AlfalfaGEDB | Msa0484800

M. sativa cultivar zhongmu-4 / Msa0484800

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010045 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071289 GO:0080090 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000026 GO:2000112 GO:2000280 GO:2001141
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0484800	XP_013470438.1	93.229	192	9	2	1	190	16	205	4.61e-112	328

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0484800	sp\|Q9SLD4\|ZAT11_ARATH	40.341	176	73	4	1	173	17	163	1.19e-29	110

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0484800	A0A072VS27	93.229	192	9	2	1	190	16	205	2.20e-112	328

TFs/TRs:

Gene ID	Type	Classification
Msa0484800	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000820	Msa0484800	0.814371	1.593743e-51	-8.615850e-47
Msa0001380	Msa0484800	0.830384	3.028007e-55	-8.615850e-47
Msa0004480	Msa0484800	0.802156	6.412077e-49	-8.615850e-47
Msa0011050	Msa0484800	0.817059	4.013160e-52	-8.615850e-47
Msa0023060	Msa0484800	0.809077	2.261788e-50	-8.615850e-47
Msa0026630	Msa0484800	0.819154	1.348169e-52	-8.615850e-47
Msa0035020	Msa0484800	0.825716	4.028352e-54	-8.615850e-47
Msa0037760	Msa0484800	0.826648	2.417806e-54	-8.615850e-47
Msa0052360	Msa0484800	0.805344	1.396632e-49	-8.615850e-47
Msa0059900	Msa0484800	0.824314	8.632351e-54	-8.615850e-47
Msa0060070	Msa0484800	0.829715	4.407878e-55	-8.615850e-47
Msa0061220	Msa0484800	0.804884	1.743734e-49	-8.615850e-47
Msa0061500	Msa0484800	0.823685	1.212759e-53	-8.615850e-47
Msa0078150	Msa0484800	0.802343	5.869025e-49	-8.615850e-47
Msa0090380	Msa0484800	0.875170	3.803852e-68	-8.615850e-47
Msa0119430	Msa0484800	0.811996	5.294783e-51	-8.615850e-47
Msa0119440	Msa0484800	0.816147	6.423177e-52	-8.615850e-47
Msa0136630	Msa0484800	0.844597	6.851536e-59	-8.615850e-47
Msa0137860	Msa0484800	0.867928	9.528745e-66	-8.615850e-47
Msa0138900	Msa0484800	0.813595	2.364536e-51	-8.615850e-47
Msa0151220	Msa0484800	0.830088	3.576507e-55	-8.615850e-47
Msa0152560	Msa0484800	0.836837	7.404706e-57	-8.615850e-47
Msa0162690	Msa0484800	0.822267	2.595112e-53	-8.615850e-47
Msa0170770	Msa0484800	0.831146	1.969249e-55	-8.615850e-47
Msa0176260	Msa0484800	0.832425	9.524372e-56	-8.615850e-47
Msa0184000	Msa0484800	0.806462	8.130435e-50	-8.615850e-47
Msa0187310	Msa0484800	0.873631	1.266436e-67	-8.615850e-47
Msa0189610	Msa0484800	0.838387	2.964657e-57	-8.615850e-47
Msa0190930	Msa0484800	0.812419	4.279763e-51	-8.615850e-47
Msa0196880	Msa0484800	0.806173	9.352792e-50	-8.615850e-47
