AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0103170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0103170 MtrunA17_Chr1g0146561 98.276 232 4 0 1 232 1 232 1.39e-169 466
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0103170 AT4G31460.1 66.497 197 66 0 1 197 1 197 2.53e-96 280

Find 70 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGAATAAACTCTTATTT+TGG 0.198796 1_3:+10271011 None:intergenic
ACCCATAACAACTTTGCTTT+TGG 0.225318 1_3:+10273743 None:intergenic
GATGAAGAAGGTTCTGAAAA+AGG 0.273471 1_3:-10273820 Msa0103170:CDS
GAAAGTTTAGAGCATAAAAC+TGG 0.299754 1_3:+10271060 None:intergenic
TCCCAAAAGCAAAGTTGTTA+TGG 0.322622 1_3:-10273745 Msa0103170:CDS
TTTGTATGAGGCAATTGTTT+TGG 0.323942 1_3:-10270820 Msa0103170:three_prime_UTR
CAAAAGATGGACACAGAAAT+AGG 0.324056 1_3:-10271495 Msa0103170:CDS
TTGTGAAGTGATAGGGAAAA+TGG 0.333186 1_3:-10273862 Msa0103170:intron
TGGACCTTCTGGCTTGTATA+AGG 0.347875 1_3:-10270800 Msa0103170:three_prime_UTR
GCTGGGAAACATATCCAGTT+TGG 0.350098 1_3:-10273699 Msa0103170:CDS
CCCAAAAGCAAAGTTGTTAT+GGG 0.364570 1_3:-10273744 Msa0103170:CDS
CTGGGAAACATATCCAGTTT+GGG 0.371312 1_3:-10273698 Msa0103170:CDS
TCAAACTTCGCTTCATCCTC+CGG 0.377365 1_3:+10271402 None:intergenic
TTAGTGAAGATGGTGGTAAC+AGG 0.399831 1_3:-10273668 Msa0103170:intron
CACTAACACTATTCCCAAAC+TGG 0.402543 1_3:+10273685 None:intergenic
ACAGTGATGAAAGGTTAAAA+TGG 0.407362 1_3:+10274029 None:intergenic
GGATGAAGCGAAGTTTGAAC+AGG 0.428127 1_3:-10271397 Msa0103170:CDS
AGCCTCTTATCTTGTACGTT+AGG 0.429435 1_3:+10271627 None:intergenic
TCTCCAACTTTGAGCCTGTC+AGG 0.432513 1_3:+10271183 None:intergenic
GGGAATAGTGTTAGTGAAGA+TGG 0.437529 1_3:-10273678 Msa0103170:CDS
CAAACTTCGCTTCATCCTCC+GGG 0.443570 1_3:+10271403 None:intergenic
AGATTTGAGATTAGCTGAAA+GGG 0.447839 1_3:-10271367 Msa0103170:CDS
GAGAAGTTGTATGAAGAGCT+TGG 0.456277 1_3:-10271450 Msa0103170:CDS
GCACTCTTAGTAAATCTAGA+AGG 0.457321 1_3:-10270966 Msa0103170:three_prime_UTR
AACCTAACGTACAAGATAAG+AGG 0.473606 1_3:-10271629 Msa0103170:CDS
CGAAGGAATCGATAGAGAAA+TGG 0.474673 1_3:-10273770 Msa0103170:CDS
ATAGGGAAAATGGCGTTTAG+AGG 0.475804 1_3:-10273852 Msa0103170:exon
AAGATTTGAGATTAGCTGAA+AGG 0.477923 1_3:-10271368 Msa0103170:CDS
TAAGAGTTTATTCCTTGTAG+TGG 0.480038 1_3:-10271005 Msa0103170:three_prime_UTR
TAATACGAATGTGACGATCT+AGG 0.481354 1_3:+10271592 None:intergenic
GAAGACTCCTTATCAAAAGA+TGG 0.484789 1_3:-10271508 Msa0103170:CDS
TAGTTTGTGCTTGTTCATGC+TGG 0.485930 1_3:+10271311 None:intergenic
TTTAACCTTTCATCACTGTC+CGG 0.491706 1_3:-10274025 Msa0103170:five_prime_UTR
ATTGCCTCATACAAATGAAA+AGG 0.495615 1_3:+10270828 None:intergenic
AGGAAAAGAGATGATGAAGA+AGG 0.509718 1_3:-10273832 Msa0103170:CDS
CTGATAGGACAAGGAGAACA+TGG 0.516494 1_3:-10271653 Msa0103170:intron
CGAGGGAGCTGCTAAAATTG+AGG 0.519910 1_3:-10271262 Msa0103170:CDS
