AlfalfaGEDB | Msa0037760

M. sativa cultivar zhongmu-4 / Msa0037760

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010045 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071289 GO:0080090 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000026 GO:2000112 GO:2000280 GO:2001141
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037760	sp\|Q9SLD4\|ZAT11_ARATH	41.379	174	72	4	1	171	17	163	1.00e-30	113

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037760	A0A072VS27	94.211	190	9	1	1	188	16	205	3.05e-114	332

TFs/TRs:

Gene ID	Type	Classification
Msa0037760	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000820	Msa0037760	0.816207	6.227825e-52	-8.615850e-47
Msa0037760	Msa0151220	0.801382	9.246594e-49	-8.615850e-47
Msa0037760	Msa0264680	0.802767	4.798980e-49	-8.615850e-47
Msa0037760	Msa0279660	0.815876	7.385473e-52	-8.615850e-47
Msa0037760	Msa0302730	0.806873	6.656903e-50	-8.615850e-47
Msa0037760	Msa0345980	0.813989	1.935631e-51	-8.615850e-47
Msa0037760	Msa0367860	0.808438	3.096605e-50	-8.615850e-47
Msa0037760	Msa0393100	0.820661	6.096648e-53	-8.615850e-47
Msa0037760	Msa0484800	0.826648	2.417806e-54	-8.615850e-47
Msa0037760	Msa0488580	0.832098	1.147405e-55	-8.615850e-47
Msa0037760	Msa0534270	0.818710	1.700828e-52	-8.615850e-47
Msa0037760	Msa0592660	0.808697	2.726591e-50	-8.615850e-47
Msa0037760	Msa0615210	0.807770	4.298200e-50	-8.615850e-47
Msa0037760	Msa0791740	0.802976	4.345543e-49	-8.615850e-47
Msa0037760	Msa0863990	0.804662	1.939800e-49	-8.615850e-47
Msa0037760	Msa1025750	0.801613	8.289214e-49	-8.615850e-47
Msa0037760	Msa1034450	0.801784	7.645883e-49	-8.615850e-47
Msa0037760	Msa1061970	0.810067	1.385929e-50	-8.615850e-47
Msa0037760	Msa1074200	0.800508	1.394707e-48	-8.615850e-47
Msa0037760	Msa1114670	0.826804	2.218170e-54	-8.615850e-47
Msa0037760	Msa1120580	0.806162	9.403886e-50	-8.615850e-47
Msa0037760	Msa1157110	0.810236	1.274159e-50	-8.615850e-47
Msa0037760	Msa1159170	0.810937	8.990060e-51	-8.615850e-47
Msa0037760	Msa1221220	0.800514	1.390879e-48	-8.615850e-47
Msa0037760	Msa1225580	0.804447	2.150957e-49	-8.615850e-47
Msa0037760	Msa1355510	0.802720	4.907508e-49	-8.615850e-47
Msa0037760	Msa1413800	0.808580	2.887887e-50	-8.615850e-47
Msa0037760	Msa1436090	0.808260	3.379504e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037760	MtrunA17_Chr1g0212561	94.211	190	9	1	1	188	16	205	5.88e-118	332
Msa0037760	MtrunA17_Chr7g0222831	56.452	186	65	5	1	184	12	183	1.98e-50	160
Msa0037760	MtrunA17_Chr1g0182161	45.614	171	65	6	1	171	16	158	1.53e-34	120
Msa0037760	MtrunA17_Chr7g0263131	39.773	176	73	3	2	177	15	157	9.08e-33	115
Msa0037760	MtrunA17_Chr1g0182181	41.279	172	71	4	1	171	11	153	7.23e-32	112
Msa0037760	MtrunA17_Chr2g0327901	41.317	167	47	4	5	170	14	130	1.24e-28	103
Msa0037760	MtrunA17_Chr7g0263121	38.596	171	80	3	1	171	15	160	2.11e-28	104
Msa0037760	MtrunA17_Chr1g0197281	39.286	168	55	4	29	167	155	304	3.93e-24	97.1
Msa0037760	MtrunA17_Chr1g0180311	46.018	113	29	3	20	102	112	222	1.15e-20	87.0
Msa0037760	MtrunA17_Chr4g0027991	39.167	120	27	2	29	102	112	231	2.10e-19	83.6
Msa0037760	MtrunA17_Chr2g0320311	43.119	109	26	2	30	102	129	237	5.55e-19	82.4
Msa0037760	MtrunA17_Chr1g0188911	37.288	118	34	3	22	101	38	153	3.90e-15	70.1
Msa0037760	MtrunA17_Chr4g0012691	44.444	81	36	2	30	101	220	300	5.60e-14	68.9
Msa0037760	MtrunA17_Chr4g0012791	31.720	186	71	6	29	178	226	391	8.90e-14	68.6
Msa0037760	MtrunA17_Chr7g0231181	43.902	82	35	3	26	101	232	308	1.32e-13	67.8
Msa0037760	MtrunA17_Chr1g0207451	46.479	71	37	1	31	101	84	153	8.60e-13	64.7
Msa0037760	MtrunA17_Chr3g0134711	47.436	78	34	1	31	101	87	164	1.01e-11	61.6
Msa0037760	MtrunA17_Chr1g0188901	41.379	87	37	3	29	101	59	145	1.14e-11	60.8
Msa0037760	MtrunA17_Chr1g0152661	41.772	79	39	1	30	101	81	159	4.98e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037760	AT2G37430.1	41.379	174	72	4	1	171	17	163	1.02e-31	113
Msa0037760	AT5G59820.1	43.636	165	63	5	2	166	16	150	2.43e-29	106
Msa0037760	AT3G46080.1	51.145	131	39	5	2	125	16	128	2.86e-29	106
Msa0037760	AT2G28710.1	38.857	175	72	4	1	175	14	153	2.36e-28	103
Msa0037760	AT2G28200.1	35.683	227	83	8	1	183	77	284	1.41e-26	102
Msa0037760	AT3G46070.1	43.275	171	62	8	2	166	16	157	2.66e-26	99.4
Msa0037760	AT3G53600.1	40.268	149	58	3	23	171	43	160	3.22e-26	99.0
Msa0037760	AT3G46090.1	39.429	175	77	6	2	175	17	163	3.72e-26	98.6
Msa0037760	AT5G04390.1	47.525	101	26	2	29	102	152	252	8.52e-21	88.6
Msa0037760	AT5G03510.1	47.475	99	27	2	29	102	117	215	1.77e-20	86.7
Msa0037760	AT3G10470.1	46.154	104	26	3	29	102	181	284	8.46e-20	85.9
Msa0037760	AT5G43170.1	44.118	102	45	5	1	101	50	140	1.13e-16	74.7
Msa0037760	AT1G02030.1	48.101	79	33	2	27	101	157	231	1.23e-15	73.2
Msa0037760	AT2G45120.1	42.353	85	49	0	17	101	181	265	3.29e-15	72.4
Msa0037760	AT3G60580.1	44.737	76	36	2	30	101	173	246	8.96e-15	71.2
Msa0037760	AT1G49900.1	46.575	73	36	2	30	101	750	820	1.24e-14	71.6
Msa0037760	AT1G49900.1	44.872	78	36	3	29	101	192	267	3.69e-13	67.4
Msa0037760	AT3G19580.1	42.353	85	39	3	27	101	103	187	2.75e-14	69.7
Msa0037760	AT3G19580.2	42.353	85	39	3	27	101	103	187	2.75e-14	69.7
Msa0037760	AT5G67450.1	38.776	98	42	2	22	101	89	186	2.31e-13	66.6
Msa0037760	AT4G35280.1	39.831	118	53	4	30	132	162	276	7.04e-13	65.9
Msa0037760	AT2G17180.1	32.886	149	74	5	1	133	125	263	8.20e-13	65.5
Msa0037760	AT1G27730.1	38.532	109	60	2	30	131	80	188	6.16e-12	62.4
Msa0037760	AT5G04340.1	39.759	83	40	1	29	101	88	170	6.30e-12	62.8
Msa0037760	AT3G49930.1	36.449	107	58	3	5	101	65	171	1.00e-11	61.6
Msa0037760	AT5G56200.1	31.293	147	71	4	32	158	344	480	1.74e-11	62.4

