AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0089150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATGAAATTTCTTATAA+AGG 0.286353 1_2:+70575881 None:intergenic
ACCGACCAATCCTGTGAATT+AGG 0.346868 1_2:+70575930 None:intergenic
TACCCGGCGCCGGAGACTTA+TGG 0.351875 1_2:-70577677 Msa0089150:CDS
GAGAAGAGAAAGTTGGAAAT+GGG 0.352471 1_2:+70577908 None:intergenic
AGACATTACTTTGCACAGAT+TGG 0.375088 1_2:-70576079 Msa0089150:three_prime_UTR
CCACCTCGTCAGCAAAATTC+AGG 0.380629 1_2:-70577527 Msa0089150:CDS
ACAAGGTTACCCTCCACAAC+AGG 0.383576 1_2:-70577621 Msa0089150:CDS
GAATAGAGGATGGACCTTGC+TGG 0.394206 1_2:+70577818 None:intergenic
AGAGAAGAGAAAGTTGGAAA+TGG 0.398970 1_2:+70577907 None:intergenic
TGGTTGTAGTAACTCATTGT+GGG 0.411137 1_2:+70577868 None:intergenic
TCTCTGCTGTTGCTGTCTCT+TGG 0.422721 1_2:-70576118 Msa0089150:CDS
CAGCAAAATTCAGGCGGTGC+TGG 0.441154 1_2:-70577518 Msa0089150:CDS
TTTCCATAAGTCTCCGGCGC+CGG 0.444590 1_2:+70577674 None:intergenic
TTCCATAAGTCTCCGGCGCC+GGG 0.453331 1_2:+70577675 None:intergenic
AATAGAGGATGGACCTTGCT+GGG 0.456352 1_2:+70577819 None:intergenic
CGCCGACGGGCGGTTGCTGT+TGG 0.456584 1_2:+70577848 None:intergenic
TTTCGTACTGCCTAATTCAC+AGG 0.460541 1_2:-70575940 Msa0089150:three_prime_UTR
CATGGAATTAATAGGTTACC+CGG 0.471275 1_2:-70577693 Msa0089150:intron
GTGCTGGTTGCATGGAAGGC+TGG 0.477733 1_2:-70577502 Msa0089150:intron
GGCGGTGCTGGTTGCATGGA+AGG 0.485634 1_2:-70577506 Msa0089150:CDS
TAGTGGAGAGAAGAGAAAGT+TGG 0.493429 1_2:+70577901 None:intergenic
TACTGCCTAATTCACAGGAT+TGG 0.494829 1_2:-70575935 Msa0089150:three_prime_UTR
TAACCTTGTGGAGGATATCC+TGG 0.496681 1_2:+70577635 None:intergenic
GAATTAGGCAGTACGAAAGT+TGG 0.503589 1_2:+70575945 None:intergenic
TTCAGGCGGTGCTGGTTGCA+TGG 0.511069 1_2:-70577510 Msa0089150:CDS
TGCTGGGGAGGAACGCCGAC+GGG 0.513380 1_2:+70577835 None:intergenic
GCCTAATTCACAGGATTGGT+CGG 0.530936 1_2:-70575931 Msa0089150:three_prime_UTR
AAGGTAACAAGCATCACAAA+AGG 0.532560 1_2:+70575900 None:intergenic
GTTGTAGTAACTCATTGTGG+GGG 0.541005 1_2:+70577870 None:intergenic
GGTTGTAGTAACTCATTGTG+GGG 0.550225 1_2:+70577869 None:intergenic
AGAAGAGAAAGTTGGAAATG+GGG 0.551961 1_2:+70577909 None:intergenic
TGCGGTGGGTAGCCTTGTTG+TGG 0.552033 1_2:+70577575 None:intergenic
GAGGATGGACCTTGCTGGGG+AGG 0.552104 1_2:+70577823 None:intergenic
TTGCTGGGGAGGAACGCCGA+CGG 0.554888 1_2:+70577834 None:intergenic
TGTGGAGGATATCCTGGTGG+AGG 0.557895 1_2:+70577641 None:intergenic
TGAGGTGGATAGCCCTGTTG+TGG 0.565061 1_2:+70577608 None:intergenic
ATTAATAGGTTACCCGGCGC+CGG 0.567543 1_2:-70577687 Msa0089150:intron
TTGGTTGTAGTAACTCATTG+TGG 0.572996 1_2:+70577867 None:intergenic
GTCGGCGTTCCTCCCCAGCA+AGG 0.585619 1_2:-70577832 Msa0089150:intron
TGGGGAGGAACGCCGACGGG+CGG 0.586625 1_2:+70577838 None:intergenic
TTCCGCAAAACAAAACAGAT+CGG 0.587583 1_2:-70577948 None:intergenic
GGTGGATAGCCCTGTTGTGG+AGG 0.592582 1_2:+70577611 None:intergenic
TACTGAGGAGCATATTGCGG+TGG 0.605867 1_2:+70577560 None:intergenic
AACCAACAGCAACCGCCCGT+CGG 0.612199 1_2:-70577850 Msa0089150:CDS
TTGTAGTAACTCATTGTGGG+GGG 0.615172 1_2:+70577871 None:intergenic
GTGGAGGATATCCTGGTGGA+GGG 0.625281 1_2:+70577642 None:intergenic
CAAGGCTATCCTCCACAACA+AGG 0.626755 1_2:-70577587 Msa0089150:CDS
GGTGGGTAGCCTTGTTGTGG+AGG 0.627143 1_2:+70577578 None:intergenic
AAAGATGCTTACCCTCCACC+AGG 0.636903 1_2:-70577653 Msa0089150:CDS
CAACAGGGCTATCCACCTCA+AGG 0.638659 1_2:-70577605 Msa0089150:CDS
GGTGGTGGCTGAGCGTACTG+AGG 0.643562 1_2:+70577545 None:intergenic
ACTGAGGAGCATATTGCGGT+GGG 0.646934 1_2:+70577561 None:intergenic
TGTTGTGGAGGGTAACCTTG+TGG 0.649835 1_2:+70577623 None:intergenic
ATAGAGGATGGACCTTGCTG+GGG 0.654237 1_2:+70577820 None:intergenic
GTGGATAGCCCTGTTGTGGA+GGG 0.654497 1_2:+70577612 None:intergenic
