Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0110260 | A0A072TDQ4 | 85.047 | 107 | 12 | 1 | 1 | 107 | 7 | 109 | 2.22e-55 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015750 | Msa0110260 | 0.808687 | 2.740828e-50 | -8.615850e-47 |
| Msa0016570 | Msa0110260 | 0.882738 | 8.094296e-71 | -8.615850e-47 |
| Msa0016580 | Msa0110260 | 0.870718 | 1.180343e-66 | -8.615850e-47 |
| Msa0064970 | Msa0110260 | 0.903891 | 2.168900e-79 | -8.615850e-47 |
| Msa0110260 | Msa0110270 | 0.913419 | 6.289481e-84 | -8.615850e-47 |
| Msa0110260 | Msa0124100 | 0.804175 | 2.449979e-49 | -8.615850e-47 |
| Msa0110260 | Msa0124150 | 0.803349 | 3.636466e-49 | -8.615850e-47 |
| Msa0110260 | Msa0142220 | 0.806014 | 1.010596e-49 | -8.615850e-47 |
| Msa0110260 | Msa0191840 | 0.801171 | 1.021261e-48 | -8.615850e-47 |
| Msa0110260 | Msa0252970 | 0.800472 | 1.418323e-48 | -8.615850e-47 |
| Msa0110260 | Msa0306460 | 0.829345 | 5.421981e-55 | -8.615850e-47 |
| Msa0110260 | Msa0360510 | 0.802841 | 4.633300e-49 | -8.615850e-47 |
| Msa0110260 | Msa0362420 | 0.801816 | 7.531820e-49 | -8.615850e-47 |
| Msa0110260 | Msa0729800 | 0.801584 | 8.404454e-49 | -8.615850e-47 |
| Msa0110260 | Msa0743810 | 0.812200 | 4.777647e-51 | -8.615850e-47 |
| Msa0110260 | Msa0967280 | 0.831412 | 1.693778e-55 | -8.615850e-47 |
| Msa0110260 | Msa0976670 | 0.800659 | 1.299316e-48 | -8.615850e-47 |
| Msa0110260 | Msa1111150 | 0.802462 | 5.547237e-49 | -8.615850e-47 |
| Msa0110260 | Msa1146110 | 0.814350 | 1.611404e-51 | -8.615850e-47 |
| Msa0110260 | Msa1248300 | 0.837203 | 5.968589e-57 | -8.615850e-47 |
| Msa0110260 | Msa1248370 | 0.803727 | 3.036789e-49 | -8.615850e-47 |
| Msa0110260 | Msa1289420 | 0.811124 | 8.189679e-51 | -8.615850e-47 |
| Msa0110260 | Msa1344520 | 0.829894 | 3.986083e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0110260 | MtrunA17_Chr7g0255021 | 35.106 | 94 | 54 | 2 | 14 | 107 | 75 | 161 | 1.28e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTCCAACTTCTTCATTA+AGG | 0.234873 | 1_3:-22906071 | None:intergenic |
| TTCACAAGTGATGTATGATA+TGG | 0.297312 | 1_3:-22906096 | None:intergenic |
| AGGAAACTAACGATACTAAA+AGG | 0.344208 | 1_3:+22906282 | Msa0110260:CDS |
| ATGAGAATTTCATCCTCCTT+TGG | 0.374944 | 1_3:-22906141 | None:intergenic |
| CTCTGATCAATCCCAACAAT+TGG | 0.395664 | 1_3:-22906192 | None:intergenic |
| ATTTCAAAGGATCCAATTGT+TGG | 0.406190 | 1_3:+22906180 | Msa0110260:CDS |
| ATTAACCTTAATGAAGAAGT+TGG | 0.447537 | 1_3:+22906066 | Msa0110260:CDS |
| TTTCAAAGGATCCAATTGTT+GGG | 0.456962 | 1_3:+22906181 | Msa0110260:CDS |
| ATGAAATTCTCATTCAATCA+TGG | 0.497742 | 1_3:+22906151 | Msa0110260:CDS |
| ACACCACAAGTTTGCACTCA+AGG | 0.503579 | 1_3:+22906030 | Msa0110260:CDS |
| AGTTTGCACTCAAGGTGGCT+TGG | 0.506608 | 1_3:+22906038 | Msa0110260:CDS |
| TCACAAGTGATGTATGATAT+GGG | 0.522775 | 1_3:-22906095 | None:intergenic |
| ATCATGGCTCAATATTTCAA+AGG | 0.547059 | 1_3:+22906167 | Msa0110260:CDS |