CTTCGTTGCATCGACAAGGC+AGG 0.525155 1_3:-10271552 Msa0103170:CDS
GAGGGAGCTGCTAAAATTGA+GGG 0.526843 1_3:-10271261 Msa0103170:CDS
GTATTTGGGTGCACCTATGC+AGG 0.529294 1_3:-10270904 Msa0103170:three_prime_UTR
GAACAAGCACAAACTAATTG+AGG 0.531079 1_3:-10271304 Msa0103170:CDS
AAGAGTTTATTCCTTGTAGT+GGG 0.532552 1_3:-10271004 Msa0103170:three_prime_UTR
ATTGAGGTAGAAAGTAAAGA+TGG 0.533016 1_3:-10271288 Msa0103170:CDS
CTCATCGAGTATGAAAGTCT+TGG 0.534565 1_3:-10271104 Msa0103170:three_prime_UTR
ACTCGATTCATCAAAGCGAT+TGG 0.536622 1_3:-10271229 Msa0103170:CDS
TGAAGAGCTTGGAAAGAAAG+AGG 0.537302 1_3:-10271439 Msa0103170:CDS
GCTCCTGACAGGCTCAAAGT+TGG 0.542014 1_3:-10271186 Msa0103170:CDS
GGAAAATTGATCCCACTACA+AGG 0.546835 1_3:+10270993 None:intergenic
CGTATGTGTTAGCTCCTGAC+AGG 0.548037 1_3:-10271197 Msa0103170:CDS
CGCCCTTCGTTGCATCGACA+AGG 0.548075 1_3:-10271556 Msa0103170:CDS
GGAGCTAACACATACGAGAC+AGG 0.555598 1_3:+10271204 None:intergenic
TGCCTTGTCGATGCAACGAA+GGG 0.567208 1_3:+10271554 None:intergenic
CTGCCTTGTCGATGCAACGA+AGG 0.571094 1_3:+10271553 None:intergenic
AAACACTGATAAACTCGTCC+AGG 0.571966 1_3:-10274000 Msa0103170:intron
TCTATCGATTCCTTCGCTCG+TGG 0.572095 1_3:+10273777 None:intergenic
GAGATATTAGTCACCTGCAT+AGG 0.584310 1_3:+10270891 None:intergenic
AGATTTACTAAGAGTGCATG+AGG 0.592242 1_3:+10270972 None:intergenic
AGAAAGTAAAGATGGTGACG+AGG 0.602281 1_3:-10271280 Msa0103170:CDS
ATGAAAGGTTAAAATGGCAA+CGG 0.620217 1_3:+10274035 None:intergenic
GAATATGACTCCACGAGCGA+AGG 0.626713 1_3:-10273787 Msa0103170:CDS
GATGCAACGAAGGGCGTGAG+TGG 0.648741 1_3:+10271563 None:intergenic
CGTTGCATCGACAAGGCAGG+CGG 0.656785 1_3:-10271549 Msa0103170:CDS
GTTATGGGTCGTGCTAAACG+TGG 0.661514 1_3:-10273729 Msa0103170:CDS
GATGTTATGTCAGCTGATGA+CGG 0.663369 1_3:-10271146 Msa0103170:three_prime_UTR
AGTACCTTATACAAGCCAGA+AGG 0.672103 1_3:+10270796 None:intergenic
AAACTTCGCTTCATCCTCCG+GGG 0.673508 1_3:+10271404 None:intergenic
AATAGTGTTAGTGAAGATGG+TGG 0.675297 1_3:-10273675 Msa0103170:CDS
GAAAGTAAAGATGGTGACGA+GGG 0.680500 1_3:-10271279 Msa0103170:CDS
GATGAAGCGAAGTTTGAACA+GGG 0.683364 1_3:-10271396 Msa0103170:CDS
AAAGTTTAGAGCATAAAACT+GGG 0.706160 1_3:+10271061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTTTTTTTTTTTGA+TGG + chr1_3:10270759-10270778 None:intergenic 10.0%
!!! ATATTGTTAATTTTGTATTT+GGG - chr1_3:10273902-10273921 Msa0103170:intron 10.0%
!! AAAAGAATAGCTTAATTTAA+AGG - chr1_3:10272508-10272527 Msa0103170:intron 15.0%
!! AAAGAATAGCTTAATTTAAA+GGG - chr1_3:10272509-10272528 Msa0103170:intron 15.0%
!!! ATAACATTGAAGTTTTAAAT+TGG + chr1_3:10273026-10273045 None:intergenic 15.0%
!!! CATATTGTTAATTTTGTATT+TGG - chr1_3:10273901-10273920 Msa0103170:intron 15.0%
!! TGGAATTTAATCTGAAAAAA+AGG + chr1_3:10270858-10270877 None:intergenic 20.0%