CAAGGTTACCCTCCACAACA+GGG 0.654753 1_2:-70577620 Msa0089150:CDS
CCACCAGGATATCCTCCACA+AGG 0.654889 1_2:-70577638 Msa0089150:CDS
TGTTGTGGAGGATAGCCTTG+AGG 0.658505 1_2:+70577590 None:intergenic
TGTGGAGGATAGCCTTGAGG+TGG 0.659468 1_2:+70577593 None:intergenic
GCGTACTGAGGAGCATATTG+CGG 0.667195 1_2:+70577557 None:intergenic
CCTCGTCAGCAAAATTCAGG+CGG 0.710460 1_2:-70577524 Msa0089150:CDS
CCTTGTGGAGGATATCCTGG+TGG 0.714352 1_2:+70577638 None:intergenic
TGTGGAGGGTAACCTTGTGG+AGG 0.722140 1_2:+70577626 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTCAATTTTAACTT+GGG + chr1_2:70576324-70576343 None:intergenic 10.0%
!!! TTTTAATTTCAATTTTAACT+TGG + chr1_2:70576325-70576344 None:intergenic 10.0%
!! AAATTGAAATTAAAATTGAA+TGG - chr1_2:70576330-70576349 Msa0089150:intron 10.0%
!!! TTTAATTTCAATTTTAACTT+GGG + chr1_2:70576324-70576343 None:intergenic 10.0%
!!! TTTTAATTTCAATTTTAACT+TGG + chr1_2:70576325-70576344 None:intergenic 10.0%
!! AAATTGAAATTAAAATTGAA+TGG - chr1_2:70576330-70576349 Msa0089150:intron 10.0%
!!! TTTTTCTTTCTTTATTTTTG+TGG - chr1_2:70576510-70576529 Msa0089150:intron 15.0%
!!! TTTTCTTTCTTTATTTTTGT+GGG - chr1_2:70576511-70576530 Msa0089150:intron 15.0%
!!! TTTTAATAGCTTGTTAATTT+AGG + chr1_2:70576614-70576633 None:intergenic 15.0%
!! AACAAGCTATTAAAAATTAA+AGG - chr1_2:70576619-70576638 Msa0089150:intron 15.0%
!! AAAAGAATAAGAAACTAATA+AGG + chr1_2:70577225-70577244 None:intergenic 15.0%
!!! TTATTAGTTTCTTATTCTTT+TGG - chr1_2:70577224-70577243 Msa0089150:intron 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG - chr1_2:70577369-70577388 Msa0089150:intron 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG - chr1_2:70577370-70577389 Msa0089150:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr1_2:70577434-70577453 Msa0089150:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr1_2:70577435-70577454 Msa0089150:intron 15.0%
!! ACAAATGAAATTTCTTATAA+AGG + chr1_2:70577858-70577877 None:intergenic 15.0%
!!! TTTTTCTTTCTTTATTTTTG+TGG - chr1_2:70576510-70576529 Msa0089150:intron 15.0%
!!! TTTTCTTTCTTTATTTTTGT+GGG - chr1_2:70576511-70576530 Msa0089150:intron 15.0%
!!! TTTTAATAGCTTGTTAATTT+AGG + chr1_2:70576614-70576633 None:intergenic 15.0%
!! AACAAGCTATTAAAAATTAA+AGG - chr1_2:70576619-70576638 Msa0089150:intron 15.0%
!! AAAAGAATAAGAAACTAATA+AGG + chr1_2:70577225-70577244 None:intergenic 15.0%
!!! TTATTAGTTTCTTATTCTTT+TGG - chr1_2:70577224-70577243 Msa0089150:intron 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG - chr1_2:70577369-70577388 Msa0089150:intron 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG - chr1_2:70577370-70577389 Msa0089150:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr1_2:70577434-70577453 Msa0089150:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr1_2:70577435-70577454 Msa0089150:intron 15.0%
!! ACAAATGAAATTTCTTATAA+AGG + chr1_2:70577858-70577877 None:intergenic 15.0%
!!! TTTTCAATTAAGTGATTTCA+TGG - chr1_2:70576025-70576044 Msa0089150:three_prime_UTR 20.0%
!! AATTTGCAAACAGAAAATTA+AGG + chr1_2:70576294-70576313 None:intergenic 20.0%
!! AAATGAAATGAAAGTGATTA+TGG - chr1_2:70576359-70576378 Msa0089150:intron 20.0%
!! TAAGATAATAGTATGTAGAT+TGG + chr1_2:70576556-70576575 None:intergenic 20.0%
!! TAAAATATACATAAGTCAAG+TGG - chr1_2:70576752-70576771 Msa0089150:intron 20.0%
!! ATACTACTAGTATACTAAAA+AGG - chr1_2:70576881-70576900 Msa0089150:intron 20.0%
!!! TTGATATTTTTAAACTCTTG+TGG + chr1_2:70576991-70577010 None:intergenic 20.0%
!! TTGACAAACAGAAATTAATA+AGG + chr1_2:70577257-70577276 None:intergenic 20.0%
!! TCAAAAACCTTATTAATTTC+TGG - chr1_2:70577273-70577292 Msa0089150:intron 20.0%
!! AAAAGTGGAATATATTGTAT+TGG - chr1_2:70577393-70577412 Msa0089150:intron 20.0%
