AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1413800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413800 MtrunA17_Chr3g0134881 87.448 239 23 2 1 239 1 232 2.60e-137 385
Msa1413800 MtrunA17_Chr1g0152461 40.264 303 99 10 1 239 1 285 9.50e-57 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413800 AT1G59590.1 36.098 205 95 8 19 211 8 188 3.78e-30 112
Msa1413800 AT1G10220.1 36.898 187 102 6 19 200 4 179 1.23e-24 97.4
Msa1413800 AT1G10220.2 33.862 189 116 4 19 200 4 190 1.91e-23 94.7

Find 29 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAACTATCTTTCAAAAT+AGG 0.247880 tig0024068:-25840 None:intergenic
GGAAATTGCCACAAACTAAA+TGG 0.257314 tig0024068:-25272 None:intergenic
AGAGAAATTGATGAAGAAAA+AGG 0.259298 tig0024068:+25866 Msa1413800:CDS
TTTGATCAAGTTCAGATAGA+AGG 0.288309 tig0024068:-25410 None:intergenic
CCAAAAGAAAAGACAACAAT+AGG 0.309829 tig0024068:+25343 Msa1413800:CDS
TCAAACAAGATTGAAAGCTT+TGG 0.359242 tig0024068:+25687 Msa1413800:CDS
ATGGTATATCATCTTCTAAT+TGG 0.359629 tig0024068:-25299 None:intergenic
ATGCTTAACCATTTAGTTTG+TGG 0.373237 tig0024068:+25264 Msa1413800:CDS
TCTAAGCCTCTTGAAGCATA+AGG 0.392424 tig0024068:-25375 None:intergenic
TCAACATGTTTCTGATCCAT+TGG 0.398061 tig0024068:-25606 None:intergenic
TTGTTGTTGTTGACTGTGTT+TGG 0.422356 tig0024068:+25771 Msa1413800:CDS
GCTTCTAATGATAAGAGATC+TGG 0.433001 tig0024068:+25933 Msa1413800:CDS
TATCATCTTCTAATTGGTGT+TGG 0.486872 tig0024068:-25293 None:intergenic
CAAGAGTGAAGAACAAGTTA+AGG 0.497546 tig0024068:+25557 Msa1413800:CDS
TATCTGAACTTGATCAAAGA+AGG 0.501411 tig0024068:+25415 Msa1413800:CDS
AAAATCAATATCACAACAGC+AGG 0.519128 tig0024068:-25750 None:intergenic
ATGATGAGATATTTCCAACT+TGG 0.523515 tig0024068:-25643 None:intergenic
CAAAAGAAAAGACAACAATA+GGG 0.523809 tig0024068:+25344 Msa1413800:CDS
AAAAGGATTATGTTACTGAA+GGG 0.526034 tig0024068:+25883 Msa1413800:CDS
AGAAACAAAACAAGCCAAGT+TGG 0.528975 tig0024068:+25629 Msa1413800:CDS
TCTCTTTGAATATGTTGAAG+AGG 0.529203 tig0024068:+25712 Msa1413800:CDS
TTGATCAAGTTCAGATAGAA+GGG 0.545038 tig0024068:-25409 None:intergenic
AATAATCCTTATGCTTCAAG+AGG 0.562509 tig0024068:+25369 Msa1413800:CDS
GAAGCTTTAGAATCACCTCT+AGG 0.571693 tig0024068:-25915 None:intergenic
CGAACAAAAGATATGTCATG+TGG 0.588570 tig0024068:-25462 None:intergenic
ACAGCAGGCACATAGAAAGA+AGG 0.600782 tig0024068:-25735 None:intergenic
CGCTTTGTACGTGTATTGTG+TGG 0.626912 tig0024068:+25808 Msa1413800:CDS
GAACAAAAGATATGTCATGT+GGG 0.637973 tig0024068:-25461 None:intergenic
GAAGGGTTACTTTCTCCTAG+AGG 0.682377 tig0024068:+25900 Msa1413800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGGTATATCATCTTCTAAT+TGG - tig0024068:25302-25321 None:intergenic 25.0%
!! TTCTTTTGGATTTGGATTTT+GGG - tig0024068:25332-25351 None:intergenic 25.0%
!! TTTCTTTTGGATTTGGATTT+TGG - tig0024068:25333-25352 None:intergenic 25.0%
! CAAAAGAAAAGACAACAATA+GGG + tig0024068:25344-25363 Msa1413800:CDS 25.0%
!! TTTTTCACTTTGACAACAAT+TGG - tig0024068:25516-25535 None:intergenic 25.0%
! TTCGAACTATCTTTCAAAAT+AGG - tig0024068:25843-25862 None:intergenic 25.0%
! AGAGAAATTGATGAAGAAAA+AGG + tig0024068:25866-25885 Msa1413800:CDS 25.0%
! AAAAAGGATTATGTTACTGA+AGG + tig0024068:25882-25901 Msa1413800:CDS 25.0%
! AAAAGGATTATGTTACTGAA+GGG + tig0024068:25883-25902 Msa1413800:CDS 25.0%
!! ATGCTTAACCATTTAGTTTG+TGG + tig0024068:25264-25283 Msa1413800:CDS 30.0%
TATCATCTTCTAATTGGTGT+TGG - tig0024068:25296-25315 None:intergenic 30.0%
! GTTGTCTTTTCTTTTGGATT+TGG - tig0024068:25340-25359 None:intergenic 30.0%
! CCTATTGTTGTCTTTTCTTT+TGG - tig0024068:25346-25365 None:intergenic 30.0%
CCAAAAGAAAAGACAACAAT+AGG + tig0024068:25343-25362 Msa1413800:CDS 30.0%
AATAATCCTTATGCTTCAAG+AGG + tig0024068:25369-25388 Msa1413800:CDS 30.0%
TTGATCAAGTTCAGATAGAA+GGG - tig0024068:25412-25431 None:intergenic 30.0%
TTTGATCAAGTTCAGATAGA+AGG - tig0024068:25413-25432 None:intergenic 30.0%
TATCTGAACTTGATCAAAGA+AGG + tig0024068:25415-25434 Msa1413800:CDS 30.0%
GAACAAAAGATATGTCATGT+GGG - tig0024068:25464-25483 None:intergenic 30.0%
! TACATCTTTTTCTGAACCAA+TGG + tig0024068:25590-25609 Msa1413800:CDS 30.0%
ATGATGAGATATTTCCAACT+TGG - tig0024068:25646-25665 None:intergenic 30.0%
TCAAACAAGATTGAAAGCTT+TGG + tig0024068:25687-25706 Msa1413800:CDS 30.0%
TCTCTTTGAATATGTTGAAG+AGG + tig0024068:25712-25731 Msa1413800:CDS 30.0%
AAAATCAATATCACAACAGC+AGG - tig0024068:25753-25772 None:intergenic 30.0%
GGAAATTGCCACAAACTAAA+TGG - tig0024068:25275-25294 None:intergenic 35.0%
CGAACAAAAGATATGTCATG+TGG - tig0024068:25465-25484 None:intergenic 35.0%
CAAGAGTGAAGAACAAGTTA+AGG + tig0024068:25557-25576 Msa1413800:CDS 35.0%
! TCAACATGTTTCTGATCCAT+TGG - tig0024068:25609-25628 None:intergenic 35.0%
AGAAACAAAACAAGCCAAGT+TGG + tig0024068:25629-25648 Msa1413800:CDS 35.0%
! TTGTTGTTGTTGACTGTGTT+TGG + tig0024068:25771-25790 Msa1413800:CDS 35.0%
GCTTCTAATGATAAGAGATC+TGG + tig0024068:25933-25952 Msa1413800:CDS 35.0%
AACATAGCCAACAAAAAAGC+TGG + tig0024068:25958-25977 Msa1413800:CDS 35.0%
! TTGGATTTTGGGCTTGAACA+TGG - tig0024068:25321-25340 None:intergenic 40.0%
TCTAAGCCTCTTGAAGCATA+AGG - tig0024068:25378-25397 None:intergenic 40.0%
!! GAAGCTTTAGAATCACCTCT+AGG - tig0024068:25918-25937 None:intergenic 40.0%
ACAGCAGGCACATAGAAAGA+AGG - tig0024068:25738-25757 None:intergenic 45.0%
CGCTTTGTACGTGTATTGTG+TGG + tig0024068:25808-25827 Msa1413800:CDS 45.0%
! GAAGGGTTACTTTCTCCTAG+AGG + tig0024068:25900-25919 Msa1413800:CDS 45.0%
Chromosome Type Strat End Strand Name
tig0024068 gene 25264 25983 25264 ID=Msa1413800;Name=Msa1413800
tig0024068 mRNA 25264 25983 25264 ID=Msa1413800-mRNA-1;Parent=Msa1413800;Name=Msa1413800-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|239
tig0024068 exon 25264 25983 25264 ID=Msa1413800-mRNA-1:exon:21410;Parent=Msa1413800-mRNA-1
tig0024068 CDS 25264 25983 25264 ID=Msa1413800-mRNA-1:cds;Parent=Msa1413800-mRNA-1
Gene Sequence

