AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0925440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0925440 MtrunA17_Chr6g0488741 97.222 72 2 0 1 72 1 72 5.35e-49 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0925440 AT2G31490.1 81.429 70 13 0 1 70 1 70 3.96e-41 129

Find 40 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAATTAATAATATATT+TGG 0.092183 6_2:-68530908 Msa0925440:three_prime_UTR
AGAATTATCATCATCGAAAA+TGG 0.203994 6_2:-68534281 Msa0925440:exon
CGTCGCAATCTTCTTCTTGT+TGG 0.305084 6_2:-68534172 Msa0925440:CDS
GGTTGTTTGTTTATGTAGTT+TGG 0.314790 6_2:-68530999 Msa0925440:three_prime_UTR
CTTCTTCTTGTTGGTATCTT+CGG 0.319314 6_2:-68534163 Msa0925440:CDS
CAAAACATCTTACTTACTTA+AGG 0.326571 6_2:+68531094 None:intergenic
CTATCAAACCACTCCATGTT+TGG 0.345249 6_2:-68530974 Msa0925440:three_prime_UTR
AAACAAATACATTGAAGATT+GGG 0.354310 6_2:-68534236 Msa0925440:CDS
CATGGTGTAGCCTTGTATTA+AGG 0.381547 6_2:+68531044 None:intergenic
AAAACAAATACATTGAAGAT+TGG 0.399418 6_2:-68534237 Msa0925440:CDS
CATTGCTAATATGAGTAATA+CGG 0.422131 6_2:+68530752 None:intergenic
ATGCCTTTGTAGACGAGAAC+AGG 0.444933 6_2:+68534130 None:intergenic
GTCACTGCAAAGAGCATAAG+AGG 0.460307 6_2:+68530793 None:intergenic
GAGAGAGATCTGAAGGAAGA+AGG 0.485933 6_2:-68534308 Msa0925440:five_prime_UTR
TATATTAGATTGTATTTGCT+AGG 0.487899 6_2:-68530859 Msa0925440:three_prime_UTR
TAAGGCTTGAACTTCCTGTA+TGG 0.491957 6_2:+68531112 None:intergenic
CATAAACAAACAACCCTTGG+TGG 0.495242 6_2:+68531007 None:intergenic
AAGACAATGGCCGACCATAC+AGG 0.509897 6_2:-68531126 Msa0925440:CDS
CTACATAAACAAACAACCCT+TGG 0.515082 6_2:+68531004 None:intergenic
GAATTATCATCATCGAAAAT+GGG 0.518150 6_2:-68534280 Msa0925440:exon
ATCGAAAATGGGTGGAGGAA+TGG 0.519119 6_2:-68534269 Msa0925440:exon
TTGAAGATTGGGGAACTGCT+AGG 0.519133 6_2:-68534225 Msa0925440:CDS
TTTGCTAGGTGAAAAGCTAA+TGG 0.521752 6_2:-68530845 Msa0925440:three_prime_UTR
TGGTTGCAATTTAAAATCAC+AGG 0.525572 6_2:+68530934 None:intergenic
ATCTCGAACACAATTTCCGT+TGG 0.534903 6_2:-68534198 Msa0925440:CDS
TGAAGATTGGGGAACTGCTA+GGG 0.554642 6_2:-68534224 Msa0925440:CDS
GAAAATTAGACCTTAATACA+AGG 0.558400 6_2:-68531054 Msa0925440:three_prime_UTR
GAAGATTGCGACGAGTCCAA+CGG 0.565690 6_2:+68534182 None:intergenic
AACAAATACATTGAAGATTG+GGG 0.581008 6_2:-68534235 Msa0925440:CDS
ATAAACAAACAACCCTTGGT+GGG 0.584072 6_2:+68531008 None:intergenic
GCTTGAACTTCCTGTATGGT+CGG 0.587228 6_2:+68531116 None:intergenic
TCATCATCGAAAATGGGTGG+AGG 0.601325 6_2:-68534274 Msa0925440:exon
AGAGGTAAATACAGTGAGTG+AGG 0.604885 6_2:-68534368 Msa0925440:five_prime_UTR