Msa0201570	Msa0484800	0.812922	3.322069e-51	-8.615850e-47
Msa0224130	Msa0484800	0.852873	3.468963e-61	-8.615850e-47
Msa0225830	Msa0484800	0.806978	6.326259e-50	-8.615850e-47
Msa0233250	Msa0484800	0.813496	2.485518e-51	-8.615850e-47
Msa0364790	Msa0484800	0.824963	6.071353e-54	-8.615850e-47
Msa0365950	Msa0484800	0.829915	3.940960e-55	-8.615850e-47
Msa0374870	Msa0484800	0.823221	1.556339e-53	-8.615850e-47
Msa0374890	Msa0484800	0.836150	1.107687e-56	-8.615850e-47
Msa0375070	Msa0484800	0.820517	6.577371e-53	-8.615850e-47
Msa0380600	Msa0484800	0.842656	2.263118e-58	-8.615850e-47
Msa0386010	Msa0484800	0.817192	3.746220e-52	-8.615850e-47
Msa0426560	Msa0484800	0.806609	7.569273e-50	-8.615850e-47
Msa0430240	Msa0484800	0.845838	3.163056e-59	-8.615850e-47
Msa0437070	Msa0484800	0.810350	1.204272e-50	-8.615850e-47
Msa0445630	Msa0484800	0.803006	4.283795e-49	-8.615850e-47
Msa0456770	Msa0484800	0.808157	3.554793e-50	-8.615850e-47
Msa0470880	Msa0484800	0.825429	4.709497e-54	-8.615850e-47
Msa0473140	Msa0484800	0.812191	4.800516e-51	-8.615850e-47
Msa0476680	Msa0484800	0.804503	2.094108e-49	-8.615850e-47
Msa0259440	Msa0484800	0.838700	2.460405e-57	-8.615850e-47
Msa0259580	Msa0484800	0.831299	1.806063e-55	-8.615850e-47
Msa0264680	Msa0484800	0.841198	5.496181e-58	-8.615850e-47
Msa0265270	Msa0484800	0.850085	2.132390e-60	-8.615850e-47
Msa0267790	Msa0484800	0.815999	6.932542e-52	-8.615850e-47
Msa0271550	Msa0484800	0.800133	1.662934e-48	-8.615850e-47
Msa0276430	Msa0484800	0.834494	2.902493e-56	-8.615850e-47
Msa0282720	Msa0484800	0.803531	3.335242e-49	-8.615850e-47
Msa0301170	Msa0484800	0.800756	1.241202e-48	-8.615850e-47
Msa0302730	Msa0484800	0.870218	1.722970e-66	-8.615850e-47
Msa0305620	Msa0484800	0.829340	5.437273e-55	-8.615850e-47
Msa0311510	Msa0484800	0.808342	3.246167e-50	-8.615850e-47
Msa0316470	Msa0484800	0.820404	6.982386e-53	-8.615850e-47
Msa0319240	Msa0484800	0.829588	4.734227e-55	-8.615850e-47
Msa0319290	Msa0484800	0.822966	1.785263e-53	-8.615850e-47
Msa0484800	Msa0500400	0.814403	1.568069e-51	-8.615850e-47
Msa0484800	Msa0521010	0.808550	2.930983e-50	-8.615850e-47
Msa0484800	Msa0524330	0.829913	3.944093e-55	-8.615850e-47
Msa0484800	Msa0524440	0.843412	1.423722e-58	-8.615850e-47
Msa0484800	Msa0534870	0.808278	3.349430e-50	-8.615850e-47
Msa0484800	Msa0545020	0.807651	4.554319e-50	-8.615850e-47
Msa0484800	Msa0545050	0.886286	3.904165e-72	-8.615850e-47
Msa0484800	Msa0558390	0.824272	8.832885e-54	-8.615850e-47
