AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0592660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592660 MtrunA17_Chr4g0010651 46.692 786 272 15 2 692 618 1351 0.0 612
Msa0592660 MtrunA17_Chr4g0010521 47.625 779 270 17 2 692 616 1344 0.0 578
Msa0592660 MtrunA17_Chr5g0442761 34.146 492 175 13 2 379 624 1080 1.04e-59 220
Msa0592660 MtrunA17_Chr6g0482111 29.091 440 214 13 1 360 624 1045 3.49e-40 160
Msa0592660 MtrunA17_Chr3g0081531 28.941 425 225 13 2 353 615 1035 1.38e-38 155
Msa0592660 MtrunA17_Chr3g0084151 26.636 428 222 9 5 346 612 1033 1.02e-33 139
Msa0592660 MtrunA17_Chr6g0480401 31.250 240 145 2 2 221 109 348 3.54e-25 108
Msa0592660 MtrunA17_Chr8g0371711 25.806 434 202 14 1 343 630 1034 1.58e-23 107
Msa0592660 MtrunA17_Chr6g0480521 25.126 398 245 12 1 353 611 1000 1.73e-23 107
Msa0592660 MtrunA17_Chr8g0353371 29.804 255 132 5 1 209 639 892 8.88e-22 101
Msa0592660 MtrunA17_Chr6g0480051 24.454 458 255 14 2 383 390 832 8.96e-22 101
Msa0592660 MtrunA17_Chr6g0481011 24.009 429 219 10 1 346 641 1045 2.73e-21 99.8
Msa0592660 MtrunA17_Chr6g0480911 24.883 426 222 13 1 343 637 1047 2.86e-21 99.8
Msa0592660 MtrunA17_Chr6g0479651 30.303 264 135 6 2 221 622 880 1.32e-20 97.8
Msa0592660 MtrunA17_Chr2g0300251 24.344 419 226 14 1 338 617 1025 5.20e-20 95.9
Msa0592660 MtrunA17_Chr6g0451551 26.835 395 235 11 1 343 773 1165 7.67e-20 95.1
Msa0592660 MtrunA17_Chr8g0354701 29.880 251 131 5 4 210 631 880 2.38e-19 94.0
Msa0592660 MtrunA17_Chr8g0354681 29.658 263 136 4 2 218 632 891 2.60e-19 93.6
Msa0592660 MtrunA17_Chr8g0354721 29.412 255 131 4 2 210 628 879 4.14e-19 93.2
Msa0592660 MtrunA17_Chr1g0146421 29.268 246 142 5 1 221 607 845 6.37e-19 92.0
Msa0592660 MtrunA17_Chr8g0354711 29.644 253 130 5 2 210 627 875 9.26e-19 92.0
Msa0592660 MtrunA17_Chr8g0368251 25.121 414 236 16 1 353 609 1009 1.25e-18 91.3
Msa0592660 MtrunA17_Chr6g0479631 28.395 243 150 5 1 221 613 853 2.13e-18 90.5
Msa0592660 MtrunA17_Chr6g0482511 31.274 259 134 7 1 218 655 910 3.16e-18 90.1
Msa0592660 MtrunA17_Chr6g0484161 24.819 415 238 12 1 350 633 1038 4.06e-18 89.7
Msa0592660 MtrunA17_Chr6g0480981 24.205 409 241 8 2 346 369 772 6.93e-18 88.6
Msa0592660 MtrunA17_Chr5g0417051 30.088 226 144 4 1 221 30 246 7.21e-18 84.7
Msa0592660 MtrunA17_Chr6g0479641 29.464 224 138 4 2 208 616 836 1.75e-17 87.4
Msa0592660 MtrunA17_Chr6g0478451 24.337 415 245 12 1 352 616 1024 2.30e-17 87.0
Msa0592660 MtrunA17_Chr6g0478741 29.707 239 152 6 1 231 599 829 2.50e-17 87.0
Msa0592660 MtrunA17_Chr6g0479051 26.552 290 142 6 1 221 577 864 3.20e-17 86.7
Msa0592660 MtrunA17_Chr4g0025751 29.412 238 123 7 1 208 597 819 3.63e-17 86.7
Msa0592660 MtrunA17_Chr4g0006771 28.571 266 135 5 1 218 613 871 5.28e-17 85.9
Msa0592660 MtrunA17_Chr2g0299841 27.368 190 93 2 2 150 664 849 2.07e-16 84.0
Msa0592660 MtrunA17_Chr2g0299851 22.955 379 184 11 2 328 622 944 5.35e-16 82.8
Msa0592660 MtrunA17_Chr2g0299921 23.387 372 191 11 2 328 621 943 5.48e-16 82.8
Msa0592660 MtrunA17_Chr1g0153511 28.936 235 137 8 1 212 579 806 7.71e-16 82.4
Msa0592660 MtrunA17_Chr6g0482141 32.258 217 106 8 1 212 658 838 8.48e-16 82.0
Msa0592660 MtrunA17_Chr6g0480451 27.236 246 147 6 1 221 673 911 8.90e-16 82.0
Msa0592660 MtrunA17_Chr6g0481141 25.915 328 195 9 2 288 665 985 1.06e-15 82.0
Msa0592660 MtrunA17_Chr4g0025771 27.236 246 144 6 1 221 68 303 1.07e-15 80.9
Msa0592660 MtrunA17_Chr6g0479001 28.163 245 151 6 1 221 597 840 1.79e-15 81.3
Msa0592660 MtrunA17_Chr5g0415831 28.571 259 135 7 8 221 4 257 2.09e-15 79.3
Msa0592660 MtrunA17_Chr4g0025831 23.472 409 237 16 1 350 610 1001 2.29e-15 80.9
Msa0592660 MtrunA17_Chr6g0480861 34.969 163 101 2 59 218 5 165 2.47e-15 74.7
Msa0592660 MtrunA17_Chr6g0480321 29.741 232 131 7 1 207 468 692 2.57e-15 80.5
Msa0592660 MtrunA17_Chr6g0480041 28.571 224 140 2 1 204 629 852 2.68e-15 80.5
Msa0592660 MtrunA17_Chr2g0330121 31.336 217 130 4 1 212 496 698 2.80e-15 80.5
Msa0592660 MtrunA17_Chr6g0479761 25.877 228 147 3 1 207 471 697 4.24e-15 79.3
Msa0592660 MtrunA17_Chr6g0479671 27.273 264 147 5 1 221 612 873 4.75e-15 79.7
Msa0592660 MtrunA17_Chr2g0299791 22.520 373 196 10 2 328 623 948 1.01e-14 79.0
Msa0592660 MtrunA17_Chr6g0480421 28.444 225 137 5 1 208 472 689 1.04e-14 78.6
Msa0592660 MtrunA17_Chr4g0020591 30.159 252 124 4 4 210 631 875 1.71e-14 77.8
Msa0592660 MtrunA17_Chr6g0478771 28.745 247 147 7 1 221 598 841 1.82e-14 77.8
Msa0592660 MtrunA17_Chr6g0480311 28.509 228 141 3 1 208 601 826 2.01e-14 77.8
Msa0592660 MtrunA17_Chr2g0299881 21.867 375 196 11 2 328 627 952 4.08e-14 76.6
Msa0592660 MtrunA17_Chr8g0363891 29.279 222 135 6 1 210 255 466 6.60e-14 75.9
Msa0592660 MtrunA17_Chr6g0481231 27.155 232 140 4 1 208 7 233 7.45e-14 74.7
Msa0592660 MtrunA17_Chr4g0005221 29.500 200 99 4 16 212 7 167 1.04e-13 74.7
Msa0592660 MtrunA17_Chr6g0481251 28.692 237 130 7 1 208 471 697 1.27e-13 75.1
Msa0592660 MtrunA17_Chr3g0084721 24.865 370 183 11 2 321 627 951 3.38e-13 73.6
Msa0592660 MtrunA17_Chr4g0025811 28.326 233 132 7 1 208 606 828 3.58e-13 73.6
Msa0592660 MtrunA17_Chr6g0479621 27.556 225 138 4 1 204 609 829 4.12e-13 73.6
Msa0592660 MtrunA17_Chr6g0482321 32.243 214 110 8 1 212 627 807 6.41e-13 72.8
Msa0592660 MtrunA17_Chr2g0299811 22.687 335 175 8 2 292 470 764 7.33e-13 72.4
Msa0592660 MtrunA17_Chr3g0084731 24.011 379 188 13 2 328 625 955 1.35e-12 71.6
Msa0592660 MtrunA17_Chr7g0248121 29.018 224 120 7 5 212 746 946 1.62e-12 71.6
Msa0592660 MtrunA17_Chr2g0319821 30.244 205 106 6 5 197 738 917 1.88e-12 71.2