GTTCCTGTTCTCGTCTACAA+AGG 0.608218 6_2:-68534133 Msa0925440:CDS
TCAACGAAGACAGCCAAACA+TGG 0.619632 6_2:+68530961 None:intergenic
TTATCATCATCGAAAATGGG+TGG 0.631838 6_2:-68534277 Msa0925440:exon
GAAGACAGCCAAACATGGAG+TGG 0.664579 6_2:+68530966 None:intergenic
CCAGGCCCATTGACACAGAG+AGG 0.700364 6_2:-68534386 None:intergenic
ACTGCAAAGAGCATAAGAGG+TGG 0.724683 6_2:+68530796 None:intergenic
CATATGCAAGATGAAGACAA+TGG 0.724975 6_2:-68531139 Msa0925440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTTATTTTCAATTGTTTT+GGG - chr6_2:68531225-68531244 Msa0925440:intron 15.0%
!!! TGTTTTATTATAAGCTATTT+TGG - chr6_2:68532057-68532076 Msa0925440:intron 15.0%
!! TCAATTTATTATAGATAACA+AGG + chr6_2:68533499-68533518 None:intergenic 15.0%
!!! ATATTATTAATTTTGAACCA+TGG + chr6_2:68534228-68534247 None:intergenic 15.0%
!! AAAACAAATACATTGAAGAT+TGG - chr6_2:68530902-68530921 Msa0925440:three_prime_UTR 20.0%
!! AAACAAATACATTGAAGATT+GGG - chr6_2:68530903-68530922 Msa0925440:three_prime_UTR 20.0%
!! ATTTCTGAATAGTTCAATTT+AGG - chr6_2:68531090-68531109 Msa0925440:three_prime_UTR 20.0%
!!! CAACTTATTTTCAATTGTTT+TGG - chr6_2:68531224-68531243 Msa0925440:intron 20.0%
!! AAAAAATAAGTCAATCCAAA+AGG + chr6_2:68531252-68531271 None:intergenic 20.0%
!! GAAAAATTGTGTTTATCTAA+GGG + chr6_2:68531432-68531451 None:intergenic 20.0%
!! TGAAAAATTGTGTTTATCTA+AGG + chr6_2:68531433-68531452 None:intergenic 20.0%
!!! AACACATTTTAGCATTATTT+TGG - chr6_2:68531484-68531503 Msa0925440:intron 20.0%
!!! ACACATTTTAGCATTATTTT+GGG - chr6_2:68531485-68531504 Msa0925440:intron 20.0%
!! TTATTAACAACTCAGAATTT+AGG - chr6_2:68531674-68531693 Msa0925440:intron 20.0%
!! TATTAACAACTCAGAATTTA+GGG - chr6_2:68531675-68531694 Msa0925440:intron 20.0%
!!! CAATTTTTACAAGCTTTTTT+CGG + chr6_2:68532101-68532120 None:intergenic 20.0%
!!! TTCCTAGTTTTGTTAAATAA+TGG - chr6_2:68532961-68532980 Msa0925440:intron 20.0%
!! AATAAGTTGTATATCAAAAC+AGG - chr6_2:68533328-68533347 Msa0925440:intron 20.0%
!! ATAAGTTGTATATCAAAACA+GGG - chr6_2:68533329-68533348 Msa0925440:intron 20.0%
!!! GATACTGATTTATTTTTTAC+TGG - chr6_2:68533539-68533558 Msa0925440:intron 20.0%
!! AATTGTTAAATGATGTGATA+TGG - chr6_2:68533887-68533906 Msa0925440:intron 20.0%
!! TATATTAGATTGTATTTGCT+AGG - chr6_2:68534280-68534299 Msa0925440:exon 20.0%
! AGAATTATCATCATCGAAAA+TGG - chr6_2:68530858-68530877 Msa0925440:three_prime_UTR 25.0%
! GAATTATCATCATCGAAAAT+GGG - chr6_2:68530859-68530878 Msa0925440:three_prime_UTR 25.0%
! AACAAATACATTGAAGATTG+GGG - chr6_2:68530904-68530923 Msa0925440:three_prime_UTR 25.0%
