Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041834.01.T01 | XP_003604258.1 | 67.206 | 247 | 41 | 4 | 213 | 422 | 132 | 375 | 2.78E-99 | 311 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0880041834.01 | 0.808499 | 3.005252e-50 | 1.721118e-47 |
| MsG0080048787.01 | MsG0880041834.01 | 0.804273 | 2.338452e-49 | 1.200566e-46 |
| MsG0180000210.01 | MsG0880041834.01 | 0.800363 | 1.492897e-48 | 6.938291e-46 |
| MsG0180000404.01 | MsG0880041834.01 | 0.837394 | 5.333539e-57 | 6.918324e-54 |
| MsG0180000777.01 | MsG0880041834.01 | 0.812052 | 5.146111e-51 | 3.237934e-48 |
| MsG0180000782.01 | MsG0880041834.01 | 0.838703 | 2.456943e-57 | 3.317722e-54 |
| MsG0180000784.01 | MsG0880041834.01 | 0.801105 | 1.053168e-48 | 4.987849e-46 |
| MsG0180000785.01 | MsG0880041834.01 | 0.809126 | 2.207293e-50 | 1.285259e-47 |
| MsG0180000787.01 | MsG0880041834.01 | 0.816160 | 6.382492e-52 | 4.488222e-49 |
| MsG0180000814.01 | MsG0880041834.01 | 0.807853 | 4.126179e-50 | 2.323079e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880041834.01.T01 | MTR_4g007180 | 67.206 | 247 | 41 | 4 | 213 | 422 | 132 | 375 | 3.36e-103 | 311 |
| MsG0880041834.01.T01 | MTR_2g105100 | 53.846 | 247 | 74 | 3 | 213 | 422 | 152 | 395 | 3.06e-78 | 248 |
| MsG0880041834.01.T01 | MTR_5g009640 | 52.551 | 196 | 55 | 3 | 255 | 422 | 169 | 354 | 1.81e-55 | 187 |
| MsG0880041834.01.T01 | MTR_5g072370 | 68.504 | 127 | 36 | 1 | 80 | 202 | 1 | 127 | 7.66e-51 | 170 |
| MsG0880041834.01.T01 | MTR_5g067630 | 52.000 | 175 | 67 | 2 | 80 | 237 | 1 | 175 | 3.40e-49 | 166 |
| MsG0880041834.01.T01 | MTR_5g044895 | 66.929 | 127 | 38 | 1 | 80 | 202 | 1 | 127 | 1.69e-48 | 164 |
| MsG0880041834.01.T01 | MTR_5g063995 | 51.429 | 175 | 68 | 2 | 80 | 237 | 1 | 175 | 2.16e-48 | 164 |
| MsG0880041834.01.T01 | MTR_8g470030 | 66.406 | 128 | 39 | 1 | 80 | 203 | 1 | 128 | 1.45e-47 | 162 |
| MsG0880041834.01.T01 | MTR_3g060830 | 65.354 | 127 | 40 | 1 | 80 | 202 | 1 | 127 | 4.86e-47 | 160 |
| MsG0880041834.01.T01 | MTR_6g043270 | 65.354 | 127 | 40 | 1 | 80 | 202 | 1 | 127 | 7.51e-47 | 160 |
Find 74 sgRNAs with CRISPR-Local
Find 593 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATTACAGCTGCATGTAG+TGG | 0.532984 | 8:-22865 | MsG0880041834.01.T01:CDS |
| AACCAGGTAGTGATGGTGAA+GGG | 0.627173 | 8:-27200 | MsG0880041834.01.T01:CDS |
| AACCAGTCAATGCTGGATGC+AGG | 0.515256 | 8:+21953 | None:intergenic |
| AAGACATACCTTGGCAAAGG+AGG | 0.582045 | 8:+30506 | None:intergenic |
| AAGTGCAGCTTCCATTCCAT+TGG | 0.525636 | 8:-21674 | MsG0880041834.01.T01:CDS |
| AATGCAGCATATAAAGAAGA+AGG | 0.507598 | 8:-22130 | MsG0880041834.01.T01:CDS |