!! AAAGTGGAATATATTGTATT+GGG - chr1_2:70577394-70577413 Msa0089150:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+AGG - chr1_2:70577504-70577523 Msa0089150:CDS 20.0%
!!! TTTTCAATTAAGTGATTTCA+TGG - chr1_2:70576025-70576044 Msa0089150:three_prime_UTR 20.0%
!! AATTTGCAAACAGAAAATTA+AGG + chr1_2:70576294-70576313 None:intergenic 20.0%
!! AAATGAAATGAAAGTGATTA+TGG - chr1_2:70576359-70576378 Msa0089150:intron 20.0%
!! TAAGATAATAGTATGTAGAT+TGG + chr1_2:70576556-70576575 None:intergenic 20.0%
!! TAAAATATACATAAGTCAAG+TGG - chr1_2:70576752-70576771 Msa0089150:intron 20.0%
!! ATACTACTAGTATACTAAAA+AGG - chr1_2:70576881-70576900 Msa0089150:intron 20.0%
!!! TTGATATTTTTAAACTCTTG+TGG + chr1_2:70576991-70577010 None:intergenic 20.0%
!! TTGACAAACAGAAATTAATA+AGG + chr1_2:70577257-70577276 None:intergenic 20.0%
!! TCAAAAACCTTATTAATTTC+TGG - chr1_2:70577273-70577292 Msa0089150:intron 20.0%
!! AAAAGTGGAATATATTGTAT+TGG - chr1_2:70577393-70577412 Msa0089150:intron 20.0%
!! AAAGTGGAATATATTGTATT+GGG - chr1_2:70577394-70577413 Msa0089150:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+AGG - chr1_2:70577504-70577523 Msa0089150:CDS 20.0%
! GTGTATAATATATATGCATG+CGG + chr1_2:70575948-70575967 None:intergenic 25.0%
! AAGATGATAAATTAACATGC+AGG + chr1_2:70575983-70576002 None:intergenic 25.0%
! AGTGATTTCATGGAATTAAT+AGG - chr1_2:70576035-70576054 Msa0089150:three_prime_UTR 25.0%
!!! AGTAGGTTGGTTTTAATTTT+AGG + chr1_2:70576660-70576679 None:intergenic 25.0%
! AAAGAACAAAGATAAACGTA+TGG + chr1_2:70577086-70577105 None:intergenic 25.0%
!! TTGCATTAATATGCATTTTG+AGG + chr1_2:70577115-70577134 None:intergenic 25.0%
! CAGAAAACCAGAAATTAATA+AGG + chr1_2:70577283-70577302 None:intergenic 25.0%
! TGTATAAATAAAGGGAAAAG+TGG - chr1_2:70577378-70577397 Msa0089150:intron 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG - chr1_2:70577481-70577500 Msa0089150:intron 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG - chr1_2:70577482-70577501 Msa0089150:intron 25.0%
! GTGTATAATATATATGCATG+CGG + chr1_2:70575948-70575967 None:intergenic 25.0%
! AAGATGATAAATTAACATGC+AGG + chr1_2:70575983-70576002 None:intergenic 25.0%
! AGTGATTTCATGGAATTAAT+AGG - chr1_2:70576035-70576054 Msa0089150:three_prime_UTR 25.0%
!!! AGTAGGTTGGTTTTAATTTT+AGG + chr1_2:70576660-70576679 None:intergenic 25.0%
! AAAGAACAAAGATAAACGTA+TGG + chr1_2:70577086-70577105 None:intergenic 25.0%
!! TTGCATTAATATGCATTTTG+AGG + chr1_2:70577115-70577134 None:intergenic 25.0%
! CAGAAAACCAGAAATTAATA+AGG + chr1_2:70577283-70577302 None:intergenic 25.0%
! TGTATAAATAAAGGGAAAAG+TGG - chr1_2:70577378-70577397 Msa0089150:intron 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG - chr1_2:70577481-70577500 Msa0089150:intron 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG - chr1_2:70577482-70577501 Msa0089150:intron 25.0%
TGAAATGAAAGTGATTATGG+AGG - chr1_2:70576362-70576381 Msa0089150:intron 30.0%
ATAACGTGACGAAAGATAAT+AGG + chr1_2:70576469-70576488 None:intergenic 30.0%
ATTGGTATATCCGATGATAT+GGG + chr1_2:70576538-70576557 None:intergenic 30.0%
TATAGTTGTTGAGTACTAGT+AGG + chr1_2:70576677-70576696 None:intergenic 30.0%
ATATACATAAGTCAAGTGGA+AGG - chr1_2:70576756-70576775 Msa0089150:intron 30.0%
TATACATAAGTCAAGTGGAA+GGG - chr1_2:70576757-70576776 Msa0089150:intron 30.0%
! GACAATATACCATTTCTAGA+TGG + chr1_2:70576833-70576852 None:intergenic 30.0%
! TGGTATATTGTCTTGTTCAA+CGG - chr1_2:70576841-70576860 Msa0089150:intron 30.0%
CTAGTATACTAAAAAGGTGA+AGG - chr1_2:70576887-70576906 Msa0089150:intron 30.0%
! CGTTTATCTTTGTTCTTTTG+TGG - chr1_2:70577088-70577107 Msa0089150:intron 30.0%
AATGCAAATGCAACAATTCT+TGG - chr1_2:70577128-70577147 Msa0089150:intron 30.0%
GTGTTTGTATAACTGGAAAT+AGG + chr1_2:70577192-70577211 None:intergenic 30.0%
TAAAGTAGGTCAATTAAACC+GGG + chr1_2:70577334-70577353 None:intergenic 30.0%
