
TGTAGTGCATTGGCTGTTCT+TGG 0.255096 4:-68118364 MsG0480022094.01.T01:CDS
TGCATTGGTGATCTCGGTTC+TGG 0.279598 4:-68118251 MsG0480022094.01.T01:CDS
TTGTGAAGAATCTTACTATT+AGG 0.290531 4:-68118693 MsG0480022094.01.T01:CDS
GAGGTTGTTCTCCTAGGATT+AGG 0.293873 4:-68118945 MsG0480022094.01.T01:CDS
TATTCGAGAAAGAAGTTTGA+TGG 0.334246 4:-68118505 MsG0480022094.01.T01:CDS
TTTACGTCAAGCTTTAGAAT+TGG 0.356836 4:-68118884 MsG0480022094.01.T01:CDS
ATATTCTTAGAGAAACTATA+AGG 0.357854 4:-68118816 MsG0480022094.01.T01:CDS
GCCCATTGTTTCAGCAGAAA+TGG 0.362209 4:+68117916 None:intergenic
AATTCAATCAATGAATGCTT+TGG 0.379030 4:-68118839 MsG0480022094.01.T01:CDS
ATATATCTTGGATCAACAAT+AGG 0.400163 4:-68118154 MsG0480022094.01.T01:CDS
TGTTGTGTATGCTGCATTGA+TGG 0.401523 4:-68118326 MsG0480022094.01.T01:CDS
AGGTTGTTCTCCTAGGATTA+GGG 0.404867 4:-68118944 MsG0480022094.01.T01:CDS
CAGCGTTGCATTGGTGATCT+CGG 0.410427 4:-68118257 MsG0480022094.01.T01:CDS
GTGTGATCGTGGCAAAGTTT+TGG 0.412812 4:-68118471 MsG0480022094.01.T01:CDS
GTCGGTCTGCTAATATATCT+TGG 0.415716 4:-68118166 MsG0480022094.01.T01:CDS
GCAGCATACACAACAACATT+AGG 0.417117 4:+68118334 None:intergenic
GGCCATTTCTGCTGAAACAA+TGG 0.429412 4:-68117918 MsG0480022094.01.T01:CDS
AACAATAGGAATGGCCTTTG+TGG 0.433278 4:-68118140 MsG0480022094.01.T01:CDS
GACAAAAGCACAGCAGAAAC+AGG 0.438148 4:+68118739 None:intergenic
TGCGGATACGGTGATTGCTT+CGG 0.443552 4:-68118602 MsG0480022094.01.T01:CDS
TGGGCTTGTACAGTTATTGT+TGG 0.445242 4:-68118424 MsG0480022094.01.T01:CDS
ATTGTTGTTATTGTCGAAAA+CGG 0.446504 4:-68118554 MsG0480022094.01.T01:CDS
CAAGTGGAGGTTGTTCTCCT+AGG 0.449328 4:-68118951 MsG0480022094.01.T01:CDS
GTAGTGCATTGGCTGTTCTT+GGG 0.473904 4:-68118363 MsG0480022094.01.T01:CDS
GCCATTTCTGCTGAAACAAT+GGG 0.478130 4:-68117917 MsG0480022094.01.T01:CDS
AGATACTTGCGACGTATATG+TGG 0.480298 4:-68118444 MsG0480022094.01.T01:CDS
TTGTCAGAGATTTGCGGATA+CGG 0.482242 4:-68118614 MsG0480022094.01.T01:CDS
AATCATTTGCAGCGTTGCAT+TGG 0.483424 4:-68118266 MsG0480022094.01.T01:CDS
TGACAAGATTGCTTAATCAT+AGG 0.488189 4:+68118631 None:intergenic
GGATTCTTAGGTTTAATGCA+TGG 0.498954 4:-68118795 MsG0480022094.01.T01:CDS
TCTTACTATTAGGCTTATGT+TGG 0.501948 4:-68118683 MsG0480022094.01.T01:CDS
TTTAGCATGGAGAACTCAAA+TGG 0.507639 4:-68117968 MsG0480022094.01.T01:CDS
GCAATCTTGTCAGAGATTTG+CGG 0.510716 4:-68118620 MsG0480022094.01.T01:CDS
ATATGATCTACCCTAATCCT+AGG 0.527505 4:+68118934 None:intergenic
AATGTGGGAAGGGCCGCTCT+TGG 0.532814 4:-68118062 MsG0480022094.01.T01:CDS
GATTCTTAGGTTTAATGCAT+GGG 0.536767 4:-68118794 MsG0480022094.01.T01:CDS