! TAGTTCAATTTAGGAATTTG+AGG - chr6_2:68531099-68531118 Msa0925440:exon 25.0%
! AGTTCAATTTAGGAATTTGA+GGG - chr6_2:68531100-68531119 Msa0925440:exon 25.0%
! ACGTTATTTGAGCTTATATA+TGG - chr6_2:68531130-68531149 Msa0925440:CDS 25.0%
! TGATTATTTCAATCTACCAT+TGG - chr6_2:68531646-68531665 Msa0925440:intron 25.0%
! GTTGTTAATAAATTGACCAA+TGG + chr6_2:68531665-68531684 None:intergenic 25.0%
! CAGAATTTAGGGATTTAATT+AGG - chr6_2:68531686-68531705 Msa0925440:intron 25.0%
! ACAACAGTTCAAAAGAATTT+AGG - chr6_2:68531731-68531750 Msa0925440:intron 25.0%
! CAACAGTTCAAAAGAATTTA+GGG - chr6_2:68531732-68531751 Msa0925440:intron 25.0%
! AACAGTTCAAAAGAATTTAG+GGG - chr6_2:68531733-68531752 Msa0925440:intron 25.0%
!!! AAGAATTTAGGGGTTTTATT+AGG - chr6_2:68531743-68531762 Msa0925440:intron 25.0%
!! AAAGCTTGAAATCCAATTTT+AGG + chr6_2:68532396-68532415 None:intergenic 25.0%
!! AAAATTGGATTTCAAGCTTT+TGG - chr6_2:68532396-68532415 Msa0925440:intron 25.0%
! CAATCTAAACAAATTGAGTA+TGG - chr6_2:68532444-68532463 Msa0925440:intron 25.0%
! TAAATGCTTGCTTGAATTAT+TGG - chr6_2:68532495-68532514 Msa0925440:intron 25.0%
! TGGTTGTAATGTTTAAATTG+TGG - chr6_2:68532515-68532534 Msa0925440:intron 25.0%
! TTAATGCTTGCTTGAATTAT+TGG - chr6_2:68532552-68532571 Msa0925440:intron 25.0%
! AAATCATGTCATCATGTATT+AGG + chr6_2:68532684-68532703 None:intergenic 25.0%
! AGGAACTAAATAGCATATAA+CGG + chr6_2:68532946-68532965 None:intergenic 25.0%
! AGCCATTATTTAACAAAACT+AGG + chr6_2:68532966-68532985 None:intergenic 25.0%
!! CAATTTTAAACCTAACCAAA+AGG + chr6_2:68533116-68533135 None:intergenic 25.0%
!!! CTTTTGGTTAGGTTTAAAAT+TGG - chr6_2:68533114-68533133 Msa0925440:intron 25.0%
! TCAAATAAGCTAATACAAAC+AGG - chr6_2:68533230-68533249 Msa0925440:intron 25.0%
! AATAAAAACAAACGTTAACC+AGG + chr6_2:68533355-68533374 None:intergenic 25.0%
!! AAGTTTAAGATGTTGAATCA+TGG - chr6_2:68533455-68533474 Msa0925440:intron 25.0%
! ATTATAGATAACAAGGTTTG+TGG + chr6_2:68533492-68533511 None:intergenic 25.0%
! ATAAATCAGTATCATGTATG+AGG + chr6_2:68533532-68533551 None:intergenic 25.0%
! CCAAACAATAAAATGAATCA+TGG + chr6_2:68533816-68533835 None:intergenic 25.0%
!! CCATGATTCATTTTATTGTT+TGG - chr6_2:68533813-68533832 Msa0925440:intron 25.0%
!!! ACCTTTTTTTCATGTATGTA+TGG - chr6_2:68533937-68533956 Msa0925440:intron 25.0%
! TCCATACATACATGAAAAAA+AGG + chr6_2:68533941-68533960 None:intergenic 25.0%
! CAAAACATCTTACTTACTTA+AGG + chr6_2:68534048-68534067 None:intergenic 25.0%
!! TAAGATGTTTTGTAATAAGC+CGG - chr6_2:68534056-68534075 Msa0925440:intron 25.0%
! GAAAATTAGACCTTAATACA+AGG - chr6_2:68534085-68534104 Msa0925440:intron 25.0%