!! AATGTTAAGTTATTTGTGTT+TGG - chr1_3:10271429-10271448 Msa0103170:CDS 20.0%
!! TGTAATTTAATATGAAGTGT+CGG - chr1_3:10271804-10271823 Msa0103170:intron 20.0%
!! TAGTGATTCATGAATTAATA+GGG - chr1_3:10271849-10271868 Msa0103170:intron 20.0%
!!! GTTTTCTAATCTACATAATT+AGG + chr1_3:10271883-10271902 None:intergenic 20.0%
!!! ACTAATTTTTAGTGTTTCAA+GGG + chr1_3:10272197-10272216 None:intergenic 20.0%
!!! AACTAATTTTTAGTGTTTCA+AGG + chr1_3:10272198-10272217 None:intergenic 20.0%
!! TAGTTGTTAAATGAATACTA+TGG - chr1_3:10272323-10272342 Msa0103170:intron 20.0%
!!! TACTATCATTGTTATTGAAT+TGG - chr1_3:10272767-10272786 Msa0103170:intron 20.0%
!!! TGAAGTTTTAAATTGGATTA+TGG + chr1_3:10273019-10273038 None:intergenic 20.0%
!! AAAACTTCAATGTTATCATT+TGG - chr1_3:10273030-10273049 Msa0103170:intron 20.0%
! CAACAAACAGCAAATAAAAA+TGG + chr1_3:10271198-10271217 None:intergenic 25.0%
!!! ATTTTCATGCTTTAGCTTAT+TGG - chr1_3:10271243-10271262 Msa0103170:CDS 25.0%
!!! TTTTCATGCTTTAGCTTATT+GGG - chr1_3:10271244-10271263 Msa0103170:CDS 25.0%
! AATATGCTCAAAACAACTTA+TGG + chr1_3:10271595-10271614 None:intergenic 25.0%
! TTTCTATAAGAATTCCAAGA+TGG - chr1_3:10271613-10271632 Msa0103170:CDS 25.0%
!!! GTTATTTTGATAAACCATCT+TGG + chr1_3:10271630-10271649 None:intergenic 25.0%
! CTTATACATAAACACTTATG+TGG - chr1_3:10271703-10271722 Msa0103170:intron 25.0%
! ACAACTTTATTAAGCACTTA+CGG + chr1_3:10271741-10271760 None:intergenic 25.0%
! GTAGTGATTCATGAATTAAT+AGG - chr1_3:10271848-10271867 Msa0103170:intron 25.0%
! TAGAAAACAACGATAACTAA+TGG - chr1_3:10271905-10271924 Msa0103170:intron 25.0%
! CATGAAAAATTCTAACATGA+AGG + chr1_3:10272446-10272465 None:intergenic 25.0%
!!! CACTTAAACTGTCAATTTTT+TGG - chr1_3:10272627-10272646 Msa0103170:intron 25.0%
!!! TCTTCAATGGATTCTTTTAA+TGG - chr1_3:10272683-10272702 Msa0103170:intron 25.0%
! TATAAAACCACACAAAAATG+AGG + chr1_3:10272745-10272764 None:intergenic 25.0%
!! AATAATAGTTTCTTGATAGC+TGG - chr1_3:10272818-10272837 Msa0103170:intron 25.0%
! TCTTCCAAACATACAATTAA+AGG + chr1_3:10273087-10273106 None:intergenic 25.0%
!!! TTTTTTCTAATGACTCTGAT+AGG - chr1_3:10273152-10273171 Msa0103170:intron 25.0%
!! AAAGTTTAGAGCATAAAACT+GGG + chr1_3:10273762-10273781 None:intergenic 25.0%
!!! CAAGGAATAAACTCTTATTT+TGG + chr1_3:10273812-10273831 None:intergenic 25.0%
!! TTTTCCTTTTCATTTGTATG+AGG - chr1_3:10273988-10274007 Msa0103170:intron 25.0%
ATGAAAGGTTAAAATGGCAA+CGG + chr1_3:10270788-10270807 None:intergenic 30.0%
ACAGTGATGAAAGGTTAAAA+TGG + chr1_3:10270794-10270813 None:intergenic 30.0%
AAAAGGTTAAGAAGAAAACC+TGG + chr1_3:10270841-10270860 None:intergenic 30.0%
AAACATTTCAGTAAAACGAG+CGG + chr1_3:10270931-10270950 None:intergenic 30.0%
!! TTTTGCTTTTGTGAAGTGAT+AGG - chr1_3:10270950-10270969 Msa0103170:three_prime_UTR 30.0%
