Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046580.01.T01 | XP_003609161.1 | 91.318 | 311 | 25 | 2 | 7 | 316 | 2 | 311 | 0 | 565 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046580.01.T01 | Q4V3D9 | 48.511 | 235 | 113 | 4 | 42 | 275 | 56 | 283 | 1.19E-67 | 219 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046580.01.T01 | G7JT36 | 91.318 | 311 | 25 | 2 | 7 | 316 | 2 | 311 | 0.0 | 565 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0880046580.01 | 0.819168 | 1.338421e-52 | 1.022205e-49 |
| MsG0880045585.01 | MsG0880046580.01 | 0.814517 | 1.479953e-51 | 9.952007e-49 |
| MsG0880046580.01 | MsG0880046636.01 | 0.987688 | 3.595420e-171 | 1.924618e-164 |
| MsG0880046580.01 | MsG0880046942.01 | 0.815628 | 8.385210e-52 | 5.811857e-49 |
| MsG0180004765.01 | MsG0880046580.01 | 0.807958 | 3.918449e-50 | 2.212363e-47 |
| MsG0780040287.01 | MsG0880046580.01 | 0.803614 | 3.205644e-49 | 1.618209e-46 |
| MsG0780040432.01 | MsG0880046580.01 | 0.809185 | 2.144329e-50 | 1.250556e-47 |
| MsG0880041834.01 | MsG0880046580.01 | 0.800453 | 1.430947e-48 | 6.665366e-46 |
| MsG0880041858.01 | MsG0880046580.01 | 0.808355 | 3.225977e-50 | 1.840646e-47 |
| MsG0880042442.01 | MsG0880046580.01 | 0.822744 | 2.011197e-53 | 1.698179e-50 |
| MsG0880043480.01 | MsG0880046580.01 | 0.812411 | 4.297257e-51 | 2.730453e-48 |
| MsG0380011501.01 | MsG0880046580.01 | 0.800428 | 1.448266e-48 | 6.741948e-46 |
| MsG0680031428.01 | MsG0880046580.01 | 0.806008 | 1.013334e-49 | 5.440334e-47 |
| MsG0680031707.01 | MsG0880046580.01 | 0.805217 | 1.484895e-49 | 7.811397e-47 |
| MsG0480018909.01 | MsG0880046580.01 | 0.823421 | 1.397622e-53 | 1.202748e-50 |
| MsG0380017869.01 | MsG0880046580.01 | 0.822400 | 2.417161e-53 | 2.021146e-50 |
| MsG0580029417.01 | MsG0880046580.01 | 0.803082 | 4.130794e-49 | 2.056824e-46 |
| MsG0280007414.01 | MsG0880046580.01 | 0.831320 | 1.784599e-55 | 1.929807e-52 |
| MsG0280007547.01 | MsG0880046580.01 | 0.829957 | 3.848926e-55 | 3.999238e-52 |
| MsG0280009920.01 | MsG0880046580.01 | 0.806988 | 6.294296e-50 | 3.465036e-47 |
| MsG0680035658.01 | MsG0880046580.01 | 0.849571 | 2.969322e-60 | 5.652771e-57 |
| MsG0680035683.01 | MsG0880046580.01 | 0.808926 | 2.435462e-50 | 1.410446e-47 |
| MsG0780038401.01 | MsG0880046580.01 | 0.810569 | 1.079796e-50 | 6.531530e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880046579.01 | MsG0880046580.01 | PPI |
| MsG0880046635.01 | MsG0880046580.01 | PPI |
| MsG0880046580.01 | MsG0280009252.01 | PPI |
| MsG0880046580.01 | MsG0880046636.01 | PPI |
| MsG0880046634.01 | MsG0880046580.01 | PPI |
| MsG0880046636.01 | MsG0880046580.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046580.01.T01 | MTR_4g112630 | 91.318 | 311 | 25 | 2 | 7 | 316 | 2 | 311 | 0.0 | 565 |
| MsG0880046580.01.T01 | MTR_4g112650 | 79.128 | 321 | 54 | 4 | 7 | 316 | 2 | 320 | 5.65e-180 | 500 |
| MsG0880046580.01.T01 | MTR_4g112620 | 76.875 | 320 | 36 | 3 | 1 | 316 | 1 | 286 | 3.42e-172 | 479 |
| MsG0880046580.01.T01 | MTR_4g112670 | 74.468 | 329 | 63 | 6 | 1 | 316 | 1 | 321 | 4.03e-171 | 478 |
| MsG0880046580.01.T01 | MTR_2g058450 | 54.698 | 298 | 120 | 4 | 30 | 316 | 28 | 321 | 4.05e-104 | 308 |
| MsG0880046580.01.T01 | MTR_0003s0530 | 70.556 | 180 | 37 | 4 | 149 | 316 | 1 | 176 | 3.71e-79 | 239 |
| MsG0880046580.01.T01 | MTR_7g490330 | 44.928 | 276 | 139 | 6 | 42 | 316 | 47 | 310 | 2.23e-71 | 224 |