Find 28 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCATTTGTGGATTAGAATT+TGG	0.159190	1_1:+66903873	Msa0037760:CDS
TGGACAACCAACTCTTAATT+TGG	0.245604	1_1:+66904148	Msa0037760:CDS
AAATACTTGATGTTACTTTC+TGG	0.260688	1_1:+66903636	Msa0037760:CDS
ATTTGGAATAGGACAAGCTT+TGG	0.376115	1_1:+66903890	Msa0037760:CDS
ACGCATGCATGTTCCATTTG+TGG	0.385337	1_1:+66903861	Msa0037760:CDS
TGTGGATTAGAATTTGGAAT+AGG	0.387282	1_1:+66903879	Msa0037760:CDS
TCAAAAGCTAATAATTCAAA+TGG	0.429533	1_1:+66903948	Msa0037760:CDS
TCCAAATTCTAATCCACAAA+TGG	0.431756	1_1:-66903874	None:intergenic
ACAACCAACTCTTAATTTGG+TGG	0.451155	1_1:+66904151	Msa0037760:CDS
TTTGGAATAGGACAAGCTTT+GGG	0.459019	1_1:+66903891	Msa0037760:CDS
TCACAAGAAACCAAGGTTGA+TGG	0.462801	1_1:+66903782	Msa0037760:CDS
AACTTCAAATCATTCTCTAA+AGG	0.507967	1_1:-66904116	None:intergenic
TACTTGATGTTACTTTCTGG+TGG	0.513625	1_1:+66903639	Msa0037760:CDS
ACTACTAGTAGTAGCAATAG+TGG	0.516156	1_1:+66903999	Msa0037760:CDS
CTAGTCATCTCCATCAACCT+TGG	0.544532	1_1:-66903792	None:intergenic
CAATCCACCAAATTAAGAGT+TGG	0.550722	1_1:-66904155	None:intergenic
TTGATGTTACTTTCTGGTGG+AGG	0.550811	1_1:+66903642	Msa0037760:CDS
TAACATCAAGTATTTAGCCA+TGG	0.579745	1_1:-66903628	None:intergenic
GATTCAGCAAAGAGGAGCAA+GGG	0.587011	1_1:+66904053	Msa0037760:CDS
AAGCTTTGGGTGGTCACATG+AGG	0.610856	1_1:+66903904	Msa0037760:CDS
TCATCGTTTCAAGCGTTGGG+AGG	0.614601	1_1:+66903747	Msa0037760:CDS
CTAATACTGATTCAGCAAAG+AGG	0.617636	1_1:+66904045	Msa0037760:CDS
TGATTCAGCAAAGAGGAGCA+AGG	0.619501	1_1:+66904052	Msa0037760:CDS
GGAATAGGACAAGCTTTGGG+TGG	0.625426	1_1:+66903894	Msa0037760:CDS
ATTCAGCAAAGAGGAGCAAG+GGG	0.654634	1_1:+66904054	Msa0037760:CDS
GTGCGAGTCACAAGAAACCA+AGG	0.659856	1_1:+66903775	Msa0037760:CDS
ATGTCTTGCACTCAAACACA+CGG	0.662690	1_1:-66903710	None:intergenic
ATGACTAGTGATGATCAACA+TGG	0.726427	1_1:+66903807	Msa0037760:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCAAAAGCTAATAATTCAAA+TGG	+	chr1_1:66903948-66903967	Msa0037760:CDS	20.0%
!!!	GAATGATTTGAAGTTTTTAA+AGG	+	chr1_1:66904124-66904143	Msa0037760:CDS	20.0%
!!!	AAATACTTGATGTTACTTTC+TGG	+	chr1_1:66903636-66903655	Msa0037760:CDS	25.0%
!	AACTTCAAATCATTCTCTAA+AGG	-	chr1_1:66904119-66904138	None:intergenic	25.0%
!!!	GATTTGAAGTTTTTAAAGGT+TGG	+	chr1_1:66904128-66904147	Msa0037760:CDS	25.0%
!!	TCCATTTGTGGATTAGAATT+TGG	+	chr1_1:66903873-66903892	Msa0037760:CDS	30.0%
	TCCAAATTCTAATCCACAAA+TGG	-	chr1_1:66903877-66903896	None:intergenic	30.0%