ATAGGAATGGCCTTTGTGGA+GGG 0.546492 4:-68118136 MsG0480022094.01.T01:CDS
TGCTTAATCATAGGTCTCAA+AGG 0.555452 4:+68118640 None:intergenic
GTTATTGTCGAAAACGGCTG+TGG 0.558964 4:-68118548 MsG0480022094.01.T01:CDS
GTGTATGCTGCATTGATGGT+CGG 0.566529 4:-68118322 MsG0480022094.01.T01:CDS
GTGATGGATCTTCGAGAATG+TGG 0.574992 4:-68118078 MsG0480022094.01.T01:CDS
AATAGGAATGGCCTTTGTGG+AGG 0.586579 4:-68118137 MsG0480022094.01.T01:CDS
TCTTGGATCAACAATAGGAA+TGG 0.587680 4:-68118149 MsG0480022094.01.T01:CDS
TTTAATCAAAGGTCAGACTC+AGG 0.592291 4:-68118188 MsG0480022094.01.T01:CDS
AGAGTAAAGACACTGAGTTA+TGG 0.598968 4:-68118100 MsG0480022094.01.T01:CDS
TAGGAATGGCCTTTGTGGAG+GGG 0.600285 4:-68118135 MsG0480022094.01.T01:CDS
ATCAAAGGTCAGACTCAGGT+CGG 0.608137 4:-68118184 MsG0480022094.01.T01:CDS
CGGTTCTGGAGGATGTTTCG+GGG 0.610462 4:-68118237 MsG0480022094.01.T01:CDS
AAAATGGAGATTTCAAGTGG+AGG 0.612604 4:-68118964 MsG0480022094.01.T01:CDS
ATGGAGAACTCAAATGGCGA+TGG 0.614239 4:-68117962 MsG0480022094.01.T01:CDS
ATGTTGGTTGCTGAAACTAG+TGG 0.620640 4:-68118667 MsG0480022094.01.T01:CDS
TATAGATTCTATGATGAGTG+CGG 0.621002 4:-68118014 MsG0480022094.01.T01:CDS
ATTGGTGATCTCGGTTCTGG+AGG 0.630178 4:-68118248 MsG0480022094.01.T01:CDS
ATGCACTACAAAACGCAACA+AGG 0.631744 4:+68118377 None:intergenic
GATACTTGCGACGTATATGT+GGG 0.634555 4:-68118443 MsG0480022094.01.T01:CDS
AACAACAATGTAGCATACAA+AGG 0.636060 4:+68118568 None:intergenic
AAGACACTGAGTTATGGTGA+TGG 0.640856 4:-68118094 MsG0480022094.01.T01:CDS
GATCTTCGAGAATGTGGGAA+GGG 0.641099 4:-68118072 MsG0480022094.01.T01:CDS
GGATCTTCGAGAATGTGGGA+AGG 0.642671 4:-68118073 MsG0480022094.01.T01:CDS
CTCAAACAACCCCTCCACAA+AGG 0.646189 4:+68118126 None:intergenic
GAATACATTATCACCAAGAG+CGG 0.651587 4:+68118049 None:intergenic
GGTTCTGGAGGATGTTTCGG+GGG 0.680880 4:-68118236 MsG0480022094.01.T01:CDS
TGATGGATCTTCGAGAATGT+GGG 0.681312 4:-68118077 MsG0480022094.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCTTAGAGAAACTATA+AGG - Chr4:68118052-68118071 MsG0480022094.01.T01:CDS 20.0%
! AATTCAATCAATGAATGCTT+TGG - Chr4:68118029-68118048 MsG0480022094.01.T01:CDS 25.0%
! ATATATCTTGGATCAACAAT+AGG - Chr4:68118714-68118733 MsG0480022094.01.T01:CDS 25.0%
! GTAGATCATATGAAAAAAGA+GGG - Chr4:68117946-68117965 MsG0480022094.01.T01:CDS 25.0%
! TTGTGAAGAATCTTACTATT+AGG - Chr4:68118175-68118194 MsG0480022094.01.T01:CDS 25.0%
!! ATTGTTGTTATTGTCGAAAA+CGG - Chr4:68118314-68118333 MsG0480022094.01.T01:CDS 25.0%
!!! CAACTCAATTTTAGAAACTA+TGG - Chr4:68118929-68118948 MsG0480022094.01.T01:CDS 25.0%
AACAACAATGTAGCATACAA+AGG + Chr4:68118303-68118322 None:intergenic 30.0%
