Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045544.01.T01 | XP_003629148.1 | 98.421 | 190 | 2 | 1 | 1 | 189 | 1 | 190 | 2.61E-129 | 371 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045544.01.T01 | G7L9F6 | 98.421 | 190 | 2 | 1 | 1 | 189 | 1 | 190 | 1.24e-129 | 371 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000151.01 | MsG0880045544.01 | 0.826262 | 2.987148e-54 | 2.787380e-51 |
| MsG0880045544.01 | MsG0880047285.01 | -0.810196 | 1.299665e-50 | 7.784276e-48 |
| MsG0180004390.01 | MsG0880045544.01 | 0.802569 | 5.271157e-49 | 2.590650e-46 |
| MsG0780040794.01 | MsG0880045544.01 | 0.841100 | 5.831948e-58 | 8.478614e-55 |
| MsG0880042202.01 | MsG0880045544.01 | 0.823269 | 1.517108e-53 | 1.300011e-50 |
| MsG0880042948.01 | MsG0880045544.01 | 0.806406 | 8.353771e-50 | 4.530414e-47 |
| MsG0280011073.01 | MsG0880045544.01 | 0.812649 | 3.812551e-51 | 2.437551e-48 |
| MsG0280011330.01 | MsG0880045544.01 | 0.829120 | 6.147787e-55 | 6.233599e-52 |
| MsG0280011376.01 | MsG0880045544.01 | 0.814381 | 1.586365e-51 | 1.062833e-48 |
| MsG0380012005.01 | MsG0880045544.01 | 0.801319 | 9.523279e-49 | 4.534367e-46 |
| MsG0380012184.01 | MsG0880045544.01 | 0.804052 | 2.598941e-49 | 1.326846e-46 |
| MsG0380012331.01 | MsG0880045544.01 | 0.822410 | 2.404500e-53 | 2.011079e-50 |
| MsG0380014727.01 | MsG0880045544.01 | 0.825860 | 3.722599e-54 | 3.434426e-51 |
| MsG0380014866.01 | MsG0880045544.01 | 0.800034 | 1.741454e-48 | 8.025882e-46 |
| MsG0680032516.01 | MsG0880045544.01 | 0.824739 | 6.855181e-54 | 6.125644e-51 |
| MsG0680033136.01 | MsG0880045544.01 | 0.819470 | 1.142340e-52 | 8.798831e-50 |
| MsG0680035618.01 | MsG0880045544.01 | 0.823198 | 1.575849e-53 | 1.347693e-50 |
| MsG0480018519.01 | MsG0880045544.01 | 0.801589 | 8.386169e-49 | 4.020034e-46 |
| MsG0480018606.01 | MsG0880045544.01 | 0.838753 | 2.384931e-57 | 3.225062e-54 |
| MsG0480022535.01 | MsG0880045544.01 | 0.848982 | 4.331168e-60 | 8.090385e-57 |
| MsG0480023251.01 | MsG0880045544.01 | 0.805908 | 1.063527e-49 | 5.695081e-47 |
| MsG0580024491.01 | MsG0880045544.01 | -0.848392 | 6.311754e-60 | 1.156584e-56 |
| MsG0580024596.01 | MsG0880045544.01 | 0.825219 | 5.280967e-54 | 4.783864e-51 |
| MsG0380015215.01 | MsG0880045544.01 | -0.835827 | 1.337330e-56 | 1.654538e-53 |
| MsG0380015374.01 | MsG0880045544.01 | 0.801046 | 1.083261e-48 | 5.122658e-46 |
| MsG0380015745.01 | MsG0880045544.01 | 0.816435 | 5.539645e-52 | 3.924747e-49 |
| MsG0380015810.01 | MsG0880045544.01 | 0.842563 | 2.395916e-58 | 3.647125e-55 |
| MsG0380016740.01 | MsG0880045544.01 | 0.849187 | 3.798116e-60 | 7.143449e-57 |