AAGAGAGAAGAAGATGAAAA+TGG + chr6_2:68531059-68531078 None:intergenic 30.0%
!! ATAAGTTTTGTGAGCTTGAT+TGG - chr6_2:68531155-68531174 Msa0925440:intron 30.0%
GCAAATAATTAAGCTTGTCA+AGG - chr6_2:68531279-68531298 Msa0925440:intron 30.0%
AAAAATAAGCCAATCCAAAC+TGG - chr6_2:68531406-68531425 Msa0925440:intron 30.0%
!! TTGTTATGAATGCATTGACT+TGG - chr6_2:68531894-68531913 Msa0925440:intron 30.0%
!! ATTTTGATCAAGTCTGTGTT+TGG - chr6_2:68531925-68531944 Msa0925440:intron 30.0%
CTTACATAAGCTTATAGCAT+GGG + chr6_2:68532002-68532021 None:intergenic 30.0%
ATCCAAACAAGTCCTAAAAT+TGG - chr6_2:68532381-68532400 Msa0925440:intron 30.0%
!!! ATCCAATTTTAGGACTTGTT+TGG + chr6_2:68532386-68532405 None:intergenic 30.0%
! GAATTTAAAGTTTGCGATGT+TGG - chr6_2:68532593-68532612 Msa0925440:intron 30.0%
TTTCAAAGCCATAGTATGTA+AGG + chr6_2:68532802-68532821 None:intergenic 30.0%
ACAAACAACAAAACCAATCA+TGG + chr6_2:68533084-68533103 None:intergenic 30.0%
!! ATAAGTTTTGTGAGACTGTT+TGG + chr6_2:68533196-68533215 None:intergenic 30.0%
TACTGGATATTTGAACTACT+AGG - chr6_2:68533556-68533575 Msa0925440:intron 30.0%
TTGAACGTATAATGTGTGTT+TGG + chr6_2:68533662-68533681 None:intergenic 30.0%
TGGAAATTTAAGCTTCATCT+TGG + chr6_2:68533703-68533722 None:intergenic 30.0%
AGGTAGAAGTGAAAACAAAA+TGG + chr6_2:68533921-68533940 None:intergenic 30.0%
! AAGATGTTTTGTAATAAGCC+GGG - chr6_2:68534057-68534076 Msa0925440:intron 30.0%
GGTTGTTTGTTTATGTAGTT+TGG - chr6_2:68534140-68534159 Msa0925440:CDS 30.0%
! TGGTTGCAATTTAAAATCAC+AGG + chr6_2:68534208-68534227 None:intergenic 30.0%
! GTGATTTTAAATTGCAACCA+TGG - chr6_2:68534208-68534227 Msa0925440:CDS 30.0%
TTATCATCATCGAAAATGGG+TGG - chr6_2:68530862-68530881 Msa0925440:three_prime_UTR 35.0%
CTTCTTCTTGTTGGTATCTT+CGG - chr6_2:68530976-68530995 Msa0925440:three_prime_UTR 35.0%
GAAGAAGATGAAAATGGAAG+TGG + chr6_2:68531053-68531072 None:intergenic 35.0%
TCAATTTAGGAATTTGAGGG+AGG - chr6_2:68531103-68531122 Msa0925440:exon 35.0%
!!! TCAATTGTTTTGGGACCTTT+TGG - chr6_2:68531234-68531253 Msa0925440:intron 35.0%
!!! AGCATTATTTTGGGTCTGTT+AGG - chr6_2:68531494-68531513 Msa0925440:intron 35.0%
GCTTACATAAGCTTATAGCA+TGG + chr6_2:68532003-68532022 None:intergenic 35.0%
TCAAATAAGCCAATCCAAAC+AGG - chr6_2:68532183-68532202 Msa0925440:intron 35.0%
!! TATTTTGAGCCTGTTTGGAT+TGG + chr6_2:68532195-68532214 None:intergenic 35.0%
!! AACTGTATTTTGAGCCTGTT+TGG + chr6_2:68532200-68532219 None:intergenic 35.0%
!!! GGATTTCAAGCTTTTGGAAT+CGG - chr6_2:68532402-68532421 Msa0925440:intron 35.0%
CCAATTTACTACAGCTAACA+AGG + chr6_2:68532651-68532670 None:intergenic 35.0%