!! TTTGCTTTTGTGAAGTGATA+GGG - chr1_3:10270951-10270970 Msa0103170:three_prime_UTR 30.0%
! GTTGCTTCTAATTGTTGATT+AGG - chr1_3:10271214-10271233 Msa0103170:CDS 30.0%
! TTAGCTTATTGGGTTGTATT+TGG - chr1_3:10271254-10271273 Msa0103170:CDS 30.0%
ACACGTTGATACCAATAATA+AGG - chr1_3:10271319-10271338 Msa0103170:CDS 30.0%
CACGTTGATACCAATAATAA+GGG - chr1_3:10271320-10271339 Msa0103170:CDS 30.0%
! ACCAATAATAAGGGTTTGTT+TGG - chr1_3:10271329-10271348 Msa0103170:CDS 30.0%
TCCAAACAAACCCTTATTAT+TGG + chr1_3:10271333-10271352 None:intergenic 30.0%
!!! TTAGTGTATGTTTGGTTTTG+CGG - chr1_3:10271358-10271377 Msa0103170:CDS 30.0%
!! ATCACTATTTAGACTAGCTT+AGG - chr1_3:10271563-10271582 Msa0103170:CDS 30.0%
ACATAAACACTTATGTGGTA+AGG - chr1_3:10271708-10271727 Msa0103170:intron 30.0%
CATAAACACTTATGTGGTAA+GGG - chr1_3:10271709-10271728 Msa0103170:intron 30.0%
!!! TACTTCTTTTTTCCCTACTT+AGG + chr1_3:10271831-10271850 None:intergenic 30.0%
TTCCTTGTTACAAAGACAAA+CGG + chr1_3:10271966-10271985 None:intergenic 30.0%
AAAGGAGATATAGGTTCTTT+CGG + chr1_3:10272040-10272059 None:intergenic 30.0%
! TCAAATTCAGAGTATTTGTC+AGG + chr1_3:10272125-10272144 None:intergenic 30.0%
TTACGATTGACAATATCATG+AGG + chr1_3:10272260-10272279 None:intergenic 30.0%
TCGTAAGATACCTATGATTA+TGG - chr1_3:10272274-10272293 Msa0103170:intron 30.0%
! GTAAAGTTTAGAACTCAATC+AGG + chr1_3:10272716-10272735 None:intergenic 30.0%
AGTACATATCTTCTTGATTC+TGG - chr1_3:10272860-10272879 Msa0103170:intron 30.0%
! TATATTTGTTAGACTGGACA+TGG - chr1_3:10272885-10272904 Msa0103170:intron 30.0%
AAACATACAATTAAAGGCGA+AGG + chr1_3:10273081-10273100 None:intergenic 30.0%
TTCGCCTTTAATTGTATGTT+TGG - chr1_3:10273080-10273099 Msa0103170:intron 30.0%
TAATTGTATGTTTGGAAGAC+TGG - chr1_3:10273088-10273107 Msa0103170:intron 30.0%
! AAGATTTGAGATTAGCTGAA+AGG - chr1_3:10273452-10273471 Msa0103170:intron 30.0%
! AGATTTGAGATTAGCTGAAA+GGG - chr1_3:10273453-10273472 Msa0103170:intron 30.0%
ATTGAGGTAGAAAGTAAAGA+TGG - chr1_3:10273532-10273551 Msa0103170:intron 30.0%
!! AGTCTTGGTTCTTTGATTTT+TGG - chr1_3:10273731-10273750 Msa0103170:CDS 30.0%
! GAAAGTTTAGAGCATAAAAC+TGG + chr1_3:10273763-10273782 None:intergenic 30.0%
TAAGAGTTTATTCCTTGTAG+TGG - chr1_3:10273815-10273834 Msa0103170:CDS 30.0%
AAGAGTTTATTCCTTGTAGT+GGG - chr1_3:10273816-10273835 Msa0103170:CDS 30.0%
ATTGCCTCATACAAATGAAA+AGG + chr1_3:10273995-10274014 None:intergenic 30.0%
! TTTGTATGAGGCAATTGTTT+TGG - chr1_3:10274000-10274019 Msa0103170:intron 30.0%
TTTGAAAAATGCATTCACGA+AGG + chr1_3:10274057-10274076 None:intergenic 30.0%
TTTAACCTTTCATCACTGTC+CGG - chr1_3:10270795-10270814 Msa0103170:three_prime_UTR 35.0%
! CGAGTTTATCAGTGTTTTTC+CGG + chr1_3:10270817-10270836 None:intergenic 35.0%
TTGTGAAGTGATAGGGAAAA+TGG - chr1_3:10270958-10270977 Msa0103170:three_prime_UTR 35.0%