CTAAAGTAGGTCAATTAAAC+CGG + chr1_2:70577335-70577354 None:intergenic 30.0%
TCTGTGTGAAAAACTAAAGT+AGG + chr1_2:70577348-70577367 None:intergenic 30.0%
AGAAAATAGGTCAGTTATTC+CGG - chr1_2:70577517-70577536 Msa0089150:CDS 30.0%
AATTTGTAAATGTTGCAGCT+TGG - chr1_2:70577591-70577610 Msa0089150:CDS 30.0%
TGAAATGAAAGTGATTATGG+AGG - chr1_2:70576362-70576381 Msa0089150:intron 30.0%
ATAACGTGACGAAAGATAAT+AGG + chr1_2:70576469-70576488 None:intergenic 30.0%
ATTGGTATATCCGATGATAT+GGG + chr1_2:70576538-70576557 None:intergenic 30.0%
TATAGTTGTTGAGTACTAGT+AGG + chr1_2:70576677-70576696 None:intergenic 30.0%
ATATACATAAGTCAAGTGGA+AGG - chr1_2:70576756-70576775 Msa0089150:intron 30.0%
TATACATAAGTCAAGTGGAA+GGG - chr1_2:70576757-70576776 Msa0089150:intron 30.0%
! GACAATATACCATTTCTAGA+TGG + chr1_2:70576833-70576852 None:intergenic 30.0%
! TGGTATATTGTCTTGTTCAA+CGG - chr1_2:70576841-70576860 Msa0089150:intron 30.0%
CTAGTATACTAAAAAGGTGA+AGG - chr1_2:70576887-70576906 Msa0089150:intron 30.0%
! CGTTTATCTTTGTTCTTTTG+TGG - chr1_2:70577088-70577107 Msa0089150:intron 30.0%
AATGCAAATGCAACAATTCT+TGG - chr1_2:70577128-70577147 Msa0089150:intron 30.0%
GTGTTTGTATAACTGGAAAT+AGG + chr1_2:70577192-70577211 None:intergenic 30.0%
TAAAGTAGGTCAATTAAACC+GGG + chr1_2:70577334-70577353 None:intergenic 30.0%
CTAAAGTAGGTCAATTAAAC+CGG + chr1_2:70577335-70577354 None:intergenic 30.0%
TCTGTGTGAAAAACTAAAGT+AGG + chr1_2:70577348-70577367 None:intergenic 30.0%
AGAAAATAGGTCAGTTATTC+CGG - chr1_2:70577517-70577536 Msa0089150:CDS 30.0%
AATTTGTAAATGTTGCAGCT+TGG - chr1_2:70577591-70577610 Msa0089150:CDS 30.0%
AGAAGAGAAAGTTGGAAATG+GGG + chr1_2:70575830-70575849 None:intergenic 35.0%
GAGAAGAGAAAGTTGGAAAT+GGG + chr1_2:70575831-70575850 None:intergenic 35.0%
AGAGAAGAGAAAGTTGGAAA+TGG + chr1_2:70575832-70575851 None:intergenic 35.0%
TGGTTGTAGTAACTCATTGT+GGG + chr1_2:70575871-70575890 None:intergenic 35.0%
TTGGTTGTAGTAACTCATTG+TGG + chr1_2:70575872-70575891 None:intergenic 35.0%
CATGGAATTAATAGGTTACC+CGG - chr1_2:70576043-70576062 Msa0089150:three_prime_UTR 35.0%
TAAGGAACAATCCAGAAAGA+TGG + chr1_2:70576276-70576295 None:intergenic 35.0%
GGATATTCATTCAGATCAAG+CGG - chr1_2:70576383-70576402 Msa0089150:intron 35.0%
GATTGGTATATCCGATGATA+TGG + chr1_2:70576539-70576558 None:intergenic 35.0%
ATACATAAGTCAAGTGGAAG+GGG - chr1_2:70576758-70576777 Msa0089150:intron 35.0%
! TAAAAAGGTGAAGGTGTATG+TGG - chr1_2:70576896-70576915 Msa0089150:intron 35.0%
TACGTGTGTGTTTGTATAAC+TGG + chr1_2:70577199-70577218 None:intergenic 35.0%
GAAAATAGGTCAGTTATTCC+GGG - chr1_2:70577518-70577537 Msa0089150:CDS 35.0%
CAGATCAGATTACATCACAT+TGG + chr1_2:70577564-70577583 None:intergenic 35.0%
AGACATTACTTTGCACAGAT+TGG - chr1_2:70577657-70577676 Msa0089150:CDS 35.0%
AAGGTAACAAGCATCACAAA+AGG + chr1_2:70577839-70577858 None:intergenic 35.0%
AGAAGAGAAAGTTGGAAATG+GGG + chr1_2:70575830-70575849 None:intergenic 35.0%
GAGAAGAGAAAGTTGGAAAT+GGG + chr1_2:70575831-70575850 None:intergenic 35.0%
AGAGAAGAGAAAGTTGGAAA+TGG + chr1_2:70575832-70575851 None:intergenic 35.0%
TGGTTGTAGTAACTCATTGT+GGG + chr1_2:70575871-70575890 None:intergenic 35.0%
TTGGTTGTAGTAACTCATTG+TGG + chr1_2:70575872-70575891 None:intergenic 35.0%
CATGGAATTAATAGGTTACC+CGG - chr1_2:70576043-70576062 Msa0089150:three_prime_UTR 35.0%
TAAGGAACAATCCAGAAAGA+TGG + chr1_2:70576276-70576295 None:intergenic 35.0%
GGATATTCATTCAGATCAAG+CGG - chr1_2:70576383-70576402 Msa0089150:intron 35.0%
GATTGGTATATCCGATGATA+TGG + chr1_2:70576539-70576558 None:intergenic 35.0%
ATACATAAGTCAAGTGGAAG+GGG - chr1_2:70576758-70576777 Msa0089150:intron 35.0%
! TAAAAAGGTGAAGGTGTATG+TGG - chr1_2:70576896-70576915 Msa0089150:intron 35.0%
TACGTGTGTGTTTGTATAAC+TGG + chr1_2:70577199-70577218 None:intergenic 35.0%
