AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011330.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011330.01.T01 MTR_2g102140 98.936 282 2 1 1 281 30 311 0.0 572
MsG0280011330.01.T01 MTR_4g012060 46.996 283 121 6 2 281 34 290 3.74e-68 213
MsG0280011330.01.T01 MTR_1g072110 38.462 143 63 5 108 243 110 234 1.68e-18 82.8
MsG0280011330.01.T01 MTR_7g095150 47.887 71 35 1 173 243 132 200 2.79e-15 73.2
MsG0280011330.01.T01 MTR_1g111640 28.340 247 95 9 1 240 27 198 1.50e-12 66.2
MsG0280011330.01.T01 MTR_1g103640 42.188 64 34 1 178 241 117 177 3.20e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011330.01.T01 AT1G79960 36.879 282 127 9 6 270 41 288 7.12e-43 148
MsG0280011330.01.T01 AT5G04820 35.338 133 62 4 109 241 102 210 2.24e-14 71.6
MsG0280011330.01.T01 AT4G14860 43.284 67 31 2 179 243 109 170 1.39e-11 62.4

Find 46 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAGGTTACCATAGAATT+TGG 0.233067 2:+82499852 MsG0280011330.01.T01:CDS
CGAGTCCTTACGATGATTTC+AGG 0.250240 2:+82499577 MsG0280011330.01.T01:CDS
GAAAATTGATTGGGACTTCA+TGG 0.309098 2:+82499656 MsG0280011330.01.T01:CDS
CAGGAACCACCAATACGTTT+TGG 0.387081 2:-82499288 None:intergenic
TTTGTTGTTATTGTCGTTAA+CGG 0.389175 2:-82499115 None:intergenic
ACAATCAAAGGAAAATTGAT+TGG 0.389239 2:+82499646 MsG0280011330.01.T01:CDS
CTAAGAGGAACCAAATTCTA+TGG 0.390296 2:-82499862 None:intergenic
CAGGCGATCAATGCAAGAAT+TGG 0.391866 2:+82499596 MsG0280011330.01.T01:CDS
ATTGTCGTTAACGGCGTTAG+AGG 0.410961 2:-82499106 None:intergenic
TATTGGTGGTTCCTGGTTGT+TGG 0.439269 2:+82499296 MsG0280011330.01.T01:CDS
TTAGGATTGGTAGGGAAGTC+AGG 0.440786 2:+82499817 MsG0280011330.01.T01:CDS
CAAAACGTATTGGTGGTTCC+TGG 0.454127 2:+82499289 MsG0280011330.01.T01:CDS
TTGATCTCATCACTGTTATG+AGG 0.467638 2:+82499748 MsG0280011330.01.T01:CDS
GAATTGGTGGAATCAAAGTA+TGG 0.467801 2:+82499612 MsG0280011330.01.T01:CDS
GAAGAATTCAGAGGCTGCTC+CGG 0.469764 2:+82499770 MsG0280011330.01.T01:CDS
CGATTAACCAAAATGCTCTC+TGG 0.470779 2:+82499027 None:intergenic
CGTGTAGCGTGAGAACTGTT+AGG 0.472831 2:+82499799 MsG0280011330.01.T01:CDS
AGGTAACTCAGGATCATTGA+TGG 0.483746 2:+82499386 MsG0280011330.01.T01:CDS
ACACCACCTCAAGATCTTTG+TGG 0.490451 2:+82499339 MsG0280011330.01.T01:CDS
CAATCAAAGGAAAATTGATT+GGG 0.491479 2:+82499647 MsG0280011330.01.T01:CDS
TGAAATTCTCAAAGAGAAAA+CGG 0.493044 2:-82499170 None:intergenic
ATCAATGATCCTGAGTTACC+TGG 0.499280 2:-82499384 None:intergenic
AACAGCATCACCTTCAAAAG+AGG 0.511692 2:+82499479 MsG0280011330.01.T01:CDS
TGAGAACTGTTAGGATTGGT+AGG 0.512241 2:+82499808 MsG0280011330.01.T01:CDS