| MsG0880046580.01.T01 | MTR_7g490350 | 45.957 | 235 | 119 | 4 | 42 | 275 | 53 | 280 | 5.52e-68 | 217 |
| MsG0880046580.01.T01 | MTR_2g058410 | 52.941 | 221 | 91 | 3 | 106 | 316 | 1 | 218 | 7.11e-67 | 209 |
| MsG0880046580.01.T01 | MTR_7g090300 | 41.581 | 291 | 137 | 6 | 6 | 275 | 2 | 280 | 7.28e-66 | 211 |
| MsG0880046580.01.T01 | MTR_5g091980 | 38.462 | 312 | 176 | 6 | 7 | 316 | 11 | 308 | 1.59e-65 | 209 |
| MsG0880046580.01.T01 | MTR_7g090320 | 44.622 | 251 | 129 | 5 | 32 | 275 | 48 | 295 | 2.43e-65 | 211 |
| MsG0880046580.01.T01 | MTR_2g087780 | 43.028 | 251 | 131 | 5 | 29 | 274 | 60 | 303 | 1.15e-62 | 204 |
| MsG0880046580.01.T01 | MTR_7g090310 | 43.750 | 240 | 126 | 4 | 42 | 275 | 58 | 294 | 1.34e-60 | 198 |
| MsG0880046580.01.T01 | MTR_4g050190 | 41.129 | 248 | 133 | 5 | 32 | 274 | 60 | 299 | 2.84e-60 | 197 |
| MsG0880046580.01.T01 | MTR_2g099030 | 38.645 | 251 | 145 | 3 | 30 | 275 | 70 | 316 | 1.67e-54 | 183 |
| MsG0880046580.01.T01 | MTR_4g112840 | 67.327 | 101 | 30 | 1 | 176 | 276 | 56 | 153 | 2.25e-39 | 136 |
| MsG0880046580.01.T01 | MTR_5g077620 | 72.619 | 84 | 21 | 2 | 8 | 91 | 1 | 82 | 2.13e-34 | 123 |
| MsG0880046580.01.T01 | MTR_8g074210 | 39.181 | 171 | 98 | 4 | 103 | 273 | 118 | 282 | 2.94e-27 | 110 |
| MsG0880046580.01.T01 | MTR_8g074170 | 38.596 | 171 | 99 | 4 | 103 | 273 | 118 | 282 | 1.18e-25 | 105 |
| MsG0880046580.01.T01 | MTR_8g074270 | 38.012 | 171 | 100 | 4 | 103 | 273 | 118 | 282 | 2.93e-25 | 104 |
| MsG0880046580.01.T01 | MTR_2g042430 | 31.381 | 239 | 134 | 8 | 40 | 277 | 74 | 283 | 3.45e-24 | 101 |
| MsG0880046580.01.T01 | MTR_2g058430 | 50.485 | 103 | 42 | 1 | 79 | 181 | 43 | 136 | 1.20e-23 | 98.2 |
| MsG0880046580.01.T01 | MTR_2g042370 | 33.133 | 166 | 105 | 4 | 113 | 277 | 112 | 272 | 1.25e-20 | 91.3 |
| MsG0880046580.01.T01 | MTR_2g042420 | 28.033 | 239 | 142 | 8 | 40 | 277 | 55 | 264 | 6.99e-20 | 89.0 |
| MsG0880046580.01.T01 | MTR_8g074320 | 38.462 | 130 | 72 | 4 | 103 | 229 | 95 | 219 | 1.79e-18 | 82.8 |
| MsG0880046580.01.T01 | MTR_1g075865 | 40.541 | 111 | 62 | 2 | 153 | 262 | 4 | 111 | 2.71e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046580.01.T01 | AT1G74020 | 44.218 | 294 | 148 | 8 | 31 | 316 | 27 | 312 | 5.94e-69 | 218 |
| MsG0880046580.01.T01 | AT3G57030 | 48.511 | 235 | 113 | 4 | 42 | 275 | 56 | 283 | 1.21e-68 | 219 |
| MsG0880046580.01.T01 | AT1G74010 | 43.537 | 294 | 149 | 9 | 31 | 316 | 26 | 310 | 6.78e-67 | 213 |
| MsG0880046580.01.T01 | AT1G74000 | 41.438 | 292 | 159 | 7 | 31 | 316 | 29 | 314 | 7.52e-64 | 205 |
| MsG0880046580.01.T01 | AT3G57010 | 38.376 | 271 | 151 | 4 | 11 | 274 | 16 | 277 | 2.02e-63 | 205 |
| MsG0880046580.01.T01 | AT5G22020 | 44.538 | 238 | 122 | 5 | 40 | 274 | 73 | 303 | 4.62e-62 | 202 |
| MsG0880046580.01.T01 | AT5G22020 | 44.538 | 238 | 122 | 5 | 40 | 274 | 73 | 303 | 4.62e-62 | 202 |
| MsG0880046580.01.T01 | AT5G22020 | 44.538 | 238 | 122 | 5 | 40 | 274 | 73 | 303 | 4.62e-62 | 202 |
| MsG0880046580.01.T01 | AT1G08470 | 42.276 | 246 | 132 | 5 | 32 | 274 | 59 | 297 | 4.89e-60 | 197 |
| MsG0880046580.01.T01 | AT3G57020 | 40.000 | 240 | 135 | 2 | 36 | 275 | 47 | 277 | 1.12e-59 | 195 |
| MsG0880046580.01.T01 | AT2G41290 | 43.154 | 241 | 123 | 7 | 42 | 275 | 51 | 284 | 4.34e-58 | 191 |
| MsG0880046580.01.T01 | AT2G41300 | 39.301 | 229 | 130 | 3 | 47 | 275 | 83 | 302 | 4.60e-54 | 181 |
| MsG0880046580.01.T01 | AT3G57020 | 38.075 | 239 | 125 | 2 | 36 | 274 | 47 | 262 | 5.15e-54 | 180 |