| AATGCATCAGACTCAATTAA+GGG | 0.492850 | 8:-22100 | MsG0880041834.01.T01:intron |
| ACATGAGTCTCAGAGGAAGC+TGG | 0.558884 | 8:-27051 | MsG0880041834.01.T01:CDS |
| ACTGATTCATCTAAAGCCAA+TGG | 0.554412 | 8:+21658 | None:intergenic |
| AGAAAGCACATGAGTCTCAG+AGG | 0.627822 | 8:-27058 | MsG0880041834.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCAAAGAATCATAACTC+TGG | + | Chr8:23403-23422 | None:intergenic | 30.0% | |
| AAATCAGTCAAACCTTGTTA+GGG | + | Chr8:23439-23458 | None:intergenic | 30.0% | |
| AAATCTCTTAGAGATTAGGT+CGG | - | Chr8:23996-24015 | MsG0880041834.01.T01:intron | 30.0% | |
| AACATTCAGCAATGCATTAA+GGG | + | Chr8:26438-26457 | None:intergenic | 30.0% | |
| AACCAAAGAATCATAACTCT+GGG | + | Chr8:23402-23421 | None:intergenic | 30.0% | |
| AACTGTTACTAAACTGGAAT+AGG | + | Chr8:26772-26791 | None:intergenic | 30.0% | |
| AAGATTTGCAATGAACCTTA+TGG | + | Chr8:26898-26917 | None:intergenic | 30.0% | |
| AATGCAGCATATAAAGAAGA+AGG | - | Chr8:30023-30042 | MsG0880041834.01.T01:intron | 30.0% | |
| AATGCATCAGACTCAATTAA+GGG | - | Chr8:30053-30072 | MsG0880041834.01.T01:intron | 30.0% | |
| AATTTGCAACCAACTAAGTT+TGG | - | Chr8:27240-27259 | MsG0880041834.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 21552 | 30623 | 21552 | ID=MsG0880041834.01;Name=MsG0880041834.01 |
| Chr8 | mRNA | 21552 | 30623 | 21552 | ID=MsG0880041834.01.T01;Parent=MsG0880041834.01;Name=MsG0880041834.01.T01;_AED=0.64;_eAED=0.67;_QI=0|0|0|0.9|0.66|0.6|10|0|427 |
| Chr8 | exon | 30515 | 30623 | 30515 | ID=MsG0880041834.01.T01:exon:322;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 28604 | 28729 | 28604 | ID=MsG0880041834.01.T01:exon:323;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 27003 | 27353 | 27003 | ID=MsG0880041834.01.T01:exon:324;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 23169 | 23220 | 23169 | ID=MsG0880041834.01.T01:exon:325;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 22814 | 22930 | 22814 | ID=MsG0880041834.01.T01:exon:326;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 22624 | 22741 | 22624 | ID=MsG0880041834.01.T01:exon:327;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 22377 | 22441 | 22377 | ID=MsG0880041834.01.T01:exon:328;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 22101 | 22159 | 22101 | ID=MsG0880041834.01.T01:exon:329;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 21940 | 22018 | 21940 | ID=MsG0880041834.01.T01:exon:330;Parent=MsG0880041834.01.T01 |