Msa0592660 MtrunA17_Chr6g0479841 26.721 247 146 6 2 221 267 505 2.02e-12 70.9
Msa0592660 MtrunA17_Chr8g0342791 31.624 234 131 9 1 212 629 855 2.20e-12 71.2
Msa0592660 MtrunA17_Chr2g0319921 27.442 215 130 5 5 211 709 905 2.60e-12 70.9
Msa0592660 MtrunA17_Chr1g0172541 31.019 216 109 8 1 212 602 781 3.36e-12 70.5
Msa0592660 MtrunA17_Chr3g0084841 23.622 381 185 15 2 328 628 956 3.38e-12 70.5
Msa0592660 MtrunA17_Chr8g0363851 29.412 221 140 5 1 212 719 932 3.54e-12 70.5
Msa0592660 MtrunA17_Chr4g0025741 27.511 229 131 7 1 204 608 826 5.64e-12 69.7
Msa0592660 MtrunA17_Chr7g0242801 28.061 196 112 4 5 200 775 941 5.92e-12 69.7
Msa0592660 MtrunA17_Chr2g0319851 29.167 192 108 4 5 196 734 897 6.87e-12 69.3
Msa0592660 MtrunA17_Chr6g0479821 25.000 224 128 5 1 221 620 806 7.98e-12 69.3
Msa0592660 MtrunA17_Chr6g0481191 31.138 167 97 7 1 161 232 386 1.44e-11 67.4
Msa0592660 MtrunA17_Chr6g0480461 29.319 191 116 7 2 185 659 837 2.97e-11 67.4
Msa0592660 MtrunA17_Chr6g0478841 26.840 231 142 7 1 208 598 824 3.39e-11 67.0
Msa0592660 MtrunA17_Chr6g0486221 27.074 229 110 6 4 197 634 840 3.68e-11 67.0
Msa0592660 MtrunA17_Chr8g0342641 28.571 259 137 8 1 215 610 864 4.03e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592660 AT1G69550.1 30.345 290 145 9 4 246 981 1260 3.28e-19 94.0
Msa0592660 AT1G69550.1 33.632 223 127 7 4 217 909 1119 7.13e-18 89.4
Msa0592660 AT3G25510.2 31.250 224 133 7 2 217 459 669 2.64e-17 87.4
Msa0592660 AT3G25510.2 29.707 239 134 8 1 212 576 807 2.19e-13 74.7
Msa0592660 AT3G25510.2 27.386 241 132 6 8 210 678 913 1.64e-11 68.6
Msa0592660 AT3G25510.1 31.250 224 133 7 2 217 459 669 2.64e-17 87.4
Msa0592660 AT3G25510.1 29.707 239 134 8 1 212 576 807 2.19e-13 74.7
Msa0592660 AT3G25510.1 27.386 241 132 6 8 210 678 913 1.64e-11 68.6
Msa0592660 AT2G16870.1 34.742 213 112 7 4 210 624 815 4.47e-17 86.7
Msa0592660 AT2G16870.3 34.742 213 112 7 4 210 350 541 4.48e-17 86.7
Msa0592660 AT2G16870.2 34.742 213 112 7 4 210 350 541 4.48e-17 86.7
Msa0592660 AT5G18350.1 29.032 217 128 5 2 203 644 849 8.34e-17 85.9
Msa0592660 AT5G44510.3 33.058 242 130 7 2 217 656 891 5.18e-16 83.2
Msa0592660 AT5G44510.3 32.489 237 120 11 8 213 757 984 4.68e-13 73.6
Msa0592660 AT5G44510.2 33.058 242 130 7 2 217 656 891 5.71e-16 83.2
Msa0592660 AT5G44510.2 32.489 237 120 11 8 213 757 984 4.82e-13 73.6
Msa0592660 AT5G44510.1 33.058 242 130 7 2 217 656 891 5.74e-16 83.2
Msa0592660 AT5G44510.1 32.489 237 120 11 8 213 757 984 5.18e-13 73.6
Msa0592660 AT5G44510.4 33.058 242 130 7 2 217 656 891 6.64e-16 82.8
Msa0592660 AT5G44510.4 32.489 237 120 11 8 213 757 984 6.11e-13 73.2
Msa0592660 AT5G45060.2 30.275 218 132 6 1 217 681 879 2.18e-15 81.3
Msa0592660 AT5G45060.1 30.275 218 132 6 1 217 681 879 2.18e-15 81.3
Msa0592660 AT5G17880.2 28.959 221 138 6 5 224 709 911 3.34e-15 80.9
Msa0592660 AT5G17880.1 28.959 221 138 6 5 224 709 911 4.04e-15 80.5
Msa0592660 AT4G19530.1 29.183 257 154 8 5 252 714 951 4.47e-15 80.1
Msa0592660 AT1G63750.3 27.727 220 137 4 2 217 629 830 5.42e-15 80.1
Msa0592660 AT1G63750.1 28.054 221 135 5 2 217 462 663 6.13e-15 79.7
Msa0592660 AT1G63750.2 28.054 221 135 5 2 217 462 663 6.13e-15 79.7
Msa0592660 AT5G17680.1 28.302 265 133 7 2 224 624 873 8.37e-15 79.3
Msa0592660 AT5G17680.2 28.302 265 133 7 2 224 625 874 8.66e-15 79.3
Msa0592660 AT3G44480.2 31.472 197 116 4 1 193 759 940 1.22e-14 79.0
Msa0592660 AT3G44480.4 31.472 197 116 4 1 193 759 940 1.36e-14 78.6
Msa0592660 AT3G44480.1 31.472 197 116 4 1 193 759 940 1.36e-14 78.6
Msa0592660 AT3G44480.3 31.472 197 116 4 1 193 759 940 1.51e-14 78.6
Msa0592660 AT3G44480.5 31.472 197 116 4 1 193 759 940 1.51e-14 78.6
Msa0592660 AT2G14080.2 31.313 198 118 4 2 193 540 725 2.09e-14 78.2
Msa0592660 AT2G14080.1 31.313 198 118 4 2 193 673 858 2.32e-14 78.2
Msa0592660 AT5G45200.1 29.437 231 135 5 2 212 708 930 2.67e-14 77.8
Msa0592660 AT4G19530.2 30.698 215 129 5 5 217 714 910 2.90e-14 77.8
Msa0592660 AT5G45200.3 29.437 231 135 5 2 212 708 930 3.70e-14 77.4
Msa0592660 AT5G45200.2 29.825 228 132 5 3 210 465 684 3.99e-14 77.0
Msa0592660 AT5G38850.1 31.754 211 125 5 4 212 615 808 8.01e-14 76.3
Msa0592660 AT5G38350.1 27.751 209 132 5 4 210 479 670 1.47e-13 75.1
Msa0592660 AT5G38340.3 27.928 222 139 4 4 210 680 895 2.17e-13 74.7
Msa0592660 AT5G38340.1 27.928 222 139 4 4 210 680 895 2.17e-13 74.7
Msa0592660 AT5G38340.2 27.928 222 139 4 4 210 474 689 2.19e-13 74.7
Msa0592660 AT4G36150.1 28.346 254 132 9 1 230 697 924 2.42e-13 74.7
Msa0592660 AT5G11250.3 25.694 288 153 7 4 246 713 984 3.22e-13 74.3
Msa0592660 AT5G11250.1 25.694 288 153 7 4 246 713 984 3.22e-13 74.3
Msa0592660 AT5G11250.2 25.694 288 153 7 4 246 795 1066 3.35e-13 74.3
Msa0592660 AT5G41750.1 31.429 210 121 5 4 210 627 816 5.67e-13 73.6
Msa0592660 AT5G41750.2 31.429 210 121 5 4 210 627 816 5.67e-13 73.6
Msa0592660 AT3G44630.2 25.610 246 138 5 4 210 787 1026 5.98e-13 73.6
Msa0592660 AT3G44630.1 25.610 246 138 5 4 210 787 1026 5.98e-13 73.6
Msa0592660 AT3G44630.4 25.610 246 138 5 4 210 787 1026 5.98e-13 73.6
Msa0592660 AT3G44630.3 25.610 246 138 5 4 210 787 1026 7.45e-13 73.2
Msa0592660 AT3G04220.1 29.231 195 120 4 2 190 667 849 7.78e-13 72.8
Msa0592660 AT3G04220.3 29.231 195 120 4 2 190 696 878 8.26e-13 72.8
Msa0592660 AT5G45250.1 28.638 213 132 5 1 212 705 898 9.17e-13 72.8
Msa0592660 AT1G27180.3 30.412 194 123 3 3 194 681 864 1.03e-12 72.4
Msa0592660 AT1G27180.2 30.412 194 123 3 3 194 681 864 1.13e-12 72.4
