AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1436090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1436090 MtrunA17_Chr3g0134881 87.448 239 23 2 1 239 1 232 2.60e-137 385
Msa1436090 MtrunA17_Chr1g0152461 40.264 303 99 10 1 239 1 285 9.50e-57 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1436090 AT1G59590.1 36.098 205 95 8 19 211 8 188 3.78e-30 112
Msa1436090 AT1G10220.1 36.898 187 102 6 19 200 4 179 1.23e-24 97.4
Msa1436090 AT1G10220.2 33.862 189 116 4 19 200 4 190 1.91e-23 94.7

Find 29 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAACTATCTTTCAAAAT+AGG 0.247880 tig0027852:-26373 None:intergenic
GGAAATTGCCACAAACTAAA+TGG 0.257314 tig0027852:-25805 None:intergenic
AGAGAAATTGATGAAGAAAA+AGG 0.259298 tig0027852:+26399 Msa1436090:CDS
TTTGATCAAGTTCAGATAGA+AGG 0.288309 tig0027852:-25943 None:intergenic
CCAAAAGAAAAGACAACAAT+AGG 0.309829 tig0027852:+25876 Msa1436090:CDS
TCAAACAAGATTGAAAGCTT+TGG 0.359242 tig0027852:+26220 Msa1436090:CDS
ATGGTATATCATCTTCTAAT+TGG 0.359629 tig0027852:-25832 None:intergenic
ATGCTTAACCATTTAGTTTG+TGG 0.373237 tig0027852:+25797 Msa1436090:CDS
TCTAAGCCTCTTGAAGCATA+AGG 0.392424 tig0027852:-25908 None:intergenic
TCAACATGTTTCTGATCCAT+TGG 0.398061 tig0027852:-26139 None:intergenic
TTGTTGTTGTTGACTGTGTT+TGG 0.422356 tig0027852:+26304 Msa1436090:CDS
GCTTCTAATGATAAGAGATC+TGG 0.433001 tig0027852:+26466 Msa1436090:CDS
TATCATCTTCTAATTGGTGT+TGG 0.486872 tig0027852:-25826 None:intergenic
CAAGAGTGAAGAACAAGTTA+AGG 0.497546 tig0027852:+26090 Msa1436090:CDS
TATCTGAACTTGATCAAAGA+AGG 0.501411 tig0027852:+25948 Msa1436090:CDS
AAAATCAATATCACAACAGC+AGG 0.519128 tig0027852:-26283 None:intergenic
ATGATGAGATATTTCCAACT+TGG 0.523515 tig0027852:-26176 None:intergenic
CAAAAGAAAAGACAACAATA+GGG 0.523809 tig0027852:+25877 Msa1436090:CDS
AAAAGGATTATGTTACTGAA+GGG 0.526034 tig0027852:+26416 Msa1436090:CDS
AGAAACAAAACAAGCCAAGT+TGG 0.528975 tig0027852:+26162 Msa1436090:CDS
TCTCTTTGAATATGTTGAAG+AGG 0.529203 tig0027852:+26245 Msa1436090:CDS
TTGATCAAGTTCAGATAGAA+GGG 0.545038 tig0027852:-25942 None:intergenic
AATAATCCTTATGCTTCAAG+AGG 0.562509 tig0027852:+25902 Msa1436090:CDS
GAAGCTTTAGAATCACCTCT+AGG 0.571693 tig0027852:-26448 None:intergenic
CGAACAAAAGATATGTCATG+TGG 0.588570 tig0027852:-25995 None:intergenic
ACAGCAGGCACATAGAAAGA+AGG 0.600782 tig0027852:-26268 None:intergenic
CGCTTTGTACGTGTATTGTG+TGG 0.626912 tig0027852:+26341 Msa1436090:CDS
GAACAAAAGATATGTCATGT+GGG 0.637973 tig0027852:-25994 None:intergenic
GAAGGGTTACTTTCTCCTAG+AGG 0.682377 tig0027852:+26433 Msa1436090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGGTATATCATCTTCTAAT+TGG - tig0027852:25835-25854 None:intergenic 25.0%
!! TTCTTTTGGATTTGGATTTT+GGG - tig0027852:25865-25884 None:intergenic 25.0%
!! TTTCTTTTGGATTTGGATTT+TGG - tig0027852:25866-25885 None:intergenic 25.0%
! CAAAAGAAAAGACAACAATA+GGG + tig0027852:25877-25896 Msa1436090:CDS 25.0%
!! TTTTTCACTTTGACAACAAT+TGG - tig0027852:26049-26068 None:intergenic 25.0%
! TTCGAACTATCTTTCAAAAT+AGG - tig0027852:26376-26395 None:intergenic 25.0%
! AGAGAAATTGATGAAGAAAA+AGG + tig0027852:26399-26418 Msa1436090:CDS 25.0%
! AAAAAGGATTATGTTACTGA+AGG + tig0027852:26415-26434 Msa1436090:CDS 25.0%
! AAAAGGATTATGTTACTGAA+GGG + tig0027852:26416-26435 Msa1436090:CDS 25.0%
!! ATGCTTAACCATTTAGTTTG+TGG + tig0027852:25797-25816 Msa1436090:CDS 30.0%
TATCATCTTCTAATTGGTGT+TGG - tig0027852:25829-25848 None:intergenic 30.0%
! GTTGTCTTTTCTTTTGGATT+TGG - tig0027852:25873-25892 None:intergenic 30.0%
! CCTATTGTTGTCTTTTCTTT+TGG - tig0027852:25879-25898 None:intergenic 30.0%
CCAAAAGAAAAGACAACAAT+AGG + tig0027852:25876-25895 Msa1436090:CDS 30.0%
AATAATCCTTATGCTTCAAG+AGG + tig0027852:25902-25921 Msa1436090:CDS 30.0%
TTGATCAAGTTCAGATAGAA+GGG - tig0027852:25945-25964 None:intergenic 30.0%
TTTGATCAAGTTCAGATAGA+AGG - tig0027852:25946-25965 None:intergenic 30.0%
TATCTGAACTTGATCAAAGA+AGG + tig0027852:25948-25967 Msa1436090:CDS 30.0%
GAACAAAAGATATGTCATGT+GGG - tig0027852:25997-26016 None:intergenic 30.0%
! TACATCTTTTTCTGAACCAA+TGG + tig0027852:26123-26142 Msa1436090:CDS 30.0%
ATGATGAGATATTTCCAACT+TGG - tig0027852:26179-26198 None:intergenic 30.0%
TCAAACAAGATTGAAAGCTT+TGG + tig0027852:26220-26239 Msa1436090:CDS 30.0%
TCTCTTTGAATATGTTGAAG+AGG + tig0027852:26245-26264 Msa1436090:CDS 30.0%
AAAATCAATATCACAACAGC+AGG - tig0027852:26286-26305 None:intergenic 30.0%
GGAAATTGCCACAAACTAAA+TGG - tig0027852:25808-25827 None:intergenic 35.0%
CGAACAAAAGATATGTCATG+TGG - tig0027852:25998-26017 None:intergenic 35.0%
CAAGAGTGAAGAACAAGTTA+AGG + tig0027852:26090-26109 Msa1436090:CDS 35.0%
! TCAACATGTTTCTGATCCAT+TGG - tig0027852:26142-26161 None:intergenic 35.0%
AGAAACAAAACAAGCCAAGT+TGG + tig0027852:26162-26181 Msa1436090:CDS 35.0%
! TTGTTGTTGTTGACTGTGTT+TGG + tig0027852:26304-26323 Msa1436090:CDS 35.0%
GCTTCTAATGATAAGAGATC+TGG + tig0027852:26466-26485 Msa1436090:CDS 35.0%
AACATAGCCAACAAAAAAGC+TGG + tig0027852:26491-26510 Msa1436090:CDS 35.0%
! TTGGATTTTGGGCTTGAACA+TGG - tig0027852:25854-25873 None:intergenic 40.0%
TCTAAGCCTCTTGAAGCATA+AGG - tig0027852:25911-25930 None:intergenic 40.0%
!! GAAGCTTTAGAATCACCTCT+AGG - tig0027852:26451-26470 None:intergenic 40.0%
ACAGCAGGCACATAGAAAGA+AGG - tig0027852:26271-26290 None:intergenic 45.0%
CGCTTTGTACGTGTATTGTG+TGG + tig0027852:26341-26360 Msa1436090:CDS 45.0%
! GAAGGGTTACTTTCTCCTAG+AGG + tig0027852:26433-26452 Msa1436090:CDS 45.0%
Chromosome Type Strat End Strand Name
tig0027852 gene 25797 26516 25797 ID=Msa1436090;Name=Msa1436090
tig0027852 mRNA 25797 26516 25797 ID=Msa1436090-mRNA-1;Parent=Msa1436090;Name=Msa1436090-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|239
tig0027852 exon 25797 26516 25797 ID=Msa1436090-mRNA-1:exon:10087;Parent=Msa1436090-mRNA-1
tig0027852 CDS 25797 26516 25797 ID=Msa1436090-mRNA-1:cds;Parent=Msa1436090-mRNA-1
Gene Sequence