Msa0484800	Msa0573890	0.809698	1.663518e-50	-8.615850e-47
Msa0484800	Msa0574600	0.840491	8.421680e-58	-8.615850e-47
Msa0484800	Msa0584580	0.817088	3.953530e-52	-8.615850e-47
Msa0484800	Msa0584600	0.830444	2.927513e-55	-8.615850e-47
Msa0484800	Msa0588710	0.826878	2.129818e-54	-8.615850e-47
Msa0484800	Msa0590390	0.850790	1.352208e-60	-8.615850e-47
Msa0484800	Msa0600590	0.878991	1.793578e-69	-8.615850e-47
Msa0484800	Msa0613150	0.814562	1.446059e-51	-8.615850e-47
Msa0484800	Msa0613340	0.806359	8.545674e-50	-8.615850e-47
Msa0484800	Msa0615210	0.816636	4.992599e-52	-8.615850e-47
Msa0484800	Msa0628810	0.817251	3.633568e-52	-8.615850e-47
Msa0484800	Msa0631320	0.810395	1.177500e-50	-8.615850e-47
Msa0484800	Msa0635270	0.829000	6.576132e-55	-8.615850e-47
Msa0484800	Msa0641130	0.880851	3.904282e-70	-8.615850e-47
Msa0484800	Msa0646240	0.828108	1.079945e-54	-8.615850e-47
Msa0484800	Msa0654640	0.800228	1.590270e-48	-8.615850e-47
Msa0484800	Msa0662580	0.805899	1.068333e-49	-8.615850e-47
Msa0484800	Msa0669700	0.802045	6.759413e-49	-8.615850e-47
Msa0484800	Msa0679670	0.821897	3.162524e-53	-8.615850e-47
Msa0484800	Msa0696100	0.836165	1.097932e-56	-8.615850e-47
Msa0484800	Msa0697000	0.800309	1.530721e-48	-8.615850e-47
Msa0484800	Msa0697590	0.806760	7.033835e-50	-8.615850e-47
Msa0484800	Msa0708310	0.827764	1.306688e-54	-8.615850e-47
Msa0484800	Msa0734940	0.809726	1.641327e-50	-8.615850e-47
Msa0484800	Msa0743870	0.808259	3.381515e-50	-8.615850e-47
Msa0484800	Msa0744640	0.817688	2.896117e-52	-8.615850e-47
Msa0484800	Msa0748150	0.809515	1.821186e-50	-8.615850e-47
Msa0484800	Msa0752600	0.806135	9.530067e-50	-8.615850e-47
Msa0484800	Msa0769720	0.809040	2.303297e-50	-8.615850e-47
Msa0484800	Msa0770370	0.813693	2.249420e-51	-8.615850e-47
Msa0484800	Msa0772740	0.829241	5.747433e-55	-8.615850e-47
Msa0484800	Msa0787790	0.819673	1.026542e-52	-8.615850e-47
Msa0484800	Msa0807670	0.805538	1.272035e-49	-8.615850e-47
Msa0484800	Msa0812530	0.803902	2.792723e-49	-8.615850e-47
Msa0484800	Msa0833270	0.807611	4.645773e-50	-8.615850e-47
Msa0484800	Msa0841730	0.819107	1.381503e-52	-8.615850e-47
Msa0484800	Msa0860250	0.807727	4.389617e-50	-8.615850e-47
Msa0484800	Msa0863990	0.832350	9.940544e-56	-8.615850e-47
Msa0484800	Msa0870510	0.825543	4.425503e-54	-8.615850e-47
Msa0484800	Msa0896530	0.814223	1.719049e-51	-8.615850e-47
Msa0484800	Msa0945130	0.879945	8.231285e-70	-8.615850e-47
Msa0484800	Msa0968050	0.837636	4.623686e-57	-8.615850e-47
Msa0484800	Msa0994910	0.807301	5.404813e-50	-8.615850e-47