Msa0592660 AT1G27180.1 30.412 194 123 3 3 194 843 1026 1.16e-12 72.4
Msa0592660 AT5G41540.1 30.144 209 127 6 4 210 627 818 1.43e-12 72.0
Msa0592660 AT1G27180.7 30.412 194 123 3 3 194 505 688 1.50e-12 72.0
Msa0592660 AT1G27180.6 30.412 194 123 3 3 194 505 688 1.50e-12 72.0
Msa0592660 AT1G27180.4 30.412 194 123 3 3 194 505 688 1.50e-12 72.0
Msa0592660 AT1G27180.5 30.412 194 123 3 3 194 679 862 1.60e-12 72.0
Msa0592660 AT1G27180.8 30.412 194 123 3 3 194 679 862 1.60e-12 72.0
Msa0592660 AT3G51570.2 27.982 218 139 5 1 217 675 875 4.09e-12 70.9
Msa0592660 AT1G65850.3 27.193 228 133 5 4 210 547 762 5.32e-12 70.1
Msa0592660 AT3G51570.1 27.982 218 139 5 1 217 675 875 5.37e-12 70.5
Msa0592660 AT1G65850.2 27.193 228 133 5 4 210 672 887 5.51e-12 70.1
Msa0592660 AT1G65850.1 27.193 228 133 5 4 210 657 872 6.39e-12 70.1
Msa0592660 AT1G56540.1 27.962 211 130 6 4 210 627 819 7.53e-12 69.7
Msa0592660 AT1G56540.2 27.962 211 130 6 4 210 463 655 1.11e-11 69.3
Msa0592660 AT5G45230.1 26.000 250 162 7 4 247 706 938 1.22e-11 69.3
Msa0592660 AT1G63880.1 27.650 217 127 7 2 212 628 820 4.08e-11 67.4
Msa0592660 AT4G11170.1 25.843 267 157 10 2 252 630 871 9.17e-11 66.2

Find 171 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGAACCTTACCACTTTA+TGG 0.075575 4_2:-18154922 None:intergenic
AAAGATCCTTTGATGGATTT+TGG 0.100574 4_2:-18155460 None:intergenic
TCTTGAAAGTTTCCCGAATT+TGG 0.150668 4_2:+18155399 Msa0592660:CDS
CAATACTGGATTGGGAAATT+TGG 0.165203 4_2:+18157219 Msa0592660:CDS
TTGCTAAGGTTGAAAATAAA+TGG 0.187608 4_2:+18156534 Msa0592660:CDS
GTAAGATTACCAATGGAATT+TGG 0.191591 4_2:-18155151 None:intergenic
AATTTCAAGAATACACATTA+TGG 0.206331 4_2:+18157307 Msa0592660:CDS
ACGAGCTTTAATAAATCTTA+TGG 0.214525 4_2:+18157097 Msa0592660:CDS
AATTCCATTGGTAATCTTAC+AGG 0.216786 4_2:+18155154 Msa0592660:CDS
CCGACGCATGTCTCATTCTT+TGG 0.238431 4_2:-18156412 None:intergenic
TCTTAGAGGACAAGAAAATT+TGG 0.246240 4_2:+18157189 Msa0592660:CDS
AACTTAGAAAATCATTTAAA+AGG 0.247354 4_2:+18155281 Msa0592660:CDS
ATGTTGGGATCTGGGATTTC+AGG 0.256308 4_2:+18157265 Msa0592660:CDS
CTGGAAACTTTATTTCTTAG+AGG 0.261829 4_2:+18157175 Msa0592660:CDS
GTCCTTGAAGCATGTCATTT+TGG 0.262102 4_2:+18156797 Msa0592660:CDS
AAGCTTCAGCCATTGCCTTT+GGG 0.264624 4_2:+18156558 Msa0592660:CDS
ATTCTTAATGTGGAAAGTTT+TGG 0.268323 4_2:-18155244 None:intergenic
GAAGCTTCAGCCATTGCCTT+TGG 0.286259 4_2:+18156557 Msa0592660:CDS
ACCACCATGGGAATTAATAT+TGG 0.288928 4_2:-18156324 None:intergenic
TTGCTTTAGCATTGGTGTTT+GGG 0.295925 4_2:+18155949 Msa0592660:CDS
AATTTGGAAATGGGATTGTT+AGG 0.296873 4_2:+18157235 Msa0592660:CDS
CACATCAGGTATTTGAGTTA+TGG 0.300766 4_2:-18154858 None:intergenic
GAGTTATTGCAGGAGTCTTA+TGG 0.306610 4_2:+18155504 Msa0592660:CDS
TCAATGCTTAATGGAAGTTC+TGG 0.307483 4_2:-18155532 None:intergenic
GGATTAGGAATGTTGGGATC+TGG 0.310184 4_2:+18157256 Msa0592660:CDS
AATTTGGCACAAGGCAATAC+TGG 0.313254 4_2:+18157205 Msa0592660:CDS
TCGGTATCTCGTTATTCTGT+TGG 0.316307 4_2:-18155977 None:intergenic
GTGGAATTTATGAGGGAAAA+AGG 0.319254 4_2:+18156743 Msa0592660:CDS
ACATTCAAGTCTTCCAAATT+CGG 0.321387 4_2:-18155412 None:intergenic
ATCGTTGACTTTGAAATGTT+GGG 0.321746 4_2:+18156241 Msa0592660:CDS
AGATGATTGGAAGGCTGTTC+AGG 0.321750 4_2:+18156205 Msa0592660:CDS
GTTGCTTTAGCATTGGTGTT+TGG 0.321929 4_2:+18155948 Msa0592660:CDS
AGCTAAGAGTCGTCGCTATT+GGG 0.328983 4_2:+18156823 Msa0592660:CDS
ATGAATCCAAGAGAAATATT+TGG 0.330259 4_2:+18156440 Msa0592660:CDS
AATTCTTGCGACATACTTCT+TGG 0.332012 4_2:-18156082 None:intergenic
CTTTCCACATTAAGAATTGA+TGG 0.334420 4_2:+18155250 Msa0592660:CDS
CGAGCTTTAATAAATCTTAT+GGG 0.340444 4_2:+18157098 Msa0592660:CDS
AGTTTCTGACAAATATTTGC+TGG 0.342263 4_2:+18157156 Msa0592660:CDS
GAAATGGGATTGTTAGGATT+AGG 0.353659 4_2:+18157241 Msa0592660:CDS
GGATCTGGGATTTCAGGATT+TGG 0.354053 4_2:+18157271 Msa0592660:CDS
AAGCTAAGAGTCGTCGCTAT+TGG 0.356701 4_2:+18156822 Msa0592660:CDS
CATCGTTGACTTTGAAATGT+TGG 0.357134 4_2:+18156240 Msa0592660:CDS
TTTAACACCGCTTTGAACTT+TGG 0.357217 4_2:-18156872 None:intergenic
TACTCACCAAATATTTCTCT+TGG 0.357465 4_2:-18156446 None:intergenic
CAAATGCAAAGATCCTTTGA+TGG 0.358884 4_2:-18155467 None:intergenic
CGGTATCTCGTTATTCTGTT+GGG 0.360923 4_2:-18155976 None:intergenic
TTCATCTCCGACTTCTTCCC+TGG 0.363178 4_2:+18156008 Msa0592660:CDS
TGGATTGGGAAATTTGGAAA+TGG 0.366025 4_2:+18157225 Msa0592660:CDS
TTGTTAGGATTAGGAATGTT+GGG 0.371661 4_2:+18157250 Msa0592660:CDS
TCATCTCCAACATAAGTATC+TGG 0.372887 4_2:-18156188 None:intergenic
GCCAATGTCGGCATCAGGTT+TGG 0.373520 4_2:+18156361 Msa0592660:CDS
ATTGTTAGGATTAGGAATGT+TGG 0.375443 4_2:+18157249 Msa0592660:CDS
ATTACCATTACTGCAGTGTT+GGG 0.376638 4_2:+18156102 Msa0592660:CDS
CATTCAAGTCTTCCAAATTC+GGG 0.376833 4_2:-18155411 None:intergenic
GATTAGGAATGTTGGGATCT+GGG 0.384673 4_2:+18157257 Msa0592660:CDS
AAGCAAGAGCATGAAGAATC+TGG 0.387024 4_2:+18156590 Msa0592660:CDS
AACAATGCCTTTGTAAAACT+TGG 0.391421 4_2:-18156497 None:intergenic
ATCATCAACTCTTCCAAAAC+AGG 0.391933 4_2:-18157055 None:intergenic
ATACTTATGTTGGAGATGAT+TGG 0.392084 4_2:+18156192 Msa0592660:CDS
AGCAAATGGCAGCTCAAGTT+TGG 0.393228 4_2:+18155324 Msa0592660:CDS
ATTTCAAGAATACACATTAT+GGG 0.394045 4_2:+18157308 Msa0592660:CDS
TGACATGCTTCAAGGACAAT+AGG 0.400152 4_2:-18156791 None:intergenic
CCCTATGGTTGCTTTAGCAT+TGG 0.403694 4_2:+18155941 Msa0592660:CDS
AGAGTACTATGATCCTGTTT+TGG 0.407150 4_2:+18157042 Msa0592660:CDS
GATAACATTCCTAAACATAT+TGG 0.412468 4_2:+18156647 Msa0592660:CDS
GGATTGGGAAATTTGGAAAT+GGG 0.415147 4_2:+18157226 Msa0592660:CDS