GAGAAACTATAAGGATTCTT+AGG - Chr4:68118061-68118080 MsG0480022094.01.T01:CDS 30.0%
GATTCTTAGGTTTAATGCAT+GGG - Chr4:68118074-68118093 MsG0480022094.01.T01:CDS 30.0%
GGTAGATCATATGAAAAAAG+AGG - Chr4:68117945-68117964 MsG0480022094.01.T01:CDS 30.0%
TATAGATTCTATGATGAGTG+CGG - Chr4:68118854-68118873 MsG0480022094.01.T01:CDS 30.0%
TATTCGAGAAAGAAGTTTGA+TGG - Chr4:68118363-68118382 MsG0480022094.01.T01:CDS 30.0%
TCTTACTATTAGGCTTATGT+TGG - Chr4:68118185-68118204 MsG0480022094.01.T01:CDS 30.0%
TGACAAGATTGCTTAATCAT+AGG + Chr4:68118240-68118259 None:intergenic 30.0%
! TGTGCTTTTGTCTAATGTAT+TGG - Chr4:68118140-68118159 MsG0480022094.01.T01:CDS 30.0%
!! GGTGATAATGTATTCTTTTG+TGG - Chr4:68118827-68118846 MsG0480022094.01.T01:CDS 30.0%
!! TTTACGTCAAGCTTTAGAAT+TGG - Chr4:68117984-68118003 MsG0480022094.01.T01:CDS 30.0%
!!! AGGTCGAGTATTTTAATCAA+AGG - Chr4:68118669-68118688 MsG0480022094.01.T01:CDS 30.0%
AGAGTAAAGACACTGAGTTA+TGG - Chr4:68118768-68118787 MsG0480022094.01.T01:CDS 35.0%
ATATGATCTACCCTAATCCT+AGG + Chr4:68117937-68117956 None:intergenic 35.0%
GAATACATTATCACCAAGAG+CGG + Chr4:68118822-68118841 None:intergenic 35.0%
GGATTCTTAGGTTTAATGCA+TGG - Chr4:68118073-68118092 MsG0480022094.01.T01:CDS 35.0%
TCTTGGATCAACAATAGGAA+TGG - Chr4:68118719-68118738 MsG0480022094.01.T01:CDS 35.0%
TGCTTAATCATAGGTCTCAA+AGG + Chr4:68118231-68118250 None:intergenic 35.0%
TTTAATCAAAGGTCAGACTC+AGG - Chr4:68118680-68118699 MsG0480022094.01.T01:CDS 35.0%
TTTAGCATGGAGAACTCAAA+TGG - Chr4:68118900-68118919 MsG0480022094.01.T01:CDS 35.0%
! CAGTTGTAGATCTTTTAGCA+TGG - Chr4:68118887-68118906 MsG0480022094.01.T01:CDS 35.0%
AACAATAGGAATGGCCTTTG+TGG - Chr4:68118728-68118747 MsG0480022094.01.T01:CDS 40.0%
AAGACACTGAGTTATGGTGA+TGG - Chr4:68118774-68118793 MsG0480022094.01.T01:CDS 40.0%
AATCATTTGCAGCGTTGCAT+TGG - Chr4:68118602-68118621 MsG0480022094.01.T01:CDS 40.0%
AGATACTTGCGACGTATATG+TGG - Chr4:68118424-68118443 MsG0480022094.01.T01:CDS 40.0%
AGGTTGTTCTCCTAGGATTA+GGG - Chr4:68117924-68117943 MsG0480022094.01.T01:CDS 40.0%
ATGCACTACAAAACGCAACA+AGG + Chr4:68118494-68118513 None:intergenic 40.0%
GATACTTGCGACGTATATGT+GGG - Chr4:68118425-68118444 MsG0480022094.01.T01:CDS 40.0%
GCAATCTTGTCAGAGATTTG+CGG - Chr4:68118248-68118267 MsG0480022094.01.T01:CDS 40.0%
GCAGCATACACAACAACATT+AGG + Chr4:68118537-68118556 None:intergenic 40.0%
GCCATTTCTGCTGAAACAAT+GGG - Chr4:68118951-68118970 MsG0480022094.01.T01:CDS 40.0%
GTCGGTCTGCTAATATATCT+TGG - Chr4:68118702-68118721 MsG0480022094.01.T01:CDS 40.0%