| MsG0880046580.01.T01 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 3.88e-53 | 179 |
| MsG0880046580.01.T01 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 3.88e-53 | 179 |
| MsG0880046580.01.T01 | AT3G59530 | 36.090 | 266 | 161 | 3 | 33 | 293 | 75 | 336 | 3.88e-53 | 179 |
| MsG0880046580.01.T01 | AT3G51440 | 27.891 | 294 | 178 | 9 | 20 | 305 | 40 | 307 | 7.83e-23 | 97.8 |
| MsG0880046580.01.T01 | AT3G51430 | 35.028 | 177 | 106 | 5 | 103 | 278 | 113 | 281 | 2.02e-21 | 93.6 |
| MsG0880046580.01.T01 | AT3G51430 | 35.057 | 174 | 104 | 5 | 103 | 275 | 113 | 278 | 9.60e-21 | 92.0 |
| MsG0880046580.01.T01 | AT3G51450 | 30.732 | 205 | 125 | 6 | 103 | 302 | 113 | 305 | 5.24e-20 | 89.7 |
| MsG0880046580.01.T01 | AT3G51420 | 29.353 | 201 | 133 | 5 | 103 | 302 | 113 | 305 | 6.75e-20 | 89.4 |
Find 92 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATTCAAGAGGCCAATTT+TGG | 0.175988 | 8:+75084596 | MsG0880046580.01.T01:CDS |
| AAATAAAATTAACTCATTAT+TGG | 0.210818 | 8:+75083251 | MsG0880046580.01.T01:five_prime_UTR |
| TATGCAGCTGATGCTTATTA+TGG | 0.243078 | 8:+75083769 | MsG0880046580.01.T01:CDS |
| GAATTCCTCTTTATGTTATC+TGG | 0.262678 | 8:-75084580 | None:intergenic |
| CTTGTGCAGGTCCAACTAAT+TGG | 0.268148 | 8:-75083828 | None:intergenic |
| TATTAACTGCAATCCAAAAT+TGG | 0.282710 | 8:-75084609 | None:intergenic |
| AAATCTGAGGAATTTGCTTT+TGG | 0.297207 | 8:-75084535 | None:intergenic |
| GTTGAACATTAATAATTCTA+TGG | 0.304772 | 8:+75083284 | MsG0880046580.01.T01:CDS |
| TTGGGTTGCTTTCCCTCCAT+TGG | 0.315305 | 8:-75083809 | None:intergenic |
| AAATAAACGATTCCAGTGTT+TGG | 0.321439 | 8:-75083889 | None:intergenic |
| TGGGTTGCTTTCCCTCCATT+GGG | 0.331282 | 8:-75083808 | None:intergenic |
| CTGAAGCTTGTTCAGTAATA+TGG | 0.333503 | 8:-75083364 | None:intergenic |
| AGAAATCTTAAATTTGCATC+TGG | 0.338821 | 8:+75084430 | MsG0880046580.01.T01:CDS |
| TAATAGTCTTACTTTGTCTT+CGG | 0.340893 | 8:+75084627 | MsG0880046580.01.T01:CDS |
| GTCTCTCTTGTTGAAGAGTA+TGG | 0.345465 | 8:+75084688 | MsG0880046580.01.T01:CDS |
| TACTTGTTGCCATCTTTGTA+TGG | 0.347946 | 8:+75084810 | MsG0880046580.01.T01:three_prime_UTR |
| CTGTCAAATGCAAGAGAATC+AGG | 0.348024 | 8:-75083408 | None:intergenic |
| AATAGTCTTACTTTGTCTTC+GGG | 0.365443 | 8:+75084628 | MsG0880046580.01.T01:CDS |
| GATTTATTCATGCTACTTGC+TGG | 0.370329 | 8:+75084553 | MsG0880046580.01.T01:CDS |
| CTGTAAAACTATACCATTCT+CGG | 0.373836 | 8:-75084662 | None:intergenic |
| ATGCAGCTGATGCTTATTAT+GGG | 0.375296 | 8:+75083770 | MsG0880046580.01.T01:CDS |
| ATCAGCAAACACGGTAGAAT+TGG | 0.379198 | 8:-75083851 | None:intergenic |
| AATAAACGATTCCAGTGTTT+GGG | 0.380124 | 8:-75083888 | None:intergenic |
| AATTTCTTTATTTAGTCCTT+AGG | 0.387475 | 8:-75084849 | None:intergenic |
| GCAATATGTGGAAGGCCATT+AGG | 0.400762 | 8:+75083715 | MsG0880046580.01.T01:CDS |
| CACTGGTTAGCAGCGCTGTC+TGG | 0.406496 | 8:-75084339 | None:intergenic |
| TGCTTGGATCATAACTCAAT+AGG | 0.410469 | 8:-75084384 | None:intergenic |
| ACTGTTGTCTGGTTGTTGCT+TGG | 0.420207 | 8:-75084400 | None:intergenic |
| GATTTATATATTTACCTCTT+GGG | 0.420261 | 8:-75083525 | None:intergenic |
| TTGTGCAGGTCCAACTAATT+GGG | 0.425364 | 8:-75083827 | None:intergenic |
| TTCTCAGTAACACTGTTGTC+TGG | 0.431970 | 8:-75084411 | None:intergenic |
| TGTGGAAGGCCATTAGGATT+GGG | 0.435505 | 8:+75083721 | MsG0880046580.01.T01:CDS |
| TTGGGTTTCAATCATCAAAC+AGG | 0.444949 | 8:+75083739 | MsG0880046580.01.T01:CDS |