CCTTGTTAGCTGTAGTAAAT+TGG - chr6_2:68532648-68532667 Msa0925440:intron 35.0%
! GGATTCATGTTTTCTTAGCT+CGG + chr6_2:68532831-68532850 None:intergenic 35.0%
AGAAGGTCTAATTGCTTGTT+TGG + chr6_2:68532852-68532871 None:intergenic 35.0%
ACAAGCAATTAGACCTTCTA+TGG - chr6_2:68532853-68532872 Msa0925440:intron 35.0%
CAAGCAATTAGACCTTCTAT+GGG - chr6_2:68532854-68532873 Msa0925440:intron 35.0%
! AACTTGTTCTAATTTCGCGA+GGG + chr6_2:68532914-68532933 None:intergenic 35.0%
!! TAACTTGTTCTAATTTCGCG+AGG + chr6_2:68532915-68532934 None:intergenic 35.0%
! ATTGACAACCCTAGACTTTT+CGG - chr6_2:68533051-68533070 Msa0925440:intron 35.0%
!! GTTTGTTGCTGAATTCCTTT+TGG - chr6_2:68533098-68533117 Msa0925440:intron 35.0%
!! TTGCTGAATTCCTTTTGGTT+AGG - chr6_2:68533103-68533122 Msa0925440:intron 35.0%
TGAACTACTAGGTGTATGTT+TGG - chr6_2:68533567-68533586 Msa0925440:intron 35.0%
TAGGTGTATGTTTGGTATCA+TGG - chr6_2:68533575-68533594 Msa0925440:intron 35.0%
! TAGCTTTTGCAAAATCACAG+TGG - chr6_2:68533600-68533619 Msa0925440:intron 35.0%
ACGTATAATGTGTGTTTGGT+TGG + chr6_2:68533658-68533677 None:intergenic 35.0%
GTAAGGTCTAATTGCTTGTT+TGG + chr6_2:68533779-68533798 None:intergenic 35.0%
CATATGCAAGATGAAGACAA+TGG - chr6_2:68534000-68534019 Msa0925440:intron 35.0%
! AGATGTTTTGTAATAAGCCG+GGG - chr6_2:68534058-68534077 Msa0925440:intron 35.0%
! AGGTCTAATTTTCATAACCC+CGG + chr6_2:68534078-68534097 None:intergenic 35.0%
! GTGGGTTACGAAAAAATACA+TGG + chr6_2:68534116-68534135 None:intergenic 35.0%
!!! TATTTTTTCGTAACCCACCA+AGG - chr6_2:68534118-68534137 Msa0925440:CDS 35.0%
!!! ATTTTTTCGTAACCCACCAA+GGG - chr6_2:68534119-68534138 Msa0925440:CDS 35.0%
ATAAACAAACAACCCTTGGT+GGG + chr6_2:68534134-68534153 None:intergenic 35.0%
CTACATAAACAAACAACCCT+TGG + chr6_2:68534138-68534157 None:intergenic 35.0%
TTTGCTAGGTGAAAAGCTAA+TGG - chr6_2:68534294-68534313 Msa0925440:five_prime_UTR 35.0%
AGAGGTAAATACAGTGAGTG+AGG - chr6_2:68530771-68530790 Msa0925440:three_prime_UTR 40.0%
ATCTCGAACACAATTTCCGT+TGG - chr6_2:68530941-68530960 Msa0925440:three_prime_UTR 40.0%
TGTTTATCTAAGGGCCAGTT+TGG + chr6_2:68531423-68531442 None:intergenic 40.0%
TTGGGTCTGTTAGGATAAAC+AGG - chr6_2:68531503-68531522 Msa0925440:intron 40.0%
TTTGCACCTTATAGCACAAG+CGG - chr6_2:68531961-68531980 Msa0925440:intron 40.0%
GCTTATTTGAGCTTACCAAC+TGG + chr6_2:68532173-68532192 None:intergenic 40.0%
CTACTAACATAAGCACCTGT+GGG + chr6_2:68532343-68532362 None:intergenic 40.0%
TCTACTAACATAAGCACCTG+TGG + chr6_2:68532344-68532363 None:intergenic 40.0%