AGGAAAAGAGATGATGAAGA+AGG - chr1_3:10270988-10271007 Msa0103170:three_prime_UTR 35.0%
!! GATGAAGAAGGTTCTGAAAA+AGG - chr1_3:10271000-10271019 Msa0103170:three_prime_UTR 35.0%
!! AAGAAGGTTCTGAAAAAGGT+TGG - chr1_3:10271004-10271023 Msa0103170:three_prime_UTR 35.0%
TCCCAAAAGCAAAGTTGTTA+TGG - chr1_3:10271075-10271094 Msa0103170:three_prime_UTR 35.0%
!! CCCATAACAACTTTGCTTTT+GGG + chr1_3:10271079-10271098 None:intergenic 35.0%
CCCAAAAGCAAAGTTGTTAT+GGG - chr1_3:10271076-10271095 Msa0103170:three_prime_UTR 35.0%
! ACCCATAACAACTTTGCTTT+TGG + chr1_3:10271080-10271099 None:intergenic 35.0%
AATAGTGTTAGTGAAGATGG+TGG - chr1_3:10271145-10271164 Msa0103170:three_prime_UTR 35.0%
! GGATTGACTTAGTGTATGTT+TGG - chr1_3:10271350-10271369 Msa0103170:CDS 35.0%
ATCCGTTTGTCTTTGTAACA+AGG - chr1_3:10271961-10271980 Msa0103170:intron 35.0%
AGTCATGAAATCTGAATGTC+AGG - chr1_3:10272013-10272032 Msa0103170:intron 35.0%
AAGGAGATATAGGTTCTTTC+GGG + chr1_3:10272039-10272058 None:intergenic 35.0%
GCTAATCAGAAAGGAGATAT+AGG + chr1_3:10272049-10272068 None:intergenic 35.0%
TATCCACATGTAAAGTTCTC+TGG - chr1_3:10272077-10272096 Msa0103170:intron 35.0%
AATCCAGAGAACTTTACATG+TGG + chr1_3:10272083-10272102 None:intergenic 35.0%
TAGTGTTTCAAGGGAAACTT+TGG + chr1_3:10272188-10272207 None:intergenic 35.0%
AGGATGTATATAAGCTGACT+AGG + chr1_3:10272240-10272259 None:intergenic 35.0%
ATACCTATGATTATGGTGTG+CGG - chr1_3:10272281-10272300 Msa0103170:intron 35.0%
TGCAAGCAATATAAATTGCG+AGG + chr1_3:10272360-10272379 None:intergenic 35.0%
! TTTCATGATTTTGTCAGCAG+AGG - chr1_3:10272396-10272415 Msa0103170:intron 35.0%
TTAGCTTCTAGTGTTGTATC+TGG - chr1_3:10272468-10272487 Msa0103170:intron 35.0%
!!! AATTTTTTGGAGTATCCGAG+TGG - chr1_3:10272640-10272659 Msa0103170:intron 35.0%
!!! ATTTTTTGGAGTATCCGAGT+GGG - chr1_3:10272641-10272660 Msa0103170:intron 35.0%
! GCTGGCTAAATTTTCTTCAA+TGG - chr1_3:10272670-10272689 Msa0103170:intron 35.0%
!! CTATTGACCTCATTTTTGTG+TGG - chr1_3:10272735-10272754 Msa0103170:intron 35.0%
!! ATTGAATTGGTCTTCTCAAC+TGG - chr1_3:10272780-10272799 Msa0103170:intron 35.0%
!! TTGAATTGGTCTTCTCAACT+GGG - chr1_3:10272781-10272800 Msa0103170:intron 35.0%
!!! GCTGGCTAGTACTAATTTTT+TGG - chr1_3:10272836-10272855 Msa0103170:intron 35.0%
! CTGGCATATATTTGTTAGAC+TGG - chr1_3:10272879-10272898 Msa0103170:intron 35.0%
ATATTCCAAGCAGCATTGTT+GGG + chr1_3:10272913-10272932 None:intergenic 35.0%
AATATTCCAAGCAGCATTGT+TGG + chr1_3:10272914-10272933 None:intergenic 35.0%
! TGGTTGCTCAATTTTGTGTT+AGG - chr1_3:10273050-10273069 Msa0103170:intron 35.0%
AACCTAACGTACAAGATAAG+AGG - chr1_3:10273191-10273210 Msa0103170:intron 35.0%
TAATACGAATGTGACGATCT+AGG + chr1_3:10273231-10273250 None:intergenic 35.0%
GAAGACTCCTTATCAAAAGA+TGG - chr1_3:10273312-10273331 Msa0103170:intron 35.0%