| Chr8 | exon | 21552 | 21759 | 21552 | ID=MsG0880041834.01.T01:exon:331;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 30515 | 30623 | 30515 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 28604 | 28729 | 28604 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 27003 | 27353 | 27003 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 23169 | 23220 | 23169 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 22814 | 22930 | 22814 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 22624 | 22741 | 22624 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 22377 | 22441 | 22377 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 22101 | 22159 | 22101 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 21940 | 22018 | 21940 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Chr8 | CDS | 21552 | 21759 | 21552 | ID=MsG0880041834.01.T01:cds;Parent=MsG0880041834.01.T01 |
| Gene Sequence |
>MsG0880041834.01.T01 ATGTATCTACCAACTTCACTCCTTCATCTTCCCTCTTATCACCTTCCATTTCCTCAAAACTTGTTTGCTTTAGTGCTCACAACCATGATTCCCAACCTCCTTTGCCAAGGTTTGACTGATTTGCTTGATGTTATGGTTGCAGGTGGAGAAAACTTGACCACTTTATCAGTAGAGGCAGCAAGGAAAGCTCTTGAGATGGCAAAGGTTGATCCAGCTGATGTTGACCTAATTGGTGCTATGATAGACAGGGTACTCCTCTATGACACAGAAGAGATTGCTAAGTTGCAGTATGAAAAATATATGCTATCTTTTAAGATCAAAATTTTGGACAACAAAATCTCGAGTTGTGATCATCATGAGCAAATCAACCAGGTAGTGATGGTGAAGGGAAGAAAGAGGATGATCAACATCGATTACAATACTAAACTTCAGAATATTTTAGATGATTTCTTGGTGTCAAATCAAGTTTCATTTGAGAAGTTTGATGTTCAGTGTGGTGATCTTGTTGAGAAAGCACATGAGTCTCAGAGGAAGCTGGTTCAGATGAAGAGTGAGTGTCACAAGGTGAGGTGGAAGAAAAACCCAAAGATCTATCATGTTCATCTACTTCAGATGATCTTTTTGGCACTGGTCCTCAGATTTCAAAACAGCTTGGCTGCCAAAAAAAATCCAGCAGCTTATAACATTACAGCTGCATGTAGTGGATTTATCTTTGGTTTAATATCAGCTGCTAGTCACATTAGGGGTATGAATCAGAGCAGCATTTCAAATGTTCTTGTAATCGGAACTGATGCTCTTTCGAGATATATCGATTGGACCGACAGAAGTTCATGTATTTTGTTTGGAGATGCTGCTGGTGCTGTTCTAGTACAGGCGTGCAACTACGAGGAAGATTGTTTATTTAGTTTTGATTTGAATAGTGATGGTGATGGATATATGCATCTGAATGCAGCATATAAAGAAGAAGGGGCTAATAATGCATCAGACTCAATTAAGGAGTGTGCAAAGTCAATTGAGTCAGCACTTCAAAGGCTGGTCTACCTGCATCCAGCATTGACTGGTTACTTCTCCATCAGTGGCAAACAAATTAGAATTCCTCCAGAAAAAGTGATATCAAATGTGGCTGATTATGGTAATACAAGTGCAGCTTCCATTCCATTGGCTTTAGATGAATCAGTTAGAAGTGGAAAGATTAAGGAAGGACAAATTATTGCTGCTTCTGGCTTTGGTGCTGGTCTTACTTGGGGATCAGCTATTTTTCAATGGGATAAGGTCTTACTTTGA |
| Protein sequence |
>MsG0880041834.01.T01 MYLPTSLLHLPSYHLPFPQNLFALVLTTMIPNLLCQGLTDLLDVMVAGGENLTTLSVEAARKALEMAKVDPADVDLIGAMIDRVLLYDTEEIAKLQYEKYMLSFKIKILDNKISSCDHHEQINQVVMVKGRKRMINIDYNTKLQNILDDFLVSNQVSFEKFDVQCGDLVEKAHESQRKLVQMKSECHKVRWKKNPKIYHVHLLQMIFLALVLRFQNSLAAKKNPAAYNITAACSGFIFGLISAASHIRGMNQSSISNVLVIGTDALSRYIDWTDRSSCILFGDAAGAVLVQACNYEEDCLFSFDLNSDGDGYMHLNAAYKEEGANNASDSIKECAKSIESALQRLVYLHPALTGYFSISGKQIRIPPEKVISNVADYGNTSAASIPLALDESVRSGKIKEGQIIAASGFGAGLTWGSAIFQWDKVLL* |