GCGATCAATGCAAGAATTGG+TGG 0.527393 2:+82499599 MsG0280011330.01.T01:CDS
CTGAAATCATCGTAAGGACT+CGG 0.533629 2:-82499576 None:intergenic
AAATTCTATGGTAACCTGCT+TGG 0.538786 2:-82499850 None:intergenic
CTCACGCTACACGGCTTCGC+CGG 0.538841 2:-82499789 None:intergenic
GAGGAACCACCAAAACGTAT+TGG 0.549662 2:+82499279 MsG0280011330.01.T01:CDS
GCACGATTCTTCGTGAAACC+AGG 0.558059 2:+82499366 MsG0280011330.01.T01:CDS
TATGTTTCTGCCGTTATCCA+AGG 0.558329 2:-82499079 None:intergenic
CTAACAGTTCTCACGCTACA+CGG 0.576486 2:-82499798 None:intergenic
TTCGTGAAACCAGGTAACTC+AGG 0.588008 2:+82499375 MsG0280011330.01.T01:CDS
AGCGTGAGAACTGTTAGGAT+TGG 0.588361 2:+82499804 MsG0280011330.01.T01:CDS
CAGGAAGAAGAAGACCAAGC+AGG 0.606262 2:+82499836 MsG0280011330.01.T01:CDS
TCTCGGTGACTCCAACAACC+AGG 0.608261 2:-82499307 None:intergenic
GTAAGATTGAAAACAATCAA+AGG 0.608557 2:+82499634 MsG0280011330.01.T01:CDS
GAACCACCAAAACGTATTGG+TGG 0.614019 2:+82499282 MsG0280011330.01.T01:CDS
GCTGAGCCACAAAGATCTTG+AGG 0.618030 2:-82499345 None:intergenic
GATCGCCTGAAATCATCGTA+AGG 0.625029 2:-82499582 None:intergenic
TGTGATGATTGTGATCATCA+TGG 0.625055 2:-82499515 None:intergenic
GAGAACTGTTAGGATTGGTA+GGG 0.631326 2:+82499809 MsG0280011330.01.T01:CDS
GGTGGTGTTGTAATGAATCT+CGG 0.636568 2:-82499324 None:intergenic
AATTGATTGGGACTTCATGG+AGG 0.677814 2:+82499659 MsG0280011330.01.T01:CDS
TGTTATGAGGAAGAATTCAG+AGG 0.682074 2:+82499761 MsG0280011330.01.T01:CDS
GAGCCACAAAGATCTTGAGG+TGG 0.753269 2:-82499342 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAATCAAAGGAAAATTGAT+TGG + Chr2:82499646-82499665 MsG0280011330.01.T01:CDS 25.0%
! CAACAAAAACAAAAGAATCT+CGG + Chr2:82499242-82499261 MsG0280011330.01.T01:CDS 25.0%
! CAATCAAAGGAAAATTGATT+GGG + Chr2:82499647-82499666 MsG0280011330.01.T01:CDS 25.0%
! GTAAGATTGAAAACAATCAA+AGG + Chr2:82499634-82499653 MsG0280011330.01.T01:CDS 25.0%
! TGAAATTCTCAAAGAGAAAA+CGG - Chr2:82499173-82499192 None:intergenic 25.0%
!!! TTTGTTGTTATTGTCGTTAA+CGG - Chr2:82499118-82499137 None:intergenic 25.0%
! AAGGAAAAAGATGGTGTTTT+GGG - Chr2:82499063-82499082 None:intergenic 30.0%
! ACTACAATAACCTCTTTTGA+AGG - Chr2:82499492-82499511 None:intergenic 30.0%
AAATTCTATGGTAACCTGCT+TGG - Chr2:82499853-82499872 None:intergenic 35.0%
AAGCAGGTTACCATAGAATT+TGG + Chr2:82499852-82499871 MsG0280011330.01.T01:CDS 35.0%
CTAAGAGGAACCAAATTCTA+TGG - Chr2:82499865-82499884 None:intergenic 35.0%
GAAAATTGATTGGGACTTCA+TGG + Chr2:82499656-82499675 MsG0280011330.01.T01:CDS 35.0%