! TCTGTGTCCATCTTTTGATA+AGG + chr1_3:10273322-10273341 None:intergenic 35.0%
CAAAAGATGGACACAGAAAT+AGG - chr1_3:10273325-10273344 Msa0103170:intron 35.0%
GAACAAGCACAAACTAATTG+AGG - chr1_3:10273516-10273535 Msa0103170:intron 35.0%
! AGATTTACTAAGAGTGCATG+AGG + chr1_3:10273851-10273870 None:intergenic 35.0%
GCACTCTTAGTAAATCTAGA+AGG - chr1_3:10273854-10273873 Msa0103170:exon 35.0%
!! CGTGAATGCATTTTTCAAAG+AGG - chr1_3:10274058-10274077 Msa0103170:five_prime_UTR 35.0%
! TTTTTCCGGACAGTGATGAA+AGG + chr1_3:10270803-10270822 None:intergenic 40.0%
AAACACTGATAAACTCGTCC+AGG - chr1_3:10270820-10270839 Msa0103170:three_prime_UTR 40.0%
CAATGGAGTGTAAATTGCGT+TGG + chr1_3:10270878-10270897 None:intergenic 40.0%
TTTCAGTAAAACGAGCGGAA+AGG + chr1_3:10270926-10270945 None:intergenic 40.0%
ATAGGGAAAATGGCGTTTAG+AGG - chr1_3:10270968-10270987 Msa0103170:three_prime_UTR 40.0%
CGAAGGAATCGATAGAGAAA+TGG - chr1_3:10271050-10271069 Msa0103170:three_prime_UTR 40.0%
! CTAAACGTGGTCTTTTTGCT+GGG - chr1_3:10271104-10271123 Msa0103170:three_prime_UTR 40.0%
CTGGGAAACATATCCAGTTT+GGG - chr1_3:10271122-10271141 Msa0103170:three_prime_UTR 40.0%
CACTAACACTATTCCCAAAC+TGG + chr1_3:10271138-10271157 None:intergenic 40.0%
GGGAATAGTGTTAGTGAAGA+TGG - chr1_3:10271142-10271161 Msa0103170:three_prime_UTR 40.0%
! TTAGTGAAGATGGTGGTAAC+AGG - chr1_3:10271152-10271171 Msa0103170:exon 40.0%
ATGTGATGCGCTAATCAGAA+AGG + chr1_3:10272058-10272077 None:intergenic 40.0%
!!! TTTTTTGGAGTATCCGAGTG+GGG - chr1_3:10272642-10272661 Msa0103170:intron 40.0%
TATTCCAAGCAGCATTGTTG+GGG + chr1_3:10272912-10272931 None:intergenic 40.0%
ACAAGAATGAACCTGTTTGC+TGG - chr1_3:10272985-10273004 Msa0103170:intron 40.0%
CTAATGACTCTGATAGGACA+AGG - chr1_3:10273158-10273177 Msa0103170:intron 40.0%
AGCCTCTTATCTTGTACGTT+AGG + chr1_3:10273196-10273215 None:intergenic 40.0%
! ACACAGAAATAGGCGTTTTG+TGG - chr1_3:10273335-10273354 Msa0103170:intron 40.0%
GAGAAGTTGTATGAAGAGCT+TGG - chr1_3:10273370-10273389 Msa0103170:intron 40.0%
TGAAGAGCTTGGAAAGAAAG+AGG - chr1_3:10273381-10273400 Msa0103170:intron 40.0%
! AGAGGTTGTATTCTTTTCCC+CGG - chr1_3:10273399-10273418 Msa0103170:intron 40.0%
GATGAAGCGAAGTTTGAACA+GGG - chr1_3:10273424-10273443 Msa0103170:intron 40.0%
! TAGTTTGTGCTTGTTCATGC+TGG + chr1_3:10273512-10273531 None:intergenic 40.0%
! AGAAAGTAAAGATGGTGACG+AGG - chr1_3:10273540-10273559 Msa0103170:intron 40.0%
! GAAAGTAAAGATGGTGACGA+GGG - chr1_3:10273541-10273560 Msa0103170:intron 40.0%
ACTCGATTCATCAAAGCGAT+TGG - chr1_3:10273591-10273610 Msa0103170:intron 40.0%
GATGTTATGTCAGCTGATGA+CGG - chr1_3:10273674-10273693 Msa0103170:CDS 40.0%
CTCATCGAGTATGAAAGTCT+TGG - chr1_3:10273716-10273735 Msa0103170:CDS 40.0%
GGAAAATTGATCCCACTACA+AGG + chr1_3:10273830-10273849 None:intergenic 40.0%