Msa0484800	Msa0996380	0.811268	7.620087e-51	-8.615850e-47
Msa0484800	Msa1022030	0.802457	5.560435e-49	-8.615850e-47
Msa0484800	Msa1023310	0.840484	8.457491e-58	-8.615850e-47
Msa0484800	Msa1030810	0.840244	9.772705e-58	-8.615850e-47
Msa0484800	Msa1043120	0.803843	2.872847e-49	-8.615850e-47
Msa0484800	Msa1062040	0.833432	5.350878e-56	-8.615850e-47
Msa0484800	Msa1069890	0.864447	1.208519e-64	-8.615850e-47
Msa0484800	Msa1089190	0.843904	1.051496e-58	-8.615850e-47
Msa0484800	Msa1102820	0.829276	5.636084e-55	-8.615850e-47
Msa0484800	Msa1107200	0.850319	1.833191e-60	-8.615850e-47
Msa0484800	Msa1119920	0.836750	7.794289e-57	-8.615850e-47
Msa0484800	Msa1123720	0.814497	1.495213e-51	-8.615850e-47
Msa0484800	Msa1131790	0.824512	7.754209e-54	-8.615850e-47
Msa0484800	Msa1150780	0.833460	5.265996e-56	-8.615850e-47
Msa0484800	Msa1157670	0.832202	1.081466e-55	-8.615850e-47
Msa0484800	Msa1180720	0.803854	2.858027e-49	-8.615850e-47
Msa0484800	Msa1183140	0.800077	1.707158e-48	-8.615850e-47
Msa0484800	Msa1187490	0.801789	7.628981e-49	-8.615850e-47
Msa0484800	Msa1208990	0.801897	7.250015e-49	-8.615850e-47
Msa0484800	Msa1212050	0.837143	6.183998e-57	-8.615850e-47
Msa0484800	Msa1212130	0.851522	8.399960e-61	-8.615850e-47
Msa0484800	Msa1214170	0.820065	8.353096e-53	-8.615850e-47
Msa0484800	Msa1218160	0.822637	2.129577e-53	-8.615850e-47
Msa0484800	Msa1225100	0.803540	3.320458e-49	-8.615850e-47
Msa0484800	Msa1225580	0.858178	9.844531e-63	-8.615850e-47
Msa0484800	Msa1231710	0.805350	1.392943e-49	-8.615850e-47
Msa0484800	Msa1236470	0.806005	1.015019e-49	-8.615850e-47
Msa0484800	Msa1246780	0.813612	2.343117e-51	-8.615850e-47
Msa0484800	Msa1246790	0.812282	4.584907e-51	-8.615850e-47
Msa0484800	Msa1256060	0.858051	1.073841e-62	-8.615850e-47
Msa0484800	Msa1259860	0.845550	3.787685e-59	-8.615850e-47
Msa0484800	Msa1260680	0.845880	3.082113e-59	-8.615850e-47
Msa0484800	Msa1280170	0.831078	2.047119e-55	-8.615850e-47
Msa0484800	Msa1286210	0.801389	9.215552e-49	-8.615850e-47
Msa0484800	Msa1286220	0.831809	1.352616e-55	-8.615850e-47
Msa0484800	Msa1296240	0.811749	5.991997e-51	-8.615850e-47
Msa0484800	Msa1309760	0.808675	2.756752e-50	-8.615850e-47
Msa0484800	Msa1312440	0.825921	3.600649e-54	-8.615850e-47
Msa0484800	Msa1337310	0.830670	2.576230e-55	-8.615850e-47
Msa0484800	Msa1338400	0.807626	4.611820e-50	-8.615850e-47
Msa0484800	Msa1353890	0.832698	8.150475e-56	-8.615850e-47
Msa0484800	Msa1355510	0.842103	3.171072e-58	-8.615850e-47