CAAAAGAAGTCTCAAATGAT+AGG 0.415620 4_2:+18156930 Msa0592660:CDS
GCACAAGGCAATACTGGATT+GGG 0.417455 4_2:+18157211 Msa0592660:CDS
GTCTTGACGTGAGTTATTGC+AGG 0.420282 4_2:+18155494 Msa0592660:CDS
TACGAATCACCAATATGTTT+AGG 0.421101 4_2:-18156656 None:intergenic
CCATTGCCAATGTCGGCATC+AGG 0.425895 4_2:+18156356 Msa0592660:CDS
TTACTATTCACAGAAATTTG+AGG 0.426839 4_2:+18154802 None:intergenic
CACCAAAATGACATGCTTCA+AGG 0.427260 4_2:-18156799 None:intergenic
GGTCTGCATATATTTATTGA+TGG 0.428191 4_2:+18156059 Msa0592660:CDS
CCACCATGGGAATTAATATT+GGG 0.428832 4_2:-18156323 None:intergenic
TGCTAAGGTTGAAAATAAAT+GGG 0.432454 4_2:+18156535 Msa0592660:CDS
GGGCAAGGGTGGAATTTATG+AGG 0.443719 4_2:+18156735 Msa0592660:CDS
CTTACTCTTACTGTGAATAA+TGG 0.447315 4_2:-18157560 None:intergenic
CAAAGGCATCAAGCATTCTA+TGG 0.448512 4_2:+18155590 Msa0592660:CDS
CTTCCTCTGTAGATGGTCTC+TGG 0.450693 4_2:-18156976 None:intergenic
GGCACAAGGCAATACTGGAT+TGG 0.451284 4_2:+18157210 Msa0592660:CDS
GTTGAAGCTTCCTCTGTAGA+TGG 0.453203 4_2:-18156983 None:intergenic
CAAAGGCATTGTTGAGGTCG+TGG 0.455935 4_2:+18156507 Msa0592660:CDS
CAGCAACAATGAAGAGTGGC+AGG 0.469386 4_2:+18156163 Msa0592660:CDS
AGAGTTGATGATCATGATAG+AGG 0.471247 4_2:+18157066 Msa0592660:CDS
ATGTCTTAATGTGTGACCTT+AGG 0.471733 4_2:+18156132 Msa0592660:CDS
TTTCCCCAACACTGCAGTAA+TGG 0.473373 4_2:-18156106 None:intergenic
TGATAGGTACTGCTGTTAAG+AGG 0.473501 4_2:+18155125 Msa0592660:intron
ACTCAAATACCTGATGTGTC+TGG 0.480582 4_2:+18154863 Msa0592660:CDS
ACAGTAAGAGTAAGCAGGGT+TGG 0.483186 4_2:+18157569 Msa0592660:CDS
TATTCACAGTAAGAGTAAGC+AGG 0.491532 4_2:+18157564 Msa0592660:CDS
TTTATAGCTCCAGACACATC+AGG 0.492543 4_2:-18154872 None:intergenic
AAACATATTGGTGATTCGTA+TGG 0.494500 4_2:+18156659 Msa0592660:CDS
AATTACCATTACTGCAGTGT+TGG 0.496143 4_2:+18156101 Msa0592660:CDS
GTTTCCTCTAATTCTAAAAG+AGG 0.497546 4_2:-18156473 None:intergenic
TCGGAGATGAATGTGTCTTT+CGG 0.499694 4_2:-18155996 None:intergenic
GACATATCTGACATCAGTCC+AGG 0.500185 4_2:-18156026 None:intergenic
TGGAAGTAAGAAGAAGCTAC+TGG 0.503493 4_2:-18155224 None:intergenic
GAGAGCAATTCGGTAGCAAA+TGG 0.505166 4_2:+18155310 Msa0592660:CDS
ACCAAACCTGATGCCGACAT+TGG 0.511704 4_2:-18156362 None:intergenic
TGAAATGCAGCATGAGTTGA+GGG 0.514275 4_2:+18157528 Msa0592660:CDS
GGCTCGCCGGAAGTTCCCTA+TGG 0.514864 4_2:+18155926 Msa0592660:intron
TCAGATATGTCACACATTGT+TGG 0.515362 4_2:+18156038 Msa0592660:CDS
GTAAGGTTCAATGAATCCAT+TGG 0.515475 4_2:+18154932 Msa0592660:CDS
ATTTCAGGATTTGGAAGGAT+GGG 0.516158 4_2:+18157280 Msa0592660:CDS
CTGGGATTTCAGGATTTGGA+AGG 0.518849 4_2:+18157275 Msa0592660:CDS
TTGTGAAAGCTTAACTTCAA+AGG 0.519468 4_2:+18155573 Msa0592660:CDS
AAGGTTCAAAGAGAGCAATT+CGG 0.520572 4_2:+18155300 Msa0592660:CDS
AAGAGGTTTCCAAATTCCAT+TGG 0.521287 4_2:+18155142 Msa0592660:CDS
CTATGAGGAAGAATCCTTTG+AGG 0.523354 4_2:+18157018 Msa0592660:CDS
TCGAATGCAGCATGAGTTGA+GGG 0.525674 4_2:+18157429 Msa0592660:CDS
AACTTCAAGCTCTAGCTATG+AGG 0.528372 4_2:+18157003 Msa0592660:CDS
ACAAGACAAGCCAACTTGCA+TGG 0.530805 4_2:+18156894 Msa0592660:CDS
TTTCCACATTAAGAATTGAT+GGG 0.531667 4_2:+18155251 Msa0592660:CDS
CTTCCCAATATTAATTCCCA+TGG 0.535584 4_2:+18156320 Msa0592660:CDS
CAGGGTCCAGATACTTATGT+TGG 0.544888 4_2:+18156182 Msa0592660:CDS
ACAAAGTGCACAACGAATTG+TGG 0.545273 4_2:+18156700 Msa0592660:CDS
TGCTTTAGCATTGGTGTTTG+GGG 0.546892 4_2:+18155950 Msa0592660:CDS
ATCGAATGCAGCATGAGTTG+AGG 0.547240 4_2:+18157428 Msa0592660:CDS
GGCAAGGGTGGAATTTATGA+GGG 0.555203 4_2:+18156736 Msa0592660:CDS
AGCTTCAGCCATTGCCTTTG+GGG 0.555759 4_2:+18156559 Msa0592660:CDS
TCGTTGACTTTGAAATGTTG+GGG 0.555960 4_2:+18156242 Msa0592660:CDS
ATTCCATTGGTAATCTTACA+GGG 0.562794 4_2:+18155155 Msa0592660:CDS
TCAAGGCTTTCCATGCAAGT+TGG 0.563882 4_2:-18156904 None:intergenic
ATGAAATGCAGCATGAGTTG+AGG 0.564602 4_2:+18157527 Msa0592660:CDS
ATCAAGCATTCTATGGTCCA+AGG 0.567017 4_2:+18155597 Msa0592660:CDS
ACAGATGCCATAAAGTGGTA+AGG 0.569803 4_2:+18154915 Msa0592660:CDS
AAGAGCATGAAGAATCTGGA+TGG 0.570440 4_2:+18156594 Msa0592660:CDS
ACTTGACAGATGCCATAAAG+TGG 0.570631 4_2:+18154910 Msa0592660:CDS
AAGCCCTGTAAGATTACCAA+TGG 0.578635 4_2:-18155158 None:intergenic
ACCAATGCTAAAGCAACCAT+AGG 0.584043 4_2:-18155942 None:intergenic
CCTGATGCCGACATTGGCAA+TGG 0.585115 4_2:-18156356 None:intergenic
ATACACATTATGGGAAGTTG+AGG 0.585431 4_2:+18157317 Msa0592660:CDS
GCTAAGAGTCGTCGCTATTG+GGG 0.588862 4_2:+18156824 Msa0592660:CDS
GATTTCAGGATTTGGAAGGA+TGG 0.588893 4_2:+18157279 Msa0592660:CDS
GCGAGTTGTAGAGTTGATGA+AGG 0.589055 4_2:+18156625 Msa0592660:CDS
ATTCACAGTAAGAGTAAGCA+GGG 0.590162 4_2:+18157565 Msa0592660:CDS
TGTTGCTGAAGAAAGTCCTA+AGG 0.591312 4_2:-18156148 None:intergenic
ACACCCATCAATTCTTAATG+TGG 0.594371 4_2:-18155254 None:intergenic
GAGTAAGCAGGGTTGGATCA+CGG 0.594645 4_2:+18157576 Msa0592660:CDS
TGGCACATAGTCACCACCAT+GGG 0.595020 4_2:-18156336 None:intergenic
CCCAATATTAATTCCCATGG+TGG 0.596213 4_2:+18156323 Msa0592660:CDS
AACAGAAAGAGTCAAGGAGG+AGG 0.600592 4_2:+18157374 Msa0592660:CDS
ATCATAGTACTCTTCCTCAA+AGG 0.602768 4_2:-18157032 None:intergenic
TACACATTATGGGAAGTTGA+GGG 0.605027 4_2:+18157318 Msa0592660:CDS
CTCAGAAACAGAAAGAGTCA+AGG 0.606070 4_2:+18157368 Msa0592660:CDS
TCTCTCCCAAAATCCATCAA+AGG 0.608596 4_2:+18155454 Msa0592660:CDS
GTTGATGATCATGATAGAGG+AGG 0.610450 4_2:+18157069 Msa0592660:CDS
AGAGCATGAAGAATCTGGAT+GGG 0.610832 4_2:+18156595 Msa0592660:CDS
GCTTCAGCCATTGCCTTTGG+GGG 0.612360 4_2:+18156560 Msa0592660:CDS
ATTCTTTGGACAGACATCTG+AGG 0.615500 4_2:-18156398 None:intergenic