TGGGCTTGTACAGTTATTGT+TGG - Chr4:68118444-68118463 MsG0480022094.01.T01:CDS 40.0%
TGTTGTGTATGCTGCATTGA+TGG - Chr4:68118542-68118561 MsG0480022094.01.T01:CDS 40.0%
TTGTCAGAGATTTGCGGATA+CGG - Chr4:68118254-68118273 MsG0480022094.01.T01:CDS 40.0%
! ATGTTGGTTGCTGAAACTAG+TGG - Chr4:68118201-68118220 MsG0480022094.01.T01:CDS 40.0%
! TGATGGATCTTCGAGAATGT+GGG - Chr4:68118791-68118810 MsG0480022094.01.T01:CDS 40.0%
! TGTTGCGTTTTGTAGTGCAT+TGG - Chr4:68118494-68118513 MsG0480022094.01.T01:CDS 40.0%
!! TTCGAAGTTTTGTGTGATCG+TGG - Chr4:68118386-68118405 MsG0480022094.01.T01:CDS 40.0%
AATAGGAATGGCCTTTGTGG+AGG - Chr4:68118731-68118750 MsG0480022094.01.T01:CDS 45.0%
ATAGGAATGGCCTTTGTGGA+GGG - Chr4:68118732-68118751 MsG0480022094.01.T01:CDS 45.0%
ATCAAAGGTCAGACTCAGGT+CGG - Chr4:68118684-68118703 MsG0480022094.01.T01:CDS 45.0%
ATGGAGAACTCAAATGGCGA+TGG - Chr4:68118906-68118925 MsG0480022094.01.T01:CDS 45.0%
GAGGTTGTTCTCCTAGGATT+AGG - Chr4:68117923-68117942 MsG0480022094.01.T01:CDS 45.0%
GATCTTCGAGAATGTGGGAA+GGG - Chr4:68118796-68118815 MsG0480022094.01.T01:CDS 45.0%
GCCCATTGTTTCAGCAGAAA+TGG + Chr4:68118955-68118974 None:intergenic 45.0%
GGCCATTTCTGCTGAAACAA+TGG - Chr4:68118950-68118969 MsG0480022094.01.T01:CDS 45.0%
GTTATTGTCGAAAACGGCTG+TGG - Chr4:68118320-68118339 MsG0480022094.01.T01:CDS 45.0%
! GACAAAAGCACAGCAGAAAC+AGG + Chr4:68118132-68118151 None:intergenic 45.0%
! GTGTATGCTGCATTGATGGT+CGG - Chr4:68118546-68118565 MsG0480022094.01.T01:CDS 45.0%
! GTGTGATCGTGGCAAAGTTT+TGG - Chr4:68118397-68118416 MsG0480022094.01.T01:CDS 45.0%
!! GTAGTGCATTGGCTGTTCTT+GGG - Chr4:68118505-68118524 MsG0480022094.01.T01:CDS 45.0%
!! GTGATGGATCTTCGAGAATG+TGG - Chr4:68118790-68118809 MsG0480022094.01.T01:CDS 45.0%
!! TGATCGTGGCAAAGTTTTGG+AGG - Chr4:68118400-68118419 MsG0480022094.01.T01:CDS 45.0%
!! TGTAGTGCATTGGCTGTTCT+TGG - Chr4:68118504-68118523 MsG0480022094.01.T01:CDS 45.0%
CAAGTGGAGGTTGTTCTCCT+AGG - Chr4:68117917-68117936 MsG0480022094.01.T01:CDS 50.0%
CTCAAACAACCCCTCCACAA+AGG + Chr4:68118745-68118764 None:intergenic 50.0%
GGATCTTCGAGAATGTGGGA+AGG - Chr4:68118795-68118814 MsG0480022094.01.T01:CDS 50.0%
TAGGAATGGCCTTTGTGGAG+GGG - Chr4:68118733-68118752 MsG0480022094.01.T01:CDS 50.0%
TGCGGATACGGTGATTGCTT+CGG - Chr4:68118266-68118285 MsG0480022094.01.T01:CDS 50.0%
! ATTGGTGATCTCGGTTCTGG+AGG - Chr4:68118620-68118639 MsG0480022094.01.T01:CDS 50.0%
! CAGCGTTGCATTGGTGATCT+CGG - Chr4:68118611-68118630 MsG0480022094.01.T01:CDS 50.0%
! TGCATTGGTGATCTCGGTTC+TGG - Chr4:68118617-68118636 MsG0480022094.01.T01:CDS 50.0%