Msa0484800	Msa1355890	0.822786	1.966164e-53	-8.615850e-47
Msa0484800	Msa1360020	0.822965	1.785871e-53	-8.615850e-47
Msa0484800	Msa1364080	0.853388	2.469088e-61	-8.615850e-47
Msa0484800	Msa1367530	0.838924	2.153194e-57	-8.615850e-47
Msa0484800	Msa1369920	-0.819574	1.081511e-52	-8.615850e-47
Msa0484800	Msa1386890	0.849827	2.519024e-60	-8.615850e-47
Msa0484800	Msa1390290	0.800965	1.124901e-48	-8.615850e-47
Msa0484800	Msa1398900	0.805794	1.124102e-49	-8.615850e-47
Msa0484800	Msa1413800	0.837695	4.466166e-57	-8.615850e-47
Msa0484800	Msa1420920	0.816299	5.940502e-52	-8.615850e-47
Msa0484800	Msa1420940	0.812923	3.320863e-51	-8.615850e-47
Msa0484800	Msa1424580	0.809750	1.621836e-50	-8.615850e-47
Msa0484800	Msa1428900	0.822608	2.163146e-53	-8.615850e-47
Msa0484800	Msa1433410	0.876510	1.319140e-68	-8.615850e-47
Msa0484800	Msa1436090	0.837026	6.627321e-57	-8.615850e-47
Msa0484800	Msa1443620	0.861224	1.192540e-63	-8.615850e-47
Msa0484800	Msa1443640	0.862513	4.804728e-64	-8.615850e-47
Msa0484800	Msa1461410	0.800921	1.148772e-48	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0484800	MtrunA17_Chr1g0212561	93.229	192	9	2	1	190	16	205	4.24e-116	328
Msa0484800	MtrunA17_Chr7g0222831	55.851	188	65	5	1	186	12	183	1.09e-49	159
Msa0484800	MtrunA17_Chr1g0182161	44.509	173	66	6	1	173	16	158	1.92e-33	117
Msa0484800	MtrunA17_Chr7g0263131	39.326	178	73	3	2	179	15	157	4.49e-32	113
Msa0484800	MtrunA17_Chr1g0182181	40.230	174	72	4	1	173	11	153	7.59e-31	110
Msa0484800	MtrunA17_Chr7g0263121	37.572	173	81	3	1	173	15	160	1.82e-27	102
Msa0484800	MtrunA17_Chr2g0327901	40.606	165	45	4	5	168	14	126	4.74e-27	99.8
Msa0484800	MtrunA17_Chr1g0197281	38.372	172	57	4	29	171	155	306	4.26e-24	97.1
Msa0484800	MtrunA17_Chr1g0180311	46.018	113	29	3	20	102	112	222	1.34e-20	86.7
Msa0484800	MtrunA17_Chr4g0027991	39.167	120	27	2	29	102	112	231	2.48e-19	83.2
Msa0484800	MtrunA17_Chr1g0188911	37.288	118	34	3	22	101	38	153	4.88e-15	70.1
Msa0484800	MtrunA17_Chr4g0012691	44.444	81	36	2	30	101	220	300	6.29e-14	68.9
Msa0484800	MtrunA17_Chr4g0012791	39.450	109	30	3	29	101	226	334	1.49e-13	68.2
Msa0484800	MtrunA17_Chr7g0231181	43.902	82	35	3	26	101	232	308	1.66e-13	67.8
Msa0484800	MtrunA17_Chr1g0207451	46.479	71	37	1	31	101	84	153	1.02e-12	64.7
Msa0484800	MtrunA17_Chr3g0134711	47.436	78	34	1	31	101	87	164	1.10e-11	61.6
Msa0484800	MtrunA17_Chr1g0188901	41.379	87	37	3	29	101	59	145	1.20e-11	60.8
Msa0484800	MtrunA17_Chr1g0152661	41.772	79	39	1	30	101	81	159	5.19e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0484800	AT2G37430.1	40.341	176	73	4	1	173	17	163	1.21e-30	110