| AGAGTATGGTCTTCTTGAAC+CGG | 0.453051 | 8:+75084702 | MsG0880046580.01.T01:CDS |
| ATGGCCTTCCACATATTGCT+TGG | 0.453479 | 8:-75083711 | None:intergenic |
| CTTGTAAAGGTTGGCCCCAA+TGG | 0.461054 | 8:+75083793 | MsG0880046580.01.T01:CDS |
| ATGTGGAAGGCCATTAGGAT+TGG | 0.461713 | 8:+75083720 | MsG0880046580.01.T01:CDS |
| AAAACTATGCTTACTTGAGC+TGG | 0.464925 | 8:-75083933 | None:intergenic |
| GAAATCTTAAATTTGCATCT+GGG | 0.465666 | 8:+75084431 | MsG0880046580.01.T01:CDS |
| TGGTCCAAGTGAAGGTTTCA+AGG | 0.466762 | 8:+75083485 | MsG0880046580.01.T01:CDS |
| TATTATGGGCTTGTAAAGGT+TGG | 0.470412 | 8:+75083784 | MsG0880046580.01.T01:CDS |
| CAGTAGAAAGATAGCAAGAA+TGG | 0.470940 | 8:-75083322 | None:intergenic |
| ACTAAATAAAGAAATTTGTA+TGG | 0.481210 | 8:+75084856 | MsG0880046580.01.T01:three_prime_UTR |
| AGTGGCATTGGAGATAATTC+AGG | 0.481306 | 8:+75084358 | MsG0880046580.01.T01:CDS |
| TACTCTTGAACCTCACTAGC+CGG | 0.499543 | 8:-75084721 | None:intergenic |
| GGGAAAGCAACCCAATTAGT+TGG | 0.500291 | 8:+75083817 | MsG0880046580.01.T01:CDS |
| GCGCTGCTAACCAGTGGCAT+TGG | 0.502363 | 8:+75084346 | MsG0880046580.01.T01:CDS |
| TGATTGAAACCCAATCCTAA+TGG | 0.505270 | 8:-75083730 | None:intergenic |
| TCTCTTGCATTTGACAGAAA+CGG | 0.511849 | 8:+75083414 | MsG0880046580.01.T01:CDS |
| AATTCTACCGTGTTTGCTGA+TGG | 0.517101 | 8:+75083853 | MsG0880046580.01.T01:CDS |
| TGAATTATCTCCAATGCCAC+TGG | 0.518510 | 8:-75084356 | None:intergenic |
| CTTGTTCAGTAATATGGCTA+TGG | 0.523436 | 8:-75083358 | None:intergenic |
| CCATCAGAAGAAGTAACATA+AGG | 0.525792 | 8:-75083444 | None:intergenic |
| CCTTATGTTACTTCTTCTGA+TGG | 0.527626 | 8:+75083444 | MsG0880046580.01.T01:CDS |
| AAATATGTTGGTCCAAGTGA+AGG | 0.529246 | 8:+75083477 | MsG0880046580.01.T01:CDS |
| GGTGTTAGAGTGACCGAGAA+TGG | 0.530351 | 8:+75084649 | MsG0880046580.01.T01:CDS |
| TCAGAAATCCAAGCAATATG+TGG | 0.532707 | 8:+75083703 | MsG0880046580.01.T01:CDS |
| TGCTTATTATGGGCTTGTAA+AGG | 0.537666 | 8:+75083780 | MsG0880046580.01.T01:CDS |
| GGGGTGGCAGTAAGCGGAGA+TGG | 0.543707 | 8:+75084451 | MsG0880046580.01.T01:CDS |
| ACGGTAGAATTGGCTTGTGC+AGG | 0.544655 | 8:-75083841 | None:intergenic |
| ATTCGGAGAGTGTGGTTGAA+AGG | 0.547509 | 8:+75084511 | MsG0880046580.01.T01:CDS |
| TGATTGCATAAGAAGCAAGC+AGG | 0.548928 | 8:-75084766 | None:intergenic |
| GAAGACCAGATAACATAAAG+AGG | 0.552163 | 8:+75084575 | MsG0880046580.01.T01:CDS |
| AGAGAATCAGGTCCTTTCAC+TGG | 0.553043 | 8:-75083396 | None:intergenic |
| TTAGATGTGGACCCAAACAC+TGG | 0.559091 | 8:+75083877 | MsG0880046580.01.T01:CDS |
| AAATCTTAAATTTGCATCTG+GGG | 0.560132 | 8:+75084432 | MsG0880046580.01.T01:CDS |
| TCAGGTCCTTTCACTGGTGA+TGG | 0.564770 | 8:-75083390 | None:intergenic |
| GTAAAGGTTGGCCCCAATGG+AGG | 0.566207 | 8:+75083796 | MsG0880046580.01.T01:CDS |
| TAAAGGTTGGCCCCAATGGA+GGG | 0.566544 | 8:+75083797 | MsG0880046580.01.T01:CDS |
| TATTCCTTGAAACCTTCACT+TGG | 0.569604 | 8:-75083489 | None:intergenic |
| CATTAATAATTCTATGGTGA+TGG | 0.573040 | 8:+75083290 | MsG0880046580.01.T01:CDS |
| AGAGTACAATGGAACACTCT+AGG | 0.574183 | 8:+75084738 | MsG0880046580.01.T01:CDS |
| TCTTCTTGAACCGGCTAGTG+AGG | 0.578667 | 8:+75084711 | MsG0880046580.01.T01:CDS |
| CTAACACAATTCGGAGAGTG+TGG | 0.584118 | 8:+75084503 | MsG0880046580.01.T01:CDS |
| GTTTGCTGATGGTTTAGATG+TGG | 0.585669 | 8:+75083864 | MsG0880046580.01.T01:CDS |
| GGGTTGCTTTCCCTCCATTG+GGG | 0.591391 | 8:-75083807 | None:intergenic |
