Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047360.01.T01 | XP_013458424.1 | 96.319 | 163 | 5 | 1 | 1 | 163 | 106 | 267 | 2.75E-110 | 325 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047360.01.T01 | Q9SIP1 | 54.194 | 155 | 68 | 2 | 1 | 153 | 102 | 255 | 1.01E-50 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047360.01.T01 | A0A072V2W0 | 96.319 | 163 | 5 | 1 | 1 | 163 | 106 | 267 | 1.32e-110 | 325 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049013.01 | MsG0880047360.01 | 0.841350 | 5.012140e-58 | 7.344021e-55 |
| MsG0080049059.01 | MsG0880047360.01 | -0.809057 | 2.284256e-50 | 1.327558e-47 |
| MsG0180001551.01 | MsG0880047360.01 | -0.810534 | 1.099141e-50 | 6.642097e-48 |
| MsG0180001795.01 | MsG0880047360.01 | -0.805051 | 1.608879e-49 | 8.427626e-47 |
| MsG0180002489.01 | MsG0880047360.01 | -0.809653 | 1.701122e-50 | 1.004548e-47 |
| MsG0180002491.01 | MsG0880047360.01 | -0.802767 | 4.799690e-49 | 2.370973e-46 |
| MsG0180003088.01 | MsG0880047360.01 | -0.810875 | 9.273581e-51 | 5.655831e-48 |
| MsG0180003409.01 | MsG0880047360.01 | -0.841109 | 5.798010e-58 | 8.431888e-55 |
| MsG0180003864.01 | MsG0880047360.01 | -0.804518 | 2.078347e-49 | 1.073762e-46 |
| MsG0180004079.01 | MsG0880047360.01 | -0.804089 | 2.553426e-49 | 1.304830e-46 |
| MsG0180004566.01 | MsG0880047360.01 | -0.833579 | 4.917580e-56 | 5.687015e-53 |
| MsG0180006069.01 | MsG0880047360.01 | -0.845588 | 3.698157e-59 | 6.192189e-56 |
| MsG0280006411.01 | MsG0880047360.01 | -0.800739 | 1.251405e-48 | 5.871500e-46 |
| MsG0780040442.01 | MsG0880047360.01 | -0.805517 | 1.284809e-49 | 6.810883e-47 |
| MsG0780040699.01 | MsG0880047360.01 | 0.824354 | 8.448975e-54 | 7.465124e-51 |
| MsG0780041357.01 | MsG0880047360.01 | -0.817985 | 2.481455e-52 | 1.834391e-49 |
| MsG0880041883.01 | MsG0880047360.01 | -0.806612 | 7.560381e-50 | 4.121680e-47 |
| MsG0880042294.01 | MsG0880047360.01 | -0.803990 | 2.677384e-49 | 1.364745e-46 |
| MsG0880043465.01 | MsG0880047360.01 | -0.819647 | 1.040547e-52 | 8.054140e-50 |
| MsG0280011120.01 | MsG0880047360.01 | 0.816073 | 6.674302e-52 | 4.682407e-49 |
| MsG0480020065.01 | MsG0880047360.01 | -0.807835 | 4.163610e-50 | 2.343086e-47 |
| MsG0480020090.01 | MsG0880047360.01 | -0.813197 | 2.890858e-51 | 1.875409e-48 |
| MsG0480021448.01 | MsG0880047360.01 | -0.828647 | 8.003055e-55 | 8.003225e-52 |
| MsG0480021453.01 | MsG0880047360.01 | -0.812963 | 3.253929e-51 | 2.097654e-48 |
| MsG0480021464.01 | MsG0880047360.01 | -0.801785 | 7.641649e-49 | 3.681538e-46 |