Msa0484800	AT5G59820.1	42.045	176	70	5	2	177	16	159	4.02e-29	106
Msa0484800	AT3G46080.1	42.135	178	60	7	2	172	16	157	1.98e-28	104
Msa0484800	AT2G28710.1	37.853	177	73	4	1	177	14	153	2.59e-28	103
Msa0484800	AT3G46090.1	40.449	178	73	7	2	177	17	163	2.03e-26	99.4
Msa0484800	AT2G28200.1	34.934	229	84	8	1	185	77	284	2.76e-26	102
Msa0484800	AT3G46070.1	43.023	172	63	8	2	168	16	157	4.77e-26	98.6
Msa0484800	AT3G53600.1	39.735	151	58	4	23	173	43	160	3.07e-25	96.7
Msa0484800	AT5G04390.1	47.525	101	26	2	29	102	152	252	9.77e-21	88.2
Msa0484800	AT5G03510.1	47.475	99	27	2	29	102	117	215	2.03e-20	86.7
Msa0484800	AT3G10470.1	36.628	172	78	4	29	169	181	352	6.84e-20	86.3
Msa0484800	AT5G43170.1	44.118	102	45	5	1	101	50	140	1.06e-16	74.7
Msa0484800	AT1G02030.1	48.101	79	33	2	27	101	157	231	2.50e-15	72.4
Msa0484800	AT2G45120.1	42.353	85	49	0	17	101	181	265	4.48e-15	72.4
Msa0484800	AT3G60580.1	44.737	76	36	2	30	101	173	246	9.88e-15	70.9
Msa0484800	AT3G19580.1	42.353	85	39	3	27	101	103	187	2.85e-14	69.7
Msa0484800	AT3G19580.2	42.353	85	39	3	27	101	103	187	2.85e-14	69.7
Msa0484800	AT1G49900.1	46.575	73	36	2	30	101	750	820	6.10e-14	69.7
Msa0484800	AT1G49900.1	44.872	78	36	3	29	101	192	267	8.81e-13	66.6
Msa0484800	AT4G35280.1	39.496	119	54	4	30	134	162	276	1.21e-13	67.8
Msa0484800	AT5G67450.1	38.776	98	42	2	22	101	89	186	2.76e-13	66.6
Msa0484800	AT2G17180.1	31.788	151	75	5	1	135	125	263	2.00e-12	64.3
Msa0484800	AT5G04340.1	39.759	83	40	1	29	101	88	170	6.98e-12	62.4
Msa0484800	AT3G49930.1	36.449	107	58	3	5	101	65	171	9.47e-12	62.0
Msa0484800	AT1G27730.1	44.304	79	37	2	30	101	80	158	5.19e-11	60.1
Msa0484800	AT5G56200.1	30.872	149	71	4	32	160	344	480	6.16e-11	60.8

Find 29 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCATTTGTGGATTAGAATT+TGG	0.159190	3_4:+2316398	Msa0484800:CDS
TGGACAACCAACTCTTAATT+TGG	0.245604	3_4:+2316679	Msa0484800:CDS
AAATACTTGATGTTACTTTC+TGG	0.260688	3_4:+2316161	Msa0484800:CDS
ATTTGGAATAGGACAAGCTT+TGG	0.376115	3_4:+2316415	Msa0484800:CDS
ACGCATGCATGTTCCATTTG+TGG	0.385337	3_4:+2316386	Msa0484800:CDS
TGTGGATTAGAATTTGGAAT+AGG	0.387282	3_4:+2316404	Msa0484800:CDS
TGACACCTTTAGAGAATGAT+TGG	0.404566	3_4:+2316642	Msa0484800:CDS
CTCAAAGCTAATAATTCAAA+TGG	0.409329	3_4:+2316479	Msa0484800:CDS
TCCAAATTCTAATCCACAAA+TGG	0.431756	3_4:-2316399	None:intergenic
ACAACCAACTCTTAATTTGG+TGG	0.451155	3_4:+2316682	Msa0484800:CDS
TTTGGAATAGGACAAGCTTT+GGG	0.459019	3_4:+2316416	Msa0484800:CDS