| CTGGAATTTAGTGCTAGCTA+TGG | 0.599531 | 8:-75083914 | None:intergenic |
| AGGAACAGGACAATCTGTGA+TGG | 0.601201 | 8:+75083667 | MsG0880046580.01.T01:intron |
| ATCTAAACCATCAGCAAACA+CGG | 0.601997 | 8:-75083860 | None:intergenic |
| GCATCTGGGGTGGCAGTAAG+CGG | 0.604290 | 8:+75084445 | MsG0880046580.01.T01:CDS |
| TCTTAAATTTGCATCTGGGG+TGG | 0.610328 | 8:+75084435 | MsG0880046580.01.T01:CDS |
| AAATCCAAGCAATATGTGGA+AGG | 0.615490 | 8:+75083707 | MsG0880046580.01.T01:CDS |
| GCATTTGACAGAAACGGTGA+AGG | 0.616080 | 8:+75083420 | MsG0880046580.01.T01:CDS |
| CAGCTACCATCACCAGTGAA+AGG | 0.618564 | 8:+75083384 | MsG0880046580.01.T01:CDS |
| AACATAAAGAGGAATTCAAG+AGG | 0.628213 | 8:+75084586 | MsG0880046580.01.T01:CDS |
| GATTGCATAAGAAGCAAGCA+GGG | 0.638280 | 8:-75084765 | None:intergenic |
| AAGTAGCATGAATAAATCTG+AGG | 0.664600 | 8:-75084548 | None:intergenic |
| CAGACAGCGCTGCTAACCAG+TGG | 0.665038 | 8:+75084340 | MsG0880046580.01.T01:CDS |
| ATGCTTACACTTCTCCCAAG+AGG | 0.667804 | 8:+75083511 | MsG0880046580.01.T01:CDS |
| AGTGAGGTTCAAGAGTACAA+TGG | 0.673039 | 8:+75084727 | MsG0880046580.01.T01:CDS |
| ATTATATTATACACTCACAG+TGG | 0.734184 | 8:+75084923 | MsG0880046580.01.T01:three_prime_UTR |
| GTACAATGGAACACTCTAGG+TGG | 0.756114 | 8:+75084741 | MsG0880046580.01.T01:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAATTACTATGTAAA+TGG | - | Chr8:75083586-75083605 | None:intergenic | 10.0% |
| !! | AAATAAAATTAACTCATTAT+TGG | + | Chr8:75083251-75083270 | MsG0880046580.01.T01:five_prime_UTR | 10.0% |
| !!! | AATGATTGTTTTTTATTTTT+TGG | + | Chr8:75083641-75083660 | MsG0880046580.01.T01:intron | 10.0% |
| !! | ACTAAATAAAGAAATTTGTA+TGG | + | Chr8:75084856-75084875 | MsG0880046580.01.T01:three_prime_UTR | 15.0% |
| !! | TGATAATTTATGAGAAAAAT+TGG | - | Chr8:75083992-75084011 | None:intergenic | 15.0% |
| !! | TTCAATTATGTTATTGTTTA+TGG | + | Chr8:75084209-75084228 | MsG0880046580.01.T01:intron | 15.0% |
| !!! | ATGAGTTAATTTTATTTTTC+AGG | - | Chr8:75083248-75083267 | None:intergenic | 15.0% |
| !!! | TTAATTTTATTTTTCAGGTA+AGG | - | Chr8:75083243-75083262 | None:intergenic | 15.0% |
| !! | CTATGAAAAAAATTTACCTA+AGG | + | Chr8:75084879-75084898 | MsG0880046580.01.T01:three_prime_UTR | 20.0% |
| !! | GATTTATATATTTACCTCTT+GGG | - | Chr8:75083528-75083547 | None:intergenic | 20.0% |
| !! | GTTGAACATTAATAATTCTA+TGG | + | Chr8:75083284-75083303 | MsG0880046580.01.T01:CDS | 20.0% |
| !! | TGATTTATATATTTACCTCT+TGG | - | Chr8:75083529-75083548 | None:intergenic | 20.0% |
| !!! | AATATTTTGCTAACACAATT+CGG | + | Chr8:75084494-75084513 | MsG0880046580.01.T01:CDS | 20.0% |
| !!! | AATTTCTTTATTTAGTCCTT+AGG | - | Chr8:75084852-75084871 | None:intergenic | 20.0% |
| !!! | ATTTTCTTTATTTAGTCCTT+AGG | - | Chr8:75084898-75084917 | None:intergenic | 20.0% |
| !!! | TGTTTTTTATTTTTTGGTTC+AGG | + | Chr8:75083647-75083666 | MsG0880046580.01.T01:intron | 20.0% |
| ! | AAATCTTAAATTTGCATCTG+GGG | + | Chr8:75084432-75084451 | MsG0880046580.01.T01:CDS | 25.0% |
| ! | AGAAATCTTAAATTTGCATC+TGG | + | Chr8:75084430-75084449 | MsG0880046580.01.T01:CDS | 25.0% |
| ! | ATTATATTATACACTCACAG+TGG | + | Chr8:75084923-75084942 | MsG0880046580.01.T01:three_prime_UTR | 25.0% |
| ! | CATTAATAATTCTATGGTGA+TGG | + | Chr8:75083290-75083309 | MsG0880046580.01.T01:CDS | 25.0% |
| ! | CTATGAAAAAAAATCACCTA+AGG | + | Chr8:75084833-75084852 | MsG0880046580.01.T01:three_prime_UTR | 25.0% |
| ! | CTTTCTTTCCTCAAATAATT+TGG | + | Chr8:75084182-75084201 | MsG0880046580.01.T01:intron | 25.0% |