GAAAATAGGTCAGTTATTCC+GGG - chr1_2:70577518-70577537 Msa0089150:CDS 35.0%
CAGATCAGATTACATCACAT+TGG + chr1_2:70577564-70577583 None:intergenic 35.0%
AGACATTACTTTGCACAGAT+TGG - chr1_2:70577657-70577676 Msa0089150:CDS 35.0%
AAGGTAACAAGCATCACAAA+AGG + chr1_2:70577839-70577858 None:intergenic 35.0%
TAGTGGAGAGAAGAGAAAGT+TGG + chr1_2:70575838-70575857 None:intergenic 40.0%
TTGTAGTAACTCATTGTGGG+GGG + chr1_2:70575868-70575887 None:intergenic 40.0%
GTTGTAGTAACTCATTGTGG+GGG + chr1_2:70575869-70575888 None:intergenic 40.0%
GGTTGTAGTAACTCATTGTG+GGG + chr1_2:70575870-70575889 None:intergenic 40.0%
ATGCGGTTAAGAATAGAGGA+TGG + chr1_2:70575931-70575950 None:intergenic 40.0%
ATGCATGCGGTTAAGAATAG+AGG + chr1_2:70575935-70575954 None:intergenic 40.0%
! GCATCTTTTCCATAAGTCTC+CGG + chr1_2:70576071-70576090 None:intergenic 40.0%
! TTTTGTGGGACCCATATCAT+CGG - chr1_2:70576525-70576544 Msa0089150:intron 40.0%
GTTGTTGAGTACTAGTAGGT+TGG + chr1_2:70576673-70576692 None:intergenic 40.0%
TAATGATGTCAATGACGTGC+AGG - chr1_2:70576801-70576820 Msa0089150:intron 40.0%
AATGATGTCAATGACGTGCA+GGG - chr1_2:70576802-70576821 Msa0089150:intron 40.0%
AGGGTTTAGCCATCTAGAAA+TGG - chr1_2:70576821-70576840 Msa0089150:intron 40.0%
GAATTAGGCAGTACGAAAGT+TGG + chr1_2:70577794-70577813 None:intergenic 40.0%
TTTCGTACTGCCTAATTCAC+AGG - chr1_2:70577796-70577815 Msa0089150:intron 40.0%
TACTGCCTAATTCACAGGAT+TGG - chr1_2:70577801-70577820 Msa0089150:intron 40.0%
TAGTGGAGAGAAGAGAAAGT+TGG + chr1_2:70575838-70575857 None:intergenic 40.0%
TTGTAGTAACTCATTGTGGG+GGG + chr1_2:70575868-70575887 None:intergenic 40.0%
GTTGTAGTAACTCATTGTGG+GGG + chr1_2:70575869-70575888 None:intergenic 40.0%
GGTTGTAGTAACTCATTGTG+GGG + chr1_2:70575870-70575889 None:intergenic 40.0%
ATGCGGTTAAGAATAGAGGA+TGG + chr1_2:70575931-70575950 None:intergenic 40.0%
ATGCATGCGGTTAAGAATAG+AGG + chr1_2:70575935-70575954 None:intergenic 40.0%
! GCATCTTTTCCATAAGTCTC+CGG + chr1_2:70576071-70576090 None:intergenic 40.0%
! TTTTGTGGGACCCATATCAT+CGG - chr1_2:70576525-70576544 Msa0089150:intron 40.0%
GTTGTTGAGTACTAGTAGGT+TGG + chr1_2:70576673-70576692 None:intergenic 40.0%
TAATGATGTCAATGACGTGC+AGG - chr1_2:70576801-70576820 Msa0089150:intron 40.0%
AATGATGTCAATGACGTGCA+GGG - chr1_2:70576802-70576821 Msa0089150:intron 40.0%
AGGGTTTAGCCATCTAGAAA+TGG - chr1_2:70576821-70576840 Msa0089150:intron 40.0%
GAATTAGGCAGTACGAAAGT+TGG + chr1_2:70577794-70577813 None:intergenic 40.0%
TTTCGTACTGCCTAATTCAC+AGG - chr1_2:70577796-70577815 Msa0089150:intron 40.0%
TACTGCCTAATTCACAGGAT+TGG - chr1_2:70577801-70577820 Msa0089150:intron 40.0%
AATAGAGGATGGACCTTGCT+GGG + chr1_2:70575920-70575939 None:intergenic 45.0%
TAACCTTGTGGAGGATATCC+TGG + chr1_2:70576104-70576123 None:intergenic 45.0%
TACTCCTCTCTCCATCTTTC+TGG - chr1_2:70576262-70576281 Msa0089150:intron 45.0%
CAATCCAGAAAGATGGAGAG+AGG + chr1_2:70576269-70576288 None:intergenic 45.0%
ATCTTGGAATTGATCGCTGG+TGG - chr1_2:70576410-70576429 Msa0089150:intron 45.0%
ATAAGTCAAGTGGAAGGGGT+GGG - chr1_2:70576762-70576781 Msa0089150:intron 45.0%
GTAGGTCAATTAAACCGGGA+CGG + chr1_2:70577330-70577349 None:intergenic 45.0%
ATAGGTCAGTTATTCCGGGA+CGG - chr1_2:70577522-70577541 Msa0089150:CDS 45.0%
! GCCTAATTCACAGGATTGGT+CGG - chr1_2:70577805-70577824 Msa0089150:intron 45.0%
ACCGACCAATCCTGTGAATT+AGG + chr1_2:70577809-70577828 None:intergenic 45.0%
AATAGAGGATGGACCTTGCT+GGG + chr1_2:70575920-70575939 None:intergenic 45.0%
TAACCTTGTGGAGGATATCC+TGG + chr1_2:70576104-70576123 None:intergenic 45.0%
TACTCCTCTCTCCATCTTTC+TGG - chr1_2:70576262-70576281 Msa0089150:intron 45.0%
CAATCCAGAAAGATGGAGAG+AGG + chr1_2:70576269-70576288 None:intergenic 45.0%
ATCTTGGAATTGATCGCTGG+TGG - chr1_2:70576410-70576429 Msa0089150:intron 45.0%
ATAAGTCAAGTGGAAGGGGT+GGG - chr1_2:70576762-70576781 Msa0089150:intron 45.0%