TCACAAGAAACCAAGGTTGA+TGG	0.462801	3_4:+2316307	Msa0484800:CDS
AACTTCCAATCATTCTCTAA+AGG	0.507967	3_4:-2316647	None:intergenic
TACTTGATGTTACTTTCTGG+TGG	0.513625	3_4:+2316164	Msa0484800:CDS
ACTACTAGTAGTAGCAATAG+TGG	0.516156	3_4:+2316530	Msa0484800:CDS
CTAGTCATCTCCATCAACCT+TGG	0.544532	3_4:-2316317	None:intergenic
CAATCCACCAAATTAAGAGT+TGG	0.550722	3_4:-2316686	None:intergenic
TTGATGTTACTTTCTGGTGG+AGG	0.550811	3_4:+2316167	Msa0484800:CDS
TAACATCAAGTATTTAGCCA+TGG	0.579745	3_4:-2316153	None:intergenic
GATTCAGCAAAGAGGAGCAA+GGG	0.587011	3_4:+2316584	Msa0484800:CDS
AAGCTTTGGGTGGTCACATG+AGG	0.610856	3_4:+2316429	Msa0484800:CDS
TCATCGTTTCAAGCGTTGGG+AGG	0.614601	3_4:+2316272	Msa0484800:CDS
CTAATACTGATTCAGCAAAG+AGG	0.617636	3_4:+2316576	Msa0484800:CDS
TGATTCAGCAAAGAGGAGCA+AGG	0.619501	3_4:+2316583	Msa0484800:CDS
GGAATAGGACAAGCTTTGGG+TGG	0.625426	3_4:+2316419	Msa0484800:CDS
ATTCAGCAAAGAGGAGCAAG+GGG	0.654634	3_4:+2316585	Msa0484800:CDS
GTGCGAGTCACAAGAAACCA+AGG	0.659856	3_4:+2316300	Msa0484800:CDS
ATGTCTTGCACTCAAACACA+CGG	0.662690	3_4:-2316235	None:intergenic
ATGACTAGTGATGATCAACA+TGG	0.726427	3_4:+2316332	Msa0484800:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATACTTGATGTTACTTTC+TGG	+	chr3_4:2316161-2316180	Msa0484800:CDS	25.0%
!	CTCAAAGCTAATAATTCAAA+TGG	+	chr3_4:2316479-2316498	Msa0484800:CDS	25.0%
!!	GAATGATTGGAAGTTTTTAA+AGG	+	chr3_4:2316655-2316674	Msa0484800:CDS	25.0%
!!	TCCATTTGTGGATTAGAATT+TGG	+	chr3_4:2316398-2316417	Msa0484800:CDS	30.0%
	TCCAAATTCTAATCCACAAA+TGG	-	chr3_4:2316402-2316421	None:intergenic	30.0%
!!	TGTGGATTAGAATTTGGAAT+AGG	+	chr3_4:2316404-2316423	Msa0484800:CDS	30.0%
!!	CAACAAGAGATTTTTGTTTC+TGG	+	chr3_4:2316610-2316629	Msa0484800:CDS	30.0%
	AACTTCCAATCATTCTCTAA+AGG	-	chr3_4:2316650-2316669	None:intergenic	30.0%
!!	GATTGGAAGTTTTTAAAGGT+TGG	+	chr3_4:2316659-2316678	Msa0484800:CDS	30.0%
!!	TACTTGATGTTACTTTCTGG+TGG	+	chr3_4:2316164-2316183	Msa0484800:CDS	35.0%
!	TTTTCATCGTTTCAAGCGTT+GGG	+	chr3_4:2316269-2316288	Msa0484800:CDS	35.0%
	ATGACTAGTGATGATCAACA+TGG	+	chr3_4:2316332-2316351	Msa0484800:CDS	35.0%
!	ATTTGGAATAGGACAAGCTT+TGG	+	chr3_4:2316415-2316434	Msa0484800:CDS	35.0%
!	TTTGGAATAGGACAAGCTTT+GGG	+	chr3_4:2316416-2316435	Msa0484800:CDS	35.0%
	ACTACTAGTAGTAGCAATAG+TGG	+	chr3_4:2316530-2316549	Msa0484800:CDS	35.0%
!!	CTAATACTGATTCAGCAAAG+AGG	+	chr3_4:2316576-2316595	Msa0484800:CDS	35.0%
!	TGACACCTTTAGAGAATGAT+TGG	+	chr3_4:2316642-2316661	Msa0484800:CDS	35.0%