| ! | GAAATCTTAAATTTGCATCT+GGG | + | Chr8:75084431-75084450 | MsG0880046580.01.T01:CDS | 25.0% |
| ! | TATTAACTGCAATCCAAAAT+TGG | - | Chr8:75084612-75084631 | None:intergenic | 25.0% |
| !! | GGAAGAATTTTCAAATATGT+TGG | + | Chr8:75083465-75083484 | MsG0880046580.01.T01:CDS | 25.0% |
| !! | GTTTAGTTTCAAAATCATAG+AGG | + | Chr8:75084246-75084265 | MsG0880046580.01.T01:intron | 25.0% |
| !! | TAATAGTCTTACTTTGTCTT+CGG | + | Chr8:75084627-75084646 | MsG0880046580.01.T01:CDS | 25.0% |
| AAATAAACGATTCCAGTGTT+TGG | - | Chr8:75083892-75083911 | None:intergenic | 30.0% | |
| AACATAAAGAGGAATTCAAG+AGG | + | Chr8:75084586-75084605 | MsG0880046580.01.T01:CDS | 30.0% | |
| AAGTAGCATGAATAAATCTG+AGG | - | Chr8:75084551-75084570 | None:intergenic | 30.0% | |
| AATAAACGATTCCAGTGTTT+GGG | - | Chr8:75083891-75083910 | None:intergenic | 30.0% | |
| CTGTAAAACTATACCATTCT+CGG | - | Chr8:75084665-75084684 | None:intergenic | 30.0% | |
| CTTTCCTCAAATAATTTGGA+GGG | + | Chr8:75084186-75084205 | MsG0880046580.01.T01:intron | 30.0% | |
| GAATTCCTCTTTATGTTATC+TGG | - | Chr8:75084583-75084602 | None:intergenic | 30.0% | |
| GCAACAAGTAAAAAACTCTA+GGG | - | Chr8:75084800-75084819 | None:intergenic | 30.0% | |
| TCTTTCCTCAAATAATTTGG+AGG | + | Chr8:75084185-75084204 | MsG0880046580.01.T01:intron | 30.0% | |
| ! | AATAGTCTTACTTTGTCTTC+GGG | + | Chr8:75084628-75084647 | MsG0880046580.01.T01:CDS | 30.0% |
| ! | TTTTCATAGCCATACAAAGA+TGG | - | Chr8:75084822-75084841 | None:intergenic | 30.0% |
| !! | AAATCTGAGGAATTTGCTTT+TGG | - | Chr8:75084538-75084557 | None:intergenic | 30.0% |
| !!! | TTATTTTTTGGTTCAGGAAC+AGG | + | Chr8:75083653-75083672 | MsG0880046580.01.T01:intron | 30.0% |
| AAAACTATGCTTACTTGAGC+TGG | - | Chr8:75083936-75083955 | None:intergenic | 35.0% | |
| AAATATGTTGGTCCAAGTGA+AGG | + | Chr8:75083477-75083496 | MsG0880046580.01.T01:CDS | 35.0% | |
| AAATCCAAGCAATATGTGGA+AGG | + | Chr8:75083707-75083726 | MsG0880046580.01.T01:CDS | 35.0% | |
| ATCTAAACCATCAGCAAACA+CGG | - | Chr8:75083863-75083882 | None:intergenic | 35.0% | |
| ATGCAGCTGATGCTTATTAT+GGG | + | Chr8:75083770-75083789 | MsG0880046580.01.T01:CDS | 35.0% | |
| ATGGTCACTTGATTCATGTT+AGG | - | Chr8:75083567-75083586 | None:intergenic | 35.0% | |
| CAGTAGAAAGATAGCAAGAA+TGG | - | Chr8:75083325-75083344 | None:intergenic | 35.0% | |
| CCATCAGAAGAAGTAACATA+AGG | - | Chr8:75083447-75083466 | None:intergenic | 35.0% | |
| CTTGTTCAGTAATATGGCTA+TGG | - | Chr8:75083361-75083380 | None:intergenic | 35.0% | |
| GAAGACCAGATAACATAAAG+AGG | + | Chr8:75084575-75084594 | MsG0880046580.01.T01:CDS | 35.0% | |
| GGCAACAAGTAAAAAACTCT+AGG | - | Chr8:75084801-75084820 | None:intergenic | 35.0% | |
| TATGCAGCTGATGCTTATTA+TGG | + | Chr8:75083769-75083788 | MsG0880046580.01.T01:CDS | 35.0% | |
| TATTATGGGCTTGTAAAGGT+TGG | + | Chr8:75083784-75083803 | MsG0880046580.01.T01:CDS | 35.0% | |
| TATTCCTTGAAACCTTCACT+TGG | - | Chr8:75083492-75083511 | None:intergenic | 35.0% | |
| TCAGAAATCCAAGCAATATG+TGG | + | Chr8:75083703-75083722 | MsG0880046580.01.T01:CDS | 35.0% | |
| TCTCTTGCATTTGACAGAAA+CGG | + | Chr8:75083414-75083433 | MsG0880046580.01.T01:CDS | 35.0% | |
| TGAACCCTCCAAATTATTTG+AGG | - | Chr8:75084193-75084212 | None:intergenic | 35.0% | |
| TGATTGAAACCCAATCCTAA+TGG | - | Chr8:75083733-75083752 | None:intergenic | 35.0% | |
| TGCTTATTATGGGCTTGTAA+AGG | + | Chr8:75083780-75083799 | MsG0880046580.01.T01:CDS | 35.0% | |