!!	TGTGGATTAGAATTTGGAAT+AGG	+	chr1_1:66903879-66903898	Msa0037760:CDS	30.0%
!!	TACTTGATGTTACTTTCTGG+TGG	+	chr1_1:66903639-66903658	Msa0037760:CDS	35.0%
!	TTTTCATCGTTTCAAGCGTT+GGG	+	chr1_1:66903744-66903763	Msa0037760:CDS	35.0%
	ATGACTAGTGATGATCAACA+TGG	+	chr1_1:66903807-66903826	Msa0037760:CDS	35.0%
!	ATTTGGAATAGGACAAGCTT+TGG	+	chr1_1:66903890-66903909	Msa0037760:CDS	35.0%
!	TTTGGAATAGGACAAGCTTT+GGG	+	chr1_1:66903891-66903910	Msa0037760:CDS	35.0%
	ACTACTAGTAGTAGCAATAG+TGG	+	chr1_1:66903999-66904018	Msa0037760:CDS	35.0%
!!	CTAATACTGATTCAGCAAAG+AGG	+	chr1_1:66904045-66904064	Msa0037760:CDS	35.0%
!!	CAACAAGAGACTTTTGTTTC+TGG	+	chr1_1:66904079-66904098	Msa0037760:CDS	35.0%
	TGGACAACCAACTCTTAATT+TGG	+	chr1_1:66904148-66904167	Msa0037760:CDS	35.0%
!	ACAACCAACTCTTAATTTGG+TGG	+	chr1_1:66904151-66904170	Msa0037760:CDS	35.0%
	CAATCCACCAAATTAAGAGT+TGG	-	chr1_1:66904158-66904177	None:intergenic	35.0%
!!	TTGATGTTACTTTCTGGTGG+AGG	+	chr1_1:66903642-66903661	Msa0037760:CDS	40.0%
	ATGTCTTGCACTCAAACACA+CGG	-	chr1_1:66903713-66903732	None:intergenic	40.0%
!	GTTTTCATCGTTTCAAGCGT+TGG	+	chr1_1:66903743-66903762	Msa0037760:CDS	40.0%
	TCACAAGAAACCAAGGTTGA+TGG	+	chr1_1:66903782-66903801	Msa0037760:CDS	40.0%
	CTAGTCATCTCCATCAACCT+TGG	-	chr1_1:66903795-66903814	None:intergenic	45.0%
!!	TGCGTTTTAGTCTTCGTCGT+TGG	-	chr1_1:66903846-66903865	None:intergenic	45.0%
	ACGCATGCATGTTCCATTTG+TGG	+	chr1_1:66903861-66903880	Msa0037760:CDS	45.0%
	TGATTCAGCAAAGAGGAGCA+AGG	+	chr1_1:66904052-66904071	Msa0037760:CDS	45.0%
	GATTCAGCAAAGAGGAGCAA+GGG	+	chr1_1:66904053-66904072	Msa0037760:CDS	45.0%
	ATTCAGCAAAGAGGAGCAAG+GGG	+	chr1_1:66904054-66904073	Msa0037760:CDS	45.0%
!!	TCATCGTTTCAAGCGTTGGG+AGG	+	chr1_1:66903747-66903766	Msa0037760:CDS	50.0%
	GTGCGAGTCACAAGAAACCA+AGG	+	chr1_1:66903775-66903794	Msa0037760:CDS	50.0%
!!	GGAATAGGACAAGCTTTGGG+TGG	+	chr1_1:66903894-66903913	Msa0037760:CDS	50.0%
!!	AAGCTTTGGGTGGTCACATG+AGG	+	chr1_1:66903904-66903923	Msa0037760:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	66903630	66904196	66903630	ID=Msa0037760;Name=Msa0037760
chr1_1	mRNA	66903630	66904196	66903630	ID=Msa0037760-mRNA-1;Parent=Msa0037760;Name=Msa0037760-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|188
chr1_1	exon	66903630	66904196	66903630	ID=Msa0037760-mRNA-1:exon:21660;Parent=Msa0037760-mRNA-1
chr1_1	CDS	66903630	66904196	66903630	ID=Msa0037760-mRNA-1:cds;Parent=Msa0037760-mRNA-1

