AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0515260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515260 MtrunA17_Chr3g0124301 100.000 160 0 0 1 160 1 160 4.99e-120 335
Msa0515260 MtrunA17_Chr3g0115561 39.189 148 82 3 11 152 8 153 1.73e-31 110
Msa0515260 MtrunA17_Chr2g0283801 32.704 159 96 7 1 152 1 155 1.20e-22 88.6
Msa0515260 MtrunA17_Chr2g0316151 29.688 128 85 3 24 151 42 164 3.40e-13 63.9
Msa0515260 MtrunA17_Chr2g0316111 29.688 128 85 3 24 151 42 164 3.82e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515260 AT5G10130.1 46.914 162 81 3 1 159 1 160 6.84e-46 147
Msa0515260 AT4G08685.1 40.000 165 87 4 1 159 1 159 2.70e-36 123
Msa0515260 AT1G78040.1 36.095 169 92 5 1 159 1 163 1.66e-34 119
Msa0515260 AT1G78040.3 36.095 169 92 5 1 159 1 163 1.66e-34 119
Msa0515260 AT1G78040.2 36.095 169 92 5 1 159 1 163 1.66e-34 119
Msa0515260 AT1G29140.1 35.669 157 92 3 4 152 6 161 2.57e-26 98.2
Msa0515260 AT5G45880.1 39.024 123 70 3 25 143 35 156 4.40e-23 90.1
Msa0515260 AT4G18596.1 33.117 154 90 4 1 142 1 153 1.86e-22 88.2
Msa0515260 AT4G18596.2 33.117 154 90 4 1 142 47 199 4.72e-22 88.6

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGATTGTGTTGCTGTTAA+TGG 0.278593 3_4:-66389010 Msa0515260:CDS
AATAAACACAACCTTTGATA+TGG 0.316996 3_4:+66388941 None:intergenic
GTGTATTACACGATGATATT+GGG 0.373900 3_4:+66388491 None:intergenic
GGTGTATTACACGATGATAT+TGG 0.406710 3_4:+66388490 None:intergenic
CACTGGAACATACAACATAA+TGG 0.429786 3_4:-66388563 Msa0515260:CDS
CGTCCATAGTCTTCCTGTCT+TGG 0.431661 3_4:+66388617 None:intergenic
ACAAGTAATATTTGAGGAGT+TGG 0.437901 3_4:+66388344 None:intergenic
TAAAGGGAGGATAGAGATGA+GGG 0.446926 3_4:+66388981 None:intergenic
AAATGCTATGGGTTACTTCA+AGG 0.456909 3_4:-66388392 Msa0515260:CDS
TAGGTGCAAAGGTGGGAATT+CGG 0.460306 3_4:-66388642 Msa0515260:CDS
TGGGAATTCGGTGCCAAGAC+AGG 0.469322 3_4:-66388630 Msa0515260:CDS
TATTGTGATACTTGCCGTGC+TGG 0.471219 3_4:-66388922 Msa0515260:CDS
GTGCCAAGACAGGAAGACTA+TGG 0.475430 3_4:-66388620 Msa0515260:CDS
TCTTCACTATGCAAATGCTA+TGG 0.481660 3_4:-66388404 Msa0515260:CDS
TAGCATTTGCATAGTGAAGA+TGG 0.484441 3_4:+66388407 None:intergenic
GATCTGTTACGCCCAGGGTC+CGG 0.486304 3_4:+66388469 None:intergenic
AACATAGCTGTGGCAAGTAA+AGG 0.486675 3_4:+66388964 None:intergenic
ACATAGCTGTGGCAAGTAAA+GGG 0.489307 3_4:+66388965 None:intergenic