| MsG0480022447.01 | MsG0880047360.01 | 0.801632 | 8.217048e-49 | 3.943296e-46 |
| MsG0480023088.01 | MsG0880047360.01 | -0.826512 | 2.604346e-54 | 2.447526e-51 |
| MsG0480023757.01 | MsG0880047360.01 | 0.800973 | 1.120889e-48 | 5.290779e-46 |
| MsG0580024938.01 | MsG0880047360.01 | -0.807792 | 4.251518e-50 | 2.389978e-47 |
| MsG0580025134.01 | MsG0880047360.01 | -0.821695 | 3.521764e-53 | 2.885994e-50 |
| MsG0380017135.01 | MsG0880047360.01 | 0.842922 | 1.923511e-58 | 2.960514e-55 |
| MsG0380017915.01 | MsG0880047360.01 | -0.813476 | 2.511208e-51 | 1.641641e-48 |
| MsG0380017998.01 | MsG0880047360.01 | -0.815521 | 8.859418e-52 | 6.122321e-49 |
| MsG0580029668.01 | MsG0880047360.01 | 0.853320 | 2.583548e-61 | 5.560305e-58 |
| MsG0280006475.01 | MsG0880047360.01 | 0.803724 | 3.041345e-49 | 1.539581e-46 |
| MsG0280006544.01 | MsG0880047360.01 | -0.863629 | 2.172679e-64 | 6.630696e-61 |
| MsG0280006595.01 | MsG0880047360.01 | -0.829985 | 3.789305e-55 | 3.940487e-52 |
| MsG0280006961.01 | MsG0880047360.01 | 0.817297 | 3.547850e-52 | 2.573704e-49 |
| MsG0280007133.01 | MsG0880047360.01 | 0.806342 | 8.617723e-50 | 4.665938e-47 |
| MsG0680035777.01 | MsG0880047360.01 | -0.848819 | 4.807801e-60 | 8.932896e-57 |
| MsG0780038645.01 | MsG0880047360.01 | -0.829623 | 4.640725e-55 | 4.775408e-52 |
| MsG0780039841.01 | MsG0880047360.01 | -0.836108 | 1.135061e-56 | 1.416338e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047360.01.T01 | MTR_4g125530 | 96.319 | 163 | 5 | 1 | 1 | 163 | 106 | 267 | 3.34e-114 | 325 |
| MsG0880047360.01.T01 | MTR_0121s0060 | 46.835 | 158 | 70 | 4 | 1 | 153 | 114 | 262 | 6.67e-42 | 140 |
| MsG0880047360.01.T01 | MTR_4g125560 | 44.720 | 161 | 78 | 5 | 1 | 158 | 64 | 216 | 1.84e-40 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047360.01.T01 | AT2G31670 | 54.194 | 155 | 68 | 2 | 1 | 153 | 102 | 255 | 1.02e-51 | 166 |
| MsG0880047360.01.T01 | AT1G51360 | 45.098 | 153 | 78 | 4 | 1 | 153 | 61 | 207 | 1.79e-35 | 123 |
Find 38 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCCACGTCAGCGTTGTTAA+AGG | 0.289711 | 8:-85253873 | MsG0880047360.01.T01:CDS |
| GGATCTTAACGGTGAGGATT+TGG | 0.330128 | 8:-85253799 | MsG0880047360.01.T01:CDS |
| TTTAACAACGCTGACGTGGC+TGG | 0.345995 | 8:+85253875 | None:intergenic |
| ATAAGCTTCGAGATCTTGTT+TGG | 0.384276 | 8:+85253908 | None:intergenic |
| GGAGAATGTTGTTAATGATA+AGG | 0.387273 | 8:-85253727 | MsG0880047360.01.T01:CDS |
| TTAACAACGCTGACGTGGCT+GGG | 0.404526 | 8:+85253876 | None:intergenic |