Gene Sequence

>Msa0037760

ATGGCTAAATACTTGATGTTACTTTCTGGTGGAGGTGATAAAATATTTGATCAAGTGAATTACTCTTCAAATTTCAACAACCGTGTGTTTGAGTGCAAGACATGTAAAAGACAGTTTTCATCGTTTCAAGCGTTGGGAGGACATCGTGCGAGTCACAAGAAACCAAGGTTGATGGAGATGACTAGTGATGATCAACATGGTTCAATACTTACACCAACGACGAAGACTAAAACGCATGCATGTTCCATTTGTGGATTAGAATTTGGAATAGGACAAGCTTTGGGTGGTCACATGAGGCGACACAGAAGAACAGAATCATCAAAAGCTAATAATTCAAATGGAAACATGCATAATTTTATGACTACTACAACTACTAGTAGTAGCAATAGTGGTTGCAGTACTATTGATAATAGTACTAATACTGATTCAGCAAAGAGGAGCAAGGGGAACAACAAGAGACTTTTGTTTCTGGATTTGAATTTGACACCTTTAGAGAATGATTTGAAGTTTTTAAAGGTTGGACAACCAACTCTTAATTTGGTGGATTGTTTCAATTCAATTCATTAA

Protein sequence

>Msa0037760

MAKYLMLLSGGGDKIFDQVNYSSNFNNRVFECKTCKRQFSSFQALGGHRASHKKPRLMEMTSDDQHGSILTPTTKTKTHACSICGLEFGIGQALGGHMRRHRRTESSKANNSNGNMHNFMTTTTTSSSNSGCSTIDNSTNTDSAKRSKGNNKRLLFLDLNLTPLENDLKFLKVGQPTLNLVDCFNSIH*