TTACCATTACTGCAGTGTTG+GGG 0.618708 4_2:+18156103 Msa0592660:CDS
CCAATGCTAAAGCAACCATA+GGG 0.631159 4_2:-18155941 None:intergenic
CATCAGTCCAGGGAAGAAGT+CGG 0.635445 4_2:-18156015 None:intergenic
CTATGTGCCATTGCCAATGT+CGG 0.636086 4_2:+18156349 Msa0592660:CDS
GAGGTCGTGGAGGATTGCTA+AGG 0.639453 4_2:+18156520 Msa0592660:CDS
TTATGTTGGAGATGATTGGA+AGG 0.643990 4_2:+18156196 Msa0592660:CDS
TCTTCAGCAACAATGAAGAG+TGG 0.644002 4_2:+18156159 Msa0592660:CDS
TTACTGTGAATAATGGTCTG+AGG 0.648617 4_2:-18157553 None:intergenic
AGCAACAATGAAGAGTGGCA+GGG 0.648844 4_2:+18156164 Msa0592660:CDS
ACATATCTGACATCAGTCCA+GGG 0.653601 4_2:-18156025 None:intergenic
GGACAAGAAAATTTGGCACA+AGG 0.656577 4_2:+18157196 Msa0592660:CDS
AGGCATTGTTGAGGTCGTGG+AGG 0.666833 4_2:+18156510 Msa0592660:CDS
TCAGACACAGAAAGAGTCAA+CGG 0.671368 4_2:+18157468 Msa0592660:CDS
AATGCAGCATGAGTTGAGGG+TGG 0.671604 4_2:+18157432 Msa0592660:CDS
CCAAAGAATGAGACATGCGT+CGG 0.682111 4_2:+18156412 Msa0592660:CDS
AGAAACAGAAAGAGTCAAGG+AGG 0.697107 4_2:+18157371 Msa0592660:CDS
ATGCCAGAGACCATCTACAG+AGG 0.701073 4_2:+18156973 Msa0592660:CDS
CGAAGATCCAAAGTTCAAAG+CGG 0.702576 4_2:+18156865 Msa0592660:CDS
ATGGCACATAGTCACCACCA+TGG 0.714158 4_2:-18156337 None:intergenic
ACATTATGGGAAGTTGAGGG+CGG 0.716577 4_2:+18157321 Msa0592660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTAATGTTATTTTATAATT+TGG + chr4_2:18155647-18155666 Msa0592660:intron 10.0%
!! AACTTAGAAAATCATTTAAA+AGG + chr4_2:18155281-18155300 Msa0592660:CDS 15.0%
!! TAACATTAACATATGAATAG+TGG - chr4_2:18155637-18155656 None:intergenic 20.0%
!! AATTTCAAGAATACACATTA+TGG + chr4_2:18157307-18157326 Msa0592660:CDS 20.0%
!! ATTTCAAGAATACACATTAT+GGG + chr4_2:18157308-18157327 Msa0592660:CDS 20.0%
!! AAGTTTTTGCATAAATCCAA+TGG - chr4_2:18154951-18154970 None:intergenic 25.0%
!! AAGTCTTTTGCACAAATTAA+AGG - chr4_2:18155053-18155072 None:intergenic 25.0%
!! ATTCTTAATGTGGAAAGTTT+TGG - chr4_2:18155247-18155266 None:intergenic 25.0%
! TTTCCACATTAAGAATTGAT+GGG + chr4_2:18155251-18155270 Msa0592660:CDS 25.0%
!!! TCTACTTTTTCAATGCTTAA+TGG - chr4_2:18155544-18155563 None:intergenic 25.0%
!!! CAATTTGGAATTTTGGTTTT+TGG - chr4_2:18155867-18155886 None:intergenic 25.0%
! CAAAAACCAAAATTCCAAAT+TGG + chr4_2:18155865-18155884 Msa0592660:intron 25.0%
!!! TGAAGATATTCCTCTTTTTT+GGG + chr4_2:18155905-18155924 Msa0592660:intron 25.0%
! ATGAATCCAAGAGAAATATT+TGG + chr4_2:18156440-18156459 Msa0592660:CDS 25.0%
!!! TTTACCTCTTTTAGAATTAG+AGG + chr4_2:18156469-18156488 Msa0592660:CDS 25.0%
! TTGCTAAGGTTGAAAATAAA+TGG + chr4_2:18156534-18156553 Msa0592660:CDS 25.0%
! TGCTAAGGTTGAAAATAAAT+GGG + chr4_2:18156535-18156554 Msa0592660:CDS 25.0%
! GATAACATTCCTAAACATAT+TGG + chr4_2:18156647-18156666 Msa0592660:CDS 25.0%
!! AATTGTGGAACAATTTTTGA+GGG + chr4_2:18156715-18156734 Msa0592660:CDS 25.0%
!! ACGAGCTTTAATAAATCTTA+TGG + chr4_2:18157097-18157116 Msa0592660:CDS 25.0%
!! CGAGCTTTAATAAATCTTAT+GGG + chr4_2:18157098-18157117 Msa0592660:CDS 25.0%
TGGATAAGCCATTAAAGATT+TGG + chr4_2:18155101-18155120 Msa0592660:intron 30.0%
CCTATCATCCAAATCTTTAA+TGG - chr4_2:18155112-18155131 None:intergenic 30.0%
CCATTAAAGATTTGGATGAT+AGG + chr4_2:18155109-18155128 Msa0592660:intron 30.0%
GTAAGATTACCAATGGAATT+TGG - chr4_2:18155154-18155173 None:intergenic 30.0%
! AATTCCATTGGTAATCTTAC+AGG + chr4_2:18155154-18155173 Msa0592660:CDS 30.0%
! ATTCCATTGGTAATCTTACA+GGG + chr4_2:18155155-18155174 Msa0592660:CDS 30.0%
CTTTCCACATTAAGAATTGA+TGG + chr4_2:18155250-18155269 Msa0592660:CDS 30.0%
ACATTCAAGTCTTCCAAATT+CGG - chr4_2:18155415-18155434 None:intergenic 30.0%
!!! AAGATCCTTTGATGGATTTT+GGG - chr4_2:18155462-18155481 None:intergenic 30.0%
!!! AAAGATCCTTTGATGGATTT+TGG - chr4_2:18155463-18155482 None:intergenic 30.0%
TTGTGAAAGCTTAACTTCAA+AGG + chr4_2:18155573-18155592 Msa0592660:CDS 30.0%
!! GTCAAACCAATTTGGAATTT+TGG - chr4_2:18155874-18155893 None:intergenic 30.0%
!!! GTGAAGATATTCCTCTTTTT+TGG + chr4_2:18155904-18155923 Msa0592660:intron 30.0%
GGTCTGCATATATTTATTGA+TGG + chr4_2:18156059-18156078 Msa0592660:CDS 30.0%
ATACTTATGTTGGAGATGAT+TGG + chr4_2:18156192-18156211 Msa0592660:CDS 30.0%
!! ATCGTTGACTTTGAAATGTT+GGG + chr4_2:18156241-18156260 Msa0592660:CDS 30.0%
TACTCACCAAATATTTCTCT+TGG - chr4_2:18156449-18156468 None:intergenic 30.0%
! GTTTCCTCTAATTCTAAAAG+AGG - chr4_2:18156476-18156495 None:intergenic 30.0%
AACAATGCCTTTGTAAAACT+TGG - chr4_2:18156500-18156519 None:intergenic 30.0%
TACGAATCACCAATATGTTT+AGG - chr4_2:18156659-18156678 None:intergenic 30.0%
! AAACATATTGGTGATTCGTA+TGG + chr4_2:18156659-18156678 Msa0592660:CDS 30.0%
! GAATTGTGGAACAATTTTTG+AGG + chr4_2:18156714-18156733 Msa0592660:CDS 30.0%
CAAAAGAAGTCTCAAATGAT+AGG + chr4_2:18156930-18156949 Msa0592660:CDS 30.0%
AGTTTCTGACAAATATTTGC+TGG + chr4_2:18157156-18157175 Msa0592660:CDS 30.0%
! CTGGAAACTTTATTTCTTAG+AGG + chr4_2:18157175-18157194 Msa0592660:CDS 30.0%
TCTTAGAGGACAAGAAAATT+TGG + chr4_2:18157189-18157208 Msa0592660:CDS 30.0%
AATTTGGAAATGGGATTGTT+AGG + chr4_2:18157235-18157254 Msa0592660:CDS 30.0%
ATTGTTAGGATTAGGAATGT+TGG + chr4_2:18157249-18157268 Msa0592660:CDS 30.0%
TTGTTAGGATTAGGAATGTT+GGG + chr4_2:18157250-18157269 Msa0592660:CDS 30.0%
CTTACTCTTACTGTGAATAA+TGG - chr4_2:18157563-18157582 None:intergenic 30.0%
! CACATCAGGTATTTGAGTTA+TGG - chr4_2:18154861-18154880 None:intergenic 35.0%
CATTGAACCTTACCACTTTA+TGG - chr4_2:18154925-18154944 None:intergenic 35.0%
GTAAGGTTCAATGAATCCAT+TGG + chr4_2:18154932-18154951 Msa0592660:CDS 35.0%