GAGATATTAGTCACCTGCAT+AGG + chr1_3:10273932-10273951 None:intergenic 40.0%
AGTACCTTATACAAGCCAGA+AGG + chr1_3:10274027-10274046 None:intergenic 40.0%
AGAGATCTGTTACGACGCAA+TGG + chr1_3:10270895-10270914 None:intergenic 45.0%
! GCTAAACGTGGTCTTTTTGC+TGG - chr1_3:10271103-10271122 Msa0103170:three_prime_UTR 45.0%
GCTGGGAAACATATCCAGTT+TGG - chr1_3:10271121-10271140 Msa0103170:three_prime_UTR 45.0%
!! ATGAAGTGTCGGTCCTAAGT+AGG - chr1_3:10271815-10271834 Msa0103170:intron 45.0%
!! TGAAGTGTCGGTCCTAAGTA+GGG - chr1_3:10271816-10271835 Msa0103170:intron 45.0%
!! AAGAAGTGCTCCAAGCTTTG+TGG - chr1_3:10272157-10272176 Msa0103170:intron 45.0%
!! AGAAGTGCTCCAAGCTTTGT+GGG - chr1_3:10272158-10272177 Msa0103170:intron 45.0%
GTTCCGCACACCATAATCAT+AGG + chr1_3:10272287-10272306 None:intergenic 45.0%
! ATTTTGTCAGCAGAGGACAC+CGG - chr1_3:10272403-10272422 Msa0103170:intron 45.0%
TGTGACAGACAAGATCATGC+AGG + chr1_3:10272944-10272963 None:intergenic 45.0%
!! GAACCTGTTTGCTGGTTTAG+TGG - chr1_3:10272993-10273012 Msa0103170:intron 45.0%
CTGATAGGACAAGGAGAACA+TGG - chr1_3:10273167-10273186 Msa0103170:intron 45.0%
TCAAACTTCGCTTCATCCTC+CGG + chr1_3:10273421-10273440 None:intergenic 45.0%
GGATGAAGCGAAGTTTGAAC+AGG - chr1_3:10273423-10273442 Msa0103170:intron 45.0%
GAGGGAGCTGCTAAAATTGA+GGG - chr1_3:10273559-10273578 Msa0103170:intron 45.0%
!!! GGCAATTGTTTTGGACCTTC+TGG - chr1_3:10274009-10274028 Msa0103170:five_prime_UTR 45.0%
!! TGGACCTTCTGGCTTGTATA+AGG - chr1_3:10274020-10274039 Msa0103170:five_prime_UTR 45.0%
GAATATGACTCCACGAGCGA+AGG - chr1_3:10271033-10271052 Msa0103170:three_prime_UTR 50.0%
!! TCTATCGATTCCTTCGCTCG+TGG + chr1_3:10271046-10271065 None:intergenic 50.0%
GTTATGGGTCGTGCTAAACG+TGG - chr1_3:10271091-10271110 Msa0103170:three_prime_UTR 50.0%
TTTGGTCTGCCCACAAAGCT+TGG + chr1_3:10272170-10272189 None:intergenic 50.0%
TGGCCACTAAACCAGCAAAC+AGG + chr1_3:10272999-10273018 None:intergenic 50.0%
TGCCTTGTCGATGCAACGAA+GGG + chr1_3:10273269-10273288 None:intergenic 50.0%
! GGTTGTATTCTTTTCCCCGG+AGG - chr1_3:10273402-10273421 Msa0103170:intron 50.0%
AAACTTCGCTTCATCCTCCG+GGG + chr1_3:10273419-10273438 None:intergenic 50.0%
CAAACTTCGCTTCATCCTCC+GGG + chr1_3:10273420-10273439 None:intergenic 50.0%
CGAGGGAGCTGCTAAAATTG+AGG - chr1_3:10273558-10273577 Msa0103170:intron 50.0%
GGAGCTAACACATACGAGAC+AGG + chr1_3:10273619-10273638 None:intergenic 50.0%
CGTATGTGTTAGCTCCTGAC+AGG - chr1_3:10273623-10273642 Msa0103170:intron 50.0%
TCTCCAACTTTGAGCCTGTC+AGG + chr1_3:10273640-10273659 None:intergenic 50.0%
GTATTTGGGTGCACCTATGC+AGG - chr1_3:10273916-10273935 Msa0103170:intron 50.0%
TATCCGAGTGGGGATGATGC+TGG - chr1_3:10272652-10272671 Msa0103170:intron 55.0%
TAGCCAGCATCATCCCCACT+CGG + chr1_3:10272658-10272677 None:intergenic 55.0%
TGGACCCCAACAATGCTGCT+TGG - chr1_3:10272905-10272924 Msa0103170:intron 55.0%