| AAAATTGACCAACTCATGAT+TGG | 0.404994 | 8:+85253554 | None:intergenic |
| ATGATATCATGGCCGTTGAT+TGG | 0.429475 | 8:-85253830 | MsG0880047360.01.T01:CDS |
| TTCTCTCCGGCGAGAGCTAA+AGG | 0.448262 | 8:-85253636 | MsG0880047360.01.T01:CDS |
| GATTGAGGAGGTGGCGGCGG+CGG | 0.452672 | 8:+85253474 | None:intergenic |
| TCCTATCATTGATGATATCA+TGG | 0.474974 | 8:-85253841 | MsG0880047360.01.T01:CDS |
| CAGGTTAAGCAAATTTCCTA+TGG | 0.478454 | 8:-85253666 | MsG0880047360.01.T01:CDS |
| AGTTATGTTCTTGAAATTGA+AGG | 0.483665 | 8:-85253751 | MsG0880047360.01.T01:CDS |
| CGGTGAGGATTTGGTTCCGA+AGG | 0.490577 | 8:-85253790 | MsG0880047360.01.T01:CDS |
| GATCAAAGACTTGCTGATTG+AGG | 0.494091 | 8:+85253459 | None:intergenic |
| TCCTCCATCTCCTTCTCTCC+CGG | 0.496909 | 8:+85253585 | None:intergenic |
| CTATGGTGAGAATTTCTCTC+CGG | 0.505194 | 8:-85253649 | MsG0880047360.01.T01:CDS |
| CTCATCGCAGAACCCTCCTT+CGG | 0.509845 | 8:+85253774 | None:intergenic |
| AGACTTGCTGATTGAGGAGG+TGG | 0.514147 | 8:+85253465 | None:intergenic |
| TGGATCGCTGAGGATCTTAA+CGG | 0.519445 | 8:-85253810 | MsG0880047360.01.T01:CDS |
| GCTGATTGAGGAGGTGGCGG+CGG | 0.519981 | 8:+85253471 | None:intergenic |
| GCCATGATATCATCAATGAT+AGG | 0.521154 | 8:+85253840 | None:intergenic |
| TTCCTTTAACAACGCTGACG+TGG | 0.528577 | 8:+85253871 | None:intergenic |
| TATGTTCTTGAAATTGAAGG+AGG | 0.534187 | 8:-85253748 | MsG0880047360.01.T01:CDS |
| GCTGACGTGGCTGGGGTGAG+CGG | 0.534569 | 8:+85253884 | None:intergenic |
| ATATTCGATTGCTTCGTTAG+CGG | 0.534946 | 8:-85253613 | MsG0880047360.01.T01:CDS |
| TGAGGATTTGGTTCCGAAGG+AGG | 0.542093 | 8:-85253787 | MsG0880047360.01.T01:CDS |
| CTTGCTGATTGAGGAGGTGG+CGG | 0.546516 | 8:+85253468 | None:intergenic |
| TAACAACGCTGACGTGGCTG+GGG | 0.552201 | 8:+85253877 | None:intergenic |
| CGGAGAGAAATTCTCACCAT+AGG | 0.569946 | 8:+85253650 | None:intergenic |
| GAGGATTTGGTTCCGAAGGA+GGG | 0.576392 | 8:-85253786 | MsG0880047360.01.T01:CDS |
| ACATGATTCCAATCATGAGT+TGG | 0.582044 | 8:-85253562 | MsG0880047360.01.T01:CDS |
| TCCGGGAGAGAAGGAGATGG+AGG | 0.592579 | 8:-85253586 | MsG0880047360.01.T01:CDS |
| ATCCTCAGCGATCCAATCAA+CGG | 0.598974 | 8:+85253818 | None:intergenic |
| GAATATCCTTTAGCTCTCGC+CGG | 0.608110 | 8:+85253630 | None:intergenic |
| CGCTGAGGATCTTAACGGTG+AGG | 0.620668 | 8:-85253805 | MsG0880047360.01.T01:CDS |
| GGCCGTTGATTGGATCGCTG+AGG | 0.675118 | 8:-85253820 | MsG0880047360.01.T01:CDS |
| CAAAGACTTGCTGATTGAGG+AGG | 0.689083 | 8:+85253462 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTTATGTTCTTGAAATTGA+AGG | - | Chr8:85253640-85253659 | MsG0880047360.01.T01:CDS | 25.0% |
