AlfalfaGEDB | Msa0087140

M. sativa cultivar zhongmu-4 / Msa0087140

KEGG: K07560|K11644
GO: GO:0000003 GO:0001708 GO:0002119 GO:0002164 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0007423 GO:0007548 GO:0008150 GO:0009653 GO:0009791 GO:0009887 GO:0009987 GO:0022414 GO:0030154 GO:0030539 GO:0032501 GO:0032502 GO:0035112 GO:0040011 GO:0040025 GO:0045165 GO:0046661 GO:0048513 GO:0048569 GO:0048608 GO:0048731 GO:0048806 GO:0048808 GO:0048856 GO:0048869 GO:0061458 GO:0090596 GO:0090597 GO:0090598
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087140	A0A396JTY0	80.892	157	20	2	1	147	23	179	1.38e-83	252

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa0087140	0.808354	3.228198e-50	-8.615850e-47
Msa0087140	Msa0153910	0.812662	3.786642e-51	-8.615850e-47
Msa0087140	Msa0157290	0.808547	2.935897e-50	-8.615850e-47
Msa0087140	Msa0258760	0.813899	2.026203e-51	-8.615850e-47
Msa0087140	Msa0323080	0.801161	1.026172e-48	-8.615850e-47
Msa0087140	Msa0428930	0.805301	1.426206e-49	-8.615850e-47
Msa0087140	Msa0431690	0.809964	1.458227e-50	-8.615850e-47
Msa0087140	Msa0437990	0.800543	1.371611e-48	-8.615850e-47
Msa0087140	Msa0599890	0.803906	2.787643e-49	-8.615850e-47
Msa0087140	Msa0832370	0.802644	5.087337e-49	-8.615850e-47
Msa0087140	Msa0844900	0.800830	1.198977e-48	-8.615850e-47
Msa0087140	Msa0880140	0.806041	9.970904e-50	-8.615850e-47
Msa0087140	Msa0885280	0.804408	2.191947e-49	-8.615850e-47
Msa0087140	Msa0885290	0.801937	7.111978e-49	-8.615850e-47
Msa0087140	Msa0897880	0.802343	5.868135e-49	-8.615850e-47
Msa0087140	Msa0907070	0.808148	3.570573e-50	-8.615850e-47
Msa0087140	Msa0975220	0.801955	7.052772e-49	-8.615850e-47
Msa0087140	Msa1014540	0.815531	8.812975e-52	-8.615850e-47
Msa0087140	Msa1075220	-0.802053	6.732942e-49	-8.615850e-47
Msa0087140	Msa1082500	0.813581	2.381295e-51	-8.615850e-47
Msa0087140	Msa1136090	0.803406	3.540164e-49	-8.615850e-47
Msa0087140	Msa1162550	-0.801018	1.097253e-48	-8.615850e-47
Msa0087140	Msa1199220	0.801158	1.027191e-48	-8.615850e-47
Msa0087140	Msa1205130	0.820904	5.359974e-53	-8.615850e-47
Msa0087140	Msa1233500	0.805451	1.326643e-49	-8.615850e-47
Msa0087140	Msa1244250	0.802053	6.733812e-49	-8.615850e-47
Msa0087140	Msa1284680	0.816138	6.454860e-52	-8.615850e-47
Msa0087140	Msa1288000	0.804857	1.766109e-49	-8.615850e-47
Msa0087140	Msa1318990	0.804696	1.908300e-49	-8.615850e-47
Msa0087140	Msa1324780	0.813872	2.054513e-51	-8.615850e-47
Msa0087140	Msa1359040	0.805467	1.316106e-49	-8.615850e-47
Msa0087140	Msa1464940	0.807586	4.702650e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087140	MtrunA17_Chr1g0202841	80.892	157	20	2	1	147	23	179	2.66e-87	252
Msa0087140	MtrunA17_Chr3g0093911	75.484	155	30	1	1	147	196	350	3.66e-76	230
Msa0087140	MtrunA17_Chr1g0207351	67.532	154	42	1	2	147	213	366	2.21e-63	197
Msa0087140	MtrunA17_Chr7g0252101	64.286	154	47	2	2	147	279	432	4.77e-59	188
Msa0087140	MtrunA17_Chr1g0209051	62.745	153	49	1	2	146	118	270	6.12e-59	183
Msa0087140	MtrunA17_Chr3g0077801	55.195	154	55	4	2	144	199	349	6.61e-43	145
Msa0087140	MtrunA17_Chr4g0075971	53.797	158	55	5	2	147	451	602	4.11e-41	144
Msa0087140	MtrunA17_Chr3g0077851	51.899	158	58	5	2	146	183	335	4.51e-38	132
Msa0087140	MtrunA17_Chr7g0243331	49.045	157	65	5	2	147	198	350	5.95e-37	129
Msa0087140	MtrunA17_Chr6g0488101	45.783	166	67	5	2	147	293	455	8.30e-36	128
Msa0087140	MtrunA17_Chr3g0077901	47.771	157	64	4	3	146	220	371	3.06e-35	125
Msa0087140	MtrunA17_Chr6g0488271	45.181	166	68	5	2	147	253	415	5.17e-35	125
Msa0087140	MtrunA17_Chr3g0112311	46.012	163	66	5	2	145	196	355	8.10e-35	124
Msa0087140	MtrunA17_Chr1g0196241	67.033	91	26	2	42	131	1	88	2.99e-34	115
Msa0087140	MtrunA17_Chr1g0209191	59.406	101	31	2	53	146	1	98	8.74e-34	114
Msa0087140	MtrunA17_Chr6g0452641	45.509	167	66	5	2	147	178	340	2.64e-33	119
Msa0087140	MtrunA17_Chr6g0486591	44.872	156	73	4	2	147	7	159	3.03e-33	114
Msa0087140	MtrunA17_Chr3g0123471	47.333	150	63	4	2	139	114	259	6.30e-33	116
Msa0087140	MtrunA17_Chr3g0122991	48.092	131	47	3	1	113	147	274	2.56e-30	110
Msa0087140	MtrunA17_Chr1g0187941	51.754	114	47	1	2	107	24	137	4.64e-30	105
Msa0087140	MtrunA17_Chr6g0459061	33.071	127	75	4	23	144	234	355	2.81e-13	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAATATGTAATGTGTATTC+AGG	0.241561	1_2:-67950469	Msa0087140:CDS