CTGCCTTGTCGATGCAACGA+AGG + chr1_3:10273270-10273289 None:intergenic 55.0%
! CTTCGTTGCATCGACAAGGC+AGG - chr1_3:10273268-10273287 Msa0103170:intron 55.0%
GCTCCTGACAGGCTCAAAGT+TGG - chr1_3:10273634-10273653 Msa0103170:intron 55.0%
!! GGACACCGGCTCGTCTTCAT+AGG - chr1_3:10272417-10272436 Msa0103170:intron 60.0%
!! CACCGGCTCGTCTTCATAGG+CGG - chr1_3:10272420-10272439 Msa0103170:intron 60.0%
! GATGCAACGAAGGGCGTGAG+TGG + chr1_3:10273260-10273279 None:intergenic 60.0%
! CGCCCTTCGTTGCATCGACA+AGG - chr1_3:10273264-10273283 Msa0103170:intron 60.0%
! CGTTGCATCGACAAGGCAGG+CGG - chr1_3:10273271-10273290 Msa0103170:intron 60.0%
GGCCGCCTATGAAGACGAGC+CGG + chr1_3:10272425-10272444 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 10270750 10274092 10270750 ID=Msa0103170;Name=Msa0103170
chr1_3 mRNA 10270750 10274092 10270750 ID=Msa0103170-mRNA-1;Parent=Msa0103170;Name=Msa0103170-mRNA-1;_AED=0.01;_eAED=0.01;_QI=103|1|1|1|0.5|0.33|3|417|232
chr1_3 exon 10270750 10271668 10270750 ID=Msa0103170-mRNA-1:exon:4247;Parent=Msa0103170-mRNA-1
chr1_3 exon 10273669 10273876 10273669 ID=Msa0103170-mRNA-1:exon:4246;Parent=Msa0103170-mRNA-1
chr1_3 exon 10274001 10274092 10274001 ID=Msa0103170-mRNA-1:exon:4245;Parent=Msa0103170-mRNA-1
chr1_3 five_prime_UTR 10274001 10274092 10274001 ID=Msa0103170-mRNA-1:five_prime_utr;Parent=Msa0103170-mRNA-1
chr1_3 five_prime_UTR 10273866 10273876 10273866 ID=Msa0103170-mRNA-1:five_prime_utr;Parent=Msa0103170-mRNA-1
chr1_3 CDS 10273669 10273865 10273669 ID=Msa0103170-mRNA-1:cds;Parent=Msa0103170-mRNA-1
chr1_3 CDS 10271167 10271668 10271167 ID=Msa0103170-mRNA-1:cds;Parent=Msa0103170-mRNA-1
chr1_3 three_prime_UTR 10270750 10271166 10270750 ID=Msa0103170-mRNA-1:three_prime_utr;Parent=Msa0103170-mRNA-1
Gene Sequence

>Msa0103170

ATGGCGTTTAGAGGAAAAGAGATGATGAAGAAGGTTCTGAAAAAGGTTGGAGACAAGAATATGACTCCACGAGCGAAGGAATCGATAGAGAAATGGCTTCCCAAAAGCAAAGTTGTTATGGGTCGTGCTAAACGTGGTCTTTTTGCTGGGAAACATATCCAGTTTGGGAATAGTGTTAGTGAAGATGGTGGTAACAGGACAAGGAGAACATGGAAACCTAACGTACAAGATAAGAGGCTCTTTAGTTATATCCTAGATCGTCACATTCGTATTAAAGTCACCACTCACGCCCTTCGTTGCATCGACAAGGCAGGCGGAATTGATGAGTACTTGCTGAAGACTCCTTATCAAAAGATGGACACAGAAATAGGCGTTTTGTGGAAGTCAAAGATTGAGAAGTTGTATGAAGAGCTTGGAAAGAAAGAGGTTGTATTCTTTTCCCCGGAGGATGAAGCGAAGTTTGAACAGGGCTTTAAAGATTTGAGATTAGCTGAAAGGGAAGCACGAAAAGAAGTCAGAAAAATAGTGCATGCCAGCATGAACAAGCACAAACTAATTGAGGTAGAAAGTAAAGATGGTGACGAGGGAGCTGCTAAAATTGAGGGAGAAATCTCACTCGATTCATCAAAGCGATTGGTTCCTGTCTCGTATGTGTTAGCTCCTGACAGGCTCAAAGTTGGAGATTATATATCTAATTGA
Protein sequence

>Msa0103170

MAFRGKEMMKKVLKKVGDKNMTPRAKESIEKWLPKSKVVMGRAKRGLFAGKHIQFGNSVSEDGGNRTRRTWKPNVQDKRLFSYILDRHIRIKVTTHALRCIDKAGGIDEYLLKTPYQKMDTEIGVLWKSKIEKLYEELGKKEVVFFSPEDEAKFEQGFKDLRLAEREARKEVRKIVHASMNKHKLIEVESKDGDEGAAKIEGEISLDSSKRLVPVSYVLAPDRLKVGDYISN*