| !! | AATTCCTGAAAATTTTCAAC+AGG | - | Chr8:85253706-85253725 | MsG0880047360.01.T01:CDS | 25.0% |
| !! | TGTTAATGATAAGGTTTTAC+AGG | - | Chr8:85253673-85253692 | MsG0880047360.01.T01:CDS | 25.0% |
| !! | TTAACCTGTTGAAAATTTTC+AGG | + | Chr8:85253713-85253732 | None:intergenic | 25.0% |
| AAAATTGACCAACTCATGAT+TGG | + | Chr8:85253840-85253859 | None:intergenic | 30.0% | |
| GGAGAATGTTGTTAATGATA+AGG | - | Chr8:85253664-85253683 | MsG0880047360.01.T01:CDS | 30.0% | |
| TATGTTCTTGAAATTGAAGG+AGG | - | Chr8:85253643-85253662 | MsG0880047360.01.T01:CDS | 30.0% | |
| ! | ATTTTCAGAAGGATAAGGTT+AGG | - | Chr8:85253855-85253874 | MsG0880047360.01.T01:CDS | 30.0% |
| ! | TCCTATCATTGATGATATCA+TGG | - | Chr8:85253550-85253569 | MsG0880047360.01.T01:CDS | 30.0% |
| ! | TTTTCAGAAGGATAAGGTTA+GGG | - | Chr8:85253856-85253875 | MsG0880047360.01.T01:CDS | 30.0% |
| ACATGATTCCAATCATGAGT+TGG | - | Chr8:85253829-85253848 | MsG0880047360.01.T01:CDS | 35.0% | |
| ATAAGCTTCGAGATCTTGTT+TGG | + | Chr8:85253486-85253505 | None:intergenic | 35.0% | |
| ATATTCGATTGCTTCGTTAG+CGG | - | Chr8:85253778-85253797 | MsG0880047360.01.T01:CDS | 35.0% | |
| CAGGTTAAGCAAATTTCCTA+TGG | - | Chr8:85253725-85253744 | MsG0880047360.01.T01:CDS | 35.0% | |
| GCCATGATATCATCAATGAT+AGG | + | Chr8:85253554-85253573 | None:intergenic | 35.0% | |
| ! | GGTCAATTTTCAGAAGGATA+AGG | - | Chr8:85253850-85253869 | MsG0880047360.01.T01:CDS | 35.0% |
| !!! | TGAGTTGGTCAATTTTCAGA+AGG | - | Chr8:85253844-85253863 | MsG0880047360.01.T01:CDS | 35.0% |
| ATGATATCATGGCCGTTGAT+TGG | - | Chr8:85253561-85253580 | MsG0880047360.01.T01:CDS | 40.0% | |
| CTATGGTGAGAATTTCTCTC+CGG | - | Chr8:85253742-85253761 | MsG0880047360.01.T01:CDS | 40.0% | |
| !!! | GTTTGGTTTTGTAACGAGTG+TGG | + | Chr8:85253469-85253488 | None:intergenic | 40.0% |
| ATCCTCAGCGATCCAATCAA+CGG | + | Chr8:85253576-85253595 | None:intergenic | 45.0% | |
| CGGAGAGAAATTCTCACCAT+AGG | + | Chr8:85253744-85253763 | None:intergenic | 45.0% | |
| GGATCTTAACGGTGAGGATT+TGG | - | Chr8:85253592-85253611 | MsG0880047360.01.T01:CDS | 45.0% | |
| TGGATCGCTGAGGATCTTAA+CGG | - | Chr8:85253581-85253600 | MsG0880047360.01.T01:CDS | 45.0% | |
| TTCCTTTAACAACGCTGACG+TGG | + | Chr8:85253523-85253542 | None:intergenic | 45.0% | |
| ! | TGCTTCGTTAGCGGTTTTTC+CGG | - | Chr8:85253787-85253806 | MsG0880047360.01.T01:CDS | 45.0% |
| !! | CAAAGACTTGCTGATTGAGG+AGG | + | Chr8:85253932-85253951 | None:intergenic | 45.0% |
| !! | GAATATCCTTTAGCTCTCGC+CGG | + | Chr8:85253764-85253783 | None:intergenic | 45.0% |