GTAGGTCAATTAAACCGGGA+CGG + chr1_2:70577330-70577349 None:intergenic 45.0%
ATAGGTCAGTTATTCCGGGA+CGG - chr1_2:70577522-70577541 Msa0089150:CDS 45.0%
! GCCTAATTCACAGGATTGGT+CGG - chr1_2:70577805-70577824 Msa0089150:intron 45.0%
ACCGACCAATCCTGTGAATT+AGG + chr1_2:70577809-70577828 None:intergenic 45.0%
!! TCTAATAATAAAATTAAAAA+AGG + chr1_2:70576943-70576962 None:intergenic 5.0%
!! TCTAATAATAAAATTAAAAA+AGG + chr1_2:70576943-70576962 None:intergenic 5.0%
TAACTCATTGTGGGGGGTAG+GGG + chr1_2:70575862-70575881 None:intergenic 50.0%
GTAACTCATTGTGGGGGGTA+GGG + chr1_2:70575863-70575882 None:intergenic 50.0%
AGTAACTCATTGTGGGGGGT+AGG + chr1_2:70575864-70575883 None:intergenic 50.0%
ATAGAGGATGGACCTTGCTG+GGG + chr1_2:70575919-70575938 None:intergenic 50.0%
GAATAGAGGATGGACCTTGC+TGG + chr1_2:70575921-70575940 None:intergenic 50.0%
ATTAATAGGTTACCCGGCGC+CGG - chr1_2:70576049-70576068 Msa0089150:three_prime_UTR 50.0%
AAAGATGCTTACCCTCCACC+AGG - chr1_2:70576083-70576102 Msa0089150:three_prime_UTR 50.0%
TGTTGTGGAGGGTAACCTTG+TGG + chr1_2:70576116-70576135 None:intergenic 50.0%
ACAAGGTTACCCTCCACAAC+AGG - chr1_2:70576115-70576134 Msa0089150:CDS 50.0%
CAAGGTTACCCTCCACAACA+GGG - chr1_2:70576116-70576135 Msa0089150:CDS 50.0%
! TGTTGTGGAGGATAGCCTTG+AGG + chr1_2:70576149-70576168 None:intergenic 50.0%
CAAGGCTATCCTCCACAACA+AGG - chr1_2:70576149-70576168 Msa0089150:intron 50.0%
! ACTGAGGAGCATATTGCGGT+GGG + chr1_2:70576178-70576197 None:intergenic 50.0%
! TACTGAGGAGCATATTGCGG+TGG + chr1_2:70576179-70576198 None:intergenic 50.0%
! GCGTACTGAGGAGCATATTG+CGG + chr1_2:70576182-70576201 None:intergenic 50.0%
! GAATTTTGCTGACGAGGTGG+TGG + chr1_2:70576209-70576228 None:intergenic 50.0%
! CCTGAATTTTGCTGACGAGG+TGG + chr1_2:70576212-70576231 None:intergenic 50.0%
CCACCTCGTCAGCAAAATTC+AGG - chr1_2:70576209-70576228 Msa0089150:intron 50.0%
CCTCGTCAGCAAAATTCAGG+CGG - chr1_2:70576212-70576231 Msa0089150:intron 50.0%
GCGATCTTGGAATTGATCGC+TGG - chr1_2:70576407-70576426 Msa0089150:intron 50.0%
CATAAGTCAAGTGGAAGGGG+TGG - chr1_2:70576761-70576780 Msa0089150:intron 50.0%
GTCAATTAAACCGGGACGGA+GGG + chr1_2:70577326-70577345 None:intergenic 50.0%
TCTCTGCTGTTGCTGTCTCT+TGG - chr1_2:70577618-70577637 Msa0089150:CDS 50.0%
! CTCTTGGATGCGTGCTTTTG+AGG - chr1_2:70577634-70577653 Msa0089150:CDS 50.0%
TAACTCATTGTGGGGGGTAG+GGG + chr1_2:70575862-70575881 None:intergenic 50.0%
GTAACTCATTGTGGGGGGTA+GGG + chr1_2:70575863-70575882 None:intergenic 50.0%
AGTAACTCATTGTGGGGGGT+AGG + chr1_2:70575864-70575883 None:intergenic 50.0%
ATAGAGGATGGACCTTGCTG+GGG + chr1_2:70575919-70575938 None:intergenic 50.0%
GAATAGAGGATGGACCTTGC+TGG + chr1_2:70575921-70575940 None:intergenic 50.0%
ATTAATAGGTTACCCGGCGC+CGG - chr1_2:70576049-70576068 Msa0089150:three_prime_UTR 50.0%
AAAGATGCTTACCCTCCACC+AGG - chr1_2:70576083-70576102 Msa0089150:three_prime_UTR 50.0%
TGTTGTGGAGGGTAACCTTG+TGG + chr1_2:70576116-70576135 None:intergenic 50.0%
ACAAGGTTACCCTCCACAAC+AGG - chr1_2:70576115-70576134 Msa0089150:CDS 50.0%
CAAGGTTACCCTCCACAACA+GGG - chr1_2:70576116-70576135 Msa0089150:CDS 50.0%
! TGTTGTGGAGGATAGCCTTG+AGG + chr1_2:70576149-70576168 None:intergenic 50.0%
CAAGGCTATCCTCCACAACA+AGG - chr1_2:70576149-70576168 Msa0089150:intron 50.0%
! ACTGAGGAGCATATTGCGGT+GGG + chr1_2:70576178-70576197 None:intergenic 50.0%
! TACTGAGGAGCATATTGCGG+TGG + chr1_2:70576179-70576198 None:intergenic 50.0%
! GCGTACTGAGGAGCATATTG+CGG + chr1_2:70576182-70576201 None:intergenic 50.0%
! GAATTTTGCTGACGAGGTGG+TGG + chr1_2:70576209-70576228 None:intergenic 50.0%
! CCTGAATTTTGCTGACGAGG+TGG + chr1_2:70576212-70576231 None:intergenic 50.0%
CCACCTCGTCAGCAAAATTC+AGG - chr1_2:70576209-70576228 Msa0089150:intron 50.0%