CGTCACAATTAACCAACTTT+AGG	0.304358	1_2:+67950704	None:intergenic
CACTCATATCCCCATCTATT+CGG	0.337148	1_2:+67950523	None:intergenic
TTCCAGTCTGACGTAATTCA+TGG	0.341057	1_2:+67950615	None:intergenic
CATTGAGCAATCCGAATAGA+TGG	0.371315	1_2:-67950534	Msa0087140:CDS
AGCAAACACATTGGTGCATC+AGG	0.395473	1_2:+67950406	None:intergenic
CAATGTGTTTGCTGCTCGAT+TGG	0.450116	1_2:-67950396	Msa0087140:CDS
GTACACATTACTTATTTCGT+TGG	0.455167	1_2:+67950654	None:intergenic
TTACGTCAGACTGGAAACTT+TGG	0.463157	1_2:-67950608	Msa0087140:CDS
ATGGCCAACATAGGGGTACT+TGG	0.477796	1_2:-67950792	Msa0087140:CDS
AATTAACCAACTTTAGGTAC+GGG	0.478189	1_2:+67950710	None:intergenic
CACATTGGTGCATCAGGAGC+TGG	0.485059	1_2:+67950412	None:intergenic
TACGTCAGACTGGAAACTTT+GGG	0.491472	1_2:-67950607	Msa0087140:CDS
CAATATGTAATGTGTATTCA+GGG	0.491526	1_2:-67950468	Msa0087140:CDS
CAATTAACCAACTTTAGGTA+CGG	0.499252	1_2:+67950709	None:intergenic
TCACCAACATGGCCAACATA+GGG	0.519186	1_2:-67950800	None:intergenic
AACCATGAATTACGTCAGAC+TGG	0.538409	1_2:-67950617	Msa0087140:CDS
GTCACCAACATGGCCAACAT+AGG	0.563679	1_2:-67950801	None:intergenic
CAAGCCAAGTACCCCTATGT+TGG	0.572197	1_2:+67950788	None:intergenic
ATTGAGCAATCCGAATAGAT+GGG	0.573573	1_2:-67950533	Msa0087140:CDS
GGTACGGGGAAAATTTACAC+TGG	0.588387	1_2:+67950725	None:intergenic
CAATCGAGCAGCAAACACAT+TGG	0.626063	1_2:+67950397	None:intergenic
GTACGGGGAAAATTTACACT+GGG	0.651032	1_2:+67950726	None:intergenic
CACCAACATGGCCAACATAG+GGG	0.652462	1_2:-67950799	None:intergenic
TTGAGCAATCCGAATAGATG+GGG	0.667711	1_2:-67950532	Msa0087140:CDS
ATTAACCAACTTTAGGTACG+GGG	0.670149	1_2:+67950711	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACAATATGTAATGTGTATTC+AGG	-	chr1_2:67950671-67950690	Msa0087140:CDS	25.0%
!	CAATATGTAATGTGTATTCA+GGG	-	chr1_2:67950672-67950691	Msa0087140:CDS	25.0%
	AATTAACCAACTTTAGGTAC+GGG	+	chr1_2:67950433-67950452	None:intergenic	30.0%
	CAATTAACCAACTTTAGGTA+CGG	+	chr1_2:67950434-67950453	None:intergenic	30.0%
	GTACACATTACTTATTTCGT+TGG	+	chr1_2:67950489-67950508	None:intergenic	30.0%
	ATTAACCAACTTTAGGTACG+GGG	+	chr1_2:67950432-67950451	None:intergenic	35.0%
	CGTCACAATTAACCAACTTT+AGG	+	chr1_2:67950439-67950458	None:intergenic	35.0%
!!	GGTGACATTGCATTATTTTG+TGG	-	chr1_2:67950554-67950573	Msa0087140:CDS	35.0%
	ATTGAGCAATCCGAATAGAT+GGG	-	chr1_2:67950607-67950626	Msa0087140:CDS	35.0%
	GTACGGGGAAAATTTACACT+GGG	+	chr1_2:67950417-67950436	None:intergenic	40.0%
!	ATTTTCCCCGTACCTAAAGT+TGG	-	chr1_2:67950424-67950443	Msa0087140:CDS	40.0%
!	TTGTGACGAGCAACTTTTAG+TGG	-	chr1_2:67950451-67950470	Msa0087140:CDS	40.0%
	AACCATGAATTACGTCAGAC+TGG	-	chr1_2:67950523-67950542	Msa0087140:CDS	40.0%
	TTCCAGTCTGACGTAATTCA+TGG	+	chr1_2:67950528-67950547	None:intergenic	40.0%
	TTACGTCAGACTGGAAACTT+TGG	-	chr1_2:67950532-67950551	Msa0087140:CDS	40.0%
	TACGTCAGACTGGAAACTTT+GGG	-	chr1_2:67950533-67950552	Msa0087140:CDS	40.0%
	CATTGAGCAATCCGAATAGA+TGG	-	chr1_2:67950606-67950625	Msa0087140:CDS	40.0%
	TTGAGCAATCCGAATAGATG+GGG	-	chr1_2:67950608-67950627	Msa0087140:CDS	40.0%
	CACTCATATCCCCATCTATT+CGG	+	chr1_2:67950620-67950639	None:intergenic	40.0%
	GGTACGGGGAAAATTTACAC+TGG	+	chr1_2:67950418-67950437	None:intergenic	45.0%
	AGCAAACACATTGGTGCATC+AGG	+	chr1_2:67950737-67950756	None:intergenic	45.0%
	CAATCGAGCAGCAAACACAT+TGG	+	chr1_2:67950746-67950765	None:intergenic	45.0%
!	CAATGTGTTTGCTGCTCGAT+TGG	-	chr1_2:67950744-67950763	Msa0087140:intron	45.0%
!!	ATGGCCAACATAGGGGTACT+TGG	-	chr1_2:67950348-67950367	Msa0087140:CDS	50.0%
	CAAGCCAAGTACCCCTATGT+TGG	+	chr1_2:67950355-67950374	None:intergenic	50.0%
	CACATTGGTGCATCAGGAGC+TGG	+	chr1_2:67950731-67950750	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	67950348	67950814	67950348	ID=Msa0087140;Name=Msa0087140
chr1_2	mRNA	67950348	67950814	67950348	ID=Msa0087140-mRNA-1;Parent=Msa0087140;Name=Msa0087140-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|0\|0\|1\|0\|0\|2\|0\|147
chr1_2	exon	67950776	67950814	67950776	ID=Msa0087140-mRNA-1:exon:23155;Parent=Msa0087140-mRNA-1
chr1_2	exon	67950348	67950752	67950348	ID=Msa0087140-mRNA-1:exon:23154;Parent=Msa0087140-mRNA-1
chr1_2	CDS	67950776	67950814	67950776	ID=Msa0087140-mRNA-1:cds;Parent=Msa0087140-mRNA-1
chr1_2	CDS	67950348	67950752	67950348	ID=Msa0087140-mRNA-1:cds;Parent=Msa0087140-mRNA-1