!! CTCGGTTCTGGAGGATGTTT+CGG - Chr4:68118629-68118648 MsG0480022094.01.T01:CDS 50.0%
!! TCGGTTCTGGAGGATGTTTC+GGG - Chr4:68118630-68118649 MsG0480022094.01.T01:CDS 50.0%
!! CGGTTCTGGAGGATGTTTCG+GGG - Chr4:68118631-68118650 MsG0480022094.01.T01:CDS 55.0%
!! GGTTCTGGAGGATGTTTCGG+GGG - Chr4:68118632-68118651 MsG0480022094.01.T01:CDS 55.0%
AATGTGGGAAGGGCCGCTCT+TGG - Chr4:68118806-68118825 MsG0480022094.01.T01:CDS 60.0%
! GGAGGATGTTTCGGGGGCGC+AGG - Chr4:68118638-68118657 MsG0480022094.01.T01:CDS 70.0%
! CGGGGGCGCAGGCGATGCTA+AGG - Chr4:68118649-68118668 MsG0480022094.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 68117907 68118983 68117907 ID=MsG0480022094.01;Name=MsG0480022094.01
Chr4 mRNA 68117907 68118983 68117907 ID=MsG0480022094.01.T01;Parent=MsG0480022094.01;Name=MsG0480022094.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|358
Chr4 exon 68117907 68118983 68117907 ID=MsG0480022094.01.T01:exon:8464;Parent=MsG0480022094.01.T01
Chr4 CDS 68117907 68118983 68117907 ID=MsG0480022094.01.T01:cds;Parent=MsG0480022094.01.T01
Gene Sequence

>MsG0480022094.01.T01

ATGGAGATTTCAAGTGGAGGTTGTTCTCCTAGGATTAGGGTAGATCATATGAAAAAAGAGGGATCAGATGATACACGTTTACGTCAAGCTTTAGAATTGGAACTTTCTGATCATAATCATGAAATTCAATCAATGAATGCTTTGGATATTCTTAGAGAAACTATAAGGATTCTTAGGTTTAATGCATGGGCTTTTATGATAATCGCTTTTTTGCTTATTTGTCCTGTTTCTGCTGTGCTTTTGTCTAATGTATTGGTTGATGAATCTATTGTGAAGAATCTTACTATTAGGCTTATGTTGGTTGCTGAAACTAGTGGTCTTCCTTTGAGACCTATGATTAAGCAATCTTGTCAGAGATTTGCGGATACGGTGATTGCTTCGGCGATGTGTTTTCCTTTGTATGCTACATTGTTGTTATTGTCGAAAACGGCTGTGGTTTATTCTGTTGATTGTACTTATTCGAGAAAGAAGTTTGATGGTTCGAAGTTTTGTGTGATCGTGGCAAAGTTTTGGAGGAAGATACTTGCGACGTATATGTGGGCTTGTACAGTTATTGTTGGTTGCATCACTATGTTTTGTGTTTTCCTTGTTGCGTTTTGTAGTGCATTGGCTGTTCTTGGGTTTTCTCCTAATGTTGTTGTGTATGCTGCATTGATGGTCGGATTAGTTTTCTCTGTTGTATTTGCGAATGCGATAATCATTTGCAGCGTTGCATTGGTGATCTCGGTTCTGGAGGATGTTTCGGGGGCGCAGGCGATGCTAAGGTCGAGTATTTTAATCAAAGGTCAGACTCAGGTCGGTCTGCTAATATATCTTGGATCAACAATAGGAATGGCCTTTGTGGAGGGGTTGTTTGAGCACAGAGTAAAGACACTGAGTTATGGTGATGGATCTTCGAGAATGTGGGAAGGGCCGCTCTTGGTGATAATGTATTCTTTTGTGGTGCTTATAGATTCTATGATGAGTGCGGTTTTCTATTTCAGTTGTAGATCTTTTAGCATGGAGAACTCAAATGGCGATGGCAACTCAATTTTAGAAACTATGGCCATTTCTGCTGAAACAATGGGCATTCAATGA

Protein sequence

>MsG0480022094.01.T01

MEISSGGCSPRIRVDHMKKEGSDDTRLRQALELELSDHNHEIQSMNALDILRETIRILRFNAWAFMIIAFLLICPVSAVLLSNVLVDESIVKNLTIRLMLVAETSGLPLRPMIKQSCQRFADTVIASAMCFPLYATLLLLSKTAVVYSVDCTYSRKKFDGSKFCVIVAKFWRKILATYMWACTVIVGCITMFCVFLVAFCSALAVLGFSPNVVVYAALMVGLVFSVVFANAIIICSVALVISVLEDVSGAQAMLRSSILIKGQTQVGLLIYLGSTIGMAFVEGLFEHRVKTLSYGDGSSRMWEGPLLVIMYSFVVLIDSMMSAVFYFSCRSFSMENSNGDGNSILETMAISAETMGIQ*