CCTCGTCAGCAAAATTCAGG+CGG - chr1_2:70576212-70576231 Msa0089150:intron 50.0%
GCGATCTTGGAATTGATCGC+TGG - chr1_2:70576407-70576426 Msa0089150:intron 50.0%
CATAAGTCAAGTGGAAGGGG+TGG - chr1_2:70576761-70576780 Msa0089150:intron 50.0%
GTCAATTAAACCGGGACGGA+GGG + chr1_2:70577326-70577345 None:intergenic 50.0%
TCTCTGCTGTTGCTGTCTCT+TGG - chr1_2:70577618-70577637 Msa0089150:CDS 50.0%
! CTCTTGGATGCGTGCTTTTG+AGG - chr1_2:70577634-70577653 Msa0089150:CDS 50.0%
AACTCATTGTGGGGGGTAGG+GGG + chr1_2:70575861-70575880 None:intergenic 55.0%
TTTCCATAAGTCTCCGGCGC+CGG + chr1_2:70576065-70576084 None:intergenic 55.0%
GTGGAGGATATCCTGGTGGA+GGG + chr1_2:70576097-70576116 None:intergenic 55.0%
TGTGGAGGATATCCTGGTGG+AGG + chr1_2:70576098-70576117 None:intergenic 55.0%
CCTTGTGGAGGATATCCTGG+TGG + chr1_2:70576101-70576120 None:intergenic 55.0%
CCACCAGGATATCCTCCACA+AGG - chr1_2:70576098-70576117 Msa0089150:exon 55.0%
TGTGGAGGGTAACCTTGTGG+AGG + chr1_2:70576113-70576132 None:intergenic 55.0%
GTGGATAGCCCTGTTGTGGA+GGG + chr1_2:70576127-70576146 None:intergenic 55.0%
TGAGGTGGATAGCCCTGTTG+TGG + chr1_2:70576131-70576150 None:intergenic 55.0%
CAACAGGGCTATCCACCTCA+AGG - chr1_2:70576131-70576150 Msa0089150:intron 55.0%
! TGTGGAGGATAGCCTTGAGG+TGG + chr1_2:70576146-70576165 None:intergenic 55.0%
! CCGCCTGAATTTTGCTGACG+AGG + chr1_2:70576215-70576234 None:intergenic 55.0%
CAGCAAAATTCAGGCGGTGC+TGG - chr1_2:70576218-70576237 Msa0089150:intron 55.0%
CAGATCAAGCGGTGCGATCT+TGG - chr1_2:70576394-70576413 Msa0089150:intron 55.0%
GTGTTATACTCCCTCCGTCC+CGG - chr1_2:70577313-70577332 Msa0089150:intron 55.0%
GGTCAATTAAACCGGGACGG+AGG + chr1_2:70577327-70577346 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG - chr1_2:70577526-70577545 Msa0089150:CDS 55.0%
AGCATATACTCCCTCCGTCC+CGG + chr1_2:70577539-70577558 None:intergenic 55.0%
AACTCATTGTGGGGGGTAGG+GGG + chr1_2:70575861-70575880 None:intergenic 55.0%
TTTCCATAAGTCTCCGGCGC+CGG + chr1_2:70576065-70576084 None:intergenic 55.0%
GTGGAGGATATCCTGGTGGA+GGG + chr1_2:70576097-70576116 None:intergenic 55.0%
TGTGGAGGATATCCTGGTGG+AGG + chr1_2:70576098-70576117 None:intergenic 55.0%
CCTTGTGGAGGATATCCTGG+TGG + chr1_2:70576101-70576120 None:intergenic 55.0%
CCACCAGGATATCCTCCACA+AGG - chr1_2:70576098-70576117 Msa0089150:exon 55.0%
TGTGGAGGGTAACCTTGTGG+AGG + chr1_2:70576113-70576132 None:intergenic 55.0%
GTGGATAGCCCTGTTGTGGA+GGG + chr1_2:70576127-70576146 None:intergenic 55.0%
TGAGGTGGATAGCCCTGTTG+TGG + chr1_2:70576131-70576150 None:intergenic 55.0%
CAACAGGGCTATCCACCTCA+AGG - chr1_2:70576131-70576150 Msa0089150:intron 55.0%
! TGTGGAGGATAGCCTTGAGG+TGG + chr1_2:70576146-70576165 None:intergenic 55.0%
! CCGCCTGAATTTTGCTGACG+AGG + chr1_2:70576215-70576234 None:intergenic 55.0%
CAGCAAAATTCAGGCGGTGC+TGG - chr1_2:70576218-70576237 Msa0089150:intron 55.0%
CAGATCAAGCGGTGCGATCT+TGG - chr1_2:70576394-70576413 Msa0089150:intron 55.0%
GTGTTATACTCCCTCCGTCC+CGG - chr1_2:70577313-70577332 Msa0089150:intron 55.0%
GGTCAATTAAACCGGGACGG+AGG + chr1_2:70577327-70577346 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG - chr1_2:70577526-70577545 Msa0089150:CDS 55.0%
AGCATATACTCCCTCCGTCC+CGG + chr1_2:70577539-70577558 None:intergenic 55.0%
AACCAACAGCAACCGCCCGT+CGG - chr1_2:70575886-70575905 Msa0089150:three_prime_UTR 60.0%
TTCCATAAGTCTCCGGCGCC+GGG + chr1_2:70576064-70576083 None:intergenic 60.0%
GGTGGATAGCCCTGTTGTGG+AGG + chr1_2:70576128-70576147 None:intergenic 60.0%
!! GGTGGGTAGCCTTGTTGTGG+AGG + chr1_2:70576161-70576180 None:intergenic 60.0%
!! TGCGGTGGGTAGCCTTGTTG+TGG + chr1_2:70576164-70576183 None:intergenic 60.0%
!! TTCAGGCGGTGCTGGTTGCA+TGG - chr1_2:70576226-70576245 Msa0089150:intron 60.0%
!! GTGCTGGTTGCATGGAAGGC+TGG - chr1_2:70576234-70576253 Msa0089150:intron 60.0%