GAATTGGTGGAATCAAAGTA+TGG + Chr2:82499612-82499631 MsG0280011330.01.T01:CDS 35.0%
TGTGATGATTGTGATCATCA+TGG - Chr2:82499518-82499537 None:intergenic 35.0%
TGTTATGAGGAAGAATTCAG+AGG + Chr2:82499761-82499780 MsG0280011330.01.T01:CDS 35.0%
TTGATCTCATCACTGTTATG+AGG + Chr2:82499748-82499767 MsG0280011330.01.T01:CDS 35.0%
! AACAGTGCAACACAATTTTC+AGG - Chr2:82499546-82499565 None:intergenic 35.0%
! CAAAACACCATCTTTTTCCT+TGG + Chr2:82499062-82499081 MsG0280011330.01.T01:CDS 35.0%
! CAAGGAAAAAGATGGTGTTT+TGG - Chr2:82499064-82499083 None:intergenic 35.0%
! CCATCTTTTTCCTTGGATAA+CGG + Chr2:82499069-82499088 MsG0280011330.01.T01:CDS 35.0%
AACAGCATCACCTTCAAAAG+AGG + Chr2:82499479-82499498 MsG0280011330.01.T01:CDS 40.0%
AATTGATTGGGACTTCATGG+AGG + Chr2:82499659-82499678 MsG0280011330.01.T01:CDS 40.0%
AGGTAACTCAGGATCATTGA+TGG + Chr2:82499386-82499405 MsG0280011330.01.T01:CDS 40.0%
ATCAATGATCCTGAGTTACC+TGG - Chr2:82499387-82499406 None:intergenic 40.0%
CCGTTATCCAAGGAAAAAGA+TGG - Chr2:82499072-82499091 None:intergenic 40.0%
CTCGGATGAAAAAAACAACG+AGG + Chr2:82499260-82499279 MsG0280011330.01.T01:CDS 40.0%
CTGAAATCATCGTAAGGACT+CGG - Chr2:82499579-82499598 None:intergenic 40.0%
! GAGAACTGTTAGGATTGGTA+GGG + Chr2:82499809-82499828 MsG0280011330.01.T01:CDS 40.0%
! TATGTTTCTGCCGTTATCCA+AGG - Chr2:82499082-82499101 None:intergenic 40.0%
! TGAGAACTGTTAGGATTGGT+AGG + Chr2:82499808-82499827 MsG0280011330.01.T01:CDS 40.0%
!! GGTGGTGTTGTAATGAATCT+CGG - Chr2:82499327-82499346 None:intergenic 40.0%
ACACCACCTCAAGATCTTTG+TGG + Chr2:82499339-82499358 MsG0280011330.01.T01:CDS 45.0%
AGCGTGAGAACTGTTAGGAT+TGG + Chr2:82499804-82499823 MsG0280011330.01.T01:CDS 45.0%
ATTGTCGTTAACGGCGTTAG+AGG - Chr2:82499109-82499128 None:intergenic 45.0%
CAGGCGATCAATGCAAGAAT+TGG + Chr2:82499596-82499615 MsG0280011330.01.T01:CDS 45.0%
CGAGTCCTTACGATGATTTC+AGG + Chr2:82499577-82499596 MsG0280011330.01.T01:CDS 45.0%
CTAACAGTTCTCACGCTACA+CGG - Chr2:82499801-82499820 None:intergenic 45.0%
GAACCACCAAAACGTATTGG+TGG + Chr2:82499282-82499301 MsG0280011330.01.T01:CDS 45.0%
GAGGAACCACCAAAACGTAT+TGG + Chr2:82499279-82499298 MsG0280011330.01.T01:CDS 45.0%
GATCGCCTGAAATCATCGTA+AGG - Chr2:82499585-82499604 None:intergenic 45.0%
GCGATCAATGCAAGAATTGG+TGG + Chr2:82499599-82499618 MsG0280011330.01.T01:CDS 45.0%
TTCGTGAAACCAGGTAACTC+AGG + Chr2:82499375-82499394 MsG0280011330.01.T01:CDS 45.0%
! CAAAACGTATTGGTGGTTCC+TGG + Chr2:82499289-82499308 MsG0280011330.01.T01:CDS 45.0%
! CAGGAACCACCAATACGTTT+TGG - Chr2:82499291-82499310 None:intergenic 45.0%