! CCATCTTTTGCATTACTTCA+GGG - chr4_2:18155084-18155103 None:intergenic 35.0%
! TCCATCTTTTGCATTACTTC+AGG - chr4_2:18155085-18155104 None:intergenic 35.0%
AAGAGGTTTCCAAATTCCAT+TGG + chr4_2:18155142-18155161 Msa0592660:CDS 35.0%
ACACCCATCAATTCTTAATG+TGG - chr4_2:18155257-18155276 None:intergenic 35.0%
AAGGTTCAAAGAGAGCAATT+CGG + chr4_2:18155300-18155319 Msa0592660:CDS 35.0%
TCTTGAAAGTTTCCCGAATT+TGG + chr4_2:18155399-18155418 Msa0592660:CDS 35.0%
CATTCAAGTCTTCCAAATTC+GGG - chr4_2:18155414-18155433 None:intergenic 35.0%
CAAATGCAAAGATCCTTTGA+TGG - chr4_2:18155470-18155489 None:intergenic 35.0%
TCAATGCTTAATGGAAGTTC+TGG - chr4_2:18155535-18155554 None:intergenic 35.0%
TGGAGAAAATAGTGAAACCT+TGG - chr4_2:18155617-18155636 None:intergenic 35.0%
!! TTGCTTTAGCATTGGTGTTT+GGG + chr4_2:18155949-18155968 Msa0592660:CDS 35.0%
TCAGATATGTCACACATTGT+TGG + chr4_2:18156038-18156057 Msa0592660:CDS 35.0%
AATTCTTGCGACATACTTCT+TGG - chr4_2:18156085-18156104 None:intergenic 35.0%
AATTACCATTACTGCAGTGT+TGG + chr4_2:18156101-18156120 Msa0592660:CDS 35.0%
ATTACCATTACTGCAGTGTT+GGG + chr4_2:18156102-18156121 Msa0592660:CDS 35.0%
ATGTCTTAATGTGTGACCTT+AGG + chr4_2:18156132-18156151 Msa0592660:CDS 35.0%
TCATCTCCAACATAAGTATC+TGG - chr4_2:18156191-18156210 None:intergenic 35.0%
TTATGTTGGAGATGATTGGA+AGG + chr4_2:18156196-18156215 Msa0592660:CDS 35.0%
!! CATCGTTGACTTTGAAATGT+TGG + chr4_2:18156240-18156259 Msa0592660:CDS 35.0%
!! TCGTTGACTTTGAAATGTTG+GGG + chr4_2:18156242-18156261 Msa0592660:CDS 35.0%
CTTCCCAATATTAATTCCCA+TGG + chr4_2:18156320-18156339 Msa0592660:CDS 35.0%
CCACCATGGGAATTAATATT+GGG - chr4_2:18156326-18156345 None:intergenic 35.0%
ACCACCATGGGAATTAATAT+TGG - chr4_2:18156327-18156346 None:intergenic 35.0%
! GGAAACTCCAAGTTTTACAA+AGG + chr4_2:18156490-18156509 Msa0592660:CDS 35.0%
!!! GTTTTACAAAGGCATTGTTG+AGG + chr4_2:18156501-18156520 Msa0592660:CDS 35.0%
GTGGAATTTATGAGGGAAAA+AGG + chr4_2:18156743-18156762 Msa0592660:CDS 35.0%
! TTTAACACCGCTTTGAACTT+TGG - chr4_2:18156875-18156894 None:intergenic 35.0%
! TGAGACTTCTTTTGTGTTCA+AGG - chr4_2:18156924-18156943 None:intergenic 35.0%
ATCATAGTACTCTTCCTCAA+AGG - chr4_2:18157035-18157054 None:intergenic 35.0%
!! AGAGTACTATGATCCTGTTT+TGG + chr4_2:18157042-18157061 Msa0592660:CDS 35.0%
ATCATCAACTCTTCCAAAAC+AGG - chr4_2:18157058-18157077 None:intergenic 35.0%
!! AGAGTTGATGATCATGATAG+AGG + chr4_2:18157066-18157085 Msa0592660:CDS 35.0%
! CAATACTGGATTGGGAAATT+TGG + chr4_2:18157219-18157238 Msa0592660:CDS 35.0%
! TGGATTGGGAAATTTGGAAA+TGG + chr4_2:18157225-18157244 Msa0592660:CDS 35.0%
GGATTGGGAAATTTGGAAAT+GGG + chr4_2:18157226-18157245 Msa0592660:CDS 35.0%
GAAATGGGATTGTTAGGATT+AGG + chr4_2:18157241-18157260 Msa0592660:CDS 35.0%
ATTTCAGGATTTGGAAGGAT+GGG + chr4_2:18157280-18157299 Msa0592660:CDS 35.0%
ATACACATTATGGGAAGTTG+AGG + chr4_2:18157317-18157336 Msa0592660:CDS 35.0%
TACACATTATGGGAAGTTGA+GGG + chr4_2:18157318-18157337 Msa0592660:CDS 35.0%
TTACTGTGAATAATGGTCTG+AGG - chr4_2:18157556-18157575 None:intergenic 35.0%
TATTCACAGTAAGAGTAAGC+AGG + chr4_2:18157564-18157583 Msa0592660:CDS 35.0%
ATTCACAGTAAGAGTAAGCA+GGG + chr4_2:18157565-18157584 Msa0592660:CDS 35.0%
ACTCAAATACCTGATGTGTC+TGG + chr4_2:18154863-18154882 Msa0592660:CDS 40.0%
TTTATAGCTCCAGACACATC+AGG - chr4_2:18154875-18154894 None:intergenic 40.0%
ACTTGACAGATGCCATAAAG+TGG + chr4_2:18154910-18154929 Msa0592660:CDS 40.0%
ACAGATGCCATAAAGTGGTA+AGG + chr4_2:18154915-18154934 Msa0592660:CDS 40.0%
! TGGGACAAAACTTTTGAGCA+TGG - chr4_2:18154999-18155018 None:intergenic 40.0%
AAGGAGAGAACTTCAAGAGA+TGG - chr4_2:18155034-18155053 None:intergenic 40.0%
CCCTGAAGTAATGCAAAAGA+TGG + chr4_2:18155081-18155100 Msa0592660:intron 40.0%
! TGATAGGTACTGCTGTTAAG+AGG + chr4_2:18155125-18155144 Msa0592660:intron 40.0%
AAGCCCTGTAAGATTACCAA+TGG - chr4_2:18155161-18155180 None:intergenic 40.0%
TGGAAGTAAGAAGAAGCTAC+TGG - chr4_2:18155227-18155246 None:intergenic 40.0%
TCTCTCCCAAAATCCATCAA+AGG + chr4_2:18155454-18155473 Msa0592660:CDS 40.0%
GAGTTATTGCAGGAGTCTTA+TGG + chr4_2:18155504-18155523 Msa0592660:CDS 40.0%
!! CAAAGGCATCAAGCATTCTA+TGG + chr4_2:18155590-18155609 Msa0592660:CDS 40.0%
ATCAAGCATTCTATGGTCCA+AGG + chr4_2:18155597-18155616 Msa0592660:CDS 40.0%
TTTCTACCTGTTGATGTCAC+AGG + chr4_2:18155706-18155725 Msa0592660:intron 40.0%
CCAACACAGTCAAACCAATT+TGG - chr4_2:18155882-18155901 None:intergenic 40.0%
!! CCAAATTGGTTTGACTGTGT+TGG + chr4_2:18155879-18155898 Msa0592660:intron 40.0%
CCAATGCTAAAGCAACCATA+GGG - chr4_2:18155944-18155963 None:intergenic 40.0%
ACCAATGCTAAAGCAACCAT+AGG - chr4_2:18155945-18155964 None:intergenic 40.0%
!! GTTGCTTTAGCATTGGTGTT+TGG + chr4_2:18155948-18155967 Msa0592660:CDS 40.0%
!! TGCTTTAGCATTGGTGTTTG+GGG + chr4_2:18155950-18155969 Msa0592660:CDS 40.0%
CGGTATCTCGTTATTCTGTT+GGG - chr4_2:18155979-18155998 None:intergenic 40.0%
TCGGTATCTCGTTATTCTGT+TGG - chr4_2:18155980-18155999 None:intergenic 40.0%
TCGGAGATGAATGTGTCTTT+CGG - chr4_2:18155999-18156018 None:intergenic 40.0%
ACATATCTGACATCAGTCCA+GGG - chr4_2:18156028-18156047 None:intergenic 40.0%
TTACCATTACTGCAGTGTTG+GGG + chr4_2:18156103-18156122 Msa0592660:CDS 40.0%
TGTTGCTGAAGAAAGTCCTA+AGG - chr4_2:18156151-18156170 None:intergenic 40.0%
TCTTCAGCAACAATGAAGAG+TGG + chr4_2:18156159-18156178 Msa0592660:CDS 40.0%
CCCAATATTAATTCCCATGG+TGG + chr4_2:18156323-18156342 Msa0592660:CDS 40.0%
! GACATCTGAGGAGATCTTTT+TGG - chr4_2:18156389-18156408 None:intergenic 40.0%
ATTCTTTGGACAGACATCTG+AGG - chr4_2:18156401-18156420 None:intergenic 40.0%