| GAAAGTAAAATTTCAGCCAA+AGG | 0.574785 | 1_3:+22906125 | Msa0110260:CDS |
| TGGGTTTAAATGGGTAGTAG+TGG | 0.580324 | 1_3:+22905979 | None:intergenic |
| AGTAAAATTTCAGCCAAAGG+AGG | 0.612051 | 1_3:+22906128 | Msa0110260:CDS |
| CCACAAGTTTGCACTCAAGG+TGG | 0.655877 | 1_3:+22906033 | Msa0110260:CDS |
| CCACCTTGAGTGCAAACTTG+TGG | 0.671803 | 1_3:-22906033 | None:intergenic |
| ATTGTTGGGATTGATCAGAG+AGG | 0.736327 | 1_3:+22906195 | Msa0110260:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAACCTTAATGAAGAAGT+TGG | + | chr1_3:22906066-22906085 | Msa0110260:CDS | 25.0% |
| ! | AATTTCCAACTTCTTCATTA+AGG | - | chr1_3:22906074-22906093 | None:intergenic | 25.0% |
| ! | ATGAAATTCTCATTCAATCA+TGG | + | chr1_3:22906151-22906170 | Msa0110260:CDS | 25.0% |
| TCACAAGTGATGTATGATAT+GGG | - | chr1_3:22906098-22906117 | None:intergenic | 30.0% | |
| TTCACAAGTGATGTATGATA+TGG | - | chr1_3:22906099-22906118 | None:intergenic | 30.0% | |
| GAAAGTAAAATTTCAGCCAA+AGG | + | chr1_3:22906125-22906144 | Msa0110260:CDS | 30.0% | |
| ATCATGGCTCAATATTTCAA+AGG | + | chr1_3:22906167-22906186 | Msa0110260:CDS | 30.0% | |
| ATTTCAAAGGATCCAATTGT+TGG | + | chr1_3:22906180-22906199 | Msa0110260:CDS | 30.0% | |
| TTTCAAAGGATCCAATTGTT+GGG | + | chr1_3:22906181-22906200 | Msa0110260:CDS | 30.0% | |
| AGGAAACTAACGATACTAAA+AGG | + | chr1_3:22906282-22906301 | Msa0110260:CDS | 30.0% | |
| AGTAAAATTTCAGCCAAAGG+AGG | + | chr1_3:22906128-22906147 | Msa0110260:CDS | 35.0% | |
| ATGAGAATTTCATCCTCCTT+TGG | - | chr1_3:22906144-22906163 | None:intergenic | 35.0% | |
| ! | ATCAGAGAGGAGATAGTTTT+TGG | + | chr1_3:22906208-22906227 | Msa0110260:CDS | 35.0% |
| CTCTGATCAATCCCAACAAT+TGG | - | chr1_3:22906195-22906214 | None:intergenic | 40.0% | |
| ATTGTTGGGATTGATCAGAG+AGG | + | chr1_3:22906195-22906214 | Msa0110260:CDS | 40.0% | |
| !! | GAGGAGATAGTTTTTGGAAG+AGG | + | chr1_3:22906214-22906233 | Msa0110260:CDS | 40.0% |
| !! | GCGACAAGAATTTTCAAGAG+AGG | + | chr1_3:22906262-22906281 | Msa0110260:CDS | 40.0% |
| ACACCACAAGTTTGCACTCA+AGG | + | chr1_3:22906030-22906049 | Msa0110260:CDS | 45.0% | |
| CCACCTTGAGTGCAAACTTG+TGG | - | chr1_3:22906036-22906055 | None:intergenic | 50.0% | |
| CCACAAGTTTGCACTCAAGG+TGG | + | chr1_3:22906033-22906052 | Msa0110260:CDS | 50.0% | |
| AGTTTGCACTCAAGGTGGCT+TGG | + | chr1_3:22906038-22906057 | Msa0110260:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 22905988 | 22906311 | 22905988 | ID=Msa0110260;Name=Msa0110260 |
| chr1_3 | mRNA | 22905988 | 22906311 | 22905988 | ID=Msa0110260-mRNA-1;Parent=Msa0110260;Name=Msa0110260-mRNA-1;_AED=0.36;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|107 |
| chr1_3 | exon | 22905988 | 22906311 | 22905988 | ID=Msa0110260-mRNA-1:exon:8086;Parent=Msa0110260-mRNA-1 |
| chr1_3 | CDS | 22905988 | 22906311 | 22905988 | ID=Msa0110260-mRNA-1:cds;Parent=Msa0110260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