! TGGACATTGGAGCTTCATTT+TGG + chr6_2:68532776-68532795 None:intergenic 40.0%
TGAGTTTGACAACCCATAGA+AGG + chr6_2:68532869-68532888 None:intergenic 40.0%
GGGTTGTCAAACTCAAATAG+TGG - chr6_2:68532874-68532893 Msa0925440:intron 40.0%
TTGTATATCAAAACAGGGCC+TGG - chr6_2:68533334-68533353 Msa0925440:intron 40.0%
! TGTGTGTTTGGTTGGTATCA+CGG + chr6_2:68533650-68533669 None:intergenic 40.0%
TTTGTAACATGAGCCAAGCT+AGG - chr6_2:68533742-68533761 Msa0925440:intron 40.0%
! GGATTCTCATTTTCCTAGCT+TGG + chr6_2:68533758-68533777 None:intergenic 40.0%
TAAGGCTTGAACTTCCTGTA+TGG + chr6_2:68534030-68534049 None:intergenic 40.0%
!! CATGGTGTAGCCTTGTATTA+AGG + chr6_2:68534098-68534117 None:intergenic 40.0%
CATAAACAAACAACCCTTGG+TGG + chr6_2:68534135-68534154 None:intergenic 40.0%
CTATCAAACCACTCCATGTT+TGG - chr6_2:68534165-68534184 Msa0925440:CDS 40.0%
AGAGAGAGAGAGAGATCTGA+AGG - chr6_2:68530824-68530843 Msa0925440:three_prime_UTR 45.0%
GAGAGAGATCTGAAGGAAGA+AGG - chr6_2:68530831-68530850 Msa0925440:three_prime_UTR 45.0%
TCATCATCGAAAATGGGTGG+AGG - chr6_2:68530865-68530884 Msa0925440:three_prime_UTR 45.0%
ATCGAAAATGGGTGGAGGAA+TGG - chr6_2:68530870-68530889 Msa0925440:three_prime_UTR 45.0%
TTGAAGATTGGGGAACTGCT+AGG - chr6_2:68530914-68530933 Msa0925440:three_prime_UTR 45.0%
TGAAGATTGGGGAACTGCTA+GGG - chr6_2:68530915-68530934 Msa0925440:three_prime_UTR 45.0%
CGTCGCAATCTTCTTCTTGT+TGG - chr6_2:68530967-68530986 Msa0925440:three_prime_UTR 45.0%
GTTCCTGTTCTCGTCTACAA+AGG - chr6_2:68531006-68531025 Msa0925440:three_prime_UTR 45.0%
ATGCCTTTGTAGACGAGAAC+AGG + chr6_2:68531012-68531031 None:intergenic 45.0%
ATCTAAGGGCCAGTTTGGAT+TGG + chr6_2:68531418-68531437 None:intergenic 45.0%
CTCATGACGCAACATTGTGA+TGG + chr6_2:68531815-68531834 None:intergenic 45.0%
TTGCGATGTTGGAGCATAGA+TGG - chr6_2:68532604-68532623 Msa0925440:intron 45.0%
TGCGATGTTGGAGCATAGAT+GGG - chr6_2:68532605-68532624 Msa0925440:intron 45.0%
AACAAGGTCTGTAGACAGTG+CGG + chr6_2:68532635-68532654 None:intergenic 45.0%
! CTTCATTTTGGACGTGCGTT+TGG + chr6_2:68532764-68532783 None:intergenic 45.0%
GTATGTAAGGAGCTGGACAT+TGG + chr6_2:68532789-68532808 None:intergenic 45.0%
GTCCAGCTCCTTACATACTA+TGG - chr6_2:68532791-68532810 Msa0925440:intron 45.0%
AGCCATAGTATGTAAGGAGC+TGG + chr6_2:68532796-68532815 None:intergenic 45.0%
GTTGTAGCATTGCAACGTAG+TGG - chr6_2:68532994-68533013 Msa0925440:intron 45.0%
TTGCAGACTGCGGAAAATAG+TGG + chr6_2:68533032-68533051 None:intergenic 45.0%
GAGTGAGTCCGAAAAGTCTA+GGG + chr6_2:68533062-68533081 None:intergenic 45.0%
TTTCGGACTCACTCCATGAT+TGG - chr6_2:68533068-68533087 Msa0925440:intron 45.0%