>Msa1413800

ATGCTTAACCATTTAGTTTGTGGCAATTTCCAACACCAATTAGAAGATGATATACCATGTTCAAGCCCAAAATCCAAATCCAAAAGAAAAGACAACAATAGGGACAATAATCCTTATGCTTCAAGAGGCTTAGATAAATTTTCTACCCTTCTATCTGAACTTGATCAAAGAAGGAAGAAAGTTTACTCACAAATGAACCCACATGACATATCTTTTGTTCGTTTTACATATTCAAGTGATGATTTTGTTCCAATTGTTGTCAAAGTGAAAAATAACAATCATCAGAAGAAACACAAGAGTGAAGAACAAGTTAAGGTAAGACATGTTACATCTTTTTCTGAACCAATGGATCAGAAACATGTTGAAGAAACAAAACAAGCCAAGTTGGAAATATCTCATCATGTTGACGATGTTAAAAATGTATCAAACAAGATTGAAAGCTTTGGATTCTCTTTGAATATGTTGAAGAGGCCTTCTTTCTATGTGCCTGCTGTTGTGATATTGATTTTGTTGTTGTTGACTGTGTTTGGAAGATCATTTGCAACGCTTTGTACGTGTATTGTGTGGTATATAGTTCCTATTTTGAAAGATAGTTCGAAAGAAGAGAAATTGATGAAGAAAAAGGATTATGTTACTGAAGGGTTACTTTCTCCTAGAGGTGATTCTAAAGCTTCTAATGATAAGAGATCTGGAAAACATAGCCAACAAAAAAGCTGGTGA

Protein sequence

>Msa1413800

MLNHLVCGNFQHQLEDDIPCSSPKSKSKRKDNNRDNNPYASRGLDKFSTLLSELDQRRKKVYSQMNPHDISFVRFTYSSDDFVPIVVKVKNNNHQKKHKSEEQVKVRHVTSFSEPMDQKHVEETKQAKLEISHHVDDVKNVSNKIESFGFSLNMLKRPSFYVPAVVILILLLLTVFGRSFATLCTCIVWYIVPILKDSSKEEKLMKKKDYVTEGLLSPRGDSKASNDKRSGKHSQQKSW*