GGAGGATAGAGATGAGGGAA+AGG 0.493157 3_4:+66388986 None:intergenic
AGGAGTTGGTGGCAATATTG+AGG 0.508425 3_4:+66388358 None:intergenic
AATCAGACAAGTAATATTTG+AGG 0.510401 3_4:+66388338 None:intergenic
TGCTGGATCTGTTACGCCCA+GGG 0.512778 3_4:+66388464 None:intergenic
AGTAATATTTGAGGAGTTGG+TGG 0.513246 3_4:+66388347 None:intergenic
GTAGTGAGTGAGCACAATGC+TGG 0.521929 3_4:+66388447 None:intergenic
GAGGATAGAGATGAGGGAAA+GGG 0.521934 3_4:+66388987 None:intergenic
ATGCTGGATCTGTTACGCCC+AGG 0.535067 3_4:+66388463 None:intergenic
CTTCACTATGCAAATGCTAT+GGG 0.535954 3_4:-66388403 Msa0515260:CDS
TCGTGTAATACACCGGACCC+TGG 0.543647 3_4:-66388481 Msa0515260:CDS
TTGCATAGTGAAGATGGTTG+AGG 0.549905 3_4:+66388413 None:intergenic
TTCTTGATTAGGTGCAAAGG+TGG 0.550705 3_4:-66388650 Msa0515260:intron
CGTGTAATACACCGGACCCT+GGG 0.556675 3_4:-66388480 Msa0515260:CDS
TGGTGGCAATATTGAGGCAA+AGG 0.557338 3_4:+66388364 None:intergenic
CATTATGTTGTATGTTCCAG+TGG 0.561706 3_4:+66388564 None:intergenic
GTAAAGGGAGGATAGAGATG+AGG 0.562442 3_4:+66388980 None:intergenic
CATTGCTAATCATCATGGCA+AGG 0.587013 3_4:-66389029 None:intergenic
CGATTCTTGATTAGGTGCAA+AGG 0.587702 3_4:-66388653 Msa0515260:intron
ACAGCTATGTTCCATATCAA+AGG 0.587960 3_4:-66388952 Msa0515260:CDS
GAAGGAGTGACAGATTCCAC+TGG 0.617675 3_4:-66388580 Msa0515260:CDS
TTGGATGTAAAATGTGGCGT+TGG 0.634933 3_4:+66388891 None:intergenic
CGTTGGTTTCGAATCCAGCA+CGG 0.637233 3_4:+66388908 None:intergenic
TCTTGATTAGGTGCAAAGGT+GGG 0.642740 3_4:-66388649 Msa0515260:intron
AATATCATCGTGTAATACAC+CGG 0.667061 3_4:-66388488 Msa0515260:CDS
TTTACCTTGGATGTAAAATG+TGG 0.673598 3_4:+66388885 None:intergenic
TTTGATATGGAACATAGCTG+TGG 0.697489 3_4:+66388954 None:intergenic
TAGCTGTGGCAAGTAAAGGG+AGG 0.702643 3_4:+66388968 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTAAAAAATTTACGTTA+AGG + chr3_4:66388489-66388508 None:intergenic 15.0%
! AATAAACACAACCTTTGATA+TGG + chr3_4:66388404-66388423 None:intergenic 25.0%
! AATCAGACAAGTAATATTTG+AGG + chr3_4:66389007-66389026 None:intergenic 25.0%
TTTACCTTGGATGTAAAATG+TGG + chr3_4:66388460-66388479 None:intergenic 30.0%
TGTAAAATGTACACATGATC+AGG + chr3_4:66388560-66388579 None:intergenic 30.0%
TATTGTGACGATTCTTGATT+AGG - chr3_4:66388681-66388700 Msa0515260:intron 30.0%
! CAGTTTTGCAAATATTGTCA+TGG + chr3_4:66388818-66388837 None:intergenic 30.0%
GTGTATTACACGATGATATT+GGG + chr3_4:66388854-66388873 None:intergenic 30.0%