! TTGCTTTAAACTTCCCCCAA+AGG - chr4_2:18156576-18156595 None:intergenic 40.0%
! AAGCAAGAGCATGAAGAATC+TGG + chr4_2:18156590-18156609 Msa0592660:CDS 40.0%
! AAGAGCATGAAGAATCTGGA+TGG + chr4_2:18156594-18156613 Msa0592660:CDS 40.0%
AGAGCATGAAGAATCTGGAT+GGG + chr4_2:18156595-18156614 Msa0592660:CDS 40.0%
ACAAAGTGCACAACGAATTG+TGG + chr4_2:18156700-18156719 Msa0592660:CDS 40.0%
!! TGGAACAATTTTTGAGGGCA+AGG + chr4_2:18156720-18156739 Msa0592660:CDS 40.0%
!! GGAACAATTTTTGAGGGCAA+GGG + chr4_2:18156721-18156740 Msa0592660:CDS 40.0%
TGAGGGAAAAAGGTAATGAG+AGG + chr4_2:18156753-18156772 Msa0592660:CDS 40.0%
GGAAAAAGGTAATGAGAGGT+TGG + chr4_2:18156757-18156776 Msa0592660:CDS 40.0%
TGACATGCTTCAAGGACAAT+AGG - chr4_2:18156794-18156813 None:intergenic 40.0%
!! GTCCTTGAAGCATGTCATTT+TGG + chr4_2:18156797-18156816 Msa0592660:CDS 40.0%
CACCAAAATGACATGCTTCA+AGG - chr4_2:18156802-18156821 None:intergenic 40.0%
CGAAGATCCAAAGTTCAAAG+CGG + chr4_2:18156865-18156884 Msa0592660:CDS 40.0%
! AACTTCAAGCTCTAGCTATG+AGG + chr4_2:18157003-18157022 Msa0592660:CDS 40.0%
CTATGAGGAAGAATCCTTTG+AGG + chr4_2:18157018-18157037 Msa0592660:CDS 40.0%
!! GTTGATGATCATGATAGAGG+AGG + chr4_2:18157069-18157088 Msa0592660:CDS 40.0%
GGACAAGAAAATTTGGCACA+AGG + chr4_2:18157196-18157215 Msa0592660:CDS 40.0%
AATTTGGCACAAGGCAATAC+TGG + chr4_2:18157205-18157224 Msa0592660:CDS 40.0%
GATTAGGAATGTTGGGATCT+GGG + chr4_2:18157257-18157276 Msa0592660:CDS 40.0%
GATTTCAGGATTTGGAAGGA+TGG + chr4_2:18157279-18157298 Msa0592660:CDS 40.0%
CTCAGAAACAGAAAGAGTCA+AGG + chr4_2:18157368-18157387 Msa0592660:CDS 40.0%
AGAAACAGAAAGAGTCAAGG+AGG + chr4_2:18157371-18157390 Msa0592660:CDS 40.0%
TCAGACACAGAAAGAGTCAA+CGG + chr4_2:18157468-18157487 Msa0592660:CDS 40.0%
ATGAAATGCAGCATGAGTTG+AGG + chr4_2:18157527-18157546 Msa0592660:CDS 40.0%
! TGAAATGCAGCATGAGTTGA+GGG + chr4_2:18157528-18157547 Msa0592660:CDS 40.0%
GAGATGGCAAGTACATGCTT+GGG - chr4_2:18155018-18155037 None:intergenic 45.0%
AGAGATGGCAAGTACATGCT+TGG - chr4_2:18155019-18155038 None:intergenic 45.0%
GAGAGCAATTCGGTAGCAAA+TGG + chr4_2:18155310-18155329 Msa0592660:CDS 45.0%
AGCAAATGGCAGCTCAAGTT+TGG + chr4_2:18155324-18155343 Msa0592660:CDS 45.0%
GTCTTGACGTGAGTTATTGC+AGG + chr4_2:18155494-18155513 Msa0592660:CDS 45.0%
AAGCAACCATAGGGAACTTC+CGG - chr4_2:18155935-18155954 None:intergenic 45.0%
! CCCTATGGTTGCTTTAGCAT+TGG + chr4_2:18155941-18155960 Msa0592660:CDS 45.0%
GACATATCTGACATCAGTCC+AGG - chr4_2:18156029-18156048 None:intergenic 45.0%
TTTCCCCAACACTGCAGTAA+TGG - chr4_2:18156109-18156128 None:intergenic 45.0%
AGCAACAATGAAGAGTGGCA+GGG + chr4_2:18156164-18156183 Msa0592660:CDS 45.0%
CAGGGTCCAGATACTTATGT+TGG + chr4_2:18156182-18156201 Msa0592660:CDS 45.0%
AGATGATTGGAAGGCTGTTC+AGG + chr4_2:18156205-18156224 Msa0592660:CDS 45.0%
! CTATGTGCCATTGCCAATGT+CGG + chr4_2:18156349-18156368 Msa0592660:CDS 45.0%
CCAAAGAATGAGACATGCGT+CGG + chr4_2:18156412-18156431 Msa0592660:CDS 45.0%
AAGCTTCAGCCATTGCCTTT+GGG + chr4_2:18156558-18156577 Msa0592660:CDS 45.0%
TAAACTTCCCCCAAAGGCAA+TGG - chr4_2:18156570-18156589 None:intergenic 45.0%
!! GCGAGTTGTAGAGTTGATGA+AGG + chr4_2:18156625-18156644 Msa0592660:CDS 45.0%
!! ACAATTTTTGAGGGCAAGGG+TGG + chr4_2:18156724-18156743 Msa0592660:CDS 45.0%
GGCAAGGGTGGAATTTATGA+GGG + chr4_2:18156736-18156755 Msa0592660:CDS 45.0%
AAGCTAAGAGTCGTCGCTAT+TGG + chr4_2:18156822-18156841 Msa0592660:CDS 45.0%
AGCTAAGAGTCGTCGCTATT+GGG + chr4_2:18156823-18156842 Msa0592660:CDS 45.0%
ACAAGACAAGCCAACTTGCA+TGG + chr4_2:18156894-18156913 Msa0592660:CDS 45.0%
TCAAGGCTTTCCATGCAAGT+TGG - chr4_2:18156907-18156926 None:intergenic 45.0%
GTTGAAGCTTCCTCTGTAGA+TGG - chr4_2:18156986-18157005 None:intergenic 45.0%
GCACAAGGCAATACTGGATT+GGG + chr4_2:18157211-18157230 Msa0592660:CDS 45.0%
GGATTAGGAATGTTGGGATC+TGG + chr4_2:18157256-18157275 Msa0592660:CDS 45.0%
! ATGTTGGGATCTGGGATTTC+AGG + chr4_2:18157265-18157284 Msa0592660:CDS 45.0%
! GGATCTGGGATTTCAGGATT+TGG + chr4_2:18157271-18157290 Msa0592660:CDS 45.0%
CTGGGATTTCAGGATTTGGA+AGG + chr4_2:18157275-18157294 Msa0592660:CDS 45.0%
ACATTATGGGAAGTTGAGGG+CGG + chr4_2:18157321-18157340 Msa0592660:CDS 45.0%
AACAGAAAGAGTCAAGGAGG+AGG + chr4_2:18157374-18157393 Msa0592660:CDS 45.0%
ATCGAATGCAGCATGAGTTG+AGG + chr4_2:18157428-18157447 Msa0592660:CDS 45.0%
! TCGAATGCAGCATGAGTTGA+GGG + chr4_2:18157429-18157448 Msa0592660:CDS 45.0%
ACAGTAAGAGTAAGCAGGGT+TGG + chr4_2:18157569-18157588 Msa0592660:CDS 45.0%
! GTTGGATCACGGCTTTTATG+AGG + chr4_2:18157587-18157606 Msa0592660:CDS 45.0%
CGCAAACCTGTGACATCAAC+AGG - chr4_2:18155715-18155734 None:intergenic 50.0%
GTCACAGGTTTGCGATGAGA+AGG + chr4_2:18155721-18155740 Msa0592660:intron 50.0%
!!! TTCCTCTTTTTTGGGCTCGC+CGG + chr4_2:18155913-18155932 Msa0592660:intron 50.0%
TTCATCTCCGACTTCTTCCC+TGG + chr4_2:18156008-18156027 Msa0592660:CDS 50.0%
CATCAGTCCAGGGAAGAAGT+CGG - chr4_2:18156018-18156037 None:intergenic 50.0%
CAGCAACAATGAAGAGTGGC+AGG + chr4_2:18156163-18156182 Msa0592660:CDS 50.0%
TGGCACATAGTCACCACCAT+GGG - chr4_2:18156339-18156358 None:intergenic 50.0%
ATGGCACATAGTCACCACCA+TGG - chr4_2:18156340-18156359 None:intergenic 50.0%
ACCAAACCTGATGCCGACAT+TGG - chr4_2:18156365-18156384 None:intergenic 50.0%
CCGACGCATGTCTCATTCTT+TGG - chr4_2:18156415-18156434 None:intergenic 50.0%
!! CAAAGGCATTGTTGAGGTCG+TGG + chr4_2:18156507-18156526 Msa0592660:CDS 50.0%
GAAGCTTCAGCCATTGCCTT+TGG + chr4_2:18156557-18156576 Msa0592660:CDS 50.0%
AGCTTCAGCCATTGCCTTTG+GGG + chr4_2:18156559-18156578 Msa0592660:CDS 50.0%