! GAACCACCAATACGTTTTGG+TGG - Chr2:82499288-82499307 None:intergenic 45.0%
! TATTGGTGGTTCCTGGTTGT+TGG + Chr2:82499296-82499315 MsG0280011330.01.T01:CDS 45.0%
! TTAGGATTGGTAGGGAAGTC+AGG + Chr2:82499817-82499836 MsG0280011330.01.T01:CDS 45.0%
CAGGAAGAAGAAGACCAAGC+AGG + Chr2:82499836-82499855 MsG0280011330.01.T01:CDS 50.0%
CGTGTAGCGTGAGAACTGTT+AGG + Chr2:82499799-82499818 MsG0280011330.01.T01:CDS 50.0%
GAAGAATTCAGAGGCTGCTC+CGG + Chr2:82499770-82499789 MsG0280011330.01.T01:CDS 50.0%
GAGCCACAAAGATCTTGAGG+TGG - Chr2:82499345-82499364 None:intergenic 50.0%
GCACGATTCTTCGTGAAACC+AGG + Chr2:82499366-82499385 MsG0280011330.01.T01:CDS 50.0%
GCTGAGCCACAAAGATCTTG+AGG - Chr2:82499348-82499367 None:intergenic 50.0%
TCTCGGTGACTCCAACAACC+AGG - Chr2:82499310-82499329 None:intergenic 55.0%
CTCACGCTACACGGCTTCGC+CGG - Chr2:82499792-82499811 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 82499039 82499884 82499039 ID=MsG0280011330.01;Name=MsG0280011330.01
Chr2 mRNA 82499039 82499884 82499039 ID=MsG0280011330.01.T01;Parent=MsG0280011330.01;Name=MsG0280011330.01.T01;_AED=0.16;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|281
Chr2 exon 82499039 82499884 82499039 ID=MsG0280011330.01.T01:exon:2931;Parent=MsG0280011330.01.T01
Chr2 CDS 82499039 82499884 82499039 ID=MsG0280011330.01.T01:cds;Parent=MsG0280011330.01.T01
Gene Sequence

>MsG0280011330.01.T01

ATGCTCTCTGGCTGCAAACATCCCAAAACACCATCTTTTTCCTTGGATAACGGCAGAAACATATCATCCTCTAACGCCGTTAACGACAATAACAACAAAATCAATGATGCTGCAACACTAGCTGACGTTGACCGTTTTCTCTTTGAGAATTTCAAGTCTCTATATTTCAAAGATGATGAAGAAACAGAACAAAACAACAACAACAACAAAAACAAAAGAATCTCGGATGAAAAAAACAACGAGGAACCACCAAAACGTATTGGTGGTTCCTGGTTGTTGGAGTCACCGAGATTCATTACAACACCACCTCAAGATCTTTGTGGCTCAGCACGATTCTTCGTGAAACCAGGTAACTCAGGATCATTGATGGAAGATGCACTGTCTCTAACAAACAGTGATGAAGCTGATTCGAGTAATTCGAATAATTCAAATTCATCATCAACAGCATCACCTTCAAAAGAGGTTATTGTAGTAAACCATGATGATCACAATCATCACACTCTTCCTGAAAATTGTGTTGCACTGTTATCATATTCTCCGAGTCCTTACGATGATTTCAGGCGATCAATGCAAGAATTGGTGGAATCAAAGTATGGTAAGATTGAAAACAATCAAAGGAAAATTGATTGGGACTTCATGGAGGAGATTTTGTTTTGTTATCTTAATGTTAATGAGAAAAAATCTCATAAGTTTATATTAAGTGCTTTTGTTGATCTCATCACTGTTATGAGGAAGAATTCAGAGGCTGCTCCGGCGAAGCCGTGTAGCGTGAGAACTGTTAGGATTGGTAGGGAAGTCAGGAAGAAGAAGACCAAGCAGGTTACCATAGAATTTGGTTCCTCTTAG

Protein sequence

>MsG0280011330.01.T01

MLSGCKHPKTPSFSLDNGRNISSSNAVNDNNNKINDAATLADVDRFLFENFKSLYFKDDEETEQNNNNNKNKRISDEKNNEEPPKRIGGSWLLESPRFITTPPQDLCGSARFFVKPGNSGSLMEDALSLTNSDEADSSNSNNSNSSSTASPSKEVIVVNHDDHNHHTLPENCVALLSYSPSPYDDFRRSMQELVESKYGKIENNQRKIDWDFMEEILFCYLNVNEKKSHKFILSAFVDLITVMRKNSEAAPAKPCSVRTVRIGREVRKKKTKQVTIEFGSS*