| MsG0380017322.01 | MsG0880045544.01 | 0.815319 | 9.824666e-52 | 6.751522e-49 |
| MsG0580027923.01 | MsG0880045544.01 | 0.818999 | 1.461607e-52 | 1.111291e-49 |
| MsG0280009157.01 | MsG0880045544.01 | 0.805315 | 1.416278e-49 | 7.469260e-47 |
| MsG0780036181.01 | MsG0880045544.01 | 0.803721 | 3.046009e-49 | 1.541810e-46 |
| MsG0780038833.01 | MsG0880045544.01 | 0.801436 | 9.011795e-49 | 4.303487e-46 |
| MsG0780039218.01 | MsG0880045544.01 | 0.843050 | 1.778247e-58 | 2.747984e-55 |
| MsG0780039438.01 | MsG0880045544.01 | 0.812270 | 4.612489e-51 | 2.919616e-48 |
| MsG0780039711.01 | MsG0880045544.01 | 0.810806 | 9.596738e-51 | 5.841950e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045544.01.T01 | MTR_8g073850 | 98.421 | 190 | 2 | 1 | 1 | 189 | 1 | 190 | 3.16e-133 | 371 |
| MsG0880045544.01.T01 | MTR_8g073770 | 97.368 | 190 | 4 | 1 | 1 | 189 | 1 | 190 | 1.95e-131 | 366 |
| MsG0880045544.01.T01 | MTR_4g013320 | 33.784 | 148 | 83 | 7 | 41 | 180 | 55 | 195 | 1.62e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045544.01.T01 | AT1G58170 | 30.065 | 153 | 98 | 3 | 33 | 180 | 33 | 181 | 2.73e-11 | 60.1 |
Find 34 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTGAGTTTCATATTTAT+TGG | 0.205961 | 8:+60820089 | MsG0880045544.01.T01:CDS |
| ATTATAGTGAATGGTATAAA+AGG | 0.253437 | 8:+60819734 | MsG0880045544.01.T01:CDS |
| TTCTTCTCCAGTTGATCTTA+AGG | 0.286848 | 8:+60820048 | MsG0880045544.01.T01:CDS |
| TTCAGCAATTCCAACTAGTT+TGG | 0.300270 | 8:-60819843 | None:intergenic |
| TCTTCTCCAGTTGATCTTAA+GGG | 0.323970 | 8:+60820049 | MsG0880045544.01.T01:CDS |
| AAGGGTTCTTTATCTATTGT+TGG | 0.359486 | 8:+60819944 | MsG0880045544.01.T01:CDS |
| ACACAAACAACAACACCTTT+TGG | 0.363090 | 8:+60819770 | MsG0880045544.01.T01:CDS |
| TCCATCACCTCTAGTCTTGA+TGG | 0.373011 | 8:+60819872 | MsG0880045544.01.T01:CDS |
| GAAGATTTCATGTTTGTTCA+AGG | 0.381719 | 8:+60820016 | MsG0880045544.01.T01:CDS |
| ATGTTAGAGTCAAAGGATCT+TGG | 0.408571 | 8:-60819808 | None:intergenic |
| TCTTCGTTTGAAGAATCATA+AGG | 0.412755 | 8:+60819925 | MsG0880045544.01.T01:CDS |
| GTAAGACCCTTAAGATCAAC+TGG | 0.414315 | 8:-60820055 | None:intergenic |
| CTTCGTTTGAAGAATCATAA+GGG | 0.426758 | 8:+60819926 | MsG0880045544.01.T01:CDS |
| TATGATGATTGAATTGCATA+TGG | 0.429737 | 8:-60820115 | None:intergenic |
| TCTTCAGTACCTCCAACTAT+AGG | 0.437094 | 8:-60819998 | None:intergenic |
| AATGGTATAAAAGGAAATGC+TGG | 0.442818 | 8:+60819743 | MsG0880045544.01.T01:CDS |
| AACAAACAATGTTAAAGCTT+CGG | 0.449018 | 8:+60819970 | MsG0880045544.01.T01:CDS |
| AGCAATTCCAACTAGTTTGG+AGG | 0.471386 | 8:-60819840 | None:intergenic |
| CATGAGACAATAAACAAAAC+AGG | 0.474989 | 8:+60819707 | MsG0880045544.01.T01:CDS |