| TGCTTGGATCATAACTCAAT+AGG | - | Chr8:75084387-75084406 | None:intergenic | 35.0% | |
| TGGTCACTTGATTCATGTTA+GGG | - | Chr8:75083566-75083585 | None:intergenic | 35.0% | |
| TTGGGTTTCAATCATCAAAC+AGG | + | Chr8:75083739-75083758 | MsG0880046580.01.T01:CDS | 35.0% | |
| ! | CCTTATGTTACTTCTTCTGA+TGG | + | Chr8:75083444-75083463 | MsG0880046580.01.T01:CDS | 35.0% |
| ! | CTGAAGCTTGTTCAGTAATA+TGG | - | Chr8:75083367-75083386 | None:intergenic | 35.0% |
| ! | GATTTATTCATGCTACTTGC+TGG | + | Chr8:75084553-75084572 | MsG0880046580.01.T01:CDS | 35.0% |
| ! | TACTTGTTGCCATCTTTGTA+TGG | + | Chr8:75084810-75084829 | MsG0880046580.01.T01:three_prime_UTR | 35.0% |
| AGAGTACAATGGAACACTCT+AGG | + | Chr8:75084738-75084757 | MsG0880046580.01.T01:CDS | 40.0% | |
| AGAGTATGGTCTTCTTGAAC+CGG | + | Chr8:75084702-75084721 | MsG0880046580.01.T01:CDS | 40.0% | |
| ATCAGCAAACACGGTAGAAT+TGG | - | Chr8:75083854-75083873 | None:intergenic | 40.0% | |
| CTGGAATTTAGTGCTAGCTA+TGG | - | Chr8:75083917-75083936 | None:intergenic | 40.0% | |
| CTGTCAAATGCAAGAGAATC+AGG | - | Chr8:75083411-75083430 | None:intergenic | 40.0% | |
| GATTGCATAAGAAGCAAGCA+GGG | - | Chr8:75084768-75084787 | None:intergenic | 40.0% | |
| GTCTCTCTTGTTGAAGAGTA+TGG | + | Chr8:75084688-75084707 | MsG0880046580.01.T01:CDS | 40.0% | |
| TCTTAAATTTGCATCTGGGG+TGG | + | Chr8:75084435-75084454 | MsG0880046580.01.T01:CDS | 40.0% | |
| TGAATTATCTCCAATGCCAC+TGG | - | Chr8:75084359-75084378 | None:intergenic | 40.0% | |
| TGATTGCATAAGAAGCAAGC+AGG | - | Chr8:75084769-75084788 | None:intergenic | 40.0% | |
| TTCTCAGTAACACTGTTGTC+TGG | - | Chr8:75084414-75084433 | None:intergenic | 40.0% | |
| TTGTGCAGGTCCAACTAATT+GGG | - | Chr8:75083830-75083849 | None:intergenic | 40.0% | |
| ! | AATTCTACCGTGTTTGCTGA+TGG | + | Chr8:75083853-75083872 | MsG0880046580.01.T01:CDS | 40.0% |
| ! | AGTGAGGTTCAAGAGTACAA+TGG | + | Chr8:75084727-75084746 | MsG0880046580.01.T01:CDS | 40.0% |
| ! | AGTGGCATTGGAGATAATTC+AGG | + | Chr8:75084358-75084377 | MsG0880046580.01.T01:CDS | 40.0% |
| ! | GGAATTCAAGAGGCCAATTT+TGG | + | Chr8:75084596-75084615 | MsG0880046580.01.T01:CDS | 40.0% |
| ! | GTTTGCTGATGGTTTAGATG+TGG | + | Chr8:75083864-75083883 | MsG0880046580.01.T01:CDS | 40.0% |
| ACTGTTGTCTGGTTGTTGCT+TGG | - | Chr8:75084403-75084422 | None:intergenic | 45.0% | |
| AGAGAATCAGGTCCTTTCAC+TGG | - | Chr8:75083399-75083418 | None:intergenic | 45.0% | |
| AGCATGCGTCACAGATGTTA+GGG | - | Chr8:75084148-75084167 | None:intergenic | 45.0% | |
| AGGAACAGGACAATCTGTGA+TGG | + | Chr8:75083667-75083686 | MsG0880046580.01.T01:intron | 45.0% | |
| ATGCTTACACTTCTCCCAAG+AGG | + | Chr8:75083511-75083530 | MsG0880046580.01.T01:CDS | 45.0% | |
| ATGGCCTTCCACATATTGCT+TGG | - | Chr8:75083714-75083733 | None:intergenic | 45.0% | |
| ATGTGGAAGGCCATTAGGAT+TGG | + | Chr8:75083720-75083739 | MsG0880046580.01.T01:CDS | 45.0% | |
| CTAACACAATTCGGAGAGTG+TGG | + | Chr8:75084503-75084522 | MsG0880046580.01.T01:CDS | 45.0% | |
| CTTGTGCAGGTCCAACTAAT+TGG | - | Chr8:75083831-75083850 | None:intergenic | 45.0% | |
| GCAATATGTGGAAGGCCATT+AGG | + | Chr8:75083715-75083734 | MsG0880046580.01.T01:CDS | 45.0% | |
| GCATTTGACAGAAACGGTGA+AGG | + | Chr8:75083420-75083439 | MsG0880046580.01.T01:CDS | 45.0% | |
| GGGAAAGCAACCCAATTAGT+TGG | + | Chr8:75083817-75083836 | MsG0880046580.01.T01:CDS | 45.0% | |
| GTACAATGGAACACTCTAGG+TGG | + | Chr8:75084741-75084760 | MsG0880046580.01.T01:exon | 45.0% | |