Gene Sequence

>Msa0087140

ATGGCCAACATAGGGGTACTTGGCTTGAATTCTGCAAATTACTCCCAGTGTAAATTTTCCCCGTACCTAAAGTTGGTTAATTGTGACGAGCAACTTTTAGTGGTTGATTTTACGTCCAACGAAATAAGTAATGTGTACAAGATAGACTTCTCAACCATGAATTACGTCAGACTGGAAACTTTGGGTGACATTGCATTATTTTGTGGTTTGAATTGTTTGAAGAGAAACTGTTATGCATTGAGCAATCCGAATAGATGGGGATATGAGTGCAATTCTGTGTATGACATCAGTCTTTTATCTACAATATGTAATGTGTATTCAGGGAATGATAAAAAATTGCAAAAAAGCATTACGCTTCCAGCTCCTGATGCACCAATGTGTTTGCTGCTCGATTGGTGTTTTAGACATCAACAATATGAGATAGATTATTCTCTAGTTGAGTAA

Protein sequence

>Msa0087140

MANIGVLGLNSANYSQCKFSPYLKLVNCDEQLLVVDFTSNEISNVYKIDFSTMNYVRLETLGDIALFCGLNCLKRNCYALSNPNRWGYECNSVYDISLLSTICNVYSGNDKKLQKSITLPAPDAPMCLLLDWCFRHQQYEIDYSLVE*