| AATAGATCCTCCAAACTAGT+TGG | 0.477342 | 8:+60819833 | MsG0880045544.01.T01:CDS |
| ACAGGTTATATTATAGTGAA+TGG | 0.479522 | 8:+60819725 | MsG0880045544.01.T01:CDS |
| GGTTCTTTATCTATTGTTGG+TGG | 0.486963 | 8:+60819947 | MsG0880045544.01.T01:CDS |
| GCTTCGGATCATCCTATAGT+TGG | 0.488687 | 8:+60819986 | MsG0880045544.01.T01:CDS |
| CTATTTGATGTTAGAGTCAA+AGG | 0.493391 | 8:-60819815 | None:intergenic |
| CCATCACCTCTAGTCTTGAT+GGG | 0.495648 | 8:+60819873 | MsG0880045544.01.T01:CDS |
| TATGCAATTCAATCATCATA+AGG | 0.505454 | 8:+60820118 | MsG0880045544.01.T01:CDS |
| AACACAAATAATGTTCCAAA+AGG | 0.513629 | 8:-60819785 | None:intergenic |
| TTGGTAAAGTGAAAAGTGAA+TGG | 0.522971 | 8:-60819684 | None:intergenic |
| CCCATCAAGACTAGAGGTGA+TGG | 0.541174 | 8:-60819873 | None:intergenic |
| TCGGATCATCCTATAGTTGG+AGG | 0.552506 | 8:+60819989 | MsG0880045544.01.T01:CDS |
| ATGATGAGAAGAGCTAGAAG+TGG | 0.572468 | 8:-60819636 | None:intergenic |
| AAACTAGTTGGAATTGCTGA+AGG | 0.580758 | 8:+60819845 | MsG0880045544.01.T01:CDS |
| TCGAAGCCCATCAAGACTAG+AGG | 0.650725 | 8:-60819879 | None:intergenic |
| GATGATTGAATTGCATATGG+AGG | 0.659805 | 8:-60820112 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATTGAGTTTCATATTTAT+TGG | + | Chr8:60820089-60820108 | MsG0880045544.01.T01:CDS | 20.0% |
| !! | ATTATAGTGAATGGTATAAA+AGG | + | Chr8:60819734-60819753 | MsG0880045544.01.T01:CDS | 20.0% |
| !!! | AGTGGTTTTAAATTGAATTA+TGG | - | Chr8:60819621-60819640 | None:intergenic | 20.0% |
| ! | AACAAACAATGTTAAAGCTT+CGG | + | Chr8:60819970-60819989 | MsG0880045544.01.T01:CDS | 25.0% |
| ! | AACACAAATAATGTTCCAAA+AGG | - | Chr8:60819788-60819807 | None:intergenic | 25.0% |
| ! | ACAGGTTATATTATAGTGAA+TGG | + | Chr8:60819725-60819744 | MsG0880045544.01.T01:CDS | 25.0% |
| ! | TATGATGATTGAATTGCATA+TGG | - | Chr8:60820118-60820137 | None:intergenic | 25.0% |
| !!! | TTTTGTTTATTGTCTCATGT+TGG | - | Chr8:60819706-60819725 | None:intergenic | 25.0% |
| AAGGGTTCTTTATCTATTGT+TGG | + | Chr8:60819944-60819963 | MsG0880045544.01.T01:CDS | 30.0% | |
| AATGGTATAAAAGGAAATGC+TGG | + | Chr8:60819743-60819762 | MsG0880045544.01.T01:CDS | 30.0% | |
| CATGAGACAATAAACAAAAC+AGG | + | Chr8:60819707-60819726 | MsG0880045544.01.T01:CDS | 30.0% | |
| CTATTTGATGTTAGAGTCAA+AGG | - | Chr8:60819818-60819837 | None:intergenic | 30.0% | |
| CTTCGTTTGAAGAATCATAA+GGG | + | Chr8:60819926-60819945 | MsG0880045544.01.T01:CDS | 30.0% | |
| GAAGATTTCATGTTTGTTCA+AGG | + | Chr8:60820016-60820035 | MsG0880045544.01.T01:CDS | 30.0% | |
| TCTTCGTTTGAAGAATCATA+AGG | + | Chr8:60819925-60819944 | MsG0880045544.01.T01:CDS | 30.0% | |