TTCCAGCTTCTCACAAACCT+TGG - chr6_2:68533718-68533737 Msa0925440:intron 45.0%
TGGCTCATGTTACAAAGCCA+AGG + chr6_2:68533738-68533757 None:intergenic 45.0%
TGGAGCGACTCAAGAAAGTA+AGG + chr6_2:68533796-68533815 None:intergenic 45.0%
GCTTGAACTTCCTGTATGGT+CGG + chr6_2:68534026-68534045 None:intergenic 45.0%
TCAACGAAGACAGCCAAACA+TGG + chr6_2:68534181-68534200 None:intergenic 45.0%
! ACTGCAAAGAGCATAAGAGG+TGG + chr6_2:68534346-68534365 None:intergenic 45.0%
! GTCACTGCAAAGAGCATAAG+AGG + chr6_2:68534349-68534368 None:intergenic 45.0%
!! TTCAAAATTAATAATATATT+TGG - chr6_2:68534231-68534250 Msa0925440:CDS 5.0%
GAAGATTGCGACGAGTCCAA+CGG + chr6_2:68530960-68530979 None:intergenic 50.0%
CGACAACCGCTTGTGCTATA+AGG + chr6_2:68531970-68531989 None:intergenic 50.0%
CTCACAAGACTTGTGCCAGT+TGG - chr6_2:68532155-68532174 Msa0925440:intron 50.0%
AGTTCTCCAAACAGTCCCAC+AGG - chr6_2:68532325-68532344 Msa0925440:intron 50.0%
TAAGCACCTGTGGGACTGTT+TGG + chr6_2:68532334-68532353 None:intergenic 50.0%
GGGTTGTCAATTGCAGACTG+CGG + chr6_2:68533042-68533061 None:intergenic 50.0%
GGAGTGAGTCCGAAAAGTCT+AGG + chr6_2:68533063-68533082 None:intergenic 50.0%
AGCCAAGGTTTGTGAGAAGC+TGG + chr6_2:68533723-68533742 None:intergenic 50.0%
AAGACAATGGCCGACCATAC+AGG - chr6_2:68534013-68534032 Msa0925440:intron 50.0%
GAAGACAGCCAAACATGGAG+TGG + chr6_2:68534176-68534195 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 68530764 68534397 68530764 ID=Msa0925440;Name=Msa0925440
chr6_2 mRNA 68530764 68534397 68530764 ID=Msa0925440-mRNA-1;Parent=Msa0925440;Name=Msa0925440-mRNA-1;_AED=0.03;_eAED=0.03;_QI=113|1|1|1|1|1|2|347|72
chr6_2 exon 68530764 68531161 68530764 ID=Msa0925440-mRNA-1:exon:10943;Parent=Msa0925440-mRNA-1
chr6_2 exon 68534117 68534397 68534117 ID=Msa0925440-mRNA-1:exon:10942;Parent=Msa0925440-mRNA-1
chr6_2 five_prime_UTR 68534285 68534397 68534285 ID=Msa0925440-mRNA-1:five_prime_utr;Parent=Msa0925440-mRNA-1
chr6_2 CDS 68534117 68534284 68534117 ID=Msa0925440-mRNA-1:cds;Parent=Msa0925440-mRNA-1
chr6_2 CDS 68531111 68531161 68531111 ID=Msa0925440-mRNA-1:cds;Parent=Msa0925440-mRNA-1
chr6_2 three_prime_UTR 68530764 68531110 68530764 ID=Msa0925440-mRNA-1:three_prime_utr;Parent=Msa0925440-mRNA-1
Gene Sequence

>Msa0925440

ATGGGTGGAGGAATGGAAGCAAAGAAAAACAAATACATTGAAGATTGGGGAACTGCTAGGGAGAATCTCGAACACAATTTCCGTTGGACTCGTCGCAATCTTCTTCTTGTTGGTATCTTCGGTATCGCTGTTCCTGTTCTCGTCTACAAAGGCATCGTCAAAGAATTCCATATGCAAGATGAAGACAATGGCCGACCATACAGGAAGTTCAAGCCTTAA
Protein sequence

>Msa0925440

MGGGMEAKKNKYIEDWGTARENLEHNFRWTRRNLLLVGIFGIAVPVLVYKGIVKEFHMQDEDNGRPYRKFKP*