AATATCATCGTGTAATACAC+CGG - chr3_4:66388854-66388873 Msa0515260:intron 30.0%
ACAAGTAATATTTGAGGAGT+TGG + chr3_4:66389001-66389020 None:intergenic 30.0%
! AAGGATTGTGTTGCTGTTAA+TGG - chr3_4:66388332-66388351 Msa0515260:CDS 35.0%
TTTGATATGGAACATAGCTG+TGG + chr3_4:66388391-66388410 None:intergenic 35.0%
ACAGCTATGTTCCATATCAA+AGG - chr3_4:66388390-66388409 Msa0515260:CDS 35.0%
GTTAAGGTGTGTATTTACCT+TGG + chr3_4:66388473-66388492 None:intergenic 35.0%
! TATGGACGAAGTTTTCTACA+CGG - chr3_4:66388740-66388759 Msa0515260:intron 35.0%
CATTATGTTGTATGTTCCAG+TGG + chr3_4:66388781-66388800 None:intergenic 35.0%
CACTGGAACATACAACATAA+TGG - chr3_4:66388779-66388798 Msa0515260:intron 35.0%
GGTGTATTACACGATGATAT+TGG + chr3_4:66388855-66388874 None:intergenic 35.0%
TAGCATTTGCATAGTGAAGA+TGG + chr3_4:66388938-66388957 None:intergenic 35.0%
TCTTCACTATGCAAATGCTA+TGG - chr3_4:66388938-66388957 Msa0515260:CDS 35.0%
CTTCACTATGCAAATGCTAT+GGG - chr3_4:66388939-66388958 Msa0515260:CDS 35.0%
! AAATGCTATGGGTTACTTCA+AGG - chr3_4:66388950-66388969 Msa0515260:CDS 35.0%
AGTAATATTTGAGGAGTTGG+TGG + chr3_4:66388998-66389017 None:intergenic 35.0%
TAAAGGGAGGATAGAGATGA+GGG + chr3_4:66388364-66388383 None:intergenic 40.0%
ACATAGCTGTGGCAAGTAAA+GGG + chr3_4:66388380-66388399 None:intergenic 40.0%
AACATAGCTGTGGCAAGTAA+AGG + chr3_4:66388381-66388400 None:intergenic 40.0%
TTGGATGTAAAATGTGGCGT+TGG + chr3_4:66388454-66388473 None:intergenic 40.0%
! AACGCCACATTTTACATCCA+AGG - chr3_4:66388453-66388472 Msa0515260:CDS 40.0%
CGATTCTTGATTAGGTGCAA+AGG - chr3_4:66388689-66388708 Msa0515260:intron 40.0%
TTCTTGATTAGGTGCAAAGG+TGG - chr3_4:66388692-66388711 Msa0515260:intron 40.0%
TCTTGATTAGGTGCAAAGGT+GGG - chr3_4:66388693-66388712 Msa0515260:intron 40.0%
GTGCTCACTCACTACAAAAA+TGG - chr3_4:66388903-66388922 Msa0515260:CDS 40.0%
TTGCATAGTGAAGATGGTTG+AGG + chr3_4:66388932-66388951 None:intergenic 40.0%
GAGGATAGAGATGAGGGAAA+GGG + chr3_4:66388358-66388377 None:intergenic 45.0%
GTAAAGGGAGGATAGAGATG+AGG + chr3_4:66388365-66388384 None:intergenic 45.0%
TATTGTGATACTTGCCGTGC+TGG - chr3_4:66388420-66388439 Msa0515260:CDS 45.0%
TAGGTGCAAAGGTGGGAATT+CGG - chr3_4:66388700-66388719 Msa0515260:intron 45.0%
! GACGAAGTTTTCTACACGGA+AGG - chr3_4:66388744-66388763 Msa0515260:intron 45.0%
TGGTGGCAATATTGAGGCAA+AGG + chr3_4:66388981-66389000 None:intergenic 45.0%