| TTGGTAAAGTGAAAAGTGAA+TGG | - | Chr8:60819687-60819706 | None:intergenic | 30.0% | |
| !! | GTGAAAAGTGAATGGATTTT+AGG | - | Chr8:60819679-60819698 | None:intergenic | 30.0% |
| AAACTAGTTGGAATTGCTGA+AGG | + | Chr8:60819845-60819864 | MsG0880045544.01.T01:CDS | 35.0% | |
| AATAGATCCTCCAAACTAGT+TGG | + | Chr8:60819833-60819852 | MsG0880045544.01.T01:CDS | 35.0% | |
| ATGTTAGAGTCAAAGGATCT+TGG | - | Chr8:60819811-60819830 | None:intergenic | 35.0% | |
| GATGATTGAATTGCATATGG+AGG | - | Chr8:60820115-60820134 | None:intergenic | 35.0% | |
| GGTTCTTTATCTATTGTTGG+TGG | + | Chr8:60819947-60819966 | MsG0880045544.01.T01:CDS | 35.0% | |
| TCTTCTCCAGTTGATCTTAA+GGG | + | Chr8:60820049-60820068 | MsG0880045544.01.T01:CDS | 35.0% | |
| TTCAGCAATTCCAACTAGTT+TGG | - | Chr8:60819846-60819865 | None:intergenic | 35.0% | |
| TTCTTCTCCAGTTGATCTTA+AGG | + | Chr8:60820048-60820067 | MsG0880045544.01.T01:CDS | 35.0% | |
| ! | ACACAAACAACAACACCTTT+TGG | + | Chr8:60819770-60819789 | MsG0880045544.01.T01:CDS | 35.0% |
| !! | ATTTTAGGTGATTGTGGTGA+TGG | - | Chr8:60819664-60819683 | None:intergenic | 35.0% |
| !! | GAATGGATTTTAGGTGATTG+TGG | - | Chr8:60819670-60819689 | None:intergenic | 35.0% |
| ATGATGAGAAGAGCTAGAAG+TGG | - | Chr8:60819639-60819658 | None:intergenic | 40.0% | |
| GTAAGACCCTTAAGATCAAC+TGG | - | Chr8:60820058-60820077 | None:intergenic | 40.0% | |
| TCTTCAGTACCTCCAACTAT+AGG | - | Chr8:60820001-60820020 | None:intergenic | 40.0% | |
| ! | AGCAATTCCAACTAGTTTGG+AGG | - | Chr8:60819843-60819862 | None:intergenic | 40.0% |
| GCTTCGGATCATCCTATAGT+TGG | + | Chr8:60819986-60820005 | MsG0880045544.01.T01:CDS | 45.0% | |
| TCGGATCATCCTATAGTTGG+AGG | + | Chr8:60819989-60820008 | MsG0880045544.01.T01:CDS | 45.0% | |
| !! | CCATCACCTCTAGTCTTGAT+GGG | + | Chr8:60819873-60819892 | MsG0880045544.01.T01:CDS | 45.0% |
| !! | TCCATCACCTCTAGTCTTGA+TGG | + | Chr8:60819872-60819891 | MsG0880045544.01.T01:CDS | 45.0% |
| CCCATCAAGACTAGAGGTGA+TGG | - | Chr8:60819876-60819895 | None:intergenic | 50.0% | |
| TCGAAGCCCATCAAGACTAG+AGG | - | Chr8:60819882-60819901 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 60819569 | 60820138 | 60819569 | ID=MsG0880045544.01;Name=MsG0880045544.01 |
| Chr8 | mRNA | 60819569 | 60820138 | 60819569 | ID=MsG0880045544.01.T01;Parent=MsG0880045544.01;Name=MsG0880045544.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|189 |
| Chr8 | exon | 60819569 | 60820138 | 60819569 | ID=MsG0880045544.01.T01:exon:12533;Parent=MsG0880045544.01.T01 |
| Chr8 | CDS | 60819569 | 60820138 | 60819569 | ID=MsG0880045544.01.T01:cds;Parent=MsG0880045544.01.T01 |
| Gene Sequence |
| Protein sequence |