| TACTCTTGAACCTCACTAGC+CGG | - | Chr8:75084724-75084743 | None:intergenic | 45.0% | |
| TGGTCCAAGTGAAGGTTTCA+AGG | + | Chr8:75083485-75083504 | MsG0880046580.01.T01:CDS | 45.0% | |
| TGTGGAAGGCCATTAGGATT+GGG | + | Chr8:75083721-75083740 | MsG0880046580.01.T01:CDS | 45.0% | |
| TTAGATGTGGACCCAAACAC+TGG | + | Chr8:75083877-75083896 | MsG0880046580.01.T01:CDS | 45.0% | |
| ! | ATTCGGAGAGTGTGGTTGAA+AGG | + | Chr8:75084511-75084530 | MsG0880046580.01.T01:CDS | 45.0% |
| CAGCTACCATCACCAGTGAA+AGG | + | Chr8:75083384-75083403 | MsG0880046580.01.T01:CDS | 50.0% | |
| GAGCATGCGTCACAGATGTT+AGG | - | Chr8:75084149-75084168 | None:intergenic | 50.0% | |
| GCATGCGTCACAGATGTTAG+GGG | - | Chr8:75084147-75084166 | None:intergenic | 50.0% | |
| GGTGTTAGAGTGACCGAGAA+TGG | + | Chr8:75084649-75084668 | MsG0880046580.01.T01:CDS | 50.0% | |
| TCAGGTCCTTTCACTGGTGA+TGG | - | Chr8:75083393-75083412 | None:intergenic | 50.0% | |
| TCTTCTTGAACCGGCTAGTG+AGG | + | Chr8:75084711-75084730 | MsG0880046580.01.T01:CDS | 50.0% | |
| ! | CTTGTAAAGGTTGGCCCCAA+TGG | + | Chr8:75083793-75083812 | MsG0880046580.01.T01:CDS | 50.0% |
| ! | TAAAGGTTGGCCCCAATGGA+GGG | + | Chr8:75083797-75083816 | MsG0880046580.01.T01:CDS | 50.0% |
| ! | TGGGTTGCTTTCCCTCCATT+GGG | - | Chr8:75083811-75083830 | None:intergenic | 50.0% |
| ! | TTGGGTTGCTTTCCCTCCAT+TGG | - | Chr8:75083812-75083831 | None:intergenic | 50.0% |
| !! | ACGGTAGAATTGGCTTGTGC+AGG | - | Chr8:75083844-75083863 | None:intergenic | 50.0% |
| ! | GGGTTGCTTTCCCTCCATTG+GGG | - | Chr8:75083810-75083829 | None:intergenic | 55.0% |
| ! | GTAAAGGTTGGCCCCAATGG+AGG | + | Chr8:75083796-75083815 | MsG0880046580.01.T01:CDS | 55.0% |
| CAGACAGCGCTGCTAACCAG+TGG | + | Chr8:75084340-75084359 | MsG0880046580.01.T01:CDS | 60.0% | |
| GCGCTGCTAACCAGTGGCAT+TGG | + | Chr8:75084346-75084365 | MsG0880046580.01.T01:CDS | 60.0% | |
| ! | CACTGGTTAGCAGCGCTGTC+TGG | - | Chr8:75084342-75084361 | None:intergenic | 60.0% |
| ! | GCATCTGGGGTGGCAGTAAG+CGG | + | Chr8:75084445-75084464 | MsG0880046580.01.T01:CDS | 60.0% |
| GGGGTGGCAGTAAGCGGAGA+TGG | + | Chr8:75084451-75084470 | MsG0880046580.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 75083237 | 75084950 | 75083237 | ID=MsG0880046580.01;Name=MsG0880046580.01 |
| Chr8 | mRNA | 75083237 | 75084950 | 75083237 | ID=MsG0880046580.01.T01;Parent=MsG0880046580.01;Name=MsG0880046580.01.T01;_AED=0.36;_eAED=0.36;_QI=45|1|1|1|1|1|3|191|316 |
| Chr8 | exon | 75083237 | 75083532 | 75083237 | ID=MsG0880046580.01.T01:exon:31467;Parent=MsG0880046580.01.T01 |
| Chr8 | exon | 75083669 | 75083941 | 75083669 | ID=MsG0880046580.01.T01:exon:31468;Parent=MsG0880046580.01.T01 |
| Chr8 | exon | 75084333 | 75084950 | 75084333 | ID=MsG0880046580.01.T01:exon:31469;Parent=MsG0880046580.01.T01 |
| Chr8 | five_prime_UTR | 75083237 | 75083281 | 75083237 | ID=MsG0880046580.01.T01:five_prime_utr;Parent=MsG0880046580.01.T01 |
| Chr8 | CDS | 75083282 | 75083532 | 75083282 | ID=MsG0880046580.01.T01:cds;Parent=MsG0880046580.01.T01 |
| Chr8 | CDS | 75083669 | 75083941 | 75083669 | ID=MsG0880046580.01.T01:cds;Parent=MsG0880046580.01.T01 |
| Chr8 | CDS | 75084333 | 75084759 | 75084333 | ID=MsG0880046580.01.T01:cds;Parent=MsG0880046580.01.T01 |
| Chr8 | three_prime_UTR | 75084760 | 75084950 | 75084760 | ID=MsG0880046580.01.T01:three_prime_utr;Parent=MsG0880046580.01.T01 |
| Gene Sequence |
| Protein sequence |