>Msa1436090

ATGCTTAACCATTTAGTTTGTGGCAATTTCCAACACCAATTAGAAGATGATATACCATGTTCAAGCCCAAAATCCAAATCCAAAAGAAAAGACAACAATAGGGACAATAATCCTTATGCTTCAAGAGGCTTAGATAAATTTTCTACCCTTCTATCTGAACTTGATCAAAGAAGGAAGAAAGTTTACTCACAAATGAACCCACATGACATATCTTTTGTTCGTTTTACATATTCAAGTGATGATTTTGTTCCAATTGTTGTCAAAGTGAAAAATAACAATCATCAGAAGAAACACAAGAGTGAAGAACAAGTTAAGGTAAGACATGTTACATCTTTTTCTGAACCAATGGATCAGAAACATGTTGAAGAAACAAAACAAGCCAAGTTGGAAATATCTCATCATGTTGACGATGTTAAAAATGTATCAAACAAGATTGAAAGCTTTGGATTCTCTTTGAATATGTTGAAGAGGCCTTCTTTCTATGTGCCTGCTGTTGTGATATTGATTTTGTTGTTGTTGACTGTGTTTGGAAGATCATTTGCAACGCTTTGTACGTGTATTGTGTGGTATATAGTTCCTATTTTGAAAGATAGTTCGAAAGAAGAGAAATTGATGAAGAAAAAGGATTATGTTACTGAAGGGTTACTTTCTCCTAGAGGTGATTCTAAAGCTTCTAATGATAAGAGATCTGGAAAACATAGCCAACAAAAAAGCTGGTGA

Protein sequence

>Msa1436090

MLNHLVCGNFQHQLEDDIPCSSPKSKSKRKDNNRDNNPYASRGLDKFSTLLSELDQRRKKVYSQMNPHDISFVRFTYSSDDFVPIVVKVKNNNHQKKHKSEEQVKVRHVTSFSEPMDQKHVEETKQAKLEISHHVDDVKNVSNKIESFGFSLNMLKRPSFYVPAVVILILLLLTVFGRSFATLCTCIVWYIVPILKDSSKEEKLMKKKDYVTEGLLSPRGDSKASNDKRSGKHSQQKSW*