GGGCAAGGGTGGAATTTATG+AGG + chr4_2:18156735-18156754 Msa0592660:CDS 50.0%
GCTAAGAGTCGTCGCTATTG+GGG + chr4_2:18156824-18156843 Msa0592660:CDS 50.0%
ATGCCAGAGACCATCTACAG+AGG + chr4_2:18156973-18156992 Msa0592660:CDS 50.0%
CTTCCTCTGTAGATGGTCTC+TGG - chr4_2:18156979-18156998 None:intergenic 50.0%
GGCACAAGGCAATACTGGAT+TGG + chr4_2:18157210-18157229 Msa0592660:CDS 50.0%
! AATGCAGCATGAGTTGAGGG+TGG + chr4_2:18157432-18157451 Msa0592660:CDS 50.0%
GAGTAAGCAGGGTTGGATCA+CGG + chr4_2:18157576-18157595 Msa0592660:CDS 50.0%
! GGATCACGGCTTTTATGAGG+TGG + chr4_2:18157590-18157609 Msa0592660:CDS 50.0%
TTCCGGCGAGCCCAAAAAAG+AGG - chr4_2:18155918-18155937 None:intergenic 55.0%
CCTGATGCCGACATTGGCAA+TGG - chr4_2:18156359-18156378 None:intergenic 55.0%
CCATTGCCAATGTCGGCATC+AGG + chr4_2:18156356-18156375 Msa0592660:CDS 55.0%
GCCAATGTCGGCATCAGGTT+TGG + chr4_2:18156361-18156380 Msa0592660:CDS 55.0%
! AGGCATTGTTGAGGTCGTGG+AGG + chr4_2:18156510-18156529 Msa0592660:CDS 55.0%
GAGGTCGTGGAGGATTGCTA+AGG + chr4_2:18156520-18156539 Msa0592660:CDS 55.0%
GCTTCAGCCATTGCCTTTGG+GGG + chr4_2:18156560-18156579 Msa0592660:CDS 55.0%
GGCTCGCCGGAAGTTCCCTA+TGG + chr4_2:18155926-18155945 Msa0592660:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 18154815 18157639 18154815 ID=Msa0592660;Name=Msa0592660
chr4_2 mRNA 18154815 18157639 18154815 ID=Msa0592660-mRNA-1;Parent=Msa0592660;Name=Msa0592660-mRNA-1;_AED=0.16;_eAED=0.19;_QI=0|0|0|1|0|0|4|0|787
chr4_2 exon 18154815 18155007 18154815 ID=Msa0592660-mRNA-1:exon:5230;Parent=Msa0592660-mRNA-1
chr4_2 exon 18155131 18155361 18155131 ID=Msa0592660-mRNA-1:exon:5231;Parent=Msa0592660-mRNA-1
chr4_2 exon 18155380 18155618 18155380 ID=Msa0592660-mRNA-1:exon:5232;Parent=Msa0592660-mRNA-1
chr4_2 exon 18155939 18157639 18155939 ID=Msa0592660-mRNA-1:exon:5233;Parent=Msa0592660-mRNA-1
chr4_2 CDS 18154815 18155007 18154815 ID=Msa0592660-mRNA-1:cds;Parent=Msa0592660-mRNA-1
chr4_2 CDS 18155131 18155361 18155131 ID=Msa0592660-mRNA-1:cds;Parent=Msa0592660-mRNA-1
chr4_2 CDS 18155380 18155618 18155380 ID=Msa0592660-mRNA-1:cds;Parent=Msa0592660-mRNA-1
chr4_2 CDS 18155939 18157639 18155939 ID=Msa0592660-mRNA-1:cds;Parent=Msa0592660-mRNA-1
Gene Sequence

>Msa0592660

AAATTTGAGGATTTGACATTCATGAATCTCTCTCAGTGTCAATCCATAACTCAAATACCTGATGTGTCTGGAGCTATAAATCTAAGAGTATTGACACTTGACAGATGCCATAAAGTGGTAAGGTTCAATGAATCCATTGGATTTATGCAAAAACTTGTTTGTTTAAGTGCTTCAGAGTGTACCATGCTCAAAAGTACTGCTGTTAAGAGGTTTCCAAATTCCATTGGTAATCTTACAGGGCTTGAATACATAGACATGTCATTTTGCAGAAGACTTAGCGATCTATCCAGTAGCTTCTTCTTACTTCCAAAACTTTCCACATTAAGAATTGATGGGTGTTCTCAACTTAGAAAATCATTTAAAAGGTTCAAAGAGAGCAATTCGGTAGCAAATGGCAGCTCAAGTTTGGTGACACTGTACTTAAATGAAGATCTTAACACGATTCTTGAAAGTTTCCCGAATTTGGAAGACTTGAATGTGTCACACAATAAATTTGCATCTCTCCCAAAATCCATCAAAGGATCTTTGCATTTGAAAAGTCTTGACGTGAGTTATTGCAGGAGTCTTATGGAAATTCCAGAACTTCCATTAAGCATTGAAAAAGTAGATGCAAGATATTGTGAAAGCTTAACTTCAAAGGCATCAAGCATTCTATGGTCCAAGTTCCCTATGGTTGCTTTAGCATTGGTGTTTGGGGAATCCCAACAGAATAACGAGATACCGAAAGACACATTCATCTCCGACTTCTTCCCTGGACTGATGTCAGATATGTCACACATTGTTGGTCTGCATATATTTATTGATGGCCAAGAAGTATGTCGCAAGAATTACCATTACTGCAGTGTTGGGGAAAATCATGTCTTAATGTGTGACCTTAGGACTTTCTTCAGCAACAATGAAGAGTGGCAGGGTCCAGATACTTATGTTGGAGATGATTGGAAGGCTGTTCAGGTTCAATGCGAATCATCGTTGACTTTGAAATGTTGGGGAGTTCATGTTTTCAAACAGAAAACAGACAATGATGATATTGATATCTCATTTAAGCTTCCCAATATTAATTCCCATGGTGGTGACTATGTGCCATTGCCAATGTCGGCATCAGGTTTGGTTCCAAAAAGATCTCCTCAGATGTCTGTCCAAAGAATGAGACATGCGTCGGAAAATATGAATCCAAGAGAAATATTTGGTGAGTATTTACCTCTTTTAGAATTAGAGGAAACTCCAAGTTTTACAAAGGCATTGTTGAGGTCGTGGAGGATTGCTAAGGTTGAAAATAAATGGGAAGCTTCAGCCATTGCCTTTGGGGGAAGTTTAAAGCAAGAGCATGAAGAATCTGGATGGGATGTTGTGCGAGTTGTAGAGTTGATGAAGGATAACATTCCTAAACATATTGGTGATTCGTATGGTTTCGATAACAATGACATACAAAGTGCACAACGAATTGTGGAACAATTTTTGAGGGCAAGGGTGGAATTTATGAGGGAAAAAGGTAATGAGAGGTTGGATATTGATATGCCTATTGTCCTTGAAGCATGTCATTTTGGTGAAGCTAAGAGTCGTCGCTATTGGGGAAAGTTGCAAATAAAACACGAAGATCCAAAGTTCAAAGCGGTGTTAAACAAGACAAGCCAACTTGCATGGAAAGCCTTGAACACAAAAGAAGTCTCAAATGATAGGATGAACATTGTTCTATTAAAATGCCAGAGACCATCTACAGAGGAAGCTTCAACTTCAAGCTCTAGCTATGAGGAAGAATCCTTTGAGGAAGAGTACTATGATCCTGTTTTGGAAGAGTTGATGATCATGATAGAGGAGGATGCTACGAGCTTTAATAAATCTTATGGGATTTTGAAAGCATCTATTGTTCTAACTCATGAACTAGTTTCTGACAAATATTTGCTGGAAACTTTATTTCTTAGAGGACAAGAAAATTTGGCACAAGGCAATACTGGATTGGGAAATTTGGAAATGGGATTGTTAGGATTAGGAATGTTGGGATCTGGGATTTCAGGATTTGGAAGGATGGGATCAAATTTCAAGAATACACATTATGGGAAGTTGAGGGCGGAAGACGATGATACTAAGAGCACTGCTCAGAAACAGAAAGAGTCAAGGAGGAGGCGTGATGTTCAGCAAACTGTATATAGTCAGAATCGAATGCAGCATGAGTTGAGGGTGGAAGATGATAATACTCAGACACAGAAAGAGTCAACGGTGATTCACGTTGTTCAGCAAACTGTACATAATCAGAATGAAATGCAGCATGAGTTGAGGGATCCTCAGACCATTATTCACAGTAAGAGTAAGCAGGGTTGGATCACGGCTTTTATGAGGTGGATTCTGTGTTGTTGTACCCCCAAATGA
Protein sequence

>Msa0592660

KFEDLTFMNLSQCQSITQIPDVSGAINLRVLTLDRCHKVVRFNESIGFMQKLVCLSASECTMLKSTAVKRFPNSIGNLTGLEYIDMSFCRRLSDLSSSFFLLPKLSTLRIDGCSQLRKSFKRFKESNSVANGSSSLVTLYLNEDLNTILESFPNLEDLNVSHNKFASLPKSIKGSLHLKSLDVSYCRSLMEIPELPLSIEKVDARYCESLTSKASSILWSKFPMVALALVFGESQQNNEIPKDTFISDFFPGLMSDMSHIVGLHIFIDGQEVCRKNYHYCSVGENHVLMCDLRTFFSNNEEWQGPDTYVGDDWKAVQVQCESSLTLKCWGVHVFKQKTDNDDIDISFKLPNINSHGGDYVPLPMSASGLVPKRSPQMSVQRMRHASENMNPREIFGEYLPLLELEETPSFTKALLRSWRIAKVENKWEASAIAFGGSLKQEHEESGWDVVRVVELMKDNIPKHIGDSYGFDNNDIQSAQRIVEQFLRARVEFMREKGNERLDIDMPIVLEACHFGEAKSRRYWGKLQIKHEDPKFKAVLNKTSQLAWKALNTKEVSNDRMNIVLLKCQRPSTEEASTSSSSYEEESFEEEYYDPVLEELMIMIEEDATSFNKSYGILKASIVLTHELVSDKYLLETLFLRGQENLAQGNTGLGNLEMGLLGLGMLGSGISGFGRMGSNFKNTHYGKLRAEDDDTKSTAQKQKESRRRRDVQQTVYSQNRMQHELRVEDDNTQTQKESTVIHVVQQTVHNQNEMQHELRDPQTIIHSKSKQGWITAFMRWILCCCTPK*