	TGGACAACCAACTCTTAATT+TGG	+	chr3_4:2316679-2316698	Msa0484800:CDS	35.0%
!	ACAACCAACTCTTAATTTGG+TGG	+	chr3_4:2316682-2316701	Msa0484800:CDS	35.0%
	CAATCCACCAAATTAAGAGT+TGG	-	chr3_4:2316689-2316708	None:intergenic	35.0%
!!	TTGATGTTACTTTCTGGTGG+AGG	+	chr3_4:2316167-2316186	Msa0484800:CDS	40.0%
	ATGTCTTGCACTCAAACACA+CGG	-	chr3_4:2316238-2316257	None:intergenic	40.0%
!	GTTTTCATCGTTTCAAGCGT+TGG	+	chr3_4:2316268-2316287	Msa0484800:CDS	40.0%
	TCACAAGAAACCAAGGTTGA+TGG	+	chr3_4:2316307-2316326	Msa0484800:CDS	40.0%
	CTAGTCATCTCCATCAACCT+TGG	-	chr3_4:2316320-2316339	None:intergenic	45.0%
!!	TGCGTTTTAGTCTTCGTCGT+TGG	-	chr3_4:2316371-2316390	None:intergenic	45.0%
	ACGCATGCATGTTCCATTTG+TGG	+	chr3_4:2316386-2316405	Msa0484800:CDS	45.0%
	TGATTCAGCAAAGAGGAGCA+AGG	+	chr3_4:2316583-2316602	Msa0484800:CDS	45.0%
	GATTCAGCAAAGAGGAGCAA+GGG	+	chr3_4:2316584-2316603	Msa0484800:CDS	45.0%
	ATTCAGCAAAGAGGAGCAAG+GGG	+	chr3_4:2316585-2316604	Msa0484800:CDS	45.0%
!!	TCATCGTTTCAAGCGTTGGG+AGG	+	chr3_4:2316272-2316291	Msa0484800:CDS	50.0%
	GTGCGAGTCACAAGAAACCA+AGG	+	chr3_4:2316300-2316319	Msa0484800:CDS	50.0%
!!	GGAATAGGACAAGCTTTGGG+TGG	+	chr3_4:2316419-2316438	Msa0484800:CDS	50.0%
!!	AAGCTTTGGGTGGTCACATG+AGG	+	chr3_4:2316429-2316448	Msa0484800:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	2316155	2316727	2316155	ID=Msa0484800;Name=Msa0484800
chr3_4	mRNA	2316155	2316727	2316155	ID=Msa0484800-mRNA-1;Parent=Msa0484800;Name=Msa0484800-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|190
chr3_4	exon	2316155	2316727	2316155	ID=Msa0484800-mRNA-1:exon:799;Parent=Msa0484800-mRNA-1
chr3_4	CDS	2316155	2316727	2316155	ID=Msa0484800-mRNA-1:cds;Parent=Msa0484800-mRNA-1

Gene Sequence

>Msa0484800

ATGGCTAAATACTTGATGTTACTTTCTGGTGGAGGTGATAAAATATTTGATCAAGTGAATTACTCTTCAAATTTCAACAACCGTGTGTTTGAGTGCAAGACATGTAAAAGACAGTTTTCATCGTTTCAAGCGTTGGGAGGACATCGTGCGAGTCACAAGAAACCAAGGTTGATGGAGATGACTAGTGATGATCAACATGGTTCAATACTTACACCAACGACGAAGACTAAAACGCATGCATGTTCCATTTGTGGATTAGAATTTGGAATAGGACAAGCTTTGGGTGGTCACATGAGGCGACACAGAAGAACAGAATCATCAAAACTCAAAGCTAATAATTCAAATGGAAACATGCATAATTTTATGACTACTACAACTACTAGTAGTAGCAATAGTGGTTGCAGTACTATTGATAATAGTACTAATACTGATTCAGCAAAGAGGAGCAAGGGGAACAACAAGAGATTTTTGTTTCTGGATTTGAATTTGACACCTTTAGAGAATGATTGGAAGTTTTTAAAGGTTGGACAACCAACTCTTAATTTGGTGGATTGTTTCAATTCAATTCATTAA

Protein sequence

>Msa0484800

MAKYLMLLSGGGDKIFDQVNYSSNFNNRVFECKTCKRQFSSFQALGGHRASHKKPRLMEMTSDDQHGSILTPTTKTKTHACSICGLEFGIGQALGGHMRRHRRTESSKLKANNSNGNMHNFMTTTTTSSSNSGCSTIDNSTNTDSAKRSKGNNKRFLFLDLNLTPLENDWKFLKVGQPTLNLVDCFNSIH*