| AGCCACGTCAGCGTTGTTAA+AGG | - | Chr8:85253518-85253537 | MsG0880047360.01.T01:CDS | 50.0% | |
| TTAACAACGCTGACGTGGCT+GGG | + | Chr8:85253518-85253537 | None:intergenic | 50.0% | |
| TTTAACAACGCTGACGTGGC+TGG | + | Chr8:85253519-85253538 | None:intergenic | 50.0% | |
| ! | GAGGATTTGGTTCCGAAGGA+GGG | - | Chr8:85253605-85253624 | MsG0880047360.01.T01:CDS | 50.0% |
| ! | GCTTCGTTAGCGGTTTTTCC+GGG | - | Chr8:85253788-85253807 | MsG0880047360.01.T01:CDS | 50.0% |
| ! | TGAGGATTTGGTTCCGAAGG+AGG | - | Chr8:85253604-85253623 | MsG0880047360.01.T01:CDS | 50.0% |
| ! | TTTTCCGGGAGAGAAGGAGA+TGG | - | Chr8:85253802-85253821 | MsG0880047360.01.T01:CDS | 50.0% |
| !! | AGACTTGCTGATTGAGGAGG+TGG | + | Chr8:85253929-85253948 | None:intergenic | 50.0% |
| CGCTGAGGATCTTAACGGTG+AGG | - | Chr8:85253586-85253605 | MsG0880047360.01.T01:CDS | 55.0% | |
| CTCATCGCAGAACCCTCCTT+CGG | + | Chr8:85253620-85253639 | None:intergenic | 55.0% | |
| TAACAACGCTGACGTGGCTG+GGG | + | Chr8:85253517-85253536 | None:intergenic | 55.0% | |
| TCCTCCATCTCCTTCTCTCC+CGG | + | Chr8:85253809-85253828 | None:intergenic | 55.0% | |
| TTCTCTCCGGCGAGAGCTAA+AGG | - | Chr8:85253755-85253774 | MsG0880047360.01.T01:CDS | 55.0% | |
| ! | AGCGGTTTTTCCGGGAGAGA+AGG | - | Chr8:85253796-85253815 | MsG0880047360.01.T01:CDS | 55.0% |
| ! | CGGTGAGGATTTGGTTCCGA+AGG | - | Chr8:85253601-85253620 | MsG0880047360.01.T01:CDS | 55.0% |
| ! | CTTGCTGATTGAGGAGGTGG+CGG | + | Chr8:85253926-85253945 | None:intergenic | 55.0% |
| TCCGGGAGAGAAGGAGATGG+AGG | - | Chr8:85253805-85253824 | MsG0880047360.01.T01:CDS | 60.0% | |
| ! | GGCCGTTGATTGGATCGCTG+AGG | - | Chr8:85253571-85253590 | MsG0880047360.01.T01:CDS | 60.0% |
| ! | GCTGATTGAGGAGGTGGCGG+CGG | + | Chr8:85253923-85253942 | None:intergenic | 65.0% |
| GCTGACGTGGCTGGGGTGAG+CGG | + | Chr8:85253510-85253529 | None:intergenic | 70.0% | |
| ! | GATTGAGGAGGTGGCGGCGG+CGG | + | Chr8:85253920-85253939 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 85253461 | 85253952 | 85253461 | ID=MsG0880047360.01;Name=MsG0880047360.01 |
| Chr8 | mRNA | 85253461 | 85253952 | 85253461 | ID=MsG0880047360.01.T01;Parent=MsG0880047360.01;Name=MsG0880047360.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|163 |
| Chr8 | exon | 85253461 | 85253952 | 85253461 | ID=MsG0880047360.01.T01:exon:36377;Parent=MsG0880047360.01.T01 |
| Chr8 | CDS | 85253461 | 85253952 | 85253461 | ID=MsG0880047360.01.T01:cds;Parent=MsG0880047360.01.T01 |
| Gene Sequence |
| Protein sequence |