!! AGGAGTTGGTGGCAATATTG+AGG + chr3_4:66388987-66389006 None:intergenic 45.0%
GGAGGATAGAGATGAGGGAA+AGG + chr3_4:66388359-66388378 None:intergenic 50.0%
TAGCTGTGGCAAGTAAAGGG+AGG + chr3_4:66388377-66388396 None:intergenic 50.0%
!! CGTTGGTTTCGAATCCAGCA+CGG + chr3_4:66388437-66388456 None:intergenic 50.0%
GTGCCAAGACAGGAAGACTA+TGG - chr3_4:66388722-66388741 Msa0515260:intron 50.0%
CGTCCATAGTCTTCCTGTCT+TGG + chr3_4:66388728-66388747 None:intergenic 50.0%
GAAGGAGTGACAGATTCCAC+TGG - chr3_4:66388762-66388781 Msa0515260:intron 50.0%
GTAGTGAGTGAGCACAATGC+TGG + chr3_4:66388898-66388917 None:intergenic 50.0%
!! TGGGAATTCGGTGCCAAGAC+AGG - chr3_4:66388712-66388731 Msa0515260:intron 55.0%
TCGTGTAATACACCGGACCC+TGG - chr3_4:66388861-66388880 Msa0515260:intron 55.0%
CGTGTAATACACCGGACCCT+GGG - chr3_4:66388862-66388881 Msa0515260:intron 55.0%
TGCTGGATCTGTTACGCCCA+GGG + chr3_4:66388881-66388900 None:intergenic 55.0%
ATGCTGGATCTGTTACGCCC+AGG + chr3_4:66388882-66388901 None:intergenic 55.0%
GATCTGTTACGCCCAGGGTC+CGG + chr3_4:66388876-66388895 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 66388327 66389037 66388327 ID=Msa0515260;Name=Msa0515260
chr3_4 mRNA 66388327 66389037 66388327 ID=Msa0515260-mRNA-1;Parent=Msa0515260;Name=Msa0515260-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|160
chr3_4 exon 66388890 66389037 66388890 ID=Msa0515260-mRNA-1:exon:17082;Parent=Msa0515260-mRNA-1
chr3_4 exon 66388327 66388661 66388327 ID=Msa0515260-mRNA-1:exon:17081;Parent=Msa0515260-mRNA-1
chr3_4 CDS 66388890 66389037 66388890 ID=Msa0515260-mRNA-1:cds;Parent=Msa0515260-mRNA-1
chr3_4 CDS 66388327 66388661 66388327 ID=Msa0515260-mRNA-1:cds;Parent=Msa0515260-mRNA-1
Gene Sequence

>Msa0515260

ATGGCAAGGATTGTGTTGCTGTTAATGGCCCTTTCCCTCATCTCTATCCTCCCTTTACTTGCCACAGCTATGTTCCATATCAAAGGTTGTGTTTATTGTGATACTTGCCGTGCTGGATTCGAAACCAACGCCACATTTTACATCCAAGGTGCAAAGGTGGGAATTCGGTGCCAAGACAGGAAGACTATGGACGAAGTTTTCTACACGGAAGGAGTGACAGATTCCACTGGAACATACAACATAATGGTTGAGAATGATCACCATGACAATATTTGCAAAACTGTGCTTGTTAGCAGCCCAATATCATCGTGTAATACACCGGACCCTGGGCGTAACAGATCCAGCATTGTGCTCACTCACTACAAAAATGGTGTCCTCAACCATCTTCACTATGCAAATGCTATGGGTTACTTCAAGGATGATCCTTTGCCTCAATATTGCCACCAACTCCTCAAATATTACTTGTCTGATTCAGATGTTTGA

Protein sequence

>Msa0515260

MARIVLLLMALSLISILPLLATAMFHIKGCVYCDTCRAGFETNATFYIQGAKVGIRCQDRKTMDEVFYTEGVTDSTGTYNIMVENDHHDNICKTVLVSSPISSCNTPDPGRNRSSIVLTHYKNGVLNHLHYANAMGYFKDDPLPQYCHQLLKYYLSDSDV*