GGTCAGTTATTCCGGGACGG+AGG - chr1_2:70577525-70577544 Msa0089150:CDS 60.0%
AACCAACAGCAACCGCCCGT+CGG - chr1_2:70575886-70575905 Msa0089150:three_prime_UTR 60.0%
TTCCATAAGTCTCCGGCGCC+GGG + chr1_2:70576064-70576083 None:intergenic 60.0%
GGTGGATAGCCCTGTTGTGG+AGG + chr1_2:70576128-70576147 None:intergenic 60.0%
!! GGTGGGTAGCCTTGTTGTGG+AGG + chr1_2:70576161-70576180 None:intergenic 60.0%
!! TGCGGTGGGTAGCCTTGTTG+TGG + chr1_2:70576164-70576183 None:intergenic 60.0%
!! TTCAGGCGGTGCTGGTTGCA+TGG - chr1_2:70576226-70576245 Msa0089150:intron 60.0%
!! GTGCTGGTTGCATGGAAGGC+TGG - chr1_2:70576234-70576253 Msa0089150:intron 60.0%
GGTCAGTTATTCCGGGACGG+AGG - chr1_2:70577525-70577544 Msa0089150:CDS 60.0%
TTGTGGGGGGTAGGGGGTAG+TGG + chr1_2:70575855-70575874 None:intergenic 65.0%
TTGCTGGGGAGGAACGCCGA+CGG + chr1_2:70575905-70575924 None:intergenic 65.0%
GAGGATGGACCTTGCTGGGG+AGG + chr1_2:70575916-70575935 None:intergenic 65.0%
! TACCCGGCGCCGGAGACTTA+TGG - chr1_2:70576059-70576078 Msa0089150:three_prime_UTR 65.0%
! GGTGGTGGCTGAGCGTACTG+AGG + chr1_2:70576194-70576213 None:intergenic 65.0%
!! GGCGGTGCTGGTTGCATGGA+AGG - chr1_2:70576230-70576249 Msa0089150:intron 65.0%
TTGTGGGGGGTAGGGGGTAG+TGG + chr1_2:70575855-70575874 None:intergenic 65.0%
TTGCTGGGGAGGAACGCCGA+CGG + chr1_2:70575905-70575924 None:intergenic 65.0%
GAGGATGGACCTTGCTGGGG+AGG + chr1_2:70575916-70575935 None:intergenic 65.0%
! TACCCGGCGCCGGAGACTTA+TGG - chr1_2:70576059-70576078 Msa0089150:three_prime_UTR 65.0%
! GGTGGTGGCTGAGCGTACTG+AGG + chr1_2:70576194-70576213 None:intergenic 65.0%
!! GGCGGTGCTGGTTGCATGGA+AGG - chr1_2:70576230-70576249 Msa0089150:intron 65.0%
TGCTGGGGAGGAACGCCGAC+GGG + chr1_2:70575904-70575923 None:intergenic 70.0%
GTCGGCGTTCCTCCCCAGCA+AGG - chr1_2:70575904-70575923 Msa0089150:three_prime_UTR 70.0%
TGCTGGGGAGGAACGCCGAC+GGG + chr1_2:70575904-70575923 None:intergenic 70.0%
GTCGGCGTTCCTCCCCAGCA+AGG - chr1_2:70575904-70575923 Msa0089150:three_prime_UTR 70.0%
CGCCGACGGGCGGTTGCTGT+TGG + chr1_2:70575891-70575910 None:intergenic 75.0%
TGGGGAGGAACGCCGACGGG+CGG + chr1_2:70575901-70575920 None:intergenic 75.0%
CGCCGACGGGCGGTTGCTGT+TGG + chr1_2:70575891-70575910 None:intergenic 75.0%
TGGGGAGGAACGCCGACGGG+CGG + chr1_2:70575901-70575920 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 70575797 70577961 70575797 ID=Msa0089150;Name=Msa0089150
chr1_2 mRNA 70575797 70577961 70575797 ID=Msa0089150-mRNA-1;Parent=Msa0089150;Name=Msa0089150-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77|1|1|1|0.5|0.33|3|307|98
chr1_2 exon 70575797 70576149 70575797 ID=Msa0089150-mRNA-1:exon:24269;Parent=Msa0089150-mRNA-1
chr1_2 exon 70577503 70577701 70577503 ID=Msa0089150-mRNA-1:exon:24268;Parent=Msa0089150-mRNA-1
chr1_2 exon 70577833 70577961 70577833 ID=Msa0089150-mRNA-1:exon:24267;Parent=Msa0089150-mRNA-1
chr1_2 five_prime_UTR 70577885 70577961 70577885 ID=Msa0089150-mRNA-1:five_prime_utr;Parent=Msa0089150-mRNA-1
chr1_2 CDS 70577833 70577884 70577833 ID=Msa0089150-mRNA-1:cds;Parent=Msa0089150-mRNA-1
chr1_2 CDS 70577503 70577701 70577503 ID=Msa0089150-mRNA-1:cds;Parent=Msa0089150-mRNA-1
chr1_2 CDS 70576104 70576149 70576104 ID=Msa0089150-mRNA-1:cds;Parent=Msa0089150-mRNA-1
chr1_2 three_prime_UTR 70575797 70576103 70575797 ID=Msa0089150-mRNA-1:three_prime_utr;Parent=Msa0089150-mRNA-1
Gene Sequence

>Msa0089150

ATGAGTTACTACAACCAACAGCAACCGCCCGTCGGCGTTCCTCCCCAGCAAGGTTACCCGGCGCCGGAGACTTATGGAAAAGATGCTTACCCTCCACCAGGATATCCTCCACAAGGTTACCCTCCACAACAGGGCTATCCACCTCAAGGCTATCCTCCACAACAAGGCTACCCACCGCAATATGCTCCTCAGTACGCTCAGCCACCACCTCGTCAGCAAAATTCAGGCGGTGCTGGTTGCATGGAAGGCTGCTTGGCTGCTCTCTGCTGTTGCTGTCTCTTGGATGCGTGCTTTTGA
Protein sequence

>Msa0089150

MSYYNQQQPPVGVPPQQGYPAPETYGKDAYPPPGYPPQGYPPQQGYPPQGYPPQQGYPPQYAPQYAQPPPRQQNSGGAGCMEGCLAALCCCCLLDACF*